The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of 46 KDa. Its optimum temperature was 45 to 55 and thermal stability was 55°C for 15 min. The enzyme had optimum pH at 6.5 and stability at a pH range of 5.5 to 7.0 for 24 hr. The maximum activity was observed with substrate concentration of 30 µM and Km was 0.5 mM. The enzyme was strongly inhibited by Cd(+2) and Cu(+2) while it was enhanced by Na(+), Ni(+2), and Mg(+2) at 10 mM while Ca(+2) had slight activation at 20 mM. In addition, the potential application of the L-methioninase as an anticancer agent against various types of tumor cell lines is discussed.
{"title":"Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an Anticancer.","authors":"Mohsen Helmy Selim, El-Zahraa Karm Eldin, Moataza Mahmoud Saad, El-Sayed Eliwa Mostafa, Yosrea Hassan Shetia, Amany Ahmed Hassabo Anise","doi":"10.1155/2015/173140","DOIUrl":"10.1155/2015/173140","url":null,"abstract":"<p><p>The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of 46 KDa. Its optimum temperature was 45 to 55 and thermal stability was 55°C for 15 min. The enzyme had optimum pH at 6.5 and stability at a pH range of 5.5 to 7.0 for 24 hr. The maximum activity was observed with substrate concentration of 30 µM and Km was 0.5 mM. The enzyme was strongly inhibited by Cd(+2) and Cu(+2) while it was enhanced by Na(+), Ni(+2), and Mg(+2) at 10 mM while Ca(+2) had slight activation at 20 mM. In addition, the potential application of the L-methioninase as an anticancer agent against various types of tumor cell lines is discussed. </p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"2 1","pages":"173140"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75975530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"What's hot in hospital design.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"26 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2004-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24400339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As more Americans buy in to Dr. Atkins' commandments to cut out carbohydrates and feast on fats, purveyors of mass packaged food jockey for market primacy with low-carb product intros.
{"title":"Diet Inc.","authors":"William F Gloede","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As more Americans buy in to Dr. Atkins' commandments to cut out carbohydrates and feast on fats, purveyors of mass packaged food jockey for market primacy with low-carb product intros.</p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"25 10","pages":"32-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24405045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
About two-thirds of all adult Americans are fat, but what's alarming is that more children and teenagers are overweight, and as they grow up, their health problems will have huge repercussions for our society.
{"title":"Why we're losing the war against obesity.","authors":"Louise Witt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>About two-thirds of all adult Americans are fat, but what's alarming is that more children and teenagers are overweight, and as they grow up, their health problems will have huge repercussions for our society.</p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"25 10","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24405041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As boomers age, food retailers are faced with weighty questions on what customers want, and whether it's healthy or not.
随着婴儿潮一代年龄的增长，食品零售商面临着消费者想要什么、食品是否健康等重大问题。
{"title":"Trouble in store.","authors":"Peter Francese","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As boomers age, food retailers are faced with weighty questions on what customers want, and whether it's healthy or not.</p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"25 10","pages":"36-7"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24405044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Americans' moral acceptance of doctor-assisted suicide and euthanasia is on the rise, as is their belief that the practices should be legal.
美国人对医生协助自杀和安乐死的道德接受度正在上升，他们认为这些做法应该是合法的。
{"title":"Euthanasia and assisted suicide.","authors":"Pamela Paul","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Americans' moral acceptance of doctor-assisted suicide and euthanasia is on the rise, as is their belief that the practices should be legal.</p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"24 10","pages":"20-1"},"PeriodicalIF":0.0,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22104797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The number of elder Americans with six-figure incomes will rise dramatically over the next five years--a boon to providers of health-care services, the travel industry and makers of luxury products.
{"title":"Older and wealthier.","authors":"Peter Francese","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The number of elder Americans with six-figure incomes will rise dramatically over the next five years--a boon to providers of health-care services, the travel industry and makers of luxury products.</p>","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"24 10","pages":"40-1"},"PeriodicalIF":0.0,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22104799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wanted: one million nurses.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"24 8","pages":"63-5"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22000506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.5040/9780755695560.ch-004
Michael J. Weiss
The 1990s were a decade of less flash and more cash for life's big and little necessities. Tracking the Consumer Expenditure Survey reveals how our shifting tastes and changing demographics are transforming the way we spend.
{"title":"Inconspicuous consumption.","authors":"Michael J. Weiss","doi":"10.5040/9780755695560.ch-004","DOIUrl":"https://doi.org/10.5040/9780755695560.ch-004","url":null,"abstract":"The 1990s were a decade of less flash and more cash for life's big and little necessities. Tracking the Consumer Expenditure Survey reveals how our shifting tastes and changing demographics are transforming the way we spend.","PeriodicalId":79781,"journal":{"name":"American demographics","volume":"24 4 1","pages":"31-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70499311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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