Pub Date : 2022-09-22DOI: 10.53858/arocnpr02021015
I. Adamu, Abafi J. Majiyebo, U. B. Alozieuwa, AbdulRafiu A. Lawal, B. O. Odey, Rahmatallah A. Alawode, E. Berinyuy
Khaya senegalensis (Desr.) A. Juss. is a valuable medicinal plant with various pharmacological and therapeutic properties. Khaya senegalensis has been reportedly used in treating patients with urinary infections, diarrhea, and inflammation. It also has been used for the treatment of liver and kidney diseases. The chemical studies of the plant have revealed that various parts of the plant contain alkaloids, carbohydrates, proteins and amino acids, saponins, glycosides, quinones, flavonoids, terpenoids, etc. Various studies have shown that Khaya senegalensis plays a role in the prevention of cardiovascular disease, lowering blood glucose and serum lipid, decreasing blood pressure and strengthening the heart. This herb has anti-bacterial, anti-malaria, anti-fungal and anti-inflammatory effects. The present review, therefore, revealed that Khaya senegalensis is an important medicinal plant due to its traditional uses for the treatment of several diseases and the presence of many important bioactive compounds which have been implicated in the various pharmacological properties of the plant. Further experimental studies are needed to fully validate the medicinal properties.
{"title":"Ethnopharmacology, chemical constituents, and biological activities of Khaya senegalensis (Desr.) A. Juss a forest plant with multi-purpose applications","authors":"I. Adamu, Abafi J. Majiyebo, U. B. Alozieuwa, AbdulRafiu A. Lawal, B. O. Odey, Rahmatallah A. Alawode, E. Berinyuy","doi":"10.53858/arocnpr02021015","DOIUrl":"https://doi.org/10.53858/arocnpr02021015","url":null,"abstract":"Khaya senegalensis (Desr.) A. Juss. is a valuable medicinal plant with various pharmacological and therapeutic properties. Khaya senegalensis has been reportedly used in treating patients with urinary infections, diarrhea, and inflammation. It also has been used for the treatment of liver and kidney diseases. The chemical studies of the plant have revealed that various parts of the plant contain alkaloids, carbohydrates, proteins and amino acids, saponins, glycosides, quinones, flavonoids, terpenoids, etc. Various studies have shown that Khaya senegalensis plays a role in the prevention of cardiovascular disease, lowering blood glucose and serum lipid, decreasing blood pressure and strengthening the heart. This herb has anti-bacterial, anti-malaria, anti-fungal and anti-inflammatory effects. The present review, therefore, revealed that Khaya senegalensis is an important medicinal plant due to its traditional uses for the treatment of several diseases and the presence of many important bioactive compounds which have been implicated in the various pharmacological properties of the plant. Further experimental studies are needed to fully validate the medicinal properties.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90602337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-22DOI: 10.53858/arocnpr02021626
E. I. Agwupuye, A. Agboola, A. C. Assumpta, L. Ezeanyika, R. Ukpanukpong
Background: The pod of Theobroma cacao is usually processed into a variety of beverages for human consumption; hence this study investigated its safety to vital organs at different doses. Method: Phytochemical, nutrient and antinutrient composition of unfermented theobroma cacao were determined using standard procedures. A total number of twenty-four (24) adult male Wistar albino rats (140 – 200g) were randomly divided into four groups of six rats each and were administered thus: Group I (normal control) rats were intraperitoneally administered with normal saline (1ml/kg b.w./day) for twenty-one days while group II, III and IV were intraperitoneally administered 200mg/kg, 400mg/kg and 600mg/kg b.w/day of Theobroma cacao for twenty-one days. During the course of the treatments, blood was extracted on days 0, 14, and 21 to determine the biochemical parameters including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), while the liver and kidneys of the experimental animals were harvested for histopathological examinations at the end of the treatment. Results: The acute toxicity studies showed no toxicity up to 5000mg/kg of the extract. Treatment with the extract showed no significant difference (p>0.05) in the weight of the rats when compared with the control. A Significant decrease (p≤0.05) was observed in total bilirubin concentration and alanine aminotransferase (ALT) activity of the treated groups when compared with the control group while Aspartate aminotransferase (AST) activity showed a significant increase (p≤0.05) in some of the treated groups. The microscopic examination of the liver and kidney tissues showed no histopathological changes in the extract-treated groups. Conclusion: The observed results showed that unfermented Theobroma cacao is rich in antioxidants and has little or no toxicological effect on albino rats.
{"title":"Evaluation of the toxicological effect of methanol extract of unfermented Theobroma cacao in wistar albino rats","authors":"E. I. Agwupuye, A. Agboola, A. C. Assumpta, L. Ezeanyika, R. Ukpanukpong","doi":"10.53858/arocnpr02021626","DOIUrl":"https://doi.org/10.53858/arocnpr02021626","url":null,"abstract":"Background: The pod of Theobroma cacao is usually processed into a variety of beverages for human consumption; hence this study investigated its safety to vital organs at different doses. Method: Phytochemical, nutrient and antinutrient composition of unfermented theobroma cacao were determined using standard procedures. A total number of twenty-four (24) adult male Wistar albino rats (140 – 200g) were randomly divided into four groups of six rats each and were administered thus: Group I (normal control) rats were intraperitoneally administered with normal saline (1ml/kg b.w./day) for twenty-one days while group II, III and IV were intraperitoneally administered 200mg/kg, 400mg/kg and 600mg/kg b.w/day of Theobroma cacao for twenty-one days. During the course of the treatments, blood was extracted on days 0, 14, and 21 to determine the biochemical parameters including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), while the liver and kidneys of the experimental animals were harvested for histopathological examinations at the end of the treatment. Results: The acute toxicity studies showed no toxicity up to 5000mg/kg of the extract. Treatment with the extract showed no significant difference (p>0.05) in the weight of the rats when compared with the control. A Significant decrease (p≤0.05) was observed in total bilirubin concentration and alanine aminotransferase (ALT) activity of the treated groups when compared with the control group while Aspartate aminotransferase (AST) activity showed a significant increase (p≤0.05) in some of the treated groups. The microscopic examination of the liver and kidney tissues showed no histopathological changes in the extract-treated groups. Conclusion: The observed results showed that unfermented Theobroma cacao is rich in antioxidants and has little or no toxicological effect on albino rats.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79149497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-05DOI: 10.53858/arocnpr02020109
H. Abdulsalami, Y. Daudu, N. U. Adabara, R. Hamzah
Background: Typhoid fever is a serious bacterial infection which causes bacteremia and inflammatory destruction of the intestine and some internal organs in the body. The widespread emergence of multi-drug resistant Salmonella typhi and Salmonella paratyphi has necessitated the search for other therapeutic options. The study was conducted to screen the antisalmonellal activity of Piliostigma thonningii leaf crude extract, fractions and isolated compounds. Methods: The plant leaves were extracted with 70% methanol, the crude extract was partitioned into fractions and was tested for antibacterial activity against S. typhi, S. paratyphi A, S. paratyphi B and Salmonella paratyphi C using agar well diffusion technique. Column and thin layer chromatographic methods were used for phyto-constituent separation of plant extract. The most effective antisalmonellal column chromatography isolated compound was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: The crude extract and the fractions except n-hexane fraction possess antibacterial activity on at least one of the Salmonella strains tested, however, the ethyl acetate fraction (PT1-03) exhibited the widest zone of inhibition on the test bacteria (14-16 mm) at the concentration of 100 mg/ml. The zones of growth inhibition increased with the increasing concentration of the fractions. The corresponding increase in concentration and growth inhibition zone was significant (p<0.05). The isolated compound obtained from the column chromatography also showed significant inhibition on the Salmonella strains (12-15 mm) at the concentration of 50 mg/ml. GC-MS analysis of the column chromatography isolates revealed Levomenthol and hexadecanoic acids as the major compounds. Conclusion: The study clearly indicates that P. thonningii possesses bioactive compounds that are active against some Salmonella species. Therefore, these phytochemicals can be formulated into drugs for the treatment of typhoid and paratyphoid fevers
{"title":"Antisalmonellal Activity and GC-MS Analysis of Piliostigma thonningii leaf extract","authors":"H. Abdulsalami, Y. Daudu, N. U. Adabara, R. Hamzah","doi":"10.53858/arocnpr02020109","DOIUrl":"https://doi.org/10.53858/arocnpr02020109","url":null,"abstract":"Background: Typhoid fever is a serious bacterial infection which causes bacteremia and inflammatory destruction of the intestine and some internal organs in the body. The widespread emergence of multi-drug resistant Salmonella typhi and Salmonella paratyphi has necessitated the search for other therapeutic options. The study was conducted to screen the antisalmonellal activity of Piliostigma thonningii leaf crude extract, fractions and isolated compounds. Methods: The plant leaves were extracted with 70% methanol, the crude extract was partitioned into fractions and was tested for antibacterial activity against S. typhi, S. paratyphi A, S. paratyphi B and Salmonella paratyphi C using agar well diffusion technique. Column and thin layer chromatographic methods were used for phyto-constituent separation of plant extract. The most effective antisalmonellal column chromatography isolated compound was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: The crude extract and the fractions except n-hexane fraction possess antibacterial activity on at least one of the Salmonella strains tested, however, the ethyl acetate fraction (PT1-03) exhibited the widest zone of inhibition on the test bacteria (14-16 mm) at the concentration of 100 mg/ml. The zones of growth inhibition increased with the increasing concentration of the fractions. The corresponding increase in concentration and growth inhibition zone was significant (p<0.05). The isolated compound obtained from the column chromatography also showed significant inhibition on the Salmonella strains (12-15 mm) at the concentration of 50 mg/ml. GC-MS analysis of the column chromatography isolates revealed Levomenthol and hexadecanoic acids as the major compounds. Conclusion: The study clearly indicates that P. thonningii possesses bioactive compounds that are active against some Salmonella species. Therefore, these phytochemicals can be formulated into drugs for the treatment of typhoid and paratyphoid fevers","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76478950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-26DOI: 10.53858/arocnpr02013847
Godwin Gabriel Omula, S. Umar, Samson Adehuga Omogbehin, Tolulope Philip Omotoso
Background: Halea ciliata (Abura leaves) is a medicinal plant and has long been used for the treatment of several diseases including malaria, pains and inflammation, However, no complete pharmacological study to confirm these effects have been reported. In this study, the anti-inflammatory, analgesic, and antimalarial effects of Halea ciliata were investigated. Methods: The leaf of Halea ciliata was extracted with ethanol. The anti-inflammatory activity was tested using the egg albumin induced paw oedema while the analgesic activities were investigated using the hot-plate and Tail flick tests in mice. The anti-malaria activities were evaluated in Plasmodium berghei infected mice. Results: The crude methanol extract of Halea ciliata had LD50 greater than 5000 mg/kg and contains alkaloid, tannin, flavonoid, glycoside, steroid, terpenoid and saponin. In vivo analysis revealed that the crude extract demonstrated 78.64±4.32 % and 78.09±2.34% inhibition of the Plasmodium berghei parasite, while chloroquine, exhibited 92.12±3.45 % activities. The extract also significantly (p<0.05) suppressed the egg albumin induce paw oedema with percentage inhibition of 13.55±0.34, and 11.88±1.34 at 200 and 400 mg/kg respectively, while the standard drug caused 64.40±2.34 inhibition of paw oedema when compared to the non-treated controls. Similarly, the crude extract demonstrated significant analgesic activities in both the hot plate and tail-flick models. Conclusion: These results suggest that crude methanol extract of Halea ciliata has potent anti-malaria, anti-inflammatory and anti-nociceptive activities.
{"title":"Investigations of the anti-inflammatory, analgesic, and anti-malarial activities of extract from the leaf of Halea ciliata (Abura leaves)","authors":"Godwin Gabriel Omula, S. Umar, Samson Adehuga Omogbehin, Tolulope Philip Omotoso","doi":"10.53858/arocnpr02013847","DOIUrl":"https://doi.org/10.53858/arocnpr02013847","url":null,"abstract":"Background: Halea ciliata (Abura leaves) is a medicinal plant and has long been used for the treatment of several diseases including malaria, pains and inflammation, However, no complete pharmacological study to confirm these effects have been reported. In this study, the anti-inflammatory, analgesic, and antimalarial effects of Halea ciliata were investigated. Methods: The leaf of Halea ciliata was extracted with ethanol. The anti-inflammatory activity was tested using the egg albumin induced paw oedema while the analgesic activities were investigated using the hot-plate and Tail flick tests in mice. The anti-malaria activities were evaluated in Plasmodium berghei infected mice. Results: The crude methanol extract of Halea ciliata had LD50 greater than 5000 mg/kg and contains alkaloid, tannin, flavonoid, glycoside, steroid, terpenoid and saponin. In vivo analysis revealed that the crude extract demonstrated 78.64±4.32 % and 78.09±2.34% inhibition of the Plasmodium berghei parasite, while chloroquine, exhibited 92.12±3.45 % activities. The extract also significantly (p<0.05) suppressed the egg albumin induce paw oedema with percentage inhibition of 13.55±0.34, and 11.88±1.34 at 200 and 400 mg/kg respectively, while the standard drug caused 64.40±2.34 inhibition of paw oedema when compared to the non-treated controls. Similarly, the crude extract demonstrated significant analgesic activities in both the hot plate and tail-flick models. Conclusion: These results suggest that crude methanol extract of Halea ciliata has potent anti-malaria, anti-inflammatory and anti-nociceptive activities.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80839470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-26DOI: 10.53858/arocnpr02012837
U. B. Alozieuwa, Abdulahi Mann, A. Kabiru, E. Ogbadoyi
Background: Malaria is a life-threatening disease caused by the protozoan parasite, Plasmodium. The emergence of drug-resistant Plasmodium species to currently available antimalarials has necessitated the search for more effective drugs. This study evaluated the antimalarial potential of the crude extract and fractions of Psidium guajava leaf in Plasmodium berghei infected mice. Method: Mice infected with Plasmodium berghei (P. berghei) were administered orally with the crude extract and fractions at doses ranging from 100-500 and 50-200mg/kg/day respectively, for five consecutive days. Results: The crude extract significantly (p<0.05) inhibited parasite growth as well as prevented body weight loss and packed cell volume reduction dose-dependently. Among the fractions, aqueous fraction was the most active with 54.26% inhibition of parasite growth at 200mg/kg. Remarkable inhibition of parasite growth by the crude extract and aqueous fraction was evident in the prolongation of mice survival relative to the control (27.33±1.76, 24.67±0.67, 28.0±1.16 and 8.33±0.67 for crude extract (500mg/kg), aqueous fraction (200mg/kg), chloroquine and negative control groups respectively). Phytochemical screening of the crude extract revealed the presence of phenol, alkaloids, flavonoids, and terpenoids. Conclusion: The results indicate that crude extract and aqueous fraction of P. guajava leaf are potent antimalarial agent that can be employed in the development of antimalarial drugs.
{"title":"In vivo antimalarial efficacy of Psidium guajava leaf crude extract and fractions in Plasmodium berghei infected mice","authors":"U. B. Alozieuwa, Abdulahi Mann, A. Kabiru, E. Ogbadoyi","doi":"10.53858/arocnpr02012837","DOIUrl":"https://doi.org/10.53858/arocnpr02012837","url":null,"abstract":"Background: Malaria is a life-threatening disease caused by the protozoan parasite, Plasmodium. The emergence of drug-resistant Plasmodium species to currently available antimalarials has necessitated the search for more effective drugs. This study evaluated the antimalarial potential of the crude extract and fractions of Psidium guajava leaf in Plasmodium berghei infected mice. Method: Mice infected with Plasmodium berghei (P. berghei) were administered orally with the crude extract and fractions at doses ranging from 100-500 and 50-200mg/kg/day respectively, for five consecutive days. Results: The crude extract significantly (p<0.05) inhibited parasite growth as well as prevented body weight loss and packed cell volume reduction dose-dependently. Among the fractions, aqueous fraction was the most active with 54.26% inhibition of parasite growth at 200mg/kg. Remarkable inhibition of parasite growth by the crude extract and aqueous fraction was evident in the prolongation of mice survival relative to the control (27.33±1.76, 24.67±0.67, 28.0±1.16 and 8.33±0.67 for crude extract (500mg/kg), aqueous fraction (200mg/kg), chloroquine and negative control groups respectively). Phytochemical screening of the crude extract revealed the presence of phenol, alkaloids, flavonoids, and terpenoids. Conclusion: The results indicate that crude extract and aqueous fraction of P. guajava leaf are potent antimalarial agent that can be employed in the development of antimalarial drugs.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84636726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.53858/arocnpr02012127
Junaidu Isah Goronyo, Y. Ibrahim, B. Tytler, Mujahid Hussaini
Background: Trypanosomiasis is a disease of vertebral animals caused by parasitic protozoa of the genus Trypanosoma. It is one of the neglected tropical diseases (NTDs) affecting about 36 countries of Sub-saharan Africa, threatening more than 60 million people and 70 million animals. Chemotherapy is the major means of controlling African trypanosomiasis is limited by rapid drug resistance, toxicity and high cost. There is an urgent need for therapeutic agents that are effective, affordable, and accessible to the rural poor people in Africa who are greatly affected by the disease. This study aimed to determine the in vivo effect of stem bark methanol extract of Acacia nilotica (A. nilotica) on experimental Trypanosoma brucei brucei infection in Wistar rats. Methods: Phytochemical analysis, and LD50 determination were carried out using standard procedures. Three (3) days pre-patent period was observed after inoculating the rats with the parasite. Parasitemia was monitored daily while the Parked Cell Volume (PCV) was determined at one-day intervals during the infection course. Results: The phytochemical analysis showed the presence of carbohydrates, steroid/triterpenes, saponin, alkaloid, flavonoid, tannin, glycosides, and anthraquinones. The toxicity of the stem bark methanol extract was tolerable at 1200 mg/kg body weight. Methanol extract of A. nilotica (stem bark) showed promising curative potential in vivo against Trypanosoma brucei brucei at 300, 400 and 500 mg/kg body weight. These doses completely cured the experimental T.b.b infection in Wistar rats after 3 days of treatment. Significant reduction (p<0.05) in the parasite burden confirmed by the absence of anaemia (PCV 48.1% ±1.5% and 46.4% ±1.3% respectively) was observed when compared with the “infected but not treated” control group (normal saline group). Conclusion: Based on these observations, it was therefore deduced that the methanol extract of Acacia nilotica stem bark extract possessed the active ingredient that cures experimental T. brucei brucei infections in Wistar rats.
{"title":"In vivo antitrypanosomal activities of Acacia nilotica stem bark methanol extract in Wistar rats infected with Trypanosoma brucei brucei","authors":"Junaidu Isah Goronyo, Y. Ibrahim, B. Tytler, Mujahid Hussaini","doi":"10.53858/arocnpr02012127","DOIUrl":"https://doi.org/10.53858/arocnpr02012127","url":null,"abstract":"Background: Trypanosomiasis is a disease of vertebral animals caused by parasitic protozoa of the genus Trypanosoma. It is one of the neglected tropical diseases (NTDs) affecting about 36 countries of Sub-saharan Africa, threatening more than 60 million people and 70 million animals. Chemotherapy is the major means of controlling African trypanosomiasis is limited by rapid drug resistance, toxicity and high cost. There is an urgent need for therapeutic agents that are effective, affordable, and accessible to the rural poor people in Africa who are greatly affected by the disease. This study aimed to determine the in vivo effect of stem bark methanol extract of Acacia nilotica (A. nilotica) on experimental Trypanosoma brucei brucei infection in Wistar rats. Methods: Phytochemical analysis, and LD50 determination were carried out using standard procedures. Three (3) days pre-patent period was observed after inoculating the rats with the parasite. Parasitemia was monitored daily while the Parked Cell Volume (PCV) was determined at one-day intervals during the infection course. Results: The phytochemical analysis showed the presence of carbohydrates, steroid/triterpenes, saponin, alkaloid, flavonoid, tannin, glycosides, and anthraquinones. The toxicity of the stem bark methanol extract was tolerable at 1200 mg/kg body weight. Methanol extract of A. nilotica (stem bark) showed promising curative potential in vivo against Trypanosoma brucei brucei at 300, 400 and 500 mg/kg body weight. These doses completely cured the experimental T.b.b infection in Wistar rats after 3 days of treatment. Significant reduction (p<0.05) in the parasite burden confirmed by the absence of anaemia (PCV 48.1% ±1.5% and 46.4% ±1.3% respectively) was observed when compared with the “infected but not treated” control group (normal saline group). Conclusion: Based on these observations, it was therefore deduced that the methanol extract of Acacia nilotica stem bark extract possessed the active ingredient that cures experimental T. brucei brucei infections in Wistar rats.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86549926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-28DOI: 10.53858/arocnpr02011320
M. B. Umar, H. Abdulsalami, Rasaq Bolakale Isiaq, Nwamaka Maureen Odu, F. Muhammad
Background: Malaria is undoubtedly one of the world’s deadliest infectious diseases. It is a widespread disease that continues to be associated with considerable morbidity and mortality with a significant social and economic impact in developing countries. This study was aimed at evaluating the therapeutic effect of the ethyl acetate extract of Cassia occidentalis in Plasmodium berghei infected mice. Methods: Qualitative phytochemical screening was carried out, using standard methods. Acute oral toxicity (LD50), as well as both suppressive and curative antiplasmodial test, was carried out. Results: Flavonoids, saponins, steroids, tannins and phenolic compounds were found to be present in the extract. The median lethal dose (LD50) was calculated to be ≥ 5000mg kg-1 body weight. Percentage suppression of parasitaemia was calculated to be 37.84, 42.84, 77.88, 83.06 and 0.00 %, with mean survival period (in days) of 6.00±0.58, 12.33±1.45, 26.33±2.03, 29.00±1.00 and 4.33±0.88 for groups treated with 100, 200, 400 mg kg-1 bodyweight of the extract, 5 mg kg-1 body weight of chloroquine and negative control group respectively. The percentage inhibition of parasitaemia in the curative test was calculated to be 42.38, 47.67, 79.17, 81.99 and 0.00 %, with mean survival period (in days) calculated to be 7.67±1.45, 15.00± 1.73, 25.33±1.86, 29.00±1.00 and 5.00± 1.53 for groups treated with 100, 200, 400 mg kg-1 bodyweight of the extract, 5 mg kg-1 body weight of chloroquine and the negative control group respectively. It was observed that there was a significant (p<0.05) increase in percentage packed cell volume (PCV) in the treated groups compared to the untreated group. Conclusion: The result of this study showed that ethyl acetate extract of Cassia occidentalis leaf possess appreciable antiplasmodial effect and justifies its traditional use as an herbal remedy against malaria and may, therefore; offer a potential drug lead for the development of a safe, effective and affordable antimalarial.
{"title":"Evaluation of the in vivo anti-plasmodial activity of ethyl-acetate extract of Cassia occidentalis leaf in Plasmodium berghei – infected mice","authors":"M. B. Umar, H. Abdulsalami, Rasaq Bolakale Isiaq, Nwamaka Maureen Odu, F. Muhammad","doi":"10.53858/arocnpr02011320","DOIUrl":"https://doi.org/10.53858/arocnpr02011320","url":null,"abstract":"Background: Malaria is undoubtedly one of the world’s deadliest infectious diseases. It is a widespread disease that continues to be associated with considerable morbidity and mortality with a significant social and economic impact in developing countries. This study was aimed at evaluating the therapeutic effect of the ethyl acetate extract of Cassia occidentalis in Plasmodium berghei infected mice. Methods: Qualitative phytochemical screening was carried out, using standard methods. Acute oral toxicity (LD50), as well as both suppressive and curative antiplasmodial test, was carried out. Results: Flavonoids, saponins, steroids, tannins and phenolic compounds were found to be present in the extract. The median lethal dose (LD50) was calculated to be ≥ 5000mg kg-1 body weight. Percentage suppression of parasitaemia was calculated to be 37.84, 42.84, 77.88, 83.06 and 0.00 %, with mean survival period (in days) of 6.00±0.58, 12.33±1.45, 26.33±2.03, 29.00±1.00 and 4.33±0.88 for groups treated with 100, 200, 400 mg kg-1 bodyweight of the extract, 5 mg kg-1 body weight of chloroquine and negative control group respectively. The percentage inhibition of parasitaemia in the curative test was calculated to be 42.38, 47.67, 79.17, 81.99 and 0.00 %, with mean survival period (in days) calculated to be 7.67±1.45, 15.00± 1.73, 25.33±1.86, 29.00±1.00 and 5.00± 1.53 for groups treated with 100, 200, 400 mg kg-1 bodyweight of the extract, 5 mg kg-1 body weight of chloroquine and the negative control group respectively. It was observed that there was a significant (p<0.05) increase in percentage packed cell volume (PCV) in the treated groups compared to the untreated group. Conclusion: The result of this study showed that ethyl acetate extract of Cassia occidentalis leaf possess appreciable antiplasmodial effect and justifies its traditional use as an herbal remedy against malaria and may, therefore; offer a potential drug lead for the development of a safe, effective and affordable antimalarial.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74571841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-26DOI: 10.53858/arocnpr02010112
B. T. Ogunyemi, O. Oderinlo
Background: The ongoing coronavirus pandemic poses a significant social, economic, and health threat worldwide. The situation is exacerbated further by vaccine hesitancy and the ongoing development of mutant strains that could lead to drug resistance. It is therefore critical to find new anti-viral chemotherapeutic agents to reduce or end the epidemic. This study aimed to investigate the antiprotease activity of the oxoaporphine alkaloids in Xylopia aethiopica. Methods: Computational techniques such as molecular docking were used to probe the oxoaporphine alkaloids in the plant for their ability to inhibit the main protease of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The docking score calculations which quantifies the predictive binding affinity of both ligand and target was carried out using Auto-Dock Vina software. Results: The results showed that oxoaporphine alkaloids had a better binding affinity than hydroxychloroquine sulfate (standard). Similarly, the values of the chemical descriptors obtained for these alkaloids revealed notable profiles, and these alkaloids also have good oral bioavailability according to rule of five. Conclusion: These findings imply that these plant-based alkaloids could be investigated further as prospective leads against SARS-CoV-2 main protease. Furthermore, structural-activity relationships on these compounds could be an effective way to mitigate the predicted side effects
{"title":"In-silico investigation of oxoaporphine alkaloids of Xylopia aethiopica against SARS-COV-2 main protease","authors":"B. T. Ogunyemi, O. Oderinlo","doi":"10.53858/arocnpr02010112","DOIUrl":"https://doi.org/10.53858/arocnpr02010112","url":null,"abstract":"Background: The ongoing coronavirus pandemic poses a significant social, economic, and health threat worldwide. The situation is exacerbated further by vaccine hesitancy and the ongoing development of mutant strains that could lead to drug resistance. It is therefore critical to find new anti-viral chemotherapeutic agents to reduce or end the epidemic. This study aimed to investigate the antiprotease activity of the oxoaporphine alkaloids in Xylopia aethiopica. Methods: Computational techniques such as molecular docking were used to probe the oxoaporphine alkaloids in the plant for their ability to inhibit the main protease of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The docking score calculations which quantifies the predictive binding affinity of both ligand and target was carried out using Auto-Dock Vina software. Results: The results showed that oxoaporphine alkaloids had a better binding affinity than hydroxychloroquine sulfate (standard). Similarly, the values of the chemical descriptors obtained for these alkaloids revealed notable profiles, and these alkaloids also have good oral bioavailability according to rule of five. Conclusion: These findings imply that these plant-based alkaloids could be investigated further as prospective leads against SARS-CoV-2 main protease. Furthermore, structural-activity relationships on these compounds could be an effective way to mitigate the predicted side effects","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90674842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-08DOI: 10.53858/arocnpr01023646
Toheeb D. Yissa, W. Okunowo, Rukayat I. Afolayan, Abdulakeem R. Agboola, H. Y. Lukman, Al-Rumman Suleiman, Abafi J. Majiyebo
Background: The purpose of this study was to determine the phytochemical composition and antimicrobial potential of crude n-hexane, ethyl-acetate, methanol and aqueous extracts of Calotropis procera leaves against food spoilage microorganisms. Methods: Standard protocols were employed for the analysis of qualitative phytochemical compositions of the extracts, and antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Pseudomonas aerugenosa and Aspergillus niger. Results: Phytochemical analysis revealed the presence of tannin, saponin, alkaloids, flavonoids, reducing sugar and phenolics. Terpenoids were absent in ethyl acetate and n-hexane extracts while cardiac glycoside was absent in all extracts. All extracts produced antimicrobial activity at a varying zone of inhibition. The widest inhibition zone was produced by methanol extract (21.35±0.43 mm) on staphylococcus aureus while the lowest inhibition zone (12.05±0.45 mm) was observed in the n-hexane fraction. Similarly, the widest inhibition zone (17.24±0.95 mm) was produced by methanol on A. niger while the lowest inhibition zone (5.45±0.42 mm) was produced on n-hexane on A. niger. However, the ethyl acetate extract showed no visible inhibitory zone on all the tested microorganisms. The minimum inhibitory concentration ranged from 32 mg/ml (S. aureus and B. cereus) for ethanol extracts to 128 mg/ml (B. cereus, P. aerugenosa and A. niger) for n-hexane extract. Conclusion: The result shows that the plant is a good source of bioactive compounds that can be used as a natural alternative to a chemical agent in preserving and controlling food poisoning organisms.
{"title":"Phytochemical compositions and antimicrobial activity of leaf extracts of Calotropis procera against food spoilage microorganisms","authors":"Toheeb D. Yissa, W. Okunowo, Rukayat I. Afolayan, Abdulakeem R. Agboola, H. Y. Lukman, Al-Rumman Suleiman, Abafi J. Majiyebo","doi":"10.53858/arocnpr01023646","DOIUrl":"https://doi.org/10.53858/arocnpr01023646","url":null,"abstract":"Background: The purpose of this study was to determine the phytochemical composition and antimicrobial potential of crude n-hexane, ethyl-acetate, methanol and aqueous extracts of Calotropis procera leaves against food spoilage microorganisms. Methods: Standard protocols were employed for the analysis of qualitative phytochemical compositions of the extracts, and antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Pseudomonas aerugenosa and Aspergillus niger. Results: Phytochemical analysis revealed the presence of tannin, saponin, alkaloids, flavonoids, reducing sugar and phenolics. Terpenoids were absent in ethyl acetate and n-hexane extracts while cardiac glycoside was absent in all extracts. All extracts produced antimicrobial activity at a varying zone of inhibition. The widest inhibition zone was produced by methanol extract (21.35±0.43 mm) on staphylococcus aureus while the lowest inhibition zone (12.05±0.45 mm) was observed in the n-hexane fraction. Similarly, the widest inhibition zone (17.24±0.95 mm) was produced by methanol on A. niger while the lowest inhibition zone (5.45±0.42 mm) was produced on n-hexane on A. niger. However, the ethyl acetate extract showed no visible inhibitory zone on all the tested microorganisms. The minimum inhibitory concentration ranged from 32 mg/ml (S. aureus and B. cereus) for ethanol extracts to 128 mg/ml (B. cereus, P. aerugenosa and A. niger) for n-hexane extract. Conclusion: The result shows that the plant is a good source of bioactive compounds that can be used as a natural alternative to a chemical agent in preserving and controlling food poisoning organisms.","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87896206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-14DOI: 10.53858/arocnpr01022935
C. Onuoha, Charity Chinenye Nwachukwu, Ruth Tochukwu Nwachukwu, Chinwe Glory Nwogu Nwogu, C. Chukwudoruo, F. N. Ujowundu
Background: Annona muricata Linn is a notable, well-studied plant of therapeutic value. Based on the abundant pharmacological constituents contained in the understudied plant, it is imperative that the plant parts are investigated for nutritional value and anti-sickling potentials. Sickle cell anaemia (SCA) is an inheritable haematological disorder, caused by an amino acid substitution on the haem protein. The outcomes for SCA are poor health indices and high mortality. Therefore, the use of natural products is necessary and widely promoted in countries with poor health infrastructure. Methods: In this study, A. muricata seeds and leaves were comparatively analysed for the proximate compositions. In addition, aqueous and ethanol extracts of A. muricata seeds and leaves were respectively analysed for anti-sickling potentials with the use of spectrophotometry. Results: Proximate composition of A. muricata seeds and leaves showed that both plant parts contain ash, carbohydrate, fat, fibre, moisture and protein. However, percentage proximate composition of A. muricata seeds was not significantly different from the percentage proximate composition of A. muricata leaves (p ≤ 0.05). From anti-sickling analysis, the aqueous extracts of A. muricata seeds and leaves were observed to inhibit HbSS polymerisation, while the ethanol extracts of A. muricata seeds and leaves showed limitations to the inhibition of HbSS polymerisation. Conclusion: A. muricata seeds and leaves possess potentials as health or nutritional supplements for the management of SCA. Further studies are necessary in order to ascertain efficacy and safety in in vivo models
{"title":"Comparative evaluation of proximate composition and anti-sickling potential of Annona muricata Linn seeds and leaves","authors":"C. Onuoha, Charity Chinenye Nwachukwu, Ruth Tochukwu Nwachukwu, Chinwe Glory Nwogu Nwogu, C. Chukwudoruo, F. N. Ujowundu","doi":"10.53858/arocnpr01022935","DOIUrl":"https://doi.org/10.53858/arocnpr01022935","url":null,"abstract":"Background: Annona muricata Linn is a notable, well-studied plant of therapeutic value. Based on the abundant pharmacological constituents contained in the understudied plant, it is imperative that the plant parts are investigated for nutritional value and anti-sickling potentials. Sickle cell anaemia (SCA) is an inheritable haematological disorder, caused by an amino acid substitution on the haem protein. The outcomes for SCA are poor health indices and high mortality. Therefore, the use of natural products is necessary and widely promoted in countries with poor health infrastructure. Methods: In this study, A. muricata seeds and leaves were comparatively analysed for the proximate compositions. In addition, aqueous and ethanol extracts of A. muricata seeds and leaves were respectively analysed for anti-sickling potentials with the use of spectrophotometry. Results: Proximate composition of A. muricata seeds and leaves showed that both plant parts contain ash, carbohydrate, fat, fibre, moisture and protein. However, percentage proximate composition of A. muricata seeds was not significantly different from the percentage proximate composition of A. muricata leaves (p ≤ 0.05). From anti-sickling analysis, the aqueous extracts of A. muricata seeds and leaves were observed to inhibit HbSS polymerisation, while the ethanol extracts of A. muricata seeds and leaves showed limitations to the inhibition of HbSS polymerisation. Conclusion: A. muricata seeds and leaves possess potentials as health or nutritional supplements for the management of SCA. Further studies are necessary in order to ascertain efficacy and safety in in vivo models","PeriodicalId":8396,"journal":{"name":"AROC in Natural Products Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74178402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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