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Genome Reduction Uncovers a Large Dispensable Genome and Adaptive Role for Copy Number Variation in Asexually Propagated Solanum tuberosum. 基因组还原发现无性繁殖的茄属植物中存在大量可废弃的基因组以及拷贝数变异的适应作用。
IF 11.6 Pub Date : 2016-02-01 Epub Date: 2016-01-15 DOI: 10.1105/tpc.15.00538
Michael A Hardigan, Emily Crisovan, John P Hamilton, Jeongwoon Kim, Parker Laimbeer, Courtney P Leisner, Norma C Manrique-Carpintero, Linsey Newton, Gina M Pham, Brieanne Vaillancourt, Xueming Yang, Zixian Zeng, David S Douches, Jiming Jiang, Richard E Veilleux, C Robin Buell

Clonally reproducing plants have the potential to bear a significantly greater mutational load than sexually reproducing species. To investigate this possibility, we examined the breadth of genome-wide structural variation in a panel of monoploid/doubled monoploid clones generated from native populations of diploid potato (Solanum tuberosum), a highly heterozygous asexually propagated plant. As rare instances of purely homozygous clones, they provided an ideal set for determining the degree of structural variation tolerated by this species and deriving its minimal gene complement. Extensive copy number variation (CNV) was uncovered, impacting 219.8 Mb (30.2%) of the potato genome with nearly 30% of genes subject to at least partial duplication or deletion, revealing the highly heterogeneous nature of the potato genome. Dispensable genes (>7000) were associated with limited transcription and/or a recent evolutionary history, with lower deletion frequency observed in genes conserved across angiosperms. Association of CNV with plant adaptation was highlighted by enrichment in gene clusters encoding functions for environmental stress response, with gene duplication playing a part in species-specific expansions of stress-related gene families. This study revealed unique impacts of CNV in a species with asexual reproductive habits and how CNV may drive adaption through evolution of key stress pathways.

与有性繁殖的物种相比,克隆繁殖的植物有可能承受更大的变异负荷。为了研究这种可能性,我们考察了从二倍体马铃薯(Solanum tuberosum)(一种高度杂合的无性繁殖植物)的本地种群中产生的单倍体/双单倍体克隆的全基因组结构变异的广度。作为罕见的纯同源克隆,它们为确定该物种可容忍的结构变异程度和推导其最小基因补体提供了理想的集合。研究发现了广泛的拷贝数变异(CNV),影响了219.8 Mb(30.2%)的马铃薯基因组,近30%的基因至少有部分重复或缺失,揭示了马铃薯基因组的高度异质性。不需要的基因(大于 7000 个)与转录受限和/或最近的进化历史有关,在被子植物中保留下来的基因的删除频率较低。CNV 与植物适应性的关系突出表现在编码环境胁迫响应功能的基因簇的富集上,基因重复在胁迫相关基因家族的物种特异性扩增中发挥了作用。这项研究揭示了 CNV 对无性繁殖习性物种的独特影响,以及 CNV 如何通过关键应激途径的进化推动适应性。
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引用次数: 0
Improving prevention of depression and anxiety disorders: Repetitive negative thinking as a promising target 改善抑郁症和焦虑症的预防:重复消极思维是一个有希望的目标
Maurice Topper, Paul M.G. Emmelkamp, Thomas Ehring

Prevention of depression and anxiety disorders is widely acknowledged as an important health care investment. However, existing preventive interventions have only shown modest effects. In order to improve the efficacy of prevention of depression and anxiety disorders, a number of authors have suggested that it is promising to focus on selective prevention programs that are offered to individuals scoring high on clearly established risk factors, whereby the preventive intervention then specifically targets these risk variables. This review presents repetitive negative thinking (worry and rumination) as a promising target for the prevention of depression and anxiety disorders.

抑郁症和焦虑症的预防被广泛认为是一项重要的卫生保健投资。然而,现有的预防性干预措施只显示出有限的效果。为了提高抑郁症和焦虑症的预防效果,许多作者建议,有希望将重点放在选择性预防项目上,这些项目提供给在明确确定的风险因素上得分较高的个人,从而预防干预专门针对这些风险变量。这篇综述提出了重复的消极思维(担心和反刍)作为预防抑郁和焦虑障碍的一个有希望的目标。
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引用次数: 81
The cultural theory and model of suicide 自杀的文化理论与模式
Joyce P. Chu, Peter Goldblum, Rebecca Floyd, Bruce Bongar

A growing body of research has demonstrated important variations in the prevalence, nature, and correlates of suicide across ethnic and sexual minority groups. Despite these developments, existing clinical and research approaches to suicide assessment and prevention have not incorporated cultural variations in any systematic way. In addition, theoretical models of suicide have been largely devoid of cultural influence. The current report presents a comprehensive analysis of literature describing the relationship between cultural factors and suicide in three major ethnic groups (African Americans, Asian Americans, and Latinos) and LGBTQ1 sexual minority groups. We utilized an inductive approach to synthesize this variegated body of research into four factors that account for 95% of existing culturally specific risk data: cultural sanctions, idioms of distress, minority stress, and social discord. These four cultural factors are then integrated into a theoretical framework: the Cultural Model of Suicide. Three theoretical principles emerge: (1) culture affects the types of stressors that lead to suicide; (2) cultural meanings associated with stressors and suicide affect the development of suicidal tendencies, one's threshold of tolerance for psychological pain, and subsequent suicidal acts; and (3) culture affects how suicidal thoughts, intent, plans, and attempts are expressed. The Cultural Model of Suicide provides an empirically guided cohesive approach that can inform culturally competent suicide assessment and prevention efforts in future research and clinical practice. Including both ethnic and sexual minorities in our investigations ensures advancement along a multiple identities perspective.

越来越多的研究表明,在种族和性少数群体中,自杀的流行程度、性质和相关性存在重要差异。尽管有这些发展,现有的自杀评估和预防的临床和研究方法并没有以任何系统的方式纳入文化差异。此外,自杀的理论模型在很大程度上缺乏文化影响。本报告综合分析了三个主要族群(非裔美国人、亚裔美国人和拉丁裔美国人)和LGBTQ1性少数群体中文化因素与自杀之间的关系。我们利用归纳方法将这一多样化的研究综合为四个因素,占现有文化特定风险数据的95%:文化制裁、痛苦习语、少数民族压力和社会不和谐。然后将这四个文化因素整合到一个理论框架中:自杀的文化模型。有三个理论原理:(1)文化影响导致自杀的压力源类型;(2)与压力源和自杀相关的文化意义影响自杀倾向的发展、心理痛苦耐受阈值和随后的自杀行为;(3)文化影响自杀想法、意图、计划和企图的表达方式。自杀的文化模型提供了一种经验指导的凝聚力方法,可以为未来的研究和临床实践中具有文化能力的自杀评估和预防工作提供信息。在我们的调查中包括少数民族和性少数群体,确保我们在多重身份的视角下取得进展。
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引用次数: 163
Reducing the stigma of mental disorders at work: A review of current workplace anti-stigma intervention programs 减少工作中精神障碍的耻辱感:对当前工作场所反耻辱感干预项目的回顾
Andrew C.H. Szeto , Keith S. Dobson

Stigma has been described as one of the largest barriers for those who have been diagnosed with a mental disorder, with negative consequences impacting all facets of life, including the workplace. Although many population-based anti-stigma initiatives exist, the need for workplace interventions is being recognized, particularly as the financial costs of mental disorders in the workplace mount. Specific workplace-focused programs are emerging to address this need. The present paper describes efforts to reduce the stigma related to mental disorders in the workplace. Following the review, suggestions are made for future workplace anti-stigma interventions, as well as a discussion of considerations for researchers who evaluate such programs.

污名被认为是精神障碍患者面临的最大障碍之一,会对生活的方方面面产生负面影响,包括工作场所。虽然存在许多以人群为基础的反污名倡议,但人们正在认识到工作场所干预措施的必要性,特别是在工作场所精神障碍的经济成本上升的情况下。专门针对工作场所的项目正在出现,以满足这一需求。本文描述了在工作场所减少与精神障碍有关的耻辱的努力。在回顾之后,对未来的工作场所反污名干预提出了建议,并讨论了评估此类计划的研究人员的考虑因素。
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引用次数: 80
Worry and the anxiety disorders: A meta-analytic synthesis of specificity to GAD 担忧和焦虑症:广泛性焦虑症特异性的综合meta分析
Bunmi O. Olatunji , Kate B. Wolitzky-Taylor , Craig N. Sawchuk , Bethany G. Ciesielski

Although worry is central to the diagnosis of generalized anxiety disorder (GAD), it is also commonly observed in other anxiety disorders. In this meta-analytic review, we empirically evaluated the extent to which worry is specific to GAD relative to patients with other anxiety disorders, those with other psychiatric disorders, and nonpsychiatric controls. A total of 47 published studies (N = 8,410) were included in the analysis. The results yielded a large effect size indicating greater severity/frequency of worry, meta-worry, and domains of worry among anxiety disorder patients v. nonpsychiatric controls (d = 1.64). In contrast to the many differences emerging from comparisons between anxiety disordered patients and nonpsychiatric controls, when anxiety disordered patients were compared to people with other psychiatric disorders they differed only on severity/frequency and not on meta-worry or domains of worry. A large effect size indicating greater severity/frequency of worry, meta-worry, and domains of worry among patients with GAD v. nonpsychiatric controls was also found (d = 2.05). However, differences between GAD and those with other psychiatric disorders also emerged for severity/frequency of worry. GAD was associated with greater worry difficulties than other anxiety disorders, which generally did not differ from those with other psychiatric disorders and each other. The implications of these findings for conceptualizing worry in GAD and other anxiety disorders, and the potentially moderating effects of age and gender are discussed.

虽然担忧是诊断广泛性焦虑症(GAD)的核心,但在其他焦虑症中也经常观察到。在这篇荟萃分析综述中,我们根据经验评估了相对于其他焦虑症患者、其他精神疾病患者和非精神疾病对照患者,焦虑对广泛性焦虑症的特异性程度。共有47篇已发表的研究(N = 8410)被纳入分析。结果产生了一个大的效应量,表明焦虑障碍患者比非精神病学对照组更严重/更频繁地担心、元担心和担心领域(d = 1.64)。与焦虑障碍患者和非精神疾病对照组之间的许多差异相比,当焦虑障碍患者与其他精神疾病患者进行比较时,他们只在严重程度/频率上存在差异,而在元担忧或担忧领域上没有差异。研究还发现,广泛性焦虑症患者与非精神病学对照组相比,担忧、元担忧和担忧领域的严重程度/频率更高(d = 2.05)。然而,广泛性焦虑症和其他精神疾病患者在担忧的严重程度/频率上也存在差异。与其他焦虑症相比,广泛性焦虑症与更大的担忧困难相关,这与其他精神疾病和彼此之间通常没有区别。本文讨论了这些发现对广泛性焦虑症和其他焦虑症中忧虑概念化的意义,以及年龄和性别的潜在调节作用。
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引用次数: 126
Author index (Volume 14 (2010)) 作者索引(第14卷(2010))
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引用次数: 0
The Army National Guard in OIF/OEF: Relationships among combat exposure, postdeployment stressors, social support, and risk behaviors OIF/OEF中的陆军国民警卫队:战斗暴露、部署后压力源、社会支持和风险行为之间的关系
James Griffith, Courtney West

With the continued operations in Iraq and Afghanistan, studies of the prevalence of posttraumatic stress disorder and related symptoms are now common. However, lacking is how these symptoms relate to precipitating conditions and the mitigating effects of social support on these symptoms. This is particularly relevant for reserve military personnel, who have been shown to be greater at-risk for postdeployment problems. The present study examined questionnaire data obtained from Army National Guard (ARNG) units immediately after their return from deployment to Iraq and Afghanistan during 2010 (N = 4329 soldiers in 50 units). Findings showed few soldiers displayed risk behaviors (i.e., daily alcohol use, use of illicit drugs, suicide thoughts, and physically threatening others) during and after deployment. Those most likely to have more postdeployment risk behaviors were also those who showed more risk behaviors during deployment. A substantial percentage of soldiers reported combat exposure, postdeployment negative emotions, and postdeployment loss of a personal relationship. These reported outcomes were all related to increased risk behaviors after deployment. The buffering effect of social support on postdeployment risk behaviors was equally evident when data were examined individually and when grouped by unit memberships. Implications of findings for future research, practice, and policies are discussed.

随着在伊拉克和阿富汗的持续行动,对创伤后应激障碍和相关症状的流行情况的研究现在很普遍。然而,缺乏的是这些症状如何与突发状况以及社会支持对这些症状的缓解作用有关。这对预备役军事人员尤其重要,他们在部署后出现问题的风险更大。本研究调查了2010年从部署到伊拉克和阿富汗的陆军国民警卫队(ARNG)部队返回后立即获得的问卷数据(N = 4329名士兵,50个单位)。调查结果显示,在部署期间和之后,很少有士兵表现出危险行为(即每天饮酒、使用非法药物、有自杀念头和对他人进行身体威胁)。那些最有可能有更多部署后风险行为的人,也是那些在部署期间表现出更多风险行为的人。相当大比例的士兵报告了战斗经历、部署后的负面情绪和部署后失去的个人关系。这些报告的结果都与部署后风险行为的增加有关。社会支持对部署后风险行为的缓冲作用在单独和按单位成员分组的数据中同样明显。讨论了研究结果对未来研究、实践和政策的影响。
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引用次数: 12
Subject index (Volume 14 (2010)) 主题索引(第14卷(2010))
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引用次数: 0
Moving the field of prevention from science to service: Integrating evidence-based preventive interventions into community practice through adapted and adaptive models 将预防领域从科学转向服务:通过适应和适应性模式将基于证据的预防干预措施纳入社区实践
Gerald J. August, Abigail Gewirtz, George M. Realmuto

The article addresses the adaptation of evidence-based prevention and positive youth development programs for community use. Two complementary approaches for adapting programs are described. In the “adapted” approach, programs are modified to accommodate the culture, climate, and operations of the organization delivering the program. In the “adaptive” approach, programs are modified to accommodate the characteristics, needs and preferences of the individual or family receiving the program. Two examples are provided that illustrate how both adapted and adaptive intervention strategies have been incorporated by community practitioners into the implementation of the Early Risers conduct problems prevention program.

这篇文章讨论了适应以证据为基础的预防和积极的青年发展计划,以供社区使用。描述了两种互补的适应方案的方法。在“适应”的方法中,程序被修改以适应文化、气候和交付程序的组织的操作。在“适应性”方法中,计划被修改以适应接受计划的个人或家庭的特征、需求和偏好。提供了两个例子来说明社区从业者如何将适应和适应性干预策略纳入到早起者行为问题预防计划的实施中。
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引用次数: 37
Searching for therapeutic change principles 寻找治疗改变原则
Bernard D. Beitman
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引用次数: 2
期刊
Applied & preventive psychology : journal of the American Association of Applied and Preventive Psychology
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