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Assessing Genetic Diversity in Bread Wheat (Triticum aestivum L.) using D2 Statistical Analysis 利用 D2 统计分析评估面包小麦(Triticum aestivum L. )的遗传多样性
Pub Date : 2024-01-29 DOI: 10.18805/ajdfr.dr-2139
B. A. Yasin, S. Shubhra, Misbahu Abdu, Garome Shiferaw, Dula Teshome, Gada Gudina, Legessu Girshe, Tesfaye Dugasa
Background: Genetic diversity is one of the potent components used to develop improved cultivars with a broad genetic base and wider adaptability. Genetic diversity between two parents of diverse origin generally displays greater heterosis and yields transgressive segregants. Therefore, knowledge of genetic diversity is the prerequisite for the genetic improvement of crop plants including wheat. D2 statistical analysis is one of the potent biometrical techniques used for the assessment of genetic diversity. Methods: The essence of the current experiment involved 40 providential bread wheat lines that were arranged and laid down in a randomized block design (RBD) with three replications at the experimental research farm of Suresh Gyan Vihar University, Jagatpura, Jaipur, during the rabi crop season (2020-21). The bread wheat lines of the same geographical range were assessed to determine the genetic diversity using D2 statistical analysis and heritability estimate in apropos to ten quantitative/qualitative characters. Result: Based on D2 analysis, the 40 promising wheat strains were lined up into ten different clusters to estimate the average distance at intra and inter-cluster levels. The uttermost intra-cluster distance was recorded in cluster X, whereas, the appreciative inter-cluster distance pertained among cluster-VI and cluster-X pursued by cluster-IX and cluster-X. Entrenched to the mean or average performance, most of the bread wheat lines were spotted to disclose appreciable distinctions concerning to their cluster means. It was perceived that the maximum percent contribution towards total divergence was exhibited by grain yield plant-1, followed by spike length, effective tiller plant-1, 1000 grain weight and plant height. Moreover, the number of effective tillers plant-1, spike length, harvest index ,1000 grain weight, number of grains spike-1 and protein content were quite substantial since these traits exhibit high heritability estimate together with high genetic advance as percent of mean.
背景:遗传多样性是用于培育具有广泛遗传基础和更强适应性的改良栽培品种的有力因素之一。来源不同的两个亲本之间的遗传多样性通常会表现出更大的异质性,并产生转基因分离株。因此,了解遗传多样性是包括小麦在内的农作物遗传改良的先决条件。D2 统计分析是用于评估遗传多样性的有效生物计量学技术之一。方法:本次实验的主要内容是在斋浦尔 Jagatpura 的 Suresh Gyan Vihar 大学的实验研究农场,于 2020-21 年小麦蕾季期间,以随机区组设计(RBD)的方式安排和布置了 40 个具有三个重复的天赐面包小麦品系。利用 D2 统计分析和遗传率估算,对同一地理范围内的面包小麦品系进行了评估,以确定十个定量/定性特征的遗传多样性。结果根据 D2 分析,将 40 个有潜力的小麦品系分成 10 个不同的聚类,以估算聚类内部和聚类之间的平均距离。簇内距离最大的是簇 X,而簇间距离最大的是簇 VI 和簇 X,其次是簇 IX 和簇 X。根据平均值或平均表现,大多数面包小麦品系的聚类平均值都有明显差异。结果表明,对总差异贡献最大的是植株-1 谷粒产量,其次是穗长、植株-1 有效分蘖数、千粒重和株高。此外,有效分蘖株数-1、穗长、收获指数、千粒重、穗粒数-1 和蛋白质含量也相当可观,因为这些性状表现出较高的遗传力估计值和较高的遗传进展(占平均值的百分比)。
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引用次数: 0
Use of Artichoke (Cynara scolymus) Flower Extract as a Substituteto Rennet in the Manufacture of Camel Milk Cheese 用朝鲜蓟(Cynara scolymus)花提取物替代雷尼特制造驼奶奶酪
Pub Date : 2024-01-24 DOI: 10.18805/ajdfr.drf-336
I. Fguiri, A. Sboui, M. Atigui, S. Arroum, M. Dbara, M. Hammadi, T. Khorchani
Background: The present study aims to improve coagulation abilities of camel milk using enzyme extracts from artichoke. Methods: The properties of purified extracts were studied in this research and a cheese was manufactured with artichoke extracts. Results: It showed an extraction yield of 38.83% and optimum coagulation conditions of the artichoke enzyme extract were: pH=5; temperature= 55°C; CaCl2 concentration= 0.01M and optimal enzyme concentration= 1%. A fresh cheese made from camel milk with a particular nutritional quality, consistency. The artichoke proteases displayed chymosin-like properties and thus hold the best potential for use as a milk coagulant in cheese production.
研究背景本研究旨在利用朝鲜蓟的酶提取物提高骆驼奶的凝结能力。研究方法:本研究对纯化提取物的特性进行了研究,并用朝鲜蓟提取物制造了一种奶酪。结果结果显示,提取率为 38.83%,朝鲜蓟酶提取物的最佳凝固条件为:pH=5;温度=55°C;CaCl2 浓度=0.01M;最佳酶浓度=1%。用骆驼奶制成的新鲜奶酪具有特殊的营养品质和稠度。朝鲜蓟蛋白酶具有类似糜蛋白酶的特性,因此最有可能用作奶酪生产中的牛奶凝固剂。
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引用次数: 0
The Influence of Major Milk Microbiota on the Characteristics of Milk and Milk Products: A Review 主要牛奶微生物群对牛奶和奶制品特性的影响:综述
Pub Date : 2024-01-19 DOI: 10.18805/ajdfr.dr-2144
Shivani Tomar, Vidyanand Tiwari
Milk is a nutrient-rich medium that encourages rapid microbial growth. The microorganisms associated with the milk of different milking animals and milk products have a significant impact on milk including milk from cows, buffalo, goats, and humans. Being nutritionally rich milk is a suitable growth medium for a variety of microorganisms and these have numerous positive and negative impacts on the properties of milk and their products. The high nutritional content of milk supports the growth of both beneficial and harmful microbiota. The entry of these microorganisms into the milk has various paths and sources, and, once in the milk, they start multiplication and increase their numbers. Many of these microbes show biological activities such as spore formation, toxin production, health promotion, biochemical alteration of medium, and other related activities. The authors are trying to present a detailed overview of the source, activity, and beneficial as well as harmful roles of microbes found in milk and milk products. The approach of authors to comprehensively review these topics is to examine the details of the microorganisms of milk to find out the activity and approach for improving milk processing and product stability for storage and sustainability in milk processing.
牛奶是一种营养丰富的介质,能促进微生物的快速生长。与不同挤奶动物的乳汁和乳制品相关的微生物对牛奶(包括牛奶、水牛、山羊和人)有重大影响。牛奶营养丰富,适合各种微生物生长,这些微生物对牛奶及其产品的特性有许多积极和消极的影响。牛奶的高营养成分有利于有益和有害微生物群的生长。这些微生物进入牛奶的途径和来源多种多样,一旦进入牛奶,它们就开始繁殖并增加数量。其中许多微生物具有生物活性,如孢子形成、毒素产生、促进健康、改变培养基的生化性质以及其他相关活动。作者试图详细介绍牛奶和奶制品中微生物的来源、活性、有益和有害作用。作者全面回顾这些主题的方法是研究牛奶中微生物的细节,找出其活性和方法,以改善牛奶加工和产品稳定性,实现牛奶加工的储存和可持续性。
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引用次数: 0
Identification of Methicillin-resistant Staphylococcus aureus Isolated from Dairy Cow’s Milk in Tulungagung District, Indonesia 印度尼西亚图伦加贡地区从牛奶中分离出的耐甲氧西林金黄色葡萄球菌的鉴定
Pub Date : 2024-01-18 DOI: 10.18805/ajdfr.drf-341
A. Khairullah, S. Sudjarwo, M. Effendi, S. Kurniawan, A. Widodo, O. Silaen, S. Ramandinianto
Background: Mastitis is one of the factors contributing to the health-related decreased milk production and quality for dairy cows. Mastitis in ruminants is a serious bacterial disease caused by Staphylococcus aureus. Staphylococcal bacteria are becoming increasingly resistant to many classes of antibiotics, particularly β-lactam families like the MRSA strain. Laboratory tests are required to determine the level of bacterial resistance and to identify MRSA isolates sourced from dairy cows in Tulungagung District. Methods: 110 milk samples were isolated on MSA media followed by Gram’s staining and biochemical tests. Kirby-bauer diffusion test-based assessment of antibiotic sensitivity. The S. aureus isolates that underwent the MRSA identification test were S. aureus isolates that had developed a resistance to β-lactam antibiotics. Result: A total 81 samples of the 110 isolated milk samples were determined to be positive for S. aureus. Out of total isolates, 25 isolates of S. aureus had the highest level of oxacillin resistance. As many as 4 isolates were confirmed to be MultiDrug Resistance (MDR) and 17 MRSA isolates were discovered from 100 samples of dairy cows. Early diagnosis of MRSA infection is crucial since it can be challenging to treat because this type of bacteria is known to be resistant to several drugs and spreads readily.
背景:乳腺炎是导致奶牛产奶量和牛奶质量下降的因素之一。反刍动物乳腺炎是由金黄色葡萄球菌引起的一种严重细菌性疾病。葡萄球菌对多种抗生素的耐药性越来越强,尤其是对β-内酰胺类抗生素,如 MRSA 菌株。需要进行实验室检测,以确定细菌的耐药性水平,并识别来自图伦加贡地区奶牛的 MRSA 分离物。方法:在 MSA 培养基上分离 110 份牛奶样本,然后进行革兰氏染色和生化测试。基于柯比鲍尔扩散试验评估抗生素敏感性。接受 MRSA 鉴定测试的金黄色葡萄球菌分离物是对β-内酰胺类抗生素产生耐药性的金黄色葡萄球菌分离物。结果在 110 个分离出的牛奶样本中,共有 81 个样本被确定为金黄色葡萄球菌阳性。在所有分离菌中,25 个分离菌对奥沙西林的耐药性最高。多达 4 个分离菌株被证实具有多重耐药性(MDR),从 100 个奶牛样本中发现了 17 个 MRSA 分离菌株。早期诊断 MRSA 感染至关重要,因为这类细菌已知对多种药物具有耐药性,而且很容易传播,因此治疗具有挑战性。
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引用次数: 0
Study of Milk Cost Production in Buffaloes of Telangana State 特兰甘纳邦水牛牛奶成本生产研究
Pub Date : 2024-01-17 DOI: 10.18805/ajdfr.dr-2154
Y. S. Babu, A. S. Chandra, R.M.V. Prasad, D. Nagalakshmi, M. S. Reddy
Background: Telangana state stands 13th position in milk production with 5.61 MT of milk production during 2019-20 as compared to 4.2 MT in 2014-15. The profitability of dairy enterprise is determined by sale price of milk and of late there is lot of uproar from farming community on lack of proper support price for milk. So having adequate data on cost of milk production in different sized farms in buffaloes will enable policy makers to arrive at a minimum support price for milk, so that the farmer will not be put to loss. Methods: An investigation was carried out to study the cost of milk production in buffaloes of dairy farmers in Karimnagar, Khammam and Rangareddy districts of Telangana. The data was collected from 240 farmers spread over 24 villages in 6 mandals in the selected districts. The 240 respondents were interviewed as per the questionnaire. Result: Average milk production per day per animal, sale price of milk per kg per animal per day was 8.63 lit, Rs.35.42 respectively. Cost of milk production in large, medium and small herd size was found to be Rs.26.32, Rs.26.68, Rs.27.08, respectively. Average cost of milk production per animal per day was found to be Rs.26.49. The average net income from cow was Rs.77.03. The average profit/litre of milk was calculated as Rs.8.92/lit. The average benefit-cost ratio was found to be 1.33.
背景:特兰甘纳邦的牛奶产量位居第 13 位,2019-20 年度为 5.61 公吨,而 2014-15 年度为 4.2 公吨。奶制品企业的盈利能力取决于牛奶的销售价格,而最近农业社区对缺乏适当的牛奶支持价格提出了很多质疑。因此,掌握不同规模水牛养殖场的牛奶生产成本的充分数据,将有助于政策制定者确定最低牛奶支持价格,从而避免奶农蒙受损失。方法:对 Telangana 的 Karimnagar、Khammam 和 Rangareddy 地区奶农的水牛产奶成本进行了调查。数据是从所选地区 6 个县 24 个村庄的 240 名奶农中收集的。根据调查问卷对 240 名受访者进行了访谈。结果每头牲畜每天的平均产奶量和每公斤牲畜每天的牛奶销售价格分别为 8.63 升和 35.42 卢比。大型、中型和小型牛群的牛奶生产成本分别为 26.32 卢比、26.68 卢比和 27.08 卢比。每头奶牛每天的平均产奶成本为 26.49 卢比。奶牛的平均净收入为 77.03 卢比。每升牛奶的平均利润为 8.92 卢比。平均效益成本比为 1.33。
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引用次数: 0
Inclusion of Sorghum in Ruminant Diets Towards Methane Emission Mitigation and Improved Meat Quality: A Review 在反刍动物日粮中添加高粱以减少甲烷排放并提高肉质:综述
Pub Date : 2024-01-15 DOI: 10.18805/ajdfr.drf-333
Nyasha Rugwete, T. Muti̇bvu, T. Halimani
Sorghum is among the less widely used crops yet it is ranked the fifth most important cereal crop after wheat, maize, rice and barley. The grain’s nutritional value is similar to that of maize and wheat and the phenolic profile is more abundant and varied than other common cereal grains. Sorghum has been largely included in ruminant diets as an energy source and research findings show insignificant differences in the performance parameters in livestock fed sorghum-based diets, with some studies suggesting sorghum as a more efficient alternative to maize. The narrative review focuses on opportunities for reducing rumen methanogenesis through dietary inclusion of sorghum as well as discussing the impact on relative abundance of microbiota and meat quality. The grain possesses qualities that can potentially influence the reduction of greenhouse gas emissions in ruminant livestock production, relative abundance of rumen microbes and meat quality. However, there is very little information on the influence of sorghum grain-based diets on the rumen bacterial community composition and their relationship with ruminal metabolites. Further investigations are required to add more knowledge on the effect of sorghum grain-based diets on meat quality.
高粱是使用较少的农作物之一,但却被列为继小麦、玉米、大米和大麦之后的第五大重要谷物作物。高粱谷物的营养价值与玉米和小麦相似,而酚类物质则比其他常见谷物更为丰富多样。研究结果表明,以高粱为基础的日粮在牲畜的性能参数方面差异不大,一些研究表明高粱是玉米的更有效替代品。这篇综述重点讨论了通过在日粮中添加高粱来减少瘤胃产甲烷的机会,以及高粱对微生物群相对丰度和肉质的影响。高粱谷物的品质有可能影响反刍家畜生产中温室气体的减排、瘤胃微生物的相对丰度和肉质。然而,关于以高粱谷物为基础的日粮对瘤胃细菌群落组成的影响及其与瘤胃代谢物之间关系的信息却很少。要进一步了解高粱谷物日粮对肉质的影响,还需要进一步的研究。
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引用次数: 0
Optimization and Quality Characteristics of Mango Peel Pectin 芒果皮果胶的优化和质量特性
Pub Date : 2024-01-08 DOI: 10.18805/ajdfr.dr-2019
K.R. Naveena, Geetha M. Yankanchi, Shilpa Yatnatti, K. Geetha
Background: Pectin’s are complex carbohydrate molecules mainly used as gelling agents, thickener, emulsifier and stabilizer in the food and beverage industry. Methods: Pectin was extracted from mango peel powder by acid extraction method with different combinations of precipitation time and sample to solution ratio to optimize highest yield and its quality characteristics were studied in comparison with commercial pectin (CP). Result: The maximum yield (14.23%) was obtained for C-9 combination. Functional properties such as solubility, swelling index, water absorption and bulk density ranged from 49.74 to 72.27 per cent, 2.89 to 5.22 g/g, 3.21 to 6.82 ml/g and 1.06 to 1.59 g/cm3. In commercial pectin it was 65.99±0.63 per cent, 2.65±0.14 g/g, 5.79±0.15 ml/g and 1.29±0.07 g/cm3 respectively. Quality characteristics such as Degree of esterification, equivalent weight, methoxyl content and total anhydrouronic acid ranged from 83.37 to 88.98 per cent, 960.5 to 831, 3.83 to 5.28 per cent and 52.30 to 40.5 respectively. In commercial pectin it was 89.03, 906.5, 4.07 and 42.67 per cent respectively. Findings revealed that pectin extracted from mango peel possess high chances to be exploited and commercialized as most of the functional properties and quality characteristics are on par with commercial pectin.
背景:果胶是一种复杂的碳水化合物分子,在食品和饮料行业中主要用作胶凝剂、增稠剂、乳化剂和稳定剂。研究方法采用酸萃取法从芒果果皮粉中提取果胶,通过不同的沉淀时间和样品与溶液比例组合来优化最高产量,并将其质量特性与商用果胶(CP)进行比较研究。结果C-9 组合的产率最高(14.23%)。功能特性如溶解度、膨胀指数、吸水率和体积密度分别为 49.74%至 72.27%、2.89 至 5.22 克/克、3.21 至 6.82 毫升/克和 1.06 至 1.59 克/立方厘米。商品果胶的含量分别为 65.99±0.63%、2.65±0.14 克/克、5.79±0.15 毫升/克和 1.29±0.07 克/立方厘米。酯化度、当量重量、甲氧基含量和总无水酸等质量特性的范围分别为 83.37%至 88.98%、960.5%至 831%、3.83%至 5.28%和 52.30%至 40.5%。商品果胶的含量分别为 89.03%、906.5%、4.07% 和 42.67%。研究结果表明,从芒果皮中提取的果胶具有很高的开发和商业化机会,因为它的大多数功能特性和质量特征与商业果胶相当。
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引用次数: 0
Evolution of Some Biochemical Parameters during Cold Storage and Their Effect on the Formation of Acrylamide in Fried Potatoes 冷藏期间一些生化参数的变化及其对炸薯条中丙烯酰胺形成的影响
Pub Date : 2024-01-02 DOI: 10.18805/ajdfr.drf-348
A. Abed, Z. Kaci, T. Dahmane, N. Tirchi, D. B. Cherif
Background: The province of Ain Defla (Algeria) is known for its high potato production potential. However, many challenges need to be overcome, among them the deterioration of biochemical and organoleptic quality after harvest and during storage of potato tubers. Methods: A study was carried out in this context over two successive years 2019 and 2020, with the aim of determining the degree of physiological evolution of tubers of four potato varieties (Kondor, Bartina, Spunta and Atlas) through biochemical indicators, such as pH, acidity, dry matter, reducing sugars and starch, during cold storage at a temperature of 6°C±2 and for 60 days and 90 days. A qualitative analysis of acrylamide formation under the effect of temperature and frying time was carried out. Result: Kondor revealed the highest pH value before and during storage. A progressive decrease in dry matter and starch as a function of storage time was detected for all varieties studied, notably Spunta and Atlas. The Spunta variety showed significant amounts of reducing sugars during 60 days of storage. Acrylamide formation was intense at 160°C for 4 minutes of frying in both Spunta and Kondor varieties. However, Kondor showed greater resistance to enzymatic browning (acrylamide) than Spunta under the same empirical conditions.
背景:艾因德弗拉省(阿尔及利亚)以马铃薯生产潜力大而闻名。然而,需要克服许多挑战,其中包括马铃薯块茎收获后和储存期间的生化和感官质量下降。方法:在此背景下,于 2019 年和 2020 年连续两年开展了一项研究,旨在通过 pH 值、酸度、干物质、还原糖和淀粉等生化指标,确定四个马铃薯品种(Kondor、Bartina、Spunta 和 Atlas)块茎在 6°C±2 的温度下冷藏 60 天和 90 天期间的生理变化程度。在温度和油炸时间的影响下,对丙烯酰胺的形成进行了定性分析。结果如下Kondor 在贮藏前和贮藏期间的 pH 值最高。所研究的所有品种,特别是 Spunta 和 Atlas,都发现干物质和淀粉随着贮藏时间的延长而逐渐减少。Spunta 品种在 60 天的储藏过程中显示出大量的还原糖。Spunta 和 Kondor 两个品种在 160 摄氏度油炸 4 分钟时丙烯酰胺的形成都很强烈。不过,在相同的经验条件下,Kondor 比 Spunta 对酶促褐变(丙烯酰胺)的抵抗力更强。
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引用次数: 0
The Intervention of Anchovy (Stokphorus commrsouli) Calcium Extract Could Elevate Calcium Levels during Pregnancy and Give Birth and Promote Bone Development: An in vivo Study 鳀鱼(Stokphorus commrsouli)钙提取物的干预可提高妊娠期和分娩期的钙水平并促进骨骼发育:体内研究
Pub Date : 2024-01-02 DOI: 10.18805/ajdfr.drf-306
Supriyana, Irma HY Siregar, Lanny Sunarjo, Endah Aryati, Dina Fatmawati
Background: During pregnancy, calcium deficiency has become a frequent phenomenon. Calcium intake is essential for supporting maternal and fetal health to maintain calcium homeostasis and promotes bone formation. Anchovy is a high calcium source from marine. The anchovy diet impact on fetal development during pregnancy is inconclusive. Therefore, Anchovy Calcium Extract (ACE) effect on calcium homeostasis and bone formation was studied. Methods: Female Sprague Dawley rats were grouped into three: without ACE administration, with ACE administration prior to mating until two weeks post-labor and with ACE administration after pregnancy confirmation until two weeks post-labor. Blood calcium levels and bone hardness were measured. Result: ACE improved maternal’s and offspring’s calcium levels. ACE also provided adequate blood calcium levels during pregnancy, labor and breastfeeding. In addition, ACE improve maternal bone firmness but has no significant effect on its offspring. In conclusion, ACE has excellent potential as a dietary supplement to promote adequate calcium requirements for maternal and infant health.
背景:孕期缺钙已成为一种常见现象。钙的摄入对支持母体和胎儿健康、维持钙平衡和促进骨骼形成至关重要。鯷鱼是来自海洋的高钙来源。鳀鱼饮食对孕期胎儿发育的影响尚无定论。因此,我们研究了凤尾鱼钙提取物(ACE)对钙平衡和骨骼形成的影响。研究方法将雌性 Sprague Dawley 大鼠分为三组:未服用 ACE 大鼠组、交配前服用 ACE 大鼠组直至产后两周组和确认妊娠后服用 ACE 大鼠组直至产后两周组。测量血钙水平和骨质硬度。结果ACE 提高了母体和后代的血钙水平。在怀孕、分娩和哺乳期间,ACE 还能提供充足的血钙水平。此外,ACE 还能改善母体的骨骼坚硬度,但对子代没有明显影响。总之,作为一种膳食补充剂,ACE 具有促进母婴健康所需的充足钙质的巨大潜力。
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引用次数: 0
Storage and Packaging Material Effects on Pomegranate (Punica granatum) Arils Quality Attributes during Storage 贮藏和包装材料对石榴(Punica granatum)果仁贮藏期间质量属性的影响
Pub Date : 2024-01-01 DOI: 10.18805/ajdfr.dr-2074
V. Dhineshkumar, C. Vigneshwaran
Background: Minimally processed ready-to-eat pomegranate arils have become popular due to their convenience, high value, unique sensory characteristics and health benefits. The main objective of this key research is to understand the effect of packaging materials and the quality profile of pomegranate arils (Bhagwa) storage at 5-7°C, 85-90% RH till 18 days was tested. Methods: Minimally processed arils at three-day intervals throughout storage, the antioxidant and phytochemical characteristics were assessed. There has been a variable change in the ascorbic acid concentration level, phenolic, antioxidant and anthocyanin content due to the packaging material with minimum processed arils in sensory analysis. Result: When the pomegranate Arils packed in breathable film indicated high retention of phenols antioxidants, anthocyanins, ascorbic acid and total than arils packed in LDPE or PP. Breathable film shown better quality attributes for minimally processed arils over an 18-day storage period as compared to other packaging materials.
背景:微加工即食石榴果仁因其方便、高价值、独特的感官特征和健康益处而广受欢迎。这项关键研究的主要目的是了解包装材料的影响,并测试石榴果仁(Bhagwa)在 5-7°C 和 85-90% 相对湿度条件下贮藏 18 天的质量状况。方法在整个贮藏过程中,每隔三天对石榴果实进行微量加工,评估其抗氧化剂和植物化学特性。在感官分析中,抗坏血酸浓度水平、酚类物质、抗氧化剂和花青素含量都会因最小加工假种皮的包装材料而发生变化。结果用透气薄膜包装的石榴果实比用低密度聚乙烯或聚丙烯包装的石榴果实保留了更多的酚类抗氧化剂、花青素、抗坏血酸和总含量。与其他包装材料相比,透气薄膜在 18 天的贮藏期内显示出更佳的质量属性。
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引用次数: 0
期刊
Asian Journal of Dairy and Food Research
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