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Neuroinvasive West Nile Virus Infections in the Trakya Region of Türkiye. 土耳其特拉基亚地区的西尼罗河病毒感染。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 DOI: 10.4274/balkanmedj.galenos.2024.2024-9-72
Habibe Tülin Elmaslar Mert, Zerrin Yuluğkural, Ezgi Kula, Betül Yüzügüldü, Figen Kuloğlu
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引用次数: 0
Exploring the Effect of Fidgetin-Like 1 on Colorectal Cancer Through Tissue Chip and In Vitro Experiments 通过组织芯片和体外实验探索 Fidgetin-Like 1 对结直肠癌的影响
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-09-25 DOI: 10.4274/balkanmedj.galenos.2024.2024-7-9
Yunxing Xu, Yucheng Shen, Chen Zhang, Liangfeng Zheng, Feiyue Ji, Jin Chen, Shouliang Cheng, Yu Zheng

Background: Fidgetin-like 1 (FIGNL1) is extensively overexpressed in a variety of cancers. It facilitates non‑small cell lung cancer tumor cell proliferation and hepatocellular carcinoma formation due to abnormal DNA repair. Clinically relevant data indicates that its high expression is linked with the poor prognosis of patients with renal clear-cell carcinoma, low-grade gliomas, and hepatocellular carcinoma. Nevertheless, the scope of FIGNL1’s involvement in cancer, particularly colorectal cancer (CRC), remains unclear.

Aims: To investigate the function of FIGNL1 in CRC.

Study design: Cell culture study.

Methods: The TCGA database and immunohistochemistry analysis were employed to investigate FIGNL1 expression in CRC tissue. A cell viability assay was performed using the Cell Counting Kit-8. The cell migration and invasion were evaluated using the transwell assay. Small interfering RNA (siRNA) transfection was conducted to knockdown FIGNL1 expression. Infection with FIGNL1 overexpression lentivirus was performed to promote FIGNL1 overexpression. The STRING database was employed for predicting protein interaction.

Results: FIGNL1 was substantially upregulated in human CRC tissues and was associated with TNM stages and lymph node metastasis in patients. The inhibition of CRC cell proliferation, migration, and invasion in Caco-2 cells was achieved by silencing FIGNL1 using siRNA. Additional investigations suggested that FIGNL1 overexpression could promote CRC cell proliferation, migration, and invasion via P38 signaling pathway activation in Colo-205 cells. Subsequent experiments demonstrated that FIGNL1-mediated P38 phosphorylation was contingent upon SPIDR interaction.

Conclusion: These results implied that FIGNL1 was a potential anticancer drug target, which also offered a novel strategy for future CRC treatment.

背景:Fidgetin-like 1(FIGNL1)在多种癌症中广泛过表达。由于 DNA 修复异常,它有助于非小细胞肺癌肿瘤细胞的增殖和肝细胞癌的形成。临床相关数据表明,它的高表达与肾透明细胞癌、低级别胶质瘤和肝细胞癌患者的不良预后有关。目的:研究 FIGNL1 在 CRC 中的功能:方法:利用 TCGA 数据库和免疫组化分析研究 FIGNL1 在 CRC 组织中的表达。使用细胞计数试剂盒-8进行细胞活力检测。细胞迁移和侵袭的评估采用透孔试验。通过小干扰 RNA(siRNA)转染来敲除 FIGNL1 的表达。用FIGNL1过表达慢病毒感染以促进FIGNL1的过表达。采用 STRING 数据库预测蛋白质相互作用:结果:FIGNL1在人类CRC组织中大量上调,并与患者的TNM分期和淋巴结转移有关。使用 siRNA 沉默 FIGNL1 可抑制 Caco-2 细胞中 CRC 细胞的增殖、迁移和侵袭。其他研究表明,在Colo-205细胞中,FIGNL1的过表达可通过激活P38信号通路促进CRC细胞的增殖、迁移和侵袭。随后的实验证明,FIGNL1 介导的 P38 磷酸化取决于 SPIDR 的相互作用:这些结果表明,FIGNL1 是一个潜在的抗癌药物靶点,这也为未来治疗 CRC 提供了一种新策略。
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引用次数: 0
Takotsubo Syndrome: An International Expert Consensus Report on Practical Challenges and Specific Conditions (Part-2: Specific Entities, Risk Stratification and Challenges After Recovery) 塔克氏综合征:关于实际挑战和特定情况的国际专家共识报告(第二部分:特定实体、风险分层和康复后的挑战)。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-10-17 DOI: 10.4274/balkanmedj.galenos.2024.2024-9-99
Kenan Yalta, John E Madias, Nicholas G Kounis, Shams Y-Hassan, Marija Polovina, Servet Altay, Alexandre Mebazaa, Mehmet Birhan Yilmaz, Yuri Lopatin, Mamas A Mamas, Robert J Gil, Ritu Thamman, Abdallah Almaghraby, Biykem Bozkurt, Gani Bajraktari, Thomas Fink, Vassil Traykov, Stephane Manzo-Silberman, Ulvi Mirzoyev, Sekib Sokolovic, Zviad V Kipiani, Cecilia Linde, Petar M Seferovic

Takotsubo syndrome (TTS) still remains as an enigmatic phenomenon. In particular, long-term challenges (including clinical recurrence and persistent symptoms) and specific entities in the setting of TTS have been the evolving areas of interest. On the other hand, a significant gap still exists regarding the proper risk-stratification of this phenomenon in the short and long terms. The present paper, the second part (part-2) of the consensus report, aims to discuss less well-known aspects of TTS including specific entities, challenges after recovery and risk-stratification.

高骤变综合征(TTS)仍然是一个神秘的现象。特别是,TTS 的长期挑战(包括临床复发和症状持续存在)和特定实体一直是人们不断关注的领域。另一方面,在对这一现象进行适当的短期和长期风险分级方面仍存在巨大差距。本文是共识报告的第二部分(第二部分),旨在讨论 TTS 鲜为人知的方面,包括特定实体、康复后的挑战和风险分级。
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引用次数: 0
Integration of Artificial Intelligence and Genome Editing System for Determining the Treatment of Genetic Disorders 整合人工智能和基因组编辑系统,确定遗传疾病的治疗方法。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-08-16 DOI: 10.4274/balkanmedj.galenos.2024.2024-080824
Suat Erdoğan
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引用次数: 0
Comment on “Exploring Regional Disparities in Heart Failure Epidemiology and Outcomes: A Comprehensive Study Across Geographical Regions in Türkiye” 关于 "探索心力衰竭流行病学和结果的地区差异:图尔基耶跨地域综合研究 "发表评论。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-08-28 DOI: 10.4274/balkanmedj.galenos.2024.2024-7-96
Levent Pay
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引用次数: 0
Chronic Nasal Administration of Kisspeptin-54 Regulates Mood-Related Disorders Via Amygdaloid GABA in Hemi-Parkinsonian Rats 慢性鼻腔给药 Kisspeptin-54 通过杏仁核 GABA 调节半帕金森大鼠的情绪相关障碍
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-09-25 DOI: 10.4274/balkanmedj.galenos.2024.2024-7-46
Osman Sinen, Ayşegül Gemici Sinen, Narin Derin, Mutay Aydın Aslan

Background: Depression and anxiety, the most prevalent neuropsychiatric manifestations in Parkinson’s disease (PD), negatively impact their quality of life.

Aims: To determine whether the chronic nasal administration of kisspeptin-54 (KP-54) could. Alleviate symptoms of anxiety and depression in hemi-Parkinsonian rats.

Study design: Experimental study.

Methods: This study included adult Sprague Dawley male rats who were administered either a vehicle (artificial cerebrospinal fluid) or 6-hydroxydopamine (6-OHDA) unilaterally into the medial forebrain bundle. The vehicle, or KP-54 (3 nmol/kg, applied topically to the rhinarium), was administered daily for a seven-day period. The sucrose preference test (SPT), elevated plus maze test (EPMT), and open field test (OFT) were implemented to evaluate depression- and anxiety-like behaviors, respectively, seven days following the lesion surgery. Gamma-aminobutyric acid (GABA) concentrations in the amygdala were quantified using mass spectrometry. Tyrosine hydroxylase in substantia nigra was analyzed using immunohistochemistry.

Results: The nasal delivery of KP-54 significantly reduced depressionand anxiety-like behaviors that were induced by 6-OHDA, as indicated by the results of the SPT, OFT, and EPMT. Moreover, it was observed that nasal KP-54 effectively mitigated 6-OHDA-induced motor deficits and the loss of nigral dopaminergic neurons. The nasal administration of KP-54 augmented the decline in GABA levels in the amygdala induced by 6-OHDA. Furthermore, effective correlations were established between GABA concentrations and behavioral parameters.

Conclusion: The nasal delivery of KP-54 could function as a viable therapeutic alternative for treating mood-related disorders in PD.

背景:抑郁和焦虑是帕金森病(PD)最普遍的神经精神表现,对患者的生活质量有负面影响。研究目的:确定长期鼻腔给药kisspeptin-54(KP-54)能否缓解半帕金森大鼠的焦虑和抑郁症状:实验研究:研究对象:成年 Sprague Dawley 雄性大鼠,单侧前脑内侧束注射载体(人工脑脊液)或 6-羟基多巴胺(6-OHDA)。在为期七天的时间里,每天给大鼠注射载体或 KP-54(3 nmol/kg,鼻腔局部注射)。蔗糖偏好试验(SPT)、高架迷宫试验(EPMT)和开阔地试验(OFT)分别用于评估病变手术七天后的抑郁和焦虑行为。杏仁核中的γ-氨基丁酸(GABA)浓度采用质谱法进行量化。用免疫组化方法分析了黑质中的酪氨酸羟化酶:结果:从SPT、OFT和EPMT的结果来看,鼻腔给药KP-54能显著减少6-OHDA诱导的抑郁和焦虑行为。此外,还观察到鼻腔给药 KP-54 能有效缓解 6-OHDA 诱导的运动障碍和黑质多巴胺能神经元的缺失。鼻腔给药 KP- 54 可增强 6-OHDA 引起的杏仁核 GABA 水平下降。此外,GABA浓度与行为参数之间建立了有效的相关性:结论:KP-54的鼻腔给药可作为治疗帕金森病情绪相关障碍的一种可行的替代疗法。
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引用次数: 0
Congenital Hypofibrinogenemia is a Potential Risk Factor for Ischemic Stroke 先天性低纤维蛋白原血症是缺血性中风的潜在风险因素。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-08-16 DOI: 10.4274/balkanmedj.galenos.2024.2024-6-9
Demet Bölükbaşı, Şerife Gökbulut Bektaş, Duygu Kayar Çalılı, Şeval İzdeş
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引用次数: 0
The Mpox 2024 Outbreak: The Main Challenges. Mpox 2024 爆发:主要挑战。
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 DOI: 10.4274/balkanmedj.galenos.2024.2024-240924
Şiran Keske, Hüseyin Güdücüoğlu, Önder Ergönül
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引用次数: 0
Tenosynovial Giant Cell Tumor in the Gruberi Bursa 格鲁贝里滑囊腱鞘巨细胞瘤:位置独特的罕见实体瘤
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-08-15 DOI: 10.4274/balkanmedj.galenos.2024.2024-4-120
Fábio Ferreira, Diana Baptista, Miguel Castro
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引用次数: 0
Mesencephalic Developmental Venous Anomaly Causing Obstructive Hydrocephalus Due to Aqueductal Stenosis 间脑发育性静脉异常导致导水管狭窄引起阻塞性脑积水
IF 1.9 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-10-31 Epub Date: 2024-08-26 DOI: 10.4274/balkanmedj.galenos.2024.2024-7-37
Gökhan Aytekin, Merve Tuna, Serdar Solak
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引用次数: 0
期刊
Balkan Medical Journal
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