Pub Date : 2019-11-06eCollection Date: 2019-01-01DOI: 10.1155/2019/7067619
Yang Feng, Ruixia Cui, Zeyu Li, Xia Zhang, Yifan Jia, Xing Zhang, Jinghong Shi, Kai Qu, Chang Liu, Jingyao Zhang
Acetaminophen- (APAP-) induced hepatic injury is an important clinical challenge. Oxidative stress, inflammation, apoptosis, and endoplasmic reticulum stress (ERS) contribute to the pathogenesis. Methane has potential anti-inflammatory, antioxidant, and antiapoptotic properties. This project was aimed at studying the protective effects and relative mechanisms of methane in APAP-induced liver injury. In the in vivo experiment, C57BL/6 mice were treated with APAP (400 mg/kg) to induce hepatic injury followed by methane-rich saline (MRS) 10 ml/kg i.p. after 12 and 24 h. We observed that MRS alleviated the histopathological lesions in the liver, decreased serum aminotransferase levels, reduced the levels of inflammatory cytokines, suppressed the nuclear factor-κB expression. Further, we found that MRS relieved oxidative stress by regulating the Nrf2/HO-1/NQO1 signaling pathway and their downstream products after APAP challenge. MRS also regulated proteins associated with ERS-induced apoptosis. In the in vitro experiment, the L-02 cell line was treated with APAP (10 mM) to induce hepatic injury. We found that a methane-rich medium decreased the levels of reactive oxygen species (DHE fluorescent staining), inhibited apoptosis (cell flow test), and regulated the Nrf2/HO-1/NQO1 signaling pathway. Our data indicated that MRS prevented APAP-induced hepatic injury via anti-inflammatory, antioxidant, anti-ERS, and antiapoptotic properties involving the Nrf2/HO-1/NQO1 signaling pathway.
{"title":"Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway.","authors":"Yang Feng, Ruixia Cui, Zeyu Li, Xia Zhang, Yifan Jia, Xing Zhang, Jinghong Shi, Kai Qu, Chang Liu, Jingyao Zhang","doi":"10.1155/2019/7067619","DOIUrl":"10.1155/2019/7067619","url":null,"abstract":"<p><p>Acetaminophen- (APAP-) induced hepatic injury is an important clinical challenge. Oxidative stress, inflammation, apoptosis, and endoplasmic reticulum stress (ERS) contribute to the pathogenesis. Methane has potential anti-inflammatory, antioxidant, and antiapoptotic properties. This project was aimed at studying the protective effects and relative mechanisms of methane in APAP-induced liver injury. In the <i>in vivo</i> experiment, C57BL/6 mice were treated with APAP (400 mg/kg) to induce hepatic injury followed by methane-rich saline (MRS) 10 ml/kg i.p. after 12 and 24 h. We observed that MRS alleviated the histopathological lesions in the liver, decreased serum aminotransferase levels, reduced the levels of inflammatory cytokines, suppressed the nuclear factor-<i>κ</i>B expression. Further, we found that MRS relieved oxidative stress by regulating the Nrf2/HO-1/NQO1 signaling pathway and their downstream products after APAP challenge. MRS also regulated proteins associated with ERS-induced apoptosis. In the <i>in vitro</i> experiment, the L-02 cell line was treated with APAP (10 mM) to induce hepatic injury. We found that a methane-rich medium decreased the levels of reactive oxygen species (DHE fluorescent staining), inhibited apoptosis (cell flow test), and regulated the Nrf2/HO-1/NQO1 signaling pathway. Our data indicated that MRS prevented APAP-induced hepatic injury via anti-inflammatory, antioxidant, anti-ERS, and antiapoptotic properties involving the Nrf2/HO-1/NQO1 signaling pathway.</p>","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":"35 1","pages":"7067619"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85546524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How China Plans To Inhabit Its Future","authors":"R. Koolhaas","doi":"10.1111/npqu.12229","DOIUrl":"https://doi.org/10.1111/npqu.12229","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/npqu.12229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45086652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mass Urban Migration Is The Epic Story of Our Time","authors":"Orhan Pamuk","doi":"10.1111/npqu.12227","DOIUrl":"https://doi.org/10.1111/npqu.12227","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/npqu.12227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48592199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.1111/j.1540-5842.2010.01144.x
Orhan Pamuk
{"title":"Caressing The World With Words","authors":"Orhan Pamuk","doi":"10.1111/j.1540-5842.2010.01144.x","DOIUrl":"https://doi.org/10.1111/j.1540-5842.2010.01144.x","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1540-5842.2010.01144.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44192794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bill Gates’ Guru: “I'm Not Impressed With Silicon Valley.” “I Don't Have a Cell Phone.” “I Never Blog.”","authors":"V. Smil","doi":"10.1111/npqu.12231","DOIUrl":"https://doi.org/10.1111/npqu.12231","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/npqu.12231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49023393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The World Is Returning To Pluralism After American Hegemony","authors":"P. Sloterdijk","doi":"10.1111/npqu.12225","DOIUrl":"https://doi.org/10.1111/npqu.12225","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/npqu.12225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46185654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Human History Will End When Men Become Gods","authors":"Y. Harari","doi":"10.1111/npqu.12223","DOIUrl":"https://doi.org/10.1111/npqu.12223","url":null,"abstract":"","PeriodicalId":87197,"journal":{"name":"New perspectives quarterly : NPQ","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/npqu.12223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41471744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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