Pub Date : 2023-11-28DOI: 10.1007/s13206-023-00127-x
Yejin Yoon, Yein Kwon, Hanbin Park, Siyun Lee, Chulhwan Park, Taek Lee
The coronavirus disease pandemic has led to an urgent need for rapid and accurate viral diagnosis. Therefore, rapid biosensors, not only for viruses but also for the detection of bacteria, disease diagnosis, and environmental monitoring, have been actively researched. Biosensors analyze the binding of biomolecules and target substances mainly based on electrochemical, electrical, or optical methods. To achieve precise and rapid diagnosis, it is crucial to reduce the time required for biomolecule–target substance binding. Typically, biomolecules reach the target substances through random diffusion, and to overcome the limitations associated herewith, biosensors have been integrated with alternating current (AC) electrokinetics (ACEK) technology. ACEK, through the application of alternating voltages, converts electrical energy into fluid motion, inducing pumping, mixing, concentration, and separation of the fluid. Its low power consumption makes it highly promising as a point-of-care diagnostic device. In this paper, we review the advancements in three ACEK technologies: AC electrothermal flow, AC electro-osmosis, and AC di-electrophoresis, to discuss the development of rapid biosensor fabrication methods based on electrical potential applications.
{"title":"Recent Progress in Rapid Biosensor Fabrication Methods: Focus on Electrical Potential Application","authors":"Yejin Yoon, Yein Kwon, Hanbin Park, Siyun Lee, Chulhwan Park, Taek Lee","doi":"10.1007/s13206-023-00127-x","DOIUrl":"https://doi.org/10.1007/s13206-023-00127-x","url":null,"abstract":"<p>The coronavirus disease pandemic has led to an urgent need for rapid and accurate viral diagnosis. Therefore, rapid biosensors, not only for viruses but also for the detection of bacteria, disease diagnosis, and environmental monitoring, have been actively researched. Biosensors analyze the binding of biomolecules and target substances mainly based on electrochemical, electrical, or optical methods. To achieve precise and rapid diagnosis, it is crucial to reduce the time required for biomolecule–target substance binding. Typically, biomolecules reach the target substances through random diffusion, and to overcome the limitations associated herewith, biosensors have been integrated with alternating current (AC) electrokinetics (ACEK) technology. ACEK, through the application of alternating voltages, converts electrical energy into fluid motion, inducing pumping, mixing, concentration, and separation of the fluid. Its low power consumption makes it highly promising as a point-of-care diagnostic device. In this paper, we review the advancements in three ACEK technologies: AC electrothermal flow, AC electro-osmosis, and AC di-electrophoresis, to discuss the development of rapid biosensor fabrication methods based on electrical potential applications.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"19 7-8","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-14DOI: 10.1007/s13206-023-00126-y
My-Van Tieu, Duc Trung Pham, Hien T. Ngoc Le, Thi Xoan Hoang, Sungbo Cho
{"title":"Rapid and Ultrasensitive Detection of Staphylococcus aureus Using a Gold-Interdigitated Single-Wave-Shaped Electrode (Au-ISWE) Electrochemical Biosensor","authors":"My-Van Tieu, Duc Trung Pham, Hien T. Ngoc Le, Thi Xoan Hoang, Sungbo Cho","doi":"10.1007/s13206-023-00126-y","DOIUrl":"https://doi.org/10.1007/s13206-023-00126-y","url":null,"abstract":"","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"35 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134953791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-17DOI: 10.1007/s13206-023-00125-z
Se Young Choi, Mirinae Kim, Su Jeong Kang, Young Wook Choi, Sejung Maeng, Sung-Hwan Kim, In Ho Chang
{"title":"Enhanced Antitumor Effect of the Combination of Bacille Calmette-Guérin and an Immune Checkpoint Inhibitor in Bladder Cancer-On-a-Chip","authors":"Se Young Choi, Mirinae Kim, Su Jeong Kang, Young Wook Choi, Sejung Maeng, Sung-Hwan Kim, In Ho Chang","doi":"10.1007/s13206-023-00125-z","DOIUrl":"https://doi.org/10.1007/s13206-023-00125-z","url":null,"abstract":"","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136032790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.1007/s13206-023-00122-2
Haeun Yu, Jaejeung Kim, Jianning Yu, Kyung-A Hyun, Jae-Yol Lim, Yeo-Jun Yoon, Sunyoung Park, Hyo-Il Jung
Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically affect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is difficult to isolate specific EVs that have target surface proteins that affect regenerative effects. To address these limitations, a new dual-mode horseshoe-shaped orifice micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specific SC-EVs on the beads using an eluent was developed. For effective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding efficiencies between the antibodies and specific SC-EVs for three repeated cycles using the dual-mode chip. CD63+ EVs collected by the chip exhibited higher cell viability and regeneration effects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects.
{"title":"Continuous Isolation of Stem-Cell-Derived Extracellular Vesicles (SC-EVs) by Recycled Magnetic Beads in Microfluidic Channels","authors":"Haeun Yu, Jaejeung Kim, Jianning Yu, Kyung-A Hyun, Jae-Yol Lim, Yeo-Jun Yoon, Sunyoung Park, Hyo-Il Jung","doi":"10.1007/s13206-023-00122-2","DOIUrl":"https://doi.org/10.1007/s13206-023-00122-2","url":null,"abstract":"Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically affect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is difficult to isolate specific EVs that have target surface proteins that affect regenerative effects. To address these limitations, a new dual-mode horseshoe-shaped orifice micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specific SC-EVs on the beads using an eluent was developed. For effective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding efficiencies between the antibodies and specific SC-EVs for three repeated cycles using the dual-mode chip. CD63+ EVs collected by the chip exhibited higher cell viability and regeneration effects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135592564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-21DOI: 10.1007/s13206-023-00119-x
Na Eun Lee, Ji Hye Hong, Seungmin Lee, Yong Kyoung Yoo, Kang Hyeon Kim, Jeong Soo Park, Cheonjung Kim, Junghyo Yoon, Dae Sung Yoon, Jeong Hoon Lee
{"title":"Integration of an Aptamer-Based Signal-On Probe and a Paper-Based Origami Preconcentrator for Small Molecule Biomarkers Detection","authors":"Na Eun Lee, Ji Hye Hong, Seungmin Lee, Yong Kyoung Yoo, Kang Hyeon Kim, Jeong Soo Park, Cheonjung Kim, Junghyo Yoon, Dae Sung Yoon, Jeong Hoon Lee","doi":"10.1007/s13206-023-00119-x","DOIUrl":"https://doi.org/10.1007/s13206-023-00119-x","url":null,"abstract":"","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136154594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.1007/s13206-023-00120-4
Seongsoo Kim, Jeeyeon Park, Hye-Won Kim, Jinsik Kim, Joonwon Bae, Min Hee Lee, Dong-Sik Shin
{"title":"Low-Loaded Polyethylene Glycol (PEG) Resin for High-Purity Peptide Synthesis and Cell Binding Assays","authors":"Seongsoo Kim, Jeeyeon Park, Hye-Won Kim, Jinsik Kim, Joonwon Bae, Min Hee Lee, Dong-Sik Shin","doi":"10.1007/s13206-023-00120-4","DOIUrl":"https://doi.org/10.1007/s13206-023-00120-4","url":null,"abstract":"","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135061140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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