Pub Date : 2007-05-01DOI: 10.1007/S12030-008-9006-0
K. Ishihara, B. Ando, M. Takai
{"title":"Phosphorylcholine Group-immobilized Surface Prepared on Polydimethylsiloxane Membrane by In Situ Reaction for Its Reduced Biofouling","authors":"K. Ishihara, B. Ando, M. Takai","doi":"10.1007/S12030-008-9006-0","DOIUrl":"https://doi.org/10.1007/S12030-008-9006-0","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 1","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2007-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/S12030-008-9006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52746350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-05-01DOI: 10.1007/s12030-008-9003-3
Jie Lu, Monty Liong, Sean Sherman, Tian Xia, Michael Kovochich, Andre E Nel, Jeffrey I Zink, Fuyuhiko Tamanoi
Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37 degrees C than at 4 degrees C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.
{"title":"Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells.","authors":"Jie Lu, Monty Liong, Sean Sherman, Tian Xia, Michael Kovochich, Andre E Nel, Jeffrey I Zink, Fuyuhiko Tamanoi","doi":"10.1007/s12030-008-9003-3","DOIUrl":"https://doi.org/10.1007/s12030-008-9003-3","url":null,"abstract":"<p><p>Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37 degrees C than at 4 degrees C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.</p>","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 2","pages":"89-95"},"PeriodicalIF":0.0,"publicationDate":"2007-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12030-008-9003-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-05-01DOI: 10.1007/S12030-008-9008-Y
Hyo-Jick Choi, D. Wendell, C. Montemagno
{"title":"Advances in Nano Biotic/Abiotic Hybrid Systems: Protein-Based Engineered Devices","authors":"Hyo-Jick Choi, D. Wendell, C. Montemagno","doi":"10.1007/S12030-008-9008-Y","DOIUrl":"https://doi.org/10.1007/S12030-008-9008-Y","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 1","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2007-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/S12030-008-9008-Y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52746443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-26DOI: 10.1007/S12030-007-0002-6
A. Cans, S. Dean, F. Reyes, C. Keating
{"title":"Synthesis and characterization of enzyme-Au bioconjugates: HRP and fluorescein-labeled HRP","authors":"A. Cans, S. Dean, F. Reyes, C. Keating","doi":"10.1007/S12030-007-0002-6","DOIUrl":"https://doi.org/10.1007/S12030-007-0002-6","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 1","pages":"12-22"},"PeriodicalIF":0.0,"publicationDate":"2007-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/S12030-007-0002-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52745678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-26DOI: 10.1007/S12030-007-0005-3
Gang Han, P. Ghosh, Mrinmoy De, V. Rotello
{"title":"Drug and gene delivery using gold nanoparticles","authors":"Gang Han, P. Ghosh, Mrinmoy De, V. Rotello","doi":"10.1007/S12030-007-0005-3","DOIUrl":"https://doi.org/10.1007/S12030-007-0005-3","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 1","pages":"40-45"},"PeriodicalIF":0.0,"publicationDate":"2007-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/S12030-007-0005-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52745818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-01-01Epub Date: 2007-05-03DOI: 10.1007/s12030-007-0003-5
M Y Sha, S Penn, G Freeman, W E Doering
A dual-mode molecular beacon on a multiplexed substrate has been developed and applied to the measurement of unlabeled human viral RNA. The detection system is based on a combined surface-enhanced Raman scattering (SERS) and fluorescent molecular beacon assay that is assembled on Nanobarcodes™ particles. In this assay, a molecular beacon probe terminated with a fluorescent Raman label dye is conjugated to the metallic Nanobarcodes™ particles. The molecular beacon probe is a single-stranded oligonucleotide that has been designed with a hairpin structure that holds the dye at 3'-end close to the particle surface when the probe is attached through a 5'-thiol group. In this configuration, the SERS spectrum of the label was obtained and its fluorescence quenched because the dye is in very close proximity to a noble metal surface with nanoscale features (Nanobarcodes™ particles). The SERS signal decreased and the fluorescence signal increased when target viral RNA was captured by this molecular beacon probe. In addition, a hepatitis C virus reverse transcriptase-polymerase chain reaction (HCV RT-PCR) product was detected using this dual-mode beacon. The development of a multiplexed, label-free assay system with the reassurance offered by detection of two distinctly separate signals offers significant benefits for rapid molecular diagnostics.
{"title":"Detection of human viral RNA via a combined fluorescence and SERS molecular beacon assay.","authors":"M Y Sha, S Penn, G Freeman, W E Doering","doi":"10.1007/s12030-007-0003-5","DOIUrl":"10.1007/s12030-007-0003-5","url":null,"abstract":"<p><p>A dual-mode molecular beacon on a multiplexed substrate has been developed and applied to the measurement of unlabeled human viral RNA. The detection system is based on a combined surface-enhanced Raman scattering (SERS) and fluorescent molecular beacon assay that is assembled on Nanobarcodes™ particles. In this assay, a molecular beacon probe terminated with a fluorescent Raman label dye is conjugated to the metallic Nanobarcodes™ particles. The molecular beacon probe is a single-stranded oligonucleotide that has been designed with a hairpin structure that holds the dye at 3'-end close to the particle surface when the probe is attached through a 5'-thiol group. In this configuration, the SERS spectrum of the label was obtained and its fluorescence quenched because the dye is in very close proximity to a noble metal surface with nanoscale features (Nanobarcodes™ particles). The SERS signal decreased and the fluorescence signal increased when target viral RNA was captured by this molecular beacon probe. In addition, a hepatitis C virus reverse transcriptase-polymerase chain reaction (HCV RT-PCR) product was detected using this dual-mode beacon. The development of a multiplexed, label-free assay system with the reassurance offered by detection of two distinctly separate signals offers significant benefits for rapid molecular diagnostics.</p>","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"3 1","pages":"23-30"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37776487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimicrobial surfaces of viologen-quaternized poly((2-dimethyl amino)ethyl methacrylate)-Si(100) hybrids from surface-initiated atom transfer radical polymerization","authors":"F. Xu, S. Yuan, S. Pehkonen, E. Kang, K. Neoh","doi":"10.1007/BF02697267","DOIUrl":"https://doi.org/10.1007/BF02697267","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"2 1","pages":"123-134"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02697267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52513154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ai-Rong Liu, D. Qian, Meng Chen, T. Wakayama, C. Nakamura, N. Zorin, J. Miyake
{"title":"Electrochemical properties of hydrogenase on glass carbon electrodes modified with carbon nanotubes","authors":"Ai-Rong Liu, D. Qian, Meng Chen, T. Wakayama, C. Nakamura, N. Zorin, J. Miyake","doi":"10.1007/BF02697268","DOIUrl":"https://doi.org/10.1007/BF02697268","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"2 1","pages":"135-141"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02697268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52513184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Simultaneous detection of DNA-binding proteins using exo-Taq-based reaction","authors":"T. Fukumori, H. Miyachi, K. Yokoyama","doi":"10.1007/BF02697266","DOIUrl":"https://doi.org/10.1007/BF02697266","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"4 1","pages":"117-121"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02697266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52513061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three approaches to assembling nano-bio-machines using molecular motors","authors":"Y. Hiratsuka, T. Kamei, N. Yumoto, T. Uyeda","doi":"10.1007/BF02697265","DOIUrl":"https://doi.org/10.1007/BF02697265","url":null,"abstract":"","PeriodicalId":88286,"journal":{"name":"Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences","volume":"2 1","pages":"101-115"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02697265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52512991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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