Pub Date : 2013-01-01Epub Date: 2013-09-03DOI: 10.1155/2013/752620
Jessica M Gluck, Jennifer Chyu, Connor Delman, Sepideh Heydarkhan-Hagvall, W Robb MacLellan, Richard J Shemin
The relationship between stem cell niches in vivo and their surrounding microenvironment is still relatively unknown. Recent advances have indicated that extrinsic factors within the cardiovascular progenitor cell niche influence maintenance of a multipotent state as well as drive cell-fate decisions. We have previously shown the direct effects of extracellular matrix (ECM) proteins and have now investigated the effects of dimension on the induction of a cardiovascular progenitor cell (CPC) population. We have shown here that the three-dimensionality of a hyaluronan-based hydrogel greatly induces a CPC population, as marked by Flk-1. We have compared the effects of a 3D microenvironment to those of conventional 2D cell culture practices and have found that the 3D microenvironment potently induces a progenitor cell state.
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The mechanical bidomain model is a new mathematical description of the elastic behavior of cardiac tissue. Its primary advantage over previous models is that it accounts for forces acting across the cell membrane arising form differences in the displacement of the intracellular and extracellular spaces. In this review, I describe the development of the mechanical bidomain model. I emphasize new predictions of the model, such as the existence of boundary layers at the tissue surface where the membrane forces are large, and pressure differences between the intracellular and extracellular spaces. Although the theoretical analysis is quite mathematical, I highlight the types of experiments that could be used to test the model predictions. Finally, I present open questions about the mechanical bidomain model that may be productive future directions for research.
{"title":"The Mechanical Bidomain Model: A Review.","authors":"Bradley J Roth","doi":"10.1155/2013/863689","DOIUrl":"https://doi.org/10.1155/2013/863689","url":null,"abstract":"<p><p>The mechanical bidomain model is a new mathematical description of the elastic behavior of cardiac tissue. Its primary advantage over previous models is that it accounts for forces acting across the cell membrane arising form differences in the displacement of the intracellular and extracellular spaces. In this review, I describe the development of the mechanical bidomain model. I emphasize new predictions of the model, such as the existence of boundary layers at the tissue surface where the membrane forces are large, and pressure differences between the intracellular and extracellular spaces. Although the theoretical analysis is quite mathematical, I highlight the types of experiments that could be used to test the model predictions. Finally, I present open questions about the mechanical bidomain model that may be productive future directions for research.</p>","PeriodicalId":89790,"journal":{"name":"ISRN tissue engineering","volume":"2013 ","pages":"863689"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/863689","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31563108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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