首页 > 最新文献

BMC Dermatology最新文献

英文 中文
A seven-year retrospective analysis of patch test data in a cohort of patients with contact dermatitis in Sri Lanka. 对斯里兰卡接触性皮炎患者队列中斑贴试验数据的7年回顾性分析
Q2 Medicine Pub Date : 2019-07-10 DOI: 10.1186/s12895-019-0090-8
B S D P Keragala, H M M T B Herath, T S Keragala, M A M H Malavi, Chaturaka Rodrigo, C N Gunasekera

Background: Patch testing with a baseline series is a common tool employed when the sensitizing agent in contact dermatitis is unclear. However, for Asian countries, there are no locally validated baseline series to utilize in screening.

Methods: We completed a retrospective analysis of all patients that had undergone patch testing with the European Baseline series, Shoe Series or Comprehensive International Baseline series, over 7 years from 2012 to 2018 in a tertiary care reference dermatology clinic in Sri Lanka to evaluate the suitability of these investigations to identify causes for contact dermatitis in the local study population.

Results: Out of 438 patients tested, 239 (54.8%) reacted to at least one substance in the series. The Shoe Series was significantly more likely to yield a positive result than the European Baseline Series (70.2% vs 46.9%, p < 0.05). The top three sensitizers identified by all series were nickel sulfate (16%, 70/438), p-phenylenediamine (12.3%, 54/438) and 2-mercaptobenzothiazole or mercapto mix (10.5%, 46/438).

Conclusion: Shoe series has a comparatively high yield in the local population compared to European Baseline series. Since little less than half of the study population did not have any reactivity to any of the allergens tested it is important to develop or modify and validate a locally relevant, more suitable baseline series which is based on the Shoe Series in Sri Lanka. This is further evidence for the continuously changing nature of allergens in the environment and the need to modify existing patch testing standards accordingly.

背景:当接触性皮炎的致敏剂不清楚时,基线系列的斑贴试验是常用的工具。然而,对于亚洲国家,没有当地有效的基线系列用于筛查。方法:我们对斯里兰卡一家三级参考皮肤科诊所2012年至2018年7年间接受欧洲基线系列、鞋类系列或综合国际基线系列补丁试验的所有患者进行了回顾性分析,以评估这些调查在确定当地研究人群接触性皮炎病因方面的适用性。结果:在测试的438例患者中,239例(54.8%)对该系列中的至少一种物质有反应。鞋系列比欧洲基线系列更有可能产生阳性结果(70.2% vs 46.9%), p结论:与欧洲基线系列相比,鞋系列在当地人群中具有相对较高的产出率。由于不到一半的研究人群对测试的任何过敏原没有任何反应性,因此重要的是开发或修改和验证一个与当地相关的、更合适的基线系列,该系列基于斯里兰卡的鞋子系列。这进一步证明了环境中过敏原的性质在不断变化,需要相应地修改现有的贴片测试标准。
{"title":"A seven-year retrospective analysis of patch test data in a cohort of patients with contact dermatitis in Sri Lanka.","authors":"B S D P Keragala,&nbsp;H M M T B Herath,&nbsp;T S Keragala,&nbsp;M A M H Malavi,&nbsp;Chaturaka Rodrigo,&nbsp;C N Gunasekera","doi":"10.1186/s12895-019-0090-8","DOIUrl":"https://doi.org/10.1186/s12895-019-0090-8","url":null,"abstract":"<p><strong>Background: </strong>Patch testing with a baseline series is a common tool employed when the sensitizing agent in contact dermatitis is unclear. However, for Asian countries, there are no locally validated baseline series to utilize in screening.</p><p><strong>Methods: </strong>We completed a retrospective analysis of all patients that had undergone patch testing with the European Baseline series, Shoe Series or Comprehensive International Baseline series, over 7 years from 2012 to 2018 in a tertiary care reference dermatology clinic in Sri Lanka to evaluate the suitability of these investigations to identify causes for contact dermatitis in the local study population.</p><p><strong>Results: </strong>Out of 438 patients tested, 239 (54.8%) reacted to at least one substance in the series. The Shoe Series was significantly more likely to yield a positive result than the European Baseline Series (70.2% vs 46.9%, p < 0.05). The top three sensitizers identified by all series were nickel sulfate (16%, 70/438), p-phenylenediamine (12.3%, 54/438) and 2-mercaptobenzothiazole or mercapto mix (10.5%, 46/438).</p><p><strong>Conclusion: </strong>Shoe series has a comparatively high yield in the local population compared to European Baseline series. Since little less than half of the study population did not have any reactivity to any of the allergens tested it is important to develop or modify and validate a locally relevant, more suitable baseline series which is based on the Shoe Series in Sri Lanka. This is further evidence for the continuously changing nature of allergens in the environment and the need to modify existing patch testing standards accordingly.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0090-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37133831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Diaper dermatitis: a survey of risk factors in Thai children aged under 24 months. 尿布皮炎:泰国24个月以下儿童的危险因素调查。
Q2 Medicine Pub Date : 2019-07-02 DOI: 10.1186/s12895-019-0089-1
Chonnakarn Sukhneewat, Jitjira Chaiyarit, Leelawadee Techasatian

Background: To identify the prevalence and risk factors associated with diaper dermatitis in Thai children aged 1-24 months.

Methods: This was a cross-sectional study of 1153 participants using structural questionnaires, which was conducted at Khon Kaen University Faculty of Medicine Pediatric department in Thailand. Univariate and multivariate logistic regression analyses were used to test the association between diaper dermatitis and its possible risk factors.

Results: The prevalence of diaper dermatitis among the study population was 36.1%, a rate which significantly decreased with age. The highest prevalence was found in subjects who were one to six months old. Risk factors that had a statistically significant association with diaper dermatitis in both univariate and multivariate analysis were i) diaper changing fewer than three times/night, ii) previous episodes of diaper rash, iii) using cloth diapers, and iv) topical application of baby talcum powder to the diaper area.

Conclusions: Frequent diaper changings during the daytime do not compensate for fewer changings during the night. Moreover, the use of baby talcum powder on the diaper area significantly increased the risk of diaper dermatitis among the study population. These findings should be applied in future preventive strategies for diaper dermatitis in this age group.

背景:了解泰国1-24月龄儿童尿布皮炎的患病率及相关危险因素。方法:这是一项横断面研究,1153名参与者使用结构性问卷,在泰国孔敬大学医学院儿科进行。采用单因素和多因素logistic回归分析来检验尿布皮炎及其可能危险因素之间的关系。结果:研究人群尿布皮炎患病率为36.1%,随年龄增长明显下降。在一到六个月大的孩子中患病率最高。在单因素和多因素分析中,与尿布皮炎有统计学显著关联的危险因素是:1)每晚换尿布少于3次,2)既往尿布疹发作,3)使用布尿布,4)在尿布部位局部使用婴儿滑石粉。结论:白天频繁换尿布并不能弥补夜间换尿布次数的减少。此外,在研究人群中,在尿布部位使用婴儿爽身粉显著增加了尿布皮炎的风险。这些发现应应用于该年龄组尿布皮炎的未来预防策略。
{"title":"Diaper dermatitis: a survey of risk factors in Thai children aged under 24 months.","authors":"Chonnakarn Sukhneewat,&nbsp;Jitjira Chaiyarit,&nbsp;Leelawadee Techasatian","doi":"10.1186/s12895-019-0089-1","DOIUrl":"https://doi.org/10.1186/s12895-019-0089-1","url":null,"abstract":"<p><strong>Background: </strong>To identify the prevalence and risk factors associated with diaper dermatitis in Thai children aged 1-24 months.</p><p><strong>Methods: </strong>This was a cross-sectional study of 1153 participants using structural questionnaires, which was conducted at Khon Kaen University Faculty of Medicine Pediatric department in Thailand. Univariate and multivariate logistic regression analyses were used to test the association between diaper dermatitis and its possible risk factors.</p><p><strong>Results: </strong>The prevalence of diaper dermatitis among the study population was 36.1%, a rate which significantly decreased with age. The highest prevalence was found in subjects who were one to six months old. Risk factors that had a statistically significant association with diaper dermatitis in both univariate and multivariate analysis were i) diaper changing fewer than three times/night, ii) previous episodes of diaper rash, iii) using cloth diapers, and iv) topical application of baby talcum powder to the diaper area.</p><p><strong>Conclusions: </strong>Frequent diaper changings during the daytime do not compensate for fewer changings during the night. Moreover, the use of baby talcum powder on the diaper area significantly increased the risk of diaper dermatitis among the study population. These findings should be applied in future preventive strategies for diaper dermatitis in this age group.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0089-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37381520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Rationale, objectives and design of PURE, a prospective registry of patients with moderate to severe chronic plaque psoriasis in Canada and Latin America. PURE的基本原理、目的和设计,是一项加拿大和拉丁美洲中重度慢性斑块性银屑病患者的前瞻性登记研究。
Q2 Medicine Pub Date : 2019-06-21 DOI: 10.1186/s12895-019-0087-3
Kim A Papp, Melinda Gooderham, Jennifer Beecker, Charles W Lynde, Isabelle Delorme, Ignacio Dei-Cas, Lorne Albrecht, Emmanouil Rampakakis, John S Sampalis, Antonio Vieira, Shamiza Hussein, Olivier Chambenoit, Lenka Rihakova

Background: Treatment options for the management of moderate to severe plaque psoriasis include phototherapy, oral systemic agents, and biologic therapy. Secukinumab, a fully human monoclonal antibody that selectively targets IL-17A, is the first IL-17 antagonist approved for this patient population. Long-term observational data are required for establishing the true population-based benefit-risk ratio of approved treatments. PURE is a multinational registry that will assess the real-world safety and effectiveness of secukinumab and other approved therapies in the management of patients with moderate to severe psoriasis.

Methods: This is a multinational (Canadian and Latin American), prospective, observational study of adult patients with moderate to severe psoriasis that initiate treatment with secukinumab or other approved therapies as per local standard of care. A total of 2500 patients (1250 per cohort) will be recruited in the practices of hospital and community dermatologists. Decision regarding treatment must have been reached prior to and independent of patient enrollment in the study. The study includes a 5-year follow-up with recommended assessments at Baseline, 3 and 6 months post-Baseline, and every 6 months thereafter. The primary objective of the study is safety. Secondary outcome measures relate to effectiveness (Investigator's Global Assessment -IGA mod 2011-, Psoriasis Areas and Severity Index, Body Surface Area), patient reported outcomes (Dermatology Life Quality Index, Work Productivity and Activity Impairment Questionnaire, Hospital Anxiety and Depression Scale, Psoriasis Epidemiology Screening Tool, Psoriasis Symptom Diary, and Treatment Satisfaction Questionnaire), and healthcare resource utilization.

Discussion: This is the first observational study in Canada and Latin America assessing the real-world safety and effectiveness of secukinumab in the management of moderate to severe psoriasis. The extensive clinical, patient-reported and health economic outcomes collected will allow the comprehensive evaluation of this new treatment in comparison to other approved therapies.

Trial registration: ClinicalTrials.gov Identifier: NCT02786186 ; date of registration: May 30, 2016.

背景:中重度斑块型银屑病的治疗选择包括光疗、口服全身药物和生物治疗。Secukinumab是一种选择性靶向IL-17A的全人源单克隆抗体,是首个被批准用于该患者群体的IL-17拮抗剂。需要长期观察数据来确定批准治疗的真正基于人群的获益-风险比。PURE是一个跨国注册项目,旨在评估secukinumab和其他已批准疗法在治疗中重度牛皮癣患者中的安全性和有效性。方法:这是一项多国(加拿大和拉丁美洲)的前瞻性观察性研究,研究对象是中度至重度牛皮癣成年患者,这些患者开始接受secukinumab或其他根据当地护理标准批准的疗法治疗。将在医院和社区皮肤科医生的实践中招募总计2500名患者(每队列1250名)。关于治疗的决定必须在患者入组研究之前做出,并且独立于患者入组研究。该研究包括5年随访,在基线、基线后3个月和6个月以及之后每6个月进行推荐评估。这项研究的主要目的是安全性。次要结果测量与有效性(研究者全球评估- iga模型2011-,牛皮癣面积和严重程度指数,体表面积),患者报告的结果(皮肤病生活质量指数,工作效率和活动障碍问卷,医院焦虑和抑郁量表,牛皮癣流行病学筛查工具,牛皮癣症状日记和治疗满意度问卷)以及医疗保健资源利用有关。讨论:这是加拿大和拉丁美洲的第一个观察性研究,评估了secukinumab治疗中重度牛皮癣的安全性和有效性。广泛的临床、患者报告和收集的健康经济结果将允许对这种新疗法与其他批准的疗法进行综合评估。试验注册:ClinicalTrials.gov标识符:NCT02786186;注册日期:2016年5月30日。
{"title":"Rationale, objectives and design of PURE, a prospective registry of patients with moderate to severe chronic plaque psoriasis in Canada and Latin America.","authors":"Kim A Papp,&nbsp;Melinda Gooderham,&nbsp;Jennifer Beecker,&nbsp;Charles W Lynde,&nbsp;Isabelle Delorme,&nbsp;Ignacio Dei-Cas,&nbsp;Lorne Albrecht,&nbsp;Emmanouil Rampakakis,&nbsp;John S Sampalis,&nbsp;Antonio Vieira,&nbsp;Shamiza Hussein,&nbsp;Olivier Chambenoit,&nbsp;Lenka Rihakova","doi":"10.1186/s12895-019-0087-3","DOIUrl":"https://doi.org/10.1186/s12895-019-0087-3","url":null,"abstract":"<p><strong>Background: </strong>Treatment options for the management of moderate to severe plaque psoriasis include phototherapy, oral systemic agents, and biologic therapy. Secukinumab, a fully human monoclonal antibody that selectively targets IL-17A, is the first IL-17 antagonist approved for this patient population. Long-term observational data are required for establishing the true population-based benefit-risk ratio of approved treatments. PURE is a multinational registry that will assess the real-world safety and effectiveness of secukinumab and other approved therapies in the management of patients with moderate to severe psoriasis.</p><p><strong>Methods: </strong>This is a multinational (Canadian and Latin American), prospective, observational study of adult patients with moderate to severe psoriasis that initiate treatment with secukinumab or other approved therapies as per local standard of care. A total of 2500 patients (1250 per cohort) will be recruited in the practices of hospital and community dermatologists. Decision regarding treatment must have been reached prior to and independent of patient enrollment in the study. The study includes a 5-year follow-up with recommended assessments at Baseline, 3 and 6 months post-Baseline, and every 6 months thereafter. The primary objective of the study is safety. Secondary outcome measures relate to effectiveness (Investigator's Global Assessment -IGA mod 2011-, Psoriasis Areas and Severity Index, Body Surface Area), patient reported outcomes (Dermatology Life Quality Index, Work Productivity and Activity Impairment Questionnaire, Hospital Anxiety and Depression Scale, Psoriasis Epidemiology Screening Tool, Psoriasis Symptom Diary, and Treatment Satisfaction Questionnaire), and healthcare resource utilization.</p><p><strong>Discussion: </strong>This is the first observational study in Canada and Latin America assessing the real-world safety and effectiveness of secukinumab in the management of moderate to severe psoriasis. The extensive clinical, patient-reported and health economic outcomes collected will allow the comprehensive evaluation of this new treatment in comparison to other approved therapies.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov Identifier: NCT02786186 ; date of registration: May 30, 2016.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2019-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0087-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37358071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
A multi-item Physician Global Assessment scale to assess psoriasis disease severity: validation based on four phase III tofacitinib studies. 评估牛皮癣疾病严重程度的多项目医师全球评估量表:基于四项III期托法替尼研究的验证
Q2 Medicine Pub Date : 2019-06-07 DOI: 10.1186/s12895-019-0088-2
Kristina Callis Duffin, Andrew G Bushmakin, Joseph C Cappelleri, Lotus Mallbris, Carla Mamolo

Background: Several different Physician Global Assessment (PGA) versions have been used in clinical studies as a co-primary end point to evaluate psoriasis severity. Tofacitinib is an oral Janus kinase inhibitor. We performed an analysis of the PGA using data from studies of tofacitinib in moderate to severe chronic plaque psoriasis.

Methods: Data from 3641 patients with moderate to severe chronic plaque psoriasis, enrolled in one of four phase III tofacitinib studies (OPT Pivotal 1 and 2, OPT Compare and OPT Retreatment), were used to evaluate a three-item PGA scale.

Results: Confirmatory Factor Analyses showed that equal weighting of the three items (erythema, induration and scaling) was appropriate. The PGA demonstrated acceptable test-retest reliability (Intraclass Correlation Coefficient, 0.7) and internal consistency (Cronbach's Coefficient Alpha ≥ 0.9 at primary time points). The Clinically Important Difference was estimated as 0.55 (95% confidence interval: 0.546-0.563). Known-group validity was shown by demonstrating that PGA scores could discriminate between different degrees of disease severity. The PGA was significantly correlated with other clinical end points in the studies (Psoriasis Area and Severity Index, r = 0.75-0.79; Dermatology Life Quality Index, r = 0.44-0.57; Patient Global Assessment, r = 0.66-0.72).

Conclusions: Consistent with previous findings from a phase II study, these results indicate that this PGA is a valid, reliable instrument for evaluating disease severity in clinical studies of psoriasis.

背景:几种不同的医师整体评估(PGA)版本已被用于临床研究,作为评估牛皮癣严重程度的共同主要终点。托法替尼是一种口服Janus激酶抑制剂。我们使用托法替尼治疗中重度慢性斑块型银屑病的研究数据对PGA进行了分析。方法:来自3641例中至重度慢性斑块型银屑病患者的数据,这些患者参加了四项托法替尼III期研究(OPT Pivotal 1和2,OPT Compare和OPT Retreatment)中的一项,用于评估三项PGA量表。结果:验证性因子分析显示,红斑、硬结和结垢三项指标的权重相等。PGA表现出可接受的重测信度(类内相关系数为0.7)和内部一致性(主要时间点的Cronbach系数α≥0.9)。临床重要差异估计为0.55(95%可信区间:0.546-0.563)。已知组效度证明PGA评分可以区分不同程度的疾病严重程度。PGA与研究中的其他临床终点显著相关(银屑病面积和严重程度指数,r = 0.75-0.79;皮肤科生活质量指数,r = 0.44-0.57;患者总体评估,r = 0.66-0.72)。结论:与先前的II期研究结果一致,这些结果表明PGA是评估银屑病临床研究中疾病严重程度的有效、可靠的工具。
{"title":"A multi-item Physician Global Assessment scale to assess psoriasis disease severity: validation based on four phase III tofacitinib studies.","authors":"Kristina Callis Duffin,&nbsp;Andrew G Bushmakin,&nbsp;Joseph C Cappelleri,&nbsp;Lotus Mallbris,&nbsp;Carla Mamolo","doi":"10.1186/s12895-019-0088-2","DOIUrl":"https://doi.org/10.1186/s12895-019-0088-2","url":null,"abstract":"<p><strong>Background: </strong>Several different Physician Global Assessment (PGA) versions have been used in clinical studies as a co-primary end point to evaluate psoriasis severity. Tofacitinib is an oral Janus kinase inhibitor. We performed an analysis of the PGA using data from studies of tofacitinib in moderate to severe chronic plaque psoriasis.</p><p><strong>Methods: </strong>Data from 3641 patients with moderate to severe chronic plaque psoriasis, enrolled in one of four phase III tofacitinib studies (OPT Pivotal 1 and 2, OPT Compare and OPT Retreatment), were used to evaluate a three-item PGA scale.</p><p><strong>Results: </strong>Confirmatory Factor Analyses showed that equal weighting of the three items (erythema, induration and scaling) was appropriate. The PGA demonstrated acceptable test-retest reliability (Intraclass Correlation Coefficient, 0.7) and internal consistency (Cronbach's Coefficient Alpha ≥ 0.9 at primary time points). The Clinically Important Difference was estimated as 0.55 (95% confidence interval: 0.546-0.563). Known-group validity was shown by demonstrating that PGA scores could discriminate between different degrees of disease severity. The PGA was significantly correlated with other clinical end points in the studies (Psoriasis Area and Severity Index, r = 0.75-0.79; Dermatology Life Quality Index, r = 0.44-0.57; Patient Global Assessment, r = 0.66-0.72).</p><p><strong>Conclusions: </strong>Consistent with previous findings from a phase II study, these results indicate that this PGA is a valid, reliable instrument for evaluating disease severity in clinical studies of psoriasis.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2019-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0088-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37034906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Sofosbuvir induced leucocytoclasic vasculitis: a case report. 索非布韦致白细胞破裂性血管炎1例。
Q2 Medicine Pub Date : 2019-05-17 DOI: 10.1186/s12895-019-0086-4
Elena Campione, Annunziata Dattola, Monia Di Prete, Daniele Di Paolo, Amedeo Ferlosio, Augusto Orlandi, Luca Bianchi

Background: We describe a case of leucocytoclasic vasculitis induced by Sofosbuvir and its disappearence after the end of the therapy. The hepatitis C virus, firstly described in 1989, is a major global health problem, with high morbidity and mortality. We observed a temporal relationship between the treatment and the onset of vasculitis. We emphasize the multidisciplinary approach to the patients with liver disease to improve the quality of life of these patients.

Case presentation: A 53-year-old Caucasian man with a history of hepatitis C virus genotype 1 infection was examined at our Department of Dermatology for the occurrence of palpable purpura. The patient referred that the first appearance of the dermatoses was about one month after initiation of therapy with Sofosbuvir for hepatitis C.

Conclusions: Vasculitis appeared after the beginning of Sofosbuvir and, even though it was treated with different medications proved to be effective, it disappeared only after the conclusion of the therapy, giving a strong evidence to be a drug eruption.

背景:我们描述了一例由索非布韦引起的白细胞破坏性血管炎,并在治疗结束后消失。丙型肝炎病毒于1989年首次被描述,是一个主要的全球卫生问题,发病率和死亡率都很高。我们观察到治疗与血管炎发病之间的时间关系。我们强调肝病患者的多学科方法,以提高这些患者的生活质量。病例介绍:一名53岁的白人男性,有丙型肝炎病毒基因型感染史,在我皮肤科检查可触及的紫癜的发生。患者提到,该皮肤病首次出现是在索非布韦治疗丙型肝炎开始一个月后。结论:索非布韦开始治疗后出现血管炎,尽管用不同的药物治疗均有效,但在治疗结束后才消失,有力地证明是药疹。
{"title":"Sofosbuvir induced leucocytoclasic vasculitis: a case report.","authors":"Elena Campione,&nbsp;Annunziata Dattola,&nbsp;Monia Di Prete,&nbsp;Daniele Di Paolo,&nbsp;Amedeo Ferlosio,&nbsp;Augusto Orlandi,&nbsp;Luca Bianchi","doi":"10.1186/s12895-019-0086-4","DOIUrl":"https://doi.org/10.1186/s12895-019-0086-4","url":null,"abstract":"<p><strong>Background: </strong>We describe a case of leucocytoclasic vasculitis induced by Sofosbuvir and its disappearence after the end of the therapy. The hepatitis C virus, firstly described in 1989, is a major global health problem, with high morbidity and mortality. We observed a temporal relationship between the treatment and the onset of vasculitis. We emphasize the multidisciplinary approach to the patients with liver disease to improve the quality of life of these patients.</p><p><strong>Case presentation: </strong>A 53-year-old Caucasian man with a history of hepatitis C virus genotype 1 infection was examined at our Department of Dermatology for the occurrence of palpable purpura. The patient referred that the first appearance of the dermatoses was about one month after initiation of therapy with Sofosbuvir for hepatitis C.</p><p><strong>Conclusions: </strong>Vasculitis appeared after the beginning of Sofosbuvir and, even though it was treated with different medications proved to be effective, it disappeared only after the conclusion of the therapy, giving a strong evidence to be a drug eruption.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2019-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0086-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37247820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Pattern of skin diseases in children attending a dermatology clinic in a referral hospital in Wolaita Sodo, southern Ethiopia. 埃塞俄比亚南部Wolaita Sodo一家转诊医院皮肤科诊所就诊儿童的皮肤病模式。
Q2 Medicine Pub Date : 2019-04-08 DOI: 10.1186/s12895-019-0085-5
Abraham Getachew Kelbore, Philip Owiti, Anthony J Reid, Efa Ambaw Bogino, Lantesil Wondewosen, Blen Kassahun Dessu

Background: Epidemiological studies to determine the pattern of skin diseases among children are important for proper health care planning and management. The purpose of this study was to describe the pattern of skin diseases among pediatric patients seen at a dermatology outpatient clinic of Wolaita Sodo Teaching and Referral Hospital, southern Ethiopia.

Method: We conducted a retrospective hospital-based, cross-sectional study between January 2016 and December 2017 at a teaching and referral hospital dermatology outpatient department. All children younger than 15 years presenting with newly-diagnosed skin diseases were included. Diagnosis was mainly made clinically, with some laboratory support.

Results: A total of 1704 children with 1869 new skin diagnoses were included, of whom 139 (8.2%) had more than one disease. Of the children, 52.4% were males and 44.9% within the age-group 5-10 years. Eczematous dermatitis accounted for the largest group (23.9%, n = 447) of skin conditions followed by bacterial infections (21.3%, n = 398), fungal infections (18.8%, n = 351) and infestations (9.9%, n = 185). Seasonal variation was demonstrated, with eczematous conditions and bacterial infections being higher during autumn and winter.

Conclusion: Overall, eczema, bacterial and fungal infections were the three major pediatric skin diseases occurring among children attending this hospital's outpatient department. There was seasonal variation in some of the skin diseases. This study gives a snapshot of skin disorders presenting to hospital in children in southern Ethiopia and may help to plan dermatology service expansion, educational programs and preventive measures.

背景:确定儿童皮肤病模式的流行病学研究对于正确的医疗保健规划和管理很重要。本研究的目的是描述在埃塞俄比亚南部Wolaita Sodo教学和转诊医院皮肤科门诊就诊的儿科患者的皮肤病模式。所有15岁以下的儿童 包括新诊断的皮肤病患者的年数。诊断主要是在临床上进行的,并有一些实验室支持。结果:共纳入1704名儿童,1869例新的皮肤诊断,其中139例(8.2%)患有一种以上疾病。在这些儿童中,52.4%是男性,44.9%在5-10岁年龄组 年。湿疹性皮炎占最大组(23.9% = 447)的皮肤状况,随后是细菌感染(21.3% = 398),真菌感染(18.8%,n = 351)和侵扰(9.9% = 185)。季节变化已得到证实,湿疹性疾病和细菌感染在秋冬季节更高。结论:湿疹、细菌和真菌感染是该院门诊儿童常见的三大皮肤病。一些皮肤病有季节性变化。这项研究简要介绍了埃塞俄比亚南部儿童出现在医院的皮肤病,可能有助于规划皮肤科服务的扩展、教育计划和预防措施。
{"title":"Pattern of skin diseases in children attending a dermatology clinic in a referral hospital in Wolaita Sodo, southern Ethiopia.","authors":"Abraham Getachew Kelbore,&nbsp;Philip Owiti,&nbsp;Anthony J Reid,&nbsp;Efa Ambaw Bogino,&nbsp;Lantesil Wondewosen,&nbsp;Blen Kassahun Dessu","doi":"10.1186/s12895-019-0085-5","DOIUrl":"10.1186/s12895-019-0085-5","url":null,"abstract":"<p><strong>Background: </strong>Epidemiological studies to determine the pattern of skin diseases among children are important for proper health care planning and management. The purpose of this study was to describe the pattern of skin diseases among pediatric patients seen at a dermatology outpatient clinic of Wolaita Sodo Teaching and Referral Hospital, southern Ethiopia.</p><p><strong>Method: </strong>We conducted a retrospective hospital-based, cross-sectional study between January 2016 and December 2017 at a teaching and referral hospital dermatology outpatient department. All children younger than 15 years presenting with newly-diagnosed skin diseases were included. Diagnosis was mainly made clinically, with some laboratory support.</p><p><strong>Results: </strong>A total of 1704 children with 1869 new skin diagnoses were included, of whom 139 (8.2%) had more than one disease. Of the children, 52.4% were males and 44.9% within the age-group 5-10 years. Eczematous dermatitis accounted for the largest group (23.9%, n = 447) of skin conditions followed by bacterial infections (21.3%, n = 398), fungal infections (18.8%, n = 351) and infestations (9.9%, n = 185). Seasonal variation was demonstrated, with eczematous conditions and bacterial infections being higher during autumn and winter.</p><p><strong>Conclusion: </strong>Overall, eczema, bacterial and fungal infections were the three major pediatric skin diseases occurring among children attending this hospital's outpatient department. There was seasonal variation in some of the skin diseases. This study gives a snapshot of skin disorders presenting to hospital in children in southern Ethiopia and may help to plan dermatology service expansion, educational programs and preventive measures.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2019-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0085-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37131612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
A novel elastin gene frameshift mutation in a Russian family with cutis laxa: a case report. 在一个俄罗斯皮肤松弛症家庭中发现一种新的弹性蛋白基因移码突变:一个病例报告。
Q2 Medicine Pub Date : 2019-01-31 DOI: 10.1186/s12895-019-0084-6
E G Okuneva, A A Kozina, N V Baryshnikova, A Yu Krasnenko, K Yu Tsukanov, O I Klimchuk, E I Surkova, V V Ilinsky

Background: Cutis laxa (CL) is a rare connective tissue disorder characterized by loose, redundant, inelastic and wrinkled skin. Patients develop a prematurely aged appearance. Inheritance can be autosomal dominant or autosomal recessive. The X-linked form is now classified in the group of copper transport diseases. Autosomal dominant CL is characterized by wrinkled, redundant and sagging, inelastic skin and in some cases is associated with internal organ involvement.

Case presentation: We report a familial case of autosomal dominant CL, which includes a 33-year-old woman and her 11-year-old son with dry, thin and wrinkled skin that appeared prematurely aged. No serious involvement of internal organs was found. In both patients, we identified novel heterozygous mutation c.2323delG (p.Ala775fs) in exon 34 of elastin transcript NM_001278939.1. Similar frameshift mutations in the last exons of elastin gene were previously reported in patients with autosomal dominant CL.

Conclusions: Our results show a novel frameshift mutation that was found in patients with cutis laxa. Exome sequencing is effective and useful technology for properly diagnosis of diseases with similar phenotype to ensure proper treatment is provided.

背景:皮肤松弛症(Cutis laxa, CL)是一种罕见的结缔组织疾病,其特征是皮肤松弛、多余、无弹性和起皱。患者会出现过早衰老的外观。遗传可为常染色体显性遗传或常染色体隐性遗传。x -连锁形式现在被归类为铜转运疾病组。常染色体显性CL的特征是皮肤起皱、多余、下垂、无弹性,在某些情况下与内脏受累有关。病例介绍:我们报告一个家族性病例常染色体显性CL,其中包括一个33岁的妇女和她11岁的儿子干燥,薄和皱纹的皮肤,出现过早老化。没有发现严重的内脏受累。在这两例患者中,我们在弹性蛋白转录物NM_001278939.1的第34外显子中发现了新的杂合突变c.2323delG (p.Ala775fs)。类似的移码突变在弹性蛋白基因的最后外显子以前报道的患者常染色体显性CL。结论:我们的研究结果显示在皮肤松弛症患者中发现了一种新的移码突变。外显子组测序是正确诊断具有相似表型的疾病以确保提供适当治疗的有效和有用的技术。
{"title":"A novel elastin gene frameshift mutation in a Russian family with cutis laxa: a case report.","authors":"E G Okuneva,&nbsp;A A Kozina,&nbsp;N V Baryshnikova,&nbsp;A Yu Krasnenko,&nbsp;K Yu Tsukanov,&nbsp;O I Klimchuk,&nbsp;E I Surkova,&nbsp;V V Ilinsky","doi":"10.1186/s12895-019-0084-6","DOIUrl":"https://doi.org/10.1186/s12895-019-0084-6","url":null,"abstract":"<p><strong>Background: </strong>Cutis laxa (CL) is a rare connective tissue disorder characterized by loose, redundant, inelastic and wrinkled skin. Patients develop a prematurely aged appearance. Inheritance can be autosomal dominant or autosomal recessive. The X-linked form is now classified in the group of copper transport diseases. Autosomal dominant CL is characterized by wrinkled, redundant and sagging, inelastic skin and in some cases is associated with internal organ involvement.</p><p><strong>Case presentation: </strong>We report a familial case of autosomal dominant CL, which includes a 33-year-old woman and her 11-year-old son with dry, thin and wrinkled skin that appeared prematurely aged. No serious involvement of internal organs was found. In both patients, we identified novel heterozygous mutation c.2323delG (p.Ala775fs) in exon 34 of elastin transcript NM_001278939.1. Similar frameshift mutations in the last exons of elastin gene were previously reported in patients with autosomal dominant CL.</p><p><strong>Conclusions: </strong>Our results show a novel frameshift mutation that was found in patients with cutis laxa. Exome sequencing is effective and useful technology for properly diagnosis of diseases with similar phenotype to ensure proper treatment is provided.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0084-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36917803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Mucous membrane pemphigoid-associated paronychia with onychomadesis. 粘膜类天疱疮相关性甲沟炎伴甲发育。
Q2 Medicine Pub Date : 2019-01-23 DOI: 10.1186/s12895-019-0083-7
Salim Alkeraye, Sarah F Alsukait

Background: Mucous membrane pemphigoid (MMP) is an autoimmune blistering disease that is notoriously difficult to treat. Nail involvement in MMP is rare.

Case presentation: We report on a 58 years old man with severe MMP who presented with onychomadesis.

Conclusion: To our knowledge, mucous membrane pemphigoid associated paronychia and onychomadesis have not been reported before. We believe it is important for dermatologists to be aware of this entity.

背景:粘膜类天疱疮(MMP)是一种自身免疫性起泡性疾病,众所周知难以治疗。MMP患者很少累及指甲。病例介绍:我们报告了一个58岁的男性严重MMP谁提出了指骨发育。结论:据我们所知,粘膜类天疱疮合并甲沟炎和甲发育尚未见报道。我们认为这是重要的皮肤科医生意识到这个实体。
{"title":"Mucous membrane pemphigoid-associated paronychia with onychomadesis.","authors":"Salim Alkeraye,&nbsp;Sarah F Alsukait","doi":"10.1186/s12895-019-0083-7","DOIUrl":"https://doi.org/10.1186/s12895-019-0083-7","url":null,"abstract":"<p><strong>Background: </strong>Mucous membrane pemphigoid (MMP) is an autoimmune blistering disease that is notoriously difficult to treat. Nail involvement in MMP is rare.</p><p><strong>Case presentation: </strong>We report on a 58 years old man with severe MMP who presented with onychomadesis.</p><p><strong>Conclusion: </strong>To our knowledge, mucous membrane pemphigoid associated paronychia and onychomadesis have not been reported before. We believe it is important for dermatologists to be aware of this entity.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0083-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36889829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Proderm technology: a water- based lipid delivery system for dermatitis that penetrates viable epidermis and has antibacterial effects. Proderm技术:一种针对皮炎的水基脂质输送系统,可穿透活的表皮并具有抗菌作用。
Q2 Medicine Pub Date : 2019-01-22 DOI: 10.1186/s12895-019-0082-8
Alexandra Charruyer, Mats Silvander, Melinda Caputo-Janhager, Isabelle Raymond, Ruby Ghadially

Background: A defective skin barrier and bacterial colonization are two important factors in maintenance and progression of atopic dermatitis and chronic allergic/irritant hand dermatitis. A water-based lipid delivery system containing physiologic lipids was previously shown to be a useful adjunct in the treatment of hand dermatitis. We tested the ability of this formulation to penetrate into the viable epidermis and in addition assessed its antibacterial properties.

Methods: Epidermal penetration of the product was assessed by fluorescence microscopy. Recovery of Escherichia coli and Staphylococcus aureus MRSA from skin treated with Neosalus® foam was quantified.

Results: Components of Neosalus® penetrated the stratum corneum and were distributed throughout the viable epidermis. Neosalus® significantly decreased recovery of both Staphylococcus aureus and Escherichia coli from the skin surface.

Conclusions: The ability of components of Neosalus® to be taken up into the viable epidermis and potentially made available for incorporation into the barrier lipids, combined with antibacterial properties, indicate that this formulation may be valuable not only in chronic hand dermatitis, but also in various other forms of dermatitis.

Trial registration: Current Controlled Trials ISRCTN18191379 , 28/12/2018, retrospectively registered.

背景:皮肤屏障缺陷和细菌定植是特应性皮炎和慢性过敏性/刺激性手皮炎维持和发展的两个重要因素。含有生理性脂质的水基脂质输送系统以前被证明是治疗手部皮炎的有用辅助手段。我们测试了这种配方渗透到活表皮的能力,此外还评估了它的抗菌性能。方法:采用荧光显微镜观察产品表皮透性。定量分析Neosalus®泡沫处理皮肤中大肠杆菌和金黄色葡萄球菌MRSA的回收率。结果:Neosalus®成分渗透角质层,分布于活表皮。Neosalus®显著降低皮肤表面金黄色葡萄球菌和大肠杆菌的恢复。结论:Neosalus®的成分能够被活的表皮吸收,并可能被纳入屏障脂质,结合抗菌特性,表明该配方可能不仅对慢性手部皮炎有价值,而且对各种其他形式的皮炎也有价值。试验注册:当前对照试验ISRCTN18191379, 28/12/2018,回顾性注册。
{"title":"Proderm technology: a water- based lipid delivery system for dermatitis that penetrates viable epidermis and has antibacterial effects.","authors":"Alexandra Charruyer,&nbsp;Mats Silvander,&nbsp;Melinda Caputo-Janhager,&nbsp;Isabelle Raymond,&nbsp;Ruby Ghadially","doi":"10.1186/s12895-019-0082-8","DOIUrl":"https://doi.org/10.1186/s12895-019-0082-8","url":null,"abstract":"<p><strong>Background: </strong>A defective skin barrier and bacterial colonization are two important factors in maintenance and progression of atopic dermatitis and chronic allergic/irritant hand dermatitis. A water-based lipid delivery system containing physiologic lipids was previously shown to be a useful adjunct in the treatment of hand dermatitis. We tested the ability of this formulation to penetrate into the viable epidermis and in addition assessed its antibacterial properties.</p><p><strong>Methods: </strong>Epidermal penetration of the product was assessed by fluorescence microscopy. Recovery of Escherichia coli and Staphylococcus aureus MRSA from skin treated with Neosalus® foam was quantified.</p><p><strong>Results: </strong>Components of Neosalus® penetrated the stratum corneum and were distributed throughout the viable epidermis. Neosalus® significantly decreased recovery of both Staphylococcus aureus and Escherichia coli from the skin surface.</p><p><strong>Conclusions: </strong>The ability of components of Neosalus® to be taken up into the viable epidermis and potentially made available for incorporation into the barrier lipids, combined with antibacterial properties, indicate that this formulation may be valuable not only in chronic hand dermatitis, but also in various other forms of dermatitis.</p><p><strong>Trial registration: </strong>Current Controlled Trials ISRCTN18191379 , 28/12/2018, retrospectively registered.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2019-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-019-0082-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36886835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in microRNA expression between melanoma and healthy adjacent skin. 黑色素瘤与健康邻近皮肤microRNA表达差异
Q2 Medicine Pub Date : 2019-01-05 DOI: 10.1186/s12895-018-0081-1
Mariya Aksenenko, Nadezhda Palkina, Anna Komina, Liubov Tashireva, Tatiana Ruksha

Background: The tumor microenvironment is composed of cancer-associated fibroblasts, tumor-associated macrophages, endothelial cells, immune cells, signaling molecules and extracellular matrix structures, which closelycommunicate with the tumor via multiple mechanisms. MicroRNAs are paracrine regulators that provide a direct interaction between the microenvironment and cancer cells. In the presentstudy, we aimed to identify the microRNA expression profile in melanoma compared with thatin healthy adjacent skin, with a further assessment of altered microRNA signaling pathways and target genes.

Methods: Formalin-fixed paraffin-embedded (FFPE) melanoma tissue samples were separated by dissection into tumor and surrounding health tissue fragments. MicroRNA expression profiles were obtained by microarray using Gene Atlas Microarray System (Affymetrix, California, USA). To confirm microarray results real-time PCR was carried out. Bioinformatic analysis was performed using the DIANA-miRPath v.3.0 database. Target genes for miR-146a-5p were determined using three algorithms: TargetScan 7.0, miRWalk 2.0 and miRTarBase v.4.5.

Results: A microarray profiling revealed 143 microRNAs asdifferent in tumor versus adjacent tissues. Expression level of hsa-miR-146a-5p showedto be higher in melanoma cells as compared to thehealthy adjacent skin. The bioinformatic study has determined several signaling cascades associated with miR-146a-5p:Toll-like receptor pathway, NF-κB pathway, ErB pathway, and measles signaling pathway. The 38 target genes have been shown for miR-146a-5p of which NRAS gene is known asone of the most frequent mutated in melanoma.

Conclusions: Elucidation of the role of miR-146-a-5p in complex interactions between the tumor and the cells of healthy adjacent skin is necessary for our understanding of the mechanisms oftumor progression. Significant differences found between cancer cells and adjacent tissues in microRNA expression profile corresponding to divergent mRNA/protein levels in these structures should be taken into account when tumor samples characterization estimatedby high-throughput methods.

背景:肿瘤微环境由癌相关成纤维细胞、肿瘤相关巨噬细胞、内皮细胞、免疫细胞、信号分子和细胞外基质结构组成,它们通过多种机制与肿瘤密切沟通。microrna是旁分泌调节因子,在微环境和癌细胞之间提供直接的相互作用。在本研究中,我们旨在鉴定黑色素瘤与健康邻近皮肤中的microRNA表达谱,并进一步评估microRNA信号通路和靶基因的改变。方法:将福尔马林固定石蜡包埋(FFPE)黑色素瘤组织标本解剖分离为肿瘤和周围健康组织碎片。使用Gene Atlas microarray System (Affymetrix, California, USA)通过微阵列获得MicroRNA表达谱。为确认芯片结果,采用实时荧光定量PCR。采用DIANA-miRPath v.3.0数据库进行生物信息学分析。使用TargetScan 7.0、miRWalk 2.0和miRTarBase v.4.5三种算法确定miR-146a-5p的靶基因。结果:微阵列分析显示143个microrna在肿瘤和邻近组织中存在差异。与健康的邻近皮肤相比,hsa-miR-146a-5p在黑色素瘤细胞中的表达水平更高。生物信息学研究已经确定了几种与miR-146a-5p相关的信号级联:toll样受体通路、NF-κB通路、ErB通路和麻疹信号通路。miR-146a-5p的38个靶基因已经被发现,其中NRAS基因是黑色素瘤中最常见的突变之一。结论:阐明miR-146-a-5p在肿瘤与健康邻近皮肤细胞之间复杂相互作用中的作用对于我们理解肿瘤进展机制是必要的。当用高通量方法对肿瘤样品进行表征时,应考虑到癌细胞和邻近组织之间microRNA表达谱的显著差异,这些microRNA表达谱对应于这些结构中不同的mRNA/蛋白水平。
{"title":"Differences in microRNA expression between melanoma and healthy adjacent skin.","authors":"Mariya Aksenenko,&nbsp;Nadezhda Palkina,&nbsp;Anna Komina,&nbsp;Liubov Tashireva,&nbsp;Tatiana Ruksha","doi":"10.1186/s12895-018-0081-1","DOIUrl":"https://doi.org/10.1186/s12895-018-0081-1","url":null,"abstract":"<p><strong>Background: </strong>The tumor microenvironment is composed of cancer-associated fibroblasts, tumor-associated macrophages, endothelial cells, immune cells, signaling molecules and extracellular matrix structures, which closelycommunicate with the tumor via multiple mechanisms. MicroRNAs are paracrine regulators that provide a direct interaction between the microenvironment and cancer cells. In the presentstudy, we aimed to identify the microRNA expression profile in melanoma compared with thatin healthy adjacent skin, with a further assessment of altered microRNA signaling pathways and target genes.</p><p><strong>Methods: </strong>Formalin-fixed paraffin-embedded (FFPE) melanoma tissue samples were separated by dissection into tumor and surrounding health tissue fragments. MicroRNA expression profiles were obtained by microarray using Gene Atlas Microarray System (Affymetrix, California, USA). To confirm microarray results real-time PCR was carried out. Bioinformatic analysis was performed using the DIANA-miRPath v.3.0 database. Target genes for miR-146a-5p were determined using three algorithms: TargetScan 7.0, miRWalk 2.0 and miRTarBase v.4.5.</p><p><strong>Results: </strong>A microarray profiling revealed 143 microRNAs asdifferent in tumor versus adjacent tissues. Expression level of hsa-miR-146a-5p showedto be higher in melanoma cells as compared to thehealthy adjacent skin. The bioinformatic study has determined several signaling cascades associated with miR-146a-5p:Toll-like receptor pathway, NF-κB pathway, ErB pathway, and measles signaling pathway. The 38 target genes have been shown for miR-146a-5p of which NRAS gene is known asone of the most frequent mutated in melanoma.</p><p><strong>Conclusions: </strong>Elucidation of the role of miR-146-a-5p in complex interactions between the tumor and the cells of healthy adjacent skin is necessary for our understanding of the mechanisms oftumor progression. Significant differences found between cancer cells and adjacent tissues in microRNA expression profile corresponding to divergent mRNA/protein levels in these structures should be taken into account when tumor samples characterization estimatedby high-throughput methods.</p>","PeriodicalId":9014,"journal":{"name":"BMC Dermatology","volume":"19 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2019-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12895-018-0081-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36835635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
期刊
BMC Dermatology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1