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Development and Validation of a LC-MS/MS Method to DetermineLansoprazole in Human Plasma LC-MS/MS法测定人血浆中安索拉唑的建立与验证
Pub Date : 2015-04-27 DOI: 10.2174/1874065001508010007
R. Kachave, M. Kale, R. Wagh
A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for the determination of lansoprazole (LNZ) in human plasma. Pantaprazole was used as an internal standard (IS). Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Oasis HLB, Oasis Max, Varian Bond Elute Plexa, Orochem cartridges. The reconstituted samples were chromatographed on a thermo hypurity Advance, 50 X 4.6mm, 5 µ by using acetonitrile and 2 mM ammonium acetate solution(80:20 v/v) as the mobile phase at a flow rate of 1.0 mL/min. Detection was carried out LC-MS/MS (API 3000) in negative ion mode. The calibration curves obtained were linear (R 2 -0.999) over the concentration range of 4.50- 2800.00 ng/ml for lansaprazole. The results of the intra- and inter-day precision studies were well within the acceptable limits. The overall average recoveries of analyte and IS were found to be 92.10-99.11%. The analyte were found to be stable of stability study. Developed and validated analytical method was found to be simple, rapid, specific, sensitive, precise and cost effective than reported methods. The method has been successfully applied to the investigation of a preclinical pharmacokinetic study with desired precision and accuracy along with high throughput.
建立了一种快速、灵敏的液相色谱-串联质谱(LC-MS/MS)测定人血浆中兰索拉唑(lansoprazole, LNZ)的方法,并进行了验证。采用泮他拉唑作为内标。采用固相萃取技术,使用Oasis HLB、Oasis Max、Varian Bond Elute Plexa、Orochem萃取盒从人血浆中提取分析物和内标物。以乙腈和2 mM醋酸铵溶液(80:20 v/v)为流动相,流速为1.0 mL/min,在热纯Advance, 50 X 4.6mm, 5µ上进行色谱分析。采用LC-MS/MS (API 3000)在负离子模式下检测。在兰沙唑4.50 ~ 2800.00 ng/ml的浓度范围内,获得的校准曲线呈线性(r2 -0.999)。日间和日间精度研究的结果完全在可接受的范围内。分析物和IS的总体平均回收率为92.10 ~ 99.11%。稳定性研究表明,分析物是稳定的。开发和验证的分析方法与已有的方法相比,具有简单、快速、特异、敏感、精确和成本效益高的特点。该方法已成功应用于临床前药代动力学研究,具有较高的精密度和准确度。
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引用次数: 3
Simultaneous Determination of Paracetamol and Tramadol in PharmaceuticalTablets by Derivative UV-Vis Absorption Spectrophotometry 衍生紫外可见吸收分光光度法同时测定片剂中对乙酰氨基酚和曲马多的含量
Pub Date : 2015-03-31 DOI: 10.2174/1874065001508010001
Ahmad M. El-Zinati, M. Abdel-Latif
A comparative study of the use of first derivative zero order crossing spectra for the resolution of Paracetamol and Tramadol hydrochloride in mixtures has been achieved showing the success of the first derivative method in resolving and quantifying both compounds. Using the first derivative, rather than the second derivative, results in improved signal to noise ratio. The absorption spectra of prepared mixtures were scanned in the range of 200-500 nm. The linear concentration ranges were 25-112 and 6-48 µg mL -1 for paracetamol and Tramadol hydrochloride, respectively. The method has been successfully used for prediction of concentrations of both compounds in mixtures with good selectivity, high sensitivity and extremely low relative error. Statistical comparison was performed using t-test at 95% confidence level. There was no significant statistical difference between the results obtained by the first derivative method and the accepted values for both compounds. Also, the percentage errors were very low which adds to the merits of our work in terms of both sensitivity and accuracy.
对一阶导数零阶交叉光谱在对乙酰氨基酚和盐酸曲马多混合物中分离的比较研究表明,一阶导数方法在分离和定量这两种化合物方面取得了成功。使用一阶导数,而不是二阶导数,可以提高信噪比。对制备的混合物在200 ~ 500 nm范围内的吸收光谱进行扫描。对乙酰氨基酚和盐酸曲马多的线性浓度范围分别为25 ~ 112和6 ~ 48µg mL -1。该方法具有选择性好、灵敏度高、相对误差极低的优点,成功地用于预测混合物中两种化合物的浓度。统计学比较采用95%置信水平的t检验。用一阶导数法得到的结果与两种化合物的公认值之间没有显著的统计学差异。此外,错误率非常低,这增加了我们的工作在灵敏度和准确性方面的优点。
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引用次数: 14
Changes in the Porosity and Permeability of a Molecularly Imprinted Membrane Induced by the Adsorption of a Trace Quantity of Template 微量模板吸附诱导分子印迹膜孔隙率和渗透性的变化
Pub Date : 2013-10-14 DOI: 10.2174/1874065001307010022
Y. Yoshimi
It is known that the diffusive permeability of solutes within a thin layer of molecularly imprinted polymer (MIP) may be affected by specific binding of the MIP with its template molecule. This phenomenon, termed the gate effect, shows promise for the development of novel biomimetic sensors. However, the mechanism underlying this effect is still unclear; although the relationship between the specific adsorption of a template and the corresponding porosity and permeability of the polymeric film or membrane is very important, this association has not yet been examined in detail. We therefore studied this relationship using a molecularly imprinted self-supporting membrane (MISSM) possessing chiral specificity, specially developed as a tool for investigating the gate effect. Both the diffusive permeability and volume porosity of the MISSM were sensitive to the presence of the template compounds (D and L-phenylalanine) at concentrations as low as 5 µM, while, at the same time, insensitive to the enantiomer of the template. The relationship between the amount of adsorbed template and the equilibrium template concentration followed the expected Langmuir isotherm pattern, which indicates the thermodynamic homogeneity of binding sites in the MISSM. This study also demonstrated that the relative concentration of the adsorbed template in the membrane was only 3 ppm and relative site occupation was only 1% following exposure to a 5 µM concentration of the template. These results show that the gate effect may be advantageously exploited during application of MIPs in amplifiers or sensors offering high sensitivity. Molecularly imprinted polymers (MIPs) are synthetic polymers that contain specific binding sites formed by imprinting of a target molecule (or template) during the polymerization process. An MIP layer can be prepared by a simple and economical procedure (1-3). Before such MIPs may be employed as molecular recognition elements in chemical-sensing devices, however, it is necessary to develop a means of translating specific binding events at the MIP into an electric signal. The so-called gate effect, which refers to changes in the diffusive permeability of solutes within the MIP layer resulting from specific binding at the template, can be used as a mechanism for signal transference based on conductometry, utilizing changes in the ionic permeability of the MIP membrane associated with a specific interaction with the template (4-6). In this gate analogy, the template corresponds to the key while the MIP site which allows specific rebinding with the template corresponds to the keyhole. An amperometric method is also applicable, using a thin MIP layer grafted onto an electrode, in which the template can be detected by following changes in the faradic current resulting from the change in permeability of a redox marker across the MIP layer (7-11). The gate effect is a selective process capable of discriminating between
已知溶质在分子印迹聚合物(MIP)薄层内的扩散渗透性可能受到MIP与其模板分子特异性结合的影响。这种现象被称为门效应,为新型仿生传感器的发展提供了希望。然而,这种效应背后的机制尚不清楚;虽然模板的特异性吸附与相应的聚合物膜或膜的孔隙率和渗透率之间的关系非常重要,但这种联系尚未得到详细的研究。因此,我们使用具有手性特异性的分子印迹自支撑膜(MISSM)来研究这种关系,这是专门开发的用于研究门效应的工具。在低至5µM的浓度下,MISSM的扩散渗透率和体积孔隙度对模板化合物(D和l -苯丙氨酸)的存在都很敏感,同时对模板的对映体不敏感。吸附模板量与平衡模板浓度之间的关系符合Langmuir等温线模式,表明了mism中结合位点的热力学均匀性。本研究还表明,暴露于浓度为5µM的模板后,膜中吸附模板的相对浓度仅为3ppm,相对位点占用仅为1%。这些结果表明,栅极效应可以很好地应用于高灵敏度放大器或传感器中。分子印迹聚合物(MIPs)是在聚合过程中通过对靶分子(或模板)的印迹而形成的含有特定结合位点的合成聚合物。MIP层的制备方法简单而经济(1-3)。然而,在这些MIP被用作化学传感装置中的分子识别元件之前,有必要开发一种将MIP上的特定结合事件翻译成电信号的方法。所谓的“门效应”是指由于模板上的特异性结合而导致的MIP层内溶质扩散渗透率的变化,它可以作为一种基于电导学的信号传递机制,利用与模板特异性相互作用相关的MIP膜离子渗透率的变化(4-6)。在这个门类比中,模板对应于钥匙,而允许与模板进行特定重新绑定的MIP位点对应于锁孔。安培法也适用,使用接枝到电极上的薄MIP层,其中可以通过跟踪由氧化还原标记物穿过MIP层的磁导率变化引起的faradic电流变化来检测模板(7-11)。门效应是一种选择性的过程,能够区分
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引用次数: 6
Evaluation of Commercial Solid-Phase Extraction (SPE) Carrier Materials for the Selective Automated Enrichment of Monoterpenoides and their Analysis in Cough Drops, Mouthwashes and Bath Additives by Gas- Chromatography Mass Spectrometry (GC-MS) 商用固相萃取(SPE)载体材料的评价及其对止咳液、漱口水和沐浴添加剂中单萜类化合物的气相色谱质谱分析
Pub Date : 2013-05-30 DOI: 10.2174/1874065001307010012
C. Uhlschmied, C. Krieg, G. Abel, M. Popp, C. Huck, G. Bonn
For sample preparation solid-phase extraction (SPE) became very popular during the 1980s and 1990s. Because method development and its optimization is very time consuming, in most cases C18 is used as standard carrier material without knowing if it possesses the highest efficiency. Therefore detailed studies with different stationary phases are necessary for optimal recovery rates. Due to the resulting high number of samples, automation is of crucial importance. In the study described, the extraction of five monoterpenoids (myrcene, citronellal, p-cymene, menthol and thymol) out of an ethanolic solution was optimized using automated SPE. An automated robotic system, the MEA Personal Purification System ® from PhyNexus™ Inc. (http://www.phynexus.com/), was adopted for high-throughput SPE. It allows the simultaneous automatic extraction of 12 samples in parallel employing different carrier materials. Additionally, critical parameters of automation (speed, volume uptake, skipping of drying step) were variegated to obtain optimal recovery rates. Furthermore, extraction efficiency was compared with manual handling to evaluate its performance. The application of 18 different SPE carrier materials, C18 Hydra, C18 ec, C8, C4, C2, C6H5, C6H11 ec, NO2, CN, NH2/C18, Diol, HR-P, EASY, PA, Florisil ® , Davisil ® , Strata-X ® and cellulose pointed out huge differences in extraction efficiency. Finally, the optimized automated procedure was applied for the enrichment of monoterpenoides and their analysis in selected real samples including cough drops, mouthwash and bath additives by gas-chromatography mass spectrometry (GC-MS).
固相萃取法(SPE)在20世纪80年代和90年代非常流行。由于方法开发和优化非常耗时,在大多数情况下,C18被用作标准载流子材料,而不知道它是否具有最高的效率。因此,有必要对不同固定相进行详细的研究,以获得最佳回收率。由于由此产生的大量样品,自动化是至关重要的。在本研究中,采用自动固相萃取的方法对从乙醇溶液中提取五种单萜类化合物(月桂烯、香茅醛、对伞花烯、薄荷醇和百里香酚)进行了优化。采用PhyNexus™Inc. (http://www.phynexus.com/)的自动化机器人系统MEA Personal Purification system®进行高通量SPE。它允许同时自动提取12个样品平行采用不同的载体材料。此外,自动化的关键参数(速度,体积吸收,跳过干燥步骤)是多样化的,以获得最佳的回收率。并将其提取效率与人工处理进行比较,评价其性能。采用C18 Hydra、C18 ec、C8、C4、C2、C6H5、C6H11 ec、NO2、CN、NH2/C18、Diol、HR-P、EASY、PA、Florisil®、Davisil®、Strata-X®和纤维素等18种不同的SPE载体材料,萃取效率差异较大。最后,将优化后的自动化流程应用于咳药水、漱口水和沐浴液添加剂等实际样品的单萜类化合物富集及气相色谱-质谱(GC-MS)分析。
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引用次数: 6
Evaluation of Ion Exclusion Chromatography as a Method for Determining the Total Carbon Dioxide (TCO2) Concentration in Equine Plasma 离子排斥色谱法测定马血浆中总二氧化碳浓度的评价
Pub Date : 2013-04-19 DOI: 10.2174/1874065001307010006
Chin-Tong Tan
Ion chromatography (IC), utilizing an ion exclusion column coupled to a conductivity detector (CD) via a post column reactor, had been evaluated for quantifying the Total Carbon dioxide (TCO2) level in equine plasma. The concentrations obtained from the analysis of the ASE linearity set correlated with the results obtained when the measurement was done using a Beckman EL-ISE ® instrument (R 2 = 0.998). Equine blood was analyzed using both instruments and the averaged absolute difference for 30 samples was 0.4 mM. The IC method was considered suitable for use to determine the compliance of 36 mM available CO2 per liter of plasma concentration threshold stated in the Malayan Racing Association's (MRA) Regulations.
离子色谱(IC),利用离子排除柱耦合电导率检测器(CD)通过柱后反应器,已经评估了用于定量马血浆中总二氧化碳(TCO2)水平。从ASE线性集分析得到的浓度与使用Beckman EL-ISE®仪器测量得到的结果相关(r2 = 0.998)。使用这两种仪器分析马的血液,30个样本的平均绝对差异为0.4毫米。IC方法被认为适合用于确定每升36毫米可用二氧化碳的血浆浓度阈值是否符合马来亚赛马协会(MRA)规定。
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引用次数: 0
Extractive Spectrophotometric Determination of Niobium (V) Using 3- Hydroxy-2-(4'-Methoxyphenyl)-4-Oxo-4H-l-Benzopyran as a Complexing Agent 3-羟基-2-(4′-甲氧基苯基)-4-氧- 4h - 1 -苯并吡喃萃取分光光度法测定铌(V
Pub Date : 2012-08-24 DOI: 10.2174/1874065001206010039
N. Agnihotri
Hydroxy-2-(4'-methoxyphenyl)-4-oxo-4H-l-benzopyran (HMPB) forms a yellow colored (1:3) complex with niobium (V), which is extracted into dichloromethane from 1.04-4.40 molL -1 HClO4 solution showing an absorption maximum at 398 - 412 nm (figure curve A: 1.0 μg Nb ml -1 versus blank; curve B: blank versus DCM) with Beer's law range of 0-1.3 μg Nb ml -1 , molar absorptivity of 3.764  10 4 L mol -1 cm -1 and the detection limit as 0.036 μg ml -1 . The results are highly reproducible with a standard deviation of ± 0.0037. The linear regression equation is Y=0.399X + 0.006 and the correlation coefficient, r = 1.0004 The method is free from the interference of a large number of analytically important elements. The proposed method handles satisfactorily the analysis of several samples of varying complexity. the niobium concentration range of 0-1.3 μg Nb ml -1 , with a molar absorptivity and Sandell's sensitivity of 3.764  10 4 L mol -1 cm -1 and 0.0025 μg Nb cm -2 , respectively at 405 nm. The linear regression equation of the method is Y=0.399X + 0.006 and the correlation coefficient, r = 1.0004. The method is highly reproducible with a standard deviation of ± 0.0037 and the detection limit 0.036 μg ml -1 . Out of a number of
羟基-2-(4′-甲氧基苯基)-4-氧- 4h -l-苯并吡喃(HMPB)与铌(V)形成黄色(1:3)配合物,从1.04-4.40 mol -1 HClO4溶液中提取到二氯甲烷中,在398 - 412 nm处吸收最大(图曲线a: 1.0 μ Nb ml -1相对空白;曲线B:空白与DCM),比尔定律范围为0 ~ 1.3 μ Nb ml -1,摩尔吸光度为3.76410.4 L mol -1 cm -1,检出限为0.036 μ ml -1。结果重复性好,标准偏差为±0.0037。线性回归方程为Y=0.399X + 0.006,相关系数r = 1.0004,该方法不受大量重要分析元素的干扰。所提出的方法能令人满意地处理不同复杂程度的几种样品的分析。铌的浓度范围为0 ~ 1.3 μg Nb ml -1,在405 nm处的摩尔吸光度和桑德尔灵敏度分别为3.76410.4 L mol -1 cm -1和0.0025 μg Nb cm -2。方法的线性回归方程为Y=0.399X + 0.006,相关系数r = 1.0004。方法重复性好,标准偏差为±0.0037,检出限为0.036 μ ml -1。从…中
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引用次数: 6
Effect of Riboflavin on the Photolysis of Cyanocobolamin in AqueousSolution 核黄素对氰钴胺在水溶液中光解的影响
Pub Date : 2012-08-08 DOI: 10.2174/1874065001206010022
I. Ahmad, Ambreen Hafeez, N. Akhter, F. Vaid, Kiran Qadeer
The kinetics of photolysis of cyanocobalamin (CC) by visible radiation in the presence of riboflavin (RF) at pH 2.0-12.0 has been studied. A specific two-component spectrophotometric method has been used for the simultaneous assay of CC and its photoproduct, hydroxocobalamin (HC), at 525 and 550 nm, without any interference from RF. The apparent first-order rate constants for the photolysis of CC in the presence of 1-5 � 10 -5 M concentrations of RF at pH 2.0- 12.0 range from 0.46 (pH 7.0) to 4.22 � 10 -3 min -1 (pH 3.5). The second-order rate constants for the bimolecular interaction of these vitamins are in the range of 8.66 (pH 7.5) to 45.20 M -1 min -1 (pH 2.0). The rate-pH profile indicates a gradual decrease in rate in the acid pH range followed by the slowest rate and maximum stability around pH 7-8. Thus the cationic form of CC is more susceptible to photolysis compared to the neutral form. The increase in rate in the alkaline region is probably due to the hydrolysis of the amide groups. The kinetic results indicate that RF acts as a sensitizer in the photolysis of CC and in this way promotes the degradation of the molecule. Thus the presence of RF in CC solutions adversely affects the stability of vitamin B12.
研究了核黄素(RF)存在下,在pH 2.0 ~ 12.0范围内可见光光解氰钴胺素(CC)的动力学。采用一种特殊的双组分分光光度法,在525和550 nm处同时测定CC及其光产物羟钴胺素(HC),不受RF干扰。在pH 2.0- 12.0条件下,1-5 μ 10 -5 μ M浓度的RF存在下,CC光解的一级表观速率常数范围为0.46 (pH 7.0)至4.22 μ 10 -3 min -1 (pH 3.5)。这些维生素的双分子相互作用的二级速率常数在8.66 (pH 7.5)至45.20 M -1 min -1 (pH 2.0)之间。速率-pH曲线表明,在酸性pH范围内,速率逐渐降低,其次在pH 7-8附近速率最慢,稳定性最大。因此,阳离子形式的CC比中性形式更容易光解。在碱性区速率的增加可能是由于酰胺基团的水解。动力学结果表明,RF在CC光解过程中起敏化剂的作用,从而促进了分子的降解。因此,CC溶液中RF的存在会对维生素B12的稳定性产生不利影响。
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引用次数: 20
Differential Reaction-Rate Methods in Flow Analysis 流动分析中的微分反应速率方法
Pub Date : 2012-08-08 DOI: 10.2174/1874065001206010028
M. Sasaki
Simultaneous determinations exploiting the rates of reactions of different analytes with a common reagent, often referred to as differential kinetic analysis, is efficiently implemented in the flow analyser. To this end, specific strategies for pumping, commuting and/or detecting have been proposed. This review critically discusses the potentialities, limitations and application ranges of the different flow systems designed to accomplish differential reaction- rate methods, with emphasis to those involving spectrophotometric and luminometric detection.
利用同一试剂对不同分析物的反应速率进行同时测定,通常称为微分动力学分析,可在流动分析仪中有效地实现。为此,提出了泵送、通勤和/或检测的具体策略。本文主要讨论了不同流动系统的潜力、局限性和应用范围,以实现不同的反应速率方法,重点是涉及分光光度法和光度法的检测。
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引用次数: 10
Selective Recognition of Bifunctional Molecules by Synthetic Polymers Prepared by Covalent Molecular Imprinting 共价分子印迹合成聚合物对双功能分子的选择性识别
Pub Date : 2012-05-16 DOI: 10.2174/1874065001206010015
L. Dubey, I. Chianella, I. Dubey, E. Piletska, M. Whitcombe, S. Piletsky
Affinity polymeric adsorbents useful for the separation of compounds which are derivatives of phenols, heterocycles and other compounds containing two polar moieties were prepared by a covalent imprinting approach. Such separation, for example, would facilitate the analytical detection and quantification of each individual compound especially when present in complex sample matrix. The synthetic method, utilised for the preparation of the polymers, is based on co-polymerization of diacrylate esters of hydroxyphenols (catechol, resorcinol and hydroquinone) with an appropriate cross-linker followed by alkaline hydrolysis of the esters and release of the corresponding phenol. The resulting swellable materials contain cavities with adjustable size and orientation of two carboxylic groups suited for the binding of molecules with appropriate size and complementary orientation of polar groups. In contrast to conventional MIPs the synthesized polymers have selectivity not only for the phenolic templates, but also for a group of compounds with suitable orientations of polar functionalities, such as aromatic nitrogen, amino or hydroxyl groups. Polymers demonstrated different affinity to ortho-, meta- and para-isomers of hydroxyphenols, hydroxypyridines and diazine heterocycles.
采用共价印迹法制备了亲和高分子吸附剂,可用于分离酚类衍生物、杂环类化合物和其他含有两极性基团的化合物。这种分离,例如,将有利于分析检测和定量的每一个单独的化合物,特别是当存在于复杂的样品基质。用于制备聚合物的合成方法是将羟基酚(儿茶酚、间苯二酚和对苯二酚)的二丙烯酸酯与适当的交联剂共聚合,然后对酯进行碱性水解并释放相应的苯酚。所得到的可膨胀材料包含具有可调节大小和两个羧基取向的空腔,适合于具有适当大小和极性基团互补取向的分子的结合。与传统的MIPs相比,合成的聚合物不仅对酚模板具有选择性,而且对具有合适极性官能团取向的一组化合物(如芳香氮、氨基或羟基)具有选择性。聚合物对羟基酚、羟基吡啶和嘧啶杂环的邻位异构体、间位异构体和对异构体具有不同的亲和性。
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引用次数: 3
Capillary Gas Chromatography-Mass Spectrometry (CGC-MS) Analysis and Antioxidant Activities of Phenolic and Components of Guarana and Derivatives 毛细管气相色谱-质谱(CGC-MS)分析瓜拉那酚类物质及其衍生物的抗氧化活性
Pub Date : 2012-03-26 DOI: 10.2174/1874065001206010001
E. M. Kuskoski, J. Ríos, J. M. Bueno, R. Fett, A. Troncoso, A. Asuero
The GC-MS analysis of phenolic compounds present in guarana (Paullinia cupana), an important product of the Amazonian forest consumed in supplements or in soft drinks has been the subject of study. The therapeutic properties and possible protective effects reported for guarana and derivative products could be associated with the antioxidant activity of their phenolics content. The purpose of this study is i) to provide molecular structural information about the composition of guarana in phenolics; ii) to ascertain the effect of solvent type on the extraction procedure; and iii) to determine the antioxidant activity of powders, pericarp, pulp seeds, capsules and bar from Paullinia cupana, as oxygen radical absorbance capacity with fluorescein (ORACFL). Three more phenolic compounds, Quercetin, (+)-Catechin and (- )-Epicatechin have been identified in this report as trimethylsilyl (TMS) derivatives. The amount of total phenolics found in plant materials containing guarana varied from 25.10 to 124.99 mg of gallic acid/g dry sample whereas that the antioxidant activity ranged from 441.5 to 1468.3 μmol TEAC/g dry sample. A high correlation was found between the estimated phenolic contents and the TEAC values (r = 0.937, P< 0.01) for all the types of guarana samples tested.
瓜拉那(Paullinia cupana)是亚马逊森林的一种重要产品,用于补充剂或软饮料中,其酚类化合物的GC-MS分析一直是研究的主题。瓜拉那及其衍生物的治疗特性和可能的保护作用可能与其酚类物质的抗氧化活性有关。本研究的目的是:1)提供瓜拉那在酚类物质中组成的分子结构信息;Ii)确定溶剂类型对萃取过程的影响;iii)用荧光素(ORACFL)测定泡莲粉、果皮、果肉种子、胶囊和棒材的抗氧化活性。另外三种酚类化合物槲皮素、(+)-儿茶素和(-)-表儿茶素已被确定为三甲基硅基(TMS)衍生物。含瓜拉那的植物材料中总酚含量为25.10 ~ 124.99 mg /g干样没食子酸,抗氧化活性为441.5 ~ 1468.3 μmol TEAC/g干样。各类型瓜拉那样品中酚类物质含量与TEAC值呈高度相关(r = 0.937, P< 0.01)。
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引用次数: 11
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The open analytical chemistry journal
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