Pub Date : 2018-03-26DOI: 10.5772/INTECHOPEN.73316
Daniel A Monroy, J. M. Bravo, I. Mercado, L. J. V. Gómez
One of the main complications that can present a person with second and third degree burns is the possibility of being infected by opportunistic bacteria or viruses that are present in the environment. Nowadays, the majority of the burn injuries are treated with con- ventional gauze, which involves a high probability of infection and pain for the patient being treated with this method. In order to obtain low-cost scaffolds, natural and abun - dant polymers were used such as gelatin (GEL) and collagen (COL). The GEL functions as a base scaffold, stable and flexible, and also biocompatible because it is a byproduct of the partial hydrolysis of COL, which is an indispensable component for the stability of the cell membrane and it is present in great extent in the human epithelium. nonwoven fabric, which was pre-grafted with acrylic acid or N-isopropyl acrylamide to con-struct a durable wound sandwich liner membrane with high water absorption, easy removal, and antibacterial activity in an animal skin model. The results indicated that tissue immobilized with N-isopropyl acrylamide and COL/chitosan/PP/N-isopropyl acrylamide-COL/chito-san) showed a better healing effect than COL/chitosan immobilized polypropylene tissue. The poly (propylene)/N-isopropyl acrylamide/COL/chitosan-treated wound showed an excellent remodeling effect on histological examination with respect to the construction of the vein, epi dermis, and dermis at 21 days post-cutaneous lesion. The water absorption values and water diffusion coefficient for polypropylene/N-isopropyl acrylamide/COL/chitosan were higher than those of polypropylene /acrylic acid/COL/chitosan under a weight-volume ratio of COL/ chitosan. Both polypropylene/N-isopropyl acrylamide/COL/chitosan and poly (propylene)/ acrylic acid/COL/chitosan showed antibacterial activity [20].
二度和三度烧伤的主要并发症之一是可能被环境中存在的机会性细菌或病毒感染。目前，大多数烧伤都是用传统纱布治疗的，这种方法对患者来说感染和疼痛的可能性很大。为了获得低成本的支架，使用了天然丰富的聚合物如明胶(GEL)和胶原蛋白(COL)。凝胶的功能是作为基础支架，稳定而灵活，并且具有生物相容性，因为它是COL部分水解的副产品，COL是细胞膜稳定性不可或缺的成分，并且在很大程度上存在于人上皮中。在动物皮肤模型中，采用丙烯酸或n-异丙基丙烯酰胺预接枝非织造布，构建具有高吸水性、易去除和抗菌活性的耐用伤口夹层内衬膜。结果表明，n -异丙基丙烯酰胺和COL/壳聚糖/PP/ n -异丙基丙烯酰胺-COL/壳聚糖)固定的组织愈合效果优于COL/壳聚糖固定的聚丙烯组织。聚丙烯/ n-异丙基丙烯酰胺/COL/壳聚糖处理的创面在皮肤损伤后21天的静脉、表皮和真皮结构的组织学检查中显示出良好的重塑效果。在COL/壳聚糖的体积比下，聚丙烯/ n -异丙基丙烯酰胺/COL/壳聚糖的吸水率和水扩散系数均高于聚丙烯/丙烯酸/COL/壳聚糖。聚丙烯/ n -异丙基丙烯酰胺/COL/壳聚糖和聚丙烯/丙烯酸/COL/壳聚糖均表现出抗菌活性[20]。
{"title":"Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering","authors":"Daniel A Monroy, J. M. Bravo, I. Mercado, L. J. V. Gómez","doi":"10.5772/INTECHOPEN.73316","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.73316","url":null,"abstract":"One of the main complications that can present a person with second and third degree burns is the possibility of being infected by opportunistic bacteria or viruses that are present in the environment. Nowadays, the majority of the burn injuries are treated with con- ventional gauze, which involves a high probability of infection and pain for the patient being treated with this method. In order to obtain low-cost scaffolds, natural and abun - dant polymers were used such as gelatin (GEL) and collagen (COL). The GEL functions as a base scaffold, stable and flexible, and also biocompatible because it is a byproduct of the partial hydrolysis of COL, which is an indispensable component for the stability of the cell membrane and it is present in great extent in the human epithelium. nonwoven fabric, which was pre-grafted with acrylic acid or N-isopropyl acrylamide to con-struct a durable wound sandwich liner membrane with high water absorption, easy removal, and antibacterial activity in an animal skin model. The results indicated that tissue immobilized with N-isopropyl acrylamide and COL/chitosan/PP/N-isopropyl acrylamide-COL/chito-san) showed a better healing effect than COL/chitosan immobilized polypropylene tissue. The poly (propylene)/N-isopropyl acrylamide/COL/chitosan-treated wound showed an excellent remodeling effect on histological examination with respect to the construction of the vein, epi dermis, and dermis at 21 days post-cutaneous lesion. The water absorption values and water diffusion coefficient for polypropylene/N-isopropyl acrylamide/COL/chitosan were higher than those of polypropylene /acrylic acid/COL/chitosan under a weight-volume ratio of COL/ chitosan. Both polypropylene/N-isopropyl acrylamide/COL/chitosan and poly (propylene)/ acrylic acid/COL/chitosan showed antibacterial activity [20].","PeriodicalId":90802,"journal":{"name":"Bone and tissue regeneration insights","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76959009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01Epub Date: 2016-06-20DOI: 10.4137/BTRI.S19954
Sriniwas Sriram, Jennifer A Tran, James D Zieske
Scarring remains a serious complication of the wound healing process that can lead to the formation of excessive fibrous connective tissue in an organ or tissue leading to pain and loss of function. This process is mainly regulated by Transforming growth factor β1 (TGF-β1), which binds to receptors and induces its downstream mediator, Connective tissue growth factor (CTGF). The number of drugs targeting CTGF for treating scars has been on the rise in the past few years. The purpose of this article is to suggest the possibility of using cornea as a model for testing anti-CTGF therapies for scarring.
{"title":"Cornea As a Model for Testing CTGF-Based Antiscarring Drugs.","authors":"Sriniwas Sriram, Jennifer A Tran, James D Zieske","doi":"10.4137/BTRI.S19954","DOIUrl":"https://doi.org/10.4137/BTRI.S19954","url":null,"abstract":"<p><p>Scarring remains a serious complication of the wound healing process that can lead to the formation of excessive fibrous connective tissue in an organ or tissue leading to pain and loss of function. This process is mainly regulated by Transforming growth factor β1 (TGF-β1), which binds to receptors and induces its downstream mediator, Connective tissue growth factor (CTGF). The number of drugs targeting CTGF for treating scars has been on the rise in the past few years. The purpose of this article is to suggest the possibility of using cornea as a model for testing anti-CTGF therapies for scarring.</p>","PeriodicalId":90802,"journal":{"name":"Bone and tissue regeneration insights","volume":"7 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BTRI.S19954","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36188223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Modern medicine faces a growing crisis as demand for organ transplantations continues to far outstrip supply. By stimulating the body's own repair mechanisms, regenerative medicine aims to reduce demand for organs, while the closely related field of tissue engineering promises to deliver "off-the-self" organs grown from patients' own stem cells to improve supply. To deliver on these promises, we must have reliable means of generating complex tissues. Thus far, the majority of successful tissue engineering approaches have relied on macroporous scaffolds to provide cells with both mechanical support and differentiative cues. In order to engineer complex tissues, greater attention must be paid to nanoscale cues present in a cell's microenvironment. As the extracellular matrix is capable of driving complexity during development, it must be understood and reproduced in order to recapitulate complexity in engineered tissues. This review will summarize current progress in engineering complex tissue through the integration of nanocomposites and biomimetic scaffolds.
{"title":"Nanotechnology in the Regeneration of Complex Tissues.","authors":"John W Cassidy","doi":"10.4137/BTRI.S12331","DOIUrl":"https://doi.org/10.4137/BTRI.S12331","url":null,"abstract":"<p><p>Modern medicine faces a growing crisis as demand for organ transplantations continues to far outstrip supply. By stimulating the body's own repair mechanisms, regenerative medicine aims to reduce demand for organs, while the closely related field of tissue engineering promises to deliver \"off-the-self\" organs grown from patients' own stem cells to improve supply. To deliver on these promises, we must have reliable means of generating complex tissues. Thus far, the majority of successful tissue engineering approaches have relied on macroporous scaffolds to provide cells with both mechanical support and differentiative cues. In order to engineer complex tissues, greater attention must be paid to nanoscale cues present in a cell's microenvironment. As the extracellular matrix is capable of driving complexity during development, it must be understood and reproduced in order to recapitulate complexity in engineered tissues. This review will summarize current progress in engineering complex tissue through the integration of nanocomposites and biomimetic scaffolds.</p>","PeriodicalId":90802,"journal":{"name":"Bone and tissue regeneration insights","volume":"5 ","pages":"25-35"},"PeriodicalIF":0.0,"publicationDate":"2014-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BTRI.S12331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33408639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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