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Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems 两水相体系中肽标记角质酶的提取
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1011190713820
A. Rodenbrock, K. Selber, M. Egmond, M. Kula
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引用次数: 27
Recovery of mouse endostatin produced by Pichia pastoris using expanded bed adsorption 膨胀床吸附法回收毕赤酵母生产的小鼠内皮抑素
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1008133914854
L. Trinh, S. Noronha, M. Fannon, J. Shiloach
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引用次数: 20
Optimization of bovine serum albumin partition coefficient in aqueous two-phase systems 两水相体系中牛血清白蛋白分配系数的优化
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1011194324047
U. Gündüz
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引用次数: 10
The effect of demulsifiers on lysozyme extraction from hen egg white using reverse micelles. 破乳剂对反胶束法提取蛋清溶菌酶的影响。
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/a:1008183828223
S Jarudilokkul, E Paulsen, D C Stuckey

The liquid-liquid extraction of protein from buffered aqueous phases using reverse micelles (RM) has been extensively researched from a fundamental point of view. However, very little effort has been expended at scaling up this process for the extraction of real fermentation broth. When real broths are used with reverse micellar phases there are major problems with emulsion formation. In this study the effect of a variety of demulsifiers on lysozyme extraction was evaluated in terms of their influence on the separating properties of the emulsion, water content (Wo), and, extraction yield and kinetics from both buffer and hen egg white. In addition, the use of a low shear contactor (a Graesser or 'raining bucket') was assessed in terms of its suitability as a RM contactor. It was found that most of the demulsifiers reduced the settling time of the emulsion, and enhanced the yield and kinetics of lysozyme extraction from hen egg white. It was hypothesised that this was due to the demulsifier displacing the lysozyme from the interface and preventing the protein unfolding and precipitating. This effect was found to depend on both the generic type of demulsifier, and its concentration.

利用反胶束(RM)从缓冲水相中提取蛋白质的液液萃取技术已经从基础的角度得到了广泛的研究。然而，很少的努力已经花费在扩大这一过程，以提取真正的发酵液。当真正的肉汤与反胶束相使用时，乳剂形成的主要问题。本研究考察了不同破乳剂对溶菌酶提取的影响，考察了不同破乳剂对乳浊液和蛋清的分离性能、水含量(Wo)、提取率和动力学的影响。此外，对低剪切接触器(Graesser或“雨桶”)作为RM接触器的适用性进行了评估。结果表明，大多数破乳剂均能缩短乳状液的沉淀时间，提高蛋清溶菌酶的提取率和动力学。据推测，这是由于破乳剂从界面上取代了溶菌酶，阻止了蛋白质的展开和沉淀。发现这种效果取决于破乳剂的一般类型及其浓度。
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引用次数: 17
One step purification of peanut phospholipase D by precipitation with alginate. 海藻酸盐沉淀法一步纯化花生磷脂酶D。
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/a:1008121005974
S Sharma, A Sharma, M N Gupta

A simple titrimetric assay with soybean lecithin has been used for screening phospholipase D activity from some plant sources, viz. peanut, wheat germ, cabbage and carrot. The enzyme from peanut has been purified by binding to alginate which is a water soluble polymer. The purification consisted of co-precipitation of enzyme with alginate upon addition of 0.06 M Ca++. The enzyme was eluted from the polymer using 0.2 M sodium chloride. The activity recovery was 61% with 34 fold purification.

大豆卵磷脂的简单滴定法已被用于筛选一些植物来源的磷脂酶D活性，如花生、小麦胚芽、卷心菜和胡萝卜。通过与海藻酸盐(一种水溶性聚合物)结合纯化花生酶。纯化是在添加0.06 M Ca++的情况下，酶与海藻酸盐共沉淀。用0.2 M氯化钠从聚合物中洗脱酶。经34倍纯化，活性回收率为61%。
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引用次数: 21
Modeling of direct recovery of lactic acid from whole broths by ion exchange adsorption 离子交换吸附法直接回收全肉汤中乳酸的模拟
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1011165810823
A. V. Sosa, J. Ochoa, N. Perotti
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引用次数: 17
Purification of a `double-headed' inhibitor of α-amylase/Proteinase K from wheat germ by expanded bed chromatography 扩大床层析法纯化小麦胚芽α-淀粉酶/蛋白酶K双头抑制剂
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1008120025526
I. Roy, M. N. Gupta
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引用次数: 18
Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch 盐和表面活性剂对热分离环氧乙烷/环氧丙烷无规共聚物和羟丙基淀粉双水相体系中茄灰镰刀菌表皮酶分配的影响
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1008132108117
Maria Teresa Cunha, J. Cabral, F. Tjerneld, M. Raquel Aires-Barros
{"title":"Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch","authors":"Maria Teresa Cunha, J. Cabral, F. Tjerneld, M. Raquel Aires-Barros","doi":"10.1023/A:1008132108117","DOIUrl":"https://doi.org/10.1023/A:1008132108117","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"271 1","pages":"203-209"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77574547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Investigation of enhancement of two processes, sedimentation and conjugation, when bacteria are concentrated in ultrasonic standing waves 超声波驻波对细菌沉降和共轭两种过程的强化研究
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1011181431742
C. M. Cousins, Jonas Melin, W. Albert Venables, W. Terence Coakley
{"title":"Investigation of enhancement of two processes, sedimentation and conjugation, when bacteria are concentrated in ultrasonic standing waves","authors":"C. M. Cousins, Jonas Melin, W. Albert Venables, W. Terence Coakley","doi":"10.1023/A:1011181431742","DOIUrl":"https://doi.org/10.1023/A:1011181431742","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"80 1","pages":"343-349"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90502789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Eicosapentaenoic and arachidonic acids purification from the red microalga Porphyridium cruentum 红微藻卟啉的二十碳五烯酸和花生四烯酸的纯化
Bioseparation
Pub Date : 2000-01-01 DOI: 10.1023/A:1011124624001
J. Guil‐Guerrero, E. Belarbi, M. M. Rebolloso-Fuentes
{"title":"Eicosapentaenoic and arachidonic acids purification from the red microalga Porphyridium cruentum","authors":"J. Guil‐Guerrero, E. Belarbi, M. M. Rebolloso-Fuentes","doi":"10.1023/A:1011124624001","DOIUrl":"https://doi.org/10.1023/A:1011124624001","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"5 1","pages":"299-306"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87062195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 61
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