Background: Blood testing remains an essential component of diagnosis and prevention of disease. Phlebotomists, Laboratory Infrastructure, Storage, Collection, and Transport are required for Current Methods of Blood Collection. Menstrual Blood has many similar characteristics to circulating blood but has not previously been assessed as a Potential Diagnostic Resource. Objective: To assess if biomarkers derived from menstrual blood correlate with systemic blood. Study design: This was a prospective, observational pilot study of healthy reproductive-aged women. We chose a panel of 9 Biomarkers, used in preventative health assessments and for following clinical conditions, and compared systemic and menstrual blood levels. Results: Eighty-Four volunteers were screened over two months; 35 provided a menstrual and serum sample, of which 20 had a sample adequate for analysis. Overall, the correlation was observed, particularly for 7 Biomarkers, with no statistically significant differences between the mean menstrual and serum values. These include cholesterol (P for differences in means=0.89, R2 for correlation=0.89), Creatinine (P=0.32, R2=0.94), HSCRP (P=0.89, R2=0.99), LDL (P=0.21, R2=0.84), Triglycerides (P=0.45, R2=0.89), Hba1c (P=0.54, R2=0.80), and HDL (P=0.33, R2=0.77). One biomarker, FSH (P<0.001, R2=0.97), was less directly comparable to systemic blood, but a linear relationship was recognized suggesting that the correlation could be mathematically derived, and therefore diagnostic utility is possible. Conclusion: Based on our results, menstrual blood can reliably estimate levels of several biomarkers and may be a promising option for noninvasive collection of blood for diagnosis and health monitoring. Larger trials are needed to confirm these findings. of disorders were present in menstrual fluid [1]. These disorders include endometriosis, breast, Cervical, Ovarian and Endometrial Cancer. Several other studies have detected the presence of Human Papilloma Virus (HPV) in menstrual blood implying that it may be useful for non-invasive screening for cervical cancer or pre-cancer [2,3]. Furthermore, menstrual blood has been studied for screening or diagnosis of Sexually Transmitted Infections (STIS). Alary M, et al. explored the potential of using vaginal fluid collected in menstrual pads for Chlamydia detection. This proved to have sensitivity and specificity equivalent to, or even higher than, existing methods, including vaginal swabs or urine samples [4]. These data combine to provide support for the potential utility of menstrual blood-based testing as a non-invasive alternative blood source for diagnostic or therapeutic analysis. Despite this apparent potential, menstrual blood remains a relatively uninvestigated area for diagnostics; no published literature explores the correlation of systemic versus menstrual-based blood for assessing biomarkers relative to indicators of health or disease. Introduction Whole blood or Serum (Systemic Blood)
背景:血液检测仍然是疾病诊断和预防的重要组成部分。目前的血液采集方法需要抽血师、实验室基础设施、储存、采集和运输。经血与循环血液有许多相似的特征，但以前没有被评估为潜在的诊断资源。目的:评估从经血中提取的生物标志物是否与全身血液相关。研究设计:这是一项对健康育龄妇女的前瞻性、观察性先导研究。我们选择了一组9个生物标志物，用于预防性健康评估和后续临床状况，并比较了全身和经血水平。结果:在两个月的时间里，84名志愿者被筛选;35个国家提供了月经和血清样本，其中20个国家有足够的样本供分析。总体而言，观察到相关性，特别是7个生物标志物，平均月经值和血清值之间没有统计学上的显着差异。这些指标包括胆固醇(P=均值差异=0.89,R2=0.89)、肌酐(P=0.32, R2=0.94)、HSCRP (P=0.89, R2=0.99)、LDL (P=0.21, R2=0.84)、甘油三酯(P=0.45, R2=0.89)、糖化血红蛋白(P=0.54, R2=0.80)和高密度脂蛋白(P=0.33, R2=0.77)。一种生物标志物，FSH (P<0.001, R2=0.97)，与全身血液的直接可比性较低，但认识到线性关系，表明相关性可以从数学上推导出来，因此诊断实用是可能的。结论:根据我们的研究结果，经血可以可靠地估计几种生物标志物的水平，并且可能是用于诊断和健康监测的无创采血的有前途的选择。需要更大规模的试验来证实这些发现。在月经液中出现紊乱。这些疾病包括子宫内膜异位症、乳腺癌、子宫癌、卵巢癌和子宫内膜癌。其他几项研究已经在经血中检测到人乳头瘤病毒(HPV)的存在，这意味着它可能对宫颈癌或癌前病变的非侵入性筛查有用[2,3]。此外，经血已被研究用于筛查或诊断性传播感染(STIS)。Alary M等人探讨了使用经垫收集的阴道液进行衣原体检测的潜力。事实证明，这种方法的灵敏度和特异性与包括阴道拭子或尿液样本在内的现有方法相当，甚至更高。这些数据结合起来，为经血检测作为诊断或治疗分析的非侵入性替代血源的潜在效用提供了支持。尽管有这种明显的潜力，经血仍然是一个相对未被研究的诊断领域;没有发表的文献探讨全身血液与月经血液在评估与健康或疾病指标相关的生物标志物方面的相关性。全血或血清(全身血液)通常用于诊断或监测许多疾病。然而，获取标本是一个侵入性的过程，需要医疗援助。它可能会带来不便、昂贵、痛苦和焦虑。大多数育龄妇女有规律地来月经，虽然经血与全身血液有许多相同的特征，但它还没有经过严格的临床研究以用于诊断和治疗目的。经血由三种不同的体液组成:全血、阴道分泌液以及月经期间子宫内膜和子宫颈分泌后期的细胞和液体。分子蛋白质组学研究显示与全身血液有相当大的相关性;然而，经血也含有额外的液体[1]。具体来说，与全身血液相比，经血中至少可以检测到385种额外的蛋白质。2012年，Siegel D等人首次定义了经血的蛋白质组学，并发现了几种广泛的生物标志物，如Sci Forschen O、H U B、H U B、H U B、H U B、H U B、c c和c c。引文:Naseri S, Lerma K, Blumenthal PD(2019)血清与经血用于诊断目的的比较评估:a Pilot Study。为了评估这一点，我们进行了一项概念验证研究，将经血与全身血液进行比较。我们假设，如果经血生物标志物与全身血液相关，这种替代检测方法可能有潜力成为一种方便、无创和成本效益高的血液分析方法，用于诊断和治疗。如果发现可靠，这种方法可以增加月经妇女早期疾病检测和定期健康监测的机会。材料和方法本研究是一项前瞻性、观察性、先导性的健康育龄妇女研究。 背景:血液检测仍然是疾病诊断和预防的重要组成部分。目前的血液采集方法需要抽血师、实验室基础设施、储存、采集和运输。经血与循环血液有许多相似的特征，但以前没有被评估为潜在的诊断资源。目的:评估从经血中提取的生物标志物是否与全身血液相关。研究设计:这是一项对健康育龄妇女的前瞻性、观察性先导研究。我们选择了一组9个生物标志物，用于预防性健康评估和后续临床状况，并比较了全身和经血水平。结果:在两个月的时间里，84名志愿者被筛选;35个国家提供了月经和血清样本，其中20个国家有足够的样本供分析。总体而言，观察到相关性，特别是7个生物标志物，平均月经值和血清值之间没有统计学上的显着差异。这些指标包括胆固醇(P=均值差异=0.89,R2=0.89)、肌酐(P=0.32, R2=0.94)、HSCRP (P=0.89, R2=0.99)、LDL (P=0.21, R2=0.84)、甘油三酯(P=0.45, R2=0.89)、糖化血红蛋白(P=0.54, R2=0.80)和高密度脂蛋白(P=0.33, R2=0.77)。一种生物标志物，FSH (P<0.001, R2=0.97)，与全身血液的直接可比性较低，但认识到线性关系，表明相关性可以从数学上推导出来，因此诊断实用是可能的。结论:根据我们的研究结果，经血可以可靠地估计几种生物标志物的水平，并且可能是用于诊断和健康监测的无创采血的有前途的选择。需要更大规模的试验来证实这些发现。在月经液中出现紊乱。这些疾病包括子宫内膜异位症、乳腺癌、子宫癌、卵巢癌和子宫内膜癌。其他几项研究已经在经血中检测到人乳头瘤病毒(HPV)的存在，这意味着它可能对宫颈癌或癌前病变的非侵入性筛查有用[2,3]。此外，经血已被研究用于筛查或诊断性传播感染(STIS)。Alary M等人探讨了使用经垫收集的阴道液进行衣原体检测的潜力。事实证明，这种方法的灵敏度和特异性与包括阴道拭子或尿液样本在内的现有方法相当，甚至更高。这些数据结合起来，为经血检测作为诊断或治疗分析的非侵入性替代血源的潜在效用提供了支持。尽管有这种明显的潜力，经血仍然是一个相对未被研究的诊断领域;没有发表的文献探讨全身血液与月经血液在评估与健康或疾病指标相关的生物标志物方面的相关性。全血或血清(全身血液)通常用于诊断或监测许多疾病。然而，获取标本是一个侵入性的过程，需要医疗援助。它可能会带来不便、昂贵、痛苦和焦虑。大多数育龄妇女有规律地来月经，虽然经血与全身血液有许多相同的特征，但它还没有经过严格的临床研究以用于诊断和治疗目的。经血由三种不同的体液组成:全血、阴道分泌液以及月经期间子宫内膜和子宫颈分泌后期的细胞和液体。分子蛋白质组学研究显示与全身血液有相当大的相关性;然而，经血也含有额外的液体[1]。具体来说，与全身血液相比，经血中至少可以检测到385种额外的蛋白质。2012年，Siegel D等人首次定义了经血的蛋白质组学，并发现了几种广泛的生物标志物，如Sci Forschen O、H U B、H U B、H U B、H U B、H U B、c c和c c。引文:Naseri S, Lerma K, Blumenthal PD(2019)血清与经血用于诊断目的的比较评估:a Pilot Study。为了评估这一点，我们进行了一项概念验证研究，将经血与全身血液进行比较。我们假设，如果经血生物标志物与全身血液相关，这种替代检测方法可能有潜力成为一种方便、无创和成本效益高的血液分析方法，用于诊断和治疗。如果发现可
{"title":"Comparative Assessment of Serum versus Menstrual Blood for Diagnostic Purposes: A Pilot Study","authors":"Naseri S, Lerma K, Blumenthal Pd","doi":"10.16966/2572-9578.130","DOIUrl":"https://doi.org/10.16966/2572-9578.130","url":null,"abstract":"Background: Blood testing remains an essential component of diagnosis and prevention of disease. Phlebotomists, Laboratory Infrastructure, Storage, Collection, and Transport are required for Current Methods of Blood Collection. Menstrual Blood has many similar characteristics to circulating blood but has not previously been assessed as a Potential Diagnostic Resource. Objective: To assess if biomarkers derived from menstrual blood correlate with systemic blood. Study design: This was a prospective, observational pilot study of healthy reproductive-aged women. We chose a panel of 9 Biomarkers, used in preventative health assessments and for following clinical conditions, and compared systemic and menstrual blood levels. Results: Eighty-Four volunteers were screened over two months; 35 provided a menstrual and serum sample, of which 20 had a sample adequate for analysis. Overall, the correlation was observed, particularly for 7 Biomarkers, with no statistically significant differences between the mean menstrual and serum values. These include cholesterol (P for differences in means=0.89, R2 for correlation=0.89), Creatinine (P=0.32, R2=0.94), HSCRP (P=0.89, R2=0.99), LDL (P=0.21, R2=0.84), Triglycerides (P=0.45, R2=0.89), Hba1c (P=0.54, R2=0.80), and HDL (P=0.33, R2=0.77). One biomarker, FSH (P<0.001, R2=0.97), was less directly comparable to systemic blood, but a linear relationship was recognized suggesting that the correlation could be mathematically derived, and therefore diagnostic utility is possible. Conclusion: Based on our results, menstrual blood can reliably estimate levels of several biomarkers and may be a promising option for noninvasive collection of blood for diagnosis and health monitoring. Larger trials are needed to confirm these findings. of disorders were present in menstrual fluid [1]. These disorders include endometriosis, breast, Cervical, Ovarian and Endometrial Cancer. Several other studies have detected the presence of Human Papilloma Virus (HPV) in menstrual blood implying that it may be useful for non-invasive screening for cervical cancer or pre-cancer [2,3]. Furthermore, menstrual blood has been studied for screening or diagnosis of Sexually Transmitted Infections (STIS). Alary M, et al. explored the potential of using vaginal fluid collected in menstrual pads for Chlamydia detection. This proved to have sensitivity and specificity equivalent to, or even higher than, existing methods, including vaginal swabs or urine samples [4]. These data combine to provide support for the potential utility of menstrual blood-based testing as a non-invasive alternative blood source for diagnostic or therapeutic analysis. Despite this apparent potential, menstrual blood remains a relatively uninvestigated area for diagnostics; no published literature explores the correlation of systemic versus menstrual-based blood for assessing biomarkers relative to indicators of health or disease. Introduction Whole blood or Serum (Systemic Blood)","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ap, Pazos Cp, Penton Crc, D. Ap, Perez Lr, L DominguezPlain
Castleman’s disease is a very rare lymphoproliferative disorder. There are two Clinical forms: the unicentric, which is usually benign, and the multicentric progressive course. There are three described histological patterns: hyali neovascular of unicentric presentation, of plasma cells of multicentric presentation and the mixed one. The aim of this paper is to present a clinical case of multicentric Castleman’s disease of hyaline vascular histology very little described in the literature. The patient started with asthenia, anorexia, profuse nocturnal sweating, weight loss, generalized lymphadenopathy, splenomegaly and respiratory episodes. At physical examination: multiple cervical, axillary and bilateral inguinal adenopathies. The diagnosis was made with the histopathological study of the resected ganglion, the evolution after the treatment has been favorable. Because there are no specific clinical and radiographic features, a high degree of diagnostic suspicion and the experience of the pathologist are required to diagnose this rare disease.
{"title":"Castleman’s Disease about a Case","authors":"D. Ap, Pazos Cp, Penton Crc, D. Ap, Perez Lr, L DominguezPlain","doi":"10.16966/2572-9578.131","DOIUrl":"https://doi.org/10.16966/2572-9578.131","url":null,"abstract":"Castleman’s disease is a very rare lymphoproliferative disorder. There are two Clinical forms: the unicentric, which is usually benign, and the multicentric progressive course. There are three described histological patterns: hyali neovascular of unicentric presentation, of plasma cells of multicentric presentation and the mixed one. The aim of this paper is to present a clinical case of multicentric Castleman’s disease of hyaline vascular histology very little described in the literature. The patient started with asthenia, anorexia, profuse nocturnal sweating, weight loss, generalized lymphadenopathy, splenomegaly and respiratory episodes. At physical examination: multiple cervical, axillary and bilateral inguinal adenopathies. The diagnosis was made with the histopathological study of the resected ganglion, the evolution after the treatment has been favorable. Because there are no specific clinical and radiographic features, a high degree of diagnostic suspicion and the experience of the pathologist are required to diagnose this rare disease.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"286 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Electrogastrography (EGG) is a non-invasive method for the diagnosis of gastric electrical activity. This study used an EGG to analyze the gastric activity in children with Chronic Abdominal Pain (CAP). Methods: We conducted a retrospective analysis of patients with CAP who underwent EGG at the first affiliated Hospital of Anhui Medical University. Multichannel EGG with 8-minutes recordings preprandially and postprandially was done in children with CAP. Computerized spectral analysis methods were used to compute various EGG parameters. Results: Chronic superficial gastritis and Functional Dyspepsia (FD) were very common among 96 patients with CAP. The dominant power and waveform response area of EGG was significantly lower in FD patients and chronic superficial gastritis in both preprandial and postprandial states. DP, WRA and power ratio was obviously higher in patients with superficial gastritis, duodenitis and reflux esophagitis in their postprandial states. Conclusions: Gastric myoelectrical activity abnormalities were observed in CAP patients and different abnormality of EGG parameters was shown in different groups. EGG is an effective, reliable and non-invasive method in the diagnosis of CAP subgroups.
{"title":"Electrogastrogram Analysis in Children with Chronic Abdominal Pain","authors":"Yu Lp, C. Jie, Zhou Ql, G. Ming","doi":"10.16966/2572-9578.125","DOIUrl":"https://doi.org/10.16966/2572-9578.125","url":null,"abstract":"Objective: Electrogastrography (EGG) is a non-invasive method for the diagnosis of gastric electrical activity. This study used an EGG to analyze the gastric activity in children with Chronic Abdominal Pain (CAP). Methods: We conducted a retrospective analysis of patients with CAP who underwent EGG at the first affiliated Hospital of Anhui Medical University. Multichannel EGG with 8-minutes recordings preprandially and postprandially was done in children with CAP. Computerized spectral analysis methods were used to compute various EGG parameters. Results: Chronic superficial gastritis and Functional Dyspepsia (FD) were very common among 96 patients with CAP. The dominant power and waveform response area of EGG was significantly lower in FD patients and chronic superficial gastritis in both preprandial and postprandial states. DP, WRA and power ratio was obviously higher in patients with superficial gastritis, duodenitis and reflux esophagitis in their postprandial states. Conclusions: Gastric myoelectrical activity abnormalities were observed in CAP patients and different abnormality of EGG parameters was shown in different groups. EGG is an effective, reliable and non-invasive method in the diagnosis of CAP subgroups.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Hyperthyroidism is an important metabolic disorder associated with multiple biochemical abnormalities that have direct impact on lipid metabolism. The aim of the current study is to assess lipid profile pattern in patients with hyperthyroidism attending Aminu Kano Teaching Hospital, Kano. Methods : A case control study conducted during the period of November 2015 to December 2017 among 100 hyperthyroidism patients before treatment and 100 apparently healthy individuals as controls with age between 18-70 years. Sixty eight of the patients were females while thirty two were males. Fasting blood was collected from each individual to determine serum TSH, T3 and T4 with quantitative ELISA assay technique, Product of ACCU BIND Inc., USA. Lipid profile was measured by an enzymatic spectrophotometric method and precipitation enzymatic method for HDL-C. SPSS software package version 20 was used for the analysis of data. Results : The results of the present study showed that the mean values of T3, T4, TG and TG/HDL-C ratio were significantly higher (p<0.05) in patients with hyperthyroidism than the control group while TSH, T4/T3 ratio TC, HDL-C and LDL-C were significantly lower (p<0.05) in patients with hyperthyroidism than the control group. There were significant (p<0.05) and positive correlations between TSH and TC, HDL-C and LDL-C respectively in the patients. There was no significant (p<0.05) correlation between TSH and TG in the patients. There were no significant correlations in the control group of the respective analytes. Conclusion : Our results revealed that hyperthyroidism was associated with decrease total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and slightly increased triglycerides. LDL and negative correlation, but not significant between TSH a pond TGs. These findings are similar with the report of Shashi A and Sharma N [31]. They reported positive correlations between serum TSH and TC, TSH and LDL, TSH and HDL, but not with TSH and TG. On the other positive correlations changes in resting subsequent loss of disparities findings compared previous findings differences in the research design and the subjects used in the studies.
背景:甲亢是一种重要的代谢性疾病，与多种生化异常相关，直接影响脂质代谢。本研究的目的是评估在卡诺县明野教学医院就诊的甲亢患者的脂质谱模式。方法:于2015年11月至2017年12月对100例治疗前甲状腺功能亢进患者和100例18-70岁的表面健康者作为对照进行病例对照研究。68名患者为女性，32名患者为男性。采用美国ACCU BIND公司产品，采集空腹血，测定血清TSH、T3、T4。脂质谱测定采用酶光度法和沉淀酶法测定HDL-C。采用SPSS软件包第20版对数据进行分析。结果:本研究结果显示，甲亢患者T3、T4、TG、TG/HDL-C均值显著高于对照组(p<0.05)， TSH、T4/T3比值TC、HDL-C、LDL-C均值显著低于对照组(p<0.05)。患者TSH与TC、HDL-C、LDL-C呈显著正相关(p<0.05)。患者TSH与TG之间无显著相关性(p<0.05)。在各自分析物的对照组中没有显著的相关性。结论:甲状腺机能亢进与总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇降低及甘油三酯轻度升高有关。LDL与TSH呈负相关，但TSH与TGs之间无显著相关性。这些发现与Shashi A和Sharma N bbb的报告相似。他们报告了血清TSH与TC、TSH与LDL、TSH与HDL呈正相关，但与TSH与TG无正相关。在其他正相关方面，静息后差异损失的变化与先前研究设计和研究对象的差异进行了比较。
{"title":"Assessment of Lipid Profile Pattern in Patients with Hyperthyroidism in a Tertiary Hospital in Kano, North Western Nigeria","authors":"Yahaya Is, Adimabua Op, Ocheni Ap, Adamu Gb","doi":"10.16966/2572-9578.127","DOIUrl":"https://doi.org/10.16966/2572-9578.127","url":null,"abstract":"Background : Hyperthyroidism is an important metabolic disorder associated with multiple biochemical abnormalities that have direct impact on lipid metabolism. The aim of the current study is to assess lipid profile pattern in patients with hyperthyroidism attending Aminu Kano Teaching Hospital, Kano. Methods : A case control study conducted during the period of November 2015 to December 2017 among 100 hyperthyroidism patients before treatment and 100 apparently healthy individuals as controls with age between 18-70 years. Sixty eight of the patients were females while thirty two were males. Fasting blood was collected from each individual to determine serum TSH, T3 and T4 with quantitative ELISA assay technique, Product of ACCU BIND Inc., USA. Lipid profile was measured by an enzymatic spectrophotometric method and precipitation enzymatic method for HDL-C. SPSS software package version 20 was used for the analysis of data. Results : The results of the present study showed that the mean values of T3, T4, TG and TG/HDL-C ratio were significantly higher (p<0.05) in patients with hyperthyroidism than the control group while TSH, T4/T3 ratio TC, HDL-C and LDL-C were significantly lower (p<0.05) in patients with hyperthyroidism than the control group. There were significant (p<0.05) and positive correlations between TSH and TC, HDL-C and LDL-C respectively in the patients. There was no significant (p<0.05) correlation between TSH and TG in the patients. There were no significant correlations in the control group of the respective analytes. Conclusion : Our results revealed that hyperthyroidism was associated with decrease total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and slightly increased triglycerides. LDL and negative correlation, but not significant between TSH a pond TGs. These findings are similar with the report of Shashi A and Sharma N [31]. They reported positive correlations between serum TSH and TC, TSH and LDL, TSH and HDL, but not with TSH and TG. On the other positive correlations changes in resting subsequent loss of disparities findings compared previous findings differences in the research design and the subjects used in the studies.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01Epub Date: 2017-02-21DOI: 10.16966/2572-9578.107
Sandra L Rebholz, John T Melchior, Jeffrey A Welge, Alan T Remaley, W Sean Davidson, Laura A Woollett
World-wide, millions of women enter preterm labor or have small newborns. Effective biomarkers are needed to identify women at risk for these adverse outcomes. A time and cost effective way to examine any potentially new biomarkers in samples collected during prior studies or trials that had been assayed for other metabolites would be highly useful. Thus, the current study aimed to determine if samples that had been previously thawed and re-frozen could be re-assayed for novel biomarkers, those being lipoprotein composition (sizing, proteome, lipids) and combined cholesterol and cytokine concentrations. Fasting blood was collected from 51 young non-pregnant women and plasma was analyzed for lipoprotein composition and cytokine concentrations after multiple freeze/thaw cycles in the cold or at room temperature and after being stored for 18 months. Plasma LDL-C, HDL-C, total cholesterol, and triglyceride concentrations decreased <6-7% (cholesterols) or <20% (triglyceride) after 7 thaws in the cold, 3 thaws at room temperature, and after 18 months of storage. As these decreases were less than day-to-day reported variation of lipids, they do not appear to be physiologically significant. Cytokine (IL-6, TNF α, IL-8, IL-1β) and hsCRP concentrations decreased by 22%, 8%, 8%, 22%, and 35%, respectively; only IL-6, IL-1β and hsCRP concentrations showed significant decreases greater than day-to-day variations of 20%. For measured triglyceride and cytokine, but not cholesterol concentrations, decreases with freeze/thaw cycles were greater when concentrations were elevated. Multiple thaws also led to changes in lipoprotein sizing, specifically to a shift from medium- and large-sized HDL particles to small-sized HDL particles and from large LDL to IDL. No changes occurred for VLDL particle numbers. Though particle sizes changed, the HDL proteome did not change with multiple thaw cycles or after long term storage. Overall, the results demonstrate that it is possible to use previously obtained frozen samples for plasma cholesterol and triglyceride levels and the lipoprotein proteome, and lipoprotein sizing and cytokine concentrations if one knows the history of the sample as changes should be relative to one another.
{"title":"Effects of Multiple Freeze/Thaw Cycles on Measurements of Potential Novel Biomarkers Associated With Adverse Pregnancy Outcomes.","authors":"Sandra L Rebholz, John T Melchior, Jeffrey A Welge, Alan T Remaley, W Sean Davidson, Laura A Woollett","doi":"10.16966/2572-9578.107","DOIUrl":"https://doi.org/10.16966/2572-9578.107","url":null,"abstract":"<p><p>World-wide, millions of women enter preterm labor or have small newborns. Effective biomarkers are needed to identify women at risk for these adverse outcomes. A time and cost effective way to examine any potentially new biomarkers in samples collected during prior studies or trials that had been assayed for other metabolites would be highly useful. Thus, the current study aimed to determine if samples that had been previously thawed and re-frozen could be re-assayed for novel biomarkers, those being lipoprotein composition (sizing, proteome, lipids) and combined cholesterol and cytokine concentrations. Fasting blood was collected from 51 young non-pregnant women and plasma was analyzed for lipoprotein composition and cytokine concentrations after multiple freeze/thaw cycles in the cold or at room temperature and after being stored for 18 months. Plasma LDL-C, HDL-C, total cholesterol, and triglyceride concentrations decreased <6-7% (cholesterols) or <20% (triglyceride) after 7 thaws in the cold, 3 thaws at room temperature, and after 18 months of storage. As these decreases were less than day-to-day reported variation of lipids, they do not appear to be physiologically significant. Cytokine (IL-6, TNF α, IL-8, IL-1β) and hsCRP concentrations decreased by 22%, 8%, 8%, 22%, and 35%, respectively; only IL-6, IL-1β and hsCRP concentrations showed significant decreases greater than day-to-day variations of 20%. For measured triglyceride and cytokine, but not cholesterol concentrations, decreases with freeze/thaw cycles were greater when concentrations were elevated. Multiple thaws also led to changes in lipoprotein sizing, specifically to a shift from medium- and large-sized HDL particles to small-sized HDL particles and from large LDL to IDL. No changes occurred for VLDL particle numbers. Though particle sizes changed, the HDL proteome did not change with multiple thaw cycles or after long term storage. Overall, the results demonstrate that it is possible to use previously obtained frozen samples for plasma cholesterol and triglyceride levels and the lipoprotein proteome, and lipoprotein sizing and cytokine concentrations if one knows the history of the sample as changes should be relative to one another.</p>","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35241782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: To test the hypothesis that cooperative interactions between transcription factors Nuclear Factor-kappa B (NF-κB) and Activator Protein-1 (AP1) augment transcriptional activity in exocrine pancreatic cells.
{"title":"Clinical and Laboratory Medicine-Sci Forschen Full Text RESEARCH ARTICLE Exocrine Pancreatic Cell NF-κB/AP-1 Crosstalk Downstream of ERK MAPK","authors":"Twait Ec, Samuel I","doi":"10.16966/2572-9578.137","DOIUrl":"https://doi.org/10.16966/2572-9578.137","url":null,"abstract":"Aim: To test the hypothesis that cooperative interactions between transcription factors Nuclear Factor-kappa B (NF-κB) and Activator Protein-1 (AP1) augment transcriptional activity in exocrine pancreatic cells.","PeriodicalId":92069,"journal":{"name":"Journal of clinical and laboratory medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67394740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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