Journal of the Marine Biological Association of India. Marine Biological Association of India最新文献

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Report of an amphipod, Caprella danilevskii (Czerniavskii,1868), from the south Saurashtra coast, Gujarat, India 印度古吉拉特邦南苏拉特拉海岸片脚类Caprella danilevskii (Czerniavskii,1868)报告

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2181-16

N. Gajera, S. Jethava, R. Kundu

引用次数: 0

Seasonal and spatial variability of zooplankton diversity in North Eastern Arabian Sea along the Maharashtra coast 马哈拉施特拉邦沿海阿拉伯海东北部浮游动物多样性的季节和空间变异

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2293-04

Punam A. Khandagale, V. D. Mhatre, Sujitha Thomas

The study focused on the variations in seasonal diversity of zooplankton between the polluted coastal environments of Mumbai and the comparatively less polluted coastal waters of Ratnagiri. The study was carried out from August 2014 to May 2017. Zooplankton was collected from hauls at 20 m and 40 m depth contours off Mumbai and Ratnagiri coast. The results indicated that Species Richness d, Shannon H and Simpson 1-λ at Mumbai stations were low compared to Ratnagiri stations. The values were higher in post-monsoon (POM) and pre-monsoon (PRM) than in monsoon (MON) season. Two-way ANOVA revealed a significant difference (P<0.05) for values of ‘d’, ‘H’ and 1-λ ’ between Mumbai and Ratnagiri. Post-Hoc Tukey test showed a significant difference (P<0.05) between values of ‘d’, ‘H’ and 1-λ ’ during MON and POM, MON and PRM. However, no significant difference (P>0.05) was observed between POM and PRM. Cluster analysis showed a 35% similarity in diversity between MON and the other two seasons. SIMPER analysis of similarity showed average dissimilarity of 44.77 between the diversity of Mumbai and Ratnagiri. The study indicated seasonal variations in the zooplankton diversity in coastal regions of Mumbai and Ratnagiri. The impact of pollution and anthropogenic activities could have reduced zooplankton diversity and abundance in the coastal environment of Mumbai compared to the less polluted waters of Ratnagiri.

研究的重点是受污染的孟买沿海环境和污染相对较少的Ratnagiri沿海水域之间浮游动物的季节性多样性变化。该研究于2014年8月至2017年5月进行。在孟买和拉特纳吉里海岸的20米和40米深度轮廓处收集浮游动物。结果表明，孟买站的物种丰富度d、Shannon H和Simpson 1-λ较Ratnagiri站低。季风后(POM)和季风前(PRM)的数值高于季风季节(MON)。双因素方差分析显示，POM与PRM之间存在显著差异(P0.05)。聚类分析表明，冬小麦与其他两个季节的多样性相似性为35%。相似度的SIMPER分析显示孟买和拉特纳吉里的多样性平均差异为44.77。该研究表明，孟买和拉特纳吉里沿海地区浮游动物多样性的季节性变化。与Ratnagiri污染较少的水域相比，污染和人为活动的影响可能减少了孟买沿海环境中浮游动物的多样性和丰度。

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引用次数: 1

Tracking the origin of polypropylene and polyethylene bags in coral reefs of the Gulf of Mannar 追查马纳尔湾珊瑚礁中聚丙烯和聚乙烯袋的来源

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2202-19

C. Ramesh, S. Koushik, T. Shunmugaraj, M. V. Ramana Murthy

引用次数: 0

Rediscovery of Ram’s horn squid, Spirula spirula (Cephalopoda: Spirulidae), from the Arabian Se 阿拉伯海公角鱿鱼，螺旋藻(头足纲:螺旋藻科)的再发现

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2282-12

K. K. Sajikumar, M. P. Rajeesh Kumar, P. Laxmilatha, M. Hashim, V. Venkatesan, N. Saravanane

This study describes the rediscovery of the largely uncommon, mesopelagic cephalopod, Spirula spirula, from the Arabian Sea. This is the first confirmed record of the species from the seas around India after 80 years of its original record. Four specimens were collected from the Arabian Sea from a depth of 450 m, during the fishery surveys onboard the FORV Sagar Sampada, using a mid-water trawl net. The dorsal mantle length (DML) of the individuals ranged from 27 to 36 mm. Detailed morphometric measurements and previous distribution records are also provided.

这项研究描述了从阿拉伯海重新发现的一种非常罕见的中远洋头足类动物螺旋藻。这是印度附近海域首次确认的该物种的记录，此前已有80年的记录。在FORV Sagar Sampada上进行渔业调查期间，使用中水拖网在阿拉伯海450米深处收集了四个标本。个体的背地幔长度(DML)在27 ~ 36 mm之间。还提供了详细的形态测量和以前的分布记录。

引用次数: 0

Population genetics of Indian white shrimp, Penaeus indicus (H. Milne Edwards, 1837) from Indian waters 印度水域印度白对虾(Penaeus indicus, H. Milne Edwards, 1837)的种群遗传学

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2295-01

J. R. Jani Angel, K. Vinaya, T. Vinay, M. S. Shekhar, A. Panigrahi, C. P. Balasubramanian, G. Gopikrishna, K. K. Vijayan

引用次数: 0

An account of the wild collection of polychaete (Annelida) from the intertidal zones of Mumbai, Maharashtra, India 印度马哈拉施特拉邦孟买潮间带多毛纲动物(环节动物)的野生收集记录

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2232-15

N. Pawar, U. Rane, S. Bhendekar, A. Chellappan

The study was planned to examine polychaete fishing activity along the coast of Mumbai city. Random sampling was performed for a period of six months and conducted interviews with bait diggers, traders and local fishers (n=30). Polychaete annelids were found to be the most extensively harvested taxa in the intertidal areas due to market demand from the aquaculture industry. The collected polychaetes were identified as Marphysa spp. During the survey, each pair (two partners) on a typical day is reported to collect around 1-2 kilogram of live worms, which earns them ` 1000 per kg. It is recorded that the average collection of worms from various locations in intertidal areas of Mumbai city is around 150-200 kg/day. Last decade, the annual consumption of polychaetes by shrimp hatcheries ranged from 16 to 20 tonnes. Shrimp farming in India is intensified and currently, there are 315 hatcheries registered with the Government of India for seed production of Litopenaeus vannamei. The demand for polychaete worms has therefore increased many folds and could be estimated in the range of 252 to 337 tonnes annually and nowadays, this demand is being met by an unreported wild-caught polychaete resource as well as import of Specific Pathogen Free (SPF) polychaete from overseas suppliers. There is scope for polychaete culture to satisfy the country’s growing market demand. Moreover, polychaete fishing in coastal areas removes substantial biomass and hence can cause major impacts on coastal habitats. There is a need for regulation of its mass collection from the wild.

该研究计划调查孟买市沿海的多毛类捕捞活动。随机抽样为期6个月，并对采饵者、贸易商和当地渔民进行访谈(n=30)。由于水产养殖业的市场需求，多毛纲环节动物被发现是潮间带最广泛收获的分类群。收集到的多毛动物被确定为Marphysa spp.在调查期间，每对(两个伴侣)在一个典型的一天中收集大约1-2公斤的活蠕虫，每公斤可以赚1000美元。据记录，从孟买市潮间带不同地点收集的蠕虫平均每天约150-200公斤。过去十年，虾类孵化场对多毛体的年消耗量在16至20吨之间。印度的对虾养殖得到加强，目前在印度政府登记了315个孵化场，用于生产凡纳滨对虾的种子。因此，对多毛类蠕虫的需求增加了许多倍，估计每年的需求量在252至337吨之间。如今，这一需求正在通过未报告的野生捕获多毛类资源以及从海外供应商进口的无特定病原体(SPF)多毛类来满足。多毛类文化有满足该国日益增长的市场需求的空间。此外，沿海地区的多毛类捕捞消除了大量生物量，因此可能对沿海生境造成重大影响。有必要对从野外采集的大量野生动物进行监管。

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引用次数: 0

Ultrastructure of distal vas deferens and terminal ampoule of the green tiger prawn Penaeus semisulcatus De Haan 1844 半虎对虾(Penaeus semiulcatus De Haan 1844)远端输精管和末端安瓿的超微结构

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2184-14

K. Sivachandrabose

引用次数: 0

Distribution and diversity of phytoplankton in Kadalundi estuary, southwest coast of India 印度西南海岸Kadalundi河口浮游植物的分布和多样性

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2279-08

M. Varghese, K. Vinod, R. Gireesh, A. Anasukoya, C. Ansar, M. Nikhiljith, K. Sheeba, P. K. Asokan, K. Joshi

引用次数: 1

Post capture survival rate of bamboo sharks, Chiloscyllium arabicum and Chiloscyllium griseum, in Malvan, Maharashtra 马哈拉施特拉邦马尔万地区阿拉伯竹鲨和灰竹鲨捕获后的存活率

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2022-03-30 DOI: 10.6024/jmbai.2022.64.1.2216-13

S. Kottillil, T. Gupta, M. Manoharakrishnan, C. Rao, K. Shanker

引用次数: 0

Isolation and identification of marine Bacillus altitudinis KB1 from coastal Kerala: asparaginase producer. 从喀拉拉邦沿海分离和鉴定海洋杆菌 KB1：天冬酰胺酶生产者。

Journal of the Marine Biological Association of India. Marine Biological Association of India

Pub Date : 2021-07-01 Epub Date: 2021-10-30 DOI: 10.6024/jmbai.2021.63.2.2195-06

Wael Ali Mohammed Hadi, Boby T Edwin, Ananthakrishnan Jayakumaran Nair

L-asparaginase is a target for many researchers as its properties against cancer, especially leukaemia, and protective agents reduce acrylamide in fried food. In this study, the water samples from Thumba Arattuvazhi Beach in Kerala were screened for l-asparaginase producing microorganisms. This was followed by colourimetric screening using modified M9 media with 0.009% Phenol red dye and using l-asparagine as a sole nitrogen source. Then, the Nessler assay was performed to quantify the enzyme. Molecular identification was made by 16SrRNA sequencing and aligned the sequence with GeneBank for phylogenetic tree construction using BLAST. Seawater was serially diluted for 10^-1 to 10^-6 using nutrient agar plates. A total of 19 bacterial colonies were isolated. The colonies were evaluated to produce l-asparaginase according to the pink zone around the colonies on the modified M9 medium using a red phenol indicator. The KB1 sample was selected for further studies according to plate colour assay. Nessler assay of L-asparaginase quantified as 2.537 IU/ml. Molecular characterisation showed the sequence association with Bacillus altitudinis the sequence submitted in Genebank as B. altitudinis KB1 strain. The l-asparaginase II gene (AnsB) was amplified based on the entire length of the hypothetical protein of annotated genome with accession number CP022319.2. The l-asparaginase activity in this study was 57% higher than the reference organism B. altitudinis BITHSP010. The l-asparaginase producing bacterium B. altitudinis KB1 from a marine source in Kerala can produce asparaginase, which can be utilised for biotechnology applications.

由于 L-天冬酰胺酶具有抗癌（尤其是白血病）和减少油炸食品中丙烯酰胺的保护作用，因此是许多研究人员的研究目标。在这项研究中，对喀拉拉邦 Thumba Arattuvazhi 海滩的水样进行了筛选，以检测是否有产生 L-天冬酰胺酶的微生物。随后，使用改良的 M9 培养基和 0.009% 的苯酚红染料进行比色筛选，并将 l-天冬酰胺作为唯一的氮源。然后，采用奈斯勒测定法对酶进行定量。通过 16SrRNA 测序进行分子鉴定，并将序列与 GeneBank 进行比对，使用 BLAST 构建系统发生树。使用营养琼脂平板对海水进行 10-1 至 10-6 的系列稀释。共分离出 19 个细菌菌落。使用红色酚指示剂在改良的 M9 培养基上根据菌落周围的粉红色区域来评估菌落是否产生 l-天冬酰胺酶。根据平板颜色检测结果，选择 KB1 样品进行进一步研究。奈斯勒法检测 L-天冬酰胺酶的定量为 2.537 IU/ml。分子鉴定结果表明，其序列与高度芽孢杆菌（Bacillus altitudinis）有关，该序列已作为高度芽孢杆菌 KB1 株提交至基因库。根据登录号为 CP022319.2 的基因组注释中假设蛋白的整个长度扩增了 l-天冬酰胺酶 II 基因（AnsB）。与参考生物 B. altitudinis BITHSP010 相比，本研究中的 l-天冬酰胺酶活性高出 57%。喀拉拉邦海洋来源的天冬酰胺酶产生菌 B. altitudinis KB1 能产生天冬酰胺酶，可用于生物技术应用。

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