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Maturation and development of fetal pig intestinal tissue in immunodeficient mice. 免疫缺陷小鼠胎猪肠道组织的成熟与发育
Pub Date : 2024-02-26 eCollection Date: 2024-01-01 DOI: 10.1590/acb390624
Atsushi Harada, Naoto Matsumoto, Yoshitaka Kinoshita, Kenji Matsu, Yuka Inage, Keita Morimoto, Shuichiro Yamanaka, Masashi Kurobe, Takashi Yokoo, Haruki Kume, Takao Ohki, Eiji Kobayashi

Purpose: This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice.

Methods: Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated.

Results: The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed.

Conclusions: Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.

目的:本研究旨在比较胎猪节段性肠道组织的成熟和发育程度,以及将离体的胎儿肠道细胞移植到免疫缺陷小鼠体内后在体外重新聚集形成的球形组织的成熟和发育程度:方法:将胎猪小肠分段移植到免疫缺陷小鼠的网膜和肾下囊,或经酶处理生成单细胞。将这些细胞在体外重新聚集而成的球状细胞移植到免疫缺陷小鼠的肾下囊。移植后 4 周和 8 周分别收获节段性移植物和球形体,并对这些标本的结构成熟度和体内发育情况进行组织学评估:结果:球体被移植并供应宿主小鼠的血管,但没有明显观察到肠道分层结构,大小也几乎没有变化。另一方面,节段性移植物在粘膜、内圆层和外纵肌中形成了深隐窝。与第四周收获的移植物相比,八周收获的移植物的隐窝更深,平滑肌层和肠神经系统更成熟,但未观察到肠蠕动波:结论:用胎儿小肠细胞制作的球形组织不能形成分层结构,也不能充分成熟。结论:用胎儿小肠细胞制作的球状体不能形成分层结构,也不能充分成熟,相反,节段组织在体内移植后结构成熟并发育,因此是潜在的移植组织。
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引用次数: 0
Clinical behavior of recurrent pleomorphic adenoma in the palate: a systematic review. 腭部复发性多形性腺瘤的临床表现:系统综述。
Pub Date : 2024-02-26 eCollection Date: 2024-01-01 DOI: 10.1590/acb390824
Gabriela Lopes-Santos, Najara Gomes de Oliveira Marques, Kellen Cristine Tjioe, Denise Tostes Oliveira

Purpose: This systematic review analyzed the clinical behavior and odds of malignancy of the palatal recurrent pleomorphic adenomas.

Methods: Systematic review of patients with recurrent pleomorphic adenoma arising in the palate. Database search: MEDLINE, Scopus, Web of Science, Cochrane, EMBASE, Virtual Health Library, Google Scholar, and OpenGrey. A binomial logistic regression was performed to assess the odds of detecting recurrence five, 10 and 20 years after the treatment of primary tumor.

Results: Thirteen studies (n = 18 patients) out of 336 were included. The recurrent pleomorphic adenoma in palate was more common in females (61.6%), average age was 49 years old (range 9-73 years old). Four patients progressed to malignant transformation. The odds ratio (OR) of detecting a recurrence at 10 (OR = 5.57; 95% confidence interval - 95%CI 1.13-27.52), and 20 years (OR = 18.78; 95%CI 3.18-110.84) after treatment of primary pleomorphic adenoma was significantly higher than at one-year follow-up.

Conclusions: The recurrence of pleomorphic adenoma in palate remains a rare event of late occurrence. It mainly affects middle-aged female and carries a risk of malignant transformation. Although uncommon, patients with palatal pleomorphic adenoma should be warned about the possibility of recurrence or malignant transformation of tumor at advanced ages.

目的:这篇系统综述分析了腭复发性多形性腺瘤的临床表现和恶变几率:方法:对腭部复发性多形性腺瘤患者进行系统回顾。数据库检索:MEDLINE、Scopus、Web of Science、Cochrane、EMBASE、Virtual Health Library、Google Scholar 和 OpenGrey。采用二项式逻辑回归评估原发肿瘤治疗后5年、10年和20年发现复发的几率:在 336 项研究中,有 13 项研究(n = 18 名患者)被纳入。腭部复发性多形性腺瘤多见于女性(61.6%),平均年龄为 49 岁(9-73 岁不等)。有 4 名患者发展为恶性肿瘤。原发性多形性腺瘤治疗后10年(OR=5.57;95%置信区间-95%CI 1.13-27.52)和20年(OR=18.78;95%CI 3.18-110.84)发现复发的几率(OR)明显高于一年随访时的几率:结论:腭部多形性腺瘤复发仍是一种罕见的晚期病变。结论:腭部多形性腺瘤复发仍是晚期发生的罕见病例,主要影响中年女性,并有恶变风险。腭多形性腺瘤虽然并不常见,但患者应警惕肿瘤晚期复发或恶变的可能性。
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引用次数: 0
Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway. 西诺明对碘乙酸钠诱导的大鼠膝关节和髋关节损伤的骨保护作用:炎症途径。
Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390924
Yi-Hao Lei, Xing-Xi Hu, Hong-Jie Wen, Yong-Cheng Deng, Jun-Liang Jiang, Qing-Gang Zhao

Purpose: Osteoarthritis (OA) is a degenerative joint disease which is categorized via destruction of joint cartilage and it also affects the various joints, especially knees and hips. Sinomenine active phytoconstituents isolated from the stem of Sinomenium acutum and already proof anti-inflammatory effect against the arthritis model of rodent. In this experimental protocol, we scrutinized the anti-osteoarthritis effect of sinomenine against monosodium iodoacetate (MIA) induced OA in rats.

Methods: MIA (3 mg/50 μL) was used for inducing the OA in the rats, and rats received the oral administration of sinomenine (2.5, 5 and 7.5 mg/kg body weight) up to the end of the experimental study (four weeks). The body and organs weight were estimated. Aggrecan, C-terminal cross-linked telopeptide of type II collagen (CTX-II), glycosaminoglycans (GCGs), monocyte chemoattractant protein-1 (MCP-1), Interferon gamma (IFN-γ), antioxidant, inflammatory cytokines, inflammatory mediators and matrix metalloproteinases (MMP) were analyzed.

Results: Sinomenine significantly (P < 0.001) boosted the body weight and reduced the heart weight, but the weight of spleen and kidney remain unchanged. Sinomenine significantly (P < 0.001) reduced the level of nitric oxide, MCP-1 and improved the level of aggrecan, IFN-γ and GCGs. Sinomenine remarkably upregulated the level of glutathione, superoxide dismutase and suppressed the level of malonaldehyde. It effectually modulated the level of inflammatory cytokines and inflammatory mediators and significantly (P < 0.001) reduced the level of MMPs, like MMP-1, 2, 3, 9 and 13.

Conclusions: Sinomenine is a beneficial active agent for the treatment of OA disease.

目的:骨关节炎(OA)是一种退行性关节疾病,主要表现为关节软骨的破坏,也会影响各个关节,尤其是膝关节和髋关节。从刺五加茎中分离出的刺五加碱是一种活性植物成分,已被证明对啮齿动物关节炎模型具有抗炎作用。在本实验方案中,我们研究了西诺明对碘乙酸钠(MIA)诱导的大鼠 OA 的抗骨关节炎作用:方法:用MIA(3 mg/50 μL)诱导大鼠OA,大鼠口服西诺明(2.5、5和7.5 mg/kg体重)至实验研究结束(四周)。对大鼠的体重和器官重量进行了估计。分析了凝集素、Ⅱ型胶原 C 端交联端肽(CTX-Ⅱ)、糖胺聚糖(GCGs)、单核细胞趋化蛋白-1(MCP-1)、γ 干扰素(IFN-γ)、抗氧化剂、炎症细胞因子、炎症介质和基质金属蛋白酶(MMP):西诺明能明显增加体重(P < 0.001),减轻心脏重量,但脾脏和肾脏的重量保持不变。西诺明能明显(P < 0.001)降低一氧化氮和 MCP-1 的水平,提高凝集素、IFN-γ 和 GCGs 的水平。西诺明能明显提高谷胱甘肽、超氧化物歧化酶的水平,抑制丙二醛的水平。它能有效调节炎症细胞因子和炎症介质的水平,并显著降低 MMPs(如 MMP-1、2、3、9 和 13)的水平(P < 0.001):结论:西诺明是一种有益于治疗 OA 疾病的活性制剂。
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引用次数: 0
Combined administration of gallic acid and glibenclamide mitigate systemic complication and histological changes in the cornea of diabetic rats induced with streptozotocin. 联合服用没食子酸和格列本脲可减轻全身并发症和链脲佐菌素诱导的糖尿病大鼠角膜的组织学变化。
Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390124
Jing Zhao, Shaik Althaf Hussain, Narendra Maddu

Purpose: To determine the effect of gallic acid or its combination with glibenclamide on some biochemical markers and histology of the cornea of streptozotocin (STZ) induced diabetic rats.

Methods: Following induction of diabetes, 24 male albino rats were divided into four groups of six rats each. Groups 1 and 2 (control and diabetic) received rat pellets and distilled water; group 3 (gallic acid) received rat pellets and gallic acid (10 mg/kg, orally) dissolved in the distilled water; and group 4 (gallic acid + glibenclamide) received rat pellets, gallic acid (10 mg/kg, orally), and glibenclamide (5 mg/kg, orally) dissolved in the distilled water. The treatments were administered for three months after which the rats were sacrificed after an overnight fast. Blood and sera were collected for the determination of biochemical parameters, while their eyes were excised for histology.

Results: STZ administration to the rats induced insulin resistance, hyperglycemia, microprotenuria, loss of weight, oxidative stress, inflammation, and alteration of their cornea histology, which was abolished following supplementation with gallic acid or its combination with glibenclamide.

Conclusions: The study showed the potentials of gallic acid and glibenclamide in mitigating systemic complication and histological changes in the cornea of diabetic rats induced with STZ.

目的:确定没食子酸或其与格列本脲联合使用对链脲佐菌素(STZ)诱导的糖尿病大鼠角膜的一些生化指标和组织学的影响:诱导糖尿病大鼠后,将 24 只雄性白化大鼠分为 4 组,每组 6 只。第 1 组和第 2 组(对照组和糖尿病组)接受大鼠颗粒和蒸馏水;第 3 组(没食子酸组)接受大鼠颗粒和溶于蒸馏水的没食子酸(10 毫克/千克,口服);第 4 组(没食子酸 + 格列本脲组)接受大鼠颗粒、没食子酸(10 毫克/千克,口服)和溶于蒸馏水的格列本脲(5 毫克/千克,口服)。治疗持续三个月后,大鼠在禁食一夜后被处死。收集血液和血清以测定生化指标,同时切除大鼠眼睛以进行组织学检查:结果:给大鼠服用 STZ 会诱发胰岛素抵抗、高血糖、微量蛋白尿、体重减轻、氧化应激、炎症和角膜组织学改变:研究表明,没食子酸和格列本脲具有减轻STZ诱导的糖尿病大鼠全身并发症和角膜组织学变化的潜力。
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引用次数: 0
Antibiotics prophylaxis at the time of catheter removal after radical prostatectomy: a systematic review of the literature and meta-analysis. 根治性前列腺切除术后拔除导管时的抗生素预防:文献系统回顾和荟萃分析。
Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390424
Letícia Lourenço Dos Santos, Isabela de Almeida Fraga, Vitor Amaral de Almeida, Andressa Hellen Ribeiro Santos, Isabelle Matos Almeida, Tatiana Roberta Nascimento, Breno Cordeiro Porto, Carlo Camargo Passerotti, Everson Luiz de Almeida Artifon, Jose Pinhata Otoch, José Arnaldo Shiomi da Cruz

Purpose: To conduct a systematic literature review with meta-analysis to identify whether antibiotic prophylaxis after removal of the indwelling urinary catheter reduces posterior infections.

Methods: A systematic literature review was conducted in the databases PubMed, Embase, Cochrane, Google Scholar, and Latin American and Caribbean Health Sciences Literature, using the keywords "antibiotics" AND "prostatectomy" AND "urinary catheter."

Results: Three articles were identified having the scope of our review, with 1,040 patients, which were subjected to our meta-analysis revealing a marginally significant decrease in the risk of urinary infection after indwelling urinary catheter removal (odds ratio-OR = 0.51; 95% confidence interval-95%CI 0.27-0.98; p = 0.04; I2 = 0%). No difference was found regarding the presence of bacteriuria (OR = 0.39; 95%CI 0.12-1.24; p = 0.11; I2 = 73%).

Conclusions: In our meta-analysis, there was a significant decrease in urinary tract infection with antibiotic prophylaxis after indwelling urinary catheter removal following radical prostatectomy.

目的:通过荟萃分析进行系统性文献综述,以确定拔除留置导尿管后使用抗生素预防是否会减少后发感染:使用关键词 "抗生素"、"前列腺切除术 "和 "导尿管",在PubMed、Embase、Cochrane、Google Scholar以及拉丁美洲和加勒比健康科学文献等数据库中进行了系统性文献综述:我们对三篇文章进行了荟萃分析,发现在拔除留置导尿管后,泌尿系统感染的风险略有显著降低(几率比-OR = 0.51;95%置信区间-95%CI 0.27-0.98;P = 0.04;I2 = 0%)。在是否存在菌尿方面没有发现差异(OR = 0.39;95% 置信区间-95%CI 0.12-1.24;P = 0.11;I2 = 73%):在我们的荟萃分析中,根治性前列腺切除术后拔除留置导尿管后使用抗生素预防尿路感染的情况明显减少。
{"title":"Antibiotics prophylaxis at the time of catheter removal after radical prostatectomy: a systematic review of the literature and meta-analysis.","authors":"Letícia Lourenço Dos Santos, Isabela de Almeida Fraga, Vitor Amaral de Almeida, Andressa Hellen Ribeiro Santos, Isabelle Matos Almeida, Tatiana Roberta Nascimento, Breno Cordeiro Porto, Carlo Camargo Passerotti, Everson Luiz de Almeida Artifon, Jose Pinhata Otoch, José Arnaldo Shiomi da Cruz","doi":"10.1590/acb390424","DOIUrl":"10.1590/acb390424","url":null,"abstract":"<p><strong>Purpose: </strong>To conduct a systematic literature review with meta-analysis to identify whether antibiotic prophylaxis after removal of the indwelling urinary catheter reduces posterior infections.</p><p><strong>Methods: </strong>A systematic literature review was conducted in the databases PubMed, Embase, Cochrane, Google Scholar, and Latin American and Caribbean Health Sciences Literature, using the keywords \"antibiotics\" AND \"prostatectomy\" AND \"urinary catheter.\"</p><p><strong>Results: </strong>Three articles were identified having the scope of our review, with 1,040 patients, which were subjected to our meta-analysis revealing a marginally significant decrease in the risk of urinary infection after indwelling urinary catheter removal (odds ratio-OR = 0.51; 95% confidence interval-95%CI 0.27-0.98; p = 0.04; I2 = 0%). No difference was found regarding the presence of bacteriuria (OR = 0.39; 95%CI 0.12-1.24; p = 0.11; I2 = 73%).</p><p><strong>Conclusions: </strong>In our meta-analysis, there was a significant decrease in urinary tract infection with antibiotic prophylaxis after indwelling urinary catheter removal following radical prostatectomy.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390424"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flap of the sternocephalicus muscle in the repair of a partial defect in the trachea of a rabbit (Oryctolagus cuniculus). 修复兔子气管部分缺损的胸锁乳突肌瓣。
Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390324
Gustavo Fernandes, Ariadne Rein, Gabriel Luiz Montanhim, Marcelo Carrijo da Costa, Marcella Dall'Agnol Leite, Nicolle Pereira Soares, Paola Castro Moraes

Purpose: The current study aimed at evaluating the repair of a partial defect of the trachea with a muscle flap, an advanced technique that employs combined suture patterns.

Methods: Sixteen healthy male New Zealand white rabbits were used as an experimental model. A partial defect in the trachea within the ventral region of the fourth to eighth tracheal ring was created. Subsequently, repair was initiated with a flap of the sternocephalicus muscle. The animals were divided into four groups for postoperative evaluation using clinical, tracheoscopic, and histopathological analyses. Each group was separated according to the time of euthanasia, programmed at interval of seven (G7), 15 (G15), 30 (G30), and 60 days (G60).

Results: One animal from the G60 group died, whereas the other animals had good surgical recovery without serious changes in the breathing pattern. The major clinical signs observed were stridor and coughing. Tracheoscopy revealed secretions in the tracheal lumen, exuberant granulation, and stenosis. Histopathological analysis showed growth of the ciliary respiratory epithelium at the flap site 30 days after implantation.

Conclusions: Partial repair showed satisfactory results owing to the anatomical location of the muscle, adequate vascular support, and structural and physiological maintenance without serious changes in the respiratory system.

目的:本研究旨在评估用肌肉瓣修复气管部分缺损的效果,这是一种采用联合缝合模式的先进技术:方法:以 16 只健康雄性新西兰白兔为实验模型。方法:以 16 只健康的雄性新西兰白兔为实验模型,在第四至第八气管环的腹侧区域创建部分气管缺损。随后,用胸骨肌皮瓣进行修复。动物被分为四组,通过临床、气管镜和组织病理学分析进行术后评估。每组根据安乐死的时间分为7天组(G7)、15天组(G15)、30天组(G30)和60天组(G60):G60 组有一只动物死亡,而其他动物手术后恢复良好,呼吸模式没有发生严重变化。观察到的主要临床症状是喘鸣和咳嗽。气管镜检查发现气管腔内有分泌物、大量肉芽和狭窄。组织病理学分析显示,植入皮瓣 30 天后,皮瓣部位的纤毛呼吸道上皮增生:由于肌肉的解剖位置、充分的血管支持、结构和生理上的维护,部分修复术取得了令人满意的效果,呼吸系统没有发生严重变化。
{"title":"Flap of the sternocephalicus muscle in the repair of a partial defect in the trachea of a rabbit (Oryctolagus cuniculus).","authors":"Gustavo Fernandes, Ariadne Rein, Gabriel Luiz Montanhim, Marcelo Carrijo da Costa, Marcella Dall'Agnol Leite, Nicolle Pereira Soares, Paola Castro Moraes","doi":"10.1590/acb390324","DOIUrl":"10.1590/acb390324","url":null,"abstract":"<p><strong>Purpose: </strong>The current study aimed at evaluating the repair of a partial defect of the trachea with a muscle flap, an advanced technique that employs combined suture patterns.</p><p><strong>Methods: </strong>Sixteen healthy male New Zealand white rabbits were used as an experimental model. A partial defect in the trachea within the ventral region of the fourth to eighth tracheal ring was created. Subsequently, repair was initiated with a flap of the sternocephalicus muscle. The animals were divided into four groups for postoperative evaluation using clinical, tracheoscopic, and histopathological analyses. Each group was separated according to the time of euthanasia, programmed at interval of seven (G7), 15 (G15), 30 (G30), and 60 days (G60).</p><p><strong>Results: </strong>One animal from the G60 group died, whereas the other animals had good surgical recovery without serious changes in the breathing pattern. The major clinical signs observed were stridor and coughing. Tracheoscopy revealed secretions in the tracheal lumen, exuberant granulation, and stenosis. Histopathological analysis showed growth of the ciliary respiratory epithelium at the flap site 30 days after implantation.</p><p><strong>Conclusions: </strong>Partial repair showed satisfactory results owing to the anatomical location of the muscle, adequate vascular support, and structural and physiological maintenance without serious changes in the respiratory system.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390324"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10852536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of rosmarinic acid and doxorubicine on an ovarian adenocarsinoma cell line (OVCAR3) via the EGFR pathway. 迷迭香酸和多柔比星通过表皮生长因子受体途径对卵巢腺癌细胞系(OVCAR3)的影响
Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390524
Umut Sarı, Fuat Zaman

Purpose: We aimed to reveal the effects of rosmarinic acid (RA), which has come to the forefront with its antitumor and antioxidant properties in many studies recently in the ovarian adenocarcinoma cell line, on the epidermal growth factor receptor (EFGR) signaling pathway in the presence of doxorubicin (DOX).

Methods: Ovarian adenocarcinoma cell line (OVCAR3) and human skin keratinocyte cell line human skin keratinocyte cell line (HaCaT) were used as control. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was applied to determine the effect of RA and DOX on the proliferation of OVCAR3 and HaCaT cells. Bcl2 expression and epidermal growth factor receptor (EGFR) and western blot analysis were performed to determine the expression levels of the markers.

Results: It was determined that RA (IC50 = 437.6 μM) and DOX (IC50 = 0.08 μM) have the ability to inhibit the proliferation of OVCAR3 cells and induce apoptosis in a 72-hour time and dose-dependent manner. Western blot showed that the expression level of Bcl-2 and EGFR in OVCAR3 cells was down-regulated by RA and DOX.

Conclusions: Apoptosis in OVCAR3 cells can potentially be induced by RA via the EGFR pathway, and RA may be a potent agent for cancer therapy.

目的:我们旨在揭示迷迭香酸(RA)在多柔比星(DOX)作用下对表皮生长因子受体(EFGR)信号通路的影响:卵巢腺癌细胞株(OVCAR3)和人类皮肤角质细胞株人类皮肤角质细胞株(HaCaT)作为对照。(采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)检测 RA 和 DOX 对 OVCAR3 和 HaCaT 细胞增殖的影响。通过 Bcl2 表达和表皮生长因子受体(EGFR)及 Western 印迹分析来确定标记物的表达水平:结果表明,RA(IC50 = 437.6 μM)和DOX(IC50 = 0.08 μM)能够抑制OVCAR3细胞的增殖,并在72小时内以时间和剂量依赖的方式诱导细胞凋亡。Western印迹显示,RA和DOX可下调OVCAR3细胞中Bcl-2和表皮生长因子受体(EGFR)的表达水平:结论:RA可通过表皮生长因子受体途径诱导OVCAR3细胞凋亡,RA可能是一种有效的癌症治疗药物。
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引用次数: 0
Effects of chronic restraint stress in the prostate of prepubertal and adult rats. 慢性抑制性应激对青春期前和成年大鼠前列腺的影响。
Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb387123
Isabella Mendes Procópio, Carina Teixeira Ribeiro, Roger Gaspar Marchon, Waldemar Silva Costa, Gabriela Faria Buys-Gonçalves, Francisco José Barcellos Sampaio, Marco Aurélio Pereira-Sampaio, Diogo Benchimol de Souza

Purpose: To investigate the effects of chronic stress in the prostate of prepubertal and adult rats.

Methods: Thirty-two male rats were assigned into four groups depending on the type of treatment (control or stressed) and the age at which stress was initiated (prepubertal or adult). Restraint stress stimuli were applied for six weeks. Stressed prepubertal and adult rats evaluated immediately after the last stress stimuli were named SP and SA groups, respectively. Age-matched rats were used as control groups (CP and CA). At the end of the experiment, the rats were euthanized, and prostate morphological parameters were evaluated and statistically compared.

Results: Application of stress stimuli to the SP group resulted in reduced body weight, but no prostate morphological modification was noted. The SA group showed reduced testosterone level and prostatic epithelium surface density, in comparison to CA group. Further, the prostatic lumen surface density was increased in adult stressed animals, in comparison to adult controls.

Conclusions: The stress stimuli promoted changes in hormonal and morphological parameters in the prostate of adult stressed rats. Prepubertal stressed animals did not presented modifications of prostate morphology.

目的:探讨慢性应激对青春期前和成年大鼠前列腺的影响。方法:32只雄性大鼠根据治疗类型(对照组或应激组)和应激起始年龄(青春期前或成年期)分为4组。约束应激刺激持续6周。最后一次应激刺激后立即评估的青春期前应激大鼠和成年大鼠分别命名为SP组和SA组。取年龄匹配的大鼠作为对照组(CP和CA)。实验结束后对大鼠实施安乐死,评估前列腺形态学参数并进行统计学比较。结果:应激刺激SP组大鼠体重减轻,但前列腺形态未见改变。与CA组相比,SA组睾酮水平降低,前列腺上皮表面密度降低。此外,与成年对照组相比,成年应激动物的前列腺腔表面密度增加。结论:应激刺激可促进成年应激大鼠前列腺激素及形态参数的变化。青春期前应激动物没有出现前列腺形态的改变。
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引用次数: 0
Are both distinct epithelial and stromal cells molecular analysis from phyllodes tumors versus fibroadenoma components affected in breast fibroepithelial progression? 叶状瘤和纤维腺瘤成分的不同上皮细胞和基质细胞分子分析是否影响乳腺纤维上皮的进展?
Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb386823
Ângela Flavia Logullo Waitzberg, Elisa Napolitano E Ferreira, Mabel Pinilla, Paulo Pineda, Andréa Cristina de Moraes Malinverni, Fernando Augusto Soares, Dirce Maria Carraro

Purpose: To determine molecular events involved in the tumorigenesis of phyllodes tumors (PT) and the role of each stromal (SC) and epithelial (EC) cell.

Methods: Frozen breast samples enriched with epithelial and stromal cells from three fibroadenomas and 14 PT were retrieved and laser microdissected. Sanger and polymerase chain reaction-based sequencing of exon 2 MED12 and TERT promoter hotspot mutations were performed; 44K microarray platform was used to analyze gene expression.

Results: All three fibroadenomas (FAs) presented mutations in MED12, but not in TERT, whose mutation was observed in five of the 14 PTs. EC and SC of each affected tumor displayed identical alterations. Of the total differentially expressed genes (DEG) (EC = 1,543 and SC = 850), 984 were EC-eDEGs and 291 were SC-eDEGs. We found a high similarity of diseases and functions enriched by both cell types, but dissimilarity in the number of enriched canonical pathways. Three signaling canonical pathways overlapping with EC and SC were predicted to be activated in one cell type and inactivated in the other, while no overlap in eDEGs was assigned to them. We also identified 13 EC-eDEGs and five SC-eDEGs enriched networks, in which the SC-eDEGs were able to segregate FA from PT samples.

Conclusions: Identical TERT mutations from both SC and ES origins might affect the PTs tumorigenesis. Gene expression differences suggest coordinated molecular processes between these components with determinant differences acquired by SC, able to fully distinguish PTs from FAs lesions.

目的:探讨叶状瘤(PT)发生过程中涉及的分子事件以及各间质细胞(SC)和上皮细胞(EC)的作用。方法:从3例纤维腺瘤和14例纤维腺瘤中提取富含上皮细胞和基质细胞的冷冻乳腺标本,进行激光显微解剖。基于Sanger和聚合酶链反应对外显子2 MED12和TERT启动子热点突变进行测序;采用44K芯片平台分析基因表达。结果:所有3例纤维腺瘤(FAs)均在MED12中出现突变,但在TERT中没有,在14例PTs中有5例观察到其突变。每个受累肿瘤的EC和SC显示相同的改变。在总差异表达基因(DEG)中(EC = 1543, SC = 850), EC- edegs为984,SC- edegs为291。我们发现两种细胞类型富集的疾病和功能高度相似,但富集的典型途径数量不同。与EC和SC重叠的三个信号通路在一种细胞类型中被激活,而在另一种细胞类型中被灭活,而在edeg中没有重叠。我们还鉴定了13个EC-eDEGs和5个SC-eDEGs富集网络,其中SC-eDEGs能够从PT样品中分离FA。结论:SC和ES来源的相同TERT突变可能影响PTs的肿瘤发生。基因表达差异表明这些成分之间协调的分子过程与SC获得的决定差异,能够完全区分PTs和FAs病变。
{"title":"Are both distinct epithelial and stromal cells molecular analysis from phyllodes tumors versus fibroadenoma components affected in breast fibroepithelial progression?","authors":"Ângela Flavia Logullo Waitzberg, Elisa Napolitano E Ferreira, Mabel Pinilla, Paulo Pineda, Andréa Cristina de Moraes Malinverni, Fernando Augusto Soares, Dirce Maria Carraro","doi":"10.1590/acb386823","DOIUrl":"10.1590/acb386823","url":null,"abstract":"<p><strong>Purpose: </strong>To determine molecular events involved in the tumorigenesis of phyllodes tumors (PT) and the role of each stromal (SC) and epithelial (EC) cell.</p><p><strong>Methods: </strong>Frozen breast samples enriched with epithelial and stromal cells from three fibroadenomas and 14 PT were retrieved and laser microdissected. Sanger and polymerase chain reaction-based sequencing of exon 2 MED12 and TERT promoter hotspot mutations were performed; 44K microarray platform was used to analyze gene expression.</p><p><strong>Results: </strong>All three fibroadenomas (FAs) presented mutations in MED12, but not in TERT, whose mutation was observed in five of the 14 PTs. EC and SC of each affected tumor displayed identical alterations. Of the total differentially expressed genes (DEG) (EC = 1,543 and SC = 850), 984 were EC-eDEGs and 291 were SC-eDEGs. We found a high similarity of diseases and functions enriched by both cell types, but dissimilarity in the number of enriched canonical pathways. Three signaling canonical pathways overlapping with EC and SC were predicted to be activated in one cell type and inactivated in the other, while no overlap in eDEGs was assigned to them. We also identified 13 EC-eDEGs and five SC-eDEGs enriched networks, in which the SC-eDEGs were able to segregate FA from PT samples.</p><p><strong>Conclusions: </strong>Identical TERT mutations from both SC and ES origins might affect the PTs tumorigenesis. Gene expression differences suggest coordinated molecular processes between these components with determinant differences acquired by SC, able to fully distinguish PTs from FAs lesions.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparing financing models for supplementary healthcare in appendectomy: activity-based costing (fee-for-service) vs. diagnosis related group remuneration (bundled payment) - a systematic review and meta-analysis. 比较阑尾切除术补充医疗的融资模式:基于活动的成本核算(按服务收费)与诊断相关的团体报酬(捆绑支付)——一项系统回顾和荟萃分析。
Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb386923
André de Arimatéia de Souza Lino, Jose Arnaldo Shiomi da Cruz, Breno Cordeiro Porto, Rhuan Pimentel Nogueira, José Pinhata Otoch, Everson Luiz de Almeida Artifon

Purpose: In Brazil, healthcare services traditionally follow a fee-for-service (FFS) payment system, in which each medical procedure incurs a separate charge. An alternative reimbursement with the aim of reducing costs is diagnosis related group (DRG) remuneration, in which all patient care is covered by a fixed amount. This work aimed to perform a systematic review followed by meta-analysis to assess the effectiveness of the Budled Payment for Care Improvement (BPCI) versus FFS.

Methods: Our work was performed following the items of the PRISMA report. We included only observational trials, and the primary outcome assessed was the effectiveness of FFS and DRG in appendectomy considering complications. We also assessed the costs and length of hospital stay. Meta-analysis was performed with Rev Man version 5.4.

Results: Out of 735 initially identified articles, six met the eligibility criteria. We demonstrated a shorter hospital stay associated with the DRG model (mean difference = 0.39; 95% confidence interval - 95%CI - 0.38-0.40; p < 0.00001; I2 = 0%), however the hospital readmission rate was higher in this model (odds ratio = 1.57; 95%CI 1.02-2.44, p = 0.04; I2 = 90%).

Conclusions: This study reveals a potential decrease in the length of stay for appendectomy patients using the DRG approach. However, no significant differences were observed in other outcomes analysis between the two approaches.

目的:在巴西,医疗保健服务传统上遵循按服务收费(FFS)支付制度,其中每个医疗程序都需要单独收费。以降低费用为目的的另一种补偿办法是与诊断有关的小组(DRG)报酬,其中所有病人的护理都由固定数额支付。本研究旨在进行系统回顾,然后进行荟萃分析,以评估护理改善预算支付(BPCI)与FFS的有效性。方法:我们的工作按照PRISMA报告的项目进行。我们只纳入观察性试验,评估的主要结局是考虑并发症的FFS和DRG在阑尾切除术中的有效性。我们还评估了费用和住院时间。meta分析采用Rev Man 5.4版本。结果:在最初确定的735篇文章中,有6篇符合入选标准。我们证明了较短的住院时间与DRG模型相关(平均差异= 0.39;95%置信区间- 95% ci - 0.38-0.40;P < 0.00001;I2 = 0%),但该模型的再入院率较高(优势比= 1.57;95%CI 1.02 ~ 2.44, p = 0.04;I2 = 90%)。结论:本研究揭示了使用DRG入路的阑尾切除术患者住院时间的潜在减少。然而,两种方法在其他结果分析中没有观察到显著差异。
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Acta cirurgica brasileira
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