Cardiovascular Pathology最新文献

英文中文

Variable regional histomorphology of the ascending aorta: Implications for disease classification 升主动脉的可变区域组织形态：疾病分类的意义

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-10-08 DOI: 10.1016/j.carpath.2025.107787

Collin S. Pryma , Md. Shahrier Amin , Charles Leduc , Cecilia Wu , Sarah M. Jenkins , Melanie C. Bois , Joseph J. Maleszewski

Regional differences in the normal histology of the ascending aorta have not been systematically studied, possibly resulting in diagnostic misinterpretation. This study aims to characterize the normal histologic spectrum of the aortic sinus (AS) and tubular aorta (TA) in a normal population. Ascending aortic specimens from 37 autopsy cases (mean age 58.7 years, range 12 to >89 years) without known aortopathy were collected prospectively. Transverse and longitudinal sections from AS and TA were stained with H&E and Verhoeff–Van Gieson. Assessed features included medial and lamellar thickness, lamellar architecture, elastic lamina number, elastic fiber waviness and organization, and features of medial degeneration. Interobserver agreement was assessed using Krippendorff’s alpha. The AS had significantly thinner media and lamellae, more woven lamellar architecture, and greater elastic fiber waviness and non-parallel organization compared to the TA. Elastic lamina number was greater in the posterior TA than AS (p < 0.0001). Pathologist agreement was moderate to satisfactory except for lamellar architecture in the left and posterior AS. No significant differences were observed between the measures with age, sex, or body mass index up to 49 kg/m². These results provide the first systematic histologic profile of the AS and highlight key differences from the TA—several of which overlap with degenerative changes. Recognizing these normal regional variations is essential to avoid diagnostic overcalls and unnecessary intervention. Accurate specimen labeling, orientation, and sampling are critical in aortic pathology assessment.

升主动脉正常组织学的区域差异尚未系统研究，可能导致诊断误解。本研究的目的是表征正常人群的主动脉窦（AS）和管状主动脉（TA）的正常组织学谱。前瞻性地收集了37例无已知主动脉病变的尸检病例的升主动脉标本（平均年龄58.7岁，范围12 ~ 89岁）。AS和TA的横切面和纵切面用H&；E和verhoefff - van Gieson染色。评估特征包括内侧和板层厚度、板层结构、弹性板层数量、弹性纤维波纹度和组织以及内侧退变特征。使用Krippendorff的alpha来评估观察者间的一致性。与TA相比，AS具有更薄的介质和片层，更多的编织片层结构，更大的弹性纤维波浪形和非平行组织。后TA弹性椎板数目多于AS （p < 0.0001）。除了左侧和后部AS的板层结构外，病理一致性中等至令人满意。在年龄、性别或体重指数高达49 kg/m2的测量中，没有观察到显著差异。这些结果提供了AS的第一个系统组织学概况，并突出了与ta的关键差异，其中一些与退行性变化重叠。认识到这些正常的区域差异对于避免诊断过度和不必要的干预至关重要。准确的标本标记、定位和取样对主动脉病理评估至关重要。

{"title":"Variable regional histomorphology of the ascending aorta: Implications for disease classification","authors":"Collin S. Pryma , Md. Shahrier Amin , Charles Leduc , Cecilia Wu , Sarah M. Jenkins , Melanie C. Bois , Joseph J. Maleszewski","doi":"10.1016/j.carpath.2025.107787","DOIUrl":"10.1016/j.carpath.2025.107787","url":null,"abstract":"<div><div>Regional differences in the normal histology of the ascending aorta have not been systematically studied, possibly resulting in diagnostic misinterpretation. This study aims to characterize the normal histologic spectrum of the aortic sinus (AS) and tubular aorta (TA) in a normal population. Ascending aortic specimens from 37 autopsy cases (mean age 58.7 years, range 12 to >89 years) without known aortopathy were collected prospectively. Transverse and longitudinal sections from AS and TA were stained with H&E and Verhoeff–Van Gieson. Assessed features included medial and lamellar thickness, lamellar architecture, elastic lamina number, elastic fiber waviness and organization, and features of medial degeneration. Interobserver agreement was assessed using Krippendorff’s alpha. The AS had significantly thinner media and lamellae, more woven lamellar architecture, and greater elastic fiber waviness and non-parallel organization compared to the TA. Elastic lamina number was greater in the posterior TA than AS (p < 0.0001). Pathologist agreement was moderate to satisfactory except for lamellar architecture in the left and posterior AS. No significant differences were observed between the measures with age, sex, or body mass index up to 49 kg/m<sup>2</sup>. These results provide the first systematic histologic profile of the AS and highlight key differences from the TA—several of which overlap with degenerative changes. Recognizing these normal regional variations is essential to avoid diagnostic overcalls and unnecessary intervention. Accurate specimen labeling, orientation, and sampling are critical in aortic pathology assessment.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107787"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVER 4: Table of Contents/Barcode PMS 200 封面4：目录/条形码PMS 200

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-11-21 DOI: 10.1016/S1054-8807(25)00080-8

引用次数: 0

Clinical significance of C4d positivity within the first month after heart transplantation in detecting antibody-mediated rejection on endomyocardial biopsies 心脏移植术后1个月内C4d阳性检测心内膜活检抗体介导排斥反应的临床意义。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-09-20 DOI: 10.1016/j.carpath.2025.107783

Mengxue Zhang , Yan Zhou , Chrystalle Katte Carreon , Ann Nguyen , Tiana Riley , Aliya N. Husain , Huihua Li

Background

The pathologic definition for antibody-mediated rejection (AMR) includes both histopathological and immunopathological components. C4d is the most validated diagnostic marker for immunopathologic AMR; however, the clinical significance of early C4d positivity (≤1 month post-transplant) on endomyocardial biopsies (EMBs) is unknown.

Methods

Patients who had ≥1 episode of C4d-positive EMB within the first month after heart transplantation were selected, the coexistence with acute cellular rejection (ACR) and the correlations of C4d positivity with histopathologic features of AMR, clinical graft dysfunction, presence of donor specific antibodies (DSAs), and clinical outcomes were examined.

Results

112 EMBs from 46 patients were qualified and included in the study. 19 patients had single C4d-positive EMB whereas 27 patients developed multiple (2-4) episodes of C4d positivity within the first month. 40 % of C4d-positive EMBs showed concurrent ACR (26 with G1R, 6 with G2R). The C4d positivity correlated well with the histopathologic AMR, with 73 % of C4d-positive EMBs showing all or partial histologic features of AMR. Only 29 % of the C4d-positive EMBs were associated with clinical graft dysfunction, indicating that most early C4d-positive EMBs were clinically asymptomatic. DSAs were found positive in 28 patients (61 %), with preformed DSAs being more common than de novo DSAs. Although no cardiac allograft vasculopathy was observed in any patient, two pediatric patients died of AMR shortly after transplantation whereas three adult patients passed away mostly because of infection.

Conclusion

Heart transplant recipients with C4d-positive EMBs within the first month post-transplant were mainly asymptomatic; combined evaluation including clinical, pathological, and serological testing should be conducted for the best management of AMR.

背景：抗体介导的排斥反应（AMR）的病理定义包括组织病理学和免疫病理学两部分。C4d是最有效的免疫病理AMR诊断标志物；然而，早期C4d阳性（移植后≤1个月）在心内膜肌活检（EMBs）中的临床意义尚不清楚。方法：选取心脏移植术后1个月内C4d阳性EMB发作≥1次的患者，观察其是否伴有急性细胞排斥反应（ACR）， C4d阳性与AMR的组织病理学特征、临床移植物功能障碍、供体特异性抗体（dsa）的存在及临床结局的相关性。结果：46例患者的112例EMBs合格并纳入研究。19例患者为单一C4d阳性EMB，而27例患者在第一个月内出现多次（2-4次）C4d阳性发作。40%的c4d阳性EMBs并发ACR（26例G1R， 6例G2R）。C4d阳性与组织病理AMR相关，73%的C4d阳性EMBs表现出AMR的全部或部分组织学特征。只有29%的c4d阳性EMBs与临床移植物功能障碍相关，这表明大多数早期c4d阳性EMBs临床无症状。28例患者（61%）发现dsa阳性，预形成的dsa比新生dsa更常见。虽然没有观察到任何患者出现同种异体心脏移植血管病变，但有2例儿童患者在移植后不久死于AMR，而3例成人患者主要死于感染。结论：移植后1个月内c4d阳性EMBs心脏受者以临床无症状为主，应综合考虑临床、病理、血清学评价，以最佳处理AMR。

{"title":"Clinical significance of C4d positivity within the first month after heart transplantation in detecting antibody-mediated rejection on endomyocardial biopsies","authors":"Mengxue Zhang , Yan Zhou , Chrystalle Katte Carreon , Ann Nguyen , Tiana Riley , Aliya N. Husain , Huihua Li","doi":"10.1016/j.carpath.2025.107783","DOIUrl":"10.1016/j.carpath.2025.107783","url":null,"abstract":"<div><h3>Background</h3><div>The pathologic definition for antibody-mediated rejection (AMR) includes both histopathological and immunopathological components. C4d is the most validated diagnostic marker for immunopathologic AMR; however, the clinical significance of early C4d positivity (≤1 month post-transplant) on endomyocardial biopsies (EMBs) is unknown.</div></div><div><h3>Methods</h3><div>Patients who had ≥1 episode of C4d-positive EMB within the first month after heart transplantation were selected, the coexistence with acute cellular rejection (ACR) and the correlations of C4d positivity with histopathologic features of AMR, clinical graft dysfunction, presence of donor specific antibodies (DSAs), and clinical outcomes were examined.</div></div><div><h3>Results</h3><div>112 EMBs from 46 patients were qualified and included in the study. 19 patients had single C4d-positive EMB whereas 27 patients developed multiple (2-4) episodes of C4d positivity within the first month. 40 % of C4d-positive EMBs showed concurrent ACR (26 with G1R, 6 with G2R). The C4d positivity correlated well with the histopathologic AMR, with 73 % of C4d-positive EMBs showing all or partial histologic features of AMR. Only 29 % of the C4d-positive EMBs were associated with clinical graft dysfunction, indicating that most early C4d-positive EMBs were clinically asymptomatic. DSAs were found positive in 28 patients (61 %), with preformed DSAs being more common than de novo DSAs. Although no cardiac allograft vasculopathy was observed in any patient, two pediatric patients died of AMR shortly after transplantation whereas three adult patients passed away mostly because of infection.</div></div><div><h3>Conclusion</h3><div>Heart transplant recipients with C4d-positive EMBs within the first month post-transplant were mainly asymptomatic; combined evaluation including clinical, pathological, and serological testing should be conducted for the best management of AMR.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107783"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dilemma of endomyocardial biopsy sampling for lymphocytic myocarditis diagnosis 淋巴细胞性心肌炎诊断中心肌内膜活检取样的困境。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-09-04 DOI: 10.1016/j.carpath.2025.107778

Tatsuya Shiraki, Rika Kawakami, Renu Virmani

引用次数: 0

COVER 3: Editorial Board 封面3：编辑委员会

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-11-21 DOI: 10.1016/S1054-8807(25)00079-1

引用次数: 0

Pathological capillary analysis of ischemia with non-obstructive coronary arteries in a Fabry disease patient receiving enzyme replacement therapy 接受酶替代治疗的法布里病非阻塞性冠状动脉缺血的病理毛细血管分析。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-10-09 DOI: 10.1016/j.carpath.2025.107786

Hiromitsu Kanamori, Genki Naruse, Shingo Minatoguchi, Maya Ishiguro, Akihiro Yoshida, Hiroyuki Okura

Fabry disease is a lysosomal disorder caused by an α-galactosidase A deficiency, which often causes angina in the absence of epicardial coronary artery stenosis. It is conceivable that epicardial and microvascular coronary dysfunction due to globotriaosylceramide and related glycoshingolipid accumulation within the vasculature causes ischemia with non-obstructive coronary arteries (INOCA). However, the precise histology remained unknown. We present the case of a 44-year-old male diagnosed with Fabry disease and treated with enzyme replacement therapy (ERT), who was referred to our cardiology department after complaining of exertional and rest precordial discomfort. Electrocardiography showed left ventricular hypertrophy (LVH) with strained ST segment depression. Echocardiography showed normal wall motion with diffuse LVH. Although coronary angiography showed no organic stenosis, spasms were provoked by intracoronary ergonovine injection. Invasive assessment of the microvasculature physiology showed the mean index of microcirculatory resistance to be 42 and the mean coronary flow reserve to be 1.6, which is indicative of coronary microvascular dysfunction. Endomyocardial biopsy revealed abundant accumulation of lamellar bodies within both cardiomyocytes and capillary pericytes, but not endothelial cells, suggesting microvascular flow disruption attributable to pericyte contraction and capillary constriction. This case highlights INOCA associated with a microvascular lesion related to Fabry disease in a patient receiving ERT as well as the need to develop therapies targeting the capillary circulation.

法布里病是一种由半乳糖苷酶a缺乏引起的溶酶体疾病，在没有心外膜冠状动脉狭窄的情况下常引起心绞痛。可以想象，心外膜和微血管冠状动脉功能障碍是由于血管内的球三神经酰胺和相关的糖鞘脂积累引起的非阻塞性冠状动脉缺血（INOCA）。然而，精确的组织学仍然未知。我们提出一个44岁的男性诊断为法布里病和治疗的酶替代疗法（ERT），谁被转介到我们的心脏科后，抱怨劳累和休息心前不适。心电图显示左室肥厚伴ST段压迫。超声心动图显示壁运动正常，伴有弥漫性LVH。尽管冠状动脉造影未显示器质性狭窄，但冠状动脉内注射麦角碱引起痉挛。微血管生理有创评估显示，微循环阻力平均指数为42，平均冠状动脉血流储备为1.6，提示冠状动脉微血管功能障碍。心肌内膜活检显示心肌细胞和毛细血管周细胞内均有丰富的板层体积聚，但内皮细胞内没有，提示微血管流动中断可归因于周细胞收缩和毛细血管收缩。本病例强调了接受ERT的患者中与法布里病相关的微血管病变相关的INOCA，以及开发针对毛细血管循环的治疗的必要性。

{"title":"Pathological capillary analysis of ischemia with non-obstructive coronary arteries in a Fabry disease patient receiving enzyme replacement therapy","authors":"Hiromitsu Kanamori, Genki Naruse, Shingo Minatoguchi, Maya Ishiguro, Akihiro Yoshida, Hiroyuki Okura","doi":"10.1016/j.carpath.2025.107786","DOIUrl":"10.1016/j.carpath.2025.107786","url":null,"abstract":"<div><div>Fabry disease is a lysosomal disorder caused by an α-galactosidase A deficiency, which often causes angina in the absence of epicardial coronary artery stenosis. It is conceivable that epicardial and microvascular coronary dysfunction due to globotriaosylceramide and related glycoshingolipid accumulation within the vasculature causes ischemia with non-obstructive coronary arteries (INOCA). However, the precise histology remained unknown. We present the case of a 44-year-old male diagnosed with Fabry disease and treated with enzyme replacement therapy (ERT), who was referred to our cardiology department after complaining of exertional and rest precordial discomfort. Electrocardiography showed left ventricular hypertrophy (LVH) with strained ST segment depression. Echocardiography showed normal wall motion with diffuse LVH. Although coronary angiography showed no organic stenosis, spasms were provoked by intracoronary ergonovine injection. Invasive assessment of the microvasculature physiology showed the mean index of microcirculatory resistance to be 42 and the mean coronary flow reserve to be 1.6, which is indicative of coronary microvascular dysfunction. Endomyocardial biopsy revealed abundant accumulation of lamellar bodies within both cardiomyocytes and capillary pericytes, but not endothelial cells, suggesting microvascular flow disruption attributable to pericyte contraction and capillary constriction. This case highlights INOCA associated with a microvascular lesion related to Fabry disease in a patient receiving ERT as well as the need to develop therapies targeting the capillary circulation.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107786"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aortic valve morphology rather than aortic valve function, aortic dilatation, and age interferes with ascending aortic structural and biomechanical properties 主动脉瓣形态而不是主动脉瓣功能、主动脉扩张和年龄影响升主动脉结构和生物力学特性。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-09-18 DOI: 10.1016/j.carpath.2025.107782

Jan M. Federspiel , Jan-Christian Reil , Peter H. Schmidt , Paul Teping , Frank Ramsthaler , Tanja Schwab , Hans-Joachim Schäfers

Aortic valve (AV) malformation and AV malfunction have been linked to aortic wall degeneration. Studies concomitantly assessing AV morphology, AV function, age, ascending aortic dilatation, and aortic biomechanical properties are lacking. This exploratory study aims to close this gap. Surgical samples of the ascending aorta (n=102) were histologically assessed. Based on echocardiographic studies, the elastic modulus (slope stress-strain curve) was calculated. Patient characteristics were collected from the patient charts. Samples obtained during autopsy (n=10) served as reference for the microscopic analysis. The patient characteristics, structural aortic wall changes, and biomechanical wall properties were statistically explored using comparative analyses and a Spearman correlation matrix. Marked medial degeneration was found significantly earlier in life for unicuspid AV morphology compared to bicuspid and tricuspid AV. Significantly fewer lamellar units and thinner aortic walls were found in surgical samples compared to the reference group regardless of AV morphology, AV function, age, and aortic dilatation. Adventitial structural impairment was associated with stiffer aortic walls. Hints were found that AV morphology (rather than AV function, age, and presence/absence of aortic dilatation) affects structural and functional ascending aortic wall properties. Additionally, the observations suggest more advanced aortic degeneration in association with unicuspid AV, underpin the need for non-surgical control samples in surgical pathological studies, and highlight the importance of the adventitial layer for aortic biomechanics.

主动脉瓣畸形和功能障碍与主动脉壁退变有关。同时评估房室形态、房室功能、年龄、升主动脉扩张和主动脉生物力学特性的研究缺乏。本探索性研究旨在缩小这一差距。对102例升主动脉手术标本进行组织学评估。在超声心动图研究的基础上，计算弹性模量（斜率应力-应变曲线）。从患者病历中收集患者特征。尸检中获得的样本（n=10）作为显微镜分析的参考。采用比较分析和Spearman相关矩阵对患者特征、主动脉壁结构改变和生物力学特性进行统计学探讨。与二尖瓣和三尖瓣AV相比，单尖瓣AV形态在生命早期发现明显的内侧退变。与参考组相比，无论AV形态、AV功能、年龄和主动脉扩张情况如何，手术样本中发现的板层单位明显减少，主动脉壁更薄。动脉外结构损伤与主动脉壁硬化有关。我们发现，房颤形态（而不是房颤功能、年龄和是否有主动脉扩张）影响升主动脉壁的结构和功能特性。此外，观察结果表明，与单尖瓣AV相关的晚期主动脉退变更严重，支持了手术病理研究中对非手术对照样本的需求，并强调了主动脉外膜层生物力学的重要性。

{"title":"Aortic valve morphology rather than aortic valve function, aortic dilatation, and age interferes with ascending aortic structural and biomechanical properties","authors":"Jan M. Federspiel , Jan-Christian Reil , Peter H. Schmidt , Paul Teping , Frank Ramsthaler , Tanja Schwab , Hans-Joachim Schäfers","doi":"10.1016/j.carpath.2025.107782","DOIUrl":"10.1016/j.carpath.2025.107782","url":null,"abstract":"<div><div>Aortic valve (AV) malformation and AV malfunction have been linked to aortic wall degeneration. Studies concomitantly assessing AV morphology, AV function, age, ascending aortic dilatation, and aortic biomechanical properties are lacking. This exploratory study aims to close this gap. Surgical samples of the ascending aorta (n=102) were histologically assessed. Based on echocardiographic studies, the elastic modulus (slope stress-strain curve) was calculated. Patient characteristics were collected from the patient charts. Samples obtained during autopsy (n=10) served as reference for the microscopic analysis. The patient characteristics, structural aortic wall changes, and biomechanical wall properties were statistically explored using comparative analyses and a Spearman correlation matrix. Marked medial degeneration was found significantly earlier in life for unicuspid AV morphology compared to bicuspid and tricuspid AV. Significantly fewer lamellar units and thinner aortic walls were found in surgical samples compared to the reference group regardless of AV morphology, AV function, age, and aortic dilatation. Adventitial structural impairment was associated with stiffer aortic walls. Hints were found that AV morphology (rather than AV function, age, and presence/absence of aortic dilatation) affects structural and functional ascending aortic wall properties. Additionally, the observations suggest more advanced aortic degeneration in association with unicuspid AV, underpin the need for non-surgical control samples in surgical pathological studies, and highlight the importance of the adventitial layer for aortic biomechanics.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107782"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giovanni Maria Lancisi (1654-1720) and the first historical investigation on pathology of sudden death 乔瓦尼·玛丽亚·兰西西（1654-1720）和猝死病理学的第一次历史调查。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-09-19 DOI: 10.1016/j.carpath.2025.107780

Daniela Marrone , Cristina Basso , Gaetano Thiene

Between 1705 and 1706, Rome experienced a series of sudden deaths, prompting Pope Clement XI to set up a commission to investigate the causes. Giovanni Maria Lancisi, a prominent physician, oversaw forensic autopsies and wrote “De subitaneis mortibus” (“On Sudden Deaths”, 1708), a groundbreaking treatise that examined death through a mechanistic lens, offering both theoretical and practical insights. Lancisi’s book presented life as a dynamic interaction of bodily fluids and systems, with death defined as the cessation of these movements. He proposed that sudden death is not rare but a natural endpoint when life-sustaining processes abruptly cease. The treatise identified various causes of sudden death, involving heart and vessels, and debunked fears of an ongoing epidemic in Rome. By analyzing cadaveric lesions, Lancisi demonstrated that these deaths were primarily due to pre-existing morbid conditions. His observations advanced the emerging field of pathological anatomy, applying scientific method on the study of sudden death.

1705年至1706年间，罗马经历了一系列的突发死亡事件，促使教皇克莱门特十一世成立了一个委员会来调查原因。著名医生乔瓦尼·玛丽亚·兰西西（Giovanni Maria Lancisi）监督法医尸检，并撰写了《猝死论》（De subitaneis mortibus, 1708），这是一部开创性的论文，从机械的角度审视死亡，提供了理论和实践的见解。兰西西的书将生命描述为体液和身体系统的动态相互作用，死亡被定义为这些运动的停止。他提出，猝死并不罕见，而是维持生命的过程突然停止时的自然终点。这篇论文指出了猝死的各种原因，涉及心脏和血管，并揭穿了对罗马正在流行的流行病的恐惧。通过对尸体病变的分析，Lancisi证明这些死亡主要是由于先前存在的病态状况。他的观察促进了病理解剖学这一新兴领域的发展，将科学的方法应用于猝死的研究。

引用次数: 0

Circ_0005372 targets the miR-153-3p/ITGB3 axis to stimulate the PI3K/AKT signaling pathway to facilitate the occurrence and development of congenital heart disease and pulmonary arterial hypertension in children Circ_0005372靶向miR-153-3p/ITGB3轴，刺激PI3K/AKT信号通路，促进儿童先天性心脏病和肺动脉高压的发生发展。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-08-27 DOI: 10.1016/j.carpath.2025.107768

Mingyan Zhu , Guixiang Li , Yungui Li , Jinsong Chen

Background

Congenital heart disease (CHD) is the most common and highest incidence of congenital malformations in newborn infants. Pulmonary arterial hypertension (PAH) is the most serious complication associated with CHD. It is a great threat to the safety and health of newborns.

Methods

The expression of circ_0005372, miR-153-3p, and ITGB3 were detected by Real-time quantitative PCR (RT-qPCR) and western blot (WB). MTT and EdU assays were used to evaluate the viability and proliferation of human pulmonary artery smooth muscle cells (HPASMC). Migration and invasion abilities were determined by wound healing and Transwell assays. Pull down and RNA Immunoprecipitation (RIP), and dual luciferase reporter gene assays were used to detect targeting relationships of circ_0005372, miR-153-3p, and ITGB3.

Results

Plasma samples from 27 healthy, CHD, and PAHCHD pairs of newborns were collected. Circ_0005372 was highly expressed in PAHCHD patient samples and HPASMCs under hypoxic conditions. Knockdown of circ_0005372 inhibited HPASMCs viability, proliferation, migration, and invasion. Circ_0005372 targeted miR-153-3p and showed a negative correlation. The inhibitory effect of silencing circ_0005372 on the HPASMCs was reversed by miR-153-3p inhibitor. MiR-153-3p negatively regulated ITGB3, and overexpression of ITGB3 abolished the effect of miR-153-3p in hypoxia-treated HPASMCs. Circ_0005372 could regulate the PI3K/AKT signaling pathway through miR-153-3p/ ITGB3.

Conclusion

In summary, circ_0005372 mediates the expression of ITG3B via targeting miR-153-3p, thereby stimulating the PI3K/AKT signaling pathway, helping the proliferation, migration, and invasion of HPASMCs and accelerating the deterioration of PAHCHD.

背景：先天性心脏病（CHD）是新生儿中最常见、发病率最高的先天性畸形。肺动脉高压（PAH）是冠心病最严重的并发症。这是对新生儿安全和健康的巨大威胁。方法：采用实时荧光定量PCR （RT-qPCR）和western blot （WB）检测circ_0005372、miR-153-3p、ITGB3的表达。采用MTT和EdU测定人肺动脉平滑肌细胞（HPASMC）的活力和增殖能力。通过伤口愈合和Transwell试验测定迁移和侵袭能力。使用Pull - down和RNA免疫沉淀（RIP）以及双荧光素酶报告基因检测circ_0005372、miR-153-3p和ITGB3的靶向关系。结果：收集了27对健康、冠心病和PAH-CHD新生儿的血浆样本。Circ_0005372在缺氧条件下的PAH-CHD患者样本和HPASMCs中高表达。敲低circ_0005372抑制HPASMCs的活力、增殖、迁移和侵袭。Circ_0005372靶向miR-153-3p，呈负相关。沉默circ_0005372对HPASMCs的抑制作用被miR-153-3p抑制剂逆转。MiR-153-3p负性调节ITGB3， ITGB3过表达可消除MiR-153-3p在缺氧处理的HPASMCs中的作用。Circ_0005372可通过miR-153-3p/ ITGB3调控PI3K/AKT信号通路。结论：综上所述，circ_0005372通过靶向miR-153-3p介导ITG3B的表达，从而刺激PI3K/AKT信号通路，促进hpasmc的增殖、迁移和侵袭，加速PAH-CHD的恶化。

{"title":"Circ_0005372 targets the miR-153-3p/ITGB3 axis to stimulate the PI3K/AKT signaling pathway to facilitate the occurrence and development of congenital heart disease and pulmonary arterial hypertension in children","authors":"Mingyan Zhu , Guixiang Li , Yungui Li , Jinsong Chen","doi":"10.1016/j.carpath.2025.107768","DOIUrl":"10.1016/j.carpath.2025.107768","url":null,"abstract":"<div><h3>Background</h3><div>Congenital heart disease (CHD) is the most common and highest incidence of congenital malformations in newborn infants. Pulmonary arterial hypertension (PAH) is the most serious complication associated with CHD. It is a great threat to the safety and health of newborns.</div></div><div><h3>Methods</h3><div>The expression of circ_0005372, miR-153-3p, and ITGB3 were detected by Real-time quantitative PCR (RT-qPCR) and western blot (WB). MTT and EdU assays were used to evaluate the viability and proliferation of human pulmonary artery smooth muscle cells (HPASMC). Migration and invasion abilities were determined by wound healing and Transwell assays. Pull down and RNA Immunoprecipitation (RIP), and dual luciferase reporter gene assays were used to detect targeting relationships of circ_0005372, miR-153-3p, and ITGB3.</div></div><div><h3>Results</h3><div>Plasma samples from 27 healthy, CHD, and PAH<img>CHD pairs of newborns were collected. Circ_0005372 was highly expressed in PAH<img>CHD patient samples and HPASMCs under hypoxic conditions. Knockdown of circ_0005372 inhibited HPASMCs viability, proliferation, migration, and invasion. Circ_0005372 targeted miR-153-3p and showed a negative correlation. The inhibitory effect of silencing circ_0005372 on the HPASMCs was reversed by miR-153-3p inhibitor. MiR-153-3p negatively regulated ITGB3, and overexpression of ITGB3 abolished the effect of miR-153-3p in hypoxia-treated HPASMCs. Circ_0005372 could regulate the PI3K/AKT signaling pathway through miR-153-3p/ ITGB3.</div></div><div><h3>Conclusion</h3><div>In summary, circ_0005372 mediates the expression of ITG3B via targeting miR-153-3p, thereby stimulating the PI3K/AKT signaling pathway, helping the proliferation, migration, and invasion of HPASMCs and accelerating the deterioration of PAH<img>CHD.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107768"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aortic wall lamellar structure in phylogeny and in humans: insights from bicuspid and tricuspid aortic valve morphology 系统发育和人类的主动脉壁板层结构：来自二尖瓣和三尖瓣主动脉瓣形态的见解。

IF 1.9 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Pathology

Pub Date : 2026-01-01 Epub Date: 2025-10-31 DOI: 10.1016/j.carpath.2025.107789

Nora Bacour , Mohammad Zafar , Nicole Kargin , Bulat Zinganshin , Robert Poelmann , Robert J.M. Klautz , John Elefteriades , Nimrat Grewal

Objective

Despite a voluminous literature on aortopathy, comparatively little attention has been paid to the lamellar architecture of the aortic wall, including the number and distribution of these layers. This study compares the lamellar organization of the developing aorta in individuals with a bicuspid (BAV) versus tricuspid aortic valve (TAV) and includes a literature review on aortic lamellae in mammals and adult humans.

Methods

Non-dilated ascending aortic wall samples (n = 83) were analyzed from individuals aged embryonic to 72 years, categorized into six age groups. Additionally, a literature review was carried out on lamellar counts across species.

Results

The elastic lamellae in preterm aortas were well-structured with no pathological features. Conversely, adult aortas showed progressive elastic fiber pathology. The number of lamellae increased with age; neonates had the lowest count, peaking in young children, and gradually decreasing in adolescents. Furthermore, compared to TAV patients, BAV patients showed patterns of thinner intimal layers and fewer elastic lamellae. Our literature review showed that the quantity of lamellae in mammals is correlated with both body size and aortic radius, with smaller animals having fewer lamellae. The ascending thoracic aorta in humans had between 45 and 78 elastic lamellae, while the descending and abdominal aortas had fewer layers.

Conclusion

The number of elastic lamellae in the aortic wall is influenced by both age and valve morphology. BAV patients had less lamellae than TAV patients, which could contribute to the disparity in clinical outcomes. In older adults, age-related lamellar degeneration likely increases the risk of aortopathy.

目的：尽管有大量关于主动脉病变的文献，但相对较少关注主动脉壁的板层结构，包括这些层的数量和分布。本研究比较了患有二尖瓣（BAV）和三尖瓣主动脉瓣（TAV）的个体的主动脉板层组织，并对哺乳动物和成人主动脉板层的文献进行了综述。方法：对83例未扩张的升主动脉壁样本进行分析，这些样本来自胚胎至72岁的个体，分为6个年龄组。此外，还对不同物种间的片层数进行了文献综述。结果：早产儿主动脉弹性片结构完整，无明显病理特征。相反，成人主动脉表现为进行性弹性纤维病变。随着年龄增长，叶片数量增加；新生儿的计数最低，在幼儿中达到峰值，在青少年中逐渐减少。此外，与TAV患者相比，BAV患者表现出更薄的内膜层和更少的弹性片层。我们的文献综述表明，哺乳动物的瓣片数量与体型和主动脉半径相关，体型越小的动物瓣片越少。人类的胸升主动脉有45到78个弹性薄片，而降主动脉和腹主动脉的层数更少。结论：主动脉壁弹性薄片的数量受年龄和瓣膜形态的影响。BAV患者片层少于TAV患者，这可能导致临床结果的差异。在老年人中，年龄相关性板层变性可能增加主动脉病变的风险。

{"title":"Aortic wall lamellar structure in phylogeny and in humans: insights from bicuspid and tricuspid aortic valve morphology","authors":"Nora Bacour , Mohammad Zafar , Nicole Kargin , Bulat Zinganshin , Robert Poelmann , Robert J.M. Klautz , John Elefteriades , Nimrat Grewal","doi":"10.1016/j.carpath.2025.107789","DOIUrl":"10.1016/j.carpath.2025.107789","url":null,"abstract":"<div><h3>Objective</h3><div>Despite a voluminous literature on aortopathy, comparatively little attention has been paid to the lamellar architecture of the aortic wall, including the number and distribution of these layers. This study compares the lamellar organization of the developing aorta in individuals with a bicuspid (BAV) versus tricuspid aortic valve (TAV) and includes a literature review on aortic lamellae in mammals and adult humans.</div></div><div><h3>Methods</h3><div>Non-dilated ascending aortic wall samples (<em>n</em> = 83) were analyzed from individuals aged embryonic to 72 years, categorized into six age groups. Additionally, a literature review was carried out on lamellar counts across species.</div></div><div><h3>Results</h3><div>The elastic lamellae in preterm aortas were well-structured with no pathological features. Conversely, adult aortas showed progressive elastic fiber pathology. The number of lamellae increased with age; neonates had the lowest count, peaking in young children, and gradually decreasing in adolescents. Furthermore, compared to TAV patients, BAV patients showed patterns of thinner intimal layers and fewer elastic lamellae. Our literature review showed that the quantity of lamellae in mammals is correlated with both body size and aortic radius, with smaller animals having fewer lamellae. The ascending thoracic aorta in humans had between 45 and 78 elastic lamellae, while the descending and abdominal aortas had fewer layers.</div></div><div><h3>Conclusion</h3><div>The number of elastic lamellae in the aortic wall is influenced by both age and valve morphology. BAV patients had less lamellae than TAV patients, which could contribute to the disparity in clinical outcomes. In older adults, age-related lamellar degeneration likely increases the risk of aortopathy.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107789"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145430418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Cardiovascular Pathology

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀