首页 > 最新文献

Cancer Research and Treatment : Official Journal of Korean Cancer Association最新文献

英文 中文
Ovarian, Fallopian Tube, and Primary Peritoneal Cancer 卵巢癌、输卵管癌和原发性腹膜癌
Pub Date : 2020-10-31 DOI: 10.1201/9780429026638-19
R. Morgan, A. Clamp, G. Jayson
{"title":"Ovarian, Fallopian Tube, and Primary Peritoneal Cancer","authors":"R. Morgan, A. Clamp, G. Jayson","doi":"10.1201/9780429026638-19","DOIUrl":"https://doi.org/10.1201/9780429026638-19","url":null,"abstract":"","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87252792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Renal Cell Cancer 肾细胞癌
Pub Date : 2020-10-31 DOI: 10.1201/9780429026638-18
S. Ellis, J. Hack
{"title":"Renal Cell Cancer","authors":"S. Ellis, J. Hack","doi":"10.1201/9780429026638-18","DOIUrl":"https://doi.org/10.1201/9780429026638-18","url":null,"abstract":"","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82792127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural Plants and Cancer Therapy 天然植物和癌症治疗
Ahmed M. Kabel
Cancer chemotherapy represents the cornerstone of management of a vast majority of neoplastic lesions. Adverse reactions of chemotherapy may affect the major body organs and represent deleterious health hazards for cancer patients. Recent studies are directed towards discovery of natural compounds of plant origin that have potential anticancer properties and may ameliorate the toxic effects induced by the traditional anticancer agents. This manuscript throws light on the potential role of the natural plants in cancer therapy.
癌症化疗是绝大多数肿瘤病变治疗的基石。化疗不良反应可能影响肿瘤患者的主要身体器官,对肿瘤患者的健康构成有害危害。最近的研究是针对发现植物来源的天然化合物,具有潜在的抗癌特性,并可能改善由传统抗癌药物引起的毒性作用。这篇手稿揭示了天然植物在癌症治疗中的潜在作用。
{"title":"Natural Plants and Cancer Therapy","authors":"Ahmed M. Kabel","doi":"10.12691/JCRT-8-2-5","DOIUrl":"https://doi.org/10.12691/JCRT-8-2-5","url":null,"abstract":"Cancer chemotherapy represents the cornerstone of management of a vast majority of neoplastic lesions. Adverse reactions of chemotherapy may affect the major body organs and represent deleterious health hazards for cancer patients. Recent studies are directed towards discovery of natural compounds of plant origin that have potential anticancer properties and may ameliorate the toxic effects induced by the traditional anticancer agents. This manuscript throws light on the potential role of the natural plants in cancer therapy.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87422395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anxiety and Pain Level Associated with Mammography and the Impact of the Preexistence of Knowledge 与乳房x光检查相关的焦虑和疼痛水平以及知识前生的影响
F. Aldhafeeri, M. Abozeed
Background: Breast cancer is the most common type of cancer in women, after skin cancer. Early detection and treatment are recommended for reducing mortality and suffering. However, screening behaviors are often avoided for many reasons, such as anxiety and stress. Mammography screening is the main recommended test for the early detection of breast cancer. The objective of this study was to assess the knowledge, level of pain, and stress in women during the early screening of breast cancer with a mammogram. Materials and methods: This study has been approved by the Local Research Ethics Committee. The study was conducted at Hafr Elbatin Central Hospital in Saudi Arabi among 100 women. Data were collected via a structured interview questionnaire before and immediately after the mammography procedure. The questionnaire consisted of three parts: (1) sociodemographic data and knowledge about the mammogram examination, (2) pain scale, and (3) anxiety scale. Data were entered and analyzed using SPSS version 25. Results: A total of 75% (n=75) of the women reported moderate to severe pain during the mammogram examination. Up to 57% (n=57) of the women expressed severe anxiety about the mammogram procedure. Most of the women-85% (n=85)-were found to have poor knowledge related to mammograms and mammogram preparation. There was a strong correlation between pain before and during the mammogram procedure, P-Value (0.00001*) with Chi-squared (33.40) and a highly significant correlation between satisfactory and unsatisfactory knowledge in women's P-Value (0.00001*) with z test (6.57). A poor knowledge about breast cancer was detected in the overall studied women and those who had never undergone mammography, particularly knowledge related to the risk factors for breast cancer. The most important predictors of the barriers to mammography were incorrect beliefs about mammography and its procedures. Conclusion: Pain expectations can be approached in various ways to make the mammography experience much more tolerable for women, thereby encouraging them to attend and return for their scans. It was determined that women who had mammography had a moderate level of anxiety.
背景:乳腺癌是女性中最常见的癌症类型,仅次于皮肤癌。建议及早发现和治疗,以减少死亡率和痛苦。然而,由于焦虑和压力等原因,人们往往会避免筛查行为。乳房x光检查是早期发现乳腺癌的主要推荐检查。本研究的目的是评估妇女在早期乳房x光检查中对乳腺癌的认知、疼痛程度和压力。材料和方法:本研究已获得当地研究伦理委员会批准。这项研究是在沙特阿拉伯的哈弗埃尔巴廷中心医院对100名妇女进行的。数据通过乳房x光检查前后的结构化访谈问卷收集。问卷由三个部分组成:(1)社会人口学资料和乳房x线检查知识;(2)疼痛量表;(3)焦虑量表。数据输入和分析使用SPSS版本25。结果:共有75% (n=75)的女性在乳房x光检查期间报告中度至重度疼痛。高达57% (n=57)的女性对乳房x光检查表示严重焦虑。85% (n=85)的大多数妇女对乳房x光检查和乳房x光检查准备知识贫乏。乳房x线检查前和检查中疼痛的p值(0.00001*)与卡方(33.40)有很强的相关性,女性的p值(0.00001*)与z检验(6.57)有极显著的相关性。在所有被研究的女性和从未接受过乳房x光检查的女性中发现,她们对乳腺癌的了解很少,尤其是对乳腺癌危险因素的了解很少。乳房x光检查障碍最重要的预测因素是对乳房x光检查及其程序的错误信念。结论:疼痛预期可以通过多种方式达到,使女性更能忍受乳房x光检查的经历,从而鼓励她们参加并返回进行扫描。经确定,接受乳房x光检查的女性有中等程度的焦虑。
{"title":"Anxiety and Pain Level Associated with Mammography and the Impact of the Preexistence of Knowledge","authors":"F. Aldhafeeri, M. Abozeed","doi":"10.12691/jcrt-8-2-4","DOIUrl":"https://doi.org/10.12691/jcrt-8-2-4","url":null,"abstract":"Background: Breast cancer is the most common type of cancer in women, after skin cancer. Early detection and treatment are recommended for reducing mortality and suffering. However, screening behaviors are often avoided for many reasons, such as anxiety and stress. Mammography screening is the main recommended test for the early detection of breast cancer. The objective of this study was to assess the knowledge, level of pain, and stress in women during the early screening of breast cancer with a mammogram. Materials and methods: This study has been approved by the Local Research Ethics Committee. The study was conducted at Hafr Elbatin Central Hospital in Saudi Arabi among 100 women. Data were collected via a structured interview questionnaire before and immediately after the mammography procedure. The questionnaire consisted of three parts: (1) sociodemographic data and knowledge about the mammogram examination, (2) pain scale, and (3) anxiety scale. Data were entered and analyzed using SPSS version 25. Results: A total of 75% (n=75) of the women reported moderate to severe pain during the mammogram examination. Up to 57% (n=57) of the women expressed severe anxiety about the mammogram procedure. Most of the women-85% (n=85)-were found to have poor knowledge related to mammograms and mammogram preparation. There was a strong correlation between pain before and during the mammogram procedure, P-Value (0.00001*) with Chi-squared (33.40) and a highly significant correlation between satisfactory and unsatisfactory knowledge in women's P-Value (0.00001*) with z test (6.57). A poor knowledge about breast cancer was detected in the overall studied women and those who had never undergone mammography, particularly knowledge related to the risk factors for breast cancer. The most important predictors of the barriers to mammography were incorrect beliefs about mammography and its procedures. Conclusion: Pain expectations can be approached in various ways to make the mammography experience much more tolerable for women, thereby encouraging them to attend and return for their scans. It was determined that women who had mammography had a moderate level of anxiety.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85878726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Pituitary Adenomas: Insights into the Recent Trends 垂体腺瘤:对近期趋势的洞察
Ahmed M. Kabel
Pituitary adenoma is a type of pituitary neoplasms that is typically a benign, slow-growing tumor that arises from cells in the pituitary gland. Pituitary adenomas are classified according to their secretory products into functioning (Endocrine-active) tumors that represent almost 70% of pituitary tumors, and non-functioning adenomas (Endocrine-inactive). Because of physiologic effects of excess hormones, the functioning tumors present earlier than non-functioning adenomas. Patients with pituitary adenomas usually present with symptoms related to hormonal disturbances. However, pressure symptoms, such as headache, visual field defects, cranial nerve palsies and hypopituitarism may occur due to the mass effect of large tumors. Treatment of pituitary adenomas include transsphenoidal surgery and adjunctive therapy including supervoltage radiation and pharmacologic agents. This review throws light on pituitary adenoma regarding its prevalence, predisposing factors, types, clinical presentation, diagnosis and possible lines of management in view of the recent trends.
垂体腺瘤是一种典型的良性、生长缓慢的肿瘤,起源于垂体细胞。垂体腺瘤根据其分泌产物分为功能性(内分泌活性)肿瘤(约占垂体肿瘤的70%)和非功能性腺瘤(内分泌非活性)。由于过量激素的生理作用,功能肿瘤比无功能腺瘤更早出现。垂体腺瘤患者通常表现出与激素紊乱相关的症状。然而,由于大肿瘤的肿块效应,可能会出现头痛、视野缺损、脑神经麻痹和垂体功能减退等压力症状。垂体腺瘤的治疗包括经蝶窦手术和辅助治疗,包括超电压辐射和药物治疗。本文综述了垂体腺瘤的流行、易感因素、类型、临床表现、诊断和可能的治疗方法。
{"title":"Pituitary Adenomas: Insights into the Recent Trends","authors":"Ahmed M. Kabel","doi":"10.12691/jcrt-8-2-3","DOIUrl":"https://doi.org/10.12691/jcrt-8-2-3","url":null,"abstract":"Pituitary adenoma is a type of pituitary neoplasms that is typically a benign, slow-growing tumor that arises from cells in the pituitary gland. Pituitary adenomas are classified according to their secretory products into functioning (Endocrine-active) tumors that represent almost 70% of pituitary tumors, and non-functioning adenomas (Endocrine-inactive). Because of physiologic effects of excess hormones, the functioning tumors present earlier than non-functioning adenomas. Patients with pituitary adenomas usually present with symptoms related to hormonal disturbances. However, pressure symptoms, such as headache, visual field defects, cranial nerve palsies and hypopituitarism may occur due to the mass effect of large tumors. Treatment of pituitary adenomas include transsphenoidal surgery and adjunctive therapy including supervoltage radiation and pharmacologic agents. This review throws light on pituitary adenoma regarding its prevalence, predisposing factors, types, clinical presentation, diagnosis and possible lines of management in view of the recent trends.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90615151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
VEGF and KRAS are Potential Targets of miR-206 Modulation in Triple Negative Breast Cancer VEGF和KRAS是miR-206在三阴性乳腺癌中调节的潜在靶点
Shaymaa E. El Feky, Fawziya A. R. Ibrahim, A. Nassar, N. A. E. Moneim, S. A. Ebeid, Mohammad Ahmad, Sanaa Shawky, Mohammad M. Nasef
Triple negative is a subtype of breast cancer characterized by lack of expression of hormone receptors (ER, PR and Her2/neu). Due to the limited treatment options, the search for novel treatment targets continues. The aim of this study was to assess the differential expression of miR-206, VEGF and KRAS in TNBC and non-TNBC tissues and cell lines and to evaluate the modulatory effect of miR-206 on the key oncogenic targets VEGF and KRAS. The expression of miR-206, VEGF and KRAS was quantified using real time PCR in both paraffin embedded breast cancer and adjacent tissues as well as in MDA-MB-231 and MCF-7 cell lines. Cell lines were transfected with different concentrations of miR-206 mimic and their viability were assessed using MTT assay. Our results indicated that miR-206 was significantly downregulated in cancerous compared to non-cancerous tissues with a more pronounced downregulation in TNBC than non-TNBC tissues. VEGF and KRAS were significantly upregulated in TNBC compared to non-TNBC and their expression was negatively correlated to miR-206 expression. Transfection of TNBC and non-TNBC cell lines with miR-206 mimic resulted in a dose dependent reduction in cell viability as well as a significant reduction in VEGF and KRAS expression. In conclusion, based on our combined human tissues and cell line-based investigations we can suggest that VEGF and KRAS may be potential targets for miR-206-mediated regulation and that their targeting by miR-206 can be a highly efficient therapeutic strategy in TNBC.
三阴性是一种以缺乏激素受体(ER、PR和Her2/neu)表达为特征的乳腺癌亚型。由于治疗方案有限,寻找新的治疗靶点仍在继续。本研究的目的是评估miR-206、VEGF和KRAS在TNBC和非TNBC组织细胞系中的差异表达,并评估miR-206对关键致癌靶点VEGF和KRAS的调节作用。采用real - time PCR定量检测乳腺癌石蜡包埋组织及癌旁组织、MDA-MB-231和MCF-7细胞系中miR-206、VEGF和KRAS的表达。用不同浓度的miR-206模拟物转染细胞系,并使用MTT法评估其生存能力。我们的研究结果表明,miR-206在癌变组织中比在非癌变组织中显著下调,在TNBC中比在非TNBC组织中下调更为明显。与非TNBC相比,TNBC中VEGF和KRAS的表达显著上调,且其表达与miR-206表达呈负相关。用miR-206模拟物转染TNBC和非TNBC细胞系导致细胞活力的剂量依赖性降低,以及VEGF和KRAS表达的显著降低。总之,基于我们基于人体组织和细胞系的联合研究,我们可以提出VEGF和KRAS可能是miR-206介导的调控的潜在靶点,并且miR-206靶向它们可能是TNBC的高效治疗策略。
{"title":"VEGF and KRAS are Potential Targets of miR-206 Modulation in Triple Negative Breast Cancer","authors":"Shaymaa E. El Feky, Fawziya A. R. Ibrahim, A. Nassar, N. A. E. Moneim, S. A. Ebeid, Mohammad Ahmad, Sanaa Shawky, Mohammad M. Nasef","doi":"10.12691/jcrt-7-1-2","DOIUrl":"https://doi.org/10.12691/jcrt-7-1-2","url":null,"abstract":"Triple negative is a subtype of breast cancer characterized by lack of expression of hormone receptors (ER, PR and Her2/neu). Due to the limited treatment options, the search for novel treatment targets continues. The aim of this study was to assess the differential expression of miR-206, VEGF and KRAS in TNBC and non-TNBC tissues and cell lines and to evaluate the modulatory effect of miR-206 on the key oncogenic targets VEGF and KRAS. The expression of miR-206, VEGF and KRAS was quantified using real time PCR in both paraffin embedded breast cancer and adjacent tissues as well as in MDA-MB-231 and MCF-7 cell lines. Cell lines were transfected with different concentrations of miR-206 mimic and their viability were assessed using MTT assay. Our results indicated that miR-206 was significantly downregulated in cancerous compared to non-cancerous tissues with a more pronounced downregulation in TNBC than non-TNBC tissues. VEGF and KRAS were significantly upregulated in TNBC compared to non-TNBC and their expression was negatively correlated to miR-206 expression. Transfection of TNBC and non-TNBC cell lines with miR-206 mimic resulted in a dose dependent reduction in cell viability as well as a significant reduction in VEGF and KRAS expression. In conclusion, based on our combined human tissues and cell line-based investigations we can suggest that VEGF and KRAS may be potential targets for miR-206-mediated regulation and that their targeting by miR-206 can be a highly efficient therapeutic strategy in TNBC.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80177805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Astrocytoma: Insights into Risk Factors, Pathogenesis, Diagnosis and Management 星形细胞瘤:危险因素、发病机制、诊断和治疗
Ahmed M. Kabel, Kholoud Modais, A. Salim, R. Ahmad, A. Ahmad, Khaled A. Alnumari
Astrocytoma is one of the most common types of brain tumors. It arises from astrocytes-star-shaped cells that make up the i°glue-likei± or supportive tissue of the brain. There are different types and severities of Astrocytomas. According to (WHO),It can be classified into grades from I to IV. The most frequently diagnosed types of astrocytoma are pilocytic astrocytoma, grade II astrocytoma, anaplastic astrocytoma and glioblastoma multiforme. They may or may not be cancerous. The exact cause of astrocytoma is unknown. Astrocytomas occur slightly more often in men than in women. They are slightly more common in Caucasians. Astrocytomas can develop in both children and adults. The diagnosis of astrocytoma is based on a thorough clinical evaluation, characteristic physical findings, a careful patient history, and specialized tests. Astrocytomas may be treated with surgery, radiation therapy, chemotherapy or a combination of treatments.
星形细胞瘤是最常见的脑肿瘤之一。它起源于星形细胞,星形细胞构成了大脑的胶质样组织或支持组织。星形细胞瘤有不同的类型和严重程度。根据WHO,星形细胞瘤可分为I ~ IV级,诊断最多的类型为毛细胞星形细胞瘤、II级星形细胞瘤、间变性星形细胞瘤和多形性胶质母细胞瘤。它们可能是癌细胞,也可能不是。星形细胞瘤的确切病因尚不清楚。星形细胞瘤在男性中的发生率略高于女性。它们在白种人中更为常见。星形细胞瘤可在儿童和成人中发生。星形细胞瘤的诊断是基于彻底的临床评估、特征性的物理表现、仔细的患者病史和专门的检查。星形细胞瘤可以通过手术、放射治疗、化疗或综合治疗来治疗。
{"title":"Astrocytoma: Insights into Risk Factors, Pathogenesis, Diagnosis and Management","authors":"Ahmed M. Kabel, Kholoud Modais, A. Salim, R. Ahmad, A. Ahmad, Khaled A. Alnumari","doi":"10.12691/JCRT-6-3-2","DOIUrl":"https://doi.org/10.12691/JCRT-6-3-2","url":null,"abstract":"Astrocytoma is one of the most common types of brain tumors. It arises from astrocytes-star-shaped cells that make up the i°glue-likei± or supportive tissue of the brain. There are different types and severities of Astrocytomas. According to (WHO),It can be classified into grades from I to IV. The most frequently diagnosed types of astrocytoma are pilocytic astrocytoma, grade II astrocytoma, anaplastic astrocytoma and glioblastoma multiforme. They may or may not be cancerous. The exact cause of astrocytoma is unknown. Astrocytomas occur slightly more often in men than in women. They are slightly more common in Caucasians. Astrocytomas can develop in both children and adults. The diagnosis of astrocytoma is based on a thorough clinical evaluation, characteristic physical findings, a careful patient history, and specialized tests. Astrocytomas may be treated with surgery, radiation therapy, chemotherapy or a combination of treatments.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76086108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Pancreatic Cancer 胰腺癌
Pub Date : 2018-08-11 DOI: 10.1201/9781315379678-18
K. Mann, A. Sheel, P. Ghaneh
{"title":"Pancreatic Cancer","authors":"K. Mann, A. Sheel, P. Ghaneh","doi":"10.1201/9781315379678-18","DOIUrl":"https://doi.org/10.1201/9781315379678-18","url":null,"abstract":"","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89064125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anticancer Effect of Medicinal Mushroom with Prooxidant Activity on Human Bladder Cancer Cells 具有抗氧化活性的药菇对人膀胱癌细胞的抗癌作用
C. Fox, R. Yau, M. Choudhury, J. Phillips, S. Konno
Oxidative stress (OXS) has been recently considered as one of anticancer strategies by taking advantage of higher vulnerability of cancer cells (than normal cells) to OXS. In fact, the successful outcomes using OXS have been reported in several cancer cases. A medicinal mushroom extract, PE isolated from Poria mushroom, has been shown to have anticancer/antitumor activity, although its anticancer mechanism has not been fully understood but may involve OXS. We investigated if PE might have anticancer effect on human bladder cancer cells through OXS in vitro. A dose-dependent (0-200 g/ml of PE) study was first performed to assess cell viability using MTT assay. PE led to a significant reduction in cell viability with the IC50 (50% inhibitory concentration) of 100 g/ml. A possible anticancer role of OXS was then assessed by lipid peroxidation (LPO) assay. The results indicated that PE indeed exerted ~2.1-fold greater OXS (than controls) on the cells. The anticancer mechanism of PE was further explored, focusing on glycolysis, metabolic signaling pathways, and apoptosis. Two glycolytic parameters, hexokinase (HK) activity and cellular ATP level, have significantly declined, suggesting the inhibition of glycolysis. Coupled with the reduced ATP level, AMP-activated protein kinase (AMPK) was activated, while protein kinase B (Akt) was inactivated and concomitantly mammalian target of rapamycin (mTOR) was inhibited. These results imply the growth cessation, followed by cell death. Western blot analysis also revealed that such cell death was more likely linked to apoptosis, indicated by the bcl-2 down-regulation and the Bax up-regulation. Therefore, PE is a natural anticancer agent with prooxidant activity exerting OXS, which leads to inhibition of glycolysis, modulations of metabolic signaling pathways, and ultimately apoptosis. It may have clinical implications in oral and/or intravesical administration for a safer and better therapeutic option for bladder cancer.
氧化应激(OXS)是利用癌细胞(比正常细胞)对氧化应激具有更高的易变性而被认为是抗癌策略之一。事实上,在一些癌症病例中已经报道了使用OXS的成功结果。从茯苓中分离的药用蘑菇提取物PE已被证明具有抗癌/抗肿瘤活性,尽管其抗癌机制尚未完全了解,但可能与OXS有关。我们通过体外OXS研究PE是否对人膀胱癌细胞有抗癌作用。首先进行剂量依赖性(0-200 g/ml PE)研究,使用MTT法评估细胞活力。PE导致细胞活力显著降低,IC50(50%抑制浓度)为100 g/ml。然后通过脂质过氧化(LPO)试验评估OXS可能的抗癌作用。结果表明,PE对细胞的OXS作用确实比对照组大2.1倍。进一步探讨PE的抗癌机制,重点关注糖酵解、代谢信号通路和细胞凋亡。糖酵解的两个参数己糖激酶(HK)活性和细胞ATP水平显著下降,提示糖酵解受到抑制。随着ATP水平的降低,amp激活的蛋白激酶(AMPK)被激活,而蛋白激酶B (Akt)被失活,同时雷帕霉素靶蛋白(mTOR)被抑制。这些结果暗示生长停止,随后是细胞死亡。Western blot分析显示,bcl-2下调,Bax上调,表明细胞死亡更可能与凋亡有关。因此,PE是一种天然的抗癌剂,具有促氧化活性,发挥OXS作用,从而抑制糖酵解,调节代谢信号通路,最终导致细胞凋亡。它可能对口服和/或膀胱内给药作为膀胱癌更安全和更好的治疗选择具有临床意义。
{"title":"Anticancer Effect of Medicinal Mushroom with Prooxidant Activity on Human Bladder Cancer Cells","authors":"C. Fox, R. Yau, M. Choudhury, J. Phillips, S. Konno","doi":"10.12691/JCRT-6-2-5","DOIUrl":"https://doi.org/10.12691/JCRT-6-2-5","url":null,"abstract":"Oxidative stress (OXS) has been recently considered as one of anticancer strategies by taking advantage of higher vulnerability of cancer cells (than normal cells) to OXS. In fact, the successful outcomes using OXS have been reported in several cancer cases. A medicinal mushroom extract, PE isolated from Poria mushroom, has been shown to have anticancer/antitumor activity, although its anticancer mechanism has not been fully understood but may involve OXS. We investigated if PE might have anticancer effect on human bladder cancer cells through OXS in vitro. A dose-dependent (0-200 g/ml of PE) study was first performed to assess cell viability using MTT assay. PE led to a significant reduction in cell viability with the IC50 (50% inhibitory concentration) of 100 g/ml. A possible anticancer role of OXS was then assessed by lipid peroxidation (LPO) assay. The results indicated that PE indeed exerted ~2.1-fold greater OXS (than controls) on the cells. The anticancer mechanism of PE was further explored, focusing on glycolysis, metabolic signaling pathways, and apoptosis. Two glycolytic parameters, hexokinase (HK) activity and cellular ATP level, have significantly declined, suggesting the inhibition of glycolysis. Coupled with the reduced ATP level, AMP-activated protein kinase (AMPK) was activated, while protein kinase B (Akt) was inactivated and concomitantly mammalian target of rapamycin (mTOR) was inhibited. These results imply the growth cessation, followed by cell death. Western blot analysis also revealed that such cell death was more likely linked to apoptosis, indicated by the bcl-2 down-regulation and the Bax up-regulation. Therefore, PE is a natural anticancer agent with prooxidant activity exerting OXS, which leads to inhibition of glycolysis, modulations of metabolic signaling pathways, and ultimately apoptosis. It may have clinical implications in oral and/or intravesical administration for a safer and better therapeutic option for bladder cancer.","PeriodicalId":9541,"journal":{"name":"Cancer Research and Treatment : Official Journal of Korean Cancer Association","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79039755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
Cancer Research and Treatment : Official Journal of Korean Cancer Association
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1