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The protease-resistant core of the reaction centre protein of photosystem I (P700 Chla-P1) 光系统I反应中心蛋白P700 Chla-P1的蛋白酶抗性核心
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02904437
U. Hinz, D. Simpson
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引用次数: 2
“Light-derepressible” genes are regulated by metal-protein complexes: A hypothesis “光抑制”基因是由金属-蛋白质复合物调控的:一个假设
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02908412
J. Keneth Hoober
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引用次数: 0
Onion-like off-flavour in beer: Isolation and identification of the culprits 啤酒中洋葱样异味:罪魁祸首的分离与鉴定
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02908410
A. Olsén, B. W. Christensen, J. Madsen
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引用次数: 19
Chromosome pairing and synaptonemal complex formation in hexaploid wheat, monosomic for chromosome 5B 小麦六倍体染色体配对和突触复合体的形成，染色体5B为单体
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02907174
P. Holm
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引用次数: 13
In vitro transcription and translation of the psbD gene encoding the D-2 protein of photosystem II in barley. 大麦光系统II D-2蛋白编码基因psbD的体外转录和翻译。
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02904467
E M Neumann

The plastoquinone-binding polypeptide D-2 of the reaction center complex of photosystem II in barley has been expressed in vitro using the expression vector pGem3 containing the psbD gene of chloroplast DNA as template. The full length D-2 polypeptide with an apparent mol. weight of 32 kD is a major product when mRNA is transcribed with SP6 RNA polymerase from an insert containing the psbD gene with a 36 bp 5' leader sequence and a 3' tail of 99 bp downstream of the stop codon. Translation of the mRNA had to be done in a rabbit reticulocyte lysate. Translation also occurred from the internal AUG codons giving rise to truncated D-2 polypeptides with sizes of 30, 25 and 17 kD. They are immunoprecipitable with antisera either raised against amino acid residues 235 to 241 or against 345 residues of the D-2 polypeptide. A truncated translation product of 12 kD probably initiated at codon 247 is not recognized by either antiserum. An additional immunoprecipitable protein with an apparent molecular weight of 46 kD is observed by SDS-PAGE and is interpreted as a homomeric aggregate of D-2 polypeptides. Upon addition of methanol solubilized 2,6 dichloro-p-benzoquinone or other quinones a preferential translation of the full-length and truncated D-2 polypeptides is observed. The use of in vitro synthesized D-2 polypeptides for studies of binding quinones, other electron carriers and chlorophyll chromophores is discussed.

以含叶绿体DNA psbD基因的表达载体pGem3为模板，体外表达了大麦光系统II反应中心复合物的质体醌结合多肽D-2。用SP6 RNA聚合酶从含有psbD基因的插入物中转录mRNA时，主要产物是全长D-2多肽，表观分子量为32 kD，该插入物具有36bp的5'先导序列和99bp的3'尾，位于停止密码子下游。mRNA的翻译必须在兔网织细胞裂解液中完成。内部AUG密码子也发生翻译，产生长度为30,25和17kd的截断的D-2多肽。对D-2多肽的235 ~ 241个氨基酸残基或345个氨基酸残基的抗血清均可免疫沉淀。截断的12kd翻译产物可能在密码子247处启动，两种抗血清都不能识别。SDS-PAGE观察到另一种表观分子量为46 kD的免疫可沉淀蛋白，该蛋白被解释为D-2多肽的同质聚集体。当添加甲醇溶解2,6二氯对苯醌或其他醌时，可以观察到全长和截短的D-2多肽的优先翻译。讨论了体外合成的D-2多肽在结合醌类、其他电子载体和叶绿素发色团研究中的应用。
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引用次数: 3
Purification and amino acid sequence determination of an endo-1,3-β-glucanase from barley 大麦内切-1,3-β-葡聚糖酶的纯化及氨基酸序列测定
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02904468
G. M. Ballance, I. Svendsen
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引用次数: 28
Chromosome pairing and synaptonemal complex formation in wheat-rye hybrids 小麦-黑麦杂交种的染色体配对和突触复合体形成
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02907178
Xingzhi Wang, P. Holm
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引用次数: 19
Role of Escherichia coli beta-ketoacyl-ACP synthase I in unsaturated fatty acid synthesis. 大肠杆菌β -酮酰基- acp合成酶I在不饱和脂肪酸合成中的作用。
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02983312
M Siggaard-Andersen

Two activities were found in E. coli extracts which could complement unsaturated fatty acid synthesis of a cerulenin treated E. coli fatty acid synthetase. One of these is beta-ketoacyl-ACP synthase I, but it is not known whether the other activity represents the previously characterized beta-ketoacyl-ACP synthase II. A mutant strain exhibiting a temperature sensitive unsaturated fatty acid synthetic activity apparently lacked an active beta-ketoacyl-ACP synthase I.

在大肠杆菌提取物中发现有两种活性可以补充蓝绿蛋白处理的大肠杆菌脂肪酸合成酶的不饱和脂肪酸合成。其中之一是β -酮酰基- acp合成酶I，但不知道其他活性是否代表先前表征的β -酮酰基- acp合成酶II。表现出温度敏感的不饱和脂肪酸合成活性的突变菌株显然缺乏活性的β -酮酰基- acp合成酶I。
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引用次数: 8
Low temperature absorption spectroscopy of barley mutants. Guassian deconvolution and fourth derivative analysis 大麦突变体的低温吸收光谱。高斯反卷积和四阶导数分析
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02983310
D. Simpson
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引用次数: 8
Chromosome pairing and synaptonemal complex formation in hexaploid wheat, monoisosomic and diisosomic for the long arm of chromosome 5B 小麦六倍体染色体配对和突触复合体的形成，染色体5B长臂的单同工体和二同工体
Carlsberg Research Communications
Pub Date : 1988-01-01 DOI: 10.1007/BF02907176
P. Holm
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引用次数: 13
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