Pub Date : 2026-02-26DOI: 10.1007/s00018-026-06107-2
Chiara Maria Calvanese, Vincenzo Valentino, Annachiara De Prisco, Serena Allesina, Angela Amoruso, Francesca Deidda, Annalisa Visciglia, Danilo Ercolini, Marco Pane, Francesca De Filippis
{"title":"Women's health is a team effort: probiogenomics supports the development of a multi-species vaginal probiotic.","authors":"Chiara Maria Calvanese, Vincenzo Valentino, Annachiara De Prisco, Serena Allesina, Angela Amoruso, Francesca Deidda, Annalisa Visciglia, Danilo Ercolini, Marco Pane, Francesca De Filippis","doi":"10.1007/s00018-026-06107-2","DOIUrl":"10.1007/s00018-026-06107-2","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12957687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147288927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-26DOI: 10.1007/s00018-026-06079-3
Pauline Stephan, Damien Plassard, William M Keyes, Yann Hérault
{"title":"Cdkn2a/p16Ink4a loss impairs Spatial memory independently of Alzheimer's-associated genetic pathways in young adult mice.","authors":"Pauline Stephan, Damien Plassard, William M Keyes, Yann Hérault","doi":"10.1007/s00018-026-06079-3","DOIUrl":"10.1007/s00018-026-06079-3","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12957686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147302987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-26DOI: 10.1007/s00018-025-06053-5
Irem Kural, Lobke Marie M Mombeek, David M Wilson
{"title":"Role of mitochondria in neuronal function and survival in the enteric and central nervous systems.","authors":"Irem Kural, Lobke Marie M Mombeek, David M Wilson","doi":"10.1007/s00018-025-06053-5","DOIUrl":"10.1007/s00018-025-06053-5","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12953807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147288850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-25DOI: 10.1007/s00018-026-06146-9
Xingtian Kong, Junwen Liu, Qiyun Zhu, Shuai Xu
{"title":"Enolase 1 suppresses influenza A virus replication by blocking the nuclear import of the viral ribonucleoprotein complex.","authors":"Xingtian Kong, Junwen Liu, Qiyun Zhu, Shuai Xu","doi":"10.1007/s00018-026-06146-9","DOIUrl":"10.1007/s00018-026-06146-9","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12953822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-25DOI: 10.1007/s00018-026-06143-y
Kameliya Roegner, Vera A Kulow, Ralf Mrowka, Kristina Engel, Bayram Edemir, Mumtaz Kasim, Cem Erdogan, Laetitia Malotka, Michael Fähling, Robert Labes
{"title":"BPGM shapes NFAT5-driven cellular responses.","authors":"Kameliya Roegner, Vera A Kulow, Ralf Mrowka, Kristina Engel, Bayram Edemir, Mumtaz Kasim, Cem Erdogan, Laetitia Malotka, Michael Fähling, Robert Labes","doi":"10.1007/s00018-026-06143-y","DOIUrl":"10.1007/s00018-026-06143-y","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12960884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147302996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-21DOI: 10.1007/s00018-026-06126-z
Miraj Ud Din Momand, Petra Majerova, Diana Mjartinova, Natalia Maruskinova, Karolina Albertusova, Michael Dobrota, Lubica Fialova, Sara Stefankova, Petar Podlesniy, Muhammad Khalid Muhammadi, Miroslav Balaz, Dominika Fricova
Alpha‑synuclein (α‑syn) overexpression models are widely used to unravel the molecular mechanisms of Parkinson's disease (PD), particularly in light of the dose-dependent transition between its physiological and toxic roles. However, existing systems rely on inducible expression, lack robust dose stratification and comparative cellular contexts. Here, we developed and characterized a panel of stable neuronal cell lines in two human cellular models (SH‑SY5Y neuroblastoma cells and ReNcell VM neural progenitors) overexpressing GFP-tagged wild-type (WT) or A53T mutant α‑syn at low and high overexpression levels. Utilizing this framework, we demonstrated that A53T consistently induces cytotoxicity, oxidative stress and mitochondrial dysfunction in both cell types. In contrast, WT α‑syn had divergent effects depending on the cellular context. In SH‑SY5Y cells, it enhanced mitochondrial function and viability, whereas in ReNcell VM cells, the same protein triggered mitochondrial impairment and elevated oxidative stress. This opposing metabolic response was reflected in increased respiratory activity in SH‑SY5Y cells and a marked decline across WT α‑syn overexpressing ReNcell VM. Importantly, post-translational modification (PTM) landscape of overexpressed WT α‑syn varied dramatically by cell type. ReNcell VM cells exhibited more robust modifications signatures, even in the absence of overt aggregation, which highlights a cell-type-specific PTM landscape that may contribute to differential vulnerability. Our findings underscore a complex interplay between α‑syn dosage, mutational status, cellular environment, and PTM profiles highlighting that neuronal vulnerability in PD is context-dependent. This work establishes a modular in vitro platform for dissecting α‑syn pathology and testing targeted therapeutic strategies grounded in cell-type specificity.
{"title":"Alpha-synuclein overexpression triggers divergent cellular responses and post-translational modifications in SH-SY5Y and ReNcell VM models.","authors":"Miraj Ud Din Momand, Petra Majerova, Diana Mjartinova, Natalia Maruskinova, Karolina Albertusova, Michael Dobrota, Lubica Fialova, Sara Stefankova, Petar Podlesniy, Muhammad Khalid Muhammadi, Miroslav Balaz, Dominika Fricova","doi":"10.1007/s00018-026-06126-z","DOIUrl":"10.1007/s00018-026-06126-z","url":null,"abstract":"<p><p>Alpha‑synuclein (α‑syn) overexpression models are widely used to unravel the molecular mechanisms of Parkinson's disease (PD), particularly in light of the dose-dependent transition between its physiological and toxic roles. However, existing systems rely on inducible expression, lack robust dose stratification and comparative cellular contexts. Here, we developed and characterized a panel of stable neuronal cell lines in two human cellular models (SH‑SY5Y neuroblastoma cells and ReNcell VM neural progenitors) overexpressing GFP-tagged wild-type (WT) or A53T mutant α‑syn at low and high overexpression levels. Utilizing this framework, we demonstrated that A53T consistently induces cytotoxicity, oxidative stress and mitochondrial dysfunction in both cell types. In contrast, WT α‑syn had divergent effects depending on the cellular context. In SH‑SY5Y cells, it enhanced mitochondrial function and viability, whereas in ReNcell VM cells, the same protein triggered mitochondrial impairment and elevated oxidative stress. This opposing metabolic response was reflected in increased respiratory activity in SH‑SY5Y cells and a marked decline across WT α‑syn overexpressing ReNcell VM. Importantly, post-translational modification (PTM) landscape of overexpressed WT α‑syn varied dramatically by cell type. ReNcell VM cells exhibited more robust modifications signatures, even in the absence of overt aggregation, which highlights a cell-type-specific PTM landscape that may contribute to differential vulnerability. Our findings underscore a complex interplay between α‑syn dosage, mutational status, cellular environment, and PTM profiles highlighting that neuronal vulnerability in PD is context-dependent. This work establishes a modular in vitro platform for dissecting α‑syn pathology and testing targeted therapeutic strategies grounded in cell-type specificity.</p>","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RFFL-mediated protein quality control limits functional rescue of TRID-CFTR modulator combination therapy for cystic fibrosis nonsense mutations.","authors":"Hazuki Tateishi, Yukako Doi, Yuka Kamada, Tsukasa Okiyoneda","doi":"10.1007/s00018-026-06114-3","DOIUrl":"10.1007/s00018-026-06114-3","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-19DOI: 10.1007/s00018-026-06080-w
Swarnendu Mukhopadhyay, Qi Gao, Tapas K Manna, Elmar Schiebel
{"title":"The γ-TuRC: mastermind of multipurpose actions at the centrosome.","authors":"Swarnendu Mukhopadhyay, Qi Gao, Tapas K Manna, Elmar Schiebel","doi":"10.1007/s00018-026-06080-w","DOIUrl":"10.1007/s00018-026-06080-w","url":null,"abstract":"","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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