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Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides最新文献

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Incorporation of radioactive glucosamine into submicrosomal fractions isolated from rat liver 从大鼠肝脏分离的亚微粒体中掺入放射性氨基葡萄糖
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90035-7
L. Helgeland

The incorporation of [1-14C[glucosamine into various submicrosomal fractions and cell sap from rat liver, as well as into plasmaproteins, has been examined after intraperitoneal injection. It has been shown that after 1 h there is a significant incorporation of protein-bound radioactivity into the submicrosomal fractions. 69% of the total radioactivity was found in the ultrasonic extract of the microsomal pellet, 28% in the subsequent deoxycholate extract and 3% associated with the ribosomes. Of the total radioactivity in the ultrasonic extract and the deoxycholate extract, more than 80% was found in the perchloric acid precipitate and only about 5–10% in the phosphotungstic acid precipitate. 15% of the protein-bound radioactivity in the perchloric acid precipitate of the ultrasonic extract was associated with a sialic acid fraction and about 1% found in the hexose fraction. Most of the remaining radioactivity was associated with glucosamine. The intracellular site for the biosynthesis of glycoproteins is discussed.

经腹腔注射后，研究了[1-14C]葡萄糖胺与大鼠肝脏的各种亚微粒体组分和细胞液以及血浆蛋白的结合情况。研究表明，在1小时后，亚微粒体中存在显著的蛋白质结合放射性。总放射性的69%发现于微粒体颗粒的超声提取物中，28%发现于随后的脱氧胆酸提取物中，3%与核糖体有关。超声提取物和脱氧胆酸提取物的总放射性中，高氯酸沉淀物的放射性含量超过80%，磷钨酸沉淀物的放射性含量仅为5-10%。超声波提取物的高氯酸沉淀物中15%的蛋白质结合放射性与唾液酸部分有关，约1%在己糖部分中发现。剩余的大部分放射性物质与氨基葡萄糖有关。讨论了糖蛋白生物合成的细胞内位点。
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引用次数: 29
A glucose-containing glycopeptide from bovine aorta glycoprotein 从牛主动脉糖蛋白中提取的含葡萄糖的糖肽
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90040-0
B. Radhakrishnamurthy, A.F. Fishkin, G.S. Berenson
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引用次数: 8
Isolation of new polysaccharide sulphates from charonia lampas 新硫酸盐多糖的分离
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90026-6
Sadako Inoue

A sulphated heteropolysaccharide named horatinsulphuric acid has been isolated from a sulphatase-rich gastropod, Charonia lampas. Horatinsulphuric acid, a mixture of analogous polymers, is obtained by the method of trypaflavin precipitation of sulphate esters. Fractionation made by column chromatography using DEAE-Sephadex separates the mixture into several fractions in which the sulphate content varies from 10 to 30%. The composition of the sugar portion consists of fucose, mannose, glucose, galactose, glucosarnine, galactosamine and sialic acid.

从一种富含硫酸盐酶的腹足动物Charonia lampas中分离出一种硫酸异多糖，命名为horatinsulphuric acid。Horatinsulphuric acid horatinsulphacid Horatinsulphuric acid Horatinsulphuric acid horatinsulphacid是一种类似聚合物的混合物，可以通过胰黄素沉淀硫酸盐酯的方法得到。采用DEAE-Sephadex柱层析进行分馏，将混合物分成几个馏分，其中硫酸盐含量从10%到30%不等。所述糖部分的组成包括焦糖、甘露糖、葡萄糖、半乳糖、葡萄糖氨酸、半乳糖胺和唾液酸。
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引用次数: 26
Extent of cross linkage in the murein sacculus∗ of Escherichia coli B cell wall 大肠杆菌B细胞壁小鼠小囊*的交联程度
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90038-2
I. Takebe
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引用次数: 48
Effect of prednisolone and hydrocortisone on distribution of mucopolysaccharides in the skin of rats 强的松和氢化可的松对大鼠皮肤粘多糖分布的影响
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90042-4
Sara Schiller , Nellie Blumenkrantz, Albert Dorfman
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引用次数: 19
The chemistry of the cell walls of Bacillus anthracis: the effect of penicillin 炭疽芽孢杆菌细胞壁的化学性质:青霉素的作用
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90024-2
Ronald S. Ratney

  • 1.

    1. Cell wall mucopeptide of Bacillus anthracis, strain V1B-189 was prepared from cells grown in the presence and absence of penicillin. Both types contain an unusual inner membrane.

  • 2.

    2. These were analysed for reducing sugars, amino sugars and amino acids. Both types contained glutamic acid, alanine and diaminopimelic acid as the principle amino acids in the mole ratios of 1:1.5:1.

  • 3.

    3. Penicillin did not change the relative amounts of the different cell wall constituents but it did reduce the weight of all constituents per unit weight of mucopeptide.

1.1. 以有无青霉素条件下生长的炭疽芽孢杆菌V1B-189细胞壁粘肽为原料制备炭疽芽孢杆菌细胞壁粘肽。这两种类型都含有一种不寻常的内膜。对它们进行了还原糖、氨基糖和氨基酸的分析。两种类型均以1:1.5:1.3.3摩尔比的谷氨酸、丙氨酸和二氨基戊酸为主要氨基酸。青霉素没有改变不同细胞壁成分的相对量，但降低了单位重量黏肽中所有成分的重量。
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引用次数: 9
Conformation of the iduronic acid moiety of chondroitin sulfate B (Dermatan sulfate) 硫酸软骨素B (Dermatan sulfate)中伊杜醛酸部分的构象
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90037-0
E.A. Davidson
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引用次数: 20
On the metabolic role of β-glucuronidase β-葡萄糖醛酸酶的代谢作用
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90043-6
J. Mora, L. Can̄edo, G. Soberön
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引用次数: 10
Investigations on the chemistry of heparin V. Disaccharides obtained after partial hydrolysis 肝素V.部分水解后二糖的化学性质研究
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90028-X
I. Danishefsky, Harvey Steiner

  • 1.

    1. The structure of the polysaccharide backbone of heparin was investigated by means of graded hydrolysis procedures.

  • 2.

    2. Desulfated acetamido heparin was partially hydrolyzed with acid and the products were fractionated on a cation exchange resin. This yielded glucosamine, disaccharides and a mixture of oligosaccharides.

  • 3.

    3. Two disaccharides were isolated as their acetamido derivatives. One of these was identified as 4-O-(2-acetamido-2-deoxy-α-d-glucopyranoside)-d-glucopyranosyl-uronic acid and the other as 4-O-(d-glucopyranosyluronic acid)-2-acetamido-2-deoxy-d-glucopyranose.

  • 4.

    4. These results indicate that heparin contains alternating 1,4-linked units of glucosamine and glucuronic acid.

1.1. 采用分级水解法对肝素多糖骨架结构进行了研究。用酸对脱硫后的乙酰氨基肝素进行部分水解，产物在阳离子交换树脂上进行分馏。这就产生了葡萄糖胺、双糖和低聚糖的混合物。分离得到两个双糖作为它们的乙酰氨基衍生物。其中一个鉴定为4-O-(2-乙酰氨基-2-脱氧-α-d-葡萄糖吡喃苷)-d-葡萄糖吡喃醛酸，另一个鉴定为4-O-(d-葡萄糖吡喃醛酸)-2-乙酰氨基-2-脱氧-d-葡萄糖吡喃糖。这些结果表明肝素含有葡萄糖胺和葡萄糖醛酸交替的1,4-连接单元。
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引用次数: 55
The synthesis of teichoic acid IV. On the regulation of cytidine 5′-diphosphateglycerol concentration 磷壁酸的合成。胞苷5′-二磷酸甘油酯浓度的调控
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90025-4
Luis Glaser

The partial purification of a cytidine 5′-diphosphateglycerol pyrophosphatase from Bacillus subtilis (NCTC 3610) is described. The Km for cytidine 5′-diphosphateglycerol is 1.6·10−3M. It is suggested that the function of this enzyme is to prevent the accumulation of cytidine 5′-diphosphateglycerol under conditions where the rate of teichoic acid synthesis is decreased. A similar mechanism is suggested for the control in vivo of the concentration of some other nucleoside diphosphate sugars.

报道了枯草芽孢杆菌(NCTC 3610)胞苷5′-二磷酸甘油三酯焦磷酸酶的部分纯化。胞苷5′-二磷酸甘油酯的Km为1.6·10−3M。这表明该酶的功能是在胆酸合成速率降低的条件下防止胞苷5′-二磷酸甘油三酯的积累。类似的机制也适用于体内控制其他核苷二磷酸糖的浓度。
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引用次数: 19
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Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides
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