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Microwave ‘dry’ distillation as an useful tool for extraction of edible essential oils 微波“干”蒸馏作为一种有用的工具提取食用精油
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.09.007
N. Tigrine-Kordjani , B.Y. Meklati , F. Chemat

Microwave “Dry” Distillation or microwave accelerated distillation (MAD) is proposed as a method for “green” extraction of edible essential oils extensively used in the fragrance, flavour, and pharmaceutical industries and also in aromatherapy. It is a combination of microwave heating and dry distillation, performed at atmospheric pressure without adding any solvent or water. Isolation and concentration of volatile compounds is performed by a single stage. Rosemary was extracted with MAD at atmospheric pressure and 100 °C for 30 min. The extracted compounds were removed from the aqueous extract by simple decantation, determined by gas chromatography-flame ionisation (GC-FID) and identified by gas chromatography–mass spectrometry (GC/MS). Hydrodistillation of rosemary was performed with 2 L of water for 3 h for comparison of the results with those provided by the proposed method: extraction time, yields, chemical composition and quality of the essential oil, efficiency and costs of the process. Extraction of essential oils from rosemary with MAD was better in terms of energy saving, extraction time (30 min versus 3 h), oxygenated fraction (59% versus 46%), exact product yield (0.6% versus 0.6%) and product quality. MAD is a green technology and appears as a good alternative for the extraction of edible essential oils from aromatic plants used in aromatherapy and the food industry.

微波“干”蒸馏或微波加速蒸馏(MAD)被提出作为一种“绿色”提取食用精油的方法,广泛应用于香料、香精、制药工业和芳香疗法。它是微波加热和干馏的结合,在常压下进行,不添加任何溶剂或水。挥发性化合物的分离和浓缩由一个阶段完成。在常压、100℃条件下,用MAD提取迷迭香30 min。通过简单滗析将提取的化合物从水提物中分离出来,通过气相色谱-火焰离子化(GC- fid)测定,并通过气相色谱-质谱(GC/MS)鉴定。用2升水对迷迭香进行了3小时的加氢蒸馏,并将结果与所提出的方法进行了比较:提取时间、得率、精油的化学成分和质量、工艺效率和成本。在节能、提取时间(30 min vs . 3 h)、氧化分数(59% vs . 46%)、产品收率(0.6% vs . 0.6%)和产品质量方面,MAD提取迷迭香精油效果更好。MAD是一项绿色技术,是芳香疗法和食品工业中从芳香植物中提取食用精油的一个很好的选择。
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引用次数: 43
Which essential oil is better for hygienic massage practice? 哪种精油更适合卫生按摩?
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2005.10.006
N. Donoyama, Y. Ichiman

We examined whether it was possible to practice hygienic massage by using six essential oils (eucalyptus, lavender, niaouli, sage, tea tree, and thyme linalool) that in previous studies or anecdotally have been found to have antibacterial effects. First, to determine the inhibitory properties of the six essential oils against 4.80 × 105 colony forming units (CFU) of strain ATCC-25923 of Staphylococcus aureus, we used a disc method to measure the inhibition zones. Niaouli and eucalyptus showed higher growth inhibitory effects. We then examined the results of using these two essential oils in seven different massage sessions. The niaouli and eucalyptus were each diluted to 1%, 3%, or 6% v/v with jojoba oil base and jojoba oil without any essential oil was used as a control. Bacterial samples were taken from the therapist’s palms and the subject’s skin, and the surviving bacteria were counted.

The antibacterial effects were correlated in vitro with the concentration of the essential oil and massage sessions with niaouli oil were more hygienic than those with eucalyptus oil.

我们研究了是否有可能通过使用六种精油(桉树、薰衣草、牛油、鼠尾草、茶树和百里香芳樟醇)来进行卫生按摩,这些精油在以前的研究中或传闻中被发现具有抗菌作用。首先,为了确定6种精油对金黄色葡萄球菌ATCC-25923菌株4.80 × 105菌落形成单位(CFU)的抑制性能,我们采用圆盘法测定了抑制区。牛梨和桉树表现出较强的生长抑制作用。然后,我们检查了在七个不同的按摩过程中使用这两种精油的结果。将牛油和桉树分别用荷荷巴油基稀释至1%、3%或6% v/v,以不含任何精油的荷荷巴油为对照。从治疗师的手掌和受试者的皮肤上提取细菌样本,并对存活的细菌进行计数。体外抑菌效果与精油浓度相关,用牛油按摩比用桉树油按摩更卫生。
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引用次数: 5
Research reports 研究报告
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.09.004
Bob Harris

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引用次数: 0
In vitro activity of essential oils on microorganisms isolated from vaginal infections 精油对阴道感染分离微生物的体外活性研究
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.09.005
A. Schwiertz , C. Duttke , J. Hild , H.J. Müller

The antibacterial and antifungal activity of ten essential oils was tested against a range of vaginal bacterial and fungal strains isolated from existing vaginal infections including Atopobium vaginae, Gardnerella vaginalis, Bacteroides vulgatus, Streptococcus agalactiae, H2O2-producing lactobacilli and non H2O2-producing lactobacilli, Candida albicans, Candida glabrata, Candida parapsilosis and Candida tropicalis.

Investigation of the in vitro susceptibility of the essential oils against bacteria revealed that lemongrass, tea tree and lavender exhibited the lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at 1–2.5 μl/ml, thus being the most potent essential oils against the tested bacteria. Interestingly, the MIC and MBC values of palmarosa, neroli, manuka, rose-scented geranium, rosemary, common thyme and clary sage were at ⩾7.5 μl/ml for protective H2O2-lactobacilli but lower for pathogentic bacteria. The MIC and minimal fungicidal concentration (MFC) for lemongrass, palmarosa, lavender and rose scented geranium were at 1–2.5 μl/ml. All other essential oils were less potent.

Overall, lemongrass, palmarosa, lavender and rose scented geranium were the most potent oils in the inhibition of pathogenic bacteria and fungi. However, further investigations in the form of clinical studies are necessary to ascertain the therapeutic use of these essential oils in the combat of vaginal infections.

研究了10种精油对从现有阴道感染中分离出的阴道细菌和真菌菌株的抗菌和抗真菌活性,这些细菌和真菌菌株包括阴道阿托波菌、阴道加德纳菌、普通拟杆菌、无乳链球菌、产生h2o2的乳酸菌和不产生h2o2的乳酸菌、白色念珠菌、光秃念珠菌、假丝酵母菌和热带念珠菌。结果表明,香茅精油、茶树精油和薰衣草精油的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)均为1 ~ 2.5 μl/ml,是抑菌效果最好的精油。有趣的是,棕榈草、橙花、麦卢卡、玫瑰香天竺葵、迷迭香、普通百里香和鼠尾草的MIC和MBC值对于保护性h2o2 -乳酸菌为小于或等于7.5 μl/ml,但对于致病菌为小于或等于7.5 μl/ml。柠檬草、棕榈草、薰衣草和玫瑰天竺葵的MIC和最小杀真菌浓度(MFC)为1 ~ 2.5 μl/ml。其他所有的精油都没有那么有效。总体而言,柠檬草、棕榈草、薰衣草和玫瑰天竺葵精油对病原菌和真菌的抑制作用最强。然而,需要进一步的临床研究来确定这些精油在对抗阴道感染中的治疗用途。
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引用次数: 36
To what extent does aromatherapy use in palliative cancer care improve quality of life and reduce levels of psychological distress? A literature review 在姑息性癌症治疗中使用芳香疗法在多大程度上改善了生活质量并减少了心理困扰水平?文献综述
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.01.003
L.J. Bowers

Public and healthcare professionals’ interest in the use of aromatherapy to ease levels of psychological distress and improve the quality of life for cancer patients is greater than ever. Aromatherapy is increasingly incorporated into clinical practice, particularly in palliative care settings, and has been reported as the most commonly used complementary therapy within the NHS. Yet questions remain regarding the safety and efficacy of essential oil use and there are concerns around the evidence available to support actual benefits. Much of the evidence is anecdotal with a distinct lack of quantitative and objective data available, due to concerns about scientific research methods for complementary therapies. This article is a review of the literature relating to the safety and efficacy of aromatherapy use for cancer care, and will highlight methodological issues and implications for research. However, the primary aim is to provide cancer patients and their carers’ access to the body of information and research available, thereby enabling patient empowerment through choice and education.

公众和医疗保健专业人员对使用芳香疗法来缓解癌症患者的心理困扰和改善生活质量的兴趣比以往任何时候都大。芳香疗法越来越多地被纳入临床实践,特别是在姑息治疗环境中,据报道,芳香疗法是NHS中最常用的补充疗法。然而,关于使用精油的安全性和有效性的问题仍然存在,人们对支持实际益处的现有证据感到担忧。由于对补充疗法的科学研究方法的担忧,许多证据都是轶事,明显缺乏定量和客观的数据。这篇文章是关于芳香疗法用于癌症治疗的安全性和有效性的文献综述,并将强调方法学问题和研究的意义。然而,主要目的是为癌症患者及其护理人员提供获得现有信息和研究的途径,从而使患者能够通过选择和教育获得权力。
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引用次数: 9
Identification of the volatile constituents of Brachystegia eurycoma Harms 短肌肌肉瘤挥发性成分的鉴定
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.09.002
A.O. Ogunbinu , I.A. Ogunwande , T.M. Walker , W.N. Setzer

The volatile oil obtained by hydrodistillation of air-dried samples of the leaf of Brachystegia eurycoma Harms, Caesalphinaceae, have been studied for their constituents by GC–MS. Oxygenated monoterpenoids (35.9%) and sesquiterpenoid hydrocarbons (30.7%) are the major class of compounds identified occurring in higher proportions. The main compounds of the leaf oil are 1,8-cineole (23.1%) and acorenone (10.0%). β-Caryophyllene (5.6%) and geranyl acetone (4.5%) are also present in significant amounts. This is first report on the constituents of the oil of the plants in the genus Brachystegia. The potential chemotherapeutic effects of the oil are discussed.

采用气相色谱-质谱联用技术,对经风干的Brachystegia eurycoma Harms, Caesalphinaceae叶子的水蒸气蒸馏法得到的挥发油进行了成分分析。含氧单萜类化合物(35.9%)和倍半萜类化合物(30.7%)是发现的比例较高的化合物的主要类别。叶油的主要成分为1,8-桉叶油脑(23.1%)和香芹酮(10.0%)。β-石竹烯(5.6%)和香叶丙酮(4.5%)也大量存在。本文首次报道了腕足属植物的油脂成分。并对其潜在的化疗作用进行了讨论。
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引用次数: 7
Investigations on the essential oil of Cymbopogon giganteus from Benin for its potential use as an anti-inflammatory agent 贝宁巨足香蒲精油抗炎作用的研究
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.01.001
G.A. Alitonou , F. Avlessi , D.K. Sohounhloue , H. Agnaniet , J.-M. Bessiere , C. Menut

The species of Cymbopogon giganteus is widely used in traditional medicine against several diseases. This study reports the inhibitory effect produced by the chemical constituents of the essential oil from leaves of C. giganteus of Benin, in vitro on 5-lipoxygenase. We assayed the antiradical scavenging activity of the sample by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method.

The essential oil of C. giganteus was analyzed by GC and GC/MS. The major constituents were: trans-p-1(7),8-menthadien-2-ol (22.3%), cis-p-1(7),8-menthadien-2-ol (19.9%), trans-p-2,8-menthadien-1-ol (14.3%), cis-p-2,8-menthadien-1-ol (10.1%).

Cymbopogon giganteus在传统医学中被广泛用于治疗多种疾病。本研究报道了贝宁巨竹叶精油化学成分对体外5-脂氧合酶的抑制作用。采用1,1-二苯基-2-苦味酰肼(DPPH)法测定样品的抗自由基清除活性。采用气相色谱(GC)和质谱(GC/MS)分析了香茅挥发油的成分。主要成分为:反式-p-1(7)、8-薄荷二烯-2-醇(22.3%)、顺式-p-1(7)、8-薄荷二烯-2-醇(19.9%)、反式-p-2,8-薄荷二烯-1-醇(14.3%)、顺式-p-2,8-薄荷二烯-1-醇(10.1%)。
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引用次数: 62
Antibacterial activity of essential oils, hydrosols and plant extracts from Australian grown Lavandula spp. 澳洲薰衣草精油、水溶胶及植物提取物的抗菌活性。
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.01.007
T. Moon, J.M. Wilkinson, H.M.A. Cavanagh

Although there is considerable anecdotal information about the antibacterial activity of lavender oils, much of this has not been substantiated by scientific or clinical evidence. In this study we assessed the activity of lavender essential oils, hydrosols and aqueous and ethanolic foliage extracts from a range of Australian grown Lavandula species. The results support the anecdotal use of lavender oils as antibacterial agents and demonstrated that some oils which had previously not been investigated (e.g., Lavandula heterophylla) display good antibacterial activity against a range of bacteria including Streptococcus pyogenes, Staphylococcus aureus, MRSA, Citrobacter freundii, Proteus vulgaris, Escherichia coli, VRE and Propionibacterium acnes. Pseudomonas aeruginosa was the only bacterium not susceptible to any essential oil. There was considerable variability in the activity of the essential oils however; no one oil produced the highest level of antibacterial activity against all bacteria. No correlation was observed between the percentage of major chemical components and antibacterial activity. The lavender hydrosols and aqueous foliage extracts did not have any antibacterial activity. Six of the ethanolic extracts displayed activity against Pr. vulgaris but no activity against any other organism. Further work is required to determine whether these in vitro results will be realised in a clinical environment but it is clear that not all lavenders are equal in terms of their antibacterial properties.

虽然关于薰衣草精油的抗菌活性有相当多的轶事信息,但其中大部分都没有得到科学或临床证据的证实。在这项研究中,我们评估了薰衣草精油,水溶胶和水乙醇叶提取物的活性从一系列澳大利亚种植的薰衣草物种。研究结果支持了将薰衣草精油作为抗菌剂的传闻,并表明一些以前未被研究过的精油(如薰衣草)对一系列细菌具有良好的抗菌活性,包括化脓性链球菌、金黄色葡萄球菌、MRSA、弗氏柠檬酸杆菌、寻常变形杆菌、大肠杆菌、VRE和痤疮丙酸杆菌。铜绿假单胞菌是唯一对精油不敏感的细菌。然而,精油的活性有相当大的可变性;没有一种油对所有细菌的抗菌活性都是最高的。主要化学成分的含量与抗菌活性无相关性。薰衣草水溶胶和叶片水提液均无抗菌活性。其中6种乙醇提取物对寻常病菌有抑制作用,但对其他生物无抑制作用。需要进一步的工作来确定这些体外结果是否会在临床环境中实现,但很明显,并非所有薰衣草在抗菌性能方面都是相同的。
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引用次数: 69
Studies on the essential oil composition and antimicrobial activity of Thymus algeriensis Boiss. et Reut 阿尔及利亚胸腺精油成分及抑菌活性研究。et Reut
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.04.003
T. Dob , D. Dahmane , T. Benabdelkader , C. Chelghoum

Many species of Thymus have been widely used in Algerian folk medicine as astringent, expectorant and cicatrising agents. This study was designed to investigate for the first time the essential oil of the aerial parts of wild growing Thymus algeriensis endemic in North Africa. The volatile oil obtained by hydrodistillation was characterized by the physico-chemical properties, GC and GC/MS techniques. The yield of the oil was 1.13% (w/w), based on dry weight. Fifty-five components, representing 94.3% of the total oil, were identified. The oil was distinguished by its high content of oxygenated monoterpenes (79.5%) and was found to possess the following major components: linalool (47.3%), thymol (29.2%) and p-cymene (6.8%). Furthermore, the oil was tested for antimicrobial activity against four bacteria, two fungi and two yeasts. This oil exhibited a significant in vitro antimicrobial activity against Bacillus subtilis (MIC = 0.5 μL/mL), as well as against all yeast and all filamentous fungi tested (MIC = 0.5 and 1.0 μL/mL).

许多种类的胸腺草在阿尔及利亚民间医学中被广泛用作收敛、祛痰和愈合剂。本研究首次对北非特有野生阿尔及利亚胸腺(thyymus algeriensis)地上部分的挥发油进行了研究。采用气相色谱(GC)和质谱(GC/MS)技术对加氢蒸馏得到的挥发油进行了理化性质表征。以干重为基准,油得率为1.13% (w/w)。鉴定出55种组分,占总油的94.3%。其主要成分为芳樟醇(47.3%)、百里香酚(29.2%)和对伞花烃(6.8%)。此外,还测试了该油对四种细菌、两种真菌和两种酵母的抗菌活性。该精油对枯草芽孢杆菌(MIC = 0.5 μL/mL)、酵母菌和丝状真菌(MIC = 0.5和1.0 μL/mL)均有显著的抑菌活性。
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引用次数: 96
For a deeper understanding of tea tree oil: Fresh is best – why we should only use fresh oil at any concentration 为了更深入地了解茶树油:新鲜的是最好的-为什么我们应该只使用新鲜的油在任何浓度
Pub Date : 2006-01-01 DOI: 10.1016/j.ijat.2006.09.009
D. Wabner, K. Geier, D. Hauck

We should remember the widespread beneficial and practical uses of tea tree oil in aromatherapy and cosmetics worldwide. Tea tree oil is now under regulatory threat from the European Union. In the following article some important arguments are raised that reveal other sides to important aspects of this discussion. The results of two PhD theses, a comprehensive literature study report and a survey about the use of the oil could help to avoid a total and unwarranted prohibition of tea tree oil.

我们应该记住茶树油在芳香疗法和化妆品中的广泛有益和实际用途。茶树油现在受到欧盟监管的威胁。在下面的文章中,提出了一些重要的论点,揭示了讨论的其他方面。两篇博士论文的结果,一份全面的文献研究报告和一项关于茶树油使用的调查可以帮助避免全面和无端地禁止茶树油。
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引用次数: 10
期刊
International Journal of Aromatherapy
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