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Evaluating the capabilities of artificial intelligence for interpretation and retrieval of research data stored in a repository 评估人工智能解释和检索存储在存储库中的研究数据的能力
Pub Date : 2025-05-08 DOI: 10.1002/its2.70046
Mariah K. Cashbaugh, Paul L. Koch, Gerald L. Miller

The current format of data repositories like Plant Disease Management Reports (PDMRs) makes recovery of critical information such as treatment efficacy and pathogen control difficult. Artificial intelligence was employed to retrieve and evaluate data from the subset of reports related to turfgrass disease management. Artificial intelligence has the potential for application across a broad spectrum of report types and could enhance the efficiency of translating research data into management recommendations. A customized large language model with access to 1074 PDF files containing turfgrass PDMRs was asked a series of queries. The answers were recorded, and the accuracy of the given responses was verified. From these responses, we can conclude that while the queries were often answered in a thorough manner as specified in the chatbot's configuration, the accuracy and consistency of responses given was highly variable and dependent on which PDMRs the chatbot deemed relevant to the query. Further modifications and validation of chatbots would be required before effective utilization as an extension and research tool.

植物病害管理报告(PDMRs)等数据存储库的当前格式使得诸如治疗效果和病原体控制等关键信息的恢复变得困难。人工智能用于检索和评估与草坪草疾病管理相关的报告子集中的数据。人工智能具有广泛应用于报告类型的潜力,可以提高将研究数据转化为管理建议的效率。一个定制的大型语言模型可以访问包含草坪草PDMRs的1074个PDF文件,并进行了一系列查询。他们的回答被记录下来,并验证了所给出回答的准确性。从这些响应中,我们可以得出结论,虽然查询通常以聊天机器人配置中指定的彻底方式回答,但给出的响应的准确性和一致性是高度可变的,并且取决于聊天机器人认为与查询相关的PDMRs。在作为一种扩展和研究工具得到有效利用之前,需要对聊天机器人进行进一步的修改和验证。
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引用次数: 0
Diversity assessment of morphological and growth characteristics of zoysiagrass ecotypes in Japan using digital phenotyping 利用数字表型评价日本结缕草生态型形态和生长特征的多样性
Pub Date : 2025-05-07 DOI: 10.1002/its2.70056
Sorawich Pongpiyapaiboon, Sorawit Doungchoo, Hwan May Ng, Hidenori Tanaka, Ryo Akashi

Zoysiagrass (Zoysia genus) is a valuable warm-season turfgrass species that exhibits significant diversity in growth and morphological traits across different ecotypes. Traditional methods of classifying zoysiagrass ecotypes rely heavily on morphological observations. However, these methods can be labor-intensive and may not fully capture the phenotypic diversity present within the species. In this study, we evaluated the utility of non-invasive digital phenotyping to characterize zoysiagrass ecotypes. Using a digital phenotyping system (DPS) allows for precise measurements of plant height, area, 3D volume (representing biomass), and color index. Clustering algorithms were applied to assess diversity and classify zoysiagrass ecotypes. The subsequent results were compared to manual species classification and genetic marker analysis. The cluster results of DPS effectively differentiate between the three Zoysia species and demonstrate a high correspondence between digital phenotyping and traditional morphological methods. The study highlights the advantages of grouping zoysiagrass based on phenotypic traits and growth characteristics rather than solely on morphological observation or genetic markers, particularly in the context of breeding and research, where a broader range of traits provides more opportunities for selection. Future research could integrate this method with genotypic and transcriptomic analyses for a deeper understanding of zoysiagrass diversity.

结缕草(结缕草属)是一种珍贵的暖季草坪草,在不同生态型中表现出显著的生长和形态特征多样性。结缕草生态型的传统分类方法在很大程度上依赖于形态学观察。然而,这些方法可能是劳动密集型的,并且可能无法完全捕获物种内存在的表型多样性。在这项研究中,我们评估了非侵入性数字表型对结缕草生态型特征的效用。使用数字表型系统(DPS)可以精确测量植物高度,面积,3D体积(代表生物量)和颜色指数。采用聚类算法对结缕草生态型进行多样性评价和分类。随后的结果与人工物种分类和遗传标记分析进行了比较。DPS聚类结果有效地区分了三个结缕草种,并证明了数字表型与传统形态学方法之间的高度对应。该研究强调了根据结缕草的表型性状和生长特征而不是仅仅根据形态观察或遗传标记进行分组的优势,特别是在育种和研究的背景下,更广泛的性状范围为选择提供了更多的机会。未来的研究可以将该方法与基因型和转录组分析相结合,以更深入地了解结缕草的多样性。
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引用次数: 0
Evaluating rooting vigor of two zoysiagrass cultivars using a simulated sod installation protocol 利用模拟草皮安装方案评价两个结扎草品种的生根活力
Pub Date : 2025-05-07 DOI: 10.1002/its2.70057
Mohammed Kyum, Adriana Laine, Jamie Buhlman, Samantha Dorrian, Kevin Kenworthy

Sod installation rooting vigor is an important characteristic for establishment of turfgrass sod. This study evaluated rooting traits of two zoysiagrass cultivars, ‘SS-500’ (Empire®, Sod Solutions, Inc., Zoysia japonica Steud) and ‘Zeon’ (Zoysia matrella L. Merr), under controlled settings to simulate sod installation. The experiment, conducted twice, followed a randomized complete block design with a split-plot arrangement, where cultivars were the main plots and weeks of harvest were the split plots. Significant differences were observed between genotypes and harvest weeks for all root traits. Empire generally had higher values for the evaluated characteristics, and values increased over time for subsequent harvest dates. Results indicate that the methodology has merit for evaluation of sod installation rooting vigor of zoysiagrass.

草皮安装生根活力是草坪草皮建立的重要特征。本研究在模拟草皮安装的受控环境下,评估了两个结扎草品种“SS-500”(Empire®,Sod Solutions, Inc., Zoysia japonica Steud)和“Zeon”(Zoysia matrella L. Merr)的生根特性。试验进行了两次,采用完全随机区组设计,以品种为主要区,收获周数为分割区。所有根系性状在基因型和收获周之间均存在显著差异。帝国通常具有较高的评估特征值,并且随着后续收获日期的增加而增加。结果表明,该方法可用于结缕草草皮安装生根活力的评价。
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引用次数: 0
The growth of Zoysia matrella in a Japanese golf course fairway: Seven-year study 结缕草在日本高尔夫球场球道中的生长:7年研究
Pub Date : 2025-05-06 DOI: 10.1002/its2.70054
Nana Sakaguchi, Yoko Yukimura, Tetsu Cho, Shoichi Kimura

A 7-year study was conducted to investigate the seasonal change of Zoysia matrella growth in the fairway at a golf course in Kanagawa Prefecture, Japan. The turfgrass samples were collected with hole cup cutters every month from September 2016 to December 2023. The dry weight and starch content of the turfgrass shoot showed seasonal change every year, and there were differences among the years. These seasonal changes and the differences among the years are supposed to be affected by weather factors like temperature and solar irradiation. Compared to a previous report, it is suggested that the spring green-up became much earlier because of the change of temperature in these 30 years. The shoot weight and starch content during rainy season (June–July) showed correlation to the solar irradiation in the same period. The temperature showed positive correlation to starch content in November to December. Better understanding of the relationship of the turfgrass growth and the weather factors may lead to better management of Zoysia matrella in golf course fairways.

对日本神奈川县某高尔夫球场球道内结缕草生长的季节变化进行了为期7年的研究。2016年9月至2023年12月,每月使用孔杯切割器采集草坪草样本。草坪草茎部干重和淀粉含量呈季节变化,各年份间存在差异。这些季节变化和年份之间的差异应该受到温度和太阳辐照等天气因素的影响。与之前的报道相比,这30年来,由于气温的变化,春季变绿的时间提前了很多。雨季(6 ~ 7月)的茎重和淀粉含量与同期太阳辐照量呈显著相关。11 ~ 12月温度与淀粉含量呈显著正相关。更好地了解草坪草生长与天气因素的关系,可以更好地管理高尔夫球场球道中的结缕草。
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引用次数: 0
Measurement of carbon storage in schoolyard grass areas at elementary schools in Kobe, Japan 日本神户小学校园草地碳储量测量
Pub Date : 2025-05-05 DOI: 10.1002/its2.70048
Hiroyuki Kikukawa, Hideaki Watarai, Erika Eda, Bunyu Matsuda

In this study, soil samples from the lawns and bare soil of eight elementary schools that are engaged in schoolyard lawn development were collected, and the carbon sequestration amount was measured, in Kobe City, Hyogo Prefecture, Japan. Samples were taken by digging down to a depth that could be dug by hand, both for the grassland and for the bare soil for comparison. The actual sampling depth over which the carbon stock was calculated ranged from 5.0 to 15.3 cm. The samples were divided and tested for their carbon content in three layers (Thatch and Root Horizon + Humus Layer [the section of the soil core that does not contain roots] + Parent Layer) for the lawn areas. For bare soil, the sample was not divided, and the entire sample was tested for its carbon content. Additionally, the depth and weight of the sampled soil were measured. In addition to total carbon analysis using a total organic carbon meter, we also examined the elapsed years the lawn has been maintained as such and the organic status of the soil and attempted to conduct a questionnaire survey to determine any year-to-year variations. As a result of the analysis, the amount of carbon on the surface of the lawn (0–20 cm deep) was 152.9 ± 98.7 t-CO2 ha−1. The total carbon stock of the bare land was 149.5 ± 86.4 t-CO2 ha−1, and the increase in carbon stock due to the conversion of elementary school playgrounds was 3.3 ± 1.3 t-CO2 ha−1. The results of this study indicate that the conversion of elementary school playground to grasslands in Japan could contribute to carbon storage.

本研究收集了日本兵库县神户市8所从事校园草坪开发的小学草坪和裸土的土壤样品,并测量了固碳量。样本是通过挖到一个可以用手挖的深度来获取的,既可以用于草原,也可以用于裸露的土壤进行比较。计算碳储量的实际采样深度为5.0 ~ 15.3 cm。样品被分成三层(茅草和根系层+腐殖质层[不含根的土壤核心部分]+母质层),对草坪区域的碳含量进行测试。对于裸露的土壤,不将样品分开,对整个样品进行碳含量测试。此外,还测量了采样土壤的深度和重量。除了使用总有机碳计进行总碳分析外,我们还检查了草坪维持的时间和土壤的有机状态,并试图进行问卷调查以确定任何年度变化。结果表明,草坪表面(0 ~ 20 cm深)碳含量为152.9±98.7 t-CO2 ha−1。裸地总碳储量为149.5±86.4 t-CO2 ha−1,小学操场改造增加的碳储量为3.3±1.3 t-CO2 ha−1。本研究结果表明,日本小学操场改造为草地有助于碳储存。
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引用次数: 0
In vitro fungicide sensitivity of root rot disease causal agent Phialocephala bamuru isolated in New Jersey from hard fescue 新泽西硬羊茅根腐病病原菌phalocephala bamuru的体外杀菌剂敏感性研究
Pub Date : 2025-05-05 DOI: 10.1002/its2.70053
Kyle M. Genova, Chase W. Bauberger, Ming-Yi Chou

Fungal pathogen Phialochephala bamuru that causes fairway patch on warm-season turfgrasses in Australia is recently observed to cause severe root rot disease in New Jersey on cool-season turfgrass hard fescue (Festuca brevipila). Symptoms begin as small tan colored patches but can develop into irregular ring-like blighted turf upward of 3 m in diameter. Little is known on the control of this disease, with a few reports on the ineffectiveness of single and multiple active ingredient products in the field. To better identify the effective fungicides in controlling P. bamuru, 39 fungicides were evaluated in vitro for suppressing the growth of P. bamuru. Fungicides were added to a 10% potato dextrose agar media, and a P. bamuru mycelium plug was placed in the center of each plate for hyphal radial growth to be measured. Variable growth of P. bamuru was observed, indicating different fungicide sensitivities. Twelve fungicides belonging to five chemical families completely inhibited P. bamuru growth. Ten of these fungicides were then evaluated for the disease control efficacy in the field. This study showed effective P. bamuru inhibition by selective synthetic fungicides in vitro, but field efficacy remained unclear and requires further research.

真菌病原体在澳大利亚暖季草坪草上引起球道斑块,最近在新泽西州的冷季草坪草硬羊茅(短毛羊茅)上引起严重的根腐病。症状开始时为小的棕褐色斑块,但可发展为不规则的环状坏死草皮,直径可达3米以上。对这种疾病的控制知之甚少,在该领域有一些关于单一和多种活性成分产品无效的报道。为了更好地鉴定出有效的杀真菌剂,对39种杀真菌剂的体外抑菌效果进行了评价。在10%马铃薯葡萄糖琼脂培养基中加入杀菌剂,在每个平板中央放置一个bamuru菌丝塞,测定菌丝径向生长情况。观察到不同品种的生长情况,表明不同品种对杀菌剂的敏感性不同。隶属于5个化学科的12种杀菌剂完全抑制了bamuru的生长。然后对其中10种杀菌剂进行了田间防病效果评价。本研究表明,选择性合成杀菌剂在体外可有效抑制bamuru,但田间效果尚不清楚,需要进一步研究。
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引用次数: 0
Salinity affects root growth of container grown saltgrass 盐度对容器盐草根系生长有影响
Pub Date : 2025-05-05 DOI: 10.1002/its2.70055
Jason S. Young, Louise H. Comas, Yaling Qian

The need for salinity tolerant turfgrasses is increasing because of the growing use of effluent or other low-quality waters for turfgrass irrigation. Salinity can affect soils and plants in all climates but is particularly common in arid environments and has numerous causes. Salinity impacts the growth of most plants, albeit with variations among species. The objective of this experiment was to quantify the effects of salinity on root growth of an experimental line of saltgrass (Distichlis spicata L. Greene) (SG) and NuGlade Kentucky bluegrass (Poa pratensis L.) (KBG), two grasses with contrasting salinity tolerance. In this experiment, SG and KBG were grown in containers within separate growth chambers, each maintained at their optimal growing temperatures. Salinity was imposed on the plants by irrigating with saline waters of ∼0, 8, and 16 dS/m for SG and ∼0, 4, and 8 dS/m for KBG. Root growth was monitored using minirhizotron and in-growth root cores. The data from in-growth root cores revealed contrasting effects of salinity on root and rhizome growth trends between SG and KBG. Root growth of SG increased by 22% under 8 and 16 dS/m salinity treatments. Conversely, salinity treatment of 8 dS/m decreased KBG root growth by 33% compared to the control. Saltgrass rhizome growth increased by 130% with the rise in salinity from the control to 16 dS/m treatment, whereas KBG rhizome growth decreased by 57% as salinity increased from the control to 8 dS/m. Minirhizotron observation indicated that SG showed increased flushes of fine roots in the moderate levels of salinity (8 dS/m) compared to the control about 3 weeks after salt treatments began. Increased fine root production greatly increased the root surface area for absorption, an adaptive response of SG to salinity. Turf quality did not deteriorate by even the highest level of salinity tested in saltgrass, suggesting it is a good candidate for turf growing under saline conditions.

由于越来越多地使用污水或其他低质量的水灌溉草坪草,对耐盐草坪草的需求正在增加。盐碱化可以影响所有气候条件下的土壤和植物,但在干旱环境中尤为常见,并有许多原因。盐度影响大多数植物的生长,尽管不同物种之间存在差异。本试验旨在量化盐度对盐草(Distichlis spicata L. Greene)和NuGlade Kentucky蓝草(Poa pratensis L.)试验品系根系生长的影响。(KBG),两种耐盐性不同的草。在本试验中,SG和KBG分别在不同的培养皿中生长,并保持各自的最佳生长温度。对SG和KBG分别用~ 0、8和16 dS/m的盐水灌溉,对植株施加盐度。采用微型植管和生长中根芯监测根系生长情况。生长中的根芯数据揭示了盐度对SG和KBG根和根茎生长趋势的影响。在8和16 dS/m的盐度处理下,SG的根系生长增加了22%。相反,与对照相比,8 dS/m的盐度处理使KBG根生长减少了33%。从对照到16 dS/m,盐草根茎的生长随盐度的增加而增加130%,而从对照到8 dS/m,盐草根茎的生长随盐度的增加而减少57%。微硝唑观察表明,盐处理开始约3周后,在中等盐度(8 dS/m)条件下,SG细根冲水量较对照有所增加。细根产量的增加大大增加了根的吸收表面积,这是SG对盐度的适应性响应。即使在盐草中测试的最高盐度水平下,草皮质量也没有恶化,这表明它是在盐水条件下生长草皮的良好候选者。
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引用次数: 0
Branding with marketing trademarks adds another layer of complexity to traditional botanical taxonomy and nomenclature 品牌与营销商标增加了另一层复杂性,传统的植物分类和命名
Pub Date : 2025-05-01 DOI: 10.1002/its2.70030
Donald S. Loch

Fundamental to writing a scientific paper is the need to define accurately the plant and other materials used in an experiment. The basics of long-standing international rules which define plant species and cultivar names (the International Code of Botanical Nomenclature and the International Code of Nomenclature for Cultivated Plants) are briefly explained. Based on the standard of nomenclature used in manuscripts submitted to recent International Turfgrass Research Conferences, these rules appear to be poorly understood and applied nowadays by many plant scientists. Additionally and over the past 25–30 years, the commercial use of branding (via trademark names) to market turfgrasses, especially warm-season varieties, has increased rapidly, but universities have been slow to pick up on this trend, research how branding operates and its effect—beneficial or otherwise—for both marketers and consumers, and add it as a curriculum topic to improve the understanding of its nomenclatural implications by scientists. The properties of trademarks and protected cultivar names are described, together with how these two very different, yet complementary, forms of intellectual property interact commercially through branding to market plant varieties. Current and future options and opportunities with branding are briefly discussed.

写一篇科学论文的基础是需要准确地定义实验中使用的植物和其他材料。简要解释了定义植物物种和栽培品种名称的长期国际规则(国际植物命名法和国际栽培植物命名法)的基础知识。根据最近提交给国际草坪草研究会议的手稿中使用的命名标准,这些规则似乎很少被许多植物科学家理解和应用。此外,在过去的25-30年里,品牌的商业用途(通过商标名称)来销售草坪草,特别是暖季品种,迅速增加,但大学在这方面进展缓慢,研究品牌是如何运作的,以及它对营销人员和消费者的影响是有益的还是有害的,并将其作为一个课程主题,以提高科学家对其命名含义的理解。介绍了商标和受保护的品种名称的属性,以及这两种截然不同但又互补的知识产权形式如何通过品牌营销植物品种而在商业上相互作用。简要讨论了当前和未来的品牌选择和机会。
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引用次数: 0
Responses of new Cynodon genotypes to prolonged and recurrent drought 新Cynodon基因型对长期和反复干旱的响应
Pub Date : 2025-05-01 DOI: 10.1002/its2.70034
Christian S. Bowman, Marta Pudzianowska, James H. Baird

Increasing droughts coupled with decreasing available water resources in the southwestern United States highlight the need for new turfgrass cultivars that require less water. A field study evaluated a set of experimental and commercial bermudagrasses (Cynodon Rich. species) for responses to prolonged and repeated drought conditions in Riverside, CA (USDA Hardiness Zone 10a). Irrigation was withheld for two successive 60-day cycles of drought, each followed by 14-day recovery periods in 2020, 2021, and 2022. Plots were evaluated weekly for turf quality and leaf firing, as well as living green coverage, normalized difference vegetation index, and dark green color index using digital imagery. Using living green coverage as a metric for drought performance, seven experimental genotypes (UCRC180012, UCRC180037, UCRC180040, UCRC180146, UCRC180217, UCRC180229, and UCRC180557) were consistently among the top 10 performers, on average retaining over 50% green coverage by the end of each dry-down cycle. In comparison, all check bermudagrass [Cynodon dactylon (L.) Pers.] (Riley's Super Sport [Celebration®]) and hybrid bermudagrass (C. dactylon × C. transvaalensis Burtt Davy) (Bandera®, Santa Ana, DT-1 [TifTuf®], Tifway II) cultivars had low to moderate responses, ranging from 36% to 0% green coverage. Riley's Super Sport and DT-1 ranked the highest among check cultivars across years and cycles. Some genotypes appeared to show stress memory, where the first drought period in a year appeared to prime them for a more successful second period each year. Results demonstrate extensive variation among bermudagrasses (Cynodon spp.) in response to drought and that evaluation over repeated drought cycles appears to be a useful selection tool for Mediterranean climates.

美国西南部日益严重的干旱加上可用水资源的减少,突出了对需要更少水的新型草坪草品种的需求。一项实地研究评估了一套实验和商业百慕大草(Cynodon Rich)。物种)对河滨长期和反复干旱条件的反应,CA (USDA耐寒区10a)。在连续两个60天的干旱周期中,灌溉被暂停,每个周期在2020年、2021年和2022年分别有14天的恢复期。利用数字图像,每周对地块进行草坪质量和叶片燃烧、活绿覆盖率、归一化植被指数和深绿色指数的评估。将绿色覆盖度作为干旱表现的指标,7个实验基因型(UCRC180012、UCRC180037、UCRC180040、UCRC180146、UCRC180217、UCRC180229和UCRC180557)一直名列前10名,在每个干旱周期结束时平均保持50%以上的绿色覆盖度。相比之下,所有的检查百慕大草[Cynodon dactylon (L.)]。珀耳斯。] (Riley's Super Sport [Celebration®])和杂交百慕大草(C. dactylon × C. transvaalensis Burtt Davy) (Bandera®,Santa Ana, DT-1 [TifTuf®],Tifway II)品种的绿色覆盖率为36%至0%,反应较低至中等。莱利的超级运动和DT-1在不同年份和周期的对照品种中排名最高。一些基因型似乎表现出压力记忆,一年中第一次干旱似乎为它们每年更成功的第二次干旱做好了准备。结果表明,百慕大草(Cynodon spp.)对干旱的反应存在广泛的差异,对重复干旱周期的评估似乎是地中海气候的有用选择工具。
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引用次数: 0
Chlorophyll fluorescence characteristics of turfgrasses grown under shade trees 遮荫下草坪草的叶绿素荧光特性
Pub Date : 2025-05-01 DOI: 10.1002/its2.70032
David Jespersen, Somerville Rowe

Shade is a widespread environmental condition that results in the decline of quality in turfgrasses. There is a great need to understand how plants physiologically respond to shaded conditions, and more specifically the ability of plants to harvest light energy in these limited light conditions. A collection of cool-season (hybrid bluegrass [Poa arachnifera × Poa pratensis] and tall fescue [Festuca arundinaceae]) and warm-season turfgrasses (bermudagrass [Cynodon dactylon × Cynodon transvalensis], seashore paspalum [Paspalum vaginatum], and Zoysia spp.) were grown under full-sun or tree shade environments. In addition to estimates of overall performance, chlorophyll fluorescence parameters were used to estimate photochemical processes associated with light harvesting. Differences in shade performance were detected along with changes to photosynthetic processes. Rankings of shade tolerance were similar to previous research, with cool-season species being relatively more tolerant, followed by zoysiagrasses, and bermudagrass being relatively shade sensitive. Based on chlorophyll fluorescence and the JIP-test, a plant's ability to capture electrons may be more critical for shade tolerance than their ability to perform intersystem electron transport. Chlorophyll fluorescence parameters are a potentially useful tool to further explore plant adaptation to shaded conditions.

遮荫是导致草坪草质量下降的普遍环境条件。我们非常需要了解植物对阴影条件的生理反应,更具体地说,是植物在这些有限的光照条件下获取光能的能力。在充分阳光或树荫的环境下,种植了冷季蓝草(杂交蓝草[Poa arachnifera × Poa pratensis]和高羊茅[Festuca arundinaceae])和暖季草坪草(bermograss [Cynodon dactylon × Cynodon transvalensis]、海滨雀稗[paspalum vaginatum]和结尾草spp.)。除了估计总体性能外,叶绿素荧光参数还用于估计与光收获相关的光化学过程。遮荫性能的差异与光合过程的变化一起被检测到。耐荫性的排名与之前的研究相似,冷季物种相对更耐荫,其次是结缕草,百慕大草相对对荫敏感。基于叶绿素荧光和jip测试,植物捕获电子的能力可能比它们进行系统间电子传递的能力更重要。叶绿素荧光参数是一个潜在的有用的工具,进一步探讨植物适应阴影条件。
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引用次数: 0
期刊
International Turfgrass Society Research Journal
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