Pub Date : 2001-10-01DOI: 10.1046/J.1443-9573.2001.00061.X
Tong Shi, Wenzhong Liu, S. Xiao, Weiwen Xu
OBJECTIVE: To investigate the prevalences of metronidazole- and clarithromycin-resistant Helicobacter pylori over the period from 1995 to 1999 in Shanghai, and the molecular mechanism of resistance to clarithromycin. METHODS: A total of 150 H. pylori strains were randomly selected from the isolates collected in 1995, 1997 and 1999, and tested for sensitivity against metronidazole and clarithromycin by using the E-test. The mechanism of resistance was studied by polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP). RESULTS: It was found that 42% (21/50), 57% (27/50) and 70% (35/50) of the tested strains were resistant to metronidazole among the isolates collected in 1995, 1997 and 1999, respectively. In 1995, there was no strain (0/50) resistant to clarithromycin, of which the prevalence rose to 2% (1/50) in 1997, and to 10% (5/50) in 1999. The prevalences of metronidazole- and clarithromycin-resistant H. pylori in 1999 were significantly higher than those in 1995 (P < 0.05). Of nine clarithromycin-resistant H. pylori strains, eight were found to have an AG mutation at position 2144 of domain V of the 23S rRNA. CONCLUSIONS: These results suggest a significant increase in the prevalences of metronidazole- and clarithromycin-resistant H. pylori in Shanghai during the 1995–1999 period. The majority (88.8%) of clarithromycin-resistant H. pylori isolates have an A2144G mutation in domain V of the 23S rRNA.
{"title":"Prevalences of metronidazole- and clarithromycin-resistant Helicobacter pylori isolates in Shanghai and molecular mechanism of resistance to clarithromycin","authors":"Tong Shi, Wenzhong Liu, S. Xiao, Weiwen Xu","doi":"10.1046/J.1443-9573.2001.00061.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00061.X","url":null,"abstract":"OBJECTIVE: To investigate the prevalences of metronidazole- and clarithromycin-resistant Helicobacter pylori over the period from 1995 to 1999 in Shanghai, and the molecular mechanism of resistance to clarithromycin. \u0000 \u0000 \u0000 \u0000METHODS: A total of 150 H. pylori strains were randomly selected from the isolates collected in 1995, 1997 and 1999, and tested for sensitivity against metronidazole and clarithromycin by using the E-test. The mechanism of resistance was studied by polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP). \u0000 \u0000 \u0000 \u0000RESULTS: It was found that 42% (21/50), 57% (27/50) and 70% (35/50) of the tested strains were resistant to metronidazole among the isolates collected in 1995, 1997 and 1999, respectively. In 1995, there was no strain (0/50) resistant to clarithromycin, of which the prevalence rose to 2% (1/50) in 1997, and to 10% (5/50) in 1999. The prevalences of metronidazole- and clarithromycin-resistant H. pylori in 1999 were significantly higher than those in 1995 (P < 0.05). Of nine clarithromycin-resistant H. pylori strains, eight were found to have an AG mutation at position 2144 of domain V of the 23S rRNA. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: These results suggest a significant increase in the prevalences of metronidazole- and clarithromycin-resistant H. pylori in Shanghai during the 1995–1999 period. The majority (88.8%) of clarithromycin-resistant H. pylori isolates have an A2144G mutation in domain V of the 23S rRNA.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73246758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-10-01DOI: 10.1046/J.1443-9573.2001.00057.X
Tian-shu Liu, Ji-yao Wang, Shiyao Chen, Fan Yue
OBJECTIVE: To study the relationship between Helicobacter pylori eradication and the pathological development of the gastric mucosa in H. pylori-related diseases. METHODS: One hundred and ninety-one H. pylori-infected patients were randomly given anti-H. pylori or non-anti-H. pylori medications. Endoscopic examination was carried out 1 year after treatment. Pathological classifications followed the Sydney System. RESULTS: Of the 191 patients, those with chronic inflammation of the gastric mucosa improved (P < 0.05), as did those with atrophy and intestinal metaplasia (P < 0.05). Helicobacter pylori was eradicated in 107 patients, but not in 84 patients. Compared with those patients in whom H. pylori was not eradicated, those with H. pylori eradicated had ameliorated chronic inflammation of the gastric mucosa (P < 0.05) and active inflammation reduced in some cases (P < 0.05). Notwithstanding a stratification of different gastric diseases and different treatments, patients with H. pylori eradicated showed a more marked improvement in mucosal chronic inflammation than did patients in whom H. pylori was not eradicated (P < 0.05). CONCLUSIONS: These results suggest that H. pylori infection is closely related to active inflammation of the gastric mucosa. Helicobacter pylori eradication is beneficial in improving chronic inflammation of the gastric mucosa.
{"title":"Pathological development of the gastric mucosa in Helicobacter pylori-related diseases","authors":"Tian-shu Liu, Ji-yao Wang, Shiyao Chen, Fan Yue","doi":"10.1046/J.1443-9573.2001.00057.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00057.X","url":null,"abstract":"OBJECTIVE: To study the relationship between Helicobacter pylori eradication and the pathological development of the gastric mucosa in H. pylori-related diseases. \u0000 \u0000 \u0000 \u0000METHODS: One hundred and ninety-one H. pylori-infected patients were randomly given anti-H. pylori or non-anti-H. pylori medications. Endoscopic examination was carried out 1 year after treatment. Pathological classifications followed the Sydney System. \u0000 \u0000 \u0000 \u0000RESULTS: Of the 191 patients, those with chronic inflammation of the gastric mucosa improved (P < 0.05), as did those with atrophy and intestinal metaplasia (P < 0.05). Helicobacter pylori was eradicated in 107 patients, but not in 84 patients. Compared with those patients in whom H. pylori was not eradicated, those with H. pylori eradicated had ameliorated chronic inflammation of the gastric mucosa (P < 0.05) and active inflammation reduced in some cases (P < 0.05). Notwithstanding a stratification of different gastric diseases and different treatments, patients with H. pylori eradicated showed a more marked improvement in mucosal chronic inflammation than did patients in whom H. pylori was not eradicated (P < 0.05). \u0000 \u0000 \u0000 \u0000CONCLUSIONS: These results suggest that H. pylori infection is closely related to active inflammation of the gastric mucosa. Helicobacter pylori eradication is beneficial in improving chronic inflammation of the gastric mucosa.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78384850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-10-01DOI: 10.1046/J.1443-9573.2001.00059.X
Jian-hang Leng, Lihuang Zhang, H. Yao, Xuetao Cao
OBJECTIVE: Cancer immunotherapy and gene therapy are attractive approaches that have been widely investigated in different types of cancer. The present study examined the therapeutic effects of intrasplenically transplanted γ-interferon (IFN-γ) gene-modified hepatocytes on murine implanted liver carcinoma. METHODS: Embryonic murine hepatocytes (BNL.CL2) were transfected with a recombinant adenovirus encoding IFN-γ; two cell lines, BNL.LacZ and BNL.CL2, were used as controls. One week after intrasplenic C26 (colon carcinoma) cells were injected, 60 tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IFN-γ gene-modified hepatocytes and were divided into treatment (BNL.IFN-γ) and control groups (BNL.LacZ and BNL.CL2). Two weeks later, the serum levels of IFN-γ, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of splenic cytotoxic T lymphocytes (CTL) were measured, and the mor-phology of the hepatic tumors was studied to evaluate the therapeutic effects of the treatment. RESULTS: In the treatment group, the serum levels of IFN-γ, TNF-α and NO increased significantly (P < 0.01), and the splenic CTL activity also increased markedly (P < 0.01), accompanied by a substantial decrease in tumor volume and an increase in survival. CONCLUSIONS: These data indicate that the IFN-γ recombinant adenovirus was able to exert potent therapeutic effects and that the intrasplenic transplantation of adenovirus-mediated IFN-γ gene- modified hepatocytes could be used as a treatment for implanted liver carcinoma.
{"title":"Effects of γ‐interferon gene‐modified hepatocytes on murine implanted liver carcinoma","authors":"Jian-hang Leng, Lihuang Zhang, H. Yao, Xuetao Cao","doi":"10.1046/J.1443-9573.2001.00059.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00059.X","url":null,"abstract":"OBJECTIVE: Cancer immunotherapy and gene therapy are attractive approaches that have been widely investigated in different types of cancer. The present study examined the therapeutic effects of intrasplenically transplanted γ-interferon (IFN-γ) gene-modified hepatocytes on murine implanted liver carcinoma. \u0000 \u0000 \u0000 \u0000METHODS: Embryonic murine hepatocytes (BNL.CL2) were transfected with a recombinant adenovirus encoding IFN-γ; two cell lines, BNL.LacZ and BNL.CL2, were used as controls. One week after intrasplenic C26 (colon carcinoma) cells were injected, 60 tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IFN-γ gene-modified hepatocytes and were divided into treatment (BNL.IFN-γ) and control groups (BNL.LacZ and BNL.CL2). Two weeks later, the serum levels of IFN-γ, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of splenic cytotoxic T lymphocytes (CTL) were measured, and the mor-phology of the hepatic tumors was studied to evaluate the therapeutic effects of the treatment. \u0000 \u0000 \u0000 \u0000RESULTS: In the treatment group, the serum levels of IFN-γ, TNF-α and NO increased significantly (P < 0.01), and the splenic CTL activity also increased markedly (P < 0.01), accompanied by a substantial decrease in tumor volume and an increase in survival. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: These data indicate that the IFN-γ recombinant adenovirus was able to exert potent therapeutic effects and that the intrasplenic transplantation of adenovirus-mediated IFN-γ gene- modified hepatocytes could be used as a treatment for implanted liver carcinoma.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91152473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00045.X
Xiao-Bo Li, Wenzhong Liu, Weiwen Xu, Yao Shi, S. Xiao
OBJECTIVE: The cag pathogenicity island (cag PAI) has been reported to be the major factor controlling the virulence of Helicobacter pylori and has been associated with clinical outcome after H. pylori infection. The role of cag PAI genes in the pathogenicity of H. pylori infection remains to be established. The aim of the present study was to investigate: (i) the prevalence of cagA, cagE and cagT, the representative genes of cag PAI status, in H. pylori strains isolated from patients with various gastroduodenal diseases in the Shanghai region; and (ii) the relationship between the presence of these genes and different gastroduodenal diseases. METHODS: Ninety-nine H. pylori strains were isolated from patients with chronic superficial gastritis (17), chronic atrophic gastritis (21), gastric ulcers (19), duodenal ulcers (23) and gastric cancer (19). The prevalence of the cagA, cagE, and cagT genes was studied in these strains by using the polymerase chain reaction (PCR) method. RESULTS: The total prevalences of cagA, cagE and cagT genes were 84.8, 99 and 84.8%, respectively, in the 99 strains of H. pylori tested. There was no significant difference in the prevalence of cagA, cagE or cagT in H. pylori isolated from different gastroduodenal diseases (all P > 0.05). Of the 98 cagE-positive isolates, 14 (14.3%) were cagA negative. Only one of 99 isolates was negative for both cagE and cagA. Of the 84 cagT-positive strains, two were cagA negative. CONCLUSIONS: Most of the H. pylori isolates in the Shanghai region may have intact cag PAI. It was found that cagE, but not cagA, could be used as a marker for the presence of cag PAI. There is no correlation between the integrity of cag PAI and the clinical outcome after infection with H. pylori.
{"title":"Clinical implications and prevalence of cagA, cagE and cagT genes in the pathogenicity island of Helicobacter pylori strains isolated from Shanghai patients","authors":"Xiao-Bo Li, Wenzhong Liu, Weiwen Xu, Yao Shi, S. Xiao","doi":"10.1046/J.1443-9573.2001.00045.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00045.X","url":null,"abstract":"OBJECTIVE: The cag pathogenicity island (cag PAI) has been reported to be the major factor controlling the virulence of Helicobacter pylori and has been associated with clinical outcome after H. pylori infection. The role of cag PAI genes in the pathogenicity of H. pylori infection remains to be established. The aim of the present study was to investigate: (i) the prevalence of cagA, cagE and cagT, the representative genes of cag PAI status, in H. pylori strains isolated from patients with various gastroduodenal diseases in the Shanghai region; and (ii) the relationship between the presence of these genes and different gastroduodenal diseases. \u0000 \u0000 \u0000 \u0000METHODS: Ninety-nine H. pylori strains were isolated from patients with chronic superficial gastritis (17), chronic atrophic gastritis (21), gastric ulcers (19), duodenal ulcers (23) and gastric cancer (19). The prevalence of the cagA, cagE, and cagT genes was studied in these strains by using the polymerase chain reaction (PCR) method. \u0000 \u0000 \u0000 \u0000RESULTS: The total prevalences of cagA, cagE and cagT genes were 84.8, 99 and 84.8%, respectively, in the 99 strains of H. pylori tested. There was no significant difference in the prevalence of cagA, cagE or cagT in H. pylori isolated from different gastroduodenal diseases (all P > 0.05). Of the 98 cagE-positive isolates, 14 (14.3%) were cagA negative. Only one of 99 isolates was negative for both cagE and cagA. Of the 84 cagT-positive strains, two were cagA negative. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Most of the H. pylori isolates in the Shanghai region may have intact cag PAI. It was found that cagE, but not cagA, could be used as a marker for the presence of cag PAI. There is no correlation between the integrity of cag PAI and the clinical outcome after infection with H. pylori.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84597601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00051.X
Yu-qin Luo, Kaichun Wu, Anhua Sun, B. Pan, Xue-yong Zhang, D. Fan
OBJECTIVE: To investigate the significance of the expression of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in superficial gastritis, gastric mucosal dysplasia and gastric carcinoma, and to study the relationship between COX-2, iNOS, gastric carcinoma and Helicobacter pylori infection. METHODS: Polyclonal antibodies to COX-1, COX-2 and iNOS were used detect their expression and the status of H. pylori infection in 92 specimens of paraffin-embedded gastric tissue. Of the 92 patients, 33 had superficial gastritis, 30 had gastric mucosal dysplasia and 29 had gastric cancer. Helicobacter pylori was detected by toluidine blue staining. RESULTS: Expression of COX-2 and iNOS in gastric cancer (65.5%, 62.1%) was significantly higher than that in gastritis (18.2%, 18.2%; P < 0.01). Expression of COX-2 and iNOS in gastritis with H. pylori infection was higher than that in gastric mucosal dysplasia with H. pylori infection. The expression of COX-2 and iNOS occurred concomitantly in gastritis, dysplasia and gastric cancer. CONCLUSION: Inflammation and H. pylori infection may be able to stimulate the expression of COX-2 and iNOS, which might be involved in gastric carcinogenesis.
{"title":"Expression of COX‐1, COX‐2 and inducible nitric oxide synthase in superficial gastritis, mucosal dysplasia and gastric carcinoma","authors":"Yu-qin Luo, Kaichun Wu, Anhua Sun, B. Pan, Xue-yong Zhang, D. Fan","doi":"10.1046/J.1443-9573.2001.00051.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00051.X","url":null,"abstract":"OBJECTIVE: To investigate the significance of the expression of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in superficial gastritis, gastric mucosal dysplasia and gastric carcinoma, and to study the relationship between COX-2, iNOS, gastric carcinoma and Helicobacter pylori infection. \u0000 \u0000 \u0000 \u0000METHODS: Polyclonal antibodies to COX-1, COX-2 and iNOS were used detect their expression and the status of H. pylori infection in 92 specimens of paraffin-embedded gastric tissue. Of the 92 patients, 33 had superficial gastritis, 30 had gastric mucosal dysplasia and 29 had gastric cancer. Helicobacter pylori was detected by toluidine blue staining. \u0000 \u0000 \u0000 \u0000RESULTS: Expression of COX-2 and iNOS in gastric cancer (65.5%, 62.1%) was significantly higher than that in gastritis (18.2%, 18.2%; P < 0.01). Expression of COX-2 and iNOS in gastritis with H. pylori infection was higher than that in gastric mucosal dysplasia with H. pylori infection. The expression of COX-2 and iNOS occurred concomitantly in gastritis, dysplasia and gastric cancer. \u0000 \u0000 \u0000 \u0000CONCLUSION: Inflammation and H. pylori infection may be able to stimulate the expression of COX-2 and iNOS, which might be involved in gastric carcinogenesis.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91290089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00044.X
Xuan Li, Yuqin Wang, Wu-feng Cheng, Dan Wang, Feng Li
OBJECTIVE: To investigate the effects of vitamin E and different doses of selenium on the expression of the tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA in the hepatic stellate cells (HSC) of CCl4-induced hepato-fibrotic rats. The mechanism of these therapeutic actions is investigated at a molecular level. METHODS: Hepatic fibroses were induced by intraperitoneal injection of 40% CCl4 in olive oil and treated by dietary supplementation with vitamin E and different doses of selenium. Liver tissue sections were stained with routine hematoxylin and eosin staining and Masson trichrome staining for collagen. With β-actin as an internal control, the reverse transcriptase–polymerase chain reaction (RT-PCR) method was applied to quantify the change of TIMP-1 mRNA in HSC. RESULTS: The expression level of TIMP-1 mRNA in HSC was significantly downregulated and collagenous fiber proliferation in the liver was also significantly reduced in the groups of rats treated with vitamin E (250 mg/kg) and low dosages of selenium (0.2 mg/kg). However, the expression of TIMP-1 mRNA was upregulated, but not significantly, in the group treated with high dosages of selenium (1.0 mg/kg). CONCLUSIONS: The expression level of TIMP-1 mRNA in HSC was significantly downregulated and collagenous fiber proliferation in the liver was significantly reduced in the groups of rats treated with vitamin E and appropriate dosages of selenium. This did not occur in groups with high dosages of selenium.
{"title":"Effect of vitamin E and selenium on the tissue inhibitor of metalloproteinase‐1 mRNA expression in hepatic stellate cells","authors":"Xuan Li, Yuqin Wang, Wu-feng Cheng, Dan Wang, Feng Li","doi":"10.1046/J.1443-9573.2001.00044.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00044.X","url":null,"abstract":"OBJECTIVE: To investigate the effects of vitamin E and different doses of selenium on the expression of the tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA in the hepatic stellate cells (HSC) of CCl4-induced hepato-fibrotic rats. The mechanism of these therapeutic actions is investigated at a molecular level. \u0000 \u0000METHODS: Hepatic fibroses were induced by intraperitoneal injection of 40% CCl4 in olive oil and treated by dietary supplementation with vitamin E and different doses of selenium. Liver tissue sections were stained with routine hematoxylin and eosin staining and Masson trichrome staining for collagen. With β-actin as an internal control, the reverse transcriptase–polymerase chain reaction (RT-PCR) method was applied to quantify the change of TIMP-1 mRNA in HSC. \u0000 \u0000RESULTS: The expression level of TIMP-1 mRNA in HSC was significantly downregulated and collagenous fiber proliferation in the liver was also significantly reduced in the groups of rats treated with vitamin E (250 mg/kg) and low dosages of selenium (0.2 mg/kg). However, the expression of TIMP-1 mRNA was upregulated, but not significantly, in the group treated with high dosages of selenium (1.0 mg/kg). \u0000 \u0000CONCLUSIONS: The expression level of TIMP-1 mRNA in HSC was significantly downregulated and collagenous fiber proliferation in the liver was significantly reduced in the groups of rats treated with vitamin E and appropriate dosages of selenium. This did not occur in groups with high dosages of selenium.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88704365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
OBJECTIVE: To: (i) assess the clinical value of electrogastrography (EGG) and the gastric emptying test; and (ii) investigate the relationship between gastric myoelectrical activity and gastric emptying (GE). METHODS: One hundred and forty patients with functional dyspepsia (FD), 30 patients with non-insulin-dependent diabetes mellitus (NIDDM) and 20 healthy volunteers were studied. Gastric myoelectrical activity was recorded by using cutaneous EGG. The gastric emptying time was measured by using isotopic, radiopaque marker (Rom’s) and ultrasound methods. RESULTS: The dysrhythmia rates in patients with NIDDM were 70.0% (21/30) before meals and 66.7% (20/30) after meals, and the tachygastria rates of these patients were 36.7% before meals and 33.3% after meals. In NIDDM patients, the dominant frequency (DF) after meals (2.60 ± 0.30 cycles per minute; c.p.m.), the fed DF/fasting DF ratio (1.01 ± 0.11), the dominant power (DP) after meals (121.45 ± 67.00 V2 c.p.m.) and the fed DP and fasting DP ratios (0.81 ± 0.07) were significantly lower than those in normal controls (P < 0.05 or P < 0.01). The DP after meals in patients with FD (210.60 ± 68.40 V2 c.p.m.) was significantly lower than that in normal controls (P < 0.01). Delayed gastric emptying was more common in patients with FD and NIDDM. The rate of delayed gastric emptying in 121 cases with normal myoelectrical rhythm was 39.7% (48/121). In 69 cases with dysrhythmia, 45.9% (17/37) with bradygastria and 78.1% (25/32) with tachygastria had delayed gastric emptying. CONCLUSIONS: Electrogastrography and the gastric emptying test are feasible methods for evaluating gastric myoelectrical activity and gastric motility. The precise relationship between gastric myoelectrical activity and gastric motility has not been proven, but there is a close relationship between tachygastria and delayed gastric emptying.
{"title":"Clinical value of electrogastrography and the gastric emptying test","authors":"Jin-yan Luo, You-ling Zhu, Xue-qin Wang, Hui-Bin Qi","doi":"10.1046/J.1443-9573.2001.00047.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00047.X","url":null,"abstract":"OBJECTIVE: To: (i) assess the clinical value of electrogastrography (EGG) and the gastric emptying test; and (ii) investigate the relationship between gastric myoelectrical activity and gastric emptying (GE). \u0000 \u0000 \u0000 \u0000METHODS: One hundred and forty patients with functional dyspepsia (FD), 30 patients with non-insulin-dependent diabetes mellitus (NIDDM) and 20 healthy volunteers were studied. Gastric myoelectrical activity was recorded by using cutaneous EGG. The gastric emptying time was measured by using isotopic, radiopaque marker (Rom’s) and ultrasound methods. \u0000 \u0000 \u0000 \u0000RESULTS: The dysrhythmia rates in patients with NIDDM were 70.0% (21/30) before meals and 66.7% (20/30) after meals, and the tachygastria rates of these patients were 36.7% before meals and 33.3% after meals. In NIDDM patients, the dominant frequency (DF) after meals (2.60 ± 0.30 cycles per minute; c.p.m.), the fed DF/fasting DF ratio (1.01 ± 0.11), the dominant power (DP) after meals (121.45 ± 67.00 V2 c.p.m.) and the fed DP and fasting DP ratios (0.81 ± 0.07) were significantly lower than those in normal controls (P < 0.05 or P < 0.01). The DP after meals in patients with FD (210.60 ± 68.40 V2 c.p.m.) was significantly lower than that in normal controls (P < 0.01). Delayed gastric emptying was more common in patients with FD and NIDDM. The rate of delayed gastric emptying in 121 cases with normal myoelectrical rhythm was 39.7% (48/121). In 69 cases with dysrhythmia, 45.9% (17/37) with bradygastria and 78.1% (25/32) with tachygastria had delayed gastric emptying. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Electrogastrography and the gastric emptying test are feasible methods for evaluating gastric myoelectrical activity and gastric motility. The precise relationship between gastric myoelectrical activity and gastric motility has not been proven, but there is a close relationship between tachygastria and delayed gastric emptying.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78508061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00041.X
Xiang-Rui Chen, W. Ren, Jufang Dong, S. Xiao, Bonnie F. Sloane
OBJECTIVE: To screen subpopulations of gastric cancer cell lines with different malignant phenotypes. METHODS: Two subpopulations from the human gastric cancer cell line MKN-45 were separated by using the laminin adhesion method. One subpopulation was less invasive and non-metastatic, whereas the other was more invasive and metastatic. The relative invasiveness and migratory capacities of the two subgroups were observed by using the Boyden chamber and by inoculating the cells into nude mice. RESULTS: The two subgroups, the laminin-adherent cells (Lm+) and the laminin non-adherent cells (Lm–), were separated. During in vitro experiments, the Lm+ cells were more invasive and their migratory ability was greater relative to the Lm– cells. The rates of tumor formation after subcutaneous inoculation in nude mice and of lung tumor foci formation after tail vein inoculation were higher in Lm+ cells than those in Lm– cells. In vivo, Lm+ cells were found to have higher metastatic potential and to be more invasive. CONCLUSIONS: In vitro, the adhesion method is a simple and time-saving way to screen a particular phenotypic cell subpopulation with a high success rate. There are discrepancies in invasiveness and migratory ability between in vitro Lm+ and Lm– cells, which suggests that these properties of gastric cancer cells are closely related to their adhesiveness to the basement membrane and extracellular matrix.
{"title":"Screening of gastric cancer cell sublines using the adhesion method","authors":"Xiang-Rui Chen, W. Ren, Jufang Dong, S. Xiao, Bonnie F. Sloane","doi":"10.1046/J.1443-9573.2001.00041.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00041.X","url":null,"abstract":"OBJECTIVE: To screen subpopulations of gastric cancer cell lines with different malignant phenotypes. \u0000 \u0000 \u0000 \u0000METHODS: Two subpopulations from the human gastric cancer cell line MKN-45 were separated by using the laminin adhesion method. One subpopulation was less invasive and non-metastatic, whereas the other was more invasive and metastatic. The relative invasiveness and migratory capacities of the two subgroups were observed by using the Boyden chamber and by inoculating the cells into nude mice. \u0000 \u0000 \u0000 \u0000RESULTS: The two subgroups, the laminin-adherent cells (Lm+) and the laminin non-adherent cells (Lm–), were separated. During in vitro experiments, the Lm+ cells were more invasive and their migratory ability was greater relative to the Lm– cells. The rates of tumor formation after subcutaneous inoculation in nude mice and of lung tumor foci formation after tail vein inoculation were higher in Lm+ cells than those in Lm– cells. In vivo, Lm+ cells were found to have higher metastatic potential and to be more invasive. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: In vitro, the adhesion method is a simple and time-saving way to screen a particular phenotypic cell subpopulation with a high success rate. There are discrepancies in invasiveness and migratory ability between in vitro Lm+ and Lm– cells, which suggests that these properties of gastric cancer cells are closely related to their adhesiveness to the basement membrane and extracellular matrix.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86060147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00048.X
J. Mo, Chengdang Wang
OBJECTIVE: To explore the effects of several oral medications on the results of the 14C-urea breath test (UBT). METHODS: One hundred and twelve patients with chronic gastritis that had tested positive in a rapid urease test were enrolled in the present study and were randomly divided into six groups: 20 patients received 20 mg famotidine b.i.d., 16 patients received 30 mg lansoprazole q.d., 18 patients received 1000 mg sucralfate t.i.d., 20 patients received 50 mg teprenone t.i.d., 20 patients received 220 mg colloidal bismuth subcitrate b.i.d., and 18 patients received four tablets t.i.d. of wei-de-an, a traditional Chinese medicine. The 14C-urea breath test was performed before treatment, 7 days after beginning drug treatment and 7 days after the cessation of drug treatment. RESULTS: All medications, especially colloidal bismuth subcitrate, were influenced by the result of the UBT. Seven days after drug treatment, the negative conversion rate of the UBT was 45.0% (9/20) in the colloidal bismuth subcitrate group, 30.0% (6/20) in the famotidine group, 31.3% (5/16) in the lansoprazole group, 16.7% (3/18) in the sucralfate group, 5.0% (1/20) in the teprenone group and 27.8% (5/18) in the wei-de-an group. However, all values returned to positive again 1 week after withdrawal. The influence of famotidine on UBT was similar to that of lansoprazole (P > 0.05). There was no significant difference between the results of the first and the third UBT. CONCLUSIONS: The UBT is a reproducible, non-invasive method for identifying Helicobacter pylori, but many drugs can influence the result of the UBT, so it is necessary to suspend treatment with these drugs before carrying out the testing procedure.
{"title":"Effects of several oral medications on the results of the 14C‐urea breath test","authors":"J. Mo, Chengdang Wang","doi":"10.1046/J.1443-9573.2001.00048.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00048.X","url":null,"abstract":"OBJECTIVE: To explore the effects of several oral medications on the results of the 14C-urea breath test (UBT). \u0000 \u0000 \u0000 \u0000METHODS: One hundred and twelve patients with chronic gastritis that had tested positive in a rapid urease test were enrolled in the present study and were randomly divided into six groups: 20 patients received 20 mg famotidine b.i.d., 16 patients received 30 mg lansoprazole q.d., 18 patients received 1000 mg sucralfate t.i.d., 20 patients received 50 mg teprenone t.i.d., 20 patients received 220 mg colloidal bismuth subcitrate b.i.d., and 18 patients received four tablets t.i.d. of wei-de-an, a traditional Chinese medicine. The 14C-urea breath test was performed before treatment, 7 days after beginning drug treatment and 7 days after the cessation of drug treatment. \u0000 \u0000 \u0000 \u0000RESULTS: All medications, especially colloidal bismuth subcitrate, were influenced by the result of the UBT. Seven days after drug treatment, the negative conversion rate of the UBT was 45.0% (9/20) in the colloidal bismuth subcitrate group, 30.0% (6/20) in the famotidine group, 31.3% (5/16) in the lansoprazole group, 16.7% (3/18) in the sucralfate group, 5.0% (1/20) in the teprenone group and 27.8% (5/18) in the wei-de-an group. However, all values returned to positive again 1 week after withdrawal. The influence of famotidine on UBT was similar to that of lansoprazole (P > 0.05). There was no significant difference between the results of the first and the third UBT. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: The UBT is a reproducible, non-invasive method for identifying Helicobacter pylori, but many drugs can influence the result of the UBT, so it is necessary to suspend treatment with these drugs before carrying out the testing procedure.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86730358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00043.X
K. Goh, P. Cheah, Ym Tan, M. Rosmawati, K. Ong, Lo Yl, S. Chin
OBJECTIVE: To determine and compare the efficacy of 5-day t.d.s and 7-day b.d. treatment regimens comprising lansoprazole, clarithromycin and amoxicillin in the eradication of Helicobacter pylori. METHODS: Patients with unequivocal evidence of H. pylori infection based on histology and rapid urease tests of both antrum and corpus biopsies were recruited for the study. The study was a randomized, investigator-blind, comparative study. Patients received either 500 mg clarithromycin t.d.s. and 500 mg amoxicillin t.d.s. for 5 days (LAC5) or 500 mg clarithromycin b.d. and 500 mg amoxicillin b.d. for 7 days (LAC7) together with 30 mg lansoprazole (both groups) daily for either 5 or 7 days, depending on the treatment group. Patients were assessed for the successful eradication of H. pylori, defined as the absence of bacteria based on histology and urease tests on both antral and corporeal biopsies, carried out at least 4 weeks after completion of the therapy. RESULTS: One hundred and eight patients were recruited for the study. In the LAC5 treatment group, four patients failed to return for follow up and in the LAC7 group, two failed to return for follow up and two were not compliant with medications. Eradication rates based on an intention-to-treat analysis were: 46/54 for LAC5 (85.2%; 95% CI = 72.9–93.4) and 47/54 for LAC7 (87.0%; 95% CI = 75.1–94.6). Based on a per protocol analysis, the rates were: 46/50 for LAC5 (92.0%; 95% CI = 80.8–97.8) and 47/50 for LAC7 (94.0%; 95% CI = 83.5–98.7). Both treatment regimens were convenient for patients and except for two patients in the LAC7 group, all patients reported taking 100% of all prescribed medications. The side-effects encountered were uniformly mild and no patient discontinued treatment because of intolerance to medications. The most common side-effects were altered taste (LAC5 64.7%; LAC7 78.8%). Diarrhea, nausea and anorexia were reported in a minority of patients. CONCLUSIONS: Both the LAC5 t.d.s. and the LAC7 b.d. treatment regimens were well tolerated by patients and were highly effective in the eradication of H. pylori.
目的:确定并比较兰索拉唑、克拉霉素和阿莫西林组成的5天t / d和7天b / d治疗方案根除幽门螺杆菌的疗效。方法:根据胃窦和体活检的组织学和快速脲酶试验，招募有明确证据的幽门螺杆菌感染的患者进行研究。该研究是一项随机、研究者盲、比较研究。患者每日接受500 mg克拉霉素和500 mg阿莫西林联合治疗5天(LAC5)或500 mg克拉霉素和500 mg阿莫西林联合治疗7天(LAC7)以及30 mg兰索拉唑(两组)治疗5天或7天，具体取决于治疗组。评估患者是否成功根除幽门螺杆菌，定义为在治疗完成后至少4周进行的胃窦和身体活检的组织学和脲酶测试中没有细菌。结果:108名患者被纳入研究。在LAC5治疗组中，4例患者未能返回随访，在LAC7治疗组中，2例患者未能返回随访，2例患者不遵医嘱。基于意向治疗分析的根除率为:LAC5为46/54 (85.2%;95% CI = 72.9-93.4)和47/54 (87.0%;95% ci = 75.1-94.6)。根据每个协议分析，LAC5的发生率为46/50 (92.0%;95% CI = 80.8-97.8)和47/50 (94.0%;95% ci = 83.5-98.7)。两种治疗方案对患者都很方便，除了LAC7组的两名患者外，所有患者都报告100%服用了所有处方药物。所遇到的副作用都很轻微，没有患者因为对药物不耐受而停止治疗。最常见的副作用是味觉改变(LAC5 64.7%;LAC7 78.8%)。少数患者出现腹泻、恶心和厌食。结论:LAC5 t.d.s.和LAC7 b.d.s.治疗方案均具有良好的耐受性，且在根除幽门螺杆菌方面非常有效。
{"title":"Efficacy of short‐course lansoprazole with clarithromycin and amoxicillin in the eradication of Helicobacter pylori in South‐East Asian patients: 5‐day t.d.s. versus 7‐day b.d. treatment regimens","authors":"K. Goh, P. Cheah, Ym Tan, M. Rosmawati, K. Ong, Lo Yl, S. Chin","doi":"10.1046/J.1443-9573.2001.00043.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00043.X","url":null,"abstract":"OBJECTIVE: To determine and compare the efficacy of 5-day t.d.s and 7-day b.d. treatment regimens comprising lansoprazole, clarithromycin and amoxicillin in the eradication of Helicobacter pylori. \u0000 \u0000 \u0000 \u0000METHODS: Patients with unequivocal evidence of H. pylori infection based on histology and rapid urease tests of both antrum and corpus biopsies were recruited for the study. The study was a randomized, investigator-blind, comparative study. Patients received either 500 mg clarithromycin t.d.s. and 500 mg amoxicillin t.d.s. for 5 days (LAC5) or 500 mg clarithromycin b.d. and 500 mg amoxicillin b.d. for 7 days (LAC7) together with 30 mg lansoprazole (both groups) daily for either 5 or 7 days, depending on the treatment group. Patients were assessed for the successful eradication of H. pylori, defined as the absence of bacteria based on histology and urease tests on both antral and corporeal biopsies, carried out at least 4 weeks after completion of the therapy. \u0000 \u0000 \u0000 \u0000RESULTS: One hundred and eight patients were recruited for the study. In the LAC5 treatment group, four patients failed to return for follow up and in the LAC7 group, two failed to return for follow up and two were not compliant with medications. Eradication rates based on an intention-to-treat analysis were: 46/54 for LAC5 (85.2%; 95% CI = 72.9–93.4) and 47/54 for LAC7 (87.0%; 95% CI = 75.1–94.6). Based on a per protocol analysis, the rates were: 46/50 for LAC5 (92.0%; 95% CI = 80.8–97.8) and 47/50 for LAC7 (94.0%; 95% CI = 83.5–98.7). Both treatment regimens were convenient for patients and except for two patients in the LAC7 group, all patients reported taking 100% of all prescribed medications. The side-effects encountered were uniformly mild and no patient discontinued treatment because of intolerance to medications. The most common side-effects were altered taste (LAC5 64.7%; LAC7 78.8%). Diarrhea, nausea and anorexia were reported in a minority of patients. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Both the LAC5 t.d.s. and the LAC7 b.d. treatment regimens were well tolerated by patients and were highly effective in the eradication of H. pylori.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82815142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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