Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00052.X
Hengjun Gao, Hongyin Zhu, Weiqi Gu, Y. Lou, W. Ren, SH Xiao
OBJECTIVE: Cancer gene therapy using interleukin-2 (IL-2) has generated much interest because of the potent antitumor effect of this cytokine. There is ongoing research into one promising new gene therapy approach for cancer using the vaccinia virus vector. The purpose of this study was to construct a vaccinia eukaryotic expression vector, pMJ601, which contains human IL-2 (hIL-2; pMJ601hIL-2) and can be used for the treatment of gastric carcinomas. METHODS: Genetic engineering techniques such as plasmid extraction, agarose gel electrophoresis, restriction analysis, ligation, preparation of competent cells, transformation and DNA sequence analysis were used to clone the hIL-2 gene into pBluescript II SK+/– and pMJ601 and identify these vectors. RESULTS: Fragments of hIL-2 DNA from pLXSN from an EcoR1–BamHI restriction enzyme digest were successfully cloned into pBluescript II SK+/–. In addition, hIL-2 DNA from pBluescript II SK+/– with a SalI-BamHI restriction enzyme digest was also successfully cloned into pMJ601. CONCLUSION: Constructing a pMJ601 vector that expresses the hIL-2 gene is an important step toward being able to treat gastric carcinoma using gene therapy.
目的:利用白细胞介素-2 (IL-2)进行肿瘤基因治疗已引起人们极大的兴趣，因为这种细胞因子具有强大的抗肿瘤作用。目前正在研究一种利用痘苗病毒载体的有前途的癌症基因治疗新方法。本研究的目的是构建牛痘真核表达载体pMJ601，该载体含有人IL-2 (IL-2;pMJ601hIL-2)，可用于胃癌的治疗。方法:采用质粒提取、琼脂糖凝胶电泳、酶切分析、连接、制备感态细胞、转化、DNA序列分析等基因工程技术，将hIL-2基因克隆到pBluescript II SK+/ -和pMJ601载体中，并进行鉴定。结果:从EcoR1-BamHI酶切酶中提取的pLXSN hIL-2 DNA片段成功克隆到pBluescript II SK+/ -中。此外，pBluescript II SK+/ -的hIL-2 DNA也通过salii - bamhi酶切成功克隆到pMJ601中。结论:构建表达hIL-2基因的pMJ601载体是实现胃癌基因治疗的重要一步。
{"title":"Construction and identification of the eukaryotic expression vector of vaccinia virus expressing human interleukin-2","authors":"Hengjun Gao, Hongyin Zhu, Weiqi Gu, Y. Lou, W. Ren, SH Xiao","doi":"10.1046/J.1443-9573.2001.00052.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00052.X","url":null,"abstract":"OBJECTIVE: Cancer gene therapy using interleukin-2 (IL-2) has generated much interest because of the potent antitumor effect of this cytokine. There is ongoing research into one promising new gene therapy approach for cancer using the vaccinia virus vector. The purpose of this study was to construct a vaccinia eukaryotic expression vector, pMJ601, which contains human IL-2 (hIL-2; pMJ601hIL-2) and can be used for the treatment of gastric carcinomas. \u0000 \u0000 \u0000 \u0000METHODS: Genetic engineering techniques such as plasmid extraction, agarose gel electrophoresis, restriction analysis, ligation, preparation of competent cells, transformation and DNA sequence analysis were used to clone the hIL-2 gene into pBluescript II SK+/– and pMJ601 and identify these vectors. \u0000 \u0000 \u0000 \u0000RESULTS: Fragments of hIL-2 DNA from pLXSN from an EcoR1–BamHI restriction enzyme digest were successfully cloned into pBluescript II SK+/–. In addition, hIL-2 DNA from pBluescript II SK+/– with a SalI-BamHI restriction enzyme digest was also successfully cloned into pMJ601. \u0000 \u0000 \u0000 \u0000CONCLUSION: Constructing a pMJ601 vector that expresses the hIL-2 gene is an important step toward being able to treat gastric carcinoma using gene therapy.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81801806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00050.X
Hong Zhu
OBJECTIVE: To evaluate the nature of persistently elevated α-fetoprotein (AFP) in a family and the importance of correct recognition and diagnosis of familial AFP elevation. METHODS: In 1984 and 1997, a series of AFP radio-immunoassays from two families with persistently elevated AFP were investigated and the family pedigrees were subanalyzed. RESULTS: Of the 29 members in the two families, 15 were examined. The AFP level of 10 people was persistently elevated, two of these had been misdiagnosed with primary hepatocellular carcinoma. CONCLUSIONS: Familial elevation of AFP is benign in nature. It should be kept in mind during mass surveys of AFP.
{"title":"Clinical symptoms and pedigree analysis in two families with benign familial persistently elevated α‐fetoprotein","authors":"Hong Zhu","doi":"10.1046/J.1443-9573.2001.00050.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00050.X","url":null,"abstract":"OBJECTIVE: To evaluate the nature of persistently elevated α-fetoprotein (AFP) in a family and the importance of correct recognition and diagnosis of familial AFP elevation. \u0000 \u0000 \u0000 \u0000METHODS: In 1984 and 1997, a series of AFP radio-immunoassays from two families with persistently elevated AFP were investigated and the family pedigrees were subanalyzed. \u0000 \u0000 \u0000 \u0000RESULTS: Of the 29 members in the two families, 15 were examined. The AFP level of 10 people was persistently elevated, two of these had been misdiagnosed with primary hepatocellular carcinoma. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Familial elevation of AFP is benign in nature. It should be kept in mind during mass surveys of AFP.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88771014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00049.X
Shu Zhang, Mei-Jie Hu, Yuxin Wu, S. Jiang, Yunlin Wu, Yao-zong Yuan
OBJECTIVE: To study: (i) the induction of apoptosis in gastric cancer cells by trichosanthin; and (ii) the relationship between apoptosis and the expression of bcl-2. METHODS: During in vitro experiments, morphological studies and the terminal deoxynucleotidyl transferase-mediated dUTP–digoxigenin nick end-labeling (TUNEL) method were used to detect apoptosis in gastric adenocarcinoma cell line SGC-7901 before and after trichosanthin treatment. An immunohistochemical staining method and northern blot hybridization were used to detect the expression of the apoptosis-related gene bcl-2 before and after trichosanthin treatment. RESULTS: When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 36 h), they presented some typical apoptotic morphological changes that were observed by fluorescent staining. These morphological changes included nuclear condensation and nucleosomal fragments forming a lunate body under the nuclear membrane. When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL) for 36, 42 or 48 h, TUNEL staining revealed a significant increase in the apoptotic index (AI), from 3.78 ± 1.11%, 3.98 ± 1.12% and 3.85 ± 1.08%, to 11.30 ± 2.33%, 10.22 ± 2.00% and 11.18 ± 1.85% (P < 0.01), respectively. When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 32 h), immunohistochemical staining revealed a decreased expression of the bcl-2 protein product: the staining density decreased from ++/+++ to –/+ (P < 0.01). When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 24 h), northern blot hybridization showed a decreased expression of bcl-2 RNA: hybridization decreased from 35.19 ± 2.34 to 22.27 ± 3.90 (P < 0.01). CONCLUSIONS: Trichosanthin is able to induce apoptosis in gastric cancer. The apoptosis may be mediated by the downexpression of the apoptosis-related gene bcl-2.
{"title":"Trichosanthin‐induced apoptosis in gastric cancer is related to the downexpression of bcl‐2","authors":"Shu Zhang, Mei-Jie Hu, Yuxin Wu, S. Jiang, Yunlin Wu, Yao-zong Yuan","doi":"10.1046/J.1443-9573.2001.00049.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00049.X","url":null,"abstract":"OBJECTIVE: To study: (i) the induction of apoptosis in gastric cancer cells by trichosanthin; and (ii) the relationship between apoptosis and the expression of bcl-2. \u0000 \u0000 \u0000 \u0000METHODS: During in vitro experiments, morphological studies and the terminal deoxynucleotidyl transferase-mediated dUTP–digoxigenin nick end-labeling (TUNEL) method were used to detect apoptosis in gastric adenocarcinoma cell line SGC-7901 before and after trichosanthin treatment. An immunohistochemical staining method and northern blot hybridization were used to detect the expression of the apoptosis-related gene bcl-2 before and after trichosanthin treatment. \u0000 \u0000 \u0000 \u0000RESULTS: When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 36 h), they presented some typical apoptotic morphological changes that were observed by fluorescent staining. These morphological changes included nuclear condensation and nucleosomal fragments forming a lunate body under the nuclear membrane. When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL) for 36, 42 or 48 h, TUNEL staining revealed a significant increase in the apoptotic index (AI), from 3.78 ± 1.11%, 3.98 ± 1.12% and 3.85 ± 1.08%, to 11.30 ± 2.33%, 10.22 ± 2.00% and 11.18 ± 1.85% (P < 0.01), respectively. When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 32 h), immunohistochemical staining revealed a decreased expression of the bcl-2 protein product: the staining density decreased from ++/+++ to –/+ (P < 0.01). When SGC-7901 cells were treated with trichosanthin (0.1 μg/mL, 24 h), northern blot hybridization showed a decreased expression of bcl-2 RNA: hybridization decreased from 35.19 ± 2.34 to 22.27 ± 3.90 (P < 0.01). \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Trichosanthin is able to induce apoptosis in gastric cancer. The apoptosis may be mediated by the downexpression of the apoptosis-related gene bcl-2.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74679072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00042.X
Yan Gu, Jian-qun Xie, Zhao-han Wu, H. Zhuo
OBJECTIVE: To study the effects and the underlying mechanism of recombinant human growth hormone (rhGH) and glutamine (Gln) on the adaptation of the remnant small intestine in parenterally nourished, short bowel syndrome (SBS) rats. METHOD: Four parenteral nutrition (PN) treatment groups of SBS rats were randomly arranged in a 2 × 2 factorial design as follows: (i) STD (standard PN) group (–rhGH, –Gln); (ii) Gln group (–rhGH, +Gln); (iii) rhGH group (+rhGH, –Gln); and (iv) rhGH + Gln group (+rhGH, +Gln). Morphological changes of the intestinal mucosa were investigated and the expression of proliferating cell nuclear antigen (PCNA) and the occurrence of apoptosis were observed by immunohistochemical staining and terminal deoxynucleotidyl transfer-mediated dUTP-biotin nick end-labeling (TUNEL) methods. The level of intestinal insulin-like growth factor-1 (IGF-1) mRNA was determined by northern blotting. RESULTS: The mucosal thickness, villous height, crypt depth and villous surface area of the remnant small intestine in the rhGH + Gln group were increased significantly as compared with the other three experimental groups, and there were synergistic effects between rhGH and Gln (P < 0.01). The expression of PCNA was higher in the rhGH + Gln group than in the rhGH, Gln and STD groups (24.95 ± 3.93 vs 19.28 ± 3.25, 17.27 ± 3.38, and 8.37 ± 2.23 positive cells per crypt of Lieberkuhn, respectively; P < 0.01) but the rate of apoptosis was lower in the rhGH + Gln group than in the rhGH, Gln and STD groups (5.68 ± 2.07 vs 8.06 ± 2.33, 10.00 ± 2.24 and 22.32 ± 3.84 positive cells per 100 cells, respectively; P < 0.01). The intestinal IGF-1 mRNA was also expressed at a higher level in the rhGH + Gln group than in the rhGH, Gln and STD groups (0.73 ± 0.05 vs 0.62 ± 0.04 vs 0.51 ± 0.04 and 0.41 ± 0.22, respectively; P < 0.05). CONCLUSION: The synergistic combination of rhGH and Gln can significantly improve the adaptation of the remnant small intestine in parenterally fed SBS rats. An increase in cell proliferation and a decrease in cell apoptosis are both responsible for the intestinal adaptation. An increase in local IGF-1 plays an important role in this process.
{"title":"Recombinant human growth hormone and glutamine synergistically improve the adaptation of the remnant small intestine in rats with short bowel syndrome","authors":"Yan Gu, Jian-qun Xie, Zhao-han Wu, H. Zhuo","doi":"10.1046/J.1443-9573.2001.00042.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00042.X","url":null,"abstract":"OBJECTIVE: To study the effects and the underlying mechanism of recombinant human growth hormone (rhGH) and glutamine (Gln) on the adaptation of the remnant small intestine in parenterally nourished, short bowel syndrome (SBS) rats. \u0000 \u0000 \u0000 \u0000METHOD: Four parenteral nutrition (PN) treatment groups of SBS rats were randomly arranged in a 2 × 2 factorial design as follows: (i) STD (standard PN) group (–rhGH, –Gln); (ii) Gln group (–rhGH, +Gln); (iii) rhGH group (+rhGH, –Gln); and (iv) rhGH + Gln group (+rhGH, +Gln). Morphological changes of the intestinal mucosa were investigated and the expression of proliferating cell nuclear antigen (PCNA) and the occurrence of apoptosis were observed by immunohistochemical staining and terminal deoxynucleotidyl transfer-mediated dUTP-biotin nick end-labeling (TUNEL) methods. The level of intestinal insulin-like growth factor-1 (IGF-1) mRNA was determined by northern blotting. \u0000 \u0000 \u0000 \u0000RESULTS: The mucosal thickness, villous height, crypt depth and villous surface area of the remnant small intestine in the rhGH + Gln group were increased significantly as compared with the other three experimental groups, and there were synergistic effects between rhGH and Gln (P < 0.01). The expression of PCNA was higher in the rhGH + Gln group than in the rhGH, Gln and STD groups (24.95 ± 3.93 vs 19.28 ± 3.25, 17.27 ± 3.38, and 8.37 ± 2.23 positive cells per crypt of Lieberkuhn, respectively; P < 0.01) but the rate of apoptosis was lower in the rhGH + Gln group than in the rhGH, Gln and STD groups (5.68 ± 2.07 vs 8.06 ± 2.33, 10.00 ± 2.24 and 22.32 ± 3.84 positive cells per 100 cells, respectively; P < 0.01). The intestinal IGF-1 mRNA was also expressed at a higher level in the rhGH + Gln group than in the rhGH, Gln and STD groups (0.73 ± 0.05 vs 0.62 ± 0.04 vs 0.51 ± 0.04 and 0.41 ± 0.22, respectively; P < 0.05). \u0000 \u0000 \u0000 \u0000CONCLUSION: The synergistic combination of rhGH and Gln can significantly improve the adaptation of the remnant small intestine in parenterally fed SBS rats. An increase in cell proliferation and a decrease in cell apoptosis are both responsible for the intestinal adaptation. An increase in local IGF-1 plays an important role in this process.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74289103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-07-01DOI: 10.1046/J.1443-9573.2001.00046.X
Hong Lu, Z. Ge, Wenzhong Liu, Xiao-yu Chen, Yanshen Peng, S. Xiao
OBJECTIVE: Several epidemiological studies have indicated that the long-term administration of non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the incidence of colorectal cancer. The best known action of NSAIDs is to block cyclooxygenase, the key enzyme required for the conversion of arachidonic acid to prostaglandins. Two cyclooxygenase isoforms have been identified and these are referred to as COX-1 and COX-2. Recent studies indicate that inducible COX-2 plays an important role in gastrointestinal inflammation and carcinogenesis. The present study was undertaken to investigate the expression and clinical implications of COX-2 and COX-1 in normal and diseased colons. METHODS: COX-2 and COX-1 protein expression in specimens from normal controls and diseased colon tissues were examined semiquantitatively by using immunohistochemical methods. RESULTS: By using immunohistochemical detection methods, low COX-2 protein expression in colonic epithelial cells was observed in 20.0% (2/10) of normal controls. Eighty percent (16/20) of specimens from inflammatory bowel disease (IBD) had a high COX-2 expression, 46.40% (13/28) of adenomas and 64.3% of (9/14) well-differentiated colonic carcinomas had some COX-2 protein expression. Expression of COX-2 protein was increased in IBD and colonic carcinomas compared with normal controls. There were no significant differences between colonic adenomas and colonic carcinomas. No correlation was found between COX-2 protein expression and patient gender, patient age, tumor size, tumor location or the degree of differentiation/ metastasis of the tumor. Strong immunoreactive COX-2 was expressed in clusters in interstitial cells (mainly mononuclear cells) in 53.6% (15/28) of adenomas and 64.3% (9/14) of colonic carcinomas. Strong COX-2 protein expression was also displayed in the normal glands that were adjacent to the adenomas and carcinomas. Expression of COX-1 protein was observed in the epithelial cells and interstitial cells or tumor cells of normal colon, IBD, colonic adenomas and colonic carcinomas. CONCLUSIONS: Our results indicated that COX-2 protein overexpression may contribute to the development of IBD and colonic carcinogenesis.
{"title":"Expression of cyclooxygenase‐2 protein in colon disease","authors":"Hong Lu, Z. Ge, Wenzhong Liu, Xiao-yu Chen, Yanshen Peng, S. Xiao","doi":"10.1046/J.1443-9573.2001.00046.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00046.X","url":null,"abstract":"OBJECTIVE: Several epidemiological studies have indicated that the long-term administration of non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the incidence of colorectal cancer. The best known action of NSAIDs is to block cyclooxygenase, the key enzyme required for the conversion of arachidonic acid to prostaglandins. Two cyclooxygenase isoforms have been identified and these are referred to as COX-1 and COX-2. Recent studies indicate that inducible COX-2 plays an important role in gastrointestinal inflammation and carcinogenesis. The present study was undertaken to investigate the expression and clinical implications of COX-2 and COX-1 in normal and diseased colons. \u0000 \u0000 \u0000 \u0000METHODS: COX-2 and COX-1 protein expression in specimens from normal controls and diseased colon tissues were examined semiquantitatively by using immunohistochemical methods. \u0000 \u0000 \u0000 \u0000RESULTS: By using immunohistochemical detection methods, low COX-2 protein expression in colonic epithelial cells was observed in 20.0% (2/10) of normal controls. Eighty percent (16/20) of specimens from inflammatory bowel disease (IBD) had a high COX-2 expression, 46.40% (13/28) of adenomas and 64.3% of (9/14) well-differentiated colonic carcinomas had some COX-2 protein expression. Expression of COX-2 protein was increased in IBD and colonic carcinomas compared with normal controls. There were no significant differences between colonic adenomas and colonic carcinomas. No correlation was found between COX-2 protein expression and patient gender, patient age, tumor size, tumor location or the degree of differentiation/ metastasis of the tumor. Strong immunoreactive COX-2 was expressed in clusters in interstitial cells (mainly mononuclear cells) in 53.6% (15/28) of adenomas and 64.3% (9/14) of colonic carcinomas. Strong COX-2 protein expression was also displayed in the normal glands that were adjacent to the adenomas and carcinomas. Expression of COX-1 protein was observed in the epithelial cells and interstitial cells or tumor cells of normal colon, IBD, colonic adenomas and colonic carcinomas. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Our results indicated that COX-2 protein overexpression may contribute to the development of IBD and colonic carcinogenesis.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85330694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-04-01DOI: 10.1046/J.1443-9573.2001.00037.X
Cai Jian-ting, Qian Ke-da, Lu Junshen
OBJECTIVE: To study the effect of K-ras antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu 8988s at different times after treatment. METHODS: Human pancreatic cancer cells (PaTu 8988s) in exponential growth stage were used at a cell concentration of 1 × 105/mL; 0.5 mL of the cell suspension was placed in each well of replicate 24-well culture plates in the presence of different concentrations (50 and 100 μg/mL) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out 24, 48 and 72 h after treatment. RESULTS: At 12, 24, 48 and 72 h after ASODN treatment, the following rates of inhibition were observed: for 50 μg/mL, 42.3, 66.6, 69.6 and 74.6%, respectively; for 100 μg/mL, 66.2, 91.4, 98.2 and 98.3%, respectively. CONCLUSION: The inhibitory effect of ASODN began at 12 h post-treatment and became more marked at 48–72 h. The higher the concentration of ASODN, the earlier the peak of inhibitory rate appears.
{"title":"Effect of K-ras antisense oligodeoxynucleotides on human pancreatic cancer cell line PaTu 8988s","authors":"Cai Jian-ting, Qian Ke-da, Lu Junshen","doi":"10.1046/J.1443-9573.2001.00037.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00037.X","url":null,"abstract":"OBJECTIVE: To study the effect of K-ras antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu 8988s at different times after treatment. \u0000 \u0000 \u0000 \u0000METHODS: Human pancreatic cancer cells (PaTu 8988s) in exponential growth stage were used at a cell concentration of 1 × 105/mL; 0.5 mL of the cell suspension was placed in each well of replicate 24-well culture plates in the presence of different concentrations (50 and 100 μg/mL) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out 24, 48 and 72 h after treatment. \u0000 \u0000 \u0000 \u0000RESULTS: At 12, 24, 48 and 72 h after ASODN treatment, the following rates of inhibition were observed: for 50 μg/mL, 42.3, 66.6, 69.6 and 74.6%, respectively; for 100 μg/mL, 66.2, 91.4, 98.2 and 98.3%, respectively. \u0000 \u0000 \u0000 \u0000CONCLUSION: The inhibitory effect of ASODN began at 12 h post-treatment and became more marked at 48–72 h. The higher the concentration of ASODN, the earlier the peak of inhibitory rate appears.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72550718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-04-01DOI: 10.1046/J.1443-9573.2001.00031.X
Lin Zhihui, Liu Houyu, Pan Xiuzhen
OBJECTIVE: To investigate the effect of a specific antisense oligodeoxynucleotide (ASODN) on the telomerase activity of the human gastric cancer cell line FGC85. METHODS: FGC85 cells were treated in vitro with ASODN complementary to the template region of the RNA component of human telomerase. A cationic liposomal transfection reagent was used as a delivery vector. Telomerase activity was detected by using the telomeric repeat amplification protocol/polymerase chain reaction/enzyme-linked immunosorbent assay (TRAP-PCR-ELISA) method. RESULTS: The telomerase activity of FGC85 cells was suppressed significantly by the liposome/ASODN treatment and the effect was related to the con-centration of liposome/ASODN and treatment time. CONCLUSIONS: Specific ASODN have an inhibitory effect on telomerase activity in human gastric cancer cell line FGC85. Specific ASODN could be used in research into gene therapy for cancer treatments and the exploration of carcinogenesis.
{"title":"Inhibitory effect of a liposome-mediated antisense oligodeoxynucleotide on telomerase activity in a human gastric cancer cell line","authors":"Lin Zhihui, Liu Houyu, Pan Xiuzhen","doi":"10.1046/J.1443-9573.2001.00031.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00031.X","url":null,"abstract":"OBJECTIVE: To investigate the effect of a specific antisense oligodeoxynucleotide (ASODN) on the telomerase activity of the human gastric cancer cell line FGC85. \u0000 \u0000 \u0000 \u0000METHODS: FGC85 cells were treated in vitro with ASODN complementary to the template region of the RNA component of human telomerase. A cationic liposomal transfection reagent was used as a delivery vector. Telomerase activity was detected by using the telomeric repeat amplification protocol/polymerase chain reaction/enzyme-linked immunosorbent assay (TRAP-PCR-ELISA) method. \u0000 \u0000 \u0000 \u0000RESULTS: The telomerase activity of FGC85 cells was suppressed significantly by the liposome/ASODN treatment and the effect was related to the con-centration of liposome/ASODN and treatment time. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Specific ASODN have an inhibitory effect on telomerase activity in human gastric cancer cell line FGC85. Specific ASODN could be used in research into gene therapy for cancer treatments and the exploration of carcinogenesis.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77304370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-04-01DOI: 10.1046/J.1443-9573.2001.00034.X
Sun Xu, Lu Wei, Zhao Dianhui, Gen Daoying, S. Tianzhen, Chen Xing-rong
OBJECTIVE: To investigate the computed tomography (CT) virtual colographic features of colonic polyps, colorectal cancer, diverticula, ulcerative colitis and other benign colonic lesions. Also, to assess the value of this method in the diagnosis of colorectal lesions. METHODS: Computed tomography colography was performed in 37 patients (26 male, 11 female) suffering from the following conditions: 20 colonic adenomas, six colon cancers, four diverticula, five ulcerative colitis and one each of melanosis coli and amyloidosis. The data from CT scanning were processed by computer with specific software and the colonic lesions were evaluated with 2- or 3-D images, depending on the individual software. RESULTS: Seventeen cases of colonic adenoma, six colon cancers, four diverticula and two cases of ulcerative colitis were detected by using CT colography. However, melanosis coli and amyloidosis of the colon were not detected. CONCLUSION: Computed tomography colography can detect all colonic polyps of 0.5 cm in diameter or larger, colon cancer, diverticula and some ulcerative colitis successfully. It is quick, minimally invasive and able to be tolerated well. It has the potential to become an effective radiological tool in diagnosing colonic lesions.
{"title":"Clinical application of computed tomography virtual colography","authors":"Sun Xu, Lu Wei, Zhao Dianhui, Gen Daoying, S. Tianzhen, Chen Xing-rong","doi":"10.1046/J.1443-9573.2001.00034.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00034.X","url":null,"abstract":"OBJECTIVE: To investigate the computed tomography (CT) virtual colographic features of colonic polyps, colorectal cancer, diverticula, ulcerative colitis and other benign colonic lesions. Also, to assess the value of this method in the diagnosis of colorectal lesions. \u0000 \u0000 \u0000 \u0000METHODS: Computed tomography colography was performed in 37 patients (26 male, 11 female) suffering from the following conditions: 20 colonic adenomas, six colon cancers, four diverticula, five ulcerative colitis and one each of melanosis coli and amyloidosis. The data from CT scanning were processed by computer with specific software and the colonic lesions were evaluated with 2- or 3-D images, depending on the individual software. \u0000 \u0000 \u0000 \u0000RESULTS: Seventeen cases of colonic adenoma, six colon cancers, four diverticula and two cases of ulcerative colitis were detected by using CT colography. However, melanosis coli and amyloidosis of the colon were not detected. \u0000 \u0000 \u0000 \u0000CONCLUSION: Computed tomography colography can detect all colonic polyps of 0.5 cm in diameter or larger, colon cancer, diverticula and some ulcerative colitis successfully. It is quick, minimally invasive and able to be tolerated well. It has the potential to become an effective radiological tool in diagnosing colonic lesions.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76031817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-04-01DOI: 10.1046/J.1443-9573.2001.00036.X
Ma Jie, Z. Xiaojun, Zhang Taihe, Sun Gui-qin, Meng Kui
OBJECTIVE: To observe changes in sinusoid lining endothelial cells (SEC), type IV collagen (CoIV) and laminin (LM) in chronic liver disease and hepatocellular carcinoma (HCC). To assess the clinicopathological significance of these changes in HCC. METHODS: Thirty specimens were taken from 30 cases of HCC (together with corresponding non-cancerous tissues), 10 cases of liver cirrhosis, five cases of mild chronic hepatitis and and four cases of normal liver tissues. The specimens were tested for CD34, CoIV and LM by using immunohistochemical methods. CD34, CoIV and LM were semiquantitatively analyzed and assessed in the context of the clinical and pathological features of HCC. RESULTS: CD34 and LM were not present along the sinusoidal walls in normal human liver, however, CoIV was weakly and discontinuously distributed along the sinusoidal walls. In cirrhosis, positive expression of CoIV increased significantly in the sinusoidal walls and became continuous and homogeneous. CD34 and LM were weakly present in the perinodules in a few cases of cirrhosis with obvious inflammatory infiltration. Hepatocellular carcinoma showed a diffuse capillarization, with overexpression of CD34, CoIV and LM. CoIV and LM expression were reduced in poorly differentiated HCC and HCC with portal vein thrombosis. This was frequently accompanied by breaks and losses in the basement membrane. The expression of CD34 in tumors of 5 cm in diameter. The expression of CD34 and LM was markedly increased in HCC compared with non-cancerous liver tissues. CONCLUSIONS: Diffuse capillarization with overexpression of CD34, CoIV and LM are features of HCC. Frequent breaks in, loss of and decrease of the basement membrane in poorly differentiated tumors and tumors with portal vein infiltration suggests potential metastasis of tumor cells and may play a major role in the metastasis of HCC. CD34 is a useful marker for distinguishing HCC from non-cancerous liver tissues.
{"title":"Pathological observations of sinusoid lining endothelial cells and the basement membrane in human hepatocellular carcinoma","authors":"Ma Jie, Z. Xiaojun, Zhang Taihe, Sun Gui-qin, Meng Kui","doi":"10.1046/J.1443-9573.2001.00036.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00036.X","url":null,"abstract":"OBJECTIVE: To observe changes in sinusoid lining endothelial cells (SEC), type IV collagen (CoIV) and laminin (LM) in chronic liver disease and hepatocellular carcinoma (HCC). To assess the clinicopathological significance of these changes in HCC. \u0000 \u0000 \u0000 \u0000METHODS: Thirty specimens were taken from 30 cases of HCC (together with corresponding non-cancerous tissues), 10 cases of liver cirrhosis, five cases of mild chronic hepatitis and and four cases of normal liver tissues. The specimens were tested for CD34, CoIV and LM by using immunohistochemical methods. CD34, CoIV and LM were semiquantitatively analyzed and assessed in the context of the clinical and pathological features of HCC. \u0000 \u0000 \u0000 \u0000RESULTS: CD34 and LM were not present along the sinusoidal walls in normal human liver, however, CoIV was weakly and discontinuously distributed along the sinusoidal walls. In cirrhosis, positive expression of CoIV increased significantly in the sinusoidal walls and became continuous and homogeneous. CD34 and LM were weakly present in the perinodules in a few cases of cirrhosis with obvious inflammatory infiltration. Hepatocellular carcinoma showed a diffuse capillarization, with overexpression of CD34, CoIV and LM. CoIV and LM expression were reduced in poorly differentiated HCC and HCC with portal vein thrombosis. This was frequently accompanied by breaks and losses in the basement membrane. The expression of CD34 in tumors of 5 cm in diameter. The expression of CD34 and LM was markedly increased in HCC compared with non-cancerous liver tissues. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Diffuse capillarization with overexpression of CD34, CoIV and LM are features of HCC. Frequent breaks in, loss of and decrease of the basement membrane in poorly differentiated tumors and tumors with portal vein infiltration suggests potential metastasis of tumor cells and may play a major role in the metastasis of HCC. CD34 is a useful marker for distinguishing HCC from non-cancerous liver tissues.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90592224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-04-01DOI: 10.1046/J.1443-9573.2001.00029.X
Zhang Shasha, Z. Jun, Luo Jinyan, Wang Kangming, Gong Jun, Zuo Aili
OBJECTIVE: The aim of the present study was to investigate the factors predisposing patients with Barrett’s esophagus (BE) to intestinal metaplasia (IM). METHODS: Forty-seven BE patients were studied. By using endoscopic and histological methods, esophageal IM was diagnosed in 36 patients, who were compared with 11 patients without IM in regard to their age, the endoscopic appearance of the esophagus and esophageal motility. RESULTS: The patients’ age and endoscopic features of the esophagus, but not esophageal motor function and grade of macroscopic esophagitis, were risk factors for the development of IM. CONCLUSIONS: Patient age and endoscopic features of the BE mucosa are associated with IM and may be prognostic factors for BE.
{"title":"Study of intestinal metaplasia in Barrett’s esophagus","authors":"Zhang Shasha, Z. Jun, Luo Jinyan, Wang Kangming, Gong Jun, Zuo Aili","doi":"10.1046/J.1443-9573.2001.00029.X","DOIUrl":"https://doi.org/10.1046/J.1443-9573.2001.00029.X","url":null,"abstract":"OBJECTIVE: The aim of the present study was to investigate the factors predisposing patients with Barrett’s esophagus (BE) to intestinal metaplasia (IM). \u0000 \u0000 \u0000 \u0000METHODS: Forty-seven BE patients were studied. By using endoscopic and histological methods, esophageal IM was diagnosed in 36 patients, who were compared with 11 patients without IM in regard to their age, the endoscopic appearance of the esophagus and esophageal motility. \u0000 \u0000 \u0000 \u0000RESULTS: The patients’ age and endoscopic features of the esophagus, but not esophageal motor function and grade of macroscopic esophagitis, were risk factors for the development of IM. \u0000 \u0000 \u0000 \u0000CONCLUSIONS: Patient age and endoscopic features of the BE mucosa are associated with IM and may be prognostic factors for BE.","PeriodicalId":10082,"journal":{"name":"Chinese journal of digestive diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78357584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：