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An improved no-moving-parts video-rate confocal microscope 一种改进的无移动部件视频速率共聚焦显微镜
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90019-A
Seth R. Goldstein , Thomas Hubin , Thomas G. Smith Jr.

Several years ago our research program developed a video-rate confocal microscope with no moving parts, based on synchronizing and aligning the scan of an image dissector tube (IDT) with the light returning from a microscope stage that has been acousto-optically scanned by a laser beam. Improvements on the original system have recently been completed. The laser power has been substantially increased and the laser scan is now brought into the Nikon Diaphot inverted microscope through the epi-illumination port. Aberrations in the scanned beam have been reduced by performing the beam shaping required by the acousto-optic deflectors using prisms instead of cylindrical lenses. The IDT is located at the side camera output port at the end of a simple, efficient light path. The new system is described in detail and results obtained using the microscope in reflection and fluorescence mode are presented.

几年前，我们的研究项目开发了一种无运动部件的视频速率共聚焦显微镜，其原理是将成像分解管(IDT)的扫描与经过激光束声光扫描的显微镜台返回的光同步并对齐。对原有系统的改进最近已经完成。激光功率已经大大增加，激光扫描现在被带入尼康透照倒置显微镜通过外延照明端口。通过使用棱镜代替圆柱透镜进行声光偏转器所需的光束整形，降低了扫描光束的像差。IDT位于简单、高效光路末端的侧相机输出端口。详细介绍了新系统，并给出了用显微镜在反射和荧光模式下得到的结果。
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引用次数: 6
Molecular biology in living cells by means of digital optical microscopy 用数码光学显微镜研究活细胞的分子生物学
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90028-C
Paolo Gualtieri

The intention of this review is to introduce to microscopists some of the fundamental limits as well as the advances made in digital optical microscopy techniques, also to show their potentialities in the field of molecular cell biology. Several items within the wide field of this subject will be discussed, such as geometric and photometric features, photophysics, statistics of photon flux and detection, spatial and intensity characteristics of digital images, their manipulation and display, fluorescent probes, and measuring techniques.

这篇综述的目的是向显微镜学家介绍数字光学显微镜技术的一些基本限制和取得的进展，并展示它们在分子细胞生物学领域的潜力。本课程将讨论本学科广泛领域内的几个项目，例如几何和光度特征、光物理学、光子通量和探测的统计、数字图像的空间和强度特征、它们的操作和显示、荧光探针和测量技术。
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引用次数: 16
Effect of tunicamycin on autophagy in cultured rat fibroblasts tunicamycin对培养大鼠成纤维细胞自噬的影响
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90097-W
Eeva-Liisa Punnonen, Soile Autio, Hilkka Reunanen
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引用次数: 0
A sensitive way to determine the thickness of thin carbon films by SEM 一种用扫描电镜测定碳薄膜厚度的灵敏方法
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90108-P
M.D. Bentzon , S.S. Eskildsen , P.S. Nielsen
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引用次数: 0
X-ray microanalytical studies on phytoplankton and suspended matter in a polluted freshwater environment: Lake Maryut, Alexandria, Egypt 埃及亚历山大Maryut湖污染淡水环境中浮游植物和悬浮物的x射线微量分析研究
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90044-E
E. El-Bestawy , E. Bellinger, M. Hassan, S. Helmi, D.C. Sigee
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引用次数: 2
Quantitative electronprobe X-ray microanalysis on the calcium and phosphorus distribution and calcium transport in epiphyseal growth plate 骨骺生长板中钙、磷分布及钙转运的定量电子探针x射线显微分析
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90047-H
Ernst-Rudolf Krefting, Kai Frentzel, Jörg Teβarek, Hans-Jürgen Hohling
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引用次数: 0
HREM study of zeolite Na-Y Na-Y沸石的HREM研究
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90104-L
Viveka Alfredsson
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引用次数: 2
Limitations on cell volume determination using three-dimensional optical microscopy 三维光学显微镜测定细胞体积的局限性
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90023-7
D.F. Abbott, K.A. Nugent

One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width.

三维显微镜的一个重要应用是测定生物细胞的体积。本文研究了三维反褶积技术在细胞体积测定中的应用。理论讨论是基于可靠的图像重建的傅里叶采样条件，并表明，使用该条件和模拟图像，细胞体积的确定是可靠的，只有细胞的形状和方向，使其沿光轴的长度远远大于其宽度。
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引用次数: 1
HREM investigations of two minerals, Cu3(As,Sb)S4 两种矿物Cu3(As,Sb)S4的HREM研究
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90141-Y
Mihály Pósfai , Margareta Sundberg
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引用次数: 0
Author checklist 作者检查表
Micron and Microscopica Acta
Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90158-A
{"title":"Author checklist","authors":"","doi":"10.1016/0739-6260(92)90158-A","DOIUrl":"https://doi.org/10.1016/0739-6260(92)90158-A","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Page II"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90158-A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91996157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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