Pub Date : 2020-08-11DOI: 10.3760/CMA.J.CN114452-20200423-00423
Wenjuan Wu, Min Li, Yiwei Tang, Dong Li, M. Guan
At present, the situation of COVID-19 epidemic prevention and control in China is gradually improving, but the situation of overseas import prevention and control remains difficult The COVID-19 epidemic may exist for long due to the undetermined source of infection, the difficulty in completely cutting off the transmission route, and a large number of susceptible people Therefore, prevention and control will be a long-term and arduous task, making it necessary to adhere to the principle of combining emergency response with regular prevention and control, coordinating the epidemic prevention and social-economic development in a balanced way In retrospect, the epidemic has exposed the ambiguous positioning and unsatisfying hardware construction of hospital laboratory departments, and delayed intervention of laboratory experts in the public health treatment system of China This paper reflects on the hospital laboratory departments' problems during the anti-epidemic activities, and put forward suggestions to improve the future development of clinical laboratories in the national public health system
{"title":"The position and role of department of laboratory medicine in the construction of national public health system: experiences from public health emergencies efforts","authors":"Wenjuan Wu, Min Li, Yiwei Tang, Dong Li, M. Guan","doi":"10.3760/CMA.J.CN114452-20200423-00423","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200423-00423","url":null,"abstract":"At present, the situation of COVID-19 epidemic prevention and control in China is gradually improving, but the situation of overseas import prevention and control remains difficult The COVID-19 epidemic may exist for long due to the undetermined source of infection, the difficulty in completely cutting off the transmission route, and a large number of susceptible people Therefore, prevention and control will be a long-term and arduous task, making it necessary to adhere to the principle of combining emergency response with regular prevention and control, coordinating the epidemic prevention and social-economic development in a balanced way In retrospect, the epidemic has exposed the ambiguous positioning and unsatisfying hardware construction of hospital laboratory departments, and delayed intervention of laboratory experts in the public health treatment system of China This paper reflects on the hospital laboratory departments' problems during the anti-epidemic activities, and put forward suggestions to improve the future development of clinical laboratories in the national public health system","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"7 19 1","pages":"776-779"},"PeriodicalIF":0.0,"publicationDate":"2020-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87293660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-11DOI: 10.3760/CMA.J.CN114452-20200304-00176
Xue Li, Jia Liu, Boan Li
Studies have confirmed that the outbreak was caused by a new type of coronavirus infection, which is highly contagious In this paper, the research progress of coronaviruses and 2019 novel coronavirus as well as the laboratory testing and related biosafety requirements of 2019 novel coronavirus are reviewed to provide relevant basis for the work of medical laboratories and testing institutions
{"title":"Advances in 2019 novel coronavirus research and laboratory testing","authors":"Xue Li, Jia Liu, Boan Li","doi":"10.3760/CMA.J.CN114452-20200304-00176","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200304-00176","url":null,"abstract":"Studies have confirmed that the outbreak was caused by a new type of coronavirus infection, which is highly contagious In this paper, the research progress of coronaviruses and 2019 novel coronavirus as well as the laboratory testing and related biosafety requirements of 2019 novel coronavirus are reviewed to provide relevant basis for the work of medical laboratories and testing institutions","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"728 1","pages":"368-372"},"PeriodicalIF":0.0,"publicationDate":"2020-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82323552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-03DOI: 10.3760/CMA.J.CN114452-20200318-00271
Rui Zhang, Jinming Li
Assays of specific immunoglobulin M (IgM) and immunoglobulin G (IgG) have been supplemented in "diagnosis and treatment guidelines of Novel Coronavirus pneumonia" (7th edition) issued by National Health Commission of the People’s Republic of China as an auxiliary evidence to diagnose Novel Coronavirus pneumonia. However, the false positive results is a major problem in the application of antibody assays. The factors causing the false positive results were analyzed and the ways were discussed to reduce the false positive results. The false positive results of antibody assays can be reduced, but they cannot be fully resolved. Antibody assays are valuable criteria for the diagnosis of suspected cases with negative nucleic acid results only if at least two dynamic tests are used. Key words: Coronavirus; Immunoglobulin M; Immunoglobulin G; False positive reactions
{"title":"Reasons and solutions for 'false positive results' of 2019 Novel Coronavirus-specific antibodies detection/ 中华检验医学杂志","authors":"Rui Zhang, Jinming Li","doi":"10.3760/CMA.J.CN114452-20200318-00271","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200318-00271","url":null,"abstract":"Assays of specific immunoglobulin M (IgM) and immunoglobulin G (IgG) have been supplemented in \"diagnosis and treatment guidelines of Novel Coronavirus pneumonia\" (7th edition) issued by National Health Commission of the People’s Republic of China as an auxiliary evidence to diagnose Novel Coronavirus pneumonia. However, the false positive results is a major problem in the application of antibody assays. The factors causing the false positive results were analyzed and the ways were discussed to reduce the false positive results. The false positive results of antibody assays can be reduced, but they cannot be fully resolved. Antibody assays are valuable criteria for the diagnosis of suspected cases with negative nucleic acid results only if at least two dynamic tests are used. \u0000 \u0000Key words: \u0000Coronavirus; Immunoglobulin M; Immunoglobulin G; False positive reactions","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83071835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-16DOI: 10.3760/CMA.J.CN114452-20200303-00165
Dongge Xia, Yanhua Du, Jun-yi Wu
Recently, Novel Coronavirus Pneumonia (NCP) was firstly reported and identified in Wuhan City, Hubei province, China. Therefore, it is valuable to establish an efficient and accurate virus detection strategy. Although the examination of viral nucleic acid maybe the gold standard for diagnosing NCP, there are still many problems in related research. Therefore, utilizing the latest research technologies in the fields of molecular biology, cell biology, immunology to detect the virus in different types of clinical specimens, supplemented by information such as medical imaging (CT), can help achieve early detection, isolation or treatment of patients and finally through the current crisis. Key words: Coronavirus; Pneumonia, viral; Nucleic acids; Specimen handling; Genome, viral; Genetic variation
{"title":"Biological characteristics of 2019 novel coronavirus and strategies for clinical specimen collection","authors":"Dongge Xia, Yanhua Du, Jun-yi Wu","doi":"10.3760/CMA.J.CN114452-20200303-00165","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200303-00165","url":null,"abstract":"Recently, Novel Coronavirus Pneumonia (NCP) was firstly reported and identified in Wuhan City, Hubei province, China. Therefore, it is valuable to establish an efficient and accurate virus detection strategy. Although the examination of viral nucleic acid maybe the gold standard for diagnosing NCP, there are still many problems in related research. Therefore, utilizing the latest research technologies in the fields of molecular biology, cell biology, immunology to detect the virus in different types of clinical specimens, supplemented by information such as medical imaging (CT), can help achieve early detection, isolation or treatment of patients and finally through the current crisis. \u0000 \u0000Key words: \u0000Coronavirus; Pneumonia, viral; Nucleic acids; Specimen handling; Genome, viral; Genetic variation","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"109 1","pages":"931-934"},"PeriodicalIF":0.0,"publicationDate":"2020-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79304641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-11DOI: 10.3760/CMA.J.ISSN.1009-9158.2020.03.026
Guotao Lu, Mei Wei, Wei-qin Li
In recent years,with the change of lifestyle in China, hypertriglyceridemia (HTG) has been the second leading cause of acute pancreatitis (AP). Recent studies have shown that HTG has long-lasting influences on AP onset (including recurrence), AP severity, as well as pancreatic regeneration. However, the underlying mechanisms of HTG-AP is still unclear, mainly focusing on free fatty acid, microcirculation disturbance, calcium overload, oxidative stress, gene polymorphism and so on. However, there is lack of effective intervention for the diagnosis and treatment of HTG-AP, which needs further study. Key words: Hypertriglyceridemia; Acute pancreatitis; Research advances
{"title":"Research advances of hypertriglyceridemia acute pancreatitis","authors":"Guotao Lu, Mei Wei, Wei-qin Li","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.026","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.026","url":null,"abstract":"In recent years,with the change of lifestyle in China, hypertriglyceridemia (HTG) has been the second leading cause of acute pancreatitis (AP). Recent studies have shown that HTG has long-lasting influences on AP onset (including recurrence), AP severity, as well as pancreatic regeneration. However, the underlying mechanisms of HTG-AP is still unclear, mainly focusing on free fatty acid, microcirculation disturbance, calcium overload, oxidative stress, gene polymorphism and so on. However, there is lack of effective intervention for the diagnosis and treatment of HTG-AP, which needs further study. \u0000 \u0000 \u0000Key words: \u0000Hypertriglyceridemia; Acute pancreatitis; Research advances","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"10 1","pages":"336-340"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74716810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To evaluate the ability of Sysmex urine automatic analyzer UF-5000 to detect renal tubular epithelial cells, and to explore the value of detection of renal tubular epithelial cells in renal tubular injury of diabetes mellitus. Methods Case control study. 452 urine samples were collected from the third Xiangya Third Hospital of Central South University from October 2018 to April 2019 (252 in the control group, 113 in diabetes without renal injury group and 87 in diabetes with renal injury group). All samples were detected by both UF-5000 and microscopic examination, established reference range for normal population, then contrasted the coincidence rate and uniformity of the two methods, to evaluate the ability of urine automatic analyzer UF-5000 to detect renal tubular epithelial cells, and the diagnostic value of tubular epithelial cells for renal tubular injury in diabetic patients. All statistical analyses were performed using SPSS17.0, Kappa consistency analysis, ROC curve analysis, Kruskal-Wallis test and Chi-square test were used. Results The reference range of renal tubular epithelial cells by Sysmex urine automatic analyzer UF-5000 is 0-1.7/μl. The results of the two methods were analyzed by Kappa consistency analysis. The Kappa value was 0.699, P>0.05, which meant highly consistent. ROC curve analysis showed when cut-off value was 1.7/μl. The sensitivity, specificity and area under ROC curve were 0.791, 0.817 and 0.861 respectively. The median of renal tubular epithelial cells was 0.4/μl, 2.0/μl and 2.3/μl in the healthy control group, the diabetes without renal injury group and the diabetes with renal injury group, respectively; the positive rate of renal tubular epithelial cells in the three groups were 2.78%, 56.64% and 75.86% respectively. Compared with the control group, the median and positive rate of renal tubular epithelial cells in the diabetes without renal injury group and the diabetes with renal injury group were significant different; there was also significant difference in the positive rate of renal tubular epithelial cells between the two groups. Conclusion Compared with the control group, the positive rate of urine renal tubular epithelial cells indiabetes without renal injury group is significantly higher, which is helpful to detect renal tubular injury, to carry out early intervention and to prolong the time of progression to chronic kidney disease. Key words: Renal tubular epithelial cells; Diabetes; Renal tubular injury
{"title":"Application of urinary tubular epithelial cells in the renal tubular injury in diabetes mellitus","authors":"Jinling Yuan, Yong Wu, Yu-jue Cao, Yingjia Li, Zhu-ming Guan, Yu-hao Zhu, J. Liao","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.023","url":null,"abstract":"Objective \u0000To evaluate the ability of Sysmex urine automatic analyzer UF-5000 to detect renal tubular epithelial cells, and to explore the value of detection of renal tubular epithelial cells in renal tubular injury of diabetes mellitus. \u0000 \u0000 \u0000Methods \u0000Case control study. 452 urine samples were collected from the third Xiangya Third Hospital of Central South University from October 2018 to April 2019 (252 in the control group, 113 in diabetes without renal injury group and 87 in diabetes with renal injury group). All samples were detected by both UF-5000 and microscopic examination, established reference range for normal population, then contrasted the coincidence rate and uniformity of the two methods, to evaluate the ability of urine automatic analyzer UF-5000 to detect renal tubular epithelial cells, and the diagnostic value of tubular epithelial cells for renal tubular injury in diabetic patients. All statistical analyses were performed using SPSS17.0, Kappa consistency analysis, ROC curve analysis, Kruskal-Wallis test and Chi-square test were used. \u0000 \u0000 \u0000Results \u0000The reference range of renal tubular epithelial cells by Sysmex urine automatic analyzer UF-5000 is 0-1.7/μl. The results of the two methods were analyzed by Kappa consistency analysis. The Kappa value was 0.699, P>0.05, which meant highly consistent. ROC curve analysis showed when cut-off value was 1.7/μl. The sensitivity, specificity and area under ROC curve were 0.791, 0.817 and 0.861 respectively. The median of renal tubular epithelial cells was 0.4/μl, 2.0/μl and 2.3/μl in the healthy control group, the diabetes without renal injury group and the diabetes with renal injury group, respectively; the positive rate of renal tubular epithelial cells in the three groups were 2.78%, 56.64% and 75.86% respectively. Compared with the control group, the median and positive rate of renal tubular epithelial cells in the diabetes without renal injury group and the diabetes with renal injury group were significant different; there was also significant difference in the positive rate of renal tubular epithelial cells between the two groups. \u0000 \u0000 \u0000Conclusion \u0000Compared with the control group, the positive rate of urine renal tubular epithelial cells indiabetes without renal injury group is significantly higher, which is helpful to detect renal tubular injury, to carry out early intervention and to prolong the time of progression to chronic kidney disease. \u0000 \u0000 \u0000Key words: \u0000Renal tubular epithelial cells; Diabetes; Renal tubular injury","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"108 1","pages":"317-321"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87646566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-11DOI: 10.3760/CMA.J.ISSN.1009-9158.2020.03.021
Wen Shi, Jieru Huang, Dong-dong Liu, M. He, Chu-Wei Zheng, Qian Xiao, Li Lin, Yile Huang, Lin Li, Jianhua Xu
Objective To investigate the interfering factors in the determination of creatinine(Cr) using the American Clinical Laboratory Standards Association (CLSI) EP7-A3 document. Methods According to the CLSI EP7-A3 document, fresh serum (no hemolysis, lipemia, and jaundice) was used on the day of the experiment and confirmed the interfering substances through the pairing difference experiment and the point-to-point analysis method was used in the dose effect experiment to clarify the difference of interfering substances. Results Triglyceride (16.94 mmol/L), dobutamine hydrochloride (4.01 μmol/L), ascorbic acid (298 μmol/L) did not interfere with the determination of Cr. Free bilirubin (684 μmol/L), conjugated bilirubin (684 μmol/L), calcium hydroxybenzene sulfonate (144 μmol/L) and hemoglobin (10 g/L) were used as the maximum concentrations of interferences for the dose effect test, the results showed that the above interferences had negative interference on the determination of Cr. Conclusion According to EP7-A3, it is valuable to evaluate the interference factors of creatinine determination. Key words: Interference experiment; Creatinine
{"title":"Evaluation of interference factors in creatinine measurement by using new guideline CLSI EP7-A3","authors":"Wen Shi, Jieru Huang, Dong-dong Liu, M. He, Chu-Wei Zheng, Qian Xiao, Li Lin, Yile Huang, Lin Li, Jianhua Xu","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.021","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.021","url":null,"abstract":"Objective \u0000To investigate the interfering factors in the determination of creatinine(Cr) using the American Clinical Laboratory Standards Association (CLSI) EP7-A3 document. \u0000 \u0000 \u0000Methods \u0000According to the CLSI EP7-A3 document, fresh serum (no hemolysis, lipemia, and jaundice) was used on the day of the experiment and confirmed the interfering substances through the pairing difference experiment and the point-to-point analysis method was used in the dose effect experiment to clarify the difference of interfering substances. \u0000 \u0000 \u0000Results \u0000Triglyceride (16.94 mmol/L), dobutamine hydrochloride (4.01 μmol/L), ascorbic acid (298 μmol/L) did not interfere with the determination of Cr. Free bilirubin (684 μmol/L), conjugated bilirubin (684 μmol/L), calcium hydroxybenzene sulfonate (144 μmol/L) and hemoglobin (10 g/L) were used as the maximum concentrations of interferences for the dose effect test, the results showed that the above interferences had negative interference on the determination of Cr. \u0000 \u0000 \u0000Conclusion \u0000According to EP7-A3, it is valuable to evaluate the interference factors of creatinine determination. \u0000 \u0000 \u0000Key words: \u0000Interference experiment; Creatinine","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"67 16 1","pages":"307-311"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82014646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-11DOI: 10.3760/CMA.J.ISSN.1009-9158.2020.03.025
Wenbo Zhu, Qin-Rong Xu, Jingjing Li, Kui Zhang, B. Bai
With the expansion of aging population, Alzheimer′s disease (AD) is increasingly imposing a substantial burden on the society. Because of irreversible neuronal death in the brain, effective control of AD relies on early diagnosis for timely therapeutic intervention, and this requires efficient laboratory tests and molecular imaging. Current laboratory tests for AD includes measurements of Aβ peptides, Tau, and a number of other recently discovered molecules in the cerebrospinal fluid and the peripheral blood. PET-based imaging of glucose metabolism, amyloid Aβ, Tau neurofibrillary tangles, TSPO protein, and neuronal receptors, has also proven to be useful in clinical finding of AD. This review will focus on the progress in studies of these biomarkers and methodologies for ADdiagnosis. Key words: Alzheimer′s disease; Dementia; Positron-emission tomography
{"title":"Progress of clinical laboratory tests and molecular imaging for diagnosis of Alzheimer's disease","authors":"Wenbo Zhu, Qin-Rong Xu, Jingjing Li, Kui Zhang, B. Bai","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.025","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.025","url":null,"abstract":"With the expansion of aging population, Alzheimer′s disease (AD) is increasingly imposing a substantial burden on the society. Because of irreversible neuronal death in the brain, effective control of AD relies on early diagnosis for timely therapeutic intervention, and this requires efficient laboratory tests and molecular imaging. Current laboratory tests for AD includes measurements of Aβ peptides, Tau, and a number of other recently discovered molecules in the cerebrospinal fluid and the peripheral blood. PET-based imaging of glucose metabolism, amyloid Aβ, Tau neurofibrillary tangles, TSPO protein, and neuronal receptors, has also proven to be useful in clinical finding of AD. This review will focus on the progress in studies of these biomarkers and methodologies for ADdiagnosis. \u0000 \u0000 \u0000Key words: \u0000Alzheimer′s disease; Dementia; Positron-emission tomography","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"50 1","pages":"328-335"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85365483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-11DOI: 10.3760/CMA.J.ISSN.1009-9158.2020.03.013
Wen-jun Ma, J. Bian, Y. Lou, Xu Yang, Hui‐min Zhang, Xianliang Zhou, L. Song, Jun Cai, Beibei Zhao, Ergang Jiang, Li Weixia, Yating Cheng
Objective To explore the clinical utility of liquid chromatography tandem mass spectrometry forprimary aldosteronism screening. Methods From January to October 2019, 413 inpatients diagnosed hypertension from Fuwai Hospital of Chinese Academy of Medical Sciences were enrolled, including 60 Primary aldosteronism(PA)patients and 353 primary hypertension patients. The plasma aldosterone concentration (PAC) and renin concentration (DRC) were measured after 2 h of standing. The 24 h urine samples were collected for measurement of aldosterone using LC-MS/MS. The performance of urine aldosterone and urine aldosterone/renin ratio (UADRR) in PA screening was evaluated by ROC, and compared with PAC/DRC ratio (ADRR). Meanwhile, the efficiency of urine aldosterone in elderly patients or patients with low blood potassium or 24 h urine sodium over 200 mmol was investigated. Results Area under the curve (AUC)of urine aldosterone was 0.725 (95%CI 0.679-0.767), and the best cut-off was 7.13 μg/24 h, which was lower than AUC of ADRR (0.958, 95%CI 0.934-0.975). The AUC of UADRR was 0.947 (95%CI 0.920-0.966), the best cut-off was 1.11 (μg/24 h)/(μIU/ml), the sensitivity and specificity were 91.7% and 89.0%, respectively. There is no significant differences found with ADRR. In patients with 24 h urine sodium over 200 mmol, AUC of aldosterone was 0.834 (95%CI 0.730-0.910) and the best cut-off was 9.31 μg/24 h. The sensitivity and specificity were 90.9% and 68.7%, respectively. For the elderly patients over 60 years old, the AUC of urinary aldosterone was 0.860 (95%CI 0.770-0.925), and the best cut-off was 6.91 μg/24 h. The sensitivity and specificity were 84.6% and 81.3%, respectively. When admission blood potassium was less than 3.50 mmol/L, AUC of urinary aldosterone was 0.822 (95%CI 0.684-0.917), and the best cut-off was 10.63 μg/24 h. The sensitivity and specificity were 85.7% and 66.7%, respectively. Conclusion The detection of aldosterone in urine by LC-MS/MS can provide clinical information for PA screening, and the screening performance is better in patients with 24-hour urine sodium over 200 mmol, elderly patients or patients with low blood potassium. If combined with renin, screening efficiency was the same as that in ADRR. Key words: Hyperaldosteronism; Aldosterone; Urinalysis; Tandem mass spectrometry; Chromatography, liquid
{"title":"Clinical utility value of urinary aldosterone detection by tandem mass spectrometry in primary hyperaldosteronism screening","authors":"Wen-jun Ma, J. Bian, Y. Lou, Xu Yang, Hui‐min Zhang, Xianliang Zhou, L. Song, Jun Cai, Beibei Zhao, Ergang Jiang, Li Weixia, Yating Cheng","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.013","url":null,"abstract":"Objective \u0000To explore the clinical utility of liquid chromatography tandem mass spectrometry forprimary aldosteronism screening. \u0000 \u0000 \u0000Methods \u0000From January to October 2019, 413 inpatients diagnosed hypertension from Fuwai Hospital of Chinese Academy of Medical Sciences were enrolled, including 60 Primary aldosteronism(PA)patients and 353 primary hypertension patients. The plasma aldosterone concentration (PAC) and renin concentration (DRC) were measured after 2 h of standing. The 24 h urine samples were collected for measurement of aldosterone using LC-MS/MS. The performance of urine aldosterone and urine aldosterone/renin ratio (UADRR) in PA screening was evaluated by ROC, and compared with PAC/DRC ratio (ADRR). Meanwhile, the efficiency of urine aldosterone in elderly patients or patients with low blood potassium or 24 h urine sodium over 200 mmol was investigated. \u0000 \u0000 \u0000Results \u0000Area under the curve (AUC)of urine aldosterone was 0.725 (95%CI 0.679-0.767), and the best cut-off was 7.13 μg/24 h, which was lower than AUC of ADRR (0.958, 95%CI 0.934-0.975). The AUC of UADRR was 0.947 (95%CI 0.920-0.966), the best cut-off was 1.11 (μg/24 h)/(μIU/ml), the sensitivity and specificity were 91.7% and 89.0%, respectively. There is no significant differences found with ADRR. In patients with 24 h urine sodium over 200 mmol, AUC of aldosterone was 0.834 (95%CI 0.730-0.910) and the best cut-off was 9.31 μg/24 h. The sensitivity and specificity were 90.9% and 68.7%, respectively. For the elderly patients over 60 years old, the AUC of urinary aldosterone was 0.860 (95%CI 0.770-0.925), and the best cut-off was 6.91 μg/24 h. The sensitivity and specificity were 84.6% and 81.3%, respectively. When admission blood potassium was less than 3.50 mmol/L, AUC of urinary aldosterone was 0.822 (95%CI 0.684-0.917), and the best cut-off was 10.63 μg/24 h. The sensitivity and specificity were 85.7% and 66.7%, respectively. \u0000 \u0000 \u0000Conclusion \u0000The detection of aldosterone in urine by LC-MS/MS can provide clinical information for PA screening, and the screening performance is better in patients with 24-hour urine sodium over 200 mmol, elderly patients or patients with low blood potassium. If combined with renin, screening efficiency was the same as that in ADRR. \u0000 \u0000 \u0000Key words: \u0000Hyperaldosteronism; Aldosterone; Urinalysis; Tandem mass spectrometry; Chromatography, liquid","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"30 1","pages":"261-266"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76308881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-11DOI: 10.3760/CMA.J.ISSN.1009-9158.2020.03.002
Y. Tong, Ming Wang, Wan-zhou Xu, B. Qiao, Hongyun Zheng, Si-qing Mei, Xiaoyun He, Pingan Zhang, Yan Li
In December, the outbreak of a novel coronavirus (2019-nCoV) in Wuhan, China, has attracted extensive global attention. On January 20, 2020, the Chinese health authorities upgraded the coronavirus to a Class B infectious disease in the Law of the People′s Republic of China on the Prevention and Treatment of Infectious Diseases, and considered it as Class A infectious diseases in disease control and prevention. On January 18, 2020, the 2019-nCoV nucleic acid detection test was listed as the diagnostic criteria in the "guidelines for diagnosis and treatment of pneumonia due to 2019-nCoV (Trial Version 2)" . Therefore, standardizing the operation process of the 2019-nCoV nucleic acid detection in clinical laboratories has become a top priority. It is of paramount importance to establish standard protocols for detection of the 2019-nCoV nucleic acids in clinical laboratories to improve the reliability of the results and ensure the biosafety of laboratory personnel. Key words: Coronavirus; RNA, viral; Polymerase chain reaction; Laboratories, hospital; Medical laboratory science; Benchmarking; Containment of biohazards
{"title":"Proposal for standardization of 2019-nCoV nucleic acid detection in clinical laboratories","authors":"Y. Tong, Ming Wang, Wan-zhou Xu, B. Qiao, Hongyun Zheng, Si-qing Mei, Xiaoyun He, Pingan Zhang, Yan Li","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.002","url":null,"abstract":"In December, the outbreak of a novel coronavirus (2019-nCoV) in Wuhan, China, has attracted extensive global attention. On January 20, 2020, the Chinese health authorities upgraded the coronavirus to a Class B infectious disease in the Law of the People′s Republic of China on the Prevention and Treatment of Infectious Diseases, and considered it as Class A infectious diseases in disease control and prevention. On January 18, 2020, the 2019-nCoV nucleic acid detection test was listed as the diagnostic criteria in the \"guidelines for diagnosis and treatment of pneumonia due to 2019-nCoV (Trial Version 2)\" . Therefore, standardizing the operation process of the 2019-nCoV nucleic acid detection in clinical laboratories has become a top priority. It is of paramount importance to establish standard protocols for detection of the 2019-nCoV nucleic acids in clinical laboratories to improve the reliability of the results and ensure the biosafety of laboratory personnel. \u0000 \u0000 \u0000Key words: \u0000Coronavirus; RNA, viral; Polymerase chain reaction; Laboratories, hospital; Medical laboratory science; Benchmarking; Containment of biohazards","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"92 1","pages":"209-212"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77463025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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