Pub Date : 2021-01-01DOI: 10.3760/cma.j.cn114452-20200425-00429
M. Tong, C. Fang, H. Ge, S. Chen, H. Sheng
Laboratory diagnostics is a booming medical applied subjects. With the continuous enrichment of the content and the enhancement of its role in diagnosis, it has increasingly become a compulsory course for all medical students. Because the subject has many individual theoretical disciplines and application technologies, the traditional on-site teaching mode has been greatly challenged. Especially under the background of the pandemic, it is more difficult for the teaching by the online course totally. For this reason, the measures of key sessions,such as through preparation and peer review before the class, online question and answer between teachers and students, reviewing homework after the class and recording video for probation purpose were summarized and reviewed. The online course mode was supposed to realize "non-stop teaching during pandemic", but also realize the double guarantee of teaching plan and teaching effect, which provide experience for expanding the whole network course of laboratory diagnostics.
{"title":"Probes of whole network course of laboratory diagnostics under the background of pandemic situation","authors":"M. Tong, C. Fang, H. Ge, S. Chen, H. Sheng","doi":"10.3760/cma.j.cn114452-20200425-00429","DOIUrl":"https://doi.org/10.3760/cma.j.cn114452-20200425-00429","url":null,"abstract":"Laboratory diagnostics is a booming medical applied subjects. With the continuous enrichment of the content and the enhancement of its role in diagnosis, it has increasingly become a compulsory course for all medical students. Because the subject has many individual theoretical disciplines and application technologies, the traditional on-site teaching mode has been greatly challenged. Especially under the background of the pandemic, it is more difficult for the teaching by the online course totally. For this reason, the measures of key sessions,such as through preparation and peer review before the class, online question and answer between teachers and students, reviewing homework after the class and recording video for probation purpose were summarized and reviewed. The online course mode was supposed to realize \"non-stop teaching during pandemic\", but also realize the double guarantee of teaching plan and teaching effect, which provide experience for expanding the whole network course of laboratory diagnostics.","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"7 1","pages":"75-78"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81916113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.3760/cma.j.cn114452-20201120-00847
J. Hu, Yu Chen, Y. Zhou, Hang Yang
Immune disorders are currently recognized as the major cause of aggravation of clinical symptoms in corona virus disease 2019 (COVID-19) patients. This review summarized the characteristics of the immune response to COVID-19 from the abnormal dynamics of lymphocyte subsets, including the decrease in the count of natural killer cell and T cell subsets and the large accumulation of cytokines during disease progression. This review also clarified the possible mechanisms of changes in key lymphocyte subsets and the dynamic of cytokines. Thus, it provided new strategies for clinical treatment and granted potential targets for drug discovery.
{"title":"COVID-19: immune response and its implications for disease monitoring and therapy","authors":"J. Hu, Yu Chen, Y. Zhou, Hang Yang","doi":"10.3760/cma.j.cn114452-20201120-00847","DOIUrl":"https://doi.org/10.3760/cma.j.cn114452-20201120-00847","url":null,"abstract":"Immune disorders are currently recognized as the major cause of aggravation of clinical symptoms in corona virus disease 2019 (COVID-19) patients. This review summarized the characteristics of the immune response to COVID-19 from the abnormal dynamics of lymphocyte subsets, including the decrease in the count of natural killer cell and T cell subsets and the large accumulation of cytokines during disease progression. This review also clarified the possible mechanisms of changes in key lymphocyte subsets and the dynamic of cytokines. Thus, it provided new strategies for clinical treatment and granted potential targets for drug discovery.","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"66 1","pages":"873-876"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77961678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.3760/cma.j.cn114452-20210425-00266
J. Li, Q. Wang
In order to achieve fast turnaround time, reduce the instruments and workplace requirement, newly developed point-of-care nucleic acid testing for 2019 novel coronavirus (2019-nCoV) is increasingly used for the purpose of prevention and control of COVID-19. Since the methods used now have not met the requirements for point-of-care testing (POCT), these methods are temporarily named as "rapid nucleic acid testing" in this consensus. To standardize the clinical application of rapid nucleic acid testing of 2019-nCoV, experts from China Association of Medical Equipment of Genetic Testing, China Association of Medical Equipment of Point of Care Testing (POCT), National Clinical Research Center for Clinical Laboratory, Molecular Group of Laboratory Medicine Expert Committee of National Health Commission Capacity Building and Continuing Education Center, Beijing Medical Laboratory Quality Control and Improvement Center jointly developed this expert consensus, which provides the consensus recommendations for "rapid nucleic acid testing" in clinical laboratories, including general laboratory requirements, specimen collection, transportation and storage, standard operating procedures, verification, internal quality control, external quality assessment, result analysis and reporting, as well as laboratory safety management.
{"title":"Chinese expert consensus on the rapid nucleic acid testing of 2019-nCoV","authors":"J. Li, Q. Wang","doi":"10.3760/cma.j.cn114452-20210425-00266","DOIUrl":"https://doi.org/10.3760/cma.j.cn114452-20210425-00266","url":null,"abstract":"In order to achieve fast turnaround time, reduce the instruments and workplace requirement, newly developed point-of-care nucleic acid testing for 2019 novel coronavirus (2019-nCoV) is increasingly used for the purpose of prevention and control of COVID-19. Since the methods used now have not met the requirements for point-of-care testing (POCT), these methods are temporarily named as \"rapid nucleic acid testing\" in this consensus. To standardize the clinical application of rapid nucleic acid testing of 2019-nCoV, experts from China Association of Medical Equipment of Genetic Testing, China Association of Medical Equipment of Point of Care Testing (POCT), National Clinical Research Center for Clinical Laboratory, Molecular Group of Laboratory Medicine Expert Committee of National Health Commission Capacity Building and Continuing Education Center, Beijing Medical Laboratory Quality Control and Improvement Center jointly developed this expert consensus, which provides the consensus recommendations for \"rapid nucleic acid testing\" in clinical laboratories, including general laboratory requirements, specimen collection, transportation and storage, standard operating procedures, verification, internal quality control, external quality assessment, result analysis and reporting, as well as laboratory safety management.","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"22 1","pages":"698-702"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75260588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.3760/cma.j.cn114452-20201229-00933
H. Yu, F. Ren, Y. Zhang
Objective: To select nucleic acid extraction reagents and amplification reagents by comparing the minimum detection limit, amplification efficiency, specificity, accuracy, and matching analysis with 6 nucleic acid extraction reagents of 6 novel coronavirus (2019-nCoV) nucleic acid detection reagents and provide scientific basis for clinical diagnosis and treatment. Methods: The national-standard material of 2019-nCoV nuclear acids was diluted as ths six fradient concentrations. Then, the diluted samples were analyzed by using the six kinds of agents/kits, Guangzhou Daan (DA), Beijing Jinhao (JH), Wuhan Mingde (MD), Sichuan Mike (MK), Jiangsu Shuoshi (SS) and Shanghai Zhijiang (ZJ). The minimum detection limit, amplification efficiency, accuracy, specificity and other properties of these kits were examined. The positive control levels 1, 2 (low and medium concentration) were extracted by using the six nucleic acid extraction reagents (numbered a-f) with six 2019-nCoV detective kits to detect target genes of 2019-nCoV genome, and compared between different nucleic acid extraction reagents and different nucleic acid detection reagents. Match between the different nucleic acid extraction reagents detection reagents, using two-way analysis of variance. Results: The minimum detection limit of Nucleocapsid (N) gene of DA, JH, MD, SS reagent was 7.0×102 copies/ml.The minimum detection limit of open reading frame 1ab (ORF1ab) gene of JH, MD, SS, MK reagent was 9.39 ×102 copies/ml.The minimum detection limit of envelope (E) gene of MK, ZJ reagent was 5.03×102 copies/ml. SS reagent N gene amplification efficiency was 89%, ORF1ab gene amplification efficiency was 90%, was the highest among six reagents.a, b, c, d extraction reagents and 6 nucleic acid detection reagents match well, But a extraction reagent was more suitable for use with SS,MK and ZJ reagents (P<0.05), d extraction reagent was more suitable for MD,SS,DA,JH and MK nucleic acid detection reagents(P<0.05);e extraction reagent was more suitable for SS nucleic acid detection reagents(P<0.05), f extraction reagent was most suitable for DA, JH Nucleic acid detection reagents (P<0.05);e and f reagents were not suitable for use with MK and ZJ. Conclusion: The 6 detection reagents have good performance, and the appropriate extraction reagents and detection reagents should be used in conjunction with the actual conditions of the laboratory.
{"title":"Performance evaluation of six coronavirus nucleic acid detection reagents and matching analysis with nucleic acid extraction reagents","authors":"H. Yu, F. Ren, Y. Zhang","doi":"10.3760/cma.j.cn114452-20201229-00933","DOIUrl":"https://doi.org/10.3760/cma.j.cn114452-20201229-00933","url":null,"abstract":"Objective: To select nucleic acid extraction reagents and amplification reagents by comparing the minimum detection limit, amplification efficiency, specificity, accuracy, and matching analysis with 6 nucleic acid extraction reagents of 6 novel coronavirus (2019-nCoV) nucleic acid detection reagents and provide scientific basis for clinical diagnosis and treatment. Methods: The national-standard material of 2019-nCoV nuclear acids was diluted as ths six fradient concentrations. Then, the diluted samples were analyzed by using the six kinds of agents/kits, Guangzhou Daan (DA), Beijing Jinhao (JH), Wuhan Mingde (MD), Sichuan Mike (MK), Jiangsu Shuoshi (SS) and Shanghai Zhijiang (ZJ). The minimum detection limit, amplification efficiency, accuracy, specificity and other properties of these kits were examined. The positive control levels 1, 2 (low and medium concentration) were extracted by using the six nucleic acid extraction reagents (numbered a-f) with six 2019-nCoV detective kits to detect target genes of 2019-nCoV genome, and compared between different nucleic acid extraction reagents and different nucleic acid detection reagents. Match between the different nucleic acid extraction reagents detection reagents, using two-way analysis of variance. Results: The minimum detection limit of Nucleocapsid (N) gene of DA, JH, MD, SS reagent was 7.0×102 copies/ml.The minimum detection limit of open reading frame 1ab (ORF1ab) gene of JH, MD, SS, MK reagent was 9.39 ×102 copies/ml.The minimum detection limit of envelope (E) gene of MK, ZJ reagent was 5.03×102 copies/ml. SS reagent N gene amplification efficiency was 89%, ORF1ab gene amplification efficiency was 90%, was the highest among six reagents.a, b, c, d extraction reagents and 6 nucleic acid detection reagents match well, But a extraction reagent was more suitable for use with SS,MK and ZJ reagents (P<0.05), d extraction reagent was more suitable for MD,SS,DA,JH and MK nucleic acid detection reagents(P<0.05);e extraction reagent was more suitable for SS nucleic acid detection reagents(P<0.05), f extraction reagent was most suitable for DA, JH Nucleic acid detection reagents (P<0.05);e and f reagents were not suitable for use with MK and ZJ. Conclusion: The 6 detection reagents have good performance, and the appropriate extraction reagents and detection reagents should be used in conjunction with the actual conditions of the laboratory.","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"215 1","pages":"841-848"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79576490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.3760/cma.j.cn114452-20201015-00775
B. Zheng, Y. Tang, Yirong Li
Since the outbreak of the novel coronavirus pneumonia (COVID-19), it is urgently to develop the effective strategies for its clinical test or treatments world widely. Extracellular vesicles (EV)/exosomes could function as effective carriers for intercellular communication. Increasing evidence revealed that mesenchymal stem cell-derived EV/exosomes could be considered as an alternative cell-free therapy against the acute respiratory distress syndrome. Moreover, the EV-related metabolomics, proteomics, relapsing, deep-vein-thrombosis, myocardial-toxicity, liquid-biopsy and bio-therapeutic researches focusing on the COVID-19 will not only extended our knowledge for the diagnosis, but also provide novel ideas for treatment of this fatal disease. This article will systemically review the progresses of EV/exosomes in the diagnosis and treatment of COVID-19.
{"title":"Potential value of extracellular vesicles/exosomes in diagnosis and treatment of COVID-19","authors":"B. Zheng, Y. Tang, Yirong Li","doi":"10.3760/cma.j.cn114452-20201015-00775","DOIUrl":"https://doi.org/10.3760/cma.j.cn114452-20201015-00775","url":null,"abstract":"Since the outbreak of the novel coronavirus pneumonia (COVID-19), it is urgently to develop the effective strategies for its clinical test or treatments world widely. Extracellular vesicles (EV)/exosomes could function as effective carriers for intercellular communication. Increasing evidence revealed that mesenchymal stem cell-derived EV/exosomes could be considered as an alternative cell-free therapy against the acute respiratory distress syndrome. Moreover, the EV-related metabolomics, proteomics, relapsing, deep-vein-thrombosis, myocardial-toxicity, liquid-biopsy and bio-therapeutic researches focusing on the COVID-19 will not only extended our knowledge for the diagnosis, but also provide novel ideas for treatment of this fatal disease. This article will systemically review the progresses of EV/exosomes in the diagnosis and treatment of COVID-19.","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"30 1","pages":"537-541"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74796232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To explore the prognosis value of PT, DD and PLT and validation of the efficacy of the SIC score on initiating anticoagulant therapy in severe COVID-19 patients Methods: We retrospectively enrolled 420 consecutive patients with severe COVID-19 admitted to Tongji Hospital of Huazhong University of Science and Technology in Wuhan from January 1 to February 11, 2020 A retrospective review of the characteristics of these patients(mainly including PT,DD, PLT and SIC score at the point of meeting severity criteria, underlying disease and so on) were collected through the electronic medical record system of our hospital The medications for anticoagulant therapy and outcomes (28-day mortality event) were also monitored 1 The patients were divided into Survivor group and Non-survivor group based on the event of 28 d motality the parameters of coagulation tests and clinical characteristics between these two groups were compared Furthermore, the association between PT, DD, PLT and 28-day mortality were analyzed by logistic regression analysis The predictive value of PT, DD, PLT for 28-day mortality were explored by ROC curve analysis 2 Based on the SIC score, the patients were divided into SIC group and Non-SIC group The 28-day mortality rates were compared among cases which were receiving anticoagulant therapy or not in SIC group and Non-SIC group The logistic regression was performed to validate the receiving anticoagulant therapy and 28-day mortality between SIC group and Non-SIC group Results: 1 PT in Non-survivor group was significantly prolonged compared with Survivor group[15 1(14 2, 16 6)s vs 14 3(13 6, 15 1s, Z=-5 922, P14 5s, DD>3 0 μg/ml and PLT14 5 s, DD>3 0 μg/ml and PLT<125×109/L were independent risk factors for 28-day mortality of severe COVID-19 patients;Initiating anticoagulant therapy guided by SIC score may improve the outcome of the specific patients with severe COVID-19
{"title":"The prognosis value of PT, DD and PLT and validation of the efficacy of the SIC score on initiating anticoagulant therapy in severe COVID-19 patients","authors":"Huan Bai, Ling-Yan Shen, Xing Chen, Jiale Gong, Weiyong Liu, Ziyong Sun, N. Tang","doi":"10.3760/CMA.J.CN114452-20200323-00290","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200323-00290","url":null,"abstract":"Objective: To explore the prognosis value of PT, DD and PLT and validation of the efficacy of the SIC score on initiating anticoagulant therapy in severe COVID-19 patients Methods: We retrospectively enrolled 420 consecutive patients with severe COVID-19 admitted to Tongji Hospital of Huazhong University of Science and Technology in Wuhan from January 1 to February 11, 2020 A retrospective review of the characteristics of these patients(mainly including PT,DD, PLT and SIC score at the point of meeting severity criteria, underlying disease and so on) were collected through the electronic medical record system of our hospital The medications for anticoagulant therapy and outcomes (28-day mortality event) were also monitored 1 The patients were divided into Survivor group and Non-survivor group based on the event of 28 d motality the parameters of coagulation tests and clinical characteristics between these two groups were compared Furthermore, the association between PT, DD, PLT and 28-day mortality were analyzed by logistic regression analysis The predictive value of PT, DD, PLT for 28-day mortality were explored by ROC curve analysis 2 Based on the SIC score, the patients were divided into SIC group and Non-SIC group The 28-day mortality rates were compared among cases which were receiving anticoagulant therapy or not in SIC group and Non-SIC group The logistic regression was performed to validate the receiving anticoagulant therapy and 28-day mortality between SIC group and Non-SIC group Results: 1 PT in Non-survivor group was significantly prolonged compared with Survivor group[15 1(14 2, 16 6)s vs 14 3(13 6, 15 1s, Z=-5 922, P14 5s, DD>3 0 μg/ml and PLT14 5 s, DD>3 0 μg/ml and PLT<125×109/L were independent risk factors for 28-day mortality of severe COVID-19 patients;Initiating anticoagulant therapy guided by SIC score may improve the outcome of the specific patients with severe COVID-19","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"18 1","pages":"1205-1211"},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90005779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During the COVID-19 pandemic, the laboratory department plays an important role in diagnosis, treatment, curative effect evaluation and prognosis assessment of emerging infectious diseases It is of great importance that Huoshenshan Hospital, the designated hospital for COVID-19, was able to set up and carry out routine laboratory tests and Coronavirus nucleic acid test quickly and efficiently This paper aims to summarize the experience of the emergent inspection and support system in Huoshenshan Hospital and provide ideas and references for the construction of the emergent inspection and support system in other newly built infectious disease hospitals
{"title":"Construction of emergency laboratory medicine system of newly-built infectious disease hospitals in the context of COVID-19 pandemic","authors":"Pengnan Zhao, Xinyi Xia, Zhihua Wang, Changjun Wang, Hui Lu, B. Liu, Weiling Fu, Hongyan Zhang","doi":"10.3760/CMA.J.CN114452-20200408-00376","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200408-00376","url":null,"abstract":"During the COVID-19 pandemic, the laboratory department plays an important role in diagnosis, treatment, curative effect evaluation and prognosis assessment of emerging infectious diseases It is of great importance that Huoshenshan Hospital, the designated hospital for COVID-19, was able to set up and carry out routine laboratory tests and Coronavirus nucleic acid test quickly and efficiently This paper aims to summarize the experience of the emergent inspection and support system in Huoshenshan Hospital and provide ideas and references for the construction of the emergent inspection and support system in other newly built infectious disease hospitals","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"209 1","pages":"1118-1123"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77004834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the value of peripheral blood lymphocytes and lymphocyte subsets in the diagnosis of illness severity and prognosis of coronavirus disease 2019 (COVID-19) Methods: A retrospective analysis was performed on 179 COVID-19 patients admitted to the optical valley and Sino-French new branch district of Tongji Hospital in Wuhan, from February 8th, 2020 to February 18th, 2020 The included patients were divided into a mild group (n=84), a severe group (n=58) and a critical group (n=37) The median age of recruited patients was 65, including 107 males and 72 females ROC analysis was performed to determine the best threshold value of the lymphocyte subsets in distinguishing critical patients from severe or mild patients GraphPad Prism6 and SPSS19 0 statistical software were used for data analysis Differences among groups were analyzed using the Mann-Whitney U test Results: On admission, the white blood cell (WBC) of the critical patients were significantly higher than that of the mild and severe patients (P<0 001), and the lymphocytes of the mild patients were significantly higher than that of the critical and severe patients (P<0 001) The counts of T+B+NK, CD3, CD4, CD8, and NK cells were significantly decreased with the increase of illness severity (P<0 001) while the CD4/CD8 ratio in the critical group was significantly higher than that of the mild and severe groups (mild vs critical, P=0 002;severe vs critical, P=0 030) Furthermore, the receiver operating characteristic (ROC) analysis revealed that T+B+NK and CD3 showed the highest area under ROC for the identification of critical cases Then the mild and severe cases were combined into one group and compared with the critical group Neutrophil-to-lymphocyte ratio (NLR), neutrophil-to-CD3, neutrophil-to-CD4 and neutrophil-to-CD8 were used to assess illness severity, and our data showed that NLR was the best marker The lymphocytes of the mild and severe patients were increased ten days after admission with improvement of the disease However, the lymphocytes of the critical patients were progressively decreased with the deterioration of diseases Conclusions: The decrease of T+B+NK and CD3 cells, the increase of CD4/CD8 ratio and NLR serve as significant factors for identification of severe COVID-19 cases The dynamic monitoring of total lymphocytes and subsets has potential clinical value in predicting the prognosis of COVID-19
{"title":"Characteristics of peripheral blood lymphocytes and lymphocyte subsets in COVID-19 patients","authors":"Tingmei Wang, Hongyan Hou, Feng Wang, Min Huang, Minxia Zhang, Shiji Wu","doi":"10.3760/CMA.J.CN114452-20200407-00368","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200407-00368","url":null,"abstract":"Objective: To investigate the value of peripheral blood lymphocytes and lymphocyte subsets in the diagnosis of illness severity and prognosis of coronavirus disease 2019 (COVID-19) Methods: A retrospective analysis was performed on 179 COVID-19 patients admitted to the optical valley and Sino-French new branch district of Tongji Hospital in Wuhan, from February 8th, 2020 to February 18th, 2020 The included patients were divided into a mild group (n=84), a severe group (n=58) and a critical group (n=37) The median age of recruited patients was 65, including 107 males and 72 females ROC analysis was performed to determine the best threshold value of the lymphocyte subsets in distinguishing critical patients from severe or mild patients GraphPad Prism6 and SPSS19 0 statistical software were used for data analysis Differences among groups were analyzed using the Mann-Whitney U test Results: On admission, the white blood cell (WBC) of the critical patients were significantly higher than that of the mild and severe patients (P<0 001), and the lymphocytes of the mild patients were significantly higher than that of the critical and severe patients (P<0 001) The counts of T+B+NK, CD3, CD4, CD8, and NK cells were significantly decreased with the increase of illness severity (P<0 001) while the CD4/CD8 ratio in the critical group was significantly higher than that of the mild and severe groups (mild vs critical, P=0 002;severe vs critical, P=0 030) Furthermore, the receiver operating characteristic (ROC) analysis revealed that T+B+NK and CD3 showed the highest area under ROC for the identification of critical cases Then the mild and severe cases were combined into one group and compared with the critical group Neutrophil-to-lymphocyte ratio (NLR), neutrophil-to-CD3, neutrophil-to-CD4 and neutrophil-to-CD8 were used to assess illness severity, and our data showed that NLR was the best marker The lymphocytes of the mild and severe patients were increased ten days after admission with improvement of the disease However, the lymphocytes of the critical patients were progressively decreased with the deterioration of diseases Conclusions: The decrease of T+B+NK and CD3 cells, the increase of CD4/CD8 ratio and NLR serve as significant factors for identification of severe COVID-19 cases The dynamic monitoring of total lymphocytes and subsets has potential clinical value in predicting the prognosis of COVID-19","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"28 1","pages":"1093-1099"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79440469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cases of 2019-nCoV nucleic acid and antibody (IgM and IgG total antibody) after discharge from a hospital in Chongqing were continuously monitored It was found that 5 cases of "re-positive" phenomenon, 5 cases of antibody were positive, and there was a trend of increasing with time "Re-Positive" may be related to the following three factors Children with asymptomatic infection had a long time of fecal detoxification There were two consecutive nucleic acid tests "false negative" caused by various reasons The virus clearance in patients was not complete, and the discharge standard was not conservative enough The analysis of the causes of "Re-Positive" patients and the discussion of its infection will help us reveal more characteristics of this virus, and to provide a new basis for the discharge standard in the constantly updated diagnosis and treatment programme
{"title":"Re-positive nucleic acid detection in COVID-19 patients after discharge from hospital","authors":"Shenshen Zhi, Yi Xu, Yaobin Chen, Xiaoqin Meng, Wei Zhang, Qin-Hua Zhang, Weizhi Bai, Yingyi Zhou, Yu Luo, Li-jie Li, Jianda Liu, Wei Li","doi":"10.3760/CMA.J.CN114452-20200303-00170","DOIUrl":"https://doi.org/10.3760/CMA.J.CN114452-20200303-00170","url":null,"abstract":"Cases of 2019-nCoV nucleic acid and antibody (IgM and IgG total antibody) after discharge from a hospital in Chongqing were continuously monitored It was found that 5 cases of \"re-positive\" phenomenon, 5 cases of antibody were positive, and there was a trend of increasing with time \"Re-Positive\" may be related to the following three factors Children with asymptomatic infection had a long time of fecal detoxification There were two consecutive nucleic acid tests \"false negative\" caused by various reasons The virus clearance in patients was not complete, and the discharge standard was not conservative enough The analysis of the causes of \"Re-Positive\" patients and the discussion of its infection will help us reveal more characteristics of this virus, and to provide a new basis for the discharge standard in the constantly updated diagnosis and treatment programme","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"42 1","pages":"923-926"},"PeriodicalIF":0.0,"publicationDate":"2020-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74914388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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