Pub Date : 2003-12-01DOI: 10.1016/S1477-3627(03)02380-8
Darren R. Flower
{"title":"Benzon Symposium no. 50: the lipocalin protein superfamily","authors":"Darren R. Flower","doi":"10.1016/S1477-3627(03)02380-8","DOIUrl":"10.1016/S1477-3627(03)02380-8","url":null,"abstract":"","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 6","pages":"Pages 235-236"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02380-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77462708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-12-01DOI: 10.1016/S1477-3627(03)02383-3
Zoltán Konthur , Reto Crameri
One of the key issues of molecular biology at the beginning of the 21st century is the disclosure of complex interactions involved in cellular function. The availability of sequenced genomes sets the stage for functional analysis of the proteins. However, this task demands new technologies to warrant the characterisation of interactions at a higher pace. Here, we discuss phage display as one of the emerging technologies to address functional relationships between proteins and give examples of its use by focusing on antibody generation for protein expression analysis, discovery of novel disease markers and therapeutic targets, and mapping of protein–protein interactions.
{"title":"High-throughput applications of phage display in proteomic analyses","authors":"Zoltán Konthur , Reto Crameri","doi":"10.1016/S1477-3627(03)02383-3","DOIUrl":"10.1016/S1477-3627(03)02383-3","url":null,"abstract":"<div><p>One of the key issues of molecular biology at the beginning of the 21<sup>st</sup> century is the disclosure of complex interactions involved in cellular function. The availability of sequenced genomes sets the stage for functional analysis of the proteins. However, this task demands new technologies to warrant the characterisation of interactions at a higher pace. Here, we discuss phage display as one of the emerging technologies to address functional relationships between proteins and give examples of its use by focusing on antibody generation for protein expression analysis, discovery of novel disease markers and therapeutic targets, and mapping of protein–protein interactions.</p></div>","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 6","pages":"Pages 261-270"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02383-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80587972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-12-01DOI: 10.1016/S1477-3627(03)02379-1
Aleksandar Milosavljevic , Miklós Csürös , George Weinstock , Richard A. Gibbs
Comparative genomic sequencing and analysis offers new wealth of information for target selection and the development of therapeutics. This article focuses on the following two key innovations in mapping and sequencing: first, shotgun sequencing of clone pools to combine the benefits of whole-genome shotgun and clone-by-clone strategies, and second, the leveraging of newly available assembled genomic sequences to improve the effectiveness of new sequencing projects through comparative mapping and comparative sequence assembly. The following specific sequencing and mapping methods are discussed in detail: clone-array pooled shotgun sequencing (CAPSS); transversal shotgun pooling designs; clone-array pooled shotgun mapping (CAPS-MAP); pooled genomic indexing (PGI); short-tag pooled genomic indexing (ST-PGI); and comparative sequence assembly (the CSA™ method). The methods can be implemented with only modest modifications of current large-scale sequencing pipelines and are highly synergistic with the next generation of sequencing technologies.
{"title":"Shotgun sequencing, clone pooling, and comparative strategies for mapping and sequencing","authors":"Aleksandar Milosavljevic , Miklós Csürös , George Weinstock , Richard A. Gibbs","doi":"10.1016/S1477-3627(03)02379-1","DOIUrl":"10.1016/S1477-3627(03)02379-1","url":null,"abstract":"<div><p>Comparative genomic sequencing and analysis offers new wealth of information for target selection and the development of therapeutics. This article focuses on the following two key innovations in mapping and sequencing: first, shotgun sequencing of clone pools to combine the benefits of whole-genome shotgun and clone-by-clone strategies, and second, the leveraging of newly available assembled genomic sequences to improve the effectiveness of new sequencing projects through comparative mapping and comparative sequence assembly. The following specific sequencing and mapping methods are discussed in detail: clone-array pooled shotgun sequencing (CAPSS); transversal shotgun pooling designs; clone-array pooled shotgun mapping (CAPS-MAP); pooled genomic indexing (PGI); short-tag pooled genomic indexing (ST-PGI); and comparative sequence assembly (the CSA™ method). The methods can be implemented with only modest modifications of current large-scale sequencing pipelines and are highly synergistic with the next generation of sequencing technologies.</p></div>","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 6","pages":"Pages 245-252"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02379-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87583986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-12-01DOI: 10.1016/S1477-3627(03)02385-7
Roberta Friedman, BMN News
{"title":"Finding the right tools for proteomics","authors":"Roberta Friedman, BMN News","doi":"10.1016/S1477-3627(03)02385-7","DOIUrl":"https://doi.org/10.1016/S1477-3627(03)02385-7","url":null,"abstract":"","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 6","pages":"Page 229"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02385-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92061663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-12-01DOI: 10.1016/S1477-3627(03)02381-X
David B. Rozema, David L. Lewis
Inhibition of gene expression using the RNA interference (RNAi) pathway is rapidly becoming the method of choice for studying gene function in mammalian cells. However, successful knockdown of the target gene requires efficient delivery of short interfering RNAs (siRNAs). Several technologies have been developed that enable effective delivery of siRNAs to both cells in culture and whole animals. These technologies will allow the use of RNAi to study gene function in mammalian model systems in which classical methods are often limited and costly.
{"title":"siRNA delivery technologies for mammalian systems","authors":"David B. Rozema, David L. Lewis","doi":"10.1016/S1477-3627(03)02381-X","DOIUrl":"10.1016/S1477-3627(03)02381-X","url":null,"abstract":"<div><p>Inhibition of gene expression using the RNA interference (RNAi) pathway is rapidly becoming the method of choice for studying gene function in mammalian cells. However, successful knockdown of the target gene requires efficient delivery of short interfering RNAs (siRNAs). Several technologies have been developed that enable effective delivery of siRNAs to both cells in culture and whole animals. These technologies will allow the use of RNAi to study gene function in mammalian model systems in which classical methods are often limited and costly.</p></div>","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 6","pages":"Pages 253-260"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02381-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83617406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-10-01DOI: 10.1016/S1477-3627(03)02360-2
K.K. Jain
{"title":"Proteomics for drug target and biomarker identification","authors":"K.K. Jain","doi":"10.1016/S1477-3627(03)02360-2","DOIUrl":"10.1016/S1477-3627(03)02360-2","url":null,"abstract":"","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 5","pages":"Pages 189-190"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02360-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89005321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-10-01DOI: 10.1016/S1477-3627(03)02370-5
Philip E. Bourne
{"title":"The status of structural genomics","authors":"Philip E. Bourne","doi":"10.1016/S1477-3627(03)02370-5","DOIUrl":"10.1016/S1477-3627(03)02370-5","url":null,"abstract":"","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 5","pages":"Pages 181-182"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02370-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88644992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-10-01DOI: 10.1016/S1477-3627(03)02376-6
Helen Dell
{"title":"Positive outlook for reverse vaccinology","authors":"Helen Dell","doi":"10.1016/S1477-3627(03)02376-6","DOIUrl":"10.1016/S1477-3627(03)02376-6","url":null,"abstract":"","PeriodicalId":101208,"journal":{"name":"TARGETS","volume":"2 5","pages":"Page 184"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1477-3627(03)02376-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80387259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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