The Journal of Trace Elements in Experimental Medicine最新文献_第7页

Attenuation by boron supplementation of the biochemical changes associated with thioacetamide-induced hepatic lesions 补硼对硫代乙酰胺肝损伤相关生化变化的影响

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10000

Shakir Ali, G. Diwakar, Sonica Pawa, M.R. Siddiqui, S.K. Jain, M Abdulla

Acute hepatic failure is a severe complication induced by certain chemicals, drugs, or virus. Thioacetamide generally has been used for the study of hepatic failure in experimental animal model. The present study was aimed at to examine the role of boron in the pathogenesis of acute hepatic failure in rats. A single intraperitoneal injection of thioacetamide produced severe liver injury, as manifested by elevation in serum aminotransferases, alkaline phosphatase, and hepatic lipid peroxidation. Boron, when administered in the form of boric acid for three consecutive days followed by thioace-tamide, attenuated thioacetamide-mediated changes in the level of these biochemical parameters in a dose-dependent manner. The effect of boron supplementation on the survival rates of rats treated with a lethal dose of thioacetamide was also determined and found to lower the mortality rates in the group of animals supplemented with boron followed by thioacetmide. The effects on biochemical parameters and the survival rates were dependent on the dose of boron administered as boric acid. It is concluded that boron provides protection against the thioacetamide-induced acute hepatic failure in rats in a dose-dependent manner. J. Trace Elem. Exp. Med. 15: 47–55, 2002. © 2002 Wiley-Liss, Inc.

急性肝功能衰竭是由某些化学物质、药物或病毒引起的严重并发症。硫代乙酰胺通常用于实验动物模型中肝衰竭的研究。本研究旨在探讨硼在大鼠急性肝功能衰竭发病机制中的作用。单次腹膜内注射硫代乙酰胺会导致严重的肝损伤，表现为血清转氨酶、碱性磷酸酶和肝脏脂质过氧化升高。当硼以硼酸的形式连续给药三天，然后给药硫代乙酰胺时，以剂量依赖的方式减弱了硫代乙酰胺介导的这些生化参数水平的变化。还测定了补充硼对用致死剂量的硫代乙酰胺治疗的大鼠存活率的影响，并发现补充硼后补充硫代乙酰胺的动物组的死亡率较低。对生化参数和存活率的影响取决于以硼酸形式给予的硼的剂量。结论硼对硫代乙酰胺诱导的大鼠急性肝功能衰竭具有剂量依赖性保护作用。J.Trace Elem。Exp.Med.15:47-551002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 4

Optimal dietary concentrations of vitamin C and chromium for alleviating the effect of low ambient temperature on serum insulin, corticosterone, and some blood metabolites in laying hens 维生素C和铬的最佳日粮浓度减轻低温对蛋鸡血清胰岛素、皮质酮和某些血液代谢产物的影响

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10014

K. Şahin, M. Önderci

This experiment was conducted to evaluate the effects of vitamin C (L-ascorbic acid) and chromium (chromium picolinate, CrPic) on serum insulin, corticosterone, and some metabolites in laying hens (Hy-Line) reared under a low ambient temperature (6.2°C). One hundred eighty laying hens (32 weeks old) were divided into six groups, with 30 hens per group. The experiment was designed in a 2 × 3 factorial arrangement using two levels of vitamin C (125 and 250 mg/kg of diet) and three levels of CrPic (200, 400, or 800 μg/kg of diet). The highest values for insulin, total protein, and albumin (P = 0.001) and the lowest values for corticosterone, glucose, triglyceride, and cholesterol (P = 0.001) were obtained when 250 mg of vitamin C was included with either 400 or 800 μg Cr per kg of diet. Interaction between vitamin C and chromium was detected for only insulin (P = 0.03). Serum activities of SGOT and SGPT were not influenced by dietary vitamin C or chromium (P > 0.45); however, serum activity of AP increased (P = 0.001). Increasing both dietary vitamin C and chromium caused an increase in serum concentrations of Ca, P, and K (P = 0.001) but a decrease in serum concentrations of Na (P = 0.001). The results of the present study suggest that dietary supplements of vitamin C and chromium alter serum hormone and metabolite status of laying hens and that supplementing a combination of vitamin C (250 mg/kg of diet) and chromium (400 or 800 μg Cr/kg of diet) can positively influence the performance of laying hens reared under low ambient temperature. J. Trace Elem. Exp. Med. 15:153–161, 2002. © 2002 Wiley-Liss, Inc.

本试验旨在评价维生素C（L-抗坏血酸）和铬（吡啶甲酸铬，CrPic）对低环境温度（6.2°C）饲养的蛋鸡（Hy-Line）血清胰岛素、皮质酮和某些代谢产物的影响。该实验采用2×3析因安排，使用两个水平的维生素C（125和250 mg/kg饮食）和三个水平的CrPic（200、400或800μg/kg饮食）。当每公斤饮食中含有250 mg维生素C和400或800μg Cr时，胰岛素、总蛋白和白蛋白的最高值（P=0.001）和皮质酮、葡萄糖、甘油三酯和胆固醇的最低值（P=0.000）。维生素C和铬之间的相互作用仅在胰岛素中检测到（P=0.03）。血清SGOT和SGPT活性不受维生素C或铬的影响（P>；0.45）；血清AP活性升高（P=0.001），本研究结果表明，维生素C和铬的膳食补充剂可以改变蛋鸡的血清激素和代谢产物状态，维生素C（250 mg/kg）和铬（400或800μg Cr/kg）的联合补充可以对蛋鸡的生产性能产生积极影响在低环境温度下饲养。J.Trace Elem。《实验医学》，15:153-1612002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 12

Zinc in liver disease 肝病中的锌

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10002

K. Grüngreiff

The essentiality of zinc for humans was first documented by Prasad in the 1960s. During the past 35 years, zinc deficiency in humans a result of nutritional factors and several disease states has been recognized. Many of the clinical features of liver cirrhosis have been linked to zinc deficiency, including loss of body hair, testicular atrophy, poor appetite, immune dysfunction, altered taste and smell, reduced vitamin A and thyroid hormone metabolism, altered protein metabolism, delayed wound healing, and diminished drug elimination capacity. One of the most interesting and novel aspects concerning the presumable role of zinc deficiency in producing clinical features of liver cirrhosis is the possible relationship between zinc and hepatic encephalopathy (HE). Long-term zinc supplementation in patients with HE improves neurological symptoms and metabolic parameters. In Wilson's disease, an inherited defect of hepatic copper, zinc is used for maintenance as well as treating presymptomatic, pregnant, and pediatric patients. Zinc may be involved in the pathogenesis of chronic hepatitis C. This work is an attempt to review the information available in this field to understand the important role that zinc plays in the pathogenesis and therapy of several liver diseases. J. Trace Elem. Exp. Med. 15:67–78, 2002. © 2002 Wiley-Liss, Inc.

普拉萨德在20世纪60年代首次记录了锌对人类的重要性。在过去的35年里，人们已经认识到，人类缺锌是营养因素和几种疾病状态的结果。肝硬化的许多临床特征与缺锌有关，包括体毛脱落、睾丸萎缩、食欲不佳、免疫功能障碍、味觉和嗅觉改变、维生素A和甲状腺激素代谢降低、蛋白质代谢改变、伤口愈合延迟和药物消除能力下降。关于锌缺乏在肝硬化临床特征产生中的可能作用，最有趣和最新颖的方面之一是锌与肝性脑病（HE）之间的可能关系。HE患者长期补充锌可改善神经系统症状和代谢参数。Wilson病是一种遗传性肝脏铜缺陷，锌用于维持和治疗症状前、孕妇和儿童患者。锌可能参与慢性丙型肝炎的发病机制。这项工作试图综述该领域的现有信息，以了解锌在几种肝病的发病机制和治疗中的重要作用。J.Trace Elem。Exp.Med.15:67-782002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 20

Effects of dietary chromium picolinate supplementation on serum and tissue mineral contents of laying Japanese quails 日粮添加吡啶甲酸铬对蛋鸡血清和组织矿物质含量的影响

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10013

Kazim Şahin, Nurhan Şahin, Osman Küçük

This experiment was conducted to evaluate the effects of chromium (chromium picolinate, CrPic) on serum and tissue concentrations of Cr, Zn, Cu, Fe, and Mg of laying Japanese quails (Coturnix coturnix japonica). One hundred fifty laying Japanese quails (45 days old) were divided into five groups, 30 quails per group. The laying quails were fed either the control diet containing 1,285.96 μg of Cr/kg of diet or the control diet supplemented with 200, 400, 800, or 1,200 μg of Cr/kg of diet. Increasing dietary chromium supplementation linearly increased serum chromium and zinc concentrations (P = 0.01) but linearly decreased (P = 0.01) copper serum concentrations. Serum iron and magnesium concentrations did not change (P > 0.10) as dietary chromium supplementation increased. Similar response to Cr supplementation was also observed in tissue mineral concentrations. Liver, longissimus muscle, and kidney chromium and zinc concentrations linearly increased (P = 0.01), whereas copper concentrations linearly decreased (P = 0.01) as dietary chromium supplementation increased. Also, liver, longissimus muscle, and kidney iron and magnesium concentrations did not change (P > 0.10) as dietary chromium supplementation increased. The results of present study conclude that supplemental chromium to the diet of Japanese quails influences serum and tissue Cr, Zn, and Cu concentrations, thus most probably functions of the organs as well as the welfare of Japanese quails. J. Trace Elem. Exp. Med. 15:163–169, 2002. © 2002 Wiley-Liss, Inc.

本试验旨在评价铬（吡啶甲酸铬，CrPic）对日本鹌鹑（Coturnix Coturnix japonica）血清和组织中Cr、Zn、Cu、Fe和Mg浓度的影响。将150只日本产鹌鹑（45日龄）分为5组，每组30只。日粮中铬含量为1285.96μg/kg，日粮中Cr含量为200、400、800或1200μg/kg。增加日粮铬补充量可线性增加血清铬和锌浓度（P=0.01），但可线性降低铜血清浓度（P<0.01）。血清铁和镁浓度没有随着膳食铬补充量的增加而改变（P＞0.10）。在组织矿物质浓度中也观察到对Cr补充的类似反应。肝脏、最长肌和肾脏的铬和锌浓度随着膳食铬补充量的增加而线性增加（P=0.01），而铜浓度则线性降低（P=0.001）。此外，肝脏、最长肌和肾脏的铁和镁浓度没有随着膳食铬补充量的增加而改变（P>；0.10）。本研究的结果表明，日本鹌鹑日粮中添加铬会影响血清和组织中Cr、Zn和Cu的浓度，因此很可能影响日本鹌鹑的器官功能和福利。J.Trace Elem。Exp.Med.15:163-1692002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 13

Hypothalamic digoxin and hypomagnesemia in relation to the pathogenesis of multiple sclerosis 下丘脑地高辛和低镁血症与多发性硬化症发病机制的关系

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10017

Ravi Kumar Kurup, Parameswara Achutha Kurup

This study assessed the isoprenoid pathway-related biochemical cascade in multiple sclerosis, and in this work we discuss the pivotal role of hypothalamic digoxin and membrane sodium-potassium ATPase inhibition in the pathogenesis of multiple sclerosis. Our results showed that there was an elevation in plasma HMG CoA reductase activity, serum digoxin, and dolichol levels and a reduction in red blood cell (RBC) membrane sodium potassium ATPase activity, serum ubiquinone, and magnesium levels. Serum tryptophan, serotonin, strychnine, nicotine, and quinolinic acid were elevated whereas tyrosine, dopamine, morphine, and noradrenaline were decreased. The total serum glycosaminoglycans, hyaluronic acid, heparin, and heparan sulphate were elevated and levels of chondroitin sulphates and dermatan sulphate were reduced in multiple sclerosis. The serum glycolipids as well as the hexose, fucose, and sialic acid carbohydrate residues of serum glycoproteins were elevated. The activity of glycosaminoglycan degrading enzymes was elevated. The activity of glycohydrolases—beta galactosidase and beta fucosidase—were increased and beta glucosidase reduced in the serum. The RBC membrane glycosaminoglycan, the hexose and fucose carbohydrate residues of glycoproteins, and the cholesterol:phospholipid ratio of the RBC membrane were decreased in multiple sclerosis. Membrane Na+-K+ ATPase inhibition-related increased intracellular calcium can produce immune activation via the calcineurin signal transduction system in T cells. Digoxin-induced altered glycoconjugate metabolism can change the structural integrity of myelin as well as result in immune dysregulation caused by defective presentation of myelin glycoprotein antigens to CD8 cells. Digoxin-induced defective membrane formation of oligodendrocyte membrane can inhibit remyelination. Digoxin-related mitochondrial dysfunction can contribute to free radical generation, microglial activation, and oligodendrocyte apoptosis that are important in demyelination. J. Trace Elem. Exp. Med. 15:211–220, 2002. © 2002 Wiley-Liss, Inc.

本研究评估了多发性硬化症中类异戊二烯途径相关的生化级联反应，并在本工作中讨论了下丘脑地高辛和膜钠钾ATP酶抑制在多发性痴呆症发病机制中的关键作用。我们的研究结果表明，血浆HMG-CoA还原酶活性、血清地高辛和dolichol水平升高，红细胞（RBC）膜钠钾ATP酶活性、血清泛醌和镁水平降低。血清色氨酸、血清素、士的宁、尼古丁和喹啉酸升高，而酪氨酸、多巴胺、吗啡和去甲肾上腺素降低。多发性硬化症患者血清总糖胺聚糖、透明质酸、肝素和硫酸乙酰肝素水平升高，硫酸软骨素和硫酸皮肤素水平降低。血清糖脂以及血清糖蛋白的己糖、岩藻糖和唾液酸碳水化合物残基升高。糖胺聚糖降解酶活性升高。血清中糖水解酶——β-半乳糖苷酶和β-岩藻糖苷酶的活性增加，β-葡萄糖苷酶降低。多发性硬化患者红细胞膜糖胺聚糖、糖蛋白的己糖和岩藻糖碳水化合物残基以及红细胞膜的胆固醇与磷脂比率降低。膜Na+-K+ATP酶抑制相关的细胞内钙增加可通过T细胞中的钙调神经磷酸酶信号转导系统产生免疫激活。地高辛诱导的糖缀合物代谢改变可以改变髓鞘的结构完整性，并导致由髓鞘糖蛋白抗原向CD8细胞的缺陷呈递引起的免疫失调。地高辛诱导的少突胶质细胞膜形成缺陷可抑制髓鞘再形成。地高辛相关的线粒体功能障碍可导致自由基生成、小胶质细胞活化和少突胶质细胞凋亡，这在脱髓鞘中很重要。J.Trace Elem。Exp.Med.15:211–220002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 1

Trace element levels in the experimental peritonitis 实验性腹膜炎的微量元素水平

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10006

Dildar Konukoğlu, Meltem Ercan, Erkal Ziylan

Electron transfer from iron or copper ions to oxygen is an important example of cellular free radical initiation. Oxygen-derived free radicals have been implicated as mediators of cellular injury in several model systems. To evaluate the importance of iron, copper, and zinc levels on lipid peroxidation in peritonitis, we measured peritoneum malondialdehyde (MDA) as a marker of lipid peroxidation, zinc, copper, and iron levels during an animal model of intraperitoneal sepsis. Additionally, the effects of free radical scavenger alpha-tocopherol administration were studied. The peritoneum MDA, iron, copper, and zinc levels were increased after induction of peritonitis with Escherichia coli. The treatment with alpha-tocopherol decreased peritoneum MDA, iron, and copper levels significantly but not the zinc levels. Additionally, the treatment with alpha-tocopherol 3 days prior to injection of E. coli decreased MDA, copper, and iron levels more than treatment with alpha-tocopherol at the time of injection of E. coli. Our results indicate that copper, iron, and zinc have important effects on peroxidation events in E. coli-induced peritonitis and that alpha-tocopherol treatment can improve the oxidant status. J. Trace Elem. Exp. Med. 15:79–84, 2002. © 2002 Wiley-Liss, Inc.

从铁或铜离子到氧的电子转移是细胞自由基引发的一个重要例子。在几个模型系统中，氧衍生的自由基被认为是细胞损伤的介质。为了评估铁、铜和锌水平对腹膜炎脂质过氧化的重要性，我们在腹膜内败血症动物模型中测量了作为脂质过氧化标志的腹膜丙二醛（MDA）、锌、铜和铁水平。此外，还研究了自由基清除剂α-生育酚给药的效果。用大肠杆菌诱导腹膜炎后，腹膜MDA、铁、铜和锌水平升高。α-生育酚治疗显著降低腹膜MDA、铁和铜水平，但不降低锌水平。此外，与注射大肠杆菌时用α-生育酚处理相比，在注射大肠杆菌前3天用α-生育醇处理更能降低MDA、铜和铁水平。我们的研究结果表明，铜、铁和锌对大肠杆菌诱导的腹膜炎的过氧化事件有重要影响，α-生育酚处理可以改善氧化状态。J.Trace Elem。Exp.Med.15:79-842002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 0

Hypothalamic digoxin and hypomagnesemia in human pre-eclampsic toxemia, cortical venous thrombosis, and postpartum psychosis 人类先兆子痫毒血症、皮质静脉血栓形成和产后精神病患者的下丘脑地高辛和低镁血症

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10015

Ravi Kumar Kurup, Parameswara Achutha Kurup

The isoprenoid pathway produces three key metabolites—digoxin (membrane sodium-potassium ATPase inhibitor and regulator of neurotransmitter transport), dolichol (regulates N-glycosylation of proteins), and ubiquinone (free radical scavenger). The pathway was assessed in patients with pre-eclampsic toxaemia, cortical venous thrombosis, and postpartum psychosis. It was also studied for comparison in patients with right hemispheric, left hemispheric, and bihemispheric dominance. The results of the study showed that the isoprenoid pathway was upregulated with increased digoxin synthesis in all the three groups of patients. There was also a reduction in red blood cell (RBC) membrane Na+–K+ ATPase activity and serum magnesium levels. There was an increase in serum tryptophan catabolites and reduction in tyrosine catabolites. The serum dolichol and glycoconjugate levels were increased and lysosomal stability reduced with increased plasma lysosomal enzymes in all the three groups. The serum ubiquinone levels were low, and RBC free radical parameters increased. The RBC membrane cholesterol: phospholipid ratio was increased, and glycoconjugate was reduced in the membrane of these patients. This pattern correlated with those in right hemispheric dominance. The isoprenoid pathway and hypothalamic digoxin may thus play a crucial role in the pathogenesis of CVT, PET, and postpartum psychosis. J. Trace Elem. Exp. Med. 15:171–190 2002. © 2002 Wiley-Liss, Inc.

类异戊二烯途径产生三种关键代谢产物——地高辛（膜钠钾ATP酶抑制剂和神经递质转运调节剂）、多力酚（调节蛋白质的N-糖基化）和泛醌（自由基清除剂）。该途径在先兆子痫毒血症、皮质静脉血栓形成和产后精神病患者中进行了评估。它还被研究用于右半球、左半球和双半球优势患者的比较。研究结果表明，在所有三组患者中，类异戊二烯途径都随着地高辛合成的增加而上调。红细胞（RBC）膜Na+–K+ATP酶活性和血清镁水平也有所降低。血清色氨酸分解代谢产物增加，酪氨酸分解代谢产物减少。在所有三组中，随着血浆溶酶体酶的增加，血清dolichol和糖缀合物水平增加，溶酶体稳定性降低。血清泛醌水平较低，红细胞自由基参数升高。这些患者的红细胞膜胆固醇与磷脂的比例增加，膜中的糖结合物减少。这种模式与右半球占优势的模式相关。因此，类异戊二烯途径和下丘脑地高辛可能在CVT、PET和产后精神病的发病机制中发挥关键作用。J.Trace Elem。《实验医学》，2002年15:171–190。©2002 Wiley-Liss，股份有限公司。

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引用次数: 2

Low levels of selenium in miscarriage 流产时硒含量低

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10004

Müzehher Güvenç, Hüseyin Güven, Fikret Karataş, A. Denizmen Aygün, Sırrı Bektaş

Few data are presented in the literature about selenium and miscarriage. The aim of this article was to study the relationship between the selenium status of pregnant women and miscarriage. A randomized, controlled trial was performed in Elazig State Hospital and Firat University Hospital. Serum and hair samples were obtained from 20 non-pregnant women, 32 healthy mothers with normal newborns, and 16 women who miscarried. Serum selenium levels, as ng/mL, and hair selenium levels, as ng/g, were determined on a Perkin–Elmer 1000 spectrophotometer by fluorometry. The mean serum and hair selenium concentrations of the women who miscarried (42.8 ± 2.7 ng/mL, 276 ± 6.6 ng/g, respectively) were significantly lower than those of the control healthy mothers (50.2 ± 2.3 ng/mL, 300 ± 6.1 ng/g, respectively) and the non-pregnant women (58.1 ± 3.1 ng/mL, 315 ± 7.6 ng/g, respectively). Maternal selenium deficiency during early gestation was thought to be one of the factors responsible in the pathogenesis of miscarriage. More studies on maternal selenium status during pregnancy are needed. J. Trace Elem. Med. 15:97–101, 2002. © 2002 Wiley-Liss, Inc.

文献中很少有关于硒和流产的数据。本文旨在研究孕妇硒含量与流产的关系。在埃拉泽州立医院和菲拉特大学医院进行了一项随机对照试验。从20名非孕妇、32名新生儿正常的健康母亲和16名流产妇女身上采集了血清和头发样本。在Perkin–Elmer 1000分光光度计上通过荧光测定法测定血清硒水平（ng/mL）和头发硒水平（ng/g）。流产妇女的平均血清和头发硒浓度（分别为42.8±2.7 ng/mL、276±6.6 ng/mL）显著低于对照健康母亲（分别为50.2±2.3 ng/mL、300±6.1 ng/mL）和非孕妇（分别为58.1±3.1 ng/mL、315±7.6 ng/mL）。妊娠早期母体硒缺乏被认为是导致流产的因素之一。需要对孕妇在怀孕期间的硒状况进行更多的研究。J.Trace Elem。Med.15:97–1011002。©2002 Wiley-Liss，股份有限公司。

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引用次数: 11

Trace elements content in serum, normal skin, and scar tissues of keloid and normal scar patients 瘢痕疙瘩和正常瘢痕患者血清、正常皮肤和瘢痕组织中微量元素含量

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10001

Rameshwar L. Bang, Abdul Latif Al-Bader, Prem N Sharma, Alice Babu Mattapallil, Abdulla I. Behbehani, Hussein Dashti

Wound healing is a normal physiological process that occurs after injury. Scars are usually formed, and the nature of the scar depends on the balance between the formed and degraded collagen during healing. A balanced production and degradation of collagen in wound healing results in the formation of a normal scar whereas an imbalance produces what is known as keloid scar. The factors that cause an imbalance are not well identified, although many factors, such as immunity, genetic, ischaemia, hormonal, growth, and nutritional factors, are blamed. This study was undertaken to investigate trace elements level in the serum, normal skin, and scar tissue of patients with normal and keloid scars. Copper (Cu), zinc (Zn), selenium (Se), and manganese (Mn) levels were estimated in 100 (50 with normal scars and the others with keloids) patients. The serum level of Zn was found to be higher in keloid scar patients, and this increase was highly significant (P < 0.0001). The level of Cu was significantly higher (P < 0.0001) in the normal skin and scar tissues of keloid patients. Trace element levels in scar tissues were observed to be lower in comparison to normal skin trace element content in both groups. The significant changes of Zn in serum and Cu in skin and scar tissues of keloid patients as noted in this study may suggest their role in the regulation of behavior of scar formation in wound healing. J. Trace Elem. Exp. Med. 15:57–66, 2002. © 2002 Wiley-Liss, Inc.

伤口愈合是受伤后发生的一个正常的生理过程。疤痕通常是形成的，疤痕的性质取决于愈合过程中形成和降解的胶原蛋白之间的平衡。在伤口愈合过程中，胶原蛋白的平衡产生和降解会形成正常的疤痕，而不平衡则会产生所谓的瘢痕疙瘩。导致失衡的因素还没有很好地确定，尽管许多因素，如免疫力、遗传、缺血、激素、生长和营养因素，都被归咎于此。本研究旨在调查正常瘢痕和瘢痕疙瘩患者血清、正常皮肤和瘢痕组织中的微量元素水平。估计了100名（50名正常瘢痕患者和其他瘢痕疙瘩患者）患者的铜（Cu）、锌（Zn）、硒（Se）和锰（Mn）水平。瘢痕疙瘩患者的血清Zn水平较高，并且这种增加非常显著（P<；0.0001）。瘢痕疙瘩患者正常皮肤和瘢痕组织中Cu水平显著较高（P<：0.0001）。与两组正常皮肤微量元素含量相比，瘢痕组织中的微量元素水平较低。如本研究所述，瘢痕疙瘩患者血清中Zn和皮肤及瘢痕组织中Cu的显著变化可能表明它们在伤口愈合中对瘢痕形成行为的调节作用。J.Trace Elem。《实验医学》，2002年15月57日至66日。©2002 Wiley-Liss，股份有限公司。

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引用次数: 18

Aluminum inhibition of microglial function in vitro 铝对小胶质细胞功能的抑制作用

The Journal of Trace Elements in Experimental Medicine

Pub Date : 2002-01-01 DOI: 10.1002/jtra.10012

Bei Ping He, Michael J. Strong

We have previously observed in vivo that chronic AlCl3 exposure in young adult New Zealand white rabbits induces a reversible motor neuron degeneration. In addition to the development of neurofilamentous aggregates within degenerating neurons, we have also observed a reduction of microglial number and activation. To determine if aluminum could directly effect microglial function in vivo, we used an immortalized murine microglial cell line (BV-2) to study the effect of organic (aluminum lactate) and inorganic (AlCl3) compounds on microglial function. We have observed a dose-dependent inhibition of phagocytosis, proliferation, migration, and release of TNF-α and nitric oxide in the absence of inducing microglial death. Both organic and inorganic aluminum decreased the extent of LPS-activated microglia-mediated death of a motor neuron hybridoma cell line (NSC 34). These findings demonstrate that aluminum compounds can directly affect microglial in vitro. J. Trace Elem. Exp. Med. 15:141–152, 2002. © 2002 Wiley-Liss, Inc.

我们之前在体内观察到，新西兰成年幼兔长期暴露于AlCl3会诱导可逆的运动神经元变性。除了退化神经元内神经丝状聚集体的发育外，我们还观察到小胶质细胞数量和活化的减少。为了确定铝是否可以在体内直接影响小胶质细胞的功能，我们使用永生化小鼠小胶质细胞系（BV-2）来研究有机（乳酸铝）和无机（AlCl3）化合物对小胶质细胞功能的影响。我们观察到，在不诱导小胶质细胞死亡的情况下，吞噬作用、增殖、迁移以及TNF-α和一氧化氮的释放受到剂量依赖性抑制。有机铝和无机铝都降低了LPS激活的小胶质细胞介导的运动神经元杂交瘤细胞系（NSC 34）死亡的程度。这些发现表明，铝化合物可以在体外直接影响小胶质细胞。J.Trace Elem。《实验医学》，2002年，15:141–152。©2002 Wiley-Liss，股份有限公司。

引用次数: 2