Clinical biochemistry最新文献_第2页

The expression of canopy FGF signaling regulator 2 serves as a diagnostic and prognostic indicator for NSCLC

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-02-10 DOI: 10.1016/j.clinbiochem.2025.110895

Xiao Jiang , Jun Chen , Shujun Ding , Jun Yin , Jiying Gu , Xiangming Fang

Background

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. The identification of new biomarkers is crucial for enhancing early detection and treatment outcomes. This study explores the role of Canopy FGF Signaling Regulator 2 (CNPY2) in NSCLC progression and its potential as a diagnostic and prognostic biomarker.

Methods

CNPY2 expression was analyzed in 228 NSCLC tumor samples and adjacent normal tissues using quantitative RT-PCR and ELISA. Serum CNPY2 levels were also measured in 160 healthy controls and NSCLC patients. The relationship between CNPY2 expression and clinicopathological features, including epithelial-mesenchymal transition (EMT) markers, was assessed. Receiver operator curve analysis was used to evaluate the diagnostic potential of serum CNPY2, while Kaplan-Meier survival analysis assessed its prognostic significance.

Results

CNPY2 levels were significantly elevated in NSCLC tissues compared to adjacent normal tissues. Higher CNPY2 expression was associated with larger tumor size, advanced T stage, and higher N stage. Furthermore, CNPY2 expression was positively correlated with Vimentin and N-cadherin, and negatively correlated with E-cadherin. Elevated serum CNPY2 levels in NSCLC patients demonstrated moderate diagnostic accuracy, with an area under the curve of 0.78. High CNPY2 expression was also linked to reduced overall survival (p = 0.001).

Conclusions

CNPY2 is markedly overexpressed in NSCLC and is associated with increased tumor aggressiveness and EMT. Serum CNPY2 shows promise as a non-invasive biomarker for NSCLC diagnosis, and elevated expression is correlated with a poorer prognosis. Thus, CNPY2 may serve as both a valuable biomarker and a potential therapeutic target in NSCLC.

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引用次数: 0

Cardiac function impairment in recipient twins of twin-to-twin transfusion syndrome: Insights from NT-proBNP levels in amniotic fluid

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-02-05 DOI: 10.1016/j.clinbiochem.2025.110894

Wenyan Jian , Dewei Guo , Ruojin Yao , Mi Pei , Manhui Guo , Fang Yang

Objectives

To investigate changes in N-terminal pro-brain natriuretic peptide (NT-proBNP) levels in the amniotic fluid of recipient twins with twin-twin transfusion syndrome (TTTS), analyze the correlation between NT-proBNP and cardiac linear measurements, and assess the feasibility of NT-proBNP as a biochemical marker for fetal cardiac function.

Design and methods

A total of 47 pregnancies with TTTS, 21 idiopathic polyhydramnios pregnancies, and 114 normal singleton pregnancies were included from Xiangya Hospital of Central South University between October 2020 and July 2023. Fetal cardiac linear parameters, amniotic fluid depth, and NT-proBNP levels in amniotic fluid were measured across the three groups. The correlation of NT-proBNP with amniotic fluid depth, cardiac linear parameters, and CHOP score in TTTS recipients was analyzed.

Results

There was no statistically significant difference in amniotic fluid NT-proBNP levels and cardiac linear parameters between idiopathic polyhydramnios and normal singletons. However, NT-proBNP levels and cardiac parameters in TTTS recipient twins were significantly higher than in the other two groups (p < 0.05). After adjusting for gestational variables, NT-proBNP levels in TTTS recipients showed significant correlations with atrial and ventricular diameters, ventricular wall thickness, cardiothoracic ratio, and CHOP score.

Conclusions

Amniotic fluid NT-proBNP is a sensitive and objective biochemical marker for assessing fetal cardiac function, independent of amniotic fluid volume. It serves as a valuable complement to echocardiographic assessment in evaluating the severity of fetal heart failure in TTTS recipients.

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引用次数: 0

Metabolism of Natriuretic peptides and impact on insulin resistance and fat mass in healthy subjects

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-02-04 DOI: 10.1016/j.clinbiochem.2025.110893

Timothy C.R. Prickett , Lynley K. Lewis , John F. Pearson , Eric A. Espiner

Background

Natriuretic peptides (NP) have important roles in regulating fat balance and metabolic health. Reduced concentrations of ANP and BNP in plasma are associated with increased insulin resistance in obesity. Whether this is due to increased clearance or reduced bioactivity of immunoreactive NP forms is unclear.

Design and Method

These questions were addressed in a community study of mildly obese subjects at middle age. The ratio of amino-terminal (NT) pro-NP to bioactive C-terminal NP was used as a putative index of the clearance of bioactive forms.

Results

Lower ratios of amino-terminal pro-NP to bioactive C-terminal NP were associated with increased insulin resistance. In linear regression models, NT-proANP and NT-proBNP outperformed ANP and BNP in predicting insulin resistance. Pro-NP glycosylation, which can impair NP and NT-proNP production in obesity, does not account for the diminished impact of ANP or BNP. Plasma concentrations of osteocrin, which competes for the NP clearance receptor (NPR-C) and potentially enhances NP bioactivity, was not associated with NPs, but did positively predict insulin resistance in females.

Conclusions

We find no evidence that increased clearance/degradation of NPs contributes to insulin resistance. Among the nine NP variants assessed, only NT-proANP and NT-proBNP independently predicted insulin resistance in both sexes. The impact of CNP on fat mass or insulin resistance was minor but significant in females. Lower concentrations of immunoreactive plasma ANP and BNP remains unexplained and requires closer study.

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引用次数: 0

Quantitative abnormalities in the β-region of the electrophoretic profile of serum proteins as predictive markers of monoclonality: Machine learning for monoclonality prediction

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.clinbiochem.2025.110892

Sara Cherkaoui, Slavka Penickova , Frederic Cotton

Objective of the study

To develop a machine learning algorithm aimed at predicting the presence of a monoclonal (M-) protein when the β-globulin fraction is elevated.

Materials and method

Patients were selected as part of the University Hospital Laboratory of Brussels routine diagnostic procedures from October 2021 to April 2022. Adult patients with serum protein electrophoresis showing elevated β1 and/or β2 fractions were included. The selection was done following strict exclusion criteria such as acute inflammation, iron deficiency anemia signs or supposed liver disease. To construct a predictive model for prediction of positive immunofixation (IFE) for monoclonality, the following factors were used: age, sex, β1 and β2 concentration (g/L), total proteins (g/L), IgA, IgM, IgG values (g/L) and hypogammaglobulinemia. The dataset underwent a random split, divided into a foundational training set (80%, 247 samples) and a foundational test set (20%, 62 samples). The training sets were subjected to five different algorithms: logistic regression, decision tree, random forest, gradient boosting, and support vector.

Results

309 patients were selected; 149 exhibited a negative IFE and 160 a positive IFE for monoclonality. The evaluation of the five tested models demonstrated very good performance, the chosen model was Random Forest for its high sensitivity (85%) and area under the receiver operating characteristic curve (91%).

Conclusion

An accurate algorithm was achieved for predicting the presence of M protein when the β-globulin fraction is elevated which enables early and improved diagnosis of monoclonal gammopathy.

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引用次数: 0

Oxidative stress and obesity are associated with endothelial dysfunction and subclinical atherosclerosis in adolescents

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-25 DOI: 10.1016/j.clinbiochem.2025.110889

Josiane Aparecida de Miranda , Warlley Rosa Cunha , Júlio César Moraes Lovisi , Carla Márcia Moreira Lanna , Lucas Cézar Pinheiro , Riccardo Lacchini , José Eduardo Tanus-Santos , Vanessa de Almeida Belo

Objectives

This study explores the relationship between obesity, endothelial dysfunction, and the critical role of oxidative stress biomarkers in subclinical atherosclerosis.

Design & methods

The study included 114 adolescents aged 12–17 years from Juiz de Fora, Brazil, divided into 40 individuals with obesity and 74 controls. Physical and biochemical assessments were conducted, including measurements of Brachial Flow-Mediated Dilation (BFMD), Carotid Intima-Media Thickness (IMT), and oxidative biomarkers such as nitrite, nitrate, and 8-isoprostane. Multiple regression analyses were used to evaluate associations between obesity, oxidative biomarkers, and endothelial function.

Results

Adolescents with obesity exhibited significantly reduced BFMD at 60 s (5.44 ± 2.31 % vs. 7.82 ± 2.07 % in controls; p < 0.05) and 90 s (5.27 ± 2.64 % vs. 7.93 ± 2.12 % in controls; p < 0.05). IMT was significantly higher in the group with obesity for both the right carotid artery (0.054 ± 0.005 cm vs. 0.047 ± 0.004 cm in controls; p < 0.05) and the left carotid artery (0.053 ± 0.005 cm vs. 0.047 ± 0.004 cm in controls; p < 0.05). Additionally, 8-isoprostane levels were higher in adolescents with obesity (49.75 ± 22.62 pg/mL vs. 42.36 ± 17.35 pg/mL in controls; p < 0.05), indicating increased oxidative stress. Nitrite levels were significantly lower in adolescents with obesity (42.98 ± 10.62 nM vs. 49.94 ± 17.71 nM in controls; p < 0.05). Additionally, nitrate levels were inversely associated with IMT in both the right (p = 0.01) carotid arteries in the multiple linear regression analyses.

Conclusions

The study highlights the association between obesity and early vascular changes in adolescents, evidenced by reduced BFMD, increased IMT, and altered oxidative stress biomarkers.

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引用次数: 0

Evaluation of an automated assay for eosinophil-derived neurotoxin in serum

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-25 DOI: 10.1016/j.clinbiochem.2025.110890

Carley Karsten , Theodore Stier , Christina Wood-Wentz , Carin Smith , Yifei K. Yang , Melissa Snyder

Introduction

Eosinophil-derived neurotoxin (EDN) is a promising biomarker for eosinophil activation during inflammatory responses. Here we evaluate the analytical performance of an automated fluorescence enzyme immunoassay for EDN in serum and explore its relationship with eosinophil counts in both healthy participants and those with eosinophilic conditions.

Materials and Methods

Paired serum samples were collected from individuals for whom a complete blood count with differential was ordered. EDN was measured using the ImmunoCAP EDN Assay Kit (research use only, Phadia AB / provided by Thermo Fisher Scientific) and 40 samples were also measured using an ELISA kit (research use only, ALPCO).

Results

The analytical measurement range of the ImmunoCAP assay was 2.6–200 µg/L. The imprecision across different EDN concentrations was ≤ 7.0 %. Stability and preanalytical requirements were determined. To minimize ex vivo degranulation and false elevation of EDN levels, serum should be removed from the cell pellet immediately after centrifugation. There was strong correlation for EDN measurements between ImmunoCAP and the comparative ELISA (r = 0.974), although a significant bias was observed. A 95th percentile reference range in 180 presumed healthy adults was calculated at 101 µg/L. Overall EDN was significantly higher in serum from patients with elevated circulating eosinophil counts (median = 120.0; P < 0.0001). However, individual patients may present with discordant presentation of eosinophil counts and EDN concentration.

Conclusions

Together these results demonstrate that the ImmunoCAP EDN Assay Kit can reliably measure EDN in serum and may be useful for the evaluation of patients with conditions associated with hypereosinophilia.

简介嗜酸性粒细胞衍生神经毒素（EDN）是炎症反应期间嗜酸性粒细胞活化的一种很有前景的生物标记物。在此，我们评估了血清中 EDN 自动荧光酶免疫分析仪的分析性能，并探讨了它与健康人和嗜酸性粒细胞病患者中嗜酸性粒细胞计数的关系：从需要进行全血细胞计数和差值检查的人群中采集配对血清样本。使用 ImmunoCAP EDN 分析试剂盒（仅供研究使用，Phadia AB / Thermo Fisher Scientific 提供）测量 EDN，同时使用 ELISA 试剂盒（仅供研究使用，ALPCO）测量 40 份样本：结果：ImmunoCAP 分析法的分析测量范围为 2.6-200 µg/L。不同浓度 EDN 的不精确度≤ 7.0%。确定了稳定性和分析前要求。为尽量减少体内脱颗粒和 EDN 水平的错误升高，离心后应立即从细胞团中去除血清。ImmunoCAP与对比ELISA的EDN测量结果有很强的相关性（r = 0.974），但也观察到了明显的偏差。在 180 名假定健康的成年人中计算出的第 95 百分位数参考范围为 101 µg/L。在循环嗜酸性粒细胞计数升高的患者血清中，总的 EDN 值明显较高（中位数 = 120.0；P < 0.0001）。然而，个别患者的嗜酸性粒细胞计数和 EDN 浓度可能不一致：这些结果表明，ImmunoCAP EDN 检测试剂盒能可靠地检测血清中的 EDN，可用于评估嗜酸性粒细胞过多相关疾病的患者。

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引用次数: 0

Assessing the predictive value of elevated triglycerides, triglyceride-glucose index (TyG), and TG/HDL ratios for cardiovascular disease and mortality during 20 years of follow-up: Tehran lipid and glucose study

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-23 DOI: 10.1016/j.clinbiochem.2025.110891

Shayesteh Khalili , Atieh Amouzegar , Seyed Sattar Dorost , Fereidoun Azizi , Aryan Salahi-Niri

Objectives

Cardiovascular disease (CVD) remains a leading cause of morbidity and mortality globally, influenced by a complex interplay of risk factors including lipid disorders and insulin resistance (IR). The triglyceride-glucose (TyG) index and the triglyceride to high-density lipoprotein cholesterol (TG/HDL) ratio have emerged as potential indicators for assessing cardiovascular risk. This study aimed to evaluate the predictive value of hypertriglyceridemia, the TyG index, and the TG/HDL ratio for mortality and CVD occurrence within an Iranian population.

Design and methods

Conducted within the Tehran Lipid and Glucose Study over 20 years, this research analyzed 7,117 participants to assess the association between these lipid biomarkers and CVD risk and mortality. Participants were stratified by their TyG and TG/HDL indices, with Cox proportional hazards models determining risk ratios across three adjusted models considering various demographic and clinical variables.

Results

The study found significant associations between elevated triglycerides, TyG, and TG/HDL levels with increased risks of mortality and CVD during the 20-year follow-up. Specifically, the hazard ratios for CVD events were notably significant in the second triglyceride group (150–250 mg/dL), with a hazard ratio of 1.36 (1.19–1.55) in both Model 1 and Model 2, and in the third group (250–400 mg/dL), with ratios of 1.88 (1.63–2.17) in Model 1, 1.90 (1.65–2.19) in Model 2, and 1.44 (1.24–1.67) in Model 3.

Conclusion

Hypertriglyceridemia, the TyG index, and the TG/HDL ratio are easily calculable and clinically relevant markers for cardiovascular risk assessment. Their integration into routine health evaluations could facilitate early detection and management of at-risk individuals, potentially reducing the incidence and impact of CVD within the community.

{"title":"Assessing the predictive value of elevated triglycerides, triglyceride-glucose index (TyG), and TG/HDL ratios for cardiovascular disease and mortality during 20 years of follow-up: Tehran lipid and glucose study","authors":"Shayesteh Khalili , Atieh Amouzegar , Seyed Sattar Dorost , Fereidoun Azizi , Aryan Salahi-Niri","doi":"10.1016/j.clinbiochem.2025.110891","DOIUrl":"10.1016/j.clinbiochem.2025.110891","url":null,"abstract":"<div><h3>Objectives</h3><div>Cardiovascular disease (CVD) remains a leading cause of morbidity and mortality globally, influenced by a complex interplay of risk factors including lipid disorders and insulin resistance (IR). The triglyceride-glucose (TyG) index and the triglyceride to high-density lipoprotein cholesterol (TG/HDL) ratio have emerged as potential indicators for assessing cardiovascular risk. This study aimed to evaluate the predictive value of hypertriglyceridemia, the TyG index, and the TG/HDL ratio for mortality and CVD occurrence within an Iranian population.</div></div><div><h3>Design and methods</h3><div>Conducted within the Tehran Lipid and Glucose Study over 20 years, this research analyzed 7,117 participants to assess the association between these lipid biomarkers and CVD risk and mortality. Participants were stratified by their TyG and TG/HDL indices, with Cox proportional hazards models determining risk ratios across three adjusted models considering various demographic and clinical variables.</div></div><div><h3>Results</h3><div>The study found significant associations between elevated triglycerides, TyG, and TG/HDL levels with increased risks of mortality and CVD during the 20-year follow-up. Specifically, the hazard ratios for CVD events were notably significant in the second triglyceride group (150–250 mg/dL), with a hazard ratio of 1.36 (1.19–1.55) in both Model 1 and Model 2, and in the third group (250–400 mg/dL), with ratios of 1.88 (1.63–2.17) in Model 1, 1.90 (1.65–2.19) in Model 2, and 1.44 (1.24–1.67) in Model 3.</div></div><div><h3>Conclusion</h3><div>Hypertriglyceridemia, the TyG index, and the TG/HDL ratio are easily calculable and clinically relevant markers for cardiovascular risk assessment. Their integration into routine health evaluations could facilitate early detection and management of at-risk individuals, potentially reducing the incidence and impact of CVD within the community.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"136 ","pages":"Article 110891"},"PeriodicalIF":2.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of the combination of high fluorescence cells and tumor markers for the diagnosis of malignant pleural effusions

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-21 DOI: 10.1016/j.clinbiochem.2025.110888

Elisa Nuez-Zaragoza , Indira Bhambi-Blanco , Mònica Vidal-Pla , Isabel Aparicio-Calvente , M. Rosa Escoda-Giralt , Joana Gallardo-Campos , Joan C. Ferreres , Luis Frisancho , Laia Mas-Maresma , Patricia Aguilera-Fernández , Sonia Marco-Continente , Marina Sierra-Boada , Pablo Andreu-Cobo , Miquel Gallego , Jaume Trapé , Vicente Aguadero

Background

New diagnostic tools have emerged to assist the traditional diagnosis of malignant pleural effusion (MPE), such as high fluorescence cells (HFc) and tumor markers (TMs), determined by clinical laboratory automated pleural fluid workup. This study aimed to evaluate the diagnostic ability of the combination of HFc and TMs for diagnosing MPE.

Methods

We recruited hospitalized patients with pleural effusion at Parc Taulí University Hospital. We collected and analyzed pleural fluid and serum samples in the clinical laboratory, and we sent a sample of pleural fluid to the Pathology Department for cytology workup. We determined the pleural fluid cell count by Sysmex XN-10 and assessed TMs (CEA, CA19.9, and CA15.3) using the ECLIA Cobas e801 Roche in both pleural fluid and serum samples. We established the final MPE diagnosis based on positive cytology and/or positive pleural biopsy. We classified patients based on these final diagnoses and conducted a comparison between variables, along with multivariate logistic regression.

Results

The study included 316 pleural effusions from 221 patients recruited. Multivariate logistic regression indicated the most significant predictor variables for MPE were CA15.3 in serum, CEA ratio, and HFc. We calculated two different models: one excluding HFc and one including it, with the latter displaying superior diagnostic ability (area under the curve 0.91). This model could identify 100 % of MPE cases with 30 % specificity at low cut-offs, and higher values could help identify 60 % of MPE cases with 100 % specificity.

Conclusions

Per our findings, this model has high diagnostic performance and could serve as a swift, automated, dependable, non-invasive tool for MPE detection.

{"title":"Utility of the combination of high fluorescence cells and tumor markers for the diagnosis of malignant pleural effusions","authors":"Elisa Nuez-Zaragoza , Indira Bhambi-Blanco , Mònica Vidal-Pla , Isabel Aparicio-Calvente , M. Rosa Escoda-Giralt , Joana Gallardo-Campos , Joan C. Ferreres , Luis Frisancho , Laia Mas-Maresma , Patricia Aguilera-Fernández , Sonia Marco-Continente , Marina Sierra-Boada , Pablo Andreu-Cobo , Miquel Gallego , Jaume Trapé , Vicente Aguadero","doi":"10.1016/j.clinbiochem.2025.110888","DOIUrl":"10.1016/j.clinbiochem.2025.110888","url":null,"abstract":"<div><h3>Background</h3><div>New diagnostic tools have emerged to assist the traditional diagnosis of malignant pleural effusion (MPE), such as high fluorescence cells (HFc) and tumor markers (TMs), determined by clinical laboratory automated pleural fluid workup. This study aimed to evaluate the diagnostic ability of the combination of HFc and TMs for diagnosing MPE.</div></div><div><h3>Methods</h3><div>We recruited hospitalized patients with pleural effusion at Parc Taulí University Hospital. We collected and analyzed pleural fluid and serum samples in the clinical laboratory, and we sent a sample of pleural fluid to the Pathology Department for cytology workup. We determined the pleural fluid cell count by Sysmex XN-10 and assessed TMs (CEA, CA19.9, and CA15.3) using the ECLIA Cobas e801 Roche in both pleural fluid and serum samples. We established the final MPE diagnosis based on positive cytology and/or positive pleural biopsy. We classified patients based on these final diagnoses and conducted a comparison between variables, along with multivariate logistic regression.</div></div><div><h3>Results</h3><div>The study included 316 pleural effusions from 221 patients recruited. Multivariate logistic regression indicated the most significant predictor variables for MPE were CA15.3 in serum, CEA ratio, and HFc. We calculated two different models: one excluding HFc and one including it, with the latter displaying superior diagnostic ability (area under the curve 0.91). This model could identify 100 % of MPE cases with 30 % specificity at low cut-offs, and higher values could help identify 60 % of MPE cases with 100 % specificity.</div></div><div><h3>Conclusions</h3><div>Per our findings, this model has high diagnostic performance and could serve as a swift, automated, dependable, non-invasive tool for MPE detection.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"136 ","pages":"Article 110888"},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical significance of fecal Syndecan-2 gene methylation combined with blood tumor abnormal protein detection in the diagnosis of colorectal cancer and precancerous lesions 粪便Syndecan-2基因甲基化联合血液肿瘤异常蛋白检测在结直肠癌及癌前病变诊断中的临床意义

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-18 DOI: 10.1016/j.clinbiochem.2025.110887

Xuanjun Liu , Guowei Zhao , Weixu Mao , Qigang Li , Juan Liao , Gan He

Objective

To investigate the clinical significance of fecal Syndecan-2 (SDC2) gene methylation combined with blood tumor abnormal protein (TAP) detection for the diagnosis of colorectal cancer (CRC) and its precancerous lesions.

Methods

A retrospective study was conducted to collect patients diagnosed with CRC or colorectal adenoma (Ade) from March 2020 to March 2023, and healthy people (Nor) without any gastrointestinal diseases during the same period as the control group. All participants underwent the fecal SDC2 gene methylation test, blood TAP test and fecal occult blood test (FOBT). The differences in the positivity rates of each index were compared, receiver operator characteristic curves were plotted and the area under the curve (AUC) was calculated to evaluate the diagnostic effects of different testing methods on CRC and its precancerous lesions.

Results

A total of 146 individuals were included in the study, including 69 CRC patients, 47 patients with Ade and 30 healthy individuals. The results showed that, SDC2, TAP and the combined assay had high comprehensive diagnostic efficacy for the diagnosis of CRC, but there was no significant difference between the three methods in terms of AUC, sensitivity, and specificity. However, for Ade, the combined detection was statistically significant, with a high AUC (0.905), high sensitivity (95.7%), and high specificity (86.7%).

Conclusion

Fecal SDC2 gene methylation combined with blood TAP detection is an effective noninvasive screening and diagnostic method to enhance the early detection and treatment of CRC precancerous lesions, such as Ade, thereby reducing the incidence and mortality of CRC.

目的：探讨粪便Syndecan-2 （SDC2）基因甲基化联合血液肿瘤异常蛋白（TAP）检测在结直肠癌（CRC）及其癌前病变诊断中的临床意义。方法：回顾性研究收集2020年3月至2023年3月诊断为结直肠癌或结直肠腺瘤（Ade）的患者，以及同期无胃肠道疾病的健康人群（Nor）作为对照组。所有参与者都进行了粪便SDC2基因甲基化测试、血液TAP测试和粪便潜血测试（FOBT）。比较各指标阳性率的差异，绘制受试者操作者特征曲线，计算曲线下面积（AUC），评价不同检测方法对结直肠癌及其癌前病变的诊断效果。结果：共纳入146例，其中CRC患者69例，Ade患者47例，健康个体30例。结果显示，SDC2、TAP及联合检测对结直肠癌的诊断具有较高的综合诊断效能，但三种方法在AUC、敏感性、特异性方面无显著差异。然而，对于Ade，联合检测具有统计学意义，具有高AUC(0.905)，高灵敏度（95.7%）和高特异性（86.7%）。结论：粪便SDC2基因甲基化联合血液TAP检测是一种有效的无创筛查和诊断方法，可提高对Ade等结直肠癌癌前病变的早期发现和治疗，从而降低结直肠癌的发病率和死亡率。

{"title":"Clinical significance of fecal Syndecan-2 gene methylation combined with blood tumor abnormal protein detection in the diagnosis of colorectal cancer and precancerous lesions","authors":"Xuanjun Liu , Guowei Zhao , Weixu Mao , Qigang Li , Juan Liao , Gan He","doi":"10.1016/j.clinbiochem.2025.110887","DOIUrl":"10.1016/j.clinbiochem.2025.110887","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the clinical significance of fecal Syndecan-2 <em>(SDC2)</em> gene methylation combined with blood tumor abnormal protein (TAP) detection for the diagnosis of colorectal cancer (CRC) and its precancerous lesions.</div></div><div><h3>Methods</h3><div>A retrospective study was conducted to collect patients diagnosed with CRC or colorectal adenoma (Ade) from March 2020 to March 2023, and healthy people (Nor) without any gastrointestinal diseases during the same period as the control group. All participants underwent the fecal <em>SDC2</em> gene methylation test, blood TAP test and fecal occult blood test (FOBT). The differences in the positivity rates of each index were compared, receiver operator characteristic curves were plotted and the area under the curve (AUC) was calculated to evaluate the diagnostic effects of different testing methods on CRC and its precancerous lesions.</div></div><div><h3>Results</h3><div>A total of 146 individuals were included in the study, including 69 CRC patients, 47 patients with Ade and 30 healthy individuals. The results showed that, SDC2, TAP and the combined assay had high comprehensive diagnostic efficacy for the diagnosis of CRC, but there was no significant difference between the three methods in terms of AUC, sensitivity, and specificity. However, for Ade, the combined detection was statistically significant, with a high AUC (0.905), high sensitivity (95.7%), and high specificity (86.7%).</div></div><div><h3>Conclusion</h3><div>Fecal <em>SDC2</em> gene methylation combined with blood TAP detection is an effective noninvasive screening and diagnostic method to enhance the early detection and treatment of CRC precancerous lesions, such as Ade, thereby reducing the incidence and mortality of CRC.</div></div>","PeriodicalId":10172,"journal":{"name":"Clinical biochemistry","volume":"136 ","pages":"Article 110887"},"PeriodicalIF":2.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

43 G > T polymorphism in the sucrase-isomaltase gene in the Chinese population prevents the glucose-lowering effect of acarbose 43 G > 中国人群蔗糖-异麦芽糖酶基因的T多态性阻止了阿卡波糖的降血糖作用。

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry

Pub Date : 2025-01-11 DOI: 10.1016/j.clinbiochem.2025.110875

Junyao Huang , Yan Chen , Maolian Zhong , Yan Liu , Xing Wang , Wenqiang Xiong , Xiaodan Chen , Xiaoyi Yi , Yuting Liu , Hong Zhang

Background

Acarbose is an α-glucosidase inhibitor widely used clinically for its significant hypoglycemic effect, albeit with inter-individual variations in response. The sucrase-isomaltase (SI) enzyme is the primary target of acarbose. This study aims to investigate the impact of genetic polymorphisms in the SI gene on the pharmacodynamics of acarbose.

Methods

The Illumina sequencing platform and variation-related databases were employed to analyze probable gene polymorphism sites of SI. Based on the SI polymorphism sites, Chinese subjects (n = 66) were categorized into the wild-type homozygous group (Group A) and the heterozygous variant group (Group B, SI 43 G > T). The validated hexokinase method was utilized to determine glucose concentrations in participants’ serum samples. The differences in blood glucose concentration reduction and pharmacodynamic parameters peak concentration (C_max) and area under the curve (AUC_0-2h) after administering the same dose of acarbose were analyzed between the two groups of subjects.

Results

Our results showed that the mean changes in glucose C_max, AUC_0-2h, maximum increase, and maximum decrease in Group B were each lower by 67.66 %, 63.05 %, 53.17 %, and 50 % compared to Group A (all p < 0.05).

Conclusions

These data suggested that genetic polymorphism of the SI gene can significantly influence the hypoglycemic efficacy of acarbose, and the polymorphism of SI is associated with individual differences in clinical treatment outcomes.

背景：阿卡波糖是一种α-葡萄糖苷酶抑制剂，因其显著的降糖作用而广泛应用于临床，但个体间的降糖反应存在差异。蔗糖-异麦芽糖酶（SI）酶是阿卡波糖的主要靶标。本研究旨在探讨SI基因遗传多态性对阿卡波糖药效学的影响。方法：利用Illumina测序平台和变异相关数据库对SI可能的基因多态性位点进行分析。根据SI多态性位点，将中国受试者（n = 66）分为野生型纯合子组（A组）和杂合子变异组（B组，SI 43 G > T）。采用已验证的己糖激酶法测定受试者血清样品中的葡萄糖浓度。分析两组受试者给予相同剂量阿卡波糖后血糖浓度降低及药效学参数峰浓度（Cmax）和曲线下面积（AUC0-2h）的差异。结果：我们的研究结果显示，与A组相比，B组血糖Cmax、AUC0-2h、最大增幅和最大降幅的平均变化分别降低67.66 %、63.05 %、53.17 %和50 % （p均为 ）。结论：这些数据提示SI基因的遗传多态性可显著影响阿卡波糖的降糖效果，SI基因的多态性与临床治疗结果的个体差异有关。

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引用次数: 0