Clinical and laboratory haematology最新文献_第10页

Letter from America: the end of civilization as we know it? 美国来信:我们所知的文明的终结?

Clinical and laboratory haematology

Pub Date : 1995-09-01

S A Bentley

引用次数: 0

Low serum erythropoietin--a strong diagnostic criterion of primary polycythaemia even at normal haemoglobin levels. 低血清促红细胞生成素-原发性红细胞增多症的一个强有力的诊断标准，即使在正常血红蛋白水平。

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01239.X

M. Messinezy, N. Westwood, S. Woodcock, R. Strong, Thomas C. Pearson

Estimations of serum erythropoietin level by enzyme immunoassay (EIA) kit were made in 42 patients with primary polycythaemia, and in a comparison group consisting of 41 patients with secondary polycythaemia and 47 patients with idiopathic erythrocytosis. The majority of patients with primary polycythaemia were undergoing treatment by venesection and therefore had Hb levels in the normal range at the time of erythropoietin estimation. In primary polycythaemia, 64% of the first samples taken from each patient were below the reference range for serum erythropoietin in normal individuals. When two samples were taken from each patient 72% had low values in one or both samples. In the comparison group, analysis of those patients who had two samples taken showed only one individual with secondary polycythaemia and none with idiopathic erythrocytosis who had a serum erythropoietin level below the reference range. The finding of low serum erythropoietin in patients with primary polycythaemia, even when Hb levels are normal due to venesection, is of high diagnostic specificity (few false positive results) and useful diagnostic sensitivity (28% false negative results). It is proposed that future diagnostic criteria of primary polycythaemia should include the finding of a serum erythropoietin level below the lower limit of normal in at least one of two serum samples taken on different occasions.

采用酶免疫测定(EIA)试剂盒对42例原发性红细胞增多症患者和41例继发性红细胞增多症患者及47例特发性红细胞增多症患者进行血清促红细胞生成素水平测定。大多数原发性多红细胞血症患者正在接受静脉切除治疗，因此在促红细胞生成素估计时Hb水平在正常范围内。在原发性红细胞增多症中，从每个患者身上采集的第一批样本中有64%低于正常人血清促红细胞生成素的参考范围。当从每位患者中抽取两个样本时，其中一个或两个样本中72%的值较低。在对照组中，对采集了两个样本的患者进行分析，结果显示只有一个人患有继发性红细胞增多症，而没有一个人患有特发性红细胞增多症，其血清促红细胞生成素水平低于参考范围。原发性多红细胞血症患者血清促红细胞生成素水平低，即使由于静脉切除Hb水平正常，也具有很高的诊断特异性(很少有假阳性结果)和有用的诊断敏感性(28%假阴性结果)。建议将来原发性红细胞增多症的诊断标准应包括在不同场合采集的两份血清样本中至少有一份血清促红细胞生成素水平低于正常下限。

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引用次数: 61

Acute non-lymphoblastic leukaemia with t(16;21): case report with a review of the literature. 急性非淋巴细胞白血病伴t(16;21): 1例报告并文献复习。

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01244.X

M. Hiyoshi, K. Koh, T. Yamane, N. Tatsumi

A rare case of acute non-lymphoblastic leukaemia with chromosomal t(16;21)(p11;q22) translocation was studied at the molecular level. Most of the previous reports about the translocation have been described at a karyotypic level. Blast cells of this patient expressed the TLS-ERG chimeric mRNA. The clinical, morphological, karyotypic, and immunohistochemical aspects of this leukaemia are also presented with a review of the literature.

在分子水平上研究了一例罕见的急性非淋巴细胞白血病伴染色体t(16;21)(p11;q22)易位。大多数关于易位的先前报告都是在核型水平上描述的。该患者的母细胞表达了TLS-ERG嵌合mRNA。临床，形态学，核型和免疫组织化学方面的这种白血病也提出了文献综述。

引用次数: 12

Letter from America: the end of civilization as we know it? 美国来信:我们所知的文明的终结?

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01246.X

S. Bentley

引用次数: 0

Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant. 重组凝血活酶抑制法检测狼疮抗凝血药物。

Clinical and laboratory haematology

Pub Date : 1995-09-01

E Zanon, M A Saracino, P Simioni, L Scarano, B Girolami, A Girolami

Tissue thromboplastin inhibition assay (TTI) is a sensitive test for lupus anticoagulant (LA). We have performed TTI in 12 LA positive patients using a new recombinant human tissue factor (Innovin, IN) and compared it with Thromborel S (TH), a commonly used human placenta thromboplastin. The effect of using two different dilutions of each thromboplastin (1:10 & 1:26) was investigated. A 1:26 dilution discriminated better than the 1:10 and this was more evident for Innovin. The mean value obtained with a 1:26 IN dilution was statistically different from that observed with TH at the same dilution. Furthermore, when PT and TTI ratios were considered, differences were statistically significant and seemed to increase depending on thromboplastin dilutions. When we used IN at 1:26 all LA positive patients had a value > 1.2. Then we compared TTI at a 1:26 dilution with dilute Russell's Viper Venom Time (dRVVT) in 50 consecutive patients with suspected lupus anticoagulant not treated with warfarin or heparin. In these patients the diagnosis of lupus anticoagulant was carried out using dilute APTT mixing studies and a platelet neutralization procedure: four out of 50 patients thus tested were LA positive. When dRVVT or TTI-I 1:26 were used, five patients were positive for lupus anticoagulant. Innovin showed similar sensitivity of dRVVT for detection of lupus anticoagulant. It is likely that higher dilutions of thromboplastins could further improve the specificity of this method.

组织凝血活素抑制试验(TTI)是一种敏感的红斑狼疮抗凝剂(LA)检测方法。我们使用一种新的重组人组织因子(Innovin, in)对12例LA阳性患者进行TTI，并将其与一种常用的人胎盘凝血活素(Thromborel S, TH)进行比较。研究了使用两种不同稀释度的凝血活素(1:10和1:26)的效果。1:26的稀释比1:10更好，这在Innovin上更为明显。用1:26 IN稀释得到的平均值与用相同稀释度的TH观察到的平均值有统计学差异。此外，当考虑PT和TTI比率时，差异具有统计学意义，并且似乎随着凝血活蛋白稀释度的增加而增加。当我们在1:26使用IN时，所有LA阳性患者的值都大于1.2。然后，我们比较了50例连续未使用华法林或肝素治疗的疑似狼疮抗凝剂患者1:26稀释的TTI和稀释的罗素毒蛇毒液时间(dRVVT)。在这些患者中，使用稀释APTT混合研究和血小板中和程序进行狼疮抗凝诊断:50例患者中有4例LA阳性。采用dRVVT或TTI-I 1:26检测时，5例患者狼疮抗凝血阳性。Innovin与dRVVT检测狼疮抗凝血药的敏感性相似。可能更高稀释度的凝血酶可以进一步提高该方法的特异性。

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引用次数: 0

Evaluation of monocyte counting with two automated instruments by the use of CD 14-specific immunomagnetic Dynabeads. 使用cd14特异性免疫磁头对两种自动仪器单核细胞计数的评价。

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01241.X

L. Theodorsen

Accurate determination of monocytes can be a problem with automated haematological analysers as well as with visual microscopy. We have compared monocyte counts from our two routine automated instruments, Technicon H*1 and Sysmex NE-1500. In the NE-1500 the monocyte counts appear to increase with increasing concentrations of neutrophils, compared with the H*1 results. The accuracy of the monocyte counts was assessed using Dynabeads M-450 CD14. These uniform magnetizable polystyrene beads coated with monoclonal antibody specific for CD14 membrane antigen isolated monocytes from the blood samples. The influence of this depletion on the monocyte counts and histograms from the two instruments and by visual microscopy were compared. On average 90% of the monocytes counted by visual microscopy were removed from six blood samples. In the same samples the beads removed an average of 75% of cells identified as monocytes by the H*1 but only 49% of those identified as monocytes by the NE-1500. These Dynabeads can be a useful tool in evaluating monocyte identification in different haematological situations and for manufacturers developing technology for automated leucocyte differential counting.

准确测定单核细胞可能是一个问题与自动血液学分析仪以及视觉显微镜。我们比较了我们的两个常规自动化仪器，Technicon H*1和Sysmex NE-1500的单核细胞计数。与H*1结果相比，NE-1500单核细胞计数随着中性粒细胞浓度的增加而增加。使用dynabead M-450 CD14评估单核细胞计数的准确性。这些涂有CD14膜抗原特异性单克隆抗体的均匀磁化聚苯乙烯珠从血液样本中分离出单核细胞。比较了这两种仪器和视觉显微镜对单核细胞计数和直方图的影响。平均90%的单核细胞通过视觉显微镜计数从6个血液样本中被去除。在相同的样品中，珠粒平均去除75%被H*1鉴定为单核细胞的细胞，但仅去除49%被NE-1500鉴定为单核细胞的细胞。这些dynabheads可以在不同血液学情况下评估单核细胞鉴定，并为制造商开发自动白细胞鉴别计数技术提供有用的工具。

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引用次数: 11

An unusual case of hypersomnolence syndrome. 嗜睡症的罕见病例。

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/j.1365-2257.1995.tb01245.x

M. Gandhi, J. E. Chandler

Hypersomnolence syndrome has been well described in patients with acute lymphoblastic leukaemia (ALL) following cranial irradiation. It is most frequently seen in children. In adults it is clinically very mild. We describe a case of a particularly severe and prolonged post radiation hypersomnolence syndrome in an adult with ALL. The hypersomnolence syndrome is discussed in the context of side effects of central nervous system directed therapy in elderly patients with ALL.

嗜睡综合征已经很好地描述了急性淋巴细胞白血病(ALL)患者在颅脑照射后的症状。最常见于儿童。成人临床症状非常轻微。我们描述了一个特别严重和长期的辐射后嗜睡综合征在一个成年人与ALL。嗜睡综合征在中枢神经系统指导治疗的副作用的背景下进行了讨论。

引用次数: 0

Risk factors for arterial disease: laboratory assessment 动脉疾病的危险因素:实验室评估

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01252.X

G. Lowe

引用次数: 0

Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant. 重组凝血活酶抑制法检测狼疮抗凝血药物。

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01242.X

E. Zanon, M. A. Saracino, P. Simioni, L. Scarano, B. Girolami, A. Girolami

Tissue thromboplastin inhibition assay (TTI) is a sensitive test for lupus anticoagulant (LA). We have performed TTI in 12 LA positive patients using a new recombinant human tissue factor (Innovin, IN) and compared it with Thromborel S (TH), a commonly used human placenta thromboplastin. The effect of using two different dilutions of each thromboplastin (1:10 & 1:26) was investigated. A 1:26 dilution discriminated better than the 1:10 and this was more evident for Innovin. The mean value obtained with a 1:26 IN dilution was statistically different from that observed with TH at the same dilution. Furthermore, when PT and TTI ratios were considered, differences were statistically significant and seemed to increase depending on thromboplastin dilutions. When we used IN at 1:26 all LA positive patients had a value > 1.2. Then we compared TTI at a 1:26 dilution with dilute Russell's Viper Venom Time (dRVVT) in 50 consecutive patients with suspected lupus anticoagulant not treated with warfarin or heparin. In these patients the diagnosis of lupus anticoagulant was carried out using dilute APTT mixing studies and a platelet neutralization procedure: four out of 50 patients thus tested were LA positive. When dRVVT or TTI-I 1:26 were used, five patients were positive for lupus anticoagulant. Innovin showed similar sensitivity of dRVVT for detection of lupus anticoagulant. It is likely that higher dilutions of thromboplastins could further improve the specificity of this method.

组织凝血活素抑制试验(TTI)是一种敏感的红斑狼疮抗凝剂(LA)检测方法。我们使用一种新的重组人组织因子(Innovin, in)对12例LA阳性患者进行TTI，并将其与一种常用的人胎盘凝血活素(Thromborel S, TH)进行比较。研究了使用两种不同稀释度的凝血活素(1:10和1:26)的效果。1:26的稀释比1:10更好，这在Innovin上更为明显。用1:26 IN稀释得到的平均值与用相同稀释度的TH观察到的平均值有统计学差异。此外，当考虑PT和TTI比率时，差异具有统计学意义，并且似乎随着凝血活蛋白稀释度的增加而增加。当我们在1:26使用IN时，所有LA阳性患者的值都大于1.2。然后，我们比较了50例连续未使用华法林或肝素治疗的疑似狼疮抗凝剂患者1:26稀释的TTI和稀释的罗素毒蛇毒液时间(dRVVT)。在这些患者中，使用稀释APTT混合研究和血小板中和程序进行狼疮抗凝诊断:50例患者中有4例LA阳性。采用dRVVT或TTI-I 1:26检测时，5例患者狼疮抗凝血阳性。Innovin与dRVVT检测狼疮抗凝血药的敏感性相似。可能更高稀释度的凝血酶可以进一步提高该方法的特异性。

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引用次数: 3

Molecular biology of blood groups: applications in prenatal blood grouping 血型分子生物学:在产前血型鉴定中的应用

Clinical and laboratory haematology

Pub Date : 1995-09-01 DOI: 10.1111/j.1365-2257.1995.tb01255.x

D. Anstee

引用次数: 0