Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.013
Yanze Sun, Yongqiang Yang, J. Qian
Objective To evaluate and predict the pelvic dose by analyzing two pelvic contour definitions and identify the influencing factors of the pelvic dose in postoperative IMRT for cervical cancer, aiming to provide reference for postoperative pelvis-sparing IMRT for cervical cancer. Methods Sixty cervical cancer patients receiving postoperative IMRT with unrestricted pelvic dose were selected. Two sets of pelvic contours (pelvic anatomy and pelvic Mell) were delineated as per the anatomical and Mell methods. The dose relationship between two methods was analyzed after redesigning the treatment plan by limiting dose of pelvic anatomy. The correlation analysis was performed by Pearson’s correlation method. The factors affecting the pelvic anatomy dose were identified by Logistic multivariate regression analysis and a dose prediction model was subsequently established. Results The volumes of pelvic anatomy and pelvic Mell were 925.82 cm3 and 1141.20 cm3(P=0.000). There was a significant correlation between them (r>0.622, P=0.000). The dose of pelvic anatomy was significantly higher than that of pelvic Mell. The relationship of V10, V20 and V30 between them was y=-8+ 1.01x, y=-13+ 1.05x and y=-4+ 0.9x, respectively. The dose limits of pelvic Mell recommended by literatures(V10<90%, V20<75%, V30<60%) were translated into V10<97%, V20<83% and V30<70%, respectively. The pelvic anatomy dose was significantly reduced after dose limiting. The V10, V20, V30 and Dmean were significantly decreased by 3.64%, 12.69%, 12.02% and 6.93%(P=0.000, 0.000, 0.000), respectively. Multivariate analysis showed that the overlapping volume of pelvic anatomy within PTV was an independent influencing factor of pelvic anatomy dose (P<0.05). Patients with a relative overlapping volume of less than 18% could easily meet the dose limiting requirement. Conclusions Both two pelvic contour definitions can be applied in postoperative pelvis-sparing IMRT for cervical cancer. Use of pelvic dose limiting can significantly reduce the IMRT dose. The overlapping volume of the pelvis within PTV is an independent influencing factor of pelvic dose. Patients whose overlapping volume within the PTV relative to pelvis is less than 18% can easily meet the dose limiting requirement. Key words: Cervical cancer/intensity-modulated radiotherapy; Pelvis; Dosimetry
{"title":"Evaluation and prediction of pelvic dose in postoperative IMRT for cervical cancer","authors":"Yanze Sun, Yongqiang Yang, J. Qian","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.013","url":null,"abstract":"Objective \u0000To evaluate and predict the pelvic dose by analyzing two pelvic contour definitions and identify the influencing factors of the pelvic dose in postoperative IMRT for cervical cancer, aiming to provide reference for postoperative pelvis-sparing IMRT for cervical cancer. \u0000 \u0000 \u0000Methods \u0000Sixty cervical cancer patients receiving postoperative IMRT with unrestricted pelvic dose were selected. Two sets of pelvic contours (pelvic anatomy and pelvic Mell) were delineated as per the anatomical and Mell methods. The dose relationship between two methods was analyzed after redesigning the treatment plan by limiting dose of pelvic anatomy. The correlation analysis was performed by Pearson’s correlation method. The factors affecting the pelvic anatomy dose were identified by Logistic multivariate regression analysis and a dose prediction model was subsequently established. \u0000 \u0000 \u0000Results \u0000The volumes of pelvic anatomy and pelvic Mell were 925.82 cm3 and 1141.20 cm3(P=0.000). There was a significant correlation between them (r>0.622, P=0.000). The dose of pelvic anatomy was significantly higher than that of pelvic Mell. The relationship of V10, V20 and V30 between them was y=-8+ 1.01x, y=-13+ 1.05x and y=-4+ 0.9x, respectively. The dose limits of pelvic Mell recommended by literatures(V10<90%, V20<75%, V30<60%) were translated into V10<97%, V20<83% and V30<70%, respectively. The pelvic anatomy dose was significantly reduced after dose limiting. The V10, V20, V30 and Dmean were significantly decreased by 3.64%, 12.69%, 12.02% and 6.93%(P=0.000, 0.000, 0.000), respectively. Multivariate analysis showed that the overlapping volume of pelvic anatomy within PTV was an independent influencing factor of pelvic anatomy dose (P<0.05). Patients with a relative overlapping volume of less than 18% could easily meet the dose limiting requirement. \u0000 \u0000 \u0000Conclusions \u0000Both two pelvic contour definitions can be applied in postoperative pelvis-sparing IMRT for cervical cancer. Use of pelvic dose limiting can significantly reduce the IMRT dose. The overlapping volume of the pelvis within PTV is an independent influencing factor of pelvic dose. Patients whose overlapping volume within the PTV relative to pelvis is less than 18% can easily meet the dose limiting requirement. \u0000 \u0000 \u0000Key words: \u0000Cervical cancer/intensity-modulated radiotherapy; Pelvis; Dosimetry","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"136-140"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43629969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.015
Zekun Wang
Perineural invasion is a unique biological behavior of tumors via nerve dissemination and metastasis, which is intimately correlated with the recurrence, metastasis and prognosis. With the deepening research, the clinical significance of perineural invasion has been widely recognized. However, due to the low incidence of head and neck neoplasms and the varying incidence of perineural invasion in different anatomical sites and pathological types, high-level evidence-based medical evidence is still lacking. Currently, the pathological mechanism of perineural invasion has not been clearly clarified. No specific treatment has been available for perineural invasion. Therefore, the treatment of perineural invasion of head and neck tumors is an enormous challenge for clinicians. In this article, the current status of clinical diagnosis and treatment of perineural invasion in head and neck malignancies was reviewed. Key words: Head and neck neoplasm; Perineural invasion; Diagnosis and treatment
{"title":"Clinical diagnosis and treatment of perineural invasion of head and neck malignant tumors","authors":"Zekun Wang","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.015","url":null,"abstract":"Perineural invasion is a unique biological behavior of tumors via nerve dissemination and metastasis, which is intimately correlated with the recurrence, metastasis and prognosis. With the deepening research, the clinical significance of perineural invasion has been widely recognized. However, due to the low incidence of head and neck neoplasms and the varying incidence of perineural invasion in different anatomical sites and pathological types, high-level evidence-based medical evidence is still lacking. Currently, the pathological mechanism of perineural invasion has not been clearly clarified. No specific treatment has been available for perineural invasion. Therefore, the treatment of perineural invasion of head and neck tumors is an enormous challenge for clinicians. In this article, the current status of clinical diagnosis and treatment of perineural invasion in head and neck malignancies was reviewed. \u0000 \u0000 \u0000Key words: \u0000Head and neck neoplasm; Perineural invasion; Diagnosis and treatment","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"146-149"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46875097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.007
Jie Wang, Miao Zhang, Gaiyan Li, Xintong Lyu, Q. Qiao
Objective To explore the mechanism of the role of Salubrinal in regulating the radiation-induced apoptosis of oral cancer cells. Methods Radioresistant KBR cell line was constructed (4 Gy per fraction, every 7-10 d for 4 times). The radiosensitivity of oral cancer cells after Salubrinal pretreatment was measured by colony formation assay. The expression levels of NF-κB-HIF-1α signaling pathway and apoptosis biomarker cleaved PARP in oral cancer cells were measured by Western blot. The apoptosis rate was detected by Annexin V, PI staining and flow cytometry. Results Colony formation assay demonstrated that Salubrinal increased the radiosensitivity of oral cancer cells. The radiosensitization ratios of KB and KBR cells were 1.19 and 1.24. Western blot revealed that the activation of NF-κB-HIF-1α was time-dependent in the radiation-induced oral cancer cells, whereas Salubrinal inhibited the radiation-induced abnormal activation. In addition, Salubrinal increased the expression of apoptosis biomarker cleaved PARP and apoptosis index in radiation-induced oral cancer cells, whereas TNF-α, an activator of NF-κB, reversed the effect, suggesting that Salubrinal increased the apoptosis of radiation-induced oral cancer cells by suppressing the activation of NF-κB. Pretreatment of NF-κB inhibitor Bay11-7082 also increased the cell apoptosis. The expression levels of cleaved PARP of KB and KBR cell lines in the Bay11-7082+ IR group were 2.67±0.26 and 1.91±0.17, significantly higher compared with 2.1±0.16 and 1.44±0.15 in the IR group (both P<0.05). Conclusion Salubrinal can aggravate the apoptosis of radiation-induced oral cancer cells by inhibiting the radiation-induced activation of NF-κB, thereby regulating the radiosensitivity of oral cancer cells. Key words: Salubrinal; NF-κB; Oral cancer cell line; Apoptosis; Radiosensitivity
{"title":"Salubrinal increases the apoptosis of oral cancer cells by inhibiting radiation-induced activation of NF-Κb","authors":"Jie Wang, Miao Zhang, Gaiyan Li, Xintong Lyu, Q. Qiao","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.007","url":null,"abstract":"Objective \u0000To explore the mechanism of the role of Salubrinal in regulating the radiation-induced apoptosis of oral cancer cells. \u0000 \u0000 \u0000Methods \u0000Radioresistant KBR cell line was constructed (4 Gy per fraction, every 7-10 d for 4 times). The radiosensitivity of oral cancer cells after Salubrinal pretreatment was measured by colony formation assay. The expression levels of NF-κB-HIF-1α signaling pathway and apoptosis biomarker cleaved PARP in oral cancer cells were measured by Western blot. The apoptosis rate was detected by Annexin V, PI staining and flow cytometry. \u0000 \u0000 \u0000Results \u0000Colony formation assay demonstrated that Salubrinal increased the radiosensitivity of oral cancer cells. The radiosensitization ratios of KB and KBR cells were 1.19 and 1.24. Western blot revealed that the activation of NF-κB-HIF-1α was time-dependent in the radiation-induced oral cancer cells, whereas Salubrinal inhibited the radiation-induced abnormal activation. In addition, Salubrinal increased the expression of apoptosis biomarker cleaved PARP and apoptosis index in radiation-induced oral cancer cells, whereas TNF-α, an activator of NF-κB, reversed the effect, suggesting that Salubrinal increased the apoptosis of radiation-induced oral cancer cells by suppressing the activation of NF-κB. Pretreatment of NF-κB inhibitor Bay11-7082 also increased the cell apoptosis. The expression levels of cleaved PARP of KB and KBR cell lines in the Bay11-7082+ IR group were 2.67±0.26 and 1.91±0.17, significantly higher compared with 2.1±0.16 and 1.44±0.15 in the IR group (both P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Salubrinal can aggravate the apoptosis of radiation-induced oral cancer cells by inhibiting the radiation-induced activation of NF-κB, thereby regulating the radiosensitivity of oral cancer cells. \u0000 \u0000 \u0000Key words: \u0000Salubrinal; NF-κB; Oral cancer cell line; Apoptosis; Radiosensitivity","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"111-114"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49228582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.016
Shanshan Zhang, Yuntian Shen, Q. Fan, Ye Tian, Qiang Huang
After multidisciplinary treatment including radiotherapy, the median survival of patients with glioblastoma multiforme (GBM) remains approximately 1 year. The heterogeneity of the genome and proteome of glioblastoma stem cells (GSC) is the fundamental factor affecting the prognosis. Proteomics-based sensitization of key radioresistance proteins is expected to improve the prognosis of GBM patients. In this article, literature review was conducted from PubMed and other databases in the previous 10 years to systematically discuss the research progress on various commonly used protein quantitative techniques, tools for data processing analysis and the application in radioresistance and radiosensitization of GSCs. Key words: Gioma stem cell; Radiation sensitivity; Proteomics
{"title":"Research progress on radiation-resistant and radiation-sensitive proteomics in glioma stem cells","authors":"Shanshan Zhang, Yuntian Shen, Q. Fan, Ye Tian, Qiang Huang","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.016","url":null,"abstract":"After multidisciplinary treatment including radiotherapy, the median survival of patients with glioblastoma multiforme (GBM) remains approximately 1 year. The heterogeneity of the genome and proteome of glioblastoma stem cells (GSC) is the fundamental factor affecting the prognosis. Proteomics-based sensitization of key radioresistance proteins is expected to improve the prognosis of GBM patients. In this article, literature review was conducted from PubMed and other databases in the previous 10 years to systematically discuss the research progress on various commonly used protein quantitative techniques, tools for data processing analysis and the application in radioresistance and radiosensitization of GSCs. \u0000 \u0000 \u0000Key words: \u0000Gioma stem cell; Radiation sensitivity; Proteomics","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"150-153"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48471610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.005
Junjun Guo, W. Gao, Qian Wang, Shenbo Fu, P. Xi
Objective To investigate the clinical significance of radiotherapy for stage Ⅳ esophageal cancer. Methods Clinical data of 133 stage Ⅳ esophageal cancer patients admitted to our hospital from 2012 to 2018 were retrospectively analyzed. All patients were assigned into the radiochemotherapy (n=89) and chemotherapy groups (n=44). The survival analysis was performed by Kaplan-Meier method. The multivariate prognostic analysis was conducted by Cox’s regression model. Results The 1-, 2-and 3-year overall survival rates of the entire cohort were 53.5%, 20.4% and13.6% respectively. Cox’s regression analysis showed that gender, ECOG score, number of distant metastases, and whether the primary lesions received radiotherapy were the independent prognostic factors (all P 50Gy and ≤50Gy was 14.3 months and 8.2 months (P<0.05), 8.6 months and 2.8 months for the PFS (P<0.05), and 15.2 months and 4.7 months for the LRFS (P<0.05), respectively. The number of distant metastases and the clinical efficacy for primary lesions were the independent prognostic factors in the radiochemotherapy group (both P<0.05). Conclusion Radiotherapy can improve the clinical prognosis of patients with stage Ⅳ esophageal cancer. Key words: Esophageal neoplasm/radiochemotherapy; Esophageal neoplasm/chemotherapy; Prognosis
{"title":"Retrospective clinical analysis of 133 cases of stage IV esophageal cancer","authors":"Junjun Guo, W. Gao, Qian Wang, Shenbo Fu, P. Xi","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.005","url":null,"abstract":"Objective \u0000To investigate the clinical significance of radiotherapy for stage Ⅳ esophageal cancer. \u0000 \u0000 \u0000Methods \u0000Clinical data of 133 stage Ⅳ esophageal cancer patients admitted to our hospital from 2012 to 2018 were retrospectively analyzed. All patients were assigned into the radiochemotherapy (n=89) and chemotherapy groups (n=44). The survival analysis was performed by Kaplan-Meier method. The multivariate prognostic analysis was conducted by Cox’s regression model. \u0000 \u0000 \u0000Results \u0000The 1-, 2-and 3-year overall survival rates of the entire cohort were 53.5%, 20.4% and13.6% respectively. Cox’s regression analysis showed that gender, ECOG score, number of distant metastases, and whether the primary lesions received radiotherapy were the independent prognostic factors (all P 50Gy and ≤50Gy was 14.3 months and 8.2 months (P<0.05), 8.6 months and 2.8 months for the PFS (P<0.05), and 15.2 months and 4.7 months for the LRFS (P<0.05), respectively. The number of distant metastases and the clinical efficacy for primary lesions were the independent prognostic factors in the radiochemotherapy group (both P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Radiotherapy can improve the clinical prognosis of patients with stage Ⅳ esophageal cancer. \u0000 \u0000 \u0000Key words: \u0000Esophageal neoplasm/radiochemotherapy; Esophageal neoplasm/chemotherapy; Prognosis","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"102-105"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49619499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.018
Jing Zeng, Q. Pang, Ping Wang, P. Qu, Shan Jiang
Brachytherapy is an integral part of radiotherapy treatment for cervical cancer. With the rapid development of medical imaging technology, three-dimensional (3D) imaging modality has been applied in the brachytherapy for cervical cancer. After computed tomography (CT) and magnetic resonance imaging (MRI), 3D ultrasound has been gradually applied to guide the brachytherapy for cervical cancer due to its superior soft tissue imaging characteristics, economy, high efficiency and convenience. In this article, the research progress on the application of ultrasonography in brachytherapy was summarized according to literature review, aiming to provide reference for subsequent research. Key words: Cervical neoplasm/brachytherapy; Ultrasound image guidance; Image registration
{"title":"Research progress on ultrasound image-guided brachytherapy for cervical cancer","authors":"Jing Zeng, Q. Pang, Ping Wang, P. Qu, Shan Jiang","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.018","url":null,"abstract":"Brachytherapy is an integral part of radiotherapy treatment for cervical cancer. With the rapid development of medical imaging technology, three-dimensional (3D) imaging modality has been applied in the brachytherapy for cervical cancer. After computed tomography (CT) and magnetic resonance imaging (MRI), 3D ultrasound has been gradually applied to guide the brachytherapy for cervical cancer due to its superior soft tissue imaging characteristics, economy, high efficiency and convenience. In this article, the research progress on the application of ultrasonography in brachytherapy was summarized according to literature review, aiming to provide reference for subsequent research. \u0000 \u0000 \u0000Key words: \u0000Cervical neoplasm/brachytherapy; Ultrasound image guidance; Image registration","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"158-160"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44902752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.010
Jian Cui, Yuliang Jiang, Z. Ji, F. Guo, R. Peng, Haitao Sun, J. Fan, Weiyan Li
Objective To compare the preoperative and postoperative dosimetric parameters in the treatment of spinal metastasis, and to verify the accuracy of 3D-printing non-coplanar template (3D-PNCT) combined with CT-guided 125I seed implantation for the treatment of spinal metastasis. Methods The treatment plans of 7 patients with spinal metastasis (9 lesions) from 2016 to 2018 receiving 3D-PNCT in combination with CT-guided 125I seed implantation were retrospectively analyzed. The dosimetric parameters including homogeneity index (HI), conformal index (CI), external index (EI), dose of 90% target volume(D90), mPD, volume percent of 100%, 150%, and 200% prescribed dose V100、V150、V200 and D2cm3 of spinal cord were compared before and after operation. The british columbia cancer ageny particle implantation quality evaluation standard was applied to evaluate the quality of implantation. Results The HI, EI and CI, D90, mPD, V100, V150, V200 and D2cm3 of spinal cord did not significantly differ before and after the plan (all P>0.05). Five were evaluated as excellent and 4 were assessed as good. Conclusion The postoperative dosimetric parameters of 3D-PNCT combined with CT guided 125I seed implantation of spinal metastasis are basically consistent with preoperative dosimetric parameters. The postoperative plans are evaluated as excellent or good, suggesting that the technology has a good therapeutic accuracy in the treatment of spinal metastasis. Key words: 3D-printing non-coplanar template; Seed implantation; Spinal metastasis; Dosimetry
{"title":"Dosimetric analysis of 3D-printing non-coplanar template combined with CT-guided125I seed implantation for the treatment of spinal metastasis","authors":"Jian Cui, Yuliang Jiang, Z. Ji, F. Guo, R. Peng, Haitao Sun, J. Fan, Weiyan Li","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.010","url":null,"abstract":"Objective \u0000To compare the preoperative and postoperative dosimetric parameters in the treatment of spinal metastasis, and to verify the accuracy of 3D-printing non-coplanar template (3D-PNCT) combined with CT-guided 125I seed implantation for the treatment of spinal metastasis. \u0000 \u0000 \u0000Methods \u0000The treatment plans of 7 patients with spinal metastasis (9 lesions) from 2016 to 2018 receiving 3D-PNCT in combination with CT-guided 125I seed implantation were retrospectively analyzed. The dosimetric parameters including homogeneity index (HI), conformal index (CI), external index (EI), dose of 90% target volume(D90), mPD, volume percent of 100%, 150%, and 200% prescribed dose V100、V150、V200 and D2cm3 of spinal cord were compared before and after operation. The british columbia cancer ageny particle implantation quality evaluation standard was applied to evaluate the quality of implantation. \u0000 \u0000 \u0000Results \u0000The HI, EI and CI, D90, mPD, V100, V150, V200 and D2cm3 of spinal cord did not significantly differ before and after the plan (all P>0.05). Five were evaluated as excellent and 4 were assessed as good. \u0000 \u0000 \u0000Conclusion \u0000The postoperative dosimetric parameters of 3D-PNCT combined with CT guided 125I seed implantation of spinal metastasis are basically consistent with preoperative dosimetric parameters. The postoperative plans are evaluated as excellent or good, suggesting that the technology has a good therapeutic accuracy in the treatment of spinal metastasis. \u0000 \u0000 \u0000Key words: \u00003D-printing non-coplanar template; Seed implantation; Spinal metastasis; Dosimetry","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"122-125"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47039411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.009
Hui Zhang, Hong-Yan Liang
Objection To investigate the effect of miR-32-5p on the radiosensitivity, migration and invasion of colorectal cancer cells and the underlying mechanism. Methods Human colorectal cancer SW480 cells and normal colonic epithelial NCM460 cells were cultured. The colorectal cancer cells were divided into the non-transfected and transfected groups (transfected with anti-miR-NC, anti-miR-32-5p, pcDNA, pcDNA-TOB1, anti-miR-32-5p+ si-NC and anti-miR-32-5p+ si-TOB1, respectively). The expression of miR-32-5p and TOB1 at the mRNA and protein levels was detected by RT-qPCR and Western blot. The radiosensitivity of the transfected cells was determined by colony formation assay. The migration and invasion ability of the transfected cells were detected by Transwell assay. Whether miR-32-5p targeted TOB1 was validated by dual luciferase reporter gene assay and Western blot. Results Compared with human colonic epithelial cells, the expression of miR-32-5p was significantly up-regulated, whereas the expression of TOB1 mRNA and protein was remarkably down-regulated in the colon cancer cells (all P<0.05). Compared with the anti-miR-NC, the quantity of cell migration and invasion was significantly decreased (both P<0.05) and the radiosensitivity ratio was 1.801 in the anti-miR-32-5p group. Compared with the pcDNA group, the quantity of cell migration and invasion was significantly decreased (both P<0.05) and the radiosensitivity ratio was 1.764 in the pcDNA-TOB1 group. Dual luciferase reporter gene assay and Western blot confirmed that miR-32-5p negatively regulated the expression of TOB1 protein. Compared with the anti-miR-32-5p+ si-NC group, the quantity of cell migration and invasion was significantly increased (both P<0.05) and the radiosensitivity ratio was 0.591 in the anti-miR-32-5p+ si-TOB1 group. Conclusions Inhibition of miR-32-5p expression can significantly enhance the radiosensitivity of colorectal cancer cells and suppress cell migration and invasion. The underlying mechanism might be related to the targeted up-regulation of TOB1 expression. Key words: Colorectal cancer cell line; miR-32-5p gene; TOB1 gene; Radiosensitivity; Migration and invasion
{"title":"Mechanism of miR-32-5p targeting TOB1 gene in regulating radiosensitization, migration and invasion of colorectal cancer cells","authors":"Hui Zhang, Hong-Yan Liang","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.009","url":null,"abstract":"Objection \u0000To investigate the effect of miR-32-5p on the radiosensitivity, migration and invasion of colorectal cancer cells and the underlying mechanism. \u0000 \u0000 \u0000Methods \u0000Human colorectal cancer SW480 cells and normal colonic epithelial NCM460 cells were cultured. The colorectal cancer cells were divided into the non-transfected and transfected groups (transfected with anti-miR-NC, anti-miR-32-5p, pcDNA, pcDNA-TOB1, anti-miR-32-5p+ si-NC and anti-miR-32-5p+ si-TOB1, respectively). The expression of miR-32-5p and TOB1 at the mRNA and protein levels was detected by RT-qPCR and Western blot. The radiosensitivity of the transfected cells was determined by colony formation assay. The migration and invasion ability of the transfected cells were detected by Transwell assay. Whether miR-32-5p targeted TOB1 was validated by dual luciferase reporter gene assay and Western blot. \u0000 \u0000 \u0000Results \u0000Compared with human colonic epithelial cells, the expression of miR-32-5p was significantly up-regulated, whereas the expression of TOB1 mRNA and protein was remarkably down-regulated in the colon cancer cells (all P<0.05). Compared with the anti-miR-NC, the quantity of cell migration and invasion was significantly decreased (both P<0.05) and the radiosensitivity ratio was 1.801 in the anti-miR-32-5p group. Compared with the pcDNA group, the quantity of cell migration and invasion was significantly decreased (both P<0.05) and the radiosensitivity ratio was 1.764 in the pcDNA-TOB1 group. Dual luciferase reporter gene assay and Western blot confirmed that miR-32-5p negatively regulated the expression of TOB1 protein. Compared with the anti-miR-32-5p+ si-NC group, the quantity of cell migration and invasion was significantly increased (both P<0.05) and the radiosensitivity ratio was 0.591 in the anti-miR-32-5p+ si-TOB1 group. \u0000 \u0000 \u0000Conclusions \u0000Inhibition of miR-32-5p expression can significantly enhance the radiosensitivity of colorectal cancer cells and suppress cell migration and invasion. The underlying mechanism might be related to the targeted up-regulation of TOB1 expression. \u0000 \u0000 \u0000Key words: \u0000Colorectal cancer cell line; miR-32-5p gene; TOB1 gene; Radiosensitivity; Migration and invasion","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"118-121"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44264229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.003
P. Xing, Hangzhou Wang, Min Chen, Ye Tian
Objective To analyze the clinical characteristics of children with low grade glioma (LGG) and evaluate their survival status and quality of life in LGG pediatric patients after postoperative radiotherapy. Methods Clinical data of 27 LGG children aged≤14 years admitted to Department of Radiation Oncology of our hospital from January 2011 to December 2017 were retrospectively analyzed. The 5-year overall survival (OS) and progression-free survival (PFS) were estimated with the Kaplan-Meier method.The quality of life was evaluated by body mass index (BMI), WeeFIM and enrollment situation. Results The 5-year OS and PFS of 27 pediatric patients were 92% and 87%. BMI was measured in 25 children who were still alive and the results showed that 1 child was slightly emaciated, 3 were overweight and 21 were normal. The total score of WeeFIM was> 108 in 21 cases and< 90 in 4 cases. In terms of social role-playing assessment, 5 cases delayed enrollment for≥ 2 years, 2 cases had good adaptation except the difficulties due to lameness, 7 of them had poor study performance, but had no communication barrier. Conclusions Children with LGG can obtain relatively excellent OS and PFS after postoperative radiotherapy. Part of the children with long-term survival may experience a declined quality of life, which is mainly manifested with a decline in motor and cognitive functions. Key words: Pediatric low grade glioma/radiotherapy; Survival; Quality of life
{"title":"Survival analysis and quality of life of pediatric low grade glioma treated by postoperative radiotherapy","authors":"P. Xing, Hangzhou Wang, Min Chen, Ye Tian","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.003","url":null,"abstract":"Objective \u0000To analyze the clinical characteristics of children with low grade glioma (LGG) and evaluate their survival status and quality of life in LGG pediatric patients after postoperative radiotherapy. \u0000 \u0000 \u0000Methods \u0000Clinical data of 27 LGG children aged≤14 years admitted to Department of Radiation Oncology of our hospital from January 2011 to December 2017 were retrospectively analyzed. The 5-year overall survival (OS) and progression-free survival (PFS) were estimated with the Kaplan-Meier method.The quality of life was evaluated by body mass index (BMI), WeeFIM and enrollment situation. \u0000 \u0000 \u0000Results \u0000The 5-year OS and PFS of 27 pediatric patients were 92% and 87%. BMI was measured in 25 children who were still alive and the results showed that 1 child was slightly emaciated, 3 were overweight and 21 were normal. The total score of WeeFIM was> 108 in 21 cases and< 90 in 4 cases. In terms of social role-playing assessment, 5 cases delayed enrollment for≥ 2 years, 2 cases had good adaptation except the difficulties due to lameness, 7 of them had poor study performance, but had no communication barrier. \u0000 \u0000 \u0000Conclusions \u0000Children with LGG can obtain relatively excellent OS and PFS after postoperative radiotherapy. Part of the children with long-term survival may experience a declined quality of life, which is mainly manifested with a decline in motor and cognitive functions. \u0000 \u0000 \u0000Key words: \u0000Pediatric low grade glioma/radiotherapy; Survival; Quality of life","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"93-95"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41362943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-15DOI: 10.3760/CMA.J.ISSN.1004-4221.2020.02.006
X. Bai, Binbing Wang, K. Shao, Yiwei Yang, G. Shan
Objective To study a lung dose prediction method for the early stage non-small cell lung cancer (NSCLC) treated with stereotactic body radiotherapy based on machine learning algorithm, and to evaluate the feasibility of application in planning quality assurance. Methods A machine learning algorithm was utilized to achieve DVH prediction. First, an expert plan dataset with 125 cases was built, and the geometric features of ROI, beam angle and dose-volume histogram(DVH) parameters in the dataset were extracted. Following a correlation model was established between the features and DVHs. Second, the geometric and beam features from 10 cases outside the training pool were extracted, and the model was adopted to predict the achievable DVHs values of the lung. The predicted DVHs values were compared with the actual planned results. Results The mean squared errors of external validation for the 10 cases in mean lung dose (MLD)MLD and V20 of the lung were 91.95 cGy and 3.12%, respectively. Two cases whose lung doses were higher than the predicted values were re-planned, and the results showed that the the lung doses were reduced. Conclusion It is feasible to utilize the anatomy and beam angle features to predict the lung DVH parameters for plan evaluation and quality assurance in early stage NSCLC patients treated with stereotactic body radiotherapy Key words: Lung neoplasm/stereotactic body radiotherapy; Machine learning; Dose volume histogram
{"title":"A study of prediction model of lung dose in early stage non-small cell lung cancer with stereotactic body radiotherapy","authors":"X. Bai, Binbing Wang, K. Shao, Yiwei Yang, G. Shan","doi":"10.3760/CMA.J.ISSN.1004-4221.2020.02.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1004-4221.2020.02.006","url":null,"abstract":"Objective \u0000To study a lung dose prediction method for the early stage non-small cell lung cancer (NSCLC) treated with stereotactic body radiotherapy based on machine learning algorithm, and to evaluate the feasibility of application in planning quality assurance. \u0000 \u0000 \u0000Methods \u0000A machine learning algorithm was utilized to achieve DVH prediction. First, an expert plan dataset with 125 cases was built, and the geometric features of ROI, beam angle and dose-volume histogram(DVH) parameters in the dataset were extracted. Following a correlation model was established between the features and DVHs. Second, the geometric and beam features from 10 cases outside the training pool were extracted, and the model was adopted to predict the achievable DVHs values of the lung. The predicted DVHs values were compared with the actual planned results. \u0000 \u0000 \u0000Results \u0000The mean squared errors of external validation for the 10 cases in mean lung dose (MLD)MLD and V20 of the lung were 91.95 cGy and 3.12%, respectively. Two cases whose lung doses were higher than the predicted values were re-planned, and the results showed that the the lung doses were reduced. \u0000 \u0000 \u0000Conclusion \u0000It is feasible to utilize the anatomy and beam angle features to predict the lung DVH parameters for plan evaluation and quality assurance in early stage NSCLC patients treated with stereotactic body radiotherapy \u0000 \u0000 \u0000Key words: \u0000Lung neoplasm/stereotactic body radiotherapy; Machine learning; Dose volume histogram","PeriodicalId":10288,"journal":{"name":"Chinese Journal of Radiation Oncology","volume":"29 1","pages":"106-110"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44037703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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