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Collection of Czechoslovak Chemical Communications最新文献

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Generation of high affinity DNA aptamers by the expansion of the genetic alphabet 通过基因字母表的扩展产生高亲和力的DNA适体
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414097
M. Kimoto, Ken-ichiro Matsunaga, I. Hirao
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引用次数: 0
DNA polymerases in action with modified substrates DNA聚合酶在修饰底物中的作用
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414101
K. Bergen, H. Busskamp, A. Steck, S. Obeid, K. Betz, A. Marx
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引用次数: 0
Postsynthetic labeling of DNA and RNA by fluorophores 荧光团合成后标记DNA和RNA
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414190
H. Wagenknecht
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引用次数: 0
Synthesis of a DNA promoter segment containing all four epigenetic nucleosides: 5-methyl-, 5-hydroxymethyl-, 5-formyl-, and 5-carboxy-2’-deoxycytidine 包含所有四种表观遗传核苷的DNA启动子片段的合成:5-甲基,5-羟甲基,5-甲酰基和5-羧基-2 ' -脱氧胞苷
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414363
A. Schröder, J. Steinbacher, B. Steigenberger, Felix A Gnerlich, Stefan Schiesser, T. Pfaffeneder, T. Carell
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引用次数: 2
Formation of DNA G-quadruplex through long-lived intermediates 通过长寿命中间体形成DNA g-四重体
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414367
P. Šket, S. Čeru, I. Prislan, J. Lah, J. Plavec
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引用次数: 0
The loss of TEAH + cation from certain nucleotide analogues 某些核苷酸类似物中TEAH +阳离子的丢失
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414373
K. Kolodziej, A. Kraszewski, J. Stawinski, M. Sobkowski
Phosphorus esters of biomolecules are often isolated in a form of triethylammonium salts, which are usually stable, amorphous solids of good solubility both in water and organic solvents, and are obtained directly after chromatography performed in the presence of triethylamine. However, during our recent studies on nucleoside phosphoramidates of type 1 we consistently isolated them as free acids instead of the expected TEAH+ salts (Fig. 1).1 Th is may aff ect the stability and solubility of a product, lead to acidifi cation of its solutions and cause false calculations due to decrease of the molecular mass. Th e lability of the TEAH+ cation in these compounds was assigned to an equilibrium in which free TEA was released to the solution and evacuated during concentration, leaving behind the nucleotidic counterpart that may exist in several putative forms 1A.1 Some further observations regarding this phenomenon are outlined in this communication.
生物分子的磷酯通常以三乙胺盐的形式分离,三乙胺盐通常是稳定的无定形固体,在水和有机溶剂中都具有良好的溶解度,并且在三乙胺存在下进行色谱后直接获得。然而,在我们最近对1型核苷磷酸酯的研究中,我们始终将它们作为游离酸而不是预期的TEAH+盐分离出来(图1)这可能会影响产品的稳定性和溶解度,导致其溶液酸化,并由于分子质量的减少而导致错误的计算。这些化合物中TEAH+阳离子的稳定性被分配到一种平衡状态,在这种平衡状态下,游离的TEA被释放到溶液中,并在浓缩过程中被抽离,留下可能以几种假定形式存在的核苷对应物a1本报告概述了关于这一现象的一些进一步观察。
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引用次数: 0
Artificial synthesis of histone H2A and its application for live-cell imaging 组蛋白H2A的人工合成及其在活细胞成像中的应用
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414377
Takuma Sueoka, Gosuke Hayashi, A. Okamoto
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引用次数: 0
Evaluation of biological activity of trimethylguanosine cap analogs modified within the 5’,5’-triphosphate bridge 5 ',5 ' -三磷酸桥修饰的三甲基鸟苷帽类似物的生物活性评价
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414416
Małgorzata Żytek, J. Kowalska, Maciej Lukaszewicz, E. Darżynkiewicz, A. Niedźwiecka, J. Jemielity
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引用次数: 0
Reversible DNA and RNA methylation in biological regulation 生物调控中的可逆DNA和RNA甲基化
Pub Date : 2014-01-01 DOI: 10.1135/css201414052
Chuan He
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引用次数: 0
Touching base with arginine: recognition of O 6 -alkyl guanines by alkyltransferase-like protein Atl1 与精氨酸接触碱:烷基转移酶样蛋白Atl1识别O 6 -烷基鸟嘌呤
Pub Date : 2014-01-01 DOI: 10.1135/CSS201414147
O. Wilkinson, V. Latypov, J. L. Tubbs, Christopher L. Millington, R. Morita, H. Blackburn, A. Marriott, G. McGown, M. Thorncroft, A. Watson, B. Connolly, J. Grasby, R. Masui, C. Hunter, J. Tainer, G. Margison, David M Williams
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引用次数: 0
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Collection of Czechoslovak Chemical Communications
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