{"title":"Generation of high affinity DNA aptamers by the expansion of the genetic alphabet","authors":"M. Kimoto, Ken-ichiro Matsunaga, I. Hirao","doi":"10.1135/CSS201414097","DOIUrl":"https://doi.org/10.1135/CSS201414097","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"97-100"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Bergen, H. Busskamp, A. Steck, S. Obeid, K. Betz, A. Marx
{"title":"DNA polymerases in action with modified substrates","authors":"K. Bergen, H. Busskamp, A. Steck, S. Obeid, K. Betz, A. Marx","doi":"10.1135/CSS201414101","DOIUrl":"https://doi.org/10.1135/CSS201414101","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"101-102"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Postsynthetic labeling of DNA and RNA by fluorophores","authors":"H. Wagenknecht","doi":"10.1135/CSS201414190","DOIUrl":"https://doi.org/10.1135/CSS201414190","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"190-192"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Schröder, J. Steinbacher, B. Steigenberger, Felix A Gnerlich, Stefan Schiesser, T. Pfaffeneder, T. Carell
{"title":"Synthesis of a DNA promoter segment containing all four epigenetic nucleosides: 5-methyl-, 5-hydroxymethyl-, 5-formyl-, and 5-carboxy-2’-deoxycytidine","authors":"A. Schröder, J. Steinbacher, B. Steigenberger, Felix A Gnerlich, Stefan Schiesser, T. Pfaffeneder, T. Carell","doi":"10.1135/CSS201414363","DOIUrl":"https://doi.org/10.1135/CSS201414363","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"363-364"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Formation of DNA G-quadruplex through long-lived intermediates","authors":"P. Šket, S. Čeru, I. Prislan, J. Lah, J. Plavec","doi":"10.1135/CSS201414367","DOIUrl":"https://doi.org/10.1135/CSS201414367","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"367-368"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kolodziej, A. Kraszewski, J. Stawinski, M. Sobkowski
Phosphorus esters of biomolecules are often isolated in a form of triethylammonium salts, which are usually stable, amorphous solids of good solubility both in water and organic solvents, and are obtained directly after chromatography performed in the presence of triethylamine. However, during our recent studies on nucleoside phosphoramidates of type 1 we consistently isolated them as free acids instead of the expected TEAH+ salts (Fig. 1).1 Th is may aff ect the stability and solubility of a product, lead to acidifi cation of its solutions and cause false calculations due to decrease of the molecular mass. Th e lability of the TEAH+ cation in these compounds was assigned to an equilibrium in which free TEA was released to the solution and evacuated during concentration, leaving behind the nucleotidic counterpart that may exist in several putative forms 1A.1 Some further observations regarding this phenomenon are outlined in this communication.
{"title":"The loss of TEAH + cation from certain nucleotide analogues","authors":"K. Kolodziej, A. Kraszewski, J. Stawinski, M. Sobkowski","doi":"10.1135/CSS201414373","DOIUrl":"https://doi.org/10.1135/CSS201414373","url":null,"abstract":"Phosphorus esters of biomolecules are often isolated in a form of triethylammonium salts, which are usually stable, amorphous solids of good solubility both in water and organic solvents, and are obtained directly after chromatography performed in the presence of triethylamine. However, during our recent studies on nucleoside phosphoramidates of type 1 we consistently isolated them as free acids instead of the expected TEAH+ salts (Fig. 1).1 Th is may aff ect the stability and solubility of a product, lead to acidifi cation of its solutions and cause false calculations due to decrease of the molecular mass. Th e lability of the TEAH+ cation in these compounds was assigned to an equilibrium in which free TEA was released to the solution and evacuated during concentration, leaving behind the nucleotidic counterpart that may exist in several putative forms 1A.1 Some further observations regarding this phenomenon are outlined in this communication.","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"373-374"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Artificial synthesis of histone H2A and its application for live-cell imaging","authors":"Takuma Sueoka, Gosuke Hayashi, A. Okamoto","doi":"10.1135/CSS201414377","DOIUrl":"https://doi.org/10.1135/CSS201414377","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"377-378"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Małgorzata Żytek, J. Kowalska, Maciej Lukaszewicz, E. Darżynkiewicz, A. Niedźwiecka, J. Jemielity
{"title":"Evaluation of biological activity of trimethylguanosine cap analogs modified within the 5’,5’-triphosphate bridge","authors":"Małgorzata Żytek, J. Kowalska, Maciej Lukaszewicz, E. Darżynkiewicz, A. Niedźwiecka, J. Jemielity","doi":"10.1135/CSS201414416","DOIUrl":"https://doi.org/10.1135/CSS201414416","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"416-417"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63713965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reversible DNA and RNA methylation in biological regulation","authors":"Chuan He","doi":"10.1135/css201414052","DOIUrl":"https://doi.org/10.1135/css201414052","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"52-55"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63712434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Wilkinson, V. Latypov, J. L. Tubbs, Christopher L. Millington, R. Morita, H. Blackburn, A. Marriott, G. McGown, M. Thorncroft, A. Watson, B. Connolly, J. Grasby, R. Masui, C. Hunter, J. Tainer, G. Margison, David M Williams
{"title":"Touching base with arginine: recognition of O 6 -alkyl guanines by alkyltransferase-like protein Atl1","authors":"O. Wilkinson, V. Latypov, J. L. Tubbs, Christopher L. Millington, R. Morita, H. Blackburn, A. Marriott, G. McGown, M. Thorncroft, A. Watson, B. Connolly, J. Grasby, R. Masui, C. Hunter, J. Tainer, G. Margison, David M Williams","doi":"10.1135/CSS201414147","DOIUrl":"https://doi.org/10.1135/CSS201414147","url":null,"abstract":"","PeriodicalId":10674,"journal":{"name":"Collection of Czechoslovak Chemical Communications","volume":"14 1","pages":"147-150"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63712834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}