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Characterization of the JAR Sealants in the Fluid Collections of the Muséum National D'histoire Naturelle
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.87
M. Sablier, C. Davron, M. Herbin
Over the centuries, the composition of jar sealants in natural history museum fluid collections has changed but has not been the subject of extensive documentation. Consequently, it is of paramount interest to increase our knowledge of sealant composition as well as to correlate this information with the date of manufacture and type of preparation. As an initial step towards achieving this goal, an analytical protocol was developed to characterize the composition of sealants encountered in fluid collections of the Muséum national d'Histoire naturelle (MNHN) in Paris. Fourier transform-infrared (FT-IR) spectroscopy and pyrolysis-gas chromatography/mass spectrometry (GC/MS) were used to investigate the nature of selected jar sealants, and these techniques proved to be efficient for the identification and characterization of waxes and resin additives in sealing samples. First results are very promising; similar examinations should be continued on a large scale for an exhaustive analysis of the MNHN fluid collections.
几个世纪以来,自然历史博物馆液体藏品中罐子密封剂的成分发生了变化,但并没有广泛的文献记录。因此,增加我们对密封剂成分的知识以及将这些信息与生产日期和制剂类型联系起来是至关重要的。作为实现这一目标的第一步,制定了一项分析议定书,以确定巴黎国家自然历史博物馆(MNHN)液体收集中遇到的密封剂的成分。采用傅里叶变换-红外光谱(FT-IR)和热解-气相色谱/质谱(GC/MS)对所选罐密封胶的性质进行了研究,结果表明,这些技术对密封样品中蜡和树脂添加剂的鉴定和表征是有效的。最初的结果非常有希望;应继续进行大规模的类似检查,以便对MNHN流体收集进行详尽的分析。
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引用次数: 1
Preservation of Natural History Wet Collections: Feedback and Prospects: Proceedings of a Conference at the French Natural History Museum, 5–7 December 2018 自然历史湿藏品的保存:反馈和前景:法国自然历史博物馆会议记录,2018年12月5-7日
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.1
S. Cersoy, John Simmons, J. Carter, D. Neumann, M. Herbin, V. Rouchon
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引用次数: 0
Salting Out: A Simple and Reliable Method to Distinguish Between Common Fluid Preservatives and Estimate Alcohol Concentration 盐析:一种简单可靠的液体防腐剂鉴别及酒精浓度测定方法
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.11
I. Finkelde, G., Asher Newsome
This paper details the salting-out method, which uses the salts potassium carbonate and sodium chloride to distinguish between the three most commonly used fluid preservatives: ethanol, isopropanol, and formalin. A summary of other methods to identify fluid preservative type and a review of the salting-out method published by Mayfield (2013, Distinguishing between ethanol and isopropanol in natural history collection fluid storage, Society for the Preservation of Natural History Collections, https://spnhc.org/wp-content/uploads/2018/11/Mayfieldfinalwithtablechanges.pdf) are provided. A new salting-out method is presented, which requires a small fluid sample (2–4 ml). It is simple, quick, and relatively inexpensive to implement, making it a viable method to distinguish between common fluid preservatives. The materials and equipment for the salting-out test cost just over $100 US, and tests take approximately 3 minutes per container. Results of testing on known concentrations and combinations of ethanol, isopropanol, and formalin (a solution of formaldehyde in water) and on samples of fluid preservatives from specimen containers in the Smithsonian National Museum of Natural History and Bernice Pauahi Bishop Museum collections are presented. The results of salting-out tests have been verified by direct analysis in real time mass spectrometry (DART-MS) (Cody et al., 2005, Versatile new ion source for the analysis of materials in open air under ambient conditions, Analytical Chemistry 77(8):2297–302), which confirmed the results of salting-out tests but also highlighted some limitations, particularly when combinations of fluid preservative are encountered.
本文详细介绍了盐析方法,该方法使用碳酸钾和氯化钠盐来区分三种最常用的液体防腐剂:乙醇、异丙醇和福尔马林。本文总结了鉴定液体防腐剂类型的其他方法,并对Mayfield发表的盐析方法进行了回顾(2013年,《区分自然历史藏品液体储存中的乙醇和异丙醇》,自然历史藏品保存协会,https://spnhc.org/wp-content/uploads/2018/11/Mayfieldfinalwithtablechanges.pdf)。提出了一种新的盐析方法,该方法需要少量的液体样品(2-4 ml)。该方法简单、快速且相对便宜,是区分常见液体防腐剂的可行方法。盐析测试的材料和设备成本仅为100多美元,每个容器的测试时间约为3分钟。介绍了对已知浓度和乙醇、异丙醇和福尔马林(甲醛在水中的溶液)组合的测试结果,以及对史密森国家自然历史博物馆和伯尼斯·波阿希·毕肖普博物馆收藏的标本容器中的液体防腐剂样品的测试结果。盐析试验的结果已通过实时质谱直接分析(DART-MS)得到验证(Cody等人,2005年,用于在露天环境条件下分析材料的多功能新离子源,分析化学77(8):2297-302),这证实了盐析试验的结果,但也强调了一些局限性,特别是在遇到液体防腐剂组合时。
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引用次数: 0
Conservation-Restoration of a Botanical Museum Fluid Collection: Practice and Research 植物博物馆流体藏品的保护与修复:实践与研究
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.143
M. Dangeon, E. Cornet, L. Brambilla
The collection of the Botanical Museum of the University of Zürich is an academic collection assembled from 1891 to the end of the 20th century (1992 for the last inventoried item). Preserved plants come from all over the world (40 countries) and include all categories of existing Plantae (algae, lichens, fungi, higher plants, bacteriae). The fluid collection, largely neglected since 1976, shows significant degradation. The main problem is loss of preservative fluid due to leakage of the jars and aging of the seals. Another issue is the discoloration of the specimen fluids. These issues led to a research project titled FLUIDIS, which aimed to explore different preservative solutions and their impact on the discoloration of plant specimens. Conservation-restoration work was carried out on the jars of the “Professor Ernst Collection.” Topping up of was necessary for the entire collection. Restoration was performed after opening the containers and identifying the fluid. The specimens were consolidated, repaired, and mounted when necessary, then gradually put back into alcoholic solutions and finally sealed. An overall intervention protocol was established for the treatment of the entire botanical fluid collection. Its application, however, requires a careful study of each specimen.
zrich大学植物博物馆的藏品是从1891年到20世纪末收集的学术藏品(最后一次库存项目是1992年)。保存下来的植物来自世界各地(40个国家),包括现有植物的所有类别(藻类、地衣、真菌、高等植物、细菌)。自1976年以来基本上被忽视的流体收集显示出明显的退化。主要的问题是由于罐子的泄漏和密封的老化而造成的防腐剂液体的损失。另一个问题是样品液体的变色。这些问题导致了一个名为FLUIDIS的研究项目,旨在探索不同的防腐剂解决方案及其对植物标本变色的影响。对“恩斯特教授收藏”的坛子进行了保护修复工作。对整个系列都有必要进行充值。在打开容器并识别液体后进行修复。标本固结、修复,必要时安装,然后逐渐放回酒精溶液中,最后密封。建立了一个整体干预方案,用于处理整个植物液体收集。然而,它的应用需要仔细研究每个标本。
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引用次数: 0
Comparing Methods of Determining Formalin Concentration in Fluid Preservatives 液体防腐剂中福尔马林浓度测定方法的比较
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.32
I. Finkelde, R. Waller
Formalin (3.7% weight/weight [w/w], 4% weight/volume [w/v] aqueous formaldehyde) is commonly used as a fixative to prevent postmortem changes in the tissues of a specimen. The specimen is then either maintained in formalin or transferred to another fluid preservative, such as ethanol or isopropanol, for long-term preservation. Residual formalin often remains in the preservation fluid. As formaldehyde is a known carcinogen, it is important to know the concentration present in preservation fluids. A titration method has been developed to determine formalin concentration in fluid preservatives. It utilizes sodium sulfite to react with formaldehyde in solution. An adjustable, repeating pipette and a digital titrator allow for the rapid determination of formalin concentrations in small samples (<1 ml). This method of titration is compared with three commercially available methods of determining formaldehyde and formalin concentration: two brands of formaldehyde test strips, Quantofix® (Machery-Nagel GmbH) and MQuant® (EMD Millipore Corp), and a drop count titration test kit (Hach® formaldehyde test kit, model FM-1). A comparison and evaluation are made on the effectiveness of each method in determining the concentration of formalin in preservation fluids.
福尔马林(3.7%重量/重量[w/w], 4%重量/体积[w/v]的含水甲醛)通常用作固定剂,以防止死后标本组织的变化。然后将标本保存在福尔马林中或转移到另一种液体防腐剂中,如乙醇或异丙醇,以便长期保存。残留的福尔马林常残留在保存液中。由于甲醛是一种已知的致癌物,因此了解保存液中甲醛的浓度非常重要。建立了一种测定液体防腐剂中福尔马林浓度的滴定法。它利用亚硫酸钠与溶液中的甲醛发生反应。可调节的重复移液器和数字滴定器可快速测定小样品(< 1ml)中的福尔马林浓度。该方法与三种市售的测定甲醛和福尔马林浓度的方法进行了比较:两种品牌的甲醛试纸,Quantofix®(Machery-Nagel GmbH)和MQuant®(EMD Millipore Corp),以及滴计数滴定测试试剂盒(Hach®甲醛测试试剂盒,型号为dm -1)。对各种方法测定保存液中福尔马林浓度的有效性进行了比较和评价。
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引用次数: 2
A Spirit Collection of Vascular Plants at the Muséum National D'histoire Naturelle Herbarium musum国家自然历史植物标本室维管植物的精神收藏
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.130
T. Deroin, S. Lacoste
During the completion of the 2006–2013 renovation project of the Muséum National d’Histoire Naturelle herbarium in Paris, all fluid-preserved specimens were transferred to three storage areas on the fourth floor of the botany building in the Jardin des Plantes. This fluid-preserved spirit collection includes ca. 15,000 jars containing vascular plants, mainly angiosperms collected since 1900, many of which are linked to additional specimens and preparations (herbarium sheets, wood samples, and DNA samples) stored elsewhere, such as in the actual herbarium. These fluid-preserved specimens are essential for thoroughly studying and illustrating succulents and pulpy fruits as well as for the floral morphology of some families (e.g., Orchidaceae or Zingiberaceae) and are thus often sampled and sometimes further processed by visiting specialists. They are also primary sources for other collections, such as the anatomical microslide library (“Histothèque”) for examining cytological details and the pollen slide library (“Palynothèque”). Maintenance and safety of the fluid collections were greatly improved by the addition of an extractor hood that made the practical work of fixation, preservation, rehydration, bottling, and rebottling of specimens easier. The use of effective fluids, such as the fixative FAA (formaldehyde 2%, acetic acid 5%, ethanol 67%, methanol 0.4%, water 25.6%) and the glycerol/ethanol/water mixture, along with standardized glass jars, greatly improved the storage and preservation of the specimens. While it is difficult to dispense with formaldehyde due to its good fixative properties (Buesa 2008) and its accordance with classical histological methods, it needs to be removed for health and safety reasons (e.g., it is carcinogenic and affects the lungs; see Goris et al. 1998) and to avoid long-term consequences, such as decalcification or pollen exine deterioration in preserved material. The use of any alternative to formaldehyde as a preservative fluid (Carter 2012) is a priority in collection areas such as algae but not necessarily for vascular plants and mushrooms. Histological studies may be carried out on dry samples after careful rehydration with dilute ammonia (5–10% aq.) or after heating at 60°C in heavily dehydrated tissues (Espinosa and Pinedo Castro 2018), recovering their original volume and outline but usually with clearing due to loss of tannins (Deroin 1994). Retrieval of preserved material in a continuously ventilated room (70 m3) is made easier by using three kinds of arrangements: unsorted field collections in stacking tanks, easily ordered arrays in chemical safety cupboards, and reference collections on shelves, free or in glass cupboards or drawers. The reference collections fulfill different aims. Some of the specimens in this collection are reserve vouchers for phytogeographical and ethnographic exploration, such as Léon Diguet’s samples of the useful Cactaceae fromMexico gathered in 1902–1904 (Fig. 1A–C). A book
在巴黎国家自然历史博物馆2006-2013年整修项目完成期间,所有液体保存的标本都被转移到植物花园植物学大楼四楼的三个储存区。这个液体保存的标本收藏包括大约15000个装有维管植物的罐子,主要是1900年以来收集的被子植物,其中许多与储存在其他地方的其他标本和制剂(植物标本室片、木材样本和DNA样本)有关,例如在实际的植物标本室中。这些液体保存的标本对于彻底研究和说明多肉植物和果肉水果以及某些科(例如兰科或姜科)的花形态至关重要,因此经常被取样,有时由来访的专家进一步处理。它们也是其他收藏的主要来源,例如用于检查细胞学细节的解剖微玻片库(“histoth”)和花粉玻片库(“palynoth”)。液体收集的维护和安全得到了极大的改善,增加了提取罩,使标本的固定、保存、补水、装瓶和再装瓶的实际工作更容易。使用有效的液体,如固定液FAA(甲醛2%,乙酸5%,乙醇67%,甲醇0.4%,水25.6%)和甘油/乙醇/水混合物,以及标准化的玻璃罐,大大提高了标本的储存和保存。由于甲醛具有良好的固定性能(Buesa 2008),并且符合经典的组织学方法,因此很难去除甲醛,但出于健康和安全原因(例如,甲醛具有致癌性并影响肺部;(见Goris et al. 1998),并避免长期后果,如保存材料的脱钙或花粉外膜变质。使用甲醛替代品作为防腐剂(Carter 2012)在藻类等收集区域是优先考虑的,但对维管植物和蘑菇则不一定。在用稀氨(5-10% aq)仔细补液或在严重脱水的组织中60°C加热后,可以对干燥的样品进行组织学研究(Espinosa和Pinedo Castro 2018),恢复其原始体积和轮廓,但通常由于单宁的损失而进行清理(Deroin 1994)。在连续通风的房间(70立方米)中,通过使用三种安排,可以更容易地检索保存的材料:在堆垛罐中未分类的现场收集,在化学安全橱柜中易于排序的阵列,以及在架子上,免费或在玻璃橱柜或抽屉中收集参考资料。参考资料集实现了不同的目的。这个收藏中的一些标本是植物地理学和人种学探索的储备证书,例如l·迪格特1902-1904年在墨西哥采集的有用的仙人掌科标本(图1A-C)。死后出版了一本书(Diguet 1928),提供了大量详细的评论,增强了这个参考集合,这是最有可能在临时使用
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引用次数: 0
Feedback on Preservation in Fluids: the Experience of the Marine Invertebrate Collection at the Muséum National d'Histoire Naturelle 关于液体保存的反馈:国家自然历史博物馆海洋无脊椎动物收藏的经验
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.139
P.A. Martin-Lefèvre, N. Mollaret
The Invertébrés Marins (Marine Invertebrates) collections of the Museum national d’Histoire naturelle (the French National Museum of Natural History; MNHN) are composed of about 1.5 million lots (containers of one or more specimens of the same taxonomic level) of at least 5 million specimens. The collections include a significant number of species from numerous geographical locations. Approximately 50,000 lots are types (sensu lato) with significant heritage value and covering the principal phyla and subphyla of marine invertebrates. Though the collections are mainly from marine environments, they also contain specimens from freshwater and terrestrial habitats. These organisms might be freeliving, parasitic, vagile, or sessile, and might live individually or form colonies; they display a great diversity of morphologies and sizes (Castro and Huber 2008). Collection management is carried out by a team of eight conservation technicians, assisted by four imaging and databasing technicians and several volunteers. The work is done in collaboration with fifteen scientific curators. The history of the MNHN began with the Jardin royal des plantes médicinales (Royal Garden of Medicinal Plants) created in 1635 by order of the King Louis XIII. In 1729, the pharmacy officially become the Cabinet d’histoire naturelle (Natural History Cabinet) (Laissus 1995). During the French Revolution, the National Convention reorganized the RoyalGarden (including theNatural History Cabinet) and transformed it into theMuseum of Natural History. In 1793, 12 chairs were established, including the “Lower Animals” (Animaux inférieurs) chair, assigned to Lamarck, who organized the first collections of marine invertebrates (Fischer-Piette 1944). From that time onward, the collections of marine invertebrates at the MNHN have been enhanced with specimens collected all over the world during oceanographic expeditions. The French tradition of marine scientific exploration started in the 18th century with several circumnavigation voyages (Bauchot et al. 1997) and continues today with diverse marine expedition programs led by MNHN research teams (MNHN website 2019). These programs, such as Tropical Deep-Sea Benthos (formerly MUSORSTOM), Our Planet Reviewed, and the Antarctic expeditions, cover a large part of the planet, aimed at exploring marine biodiversity (MNHN website 2021a, 2021b, 2021c). Current collection growth is from recent expeditions, but there are also noteworthy one-time contributions, including individual collecting events, donations, exchanges, and purchases. The Marine Invertebrate collections are housed in different locations at MNHN. Ninety percent of the collections are stored in the Zootheque (an underground building), under
法国国家自然历史博物馆(法国国家自然历史博物馆)的海洋无脊椎动物收藏;MNHN由至少500万个标本组成的约150万个批次(同一分类水平的一个或多个标本的容器)。这些藏品包括来自许多地理位置的大量物种。约有50,000件是具有重要遗产价值的类型(感知),涵盖了海洋无脊椎动物的主要门和亚门。虽然藏品主要来自海洋环境,但也包括淡水和陆地栖息地的标本。这些生物可能是自由生活的,寄生的,游动的,或无根的,可能单独生活或形成菌落;它们在形态和大小上表现出极大的多样性(Castro and Huber 2008)。藏品管理工作由一个由八名文物保护技术人员组成的小组进行,由四名成像和数据库技术人员以及几名志愿者协助。这项工作是与15位科学策展人合作完成的。MNHN的历史始于1635年根据路易十三国王的命令创建的皇家药用植物花园。1729年,药房正式成为自然历史柜(Cabinet d’histoire naturelle) (Laissus 1995)。在法国大革命期间,国民公会重组了皇家花园(包括自然历史柜),并将其改造成自然历史博物馆。1793年,设立了12个主席,其中“低等动物”主席被分配给拉马克,他组织了第一次海洋无脊椎动物的收集(Fischer-Piette 1944)。从那时起,MNHN收集的海洋无脊椎动物标本在世界各地的海洋考察中得到了加强。法国的海洋科学探索传统始于18世纪的几次环游航行(Bauchot et al. 1997),并在今天继续由MNHN研究团队领导的各种海洋探险项目(MNHN网站2019)。这些项目,如热带深海底栖生物(原MUSORSTOM)、我们的星球回顾和南极考察,覆盖了地球的大部分地区,旨在探索海洋生物多样性(MNHN网站2021a, 2021b, 2021c)。当前的收集增长来自最近的探险,但也有值得注意的一次性贡献,包括个人收集事件、捐赠、交换和购买。海洋无脊椎动物收藏被安置在MNHN的不同地点。百分之九十的藏品都存放在动物馆(地下建筑)
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引用次数: 0
Rehydration of Dried-Out Specimens: a New Approach 干枯标本复水化:一种新方法
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.73
F. Neisskenwirth
Different procedures are proposed in the literature for the rehydration of dried-out specimens. These procedures vary greatly in their efficiency and application. This work describes a new procedure that is inspired by the literature but that avoids heating the specimens. This method was applied to reconditioning dried-out specimens from a historical collection (Swiss freshwater fishes, bird brains, and bird eyes), stored at the Naturhistorisches Museum Bern in Switzerland. The procedure consists of five steps. The first step is the softening of hardened soft tissue with benzaldehyde and demineralized water. The second step is an indirect rehydration with water vapor. The third step is a chemically induced direct hydration using a trisodium phosphate solution that allows the specimen to swell in size before being washed with water to remove all additives. Finally, the rehydrated specimen is transferred into new preserving fluid. Because the dehydrating properties of ethanol as a preservative are problematic, this paper presents the results of an experimental case study using a glycerol solution as a preservation fluid.
文献中提出了不同的方法来处理干枯试样的再水化。这些程序在效率和应用方面差别很大。这项工作描述了一种新的程序,它受到文献的启发,但避免了加热标本。该方法被用于修复保存在瑞士伯尔尼自然历史博物馆(Naturhistorisches Museum)的历史收藏品(瑞士淡水鱼、鸟脑和鸟眼)中的干燥标本。这个过程包括五个步骤。第一步是用苯甲醛和去矿水软化硬化的软组织。第二步是用水蒸气间接再水合作用。第三步是化学诱导的直接水化,使用磷酸三钠溶液,在用水洗涤以去除所有添加剂之前,使样品膨胀。最后,将复水化后的标本转移到新的保存液中。由于乙醇作为防腐剂的脱水特性是有问题的,本文提出了使用甘油溶液作为保存液的实验案例研究的结果。
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引用次数: 0
Do not Dispose of Historic Fluid Collections: Evaluating Research Potential and Range of Use 不处理历史流体收集:评估研究潜力和使用范围
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.157
M. Herbin, M.D. Santin, R. Toro, K. Heuer, J. Cuisin, C. Martín, L. Raveendran, C. François, R. Debruyne
The use of specific preservative solutions by museum professionals to maintain fluid-preserved specimens has fluctuated over the years with advances in chemistry. The determining factors for the original choice of solution closely correlate with the historical parameters and original usage of the collections. Consequently, for any given collection, changes and substitutions over time in the types of preservative fluids used have likely occurred. The present comparative analysis of the state of brain preservation, carried out at macroscopic, microscopic, and molecular levels, allowed us to evaluate the effect of the different treatments applied over time to fluid-preserved collections. Our results confirm that the duration of formaldehyde exposure of the tissues clearly has an effect on their long-term preservation. Despite the controversies associated with the quality or use of some historic fixatives, modern analytical methods such as medical imagery reveal the preservation quality in historic specimens and their potential for future research use. However, the choice of fixatives and storage fluids to preserve the specimens is of critical importance because today's choices will influence the use of the specimen for advanced analytical methods in the future.
多年来,随着化学的进步,博物馆专业人员使用特定的防腐剂溶液来保存液体保存的标本的情况有所波动。初始解选择的决定因素与历史参数和集合的原始用途密切相关。因此,对于任何给定的采集,随着时间的推移,所使用的保存液体的类型可能会发生变化和替换。目前对大脑保存状态的比较分析,在宏观、微观和分子水平上进行,使我们能够评估随着时间的推移,不同处理对液体保存集合的影响。我们的研究结果证实,甲醛暴露于组织的持续时间显然对其长期保存有影响。尽管与一些历史固定剂的质量或使用有关的争议,现代分析方法,如医学图像,揭示了历史标本的保存质量及其未来研究使用的潜力。然而,选择固定液和储存液来保存标本是至关重要的,因为今天的选择将影响标本在未来先进分析方法的使用。
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引用次数: 1
The Dupuytren Anatomical and Pathological Collections: History and Complexity of the Wet Collections Dupuytren解剖和病理标本:湿标本的历史和复杂性
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.124
É. Quetel
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引用次数: 0
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