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Antibiotic sensitivity patterns and molecular detection of Enterotoxin genes in Staphylococcus aureus isolated from frozen muscle foods 冷冻肌肉食品中金黄色葡萄球菌的抗生素敏感性及肠毒素基因的分子检测
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.11
Oluwaseyi Akinware, Clement Olusola Ogidi, Bamidele Juliet Akinyele
Stapylococcus aureus is considered to be the most common pathogen in commonly consumed and improperly stored frozen foods. Hence, this study investigated the antibiotic sensitivity patterns and the occurrence of enterotoxin genes (sea and seb) in S. aureus isolated from frozen poultry meat of chicken, turkey and fish, namely Trachurus trachurus, Scomber scrombus and Merluccious merluccious. A total of 500 samples of raw meat from chicken (n = 130), turkey (n =130), and different fish (n =240) were randomly purchased from various markets and shopping malls in Akure between September 2020 and December 2021. The antibiotic sensitivity of isolated S. aureus was determined by disk diffusion method. S. aureus was analyzed for enterotoxin genes by multiplex polymerase chain reaction (PCR). The highest staphylococcal count (7.70 ×105 CFU/g) was observed in T. trachurus. One hundred and twenty five S. aureus were isolated from meat and fish with the highest occurrence (40.80%) in turkey meat. S. aureus is highly resistant (33.3 to 100%) to the tested antibiotics. The PCR assay, respectively, showed sea and seb coded for staphylococcal enterotoxin A and B. The presence of staphylococcal enterotoxin A and B in frozen muscle foods could be attributed to unhygienic conditions during processing and storage. The occurrence of antibiotic resistant and enterotoxigenic S. aureus in muscle foods could pose a serious threat to people when consumed. Hence, urgent attention is required to avert the incidences of staphylococcal food poisoning after consuming frozen meat and fish.
金黄色葡萄球菌被认为是最常见的病原体在普通消费和不正确储存的冷冻食品。因此,本研究对冷冻鸡肉、火鸡肉和鱼肉中分离的金黄色葡萄球菌(Trachurus Trachurus, Scomber scrombus和merlucious merlucious)的抗生素敏感模式和肠毒素基因(sea和seb)的发生情况进行了研究。在2020年9月至2021年12月期间,从阿库雷的各个市场和购物中心随机购买了500份鸡肉(n =130)、火鸡(n =130)和不同鱼类(n =240)的生肉样本。采用纸片扩散法测定分离金黄色葡萄球菌的抗生素敏感性。采用多重聚合酶链反应(PCR)检测金黄色葡萄球菌肠毒素基因。葡萄球菌计数最高的是气管t (7.70 ×105 CFU/g)。从肉类和鱼类中分离出125株金黄色葡萄球菌,其中以火鸡肉中检出率最高(40.80%)。金黄色葡萄球菌对测试的抗生素具有高度耐药性(33.3%至100%)。PCR检测结果显示,sea和seb分别编码葡萄球菌肠毒素A和B。冷冻肌肉食品中存在葡萄球菌肠毒素A和B可归因于加工和储存过程中的不卫生条件。肌肉食品中出现的耐抗生素和产肠毒素金黄色葡萄球菌可能对人们构成严重威胁。因此,迫切需要注意避免食用冷冻肉类和鱼类后发生葡萄球菌食物中毒。
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引用次数: 0
Effects of some fruits on the processing and composition of camel milk ice cream 几种水果对骆驼奶冰淇淋加工及成分的影响
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.07
Hassan Ali, Ibtisam E. M. El Zubeir
The present study was conducted to process and evaluate the chemical composition of ice cream made from camel milk, flavored with vanilla, baobab and papaya fruits. Camel milk, baobab and papaya were examined for chemical composition before the processing. Ice cream samples were made using one level of vanilla (3%) as control and two levels of each baobab and papaya (3% and 5%). Then ice cream samples were packed into plastic cups and stored in a freezer at -18 oC for 8 weeks. The chemical composition was examined every two weeks. The data showed significantly (P<0.05) higher total solids and ash in baobab (5%) ice cream samples, while high fat content was found in vanilla (3%) and papaya (5%). Also, high protein content was found in papaya ice cream samples. It was concluded that processing of ice cream using baobab (3%) and papaya (3%) gives ice cream with good chemical properties. The study recommended processing of ice cream from camel milk adding baobab and papaya fruits. Further studies should be conducted by adding local fruits and other additives to enhance the chemical properties of ice cream from camel milk as functional food.
本研究以骆驼奶为原料,用香草、猴面包树和木瓜果调味,对冰淇淋的化学成分进行了加工和评价。骆驼奶、猴面包树和木瓜在加工前进行了化学成分检测。冰淇淋样品使用一个水平的香草(3%)作为对照,猴面包树和木瓜各使用两个水平(3%和5%)。然后将冰淇淋样品装入塑料杯中,在-18摄氏度的冰箱中储存8周。化学成分每两周检查一次。数据显示,猴面包树(5%)冰淇淋样品中的总固体和灰分显著较高(P<0.05),而香草(3%)和木瓜(5%)中的脂肪含量较高。此外,木瓜冰淇淋样品中蛋白质含量也很高。研究表明,用猴面包树(3%)和木瓜(3%)加工冰淇淋,可以得到具有良好化学性能的冰淇淋。该研究推荐用骆驼奶添加猴面包树和木瓜果加工冰淇淋。应进一步研究添加当地水果和其他添加剂,以提高骆驼奶冰淇淋作为功能性食品的化学性能。
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引用次数: 0
Quality evaluation of pulp powder and the developed functional jam from African locust bean fruit 刺槐豆果浆粉及功能性果酱的品质评价
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.08
P. Akubor
The pulp powder was prepared from fruits of African locust bean tree and used to produce jam. The pulp and jam were analyzed for the chemical composition, physicochemical, microbiological and sensory properties. The total soluble solids of the pulp and jam were 25.5 and 72.5 oBrix, respectively. The pulp had pH of 5.3, while the pH of the jam was 3.3. The jam had higher total titratable acidity than the pulp. The jam contained higher crude protein (3.16 %), crude fat (1.03%), ash (4.3%) and carbohydrate (76.044%) contents than the pulp. The crude protein, fat, ash and carbohydrate contents of the pulp were 2.86, 0.85, 2.93 and 68.81%, respectively. The energy content of the jam was also higher than that of the pulp. The flavonoids, carotenoids, tannins, oxalates, phytates, saponins and alkaloids contents of the jam were lower than those of the pulp. The total sugars, reducing sugars, glucose, fructose and sucrose contents of the pulp were 7.7, 10.7, 5. 5, 4.8 and 5.8%, respectively, while those of the jam were 75.5, 47.6, 20.3, 30.3 and 21.68%, respectively. The vitamins B1, B2 and C contents of the pulp were 122, 35 and 35 mg/100g, respectively and decreased to 113, 10.2 and 22mg/100g, respectively, in the jam. The citric acid increased from 12.9 in the pulp to 15.6mg/100gin the jam. The P, K, Mg, Cu, Zn and Ca contents of the jam were higher than those of the pulp, while the Na and Fe contents of the pulp were higher than those of the jam. The total plate counts of the pulp and the pulp jam were 4.0 x104 and 1.2 x 101, respectively. The yeast and mould counts of pulp were 2.4 x104, while that of the jam was 0.3 x 101. The commercial plum jam was rated higher than the pulp jam for all the sensory attributes assessed except spreadability and overall acceptability.
以非洲刺槐豆树的果实为原料制备浆粉,用于制作果酱。对果肉和果酱进行了化学成分、理化、微生物和感官性能分析。果肉和果酱的可溶性固形物总量分别为25.5 oBrix和72.5 oBrix。果肉pH值为5.3,果酱pH值为3.3。果酱的总可滴定酸度高于果肉。果酱的粗蛋白质(3.16%)、粗脂肪(1.03%)、灰分(4.3%)和碳水化合物(76.044%)含量均高于果肉。果肉粗蛋白质、粗脂肪、粗灰分和粗碳水化合物含量分别为2.86、0.85、2.93和68.81%。果酱的能量含量也高于果肉。果酱中黄酮类、类胡萝卜素、单宁、草酸、植酸、皂苷和生物碱的含量均低于果肉。果肉总糖、还原糖、葡萄糖、果糖和蔗糖含量分别为7.7、10.7、5。分别为5、4.8、5.8%和75.5、47.6、20.3、30.3、21.68%。果肉中维生素B1、B2和C含量分别为122、35和35 mg/100g,果酱中维生素B1、B2和C含量分别降至113、10.2和22mg/100g。柠檬酸含量由果肉中的12.9 mg/100gin提高到果酱中的15.6mg/100gin。果酱的P、K、Mg、Cu、Zn和Ca含量高于果肉,果肉的Na和Fe含量高于果酱。纸浆和果酱的总板数分别为4.0 × 104和1.2 × 101。果肉酵母菌数为2.4 × 104,果酱酵母菌数为0.3 × 101。除涂抹性和总体可接受性外,商品梅酱的所有感官指标均高于纸浆果酱。
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引用次数: 0
Amino acid profile and protein quality of starter cultures fermented African Yam bean (Sphenostylis sternocarp) seed condiment 发酵非洲Yam豆(Sphenostylis sternocarp)种子调味品发酵剂的氨基酸组成和蛋白质质量
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.04
Pius Ifeanyi Okolie, Martins Ebunoluwa Itohan, Emilymary Chima Okolie, A. Obadina
African yam bean seeds condiment is an unpopular nutritive local food seasoning compared to Iru and is mostly produced by natural fermentation. Production with a defined starter culture will give a novel functional condiment with enhanced nutritive values, especially amino acids, to prevent hidden hunger, shorten the fermentation time, and improve product uniformity. The effect of different monoculture bacterial starters and natural fermentation on the amino acids profile of condiments produced from African yam bean seeds was investigated. The result revealed a total of 18 amino acids with glutamic and aspartic acids as the most abundant amino acids in all the samples portions, with a range of values of 9.79-11.44 g/100 g protein and 9.20 -10.67 g/100 g protein respectively. The inoculated samples were rich in all the amino acids quality than un-inoculated. The total amino acid content of the samples was significantly different (p ≤ 0.05). Bacillus amyloliquefacien starter fermented sample had the highest total amino acid value of 95.80 g/100 g protein while Bacillus subtilis fermented and un-inoculated seed portion samples had a total amino acid value of 86.81 g/100g and 74.04 g/100 g protein respectively. The essential amino acids (EAA10) were significantly different (p ≤ 0.05) and were in the range of 35.75-46.32 g/100 g protein with leucine, arginine, lysine, valine, isoleucine and phenylalanine the most abundant in inoculated and un-inoculated samples portions and were more concentrated in inoculated samples. Non-essential amino acids were slightly higher (38.29 - 49.47 g/100 g protein) than the total essential amino acids. The total acidic amino acids varied from 18.99 – 22.11 g/100 g protein and were significantly higher than the basic amino acids (12.88 – 15.95 g/100 g protein). The amino acid content of the Bacillus amyloliquefacien starter fermented sample was better enhanced than the sample fermented with Bacillus subtilis starter. The study concluded that controlled fermented condiments produced with B. amyloliquefacien culture boosted the amino acids value and profile.
与Iru相比,非洲山芋豆籽调味品是一种不受欢迎的营养性当地食品调味品,主要通过自然发酵生产。用确定的发酵剂培养生产将提供一种新的功能性调味品,具有更高的营养价值,尤其是氨基酸,以防止隐性饥饿,缩短发酵时间,提高产品的均匀性。研究了不同的单一培养细菌发酵剂和自然发酵对非洲山芋豆籽调味品氨基酸组成的影响。结果显示,在所有样品部分中,总共有18个氨基酸,其中谷氨酸和天冬氨酸是最丰富的氨基酸,其值范围分别为9.79-11.44g/100g蛋白质和9.20-10.67g/100g蛋白质。接种后的样品比未接种的样品富含所有氨基酸。样品的总氨基酸含量存在显著差异(p≤0.05)。解淀粉芽孢杆菌发酵剂样品的总氨基值最高,为95.80g/100g蛋白质,枯草芽孢杆菌发酵和未接种种子部分样品的总氨酸值分别为86.81g/100g和74.04g/100g蛋白。必需氨基酸(EAA10)差异显著(p≤0.05),在35.75-46.32g/100g蛋白质范围内,亮氨酸、精氨酸、赖氨酸、缬氨酸、异亮氨酸和苯丙氨酸在接种和未接种样品中含量最高,在接种样品中浓度更高。非必需氨基酸(38.29-49.47g/100g蛋白质)略高于总必需氨基酸。总酸性氨基酸在18.99–22.11 g/100 g蛋白质之间变化,显著高于碱性氨基酸(12.88–15.95 g/100 g蛋白)。解淀粉芽孢杆菌发酵剂发酵样品的氨基酸含量比用枯草芽孢杆菌发酵液发酵的样品提高得更好。研究得出结论,用解淀粉芽孢杆菌培养物生产的受控发酵调味品提高了氨基酸的价值和含量。
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引用次数: 0
Effects of sprouted soybeans and ginger on soymilk quality attributes via a developed dual-powered milk pasteurizer 通过研制的双动力牛奶巴氏杀菌器对豆浆品质特性的影响
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.03
Mayowa Saheed Sanusi, Musliu Olushola Sunmonu, Rafat Sani, Al Ameen Adeola Adesanya, Ameen Gegele Hammed, Abodunrin Tolulope
Soymilk market has achieved tremendous progress in consumption and sales due to its inexpensiveness and soymilk protein content. However, due to the present global health crisis, there is a need to further devise means of enriching the quality of soymilk. This study aimed to evaluate the effects of sprouted soybeans and ginger on soymilk quality and sensory attributes. A developed dual -powered milk pasteurizer was used to produce the soymilk. Soybeans, a mixture of soybeans and sprouted soybeans, and sprouted soybeans were interacted with peeled ginger and whole ginger rhizome using Taguchi experimental design to produce nine soymilk samples. The proximate composition, calorific value, total phenolic content and phytic acid content of the soymilk samples were determined using standard procedures. The moisture content of the soymilk samples ranged from 80.85 to 83.78%, crude protein content (7.47 – 9.54%), ash content (0.72 – 1.27%), fibre content (0.24 – 0.73%), crude fat (0.24 - 5.44%), carbohydrate content (1.29 -6.82%) and calorific value (67.6 kcal/g - 91.58 kcal/g). The total phenolic content and phytic acid content ranged from 4.81 mg/l to 11.88 GAE mg/l and 26.77 to 41.86 mg/l, respectively. The soymilk produced from the mixture of sprouted soybeans and un-sprouted soybeans with the addition of either whole or peeled ginger significantly increased the crude protein content, ash content, calorific value and total phenolic content to 9.54%, 1.27%, 91.58 kcal/g and 11.88 GAE mg/l, respectively. In addition, it increased the overall sensory acceptability of soymilk. Conclusively, the use of sprouted soybeans and the addition of ginger should be encouraged to promote a more enriched soymilk beverage. Also, the developed dual-powered milk pasteuriser could be adopted for soymilk production.
豆浆市场由于价格低廉和豆浆蛋白含量高,在消费和销售方面都取得了巨大的进步。然而,由于目前的全球健康危机,有必要进一步设计提高豆浆质量的方法。本试验旨在评价豆芽和生姜对豆浆品质和感官属性的影响。研制的双动力牛奶巴氏杀菌机用于豆浆的生产。采用田口试验设计,将大豆、大豆与豆芽的混合物、豆芽与去皮姜和全姜进行交互作用,生产9种豆浆样品。采用标准方法测定豆浆样品的近似组成、热值、总酚含量和植酸含量。豆浆样品的水分含量为80.85 ~ 83.78%,粗蛋白质含量为7.47 ~ 9.54%,灰分含量为0.72 ~ 1.27%,纤维含量为0.24 ~ 0.73%,粗脂肪含量为0.24 ~ 5.44%,碳水化合物含量为1.29 ~ 6.82%,发热量为67.6 ~ 91.58 kcal/g。总酚含量为4.81 ~ 11.88 mg/l,植酸含量为26.77 ~ 41.86 mg/l。以发芽大豆与未发芽大豆混合生产的豆浆中,添加全姜或去皮姜均显著提高了豆浆的粗蛋白质含量、灰分含量、发热量和总酚含量,分别达到9.54%、1.27%、91.58 kcal/g和11.88 GAE mg/l。此外,还提高了豆浆的整体感官接受度。最后,应该鼓励使用豆芽和添加生姜来促进更丰富的豆奶饮料。研制的双动力牛奶巴氏杀菌机可用于豆浆生产。
{"title":"Effects of sprouted soybeans and ginger on soymilk quality attributes via a developed dual-powered milk pasteurizer","authors":"Mayowa Saheed Sanusi, Musliu Olushola Sunmonu, Rafat Sani, Al Ameen Adeola Adesanya, Ameen Gegele Hammed, Abodunrin Tolulope","doi":"10.17508/cjfst.2023.15.1.03","DOIUrl":"https://doi.org/10.17508/cjfst.2023.15.1.03","url":null,"abstract":"Soymilk market has achieved tremendous progress in consumption and sales due to its inexpensiveness and soymilk protein content. However, due to the present global health crisis, there is a need to further devise means of enriching the quality of soymilk. This study aimed to evaluate the effects of sprouted soybeans and ginger on soymilk quality and sensory attributes. A developed dual -powered milk pasteurizer was used to produce the soymilk. Soybeans, a mixture of soybeans and sprouted soybeans, and sprouted soybeans were interacted with peeled ginger and whole ginger rhizome using Taguchi experimental design to produce nine soymilk samples. The proximate composition, calorific value, total phenolic content and phytic acid content of the soymilk samples were determined using standard procedures. The moisture content of the soymilk samples ranged from 80.85 to 83.78%, crude protein content (7.47 – 9.54%), ash content (0.72 – 1.27%), fibre content (0.24 – 0.73%), crude fat (0.24 - 5.44%), carbohydrate content (1.29 -6.82%) and calorific value (67.6 kcal/g - 91.58 kcal/g). The total phenolic content and phytic acid content ranged from 4.81 mg/l to 11.88 GAE mg/l and 26.77 to 41.86 mg/l, respectively. The soymilk produced from the mixture of sprouted soybeans and un-sprouted soybeans with the addition of either whole or peeled ginger significantly increased the crude protein content, ash content, calorific value and total phenolic content to 9.54%, 1.27%, 91.58 kcal/g and 11.88 GAE mg/l, respectively. In addition, it increased the overall sensory acceptability of soymilk. Conclusively, the use of sprouted soybeans and the addition of ginger should be encouraged to promote a more enriched soymilk beverage. Also, the developed dual-powered milk pasteuriser could be adopted for soymilk production.","PeriodicalId":10771,"journal":{"name":"Croatian journal of food science and technology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136017055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensory profiling of plantain-lima bean Momo (A Nigerian food pudding) using chemometric approach 大蕉-利马豆Momo(尼日利亚一种食品布丁)的化学计量学感官分析
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.06
Elizabeth Oluremi Farinde, Adekunbi Adetola Malomo, Omotola Mary Taiwo
Plantain and lima beans are important food crops with food nutrition security potentials in Nigeria. Plantain-lima bean momo is a nutrient-enriched pudding produced from unripe plantain and lima beans. The study aimed at evaluating the sensory profile of the plantain -lima bean momo to drive its consumers’ acceptability. Plantain and lima beans flour blends of various ratios were processed into momo. The momo samples were presented to panelists for sensory evaluation. Scores were based on nine point hedonic scale. Data obtained were subjected to Principal component and Agglomerative hierarchical clustering analysis. Results show that the addition of more than 20% of lima beans negatively affected the colour, appearance, flavour, texture and taste of the plantain-lima bean momo. PP (100% plantain flour momo) had the strongest positive correlation with appearance and texture. PA (90% unripe plantain flour + 10 % lima bean flour momo) was preferred mostly of all the momo samples in terms of overall acceptability. The principal component analysis (PCA) divided the sensory parameters of the momo samples into five factors with PC1 and PC2 accounting for 96.68% of the variance. Agglomerative hierarchical clustering (AHC) showed that PA and PP have similarities in all the sensory properties evaluated and that addition of 10% and 20% of lima beans to plantain flour did not have negative effect on the sensory characteristics of the product. Lima bean flour could be added to plantain flour up to 20% level for enhancement of momo nutritional quality, while still retaining its organoleptic qualities.
大蕉和青豆是尼日利亚具有粮食营养安全潜力的重要粮食作物。大蕉利马豆momo是一种营养丰富的布丁,由未成熟的大蕉和利马豆制成。该研究旨在评估芭蕉-利马豆momo的感官特征,以推动其消费者的接受度。将大蕉粉与青豆粉按不同比例混合制成馍馍。momo样品被提交给小组成员进行感官评估。得分是基于九分制的快乐量表。得到的数据进行主成分和聚类层次聚类分析。结果表明,添加量超过20%的青豆会对车前草-青豆momo的色、形、味、质、味产生负面影响。PP(100%大蕉粉momo)与外观和质地的正相关最强。在所有momo样品中,PA(90%生车前草粉+ 10%利马豆粉momo)的总体接受度最高。主成分分析(PCA)将momo样品的感官参数分为5个因子,其中PC1和PC2占方差的96.68%。聚类层次聚类分析(AHC)表明,PA和PP在所有感官性能评价上具有相似性,在大蕉粉中添加10%和20%的利马豆对产品的感官特性没有负面影响。在大蕉粉中添加20%的利马豆粉,可在保持大蕉粉感官品质的前提下提高大蕉粉的营养品质。
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引用次数: 0
Extraction of phenolics from Azadirachta indica flowers 印楝花中酚类物质的提取
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.05
G. Janarny, Kamburawala Kankanamge Don Somathilaka Ranaweera, Katugampalage Don Prasanna Priyantha Gunathilake
Azadirachta indica is a well-known tree for the medicinal use of its leaves, barks and flowers. It has been identified as a rich source of phenolics which attribute to its medicinal properties. However, these phenolics should be extracted appropriately to effectively utilize them in various applications. This study focused on maximizing the extraction yield of total phenolics and total anthocyanins along with maximum 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity from edible flowers of A. indica as a function of solid:liquid ratio, ethanol concentration, extraction temperature and time. The data was subjected to response surface methodology and the corresponding second order polynomial models were generated. The results showed that the polynomial models for all responses were significant, did not show lack of fit and presented determination of coefficients above 95%. This indicates the suitability of the models for prediction purposes. Using desirability function, the optimum extraction process parameters to obtain maximum values of all responses were found to be solid:liquid ratio 1:32.2, ethanol concentration 42.72%, extraction temperature 55 °C and time 39.25 minutes. Under these optimal conditions, the experimental values for total phenolic content was 85.44±2.41 mg gallic acid equivalents per gram of dry weight, total anthocyanin content was 44.32±2.75 µg cyanidin-3-glucoside per gram of dry weight and DPPH radical scavenging activity was 31.43±1.67%. These values were in close agreement with the predicted values and did not show any significant difference (p<0.05). The identified optimized conditions could be used to extract phenolics from A. indica flowers in a cost effective and efficient manner.
印楝因其叶、树皮和花朵的药用价值而闻名。由于其药用特性,它已被确定为酚类物质的丰富来源。然而,这些酚类物质应该被适当地提取,以便在各种应用中有效地利用它们。本研究旨在通过固液比、乙醇浓度、提取温度和时间的函数,最大限度地提高籼稻食用花中总酚类和总花青素的提取率,以及最大限度地清除2,2-二苯基-2-苦基肼(DPPH)自由基的活性。数据采用响应面方法,并生成相应的二阶多项式模型。结果表明,所有响应的多项式模型都是显著的,没有表现出拟合不足,并且系数的确定率超过95%。这表明模型适用于预测目的。使用期望函数,获得所有响应最大值的最佳提取工艺参数为固液比1:32.2,乙醇浓度42.72%,提取温度55°C,时间39.25分钟。在这些最佳条件下总酚含量的实验值为85.44±2.41mg没食子酸当量/克干重,总花青素含量为44.32±2.75µg矢车菊素-3-葡萄糖苷/克干重,DPPH自由基清除活性为31.43±1.67%。这些值与预测值非常一致,没有任何显著差异(p<0.05)。所确定的优化条件可用于从A.indica花中提取酚类物质,具有成本效益和高效性。
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引用次数: 0
Effect of varietal differences on chemical and pasting properties of composite wheat-cassava flours produced from low postharvest physiologically deteriorated cassava roots (Manihot esculenta Crantz) 品种差异对采后低生理变质木薯根生产小麦-木薯复合粉化学和糊化特性的影响
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.09
John Praise Alimi, Samuel Aondoaver Ahemen, Janet Omolola Alimi, Peter Oshiapi Iluebbey
This study investigated the effect of varietal differences on the chemical and pasting properties of composite wheat-cassava flours produced with blend of wheat and high-quality cassava flour from low postharvest physiologically deteriorated (PPD) cassava roots. Wholesome four varieties of yellow-fleshed low PPD cassava roots and one variety of high PPD cassava root were, peeled, washed, grated, pressed, pulverized, flash dried at 120 °C for 8 minutes, milled with cyclone hammer mill fitted with a screen of 250 µm aperture size, cooled and packed into high density polyethylene bag. The high-quality cassava flours (HQCF) were composited with wheat flour and analyzed for chemical and pasting properties. Data obtained were subjected to analysis of variance (ANOVA) using Statistical Package for Social Sciences (SPSS version 25.0) and significant means were separated applying Duncan multiple range test and Pearson’s correlation was also determined. The results depict that moisture, protein, fat, ash, sugar, starch, dry matter and energy value ranged from 12.45 - 13.10 %, 10.30 - 11.65 %, 0.40 - 3.71 %, 0.58 - 0.62 %, 1.75± - 2.08 %, 70.94 - 74.40 %, 86.90 - 87.55 % and 1464.29 - 1531.66 KJ/kg, respectively. Peak, trough, breakdown, final, setback, peak time and pasting temperature ranged from 180.63 - 247.21 RVU, 95.84 - 129.00 RVU, 83.42 - 118.21 RVU, 139.29 - 253.71 RVU, 83.79 - 124.71 RVU, 5.90 - 6.17 min and 69.00 - 73.48 °C, respectively. Considering the relatively high dry matter content, energy value, peak viscosity, low breakdown viscosity and pasting time, flour blends C-C1368-W and C-C0593-W prepared with HQCFs from IITA-TMS-IBA-011368 and IITA-TMS-IBA-070593 cassava varieties could find application in baking and confectionery industry.
研究了品种差异对小麦与优质采后生理变质木薯根粉混合制成的小麦-木薯复合粉的化学和糊化性能的影响。将健康的4个品种低PPD黄瓤木薯根和1个品种高PPD木薯根,经去皮、洗涤、磨碎、压榨、粉碎、120℃闪干8分钟后,用孔径为250µm筛网的旋流锤式粉碎机粉碎,冷却后装入高密度聚乙烯袋中。将优质木薯粉与小麦粉进行复合,并对其化学性能和糊化性能进行了分析。所得数据采用SPSS 25.0版本进行方差分析(ANOVA),采用Duncan多元极差检验分离显著性均值,并进行Pearson相关分析。结果表明:水分、蛋白质、脂肪、灰分、糖、淀粉、干物质和能量值分别为12.45 ~ 13.10%、10.30 ~ 11.65%、0.40 ~ 3.71%、0.58 ~ 0.62%、1.75±2.08%、70.94 ~ 74.40%、86.90 ~ 87.55%和1464.29 ~ 1531.66 KJ/kg。峰、谷、击穿、末、退峰、峰时间和粘贴温度分别为180.63 ~ 247.21 RVU、95.84 ~ 129.00 RVU、83.42 ~ 118.21 RVU、139.29 ~ 253.71 RVU、83.79 ~ 124.71 RVU、5.90 ~ 6.17 min和69.00 ~ 73.48℃。以IITA-TMS-IBA-011368和IITA-TMS-IBA-070593木薯品种hqcf制备的C-C1368-W和C-C0593-W面粉混合物具有较高的干物质含量、能量值、峰值粘度、较低的破碎粘度和糊化时间,可用于烘焙和糖果行业。
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引用次数: 0
Drying parameters of wild lettuce (Lactuca taraxaxifolia L) affected by different drying methods 不同干燥方式对野生莴苣干燥参数的影响
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.13
Clement Adesoji Ogunlade, Simeon Olatayo Jekayinfa, Olusola Janet Oyedele, Ajekigbe Sola Olajire, Abigael Oluseye Oladipo
This study focused on the effect of drying method on drying properties of wild lettuce leaves. The fresh wild lettuce was washed thoroughly to remove extraneous parts and subjected to pretreatments by dipping 200 g of vegetable samples into the water (1.5w/v) solution with 0.3% sodium metabisulphite held at room temperature. The pre-treated and untreated leaves were weighed and loaded in stainless steel tray and subjected to drying under cabinet dryer, open-sun drying and solar dryer. The wild lettuce drying curve exhibits a gentle downward curve, i.e. a high moisture loss at the early period of drying for all the drying methods used. The effective moisture diffusivity and activation energy were determined. The effective moisture diffusivity increased with the increase in temperature of drying. This study showed that post harvest losses of wild letuce can be minimized through drying, using mechnical devices or open sun drying, which is one of the oldest preservation processes available to the mankind.
研究了不同干燥方式对野生生菜叶片干燥特性的影响。将新鲜的野生生菜彻底洗净,去除多余的部分,将200 g蔬菜样品浸入含有0.3%焦亚硫酸钠的水(1.5w/v)溶液中,在室温下进行预处理。将预处理和未处理的叶片称重装入不锈钢托盘,分别在柜式干燥机、露天干燥机和太阳能干燥机下进行干燥。野生生菜的干燥曲线呈平缓的向下曲线,即在干燥初期,无论采用何种干燥方法,其水分损失都很大。测定了有效水分扩散系数和活化能。有效水分扩散系数随干燥温度的升高而增大。本研究表明,通过机械干燥或露天晒干,可以最大限度地减少野生莴苣收获后的损失,这是人类最古老的保存方法之一。
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引用次数: 0
Impact of extraction method on alkaloid content and antioxidant activity of Matricaria pubescens 提取方法对短毛药材生物碱含量及抗氧化活性的影响
Pub Date : 2023-06-14 DOI: 10.17508/cjfst.2023.15.1.10
Hassiba Metrouh-Amir, Nadir Amir
Matricaria pubescens is a medicinal plant much appreciated by the people of the Saharan regions and widely used to treat many diseases. The present study was devoted to evaluate the effect of extraction technique, pH and nature of solvent on alkaloid content and antioxidant activity of M. pubescens. Alkaloid extraction was carried out by applying two techniques, maceration extraction and Soxhlet extraction, using several solvents at different pH (alkaline chloroform, acid methanol, neutral methanol and neutral ethanol). In the present study, the antioxidant activity of the alkaloid extracts of M. pubescens was evaluated using ferric reducing power, molybdate reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, β-carotene bleaching assay, hydroxyl radical scavenging activity (assessed by deoxyribose assay and HOSC assay) and oxygen radical absorbance capacity (ORAC). The obtained results show that the extraction rates obtained and the antioxidant activities of alkaloid extracts were affected by the extraction technique, pH and the solvent used. The highest extraction rates were obtained with the ethanolic neutral (Soxhlet) and methanolic acidified (maceration) extracts. The alkaloid extracts of M. pubescens exerted a reducing activity of ferric and molybdate ions. They also showed significant radical scavenger activity against DPPH, ABTS, hydroxyl, and peroxyl radicals. This significant wealth of alkaloids and these various antioxidant activities could justify the traditional use of M. pubescens.
短毛母属是撒哈拉地区人民喜爱的药用植物,被广泛用于治疗多种疾病。研究了提取工艺、提取液pH和提取溶剂性质对毛毛中生物碱含量和抗氧化活性的影响。采用浸渍提取和索氏提取两种工艺,采用不同pH的溶剂(碱性氯仿、酸性甲醇、中性甲醇和中性乙醇)进行生物碱提取。本研究采用铁还原力、钼酸盐还原力、DPPH自由基清除能力、ABTS自由基清除能力、β-胡萝卜素漂白试验、羟基自由基清除能力(脱氧核糖体试验和HOSC试验)和氧自由基吸收能力(ORAC)来评价毛缕草生物碱提取物的抗氧化活性。结果表明,提取工艺、pH和溶剂对生物碱提取物的提取率和抗氧化活性均有影响。乙醇中性(索氏)和甲醇酸化(浸渍)提取物提取率最高。短毛霉生物碱提取物对铁离子和钼酸盐具有还原活性。它们对DPPH、ABTS、羟基和过氧基自由基也有明显的清除活性。这种丰富的生物碱和各种抗氧化活性可以证明毛蕊草的传统用途是合理的。
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引用次数: 0
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Croatian journal of food science and technology
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