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Cryopreservation of Drosophila melanogaster embryonic nuclei in a Dimethyl sulfoxide (ME2SO)-free solution 黑腹果蝇胚胎核在无二甲亚砜（ME2SO）溶液中的低温保存
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-10 DOI: 10.1016/j.cryobiol.2025.105561
Troy Louwagie , Lindsey G. O’Brien , Daryl M. Gohl , Allison Hubel
Drosophila melanogaster is a well-known organism for biological research. With more than 150,000 live stocks maintained, there is an urgent need for cryopreservation of Drosophila stocks to limit maintenance costs and genetic drift. Two methods have been described for freezing Drosophila stocks: vitrification of embryos and of primordial germ cells. These methods show success in cryopreserving stocks, but the robustness and the applicability of these approaches in preserving a wide range of mutant stocks has not been established. Here, we describe a method for cryopreserving isolated embryonic nuclei using a slow-cooling method. Using a differential evolution algorithm, we identified five different formulations that show >80% recovery and used Raman spectroscopy to image nuclei at low temperatures. Finally, we demonstrate that cryopreserved nuclei retain biological function through an induced hsp70 transcriptional response. This work represents steps towards cryopreserving nuclei with the ultimate goal of regenerating stocks by embryonic nuclear transplantation.
黑腹果蝇是生物学研究中著名的生物。由于维持了超过150,000个活的果蝇种群，因此迫切需要对果蝇种群进行低温保存，以限制维持成本和遗传漂变。已经描述了两种冷冻果蝇种群的方法：胚胎玻璃化和原始生殖细胞玻璃化。这些方法在冷冻保存中取得了成功，但这些方法在保存大范围突变体砧木方面的稳健性和适用性尚未建立。在这里，我们描述了一种使用缓慢冷却方法冷冻保存离体胚胎核的方法。使用差分进化算法，我们确定了五种不同的配方，显示80%的回收率，并使用拉曼光谱在低温下对原子核进行成像。最后，我们证明低温保存的细胞核通过诱导hsp70转录反应保留了生物学功能。这项工作代表了细胞核低温保存的最终目标是通过胚胎核移植再生砧木的步骤。
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引用次数: 0
Development of B cell medicine cryopreservation process B细胞药物冷冻保存工艺的研究进展
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105366
Mark Lalli, Jordan Smelker, Rashmi Kshirsagar
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引用次数: 0
Improving non-contact cooling rate with liquid nitrogen by hierarchical surface structures 利用分层表面结构提高液氮非接触冷却速率
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105371
Meng Shi
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引用次数: 0
Permeable and non-permeable cryoprotectant-free cryopreservation of larger volumes human semen 大容量人精液的透性和不透性无冷冻保护剂冷冻保存
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105359
Karekin D. Esmeryan , Todor A. Chaushev
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引用次数: 0
Oocyte and embryo vitrification using automated equipment based on microfluidic chip 基于微流控芯片的自动化设备对卵母细胞和胚胎进行玻璃化冷冻
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105360
Jing Shen , Yukun Cao , Weijie Li , Xinli Zhou
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引用次数: 0
HIFU enables structural preservation in cryopreserved vascular tissue: Insights from electron microscopy HIFU能够在冷冻保存的血管组织中进行结构保存：来自电子显微镜的见解
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105395
Enrique Alcalá , Virginia Armas , Laura Encabo , Carmen Leiva , Juan José Jiménez , Emil Kendziorr , Ramón Risco
{"title":"HIFU enables structural preservation in cryopreserved vascular tissue: Insights from electron microscopy","authors":"Enrique Alcalá ,&nbsp;Virginia Armas ,&nbsp;Laura Encabo ,&nbsp;Carmen Leiva ,&nbsp;Juan José Jiménez ,&nbsp;Emil Kendziorr ,&nbsp;Ramón Risco","doi":"10.1016/j.cryobiol.2025.105395","DOIUrl":"10.1016/j.cryobiol.2025.105395","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105395"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Granulosa cell-derived extracellular vesicles modulate oxidative stress in vitrified bovine oocytes 颗粒细胞衍生的细胞外囊泡调节玻璃化牛卵母细胞的氧化应激
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105374
Judith Diaz-Muñoz , Sonia Gago , Yulia N. Cajas , Karina Cañón-Beltrán , Manel López-Béjar , Dimitrios Rizos , Teresa Mogas
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引用次数: 0
Exploring the potential of lea protein - Solute interactions for stabilizing biomolecules during drying 探索蛋白质-溶质相互作用在干燥过程中稳定生物分子的潜力
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105345
Anja Thalhammer
{"title":"Exploring the potential of lea protein - Solute interactions for stabilizing biomolecules during drying","authors":"Anja Thalhammer","doi":"10.1016/j.cryobiol.2025.105345","DOIUrl":"10.1016/j.cryobiol.2025.105345","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105345"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Challenges and advances in the cryopreservation of Paracentrotus lividus eggs 卵黄旁突鱼卵冷冻保存的挑战与进展
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105396
Sara Campos, Estefanía Paredes, Jesús Troncoso
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引用次数: 0
Isochoric freezing: Optimizing food preservation through controlled temperature and pressure conditions 等时冷冻：通过控制温度和压力条件来优化食品保存
IF 2.1 3区 生物学 Q2 BIOLOGY
Cryobiology
Pub Date : 2025-12-01 DOI: 10.1016/j.cryobiol.2025.105370
Alan L. Maida , Anthony N. Consiglio , Cristina Bilbao-Sainz , Andrew Karman , Gary Takeoka , Matthew J. Powell-Palm , Boris Rubinsky
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引用次数: 0
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