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Formulation of Fruit-Based Probiotic Drink From Snake Fruit (Salacca Zalacca) and Lactiplantibacillus plantarum subsp. plantarum Dad-13 以蛇果和植物乳杆菌亚种为原料制备水果益生菌饮料。杆菌Dad-13
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.26
Achmad Nur Syawal Alwi, E. Rahayu, Tyas Utami, R. Yanti, D. Suroto
Snake fruit contains monosaccharides, mainly fructose and glucose, which are indispensable substrates for the growth factors of probiotic bacteria. Therefore, this study aims to develop the Fermented Snake Fruit Juice (FSFJ) using the local probiotic bacteria Lactiplantibacillus plantarum subsp. plantarum Dad-13. The results showed that the optimal fermentation time was 24 hours, with a viable cell count of 2.7×108, pH 3.77, and total acid of 0.33%. The glucose and fructose content in FSFJ were decreased during fermentation. The addition of sucrose at 0%, 3%, and 6% showed that different sucrose concentrations were statistically insignificant to the viable cell count, pH, and total acid. A hedonic test was conducted, where the sample with a 6% sucrose level was the most preferred by the panelists hence, deemed as the best formulation. Furthermore, the optimal formulation sample was stored at 4°C for 30 days, and the result indicated that the viable cell count did not present a significant difference. The pH value was decreased from 3.68 to 3.60 and the total acid was increased from 0.42% to 0.56%. The volatile compounds of FSFJ were dominated by compounds responsible for snake fruit character, such as methyl 4-methyl-2-pentanoate and methyl β-methyl valerate, with some fermentation-related volatile compounds. In conclusion, Snake Fruit Juice (SFJ) is a suitable carrier medium for probiotic bacteria and remains of sufficient quality after 30 days of cold storage.
蛇果中含有以果糖和葡萄糖为主的单糖,是益生菌生长因子不可缺少的底物。因此,本研究旨在利用当地益生菌植物乳杆菌亚种开发发酵蛇果汁(FSFJ)。杆菌Dad-13。结果表明,最佳发酵时间为24 h,活菌数为2.7×108, pH为3.77,总酸为0.33%。发酵过程中,FSFJ中葡萄糖和果糖含量降低。蔗糖添加量为0%、3%和6%时,不同蔗糖浓度对活细胞数、pH和总酸的影响均无统计学意义。进行了享乐测试,其中含有6%蔗糖水平的样品是小组成员最喜欢的,因此被认为是最佳配方。将最佳配方样品在4℃下保存30 d,结果表明,活性细胞计数无显著差异。pH值由3.68降低到3.60,总酸由0.42%提高到0.56%。FSFJ的挥发性成分以4-甲基-2-戊酸甲酯和β-戊酸甲酯等与蛇果性状有关的化合物为主,还有一些与发酵有关的挥发性成分。综上所述,蛇果汁是一种适宜的益生菌载体培养基,冷藏30 d后仍能保持足够的品质。
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引用次数: 0
Ethanolic Extract of Black Rice ‘Sembada Hitam’ Bran Protects the Cytotoxic Effect of H2O2 on NIH3T3 Cells. 黑米糠醇提物保护H2O2对NIH3T3细胞的细胞毒作用。
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.29
Galuh Oktavya, Y. A. Purwestri, H. T. Saragih, A. Nuriliani
Oxidative stress which is triggered by endogenous and exogenous stressors such as oxygen metabolism in mitochondria, radiation, drugs, and pollutants, negatively affect biological systems. Various pathophysiological conditions and the life span of organisms were affected by such condition. Secondary metabolites found in natural ingredients such as black rice have high antioxidant activity that can prevent oxidative stress. This study aimed to examine potency of the ethanolic extract of black rice (Oryza sativa L. 'Sembada Hitam') bran to protects H2O2-induced NIH3T3 cells. This research was focused to evaluate the potency of black rice bran’s (BRB’s) extract on cytotoxicity, apoptosis, and cell growth due to H2O2 induction. This study used a combination of H2O2 exposure at various concentrations (50, 100, 150, 200, and 300 μM) and BRB’s extract at various concentrations (7.81; 15.63; 31.25; 62.5; 125; 250; 500; and 1000 μg/mL). Our results showed that BRB’s extract at the concentration of 7.81 to 1000 μg/mL maintained NIH3T3 cells viability above 80% against 50 and 100 μM H2O2 exposure for 24 hours. These were in line with the apoptosis test results, which showed that the BRB’s extract suppressed apoptosis, especially the combination of BRB and H2O2 exposure at 62.5 μg/mL and 100 μM; 62.5 μg/mL and 200 μM, as well as 250 μg/mL and 100 μM, respectively. Moreover, the H2O2-induced NIH3T3 cells’ growth was maintained up to the fifth day under the BRB’s extract treatment. The result proved that pretreatment of BRB’s extract at the concentration of 62.5 μg/mL is highly effective as an anti-apoptotic and increases cell proliferation up to the fifth day on H2O2-induced NIH3T3 cells. Collecting all the results together, we suggested that BRB’s extract have a protective effect by maintaining NIH3T3 cell viability against H2O2 induction.
氧化应激是由线粒体中的氧代谢、辐射、药物和污染物等内源性和外源性应激源引发的,对生物系统产生负面影响。各种病理生理条件和生物体的寿命都受到这些条件的影响。在黑米等天然成分中发现的次生代谢物具有很高的抗氧化活性,可以防止氧化应激。本研究旨在考察黑米(Oryza sativa L.)乙醇提取物的效力。“Sembada Hitam”)麸皮保护h2o2诱导的NIH3T3细胞。本研究旨在探讨黑米糠提取物在H2O2诱导下对细胞毒性、细胞凋亡和细胞生长的影响。本研究采用不同浓度(50、100、150、200和300 μM)的H2O2暴露和不同浓度(7.81;15.63;31.25;62.5;125;250;500;1000 μg/mL)。结果表明,在浓度为7.81 ~ 1000 μg/mL的浓度下,暴露于50 μM和100 μM的H2O2下24 h, NIH3T3细胞的存活率维持在80%以上。这与细胞凋亡实验结果一致,表明BRB提取物对细胞凋亡有抑制作用,特别是在62.5 μg/mL和100 μM浓度下BRB与H2O2联合暴露;62.5 μg/mL和200 μM, 250 μg/mL和100 μM。此外,在BRB提取物处理下,h2o2诱导的NIH3T3细胞的生长可以维持到第5天。结果表明,62.5 μg/mL浓度预处理的白芍提取物对h2o2诱导的NIH3T3细胞具有良好的抗凋亡作用,可促进细胞增殖至第5天。综上所述,我们认为BRB提取物具有保护NIH3T3细胞免受H2O2诱导的作用。
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引用次数: 0
Eating Habits and Diet Diversity of Saudi Arabia Residents during the COVID-19 locked-down COVID-19封锁期间沙特阿拉伯居民的饮食习惯和饮食多样性
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.9
A. Alzaben, A. Al-Masud, Reham M. Gaid, Elham F. Almahmoud, Abdullah F. Alghannam, Arwa S. Altalhi, Shaima A. Alothman
Background: The COVID-19 pandemic has had a negative impact on overall health and well-being. Therefore, certain preventive measures may affect many lifestyle habits. This study aimed to explore the eating habits, food variety score (FVS), and diet diversity score (DDS) of adults residing in Saudi Arabia during the mandatory stay-at-home advisory for COVID-19. Methods: A cross sectional study was targeted adults living in Saudi Arabia during the COVID-19 lockdown (May – June 2020). An electronic self-reported survey was conducted through Research Electronic Data Capture (Redcap) distributed on social media platforms. The Arabic version of the questionnaire was previously evaluated for its reliability and validity. A total of 359 individuals who completed the food frequency questionnaire were included in the study. Diet diversity score (DDS) and food variety score (FVS) was calculated. Results: The study found that the distribution of FVS ranged between 4-27 food items. The distribution of DDS ranged between 3-14 food items. Income, working status, and living status were factors associated with the FVS and/or DDS. The majority of the cohort (>67%) had at least one snack/day and < 3 meals/day. Conclusion: Diet diversity was acceptable among Saudi adults during the COVID-19 lockdown period. Age, sex, having children, marital status, education level, and income were factors associated with diet diversity and eating habits (having breakfast, skipping meals, and number of snacks).
背景:2019冠状病毒病大流行对整体健康和福祉产生了负面影响。因此,某些预防措施可能会影响许多生活习惯。本研究旨在探讨在COVID-19强制居家咨询期间居住在沙特阿拉伯的成年人的饮食习惯、食物多样性评分(FVS)和饮食多样性评分(DDS)。方法:一项横断面研究针对在2019冠状病毒病封锁期间(2020年5月至6月)居住在沙特阿拉伯的成年人。通过研究电子数据捕获(Redcap)在社交媒体平台上分发了一项电子自我报告调查。先前对调查表的阿拉伯文本的信度和效度进行了评价。共有359名完成了食物频率调查问卷的人参与了这项研究。计算饮食多样性评分(DDS)和食物多样性评分(FVS)。结果:研究发现,FVS的分布范围在4 ~ 27种食品之间。DDS分布在3-14种食物之间。收入、工作状态和生活状态是与FVS和/或DDS相关的因素。大多数队列(>67%)每天至少吃一次零食,每天少于3顿饭。结论:在COVID-19封锁期间,沙特成年人的饮食多样性是可以接受的。年龄、性别、是否有孩子、婚姻状况、教育程度和收入是与饮食多样性和饮食习惯(吃早餐、不吃正餐和零食数量)相关的因素。
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引用次数: 1
Using Sweet Lupine Powder as a Healthy Supplement for the Production of Functional Chicken Burger in Jordan 用甜羽扇豆粉作为健康添加剂在约旦生产功能性鸡肉汉堡
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.11
Sati Y. Al –Dalain, M. Haddad, Samer Y. Alqaraleh
Chicken meat has grown more popular in recent years because of its great nutritional content,. It has a high protein content as well as a low-fat content and a high percentage of unsaturated fatty acids (polyunsaturated), which have a beneficial effect on consumers' health. Sweet lupine is a leguminous bean of the Fabaceae family. In this study, different proportions of powdered sweet lupine (10, 20, and 35 percent of the mix) were utilized to complement the chicken burgers that were produced in Jordan. The sample enriched with 20% sweet lupine (LB20) was rated as the most popular. The protein level of sweet lupine was 1.3 times that of chicken meat, but the fat amount was similar. The ash concentration of sweet lupine was 2.5 times greater, whereas the fiber content was 55 times higher. The overall carbs content of sweet lupine was 75 times more than that of chicken meat. When total amino acids were calculated, it was approximately the same. The ratio of SFA/USFA assured such finding that was higher with 1.37-fold rather than that of un-supplemented one. The twenty percent addition of sweet lupine as a supplement to chicken burgers gave the best sensory properties and less oxidized fat as seen in when TBARS test.
近年来,鸡肉因其丰富的营养成分而越来越受欢迎。它的蛋白质含量高,脂肪含量低,不饱和脂肪酸(多不饱和脂肪酸)的比例高,对消费者的健康有益。甜羽扇豆是豆科的一种豆科豆子。在这项研究中,不同比例的甜羽扇豆粉(10%,20%和35%的混合物)被用来补充在约旦生产的鸡肉汉堡。富含20%甜羽扇豆素(LB20)的样品被评为最受欢迎的。甜羽扇豆的蛋白质含量是鸡肉的1.3倍,但脂肪含量相近。甜羽扇豆的灰分浓度高2.5倍,纤维含量高55倍。甜羽扇豆的总碳水化合物含量是鸡肉的75倍。当计算总氨基酸时,结果大致相同。SFA/USFA的比值比未添加的高1.37倍。在鸡肉汉堡中添加20%的甜羽扇豆氨酸可以提供最好的感官特性和更少的氧化脂肪在TBARS测试中可以看到。
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引用次数: 0
Nutritional and Functional Quality of Mustard and Coriander Greens at Different Phases of Development 不同发育阶段芥菜和香菜的营养和功能品质
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.10
Manika Das, A. Dhar
Micronutrient deficiencies are a significant global health issue. More than 2 billion individuals worldwide are estimated to still be lacking in important vitamins and minerals today. The majority of these folks reside in developing countries and typically lack many micronutrients. Microgreens are immature edible vegetables has lots of potential health benefits, can grown easily. Mustard (Brassica nigra) and coriander (Corindrum sativum) greens are very common in India and can grow in Indian climate. People of both urban and rural population can afford and grow these.The aim of the present study is to analyse nutritional and functional quality of mustard and coriander greens at different phases of development. For this mustard and coriander greens were grown and collected at different phases of development (microgreen, babygreen and maturegreen) and their nutritional (macronutrients & micronutrients) and phyto-chemicals analysis were performed. Functional properties (anti-oxidant & anti-microbial) of mustard and coriander green were also evaluated.Result showed that micronutrients like beta carotene (66%), ascorbic acid(>100%), iron( 100%), calcium (28%), phosphorus(47%) level were higher in mustard microgreen compared to the mature green. In case of coriander green also micronutrients like beta carotene (86%), ascorbic acid (>100%), iron (90%), calcium (15% ), phosphorus(34%) level were also higher in microgreen compared to mature green. Mustard and coriander microgreens also showed potential anti-microbial activity against Escarechia coli and Bacillus subtilis.Being a dense source of nutrients and phyto-chemicals mustard and coriander microgreens may be considered as super food and can be used to combat malnutrition and major disorders that occur in the human body.
微量营养素缺乏是一个重大的全球健康问题。据估计,目前全球仍有超过20亿人缺乏重要的维生素和矿物质。这些人中的大多数居住在发展中国家,通常缺乏许多微量营养素。微型蔬菜是未成熟的可食用蔬菜,有很多潜在的健康益处,可以很容易地种植。芥菜(Brassica nigra)和香菜(Corindrum sativum)在印度很常见,可以在印度的气候下生长。城乡人口都能负担得起并种植这些作物。本研究的目的是分析芥菜和香菜在不同发育阶段的营养和功能品质。为此,在不同的发育阶段(微绿、幼绿和成熟绿)种植和收集芥菜和香菜,并对它们的营养(宏量营养素和微量营养素)和植物化学物质进行分析。对芥菜和香菜绿的抗氧化和抗微生物功能特性进行了评价。结果表明,芥菜微绿中β -胡萝卜素(66%)、抗坏血酸(>100%)、铁(100%)、钙(28%)、磷(47%)等微量元素含量均高于成熟绿。在香菜绿中,微绿中的微量营养素如-胡萝卜素(86%)、抗坏血酸(>100%)、铁(90%)、钙(15%)、磷(34%)含量也高于成熟绿。芥菜和香菜微蔬菜对大肠杆菌和枯草芽孢杆菌也有潜在的抑菌活性。芥菜和香菜是营养物质和植物化学物质的密集来源,可以被认为是超级食物,可以用来对抗营养不良和发生在人体中的主要疾病。
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引用次数: 0
Covid-19 Era in the Coastal Areas: The Cookies Formulation Gonad of Diadema setosum and its Impacts on Malnutrition During Infection 沿海地区新冠肺炎时代:凝固水肿的饼干配方性腺及其对感染期间营养不良的影响
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.16
W. Salma, A. Fristiohady, I. M. C. Binekada, La Ode Alifariki, L. Haya
Infection with Salmonella typhi bacteria during the COVID-19 era has the potential to worsen malnutrition in children in low- and middle-income nations, particularly around the coast. Recent studies have demonstrated that one of the best strategies for preventing malnutrition is consuming local food sources from the sea, which are easy to acquire, inexpensive, and high in nutrients. In this study, 15 male mice were used as the test subjects. They were split into two groups: the intervention group and the control group, both before and after intraperitoneal injection of Salmonella typhi. The intervention group was given dosages of cookies containing Diadema setosum gonad (0.40 mg/kg of body weight), whereas the control group received only a natural diet. The blood samples were then analyzed to measure their hemoglobin levels using the Sahli haemometer method, and serum albumin concentrations were determined using the bromocresol green method. The results show that the treatment with Diadema setosum gonad cookies had a significant effect on the levels of albumin and hemoglobin (Hb), as well as the body weights of the intervened mice, with a statistically significant increase (p<0.05). Conversely, BALB/c mice in the control group demonstrated a decrease in their initial food intakes, resulting in a significant reduction in body weight, albumin, and hemoglobin (Hb). This finding implies that the cookie formula with Diadema setosum gonad as the principal ingredient has potential benefits in lowering the incidence of malnutrition for children.
在2019冠状病毒病期间感染伤寒沙门氏菌有可能加剧中低收入国家,特别是沿海地区儿童的营养不良状况。最近的研究表明,预防营养不良的最佳策略之一是食用来自海洋的当地食物,这些食物容易获得,价格低廉,营养丰富。本研究以15只雄性小鼠为实验对象。将其分为干预组和对照组,分别在腹腔注射伤寒沙门菌前后注射。干预组给予含有绒水肿性腺的饼干(0.40 mg/kg体重),对照组只给予自然饮食。然后对血液样本进行分析,使用Sahli血液计法测量其血红蛋白水平,使用溴甲酚绿法测定血清白蛋白浓度。结果表明:硬水肿性腺饼干对干预小鼠的白蛋白、血红蛋白(Hb)水平及体重均有显著影响,差异均有统计学意义(p<0.05)。相反,对照组BALB/c小鼠的初始食物摄入量减少,导致体重、白蛋白和血红蛋白(Hb)显著减少。这一发现表明,以刚毛水肿性腺为主要成分的饼干配方在降低儿童营养不良发生率方面具有潜在的益处。
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引用次数: 0
Nutritional Strategies for Treating Iron Malnutrition: Implications on Nutrikinetic Approaches 治疗铁营养不良的营养策略:对营养动力学方法的影响
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.23
B. K. Chekraverthy, Harshita Arun Pardhe, A. R. Kiran, K. Nagappan
Malnutrition remains a major global issue-affecting people of all ages. Iron deficiency is one of the existing malnutrition deficiencies limiting long-term human development. Food-based interventions play a vital role in the growth, development (physical and cognitive), and socioeconomic status of an individual. Several food-based approaches (viz., fortification and bio fortification of staple foods) have been employed for improving malnutrition in vulnerable populations. In underdeveloped countries, staple foods like rice, wheat, beans, and pearl millets etc. are being actively fortified and bio fortified to enhance the iron content intended to deliver the required nourishment with optimal consumption. However, translational iron levels from functional foods may not be proportional to iron absorbed into systems. Considering the effectiveness of bioavailability, additional emphasis is required in establishing the same for the functional foods which could serve as an effective alternative to existing methods in mitigating iron malnutrition. This review emphasizes the importance of iron, iron physio-pathological, and the current status of food strategies in dealing with iron malnutrition.
营养不良仍然是一个重大的全球问题,影响着所有年龄段的人。缺铁是限制人类长期发展的现有营养不良缺陷之一。以食物为基础的干预措施在个人的生长、发育(身体和认知)和社会经济地位方面发挥着至关重要的作用。已经采用了几种以食物为基础的方法(即主食的强化和生物强化)来改善弱势群体的营养不良。在不发达国家,主食如大米、小麦、豆类和珍珠粟等正在积极强化和生物强化,以提高铁含量,以提供所需的营养和最佳的消费。然而,从功能性食品中摄取的铁含量可能与系统吸收的铁量不成比例。考虑到生物利用度的有效性,需要进一步强调在功能性食品中建立同样的生物利用度,这可以作为减轻铁营养不良的现有方法的有效替代方法。本文综述了铁的重要性,铁的生理病理,以及目前处理铁营养不良的食物策略的现状。
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引用次数: 1
A Bibliometric Framework for Quantifying Research on Kimchi, A Staple Korean Dish 韩国主食泡菜量化研究的文献计量学框架
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.4
Raghavendra G Raghavendra G, Nitish Domadhar, J. Mallya, Manish T. K
Kimchi is one of the fermented foods studied and discussed by researchers for many decades. Several perspectives have been considered while investigating the role of Kimchi. Thus, this demands a scientific review of this domain. This study is an honest attempt to map the scientific production of Kimchi. A descriptive, performance and bibliometric analysis were conducted to achieve the objective based on 924 documents recovered from the Scopus database. The results suggest a steady growth in the number of publications since 2016. The initial data shows that 160 journals have contributed 924 articles between 1979-2022. It is found that South Korea contributes nearly 94% (868 articles) of the total contribution, followed by the United States, China, and Japan. The institution-wise analysis (Figure 4) of the published articles based on the authors' affiliations suggests that most articles have been published by South Korean Institutes, followed by the United States. With 19 publications, the Journal of the Korean Society of Food Science and Nutrition has emerged as the most influential journal in Kimchi literature. The keyword analysis identifies nine themes. This analysis's findings reveal that the properties of Kimchi and the quality and sensory evaluation of kimchi were the most niche topics in this domain. Findings also revealed that the health benefits of Kimchi, the quality of Kimchi, and the bacteria involved in Kimchi fermentation were emerging themes in the Kimchi knowledge base. The findings of this study are helpful for future researchers in identifying the most influential authors, journals, and articles. Further, this study’s results also determine future research objectives in the Kimchi studies.
泡菜是研究人员研究和讨论了几十年的发酵食品之一。在调查泡菜的作用时,考虑了几个角度。因此,这需要对这一领域进行科学的审查。这项研究是对泡菜科学生产的诚实尝试。基于从Scopus数据库中恢复的924篇文献,进行了描述性、绩效和文献计量学分析。结果表明,自2016年以来,出版物数量稳步增长。初步数据显示,从1979年到2022年,160家期刊共发表了924篇文章。调查结果显示,韩国的论文占全部论文的94%(868篇),其次是美国、中国、日本。根据作者所属机构对已发表文章进行的机构分析(图4)表明,大多数文章是由韩国研究所发表的,其次是美国研究所。《韩国食品科学与营养学会杂志》共发表了19篇文章,是泡菜文献中最具影响力的杂志。关键词分析确定了九个主题。分析结果表明,泡菜的特性以及泡菜的质量和感官评价是该领域最热门的话题。研究结果还显示,泡菜的健康益处、泡菜的质量以及参与泡菜发酵的细菌是泡菜知识库中新兴的主题。本研究的发现有助于未来的研究人员识别最具影响力的作者、期刊和文章。此外,本研究的结果也确定了泡菜研究的未来研究目标。
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引用次数: 1
Effect of Health-Related Behavior and Nutrition Intake on the Blood Lipids Levels 健康相关行为和营养摄入对血脂水平的影响
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.15
Jooeun Lee
Preventing hyperlipidemia remains of critical importance as one of the main risk factors for cardiovascular disease, which is the leading cause of death every year. Therefore, the present study investigated the effect of health-related behavior and nutritional intake on blood lipid levels in adult men and women. Survey data from the 2019 National Health and Nutrition Examination conducted by the Korea Disease Control and Prevention Agency on 6,606 adults were analyzed to evaluate the effect of drinking, smoking, exercise, and nutrient intake on blood cholesterol, triglyceride, and HDL cholesterol. Blood total cholesterol showed significant differences in the mean based on the level of drinking (P < 0.05), and blood HDL-cholesterol and triglyceride showed significant differences in the mean levels of drinking, smoking, and exercise (P < 0.001). In regression analysis, blood total cholesterol was significantly affected by body mass index (BMI), education level, and menopause (P < 0.01, P < 0.001). Blood HDL-cholesterol was significantly affected by BMI, menopause, drinking, smoking, exercise, dietary intake of omega-3 fatty acids, and dietary cholesterol (P < 0.05, p < 0.01, P < 0.001), and blood triglyceride level was significantly affected by BMI, menopause, smoking, and exercise (P < 0.05, P < 0.001). Obesity, exercise, eating habits, smoking, and women's menopause were the main factors contributing to hyperlipidemia, so awareness of these factors should be raised.
作为心血管疾病的主要危险因素之一,预防高脂血症仍然至关重要,心血管疾病是每年死亡的主要原因。因此,本研究调查了健康相关行为和营养摄入对成年男性和女性血脂水平的影响。韩国疾病管理本部以6606名成年人为对象进行的“2019年国民健康营养调查”中,分析了饮酒、吸烟、运动、营养摄入对血液中胆固醇、甘油三酯、高密度脂蛋白胆固醇的影响。血液中总胆固醇在饮酒水平上的平均值有显著性差异(P < 0.05),血液中高密度脂蛋白胆固醇和甘油三酯在饮酒、吸烟和运动水平上的平均值有显著性差异(P < 0.001)。在回归分析中,血总胆固醇受身体质量指数(BMI)、文化程度、绝经期的影响显著(P < 0.01, P < 0.001)。BMI、绝经期、饮酒、吸烟、运动、膳食摄入omega-3脂肪酸、膳食胆固醇显著影响血hdl -胆固醇水平(P < 0.05, P < 0.01, P < 0.001), BMI、绝经期、吸烟、运动显著影响血甘油三酯水平(P < 0.05, P < 0.001)。肥胖、运动、饮食习惯、吸烟和女性更年期是导致高脂血症的主要因素,因此应该提高对这些因素的认识。
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引用次数: 0
Sappan Heartwood (Caesalpinia sappan L.) Extract as a Natural Antimicrobial used in Beetroot Juice by Accelerated Solvent Extraction 杉木(Caesalpinia Sappan L.)加速溶剂萃取法提取甜菜根汁中的天然抗菌剂
Pub Date : 2023-04-25 DOI: 10.12944/crnfsj.11.1.8
Rattana Muangrat, Yaowares Thipsuwan
After accelerated solvent extraction using ethanol-water solvent (at a weight ratio of 3 to 1) at extraction temperatures of 60C, 120C, and 180C for 5 min under 1500 psi extraction pressure, the brazilin content in the extracts was determined and also the obtained sappan heartwood extracts (SHE) were used to inhibit some pathogenic bacteria in food, including B. cereus, E. coli, S. aureus, and S. Typhimurium, using agar disc diffusion method. According to the findings of this study, the average yield of SHE using ethanol-water solvent at different extraction temperatures of 60C, 120C, and 180C was 9.16, 13.64 and 16.81%, respectively, providing that the brazilin compound was found in the extracts to be approximately 3.36, 2.69 and 2.68%, respectively. SHE samples were found to be antibacterial against all bacteria tested. These extracts' minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at 37°C for E. coli were 3.91 and 5.64-6.51 mg/ml, respectively; S. aureus, 3.69-3.91 and 3.69-3.91 mg/ml, respectively; B. cereus, 0.150.16 and 0.20 mg/ml, respectively, and S. Typhimurium, 0.96 and 1.31-1.96 mg/ml, respectively. SHE obtained at 120C extraction temperature were suitable and selected for addition into beetroot juice stored at 4C for 7 days and 37C for 24 hr, with the lowest SHE concentrations found to completely and simultaneously kill B. cereus, E. coli, S. aureus, and S. Typhimurium in beetroot juice being 11.73 and 3.91 mg/ml, respectively.
采用乙醇-水溶剂(重量比为3:1),在萃取温度为60C、120C和180C、萃取压力为1500 psi的条件下,加速萃取5 min,测定萃取物中巴西酮的含量,并利用琼脂盘扩散法对食品中蜡样芽孢杆菌、大肠杆菌、金黄色葡萄球菌和鼠伤寒杆菌等致病菌进行抑制。根据本研究的结果,在60C、120C和180C的不同提取温度下,乙醇-水溶剂SHE的平均得率分别为9.16%、13.64%和16.81%,这说明在提取物中发现的巴西林化合物分别约为3.36、2.69和2.68%。SHE样品被发现对所有细菌都有抗菌作用。37℃条件下对大肠杆菌的最低抑菌浓度(MIC)为3.91,最低杀菌浓度(MBC)为5.64 ~ 6.51 mg/ml;金黄色葡萄球菌分别为3.69 ~ 3.91和3.69 ~ 3.91 mg/ml;蜡样芽孢杆菌分别为0.15、0.16、0.20 mg/ml,鼠伤寒沙门氏菌分别为0.96、1.31 ~ 1.96 mg/ml。120C提取温度下得到的SHE适宜于加入4C保存7天、37C保存24小时的甜菜根汁中,能同时完全杀灭甜菜根汁中的蜡样芽孢杆菌、大肠杆菌、金黄色葡萄球菌和鼠伤寒沙门氏菌的SHE最低浓度分别为11.73和3.91 mg/ml。
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Current Research in Nutrition and Food Science Journal
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