首页 > 最新文献

DNA Sequence最新文献

英文 中文
Cloning of Rat Calcium-Modulating Cyclophilin Ligand 大鼠钙调节亲环蛋白配体的克隆
Pub Date : 2001-01-01 DOI: 10.3109/10425170109080777
Sung Joong Lee, Kathryn Drabik, E. Benveniste
Rat calcium-modulating cyclophilin ligand (CAML) cDNA was cloned and sequenced. It has a predicted open reading frame of 294 amino acids. The CAML gene is highly conserved throughout species, showing 85, 89 and 69% amino acid sequence identity to the human, mouse, and chicken genes, respectively. Gene expression data using astrocytes, microglia and neurons show that CAML mRNA and protein is constitu-tively expressed in these cell types of the central nervous system. The cloning of rat CAML will facilitate further investigations on the function of this molecule.
对大鼠钙调节亲环蛋白配体(CAML) cDNA进行了克隆和测序。它预计有294个氨基酸的开放阅读框。CAML基因在整个物种中高度保守,其氨基酸序列分别与人类、小鼠和鸡的85、89和69%相同。星形胶质细胞、小胶质细胞和神经元的基因表达数据显示,CAML mRNA和蛋白在中枢神经系统的这些细胞类型中组成性表达。大鼠CAML的克隆将有助于进一步研究其功能。
{"title":"Cloning of Rat Calcium-Modulating Cyclophilin Ligand","authors":"Sung Joong Lee, Kathryn Drabik, E. Benveniste","doi":"10.3109/10425170109080777","DOIUrl":"https://doi.org/10.3109/10425170109080777","url":null,"abstract":"Rat calcium-modulating cyclophilin ligand (CAML) cDNA was cloned and sequenced. It has a predicted open reading frame of 294 amino acids. The CAML gene is highly conserved throughout species, showing 85, 89 and 69% amino acid sequence identity to the human, mouse, and chicken genes, respectively. Gene expression data using astrocytes, microglia and neurons show that CAML mRNA and protein is constitu-tively expressed in these cell types of the central nervous system. The cloning of rat CAML will facilitate further investigations on the function of this molecule.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"88 1","pages":"209 - 213"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88230761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Analysis of the rpoN Locus in the Plant Pathogenic Bacterium, Pseudomonas Syringae pv. Glycinea 植物致病菌丁香假单胞菌rpoN位点的分析。Glycinea
Pub Date : 2001-01-01 DOI: 10.3109/10425170109042054
F. Alarcón-Chaidez, C. Bender
s`54, which is encoded by rpoN, is required for a variety of metabolic functions in bacteria including the utilization of alternative carbon and nitrogen sources, nitrogen fixation, and the expression of virulence determinants. Sequence analysis of a 3, 020-bp DNA fragment from the plant pathogen Pseudomonas syringae pv. glycinea PG4180 revealed four ORFs designated rpoN, orfA, orfB, and orfCδ, which were related to rpoN and rpoN-associated genes from other microorganisms. The rpoN upstream region in P. syringae contained two overlapping promoters, which may suggest a complex regulatory pattern. This is the first study describing the organization of the rpoN locus in P. syringae.
由rpoN编码的s ' 54在细菌的多种代谢功能中是必需的,包括利用替代碳和氮源、固氮和表达毒力决定因素。植物病原菌丁香假单胞菌3020bp DNA片段的序列分析。在glycinea PG4180中发现了4个orf,分别为rpoN、orfA、orfB和orfCδ,它们与来自其他微生物的rpoN和ron相关基因相关。丁香属rpoN上游区域包含两个重叠的启动子,可能存在复杂的调控模式。这是首次对丁香属rpoN位点的组织结构进行研究。
{"title":"Analysis of the rpoN Locus in the Plant Pathogenic Bacterium, Pseudomonas Syringae pv. Glycinea","authors":"F. Alarcón-Chaidez, C. Bender","doi":"10.3109/10425170109042054","DOIUrl":"https://doi.org/10.3109/10425170109042054","url":null,"abstract":"s`54, which is encoded by rpoN, is required for a variety of metabolic functions in bacteria including the utilization of alternative carbon and nitrogen sources, nitrogen fixation, and the expression of virulence determinants. Sequence analysis of a 3, 020-bp DNA fragment from the plant pathogen Pseudomonas syringae pv. glycinea PG4180 revealed four ORFs designated rpoN, orfA, orfB, and orfCδ, which were related to rpoN and rpoN-associated genes from other microorganisms. The rpoN upstream region in P. syringae contained two overlapping promoters, which may suggest a complex regulatory pattern. This is the first study describing the organization of the rpoN locus in P. syringae.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"88 1","pages":"77 - 84"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83811720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Isolation and Sequencing of Rhizobium Leguminosarum Bv. Trifolii PssN, PssO and PssP Genes Encoding the Proteins Involved in Polymerization and Translocation of Exopolysaccharide 豆科根瘤菌的分离与序列分析。三叶草psn、PssO和PssP基因编码参与外多糖聚合和易位的蛋白质
Pub Date : 2001-01-01 DOI: 10.3109/10425170109042046
A. Mazur, Jarostaw E. Król, A. Skorupska
Rhizobium leguminosarum bv. trifolii produces an acidic exopolysaccharide (EPS) that plays an important role in symbiotic interaction with clover plants. The sequence of 6.0-kb DNA fragment located upstream of the previously described prsDEorf3 and pssCDE genes involved in exopolysaccharide biosynthesis revealed three new genes designated pssN, pssO and pssP. The predicted protein product of pssP gene shares a significant homology to members of the membrane-peri-plasmic auxiliary (MPA1) family, that are involved in polymerization of the repeating subunits of EPS. The putative pssN protein product is highly homologous to the family of the outer membrane auxiliary (OMA) proteins engaged in translocation of polysaccharides in bacteria. The PssO did not reveal homology to the known bacterial proteins, but showed characteristic features of outer membrane proteins, and with PssN and PssP, it might be a part of the system involved in polymerization and translocation of EPS across the bacterial membranes.
豆科根瘤菌三叶草产生一种酸性外多糖(EPS),在与三叶草植物的共生作用中起重要作用。在先前描述的参与胞外多糖生物合成的prsDEorf3和pssCDE基因上游的6.0 kb DNA片段序列中,发现了三个新的基因,分别为psn、pssO和pssP。预测的pssP基因蛋白产物与膜-质周辅助(MPA1)家族成员具有显著的同源性,这些成员参与EPS重复亚基的聚合。推测的psn蛋白产物与参与细菌多糖易位的外膜辅助蛋白(OMA)家族高度同源。该PssO与已知细菌蛋白没有同源性,但具有外膜蛋白的特征,与psn和PssP可能是参与细菌膜上EPS聚合和易位的系统的一部分。
{"title":"Isolation and Sequencing of Rhizobium Leguminosarum Bv. Trifolii PssN, PssO and PssP Genes Encoding the Proteins Involved in Polymerization and Translocation of Exopolysaccharide","authors":"A. Mazur, Jarostaw E. Król, A. Skorupska","doi":"10.3109/10425170109042046","DOIUrl":"https://doi.org/10.3109/10425170109042046","url":null,"abstract":"Rhizobium leguminosarum bv. trifolii produces an acidic exopolysaccharide (EPS) that plays an important role in symbiotic interaction with clover plants. The sequence of 6.0-kb DNA fragment located upstream of the previously described prsDEorf3 and pssCDE genes involved in exopolysaccharide biosynthesis revealed three new genes designated pssN, pssO and pssP. The predicted protein product of pssP gene shares a significant homology to members of the membrane-peri-plasmic auxiliary (MPA1) family, that are involved in polymerization of the repeating subunits of EPS. The putative pssN protein product is highly homologous to the family of the outer membrane auxiliary (OMA) proteins engaged in translocation of polysaccharides in bacteria. The PssO did not reveal homology to the known bacterial proteins, but showed characteristic features of outer membrane proteins, and with PssN and PssP, it might be a part of the system involved in polymerization and translocation of EPS across the bacterial membranes.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"1 - 12"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89401906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Cloning of the Proto-oncogene c-src from Rat Testis 大鼠睾丸原癌基因c-src的克隆
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084469
Otor Al-Khalilt, B. Duke, S. Zeltwanger, D. Eaton, B. Spier, J. Stockand
The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.
利用高强度聚合酶链反应从大鼠睾丸中克隆出癌基因v-src的细胞同源物,即原癌基因c-src。大鼠c-src cDNA分别与鸡和小鼠、劳斯肉瘤病毒c-src和v-src同源。大鼠c-Src蛋白与人和小鼠c-Src蛋白同源性均达98%。有趣的是,与小鼠的蛋白质相比,大鼠的Src含有一个额外的氨基酸。正如预期的那样,大鼠睾丸Src缺乏与人类和小鼠中发现的神经元Src共同的6个额外残基。报告非神经元大鼠c-src的cDNA序列,将有助于大鼠组织作为人类疾病模型的细胞生长和转化实验。
{"title":"Cloning of the Proto-oncogene c-src from Rat Testis","authors":"Otor Al-Khalilt, B. Duke, S. Zeltwanger, D. Eaton, B. Spier, J. Stockand","doi":"10.3109/10425170109084469","DOIUrl":"https://doi.org/10.3109/10425170109084469","url":null,"abstract":"The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"80 1","pages":"425 - 429"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75618768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Short Communication: Exon/Intron Organisation of Human Proteasome PROS-27 K Gene 短通讯:人蛋白酶体pro - 27k基因的外显子/内含子组织
Pub Date : 2001-01-01 DOI: 10.3109/10425170109025000
T. Sjakste, N. Sjakste, K. Scherrer
The exon/intron structure of the human proteasome PROS-27 gene was established by means of partial sequencing of its genomic clones and comparison with the chromosome 14 sequences from the data bases. The gene contains seven exons spanning over 19 kb. Introns of the gene contain numerous Alu type repeats, Mer 2 and LINE type repeats. Pattern of the repeats indicates conservatism of the sequence.
通过对人蛋白酶体PROS-27基因组克隆进行部分测序,并与数据库中的14号染色体序列进行比对,建立了PROS-27基因的外显子/内含子结构。该基因包含7个外显子,长度超过19kb。该基因内含子含有大量的Alu型重复序列、Mer 2型重复序列和LINE型重复序列。重复序列的模式表明该序列的保守性。
{"title":"Short Communication: Exon/Intron Organisation of Human Proteasome PROS-27 K Gene","authors":"T. Sjakste, N. Sjakste, K. Scherrer","doi":"10.3109/10425170109025000","DOIUrl":"https://doi.org/10.3109/10425170109025000","url":null,"abstract":"The exon/intron structure of the human proteasome PROS-27 gene was established by means of partial sequencing of its genomic clones and comparison with the chromosome 14 sequences from the data bases. The gene contains seven exons spanning over 19 kb. Introns of the gene contain numerous Alu type repeats, Mer 2 and LINE type repeats. Pattern of the repeats indicates conservatism of the sequence.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"12 1","pages":"261 - 265"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81834218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Cloning and Expression Analysis of Two Cotton (Gossypium Hirsutum L.) Genes Encoding Cell Wall Proline-rich Proteins 两种棉花(Gossypium Hirsutum L.)的克隆与表达分析编码细胞壁脯氨酸丰富蛋白的基因
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084461
H. Tan, R. G. Creech, J. Jenkins, Yungfu Chang, D. Ma
Two cotton (Gossypium hirsutum L.) genes, ghprpl and ghprpl, encoding cell wall proline-rich proteins (PRPs) have been cloned and characterized. The ghprpl gene has an open reading frame (ORF) that encodes a PRP of 299 amino acids (aa), whereas the ghprp2 gene contains an ORF that codes for a 310-aa PRP. The GhPRPl has an 80% identity in aa sequence with that of GhPRP2. Like other plant cell wall PRPs, both cotton PRPs have a hydrophobic signal peptide at their N-termini, followed by repeating peptide units. Northern blot analyses showed that the ghprp1 gene is predominantly expressed in the fiber during the elongation stage of fiber development. Reverse transcription (RT)-PCR analysis showed that ghprp1 is expressed in both fiber and root tissues, whereas ghprpl is in roots only. The ghprp1 gene was shown to be present in the A1, A2, D1 and D5 genomes of Gossypium by PCR amplification, whereas the ghprpl gene is only present in the A1 and A2 genomes. The ghprp1 gene was over-expressed in the yeast Pichia pastoris, and the expressed GhPRP1 protein was used as an antigen to raise polyclonal antibodies (anti-GhPRP1). Western analysis using the anti-GhPRP1 probe detected a major protein band (50 kDa) in 5-31-day postanthesis (DPA) fibers. However, the 50 kDa protein was absent in other cotton tissues.
克隆并鉴定了棉花细胞壁富含脯氨酸蛋白(PRPs)的两个基因ghprpl和ghprpl。ghprpl基因有一个开放阅读框(ORF),编码299个氨基酸(aa)的PRP,而ghprp2基因包含一个开放阅读框(ORF),编码310-aa的PRP。GhPRPl与GhPRP2在a序列上有80%的同源性。与其他植物细胞壁PRPs一样,两种棉花PRPs在其n端都有一个疏水信号肽,随后是重复的肽单元。Northern blot分析表明,ghprp1基因在纤维发育的伸长阶段主要在纤维中表达。反转录(RT)-PCR分析表明,ghprp1在纤维和根组织中均有表达,而ghprpl仅在根中表达。PCR扩增结果显示,ghprp1基因存在于棉的A1、A2、D1和D5基因组中,而ghprpl基因仅存在于棉的A1和A2基因组中。将ghprp1基因在酵母毕赤酵母中过表达,将表达的ghprp1蛋白作为抗原,培养多克隆抗体(抗ghprp1)。Western分析使用抗ghprp1探针在5-31天的DPA纤维中检测到一个主要的蛋白带(50 kDa)。而在棉花的其他组织中不存在50 kDa蛋白。
{"title":"Cloning and Expression Analysis of Two Cotton (Gossypium Hirsutum L.) Genes Encoding Cell Wall Proline-rich Proteins","authors":"H. Tan, R. G. Creech, J. Jenkins, Yungfu Chang, D. Ma","doi":"10.3109/10425170109084461","DOIUrl":"https://doi.org/10.3109/10425170109084461","url":null,"abstract":"Two cotton (Gossypium hirsutum L.) genes, ghprpl and ghprpl, encoding cell wall proline-rich proteins (PRPs) have been cloned and characterized. The ghprpl gene has an open reading frame (ORF) that encodes a PRP of 299 amino acids (aa), whereas the ghprp2 gene contains an ORF that codes for a 310-aa PRP. The GhPRPl has an 80% identity in aa sequence with that of GhPRP2. Like other plant cell wall PRPs, both cotton PRPs have a hydrophobic signal peptide at their N-termini, followed by repeating peptide units. Northern blot analyses showed that the ghprp1 gene is predominantly expressed in the fiber during the elongation stage of fiber development. Reverse transcription (RT)-PCR analysis showed that ghprp1 is expressed in both fiber and root tissues, whereas ghprpl is in roots only. The ghprp1 gene was shown to be present in the A1, A2, D1 and D5 genomes of Gossypium by PCR amplification, whereas the ghprpl gene is only present in the A1 and A2 genomes. The ghprp1 gene was over-expressed in the yeast Pichia pastoris, and the expressed GhPRP1 protein was used as an antigen to raise polyclonal antibodies (anti-GhPRP1). Western analysis using the anti-GhPRP1 probe detected a major protein band (50 kDa) in 5-31-day postanthesis (DPA) fibers. However, the 50 kDa protein was absent in other cotton tissues.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"22 1","pages":"367 - 380"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89359590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Sequence Analysis of the Rat Phenylalanine Hydroxylase Gene Promoter 大鼠苯丙氨酸羟化酶基因启动子序列分析
Pub Date : 2001-01-01 DOI: 10.3109/10425170109080774
Diane Rees, M. J. Fisher, I. McDowall
We have characterized the 5′-end (3218bp) of the rat phenylalanine hydroxylase (PAH) gene. Within this PAH promoter sequence, we have identified a number of putative regulatory sites analogous to those present in the human and murine PAH promoters. In particular, potential HNF 1 binding sites and a CRE have been identified. These sequences respectively bind HNF1 and CREB transcription factors present in rat nuclear extracts and may be significant in the tissue-specific and hormonal control of PAH expression.
我们已经鉴定了大鼠苯丙氨酸羟化酶(PAH)基因的5 '端(3218bp)。在这个多环芳烃启动子序列中,我们已经确定了一些类似于人类和小鼠多环芳烃启动子中存在的假定调控位点。特别是,已经确定了潜在的hnf1结合位点和CRE。这些序列分别结合大鼠核提取物中存在的HNF1和CREB转录因子,可能在PAH表达的组织特异性和激素控制中具有重要意义。
{"title":"Sequence Analysis of the Rat Phenylalanine Hydroxylase Gene Promoter","authors":"Diane Rees, M. J. Fisher, I. McDowall","doi":"10.3109/10425170109080774","DOIUrl":"https://doi.org/10.3109/10425170109080774","url":null,"abstract":"We have characterized the 5′-end (3218bp) of the rat phenylalanine hydroxylase (PAH) gene. Within this PAH promoter sequence, we have identified a number of putative regulatory sites analogous to those present in the human and murine PAH promoters. In particular, potential HNF 1 binding sites and a CRE have been identified. These sequences respectively bind HNF1 and CREB transcription factors present in rat nuclear extracts and may be significant in the tissue-specific and hormonal control of PAH expression.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"191 - 195"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86543084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Human Membrane Progesterone Receptor Gene: Genomic Structure and Promoter Analysis 人膜孕酮受体基因:基因组结构和启动子分析
Pub Date : 2001-01-01 DOI: 10.3109/10425170109042047
Sabine Bernauer, M. Wehling, Dirk Gerdes And, E. Falkenstein
Rapid, nongenomic effects of steroids are likely to be mediated by membrane receptors not by intracellular steroid receptors. We recently identified a progesterone membrane binding protein (mPR) from human liver. The corresponding hmpr gene is comprised of 3 exons and 2 introns. The promoter sequence of hmpr lacks a typical TATA box but contains instead a high homology to a transcription Initiatior consensus sequence, which overlaps the experimentally determined transcriptional start site. The major proximal promoter is GC-rich and sequence analysis revealed a CpG island spanning the transcriptional start site. Several putative cis-regulatory DNA-motifs, which represent possible binding sites for transcription factors like AP2, NF-AT, Ahr/Arnt and C/EBP were identified in the genomic upstream region by sequence homology. Functional analysis of differently deleted fragments of the hmpr upstream region in a GFP-reportergene assay in transiently transfected cultured cells indicates the general testability of the hmpr promoter in vivo.
类固醇的快速非基因组效应可能是由膜受体介导的,而不是由细胞内类固醇受体介导的。我们最近从人肝脏中鉴定出一种黄体酮膜结合蛋白(mPR)。相应的hmpr基因由3个外显子和2个内含子组成。hmpr的启动子序列缺乏典型的TATA盒,但包含与转录起始一致序列的高度同源性,该序列与实验确定的转录起始位点重叠。主要的近端启动子富含gc,序列分析显示一个CpG岛横跨转录起始位点。通过序列同源性,在基因组上游区域鉴定了几个推测的顺式调控dna基序,它们代表了AP2、NF-AT、Ahr/Arnt和C/EBP等转录因子的可能结合位点。在瞬时转染的培养细胞中,gfp报告基因实验对hmpr上游区域不同缺失片段的功能分析表明,hmpr启动子在体内具有一般的可测试性。
{"title":"The Human Membrane Progesterone Receptor Gene: Genomic Structure and Promoter Analysis","authors":"Sabine Bernauer, M. Wehling, Dirk Gerdes And, E. Falkenstein","doi":"10.3109/10425170109042047","DOIUrl":"https://doi.org/10.3109/10425170109042047","url":null,"abstract":"Rapid, nongenomic effects of steroids are likely to be mediated by membrane receptors not by intracellular steroid receptors. We recently identified a progesterone membrane binding protein (mPR) from human liver. The corresponding hmpr gene is comprised of 3 exons and 2 introns. The promoter sequence of hmpr lacks a typical TATA box but contains instead a high homology to a transcription Initiatior consensus sequence, which overlaps the experimentally determined transcriptional start site. The major proximal promoter is GC-rich and sequence analysis revealed a CpG island spanning the transcriptional start site. Several putative cis-regulatory DNA-motifs, which represent possible binding sites for transcription factors like AP2, NF-AT, Ahr/Arnt and C/EBP were identified in the genomic upstream region by sequence homology. Functional analysis of differently deleted fragments of the hmpr upstream region in a GFP-reportergene assay in transiently transfected cultured cells indicates the general testability of the hmpr promoter in vivo.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"111 1","pages":"13 - 25"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85795293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Identification and Sequence Determination of mRNAs Detected in Dormant (Diapausing) Aedes triseriatus Mosquito Embryos 休眠(滞育)三体伊蚊胚胎mrna的鉴定与序列测定
Pub Date : 2001-01-01 DOI: 10.3109/10425170109080775
B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty
Many insects survive adverse climatic conditions in a dormant state known as diapause. In this study, we identified and sequenced several mRNAs in diapausing Aedes triseriatus mosquito embryos. Using reverse-transcription PCR and 5′ RACE, we identified a 995-nucleotide cDNA that encodes a 259-amino acid protein of unknown function. This putative protein displays strong sequence similarity to Drosophila mela-nogaster (95%), human (87%), Caenorhabditis elegans (86%) and yeast (81%) counterparts. The second identified full-length cDNA consists of 624 nucleotides and encodes a 174-amino acid protein of unknown function. This putative protein displays significant sequence similarity to D. melanogaster (68%), human (59%), plant (57%) and yeast (49%) counterparts. We also detected a number of cDNA fragments that exhibited significant sequence similarity to a mitochondrial cytochrome C oxidase subunit, human N33 protein (a potential human prostate tumor suppressor), 18S and 28S ribosomal RNAs, protein disulfide-isomerase, and guanine nucleotide-binding protein.
许多昆虫在被称为滞育的休眠状态下在恶劣的气候条件下存活下来。在本研究中,我们鉴定并测序了滞育三体伊蚊胚胎中的几个mrna。利用反转录PCR和5 ' RACE技术,我们鉴定出一个995个核苷酸的cDNA,编码一个功能未知的259个氨基酸的蛋白质。该推测的蛋白序列与果蝇(95%)、人类(87%)、秀丽隐杆线虫(86%)和酵母(81%)具有很强的相似性。第二个全长cDNA由624个核苷酸组成,编码一个174个氨基酸的功能未知的蛋白质。该推测的蛋白与黑胃菌(68%)、人类(59%)、植物(57%)和酵母(49%)的对应蛋白具有显著的序列相似性。我们还检测到许多cDNA片段与线粒体细胞色素C氧化酶亚基、人类N33蛋白(一种潜在的人类前列腺肿瘤抑制因子)、18S和28S核糖体rna、蛋白二硫异构酶和鸟嘌呤核苷酸结合蛋白具有显著的序列相似性。
{"title":"Identification and Sequence Determination of mRNAs Detected in Dormant (Diapausing) Aedes triseriatus Mosquito Embryos","authors":"B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty","doi":"10.3109/10425170109080775","DOIUrl":"https://doi.org/10.3109/10425170109080775","url":null,"abstract":"Many insects survive adverse climatic conditions in a dormant state known as diapause. In this study, we identified and sequenced several mRNAs in diapausing Aedes triseriatus mosquito embryos. Using reverse-transcription PCR and 5′ RACE, we identified a 995-nucleotide cDNA that encodes a 259-amino acid protein of unknown function. This putative protein displays strong sequence similarity to Drosophila mela-nogaster (95%), human (87%), Caenorhabditis elegans (86%) and yeast (81%) counterparts. The second identified full-length cDNA consists of 624 nucleotides and encodes a 174-amino acid protein of unknown function. This putative protein displays significant sequence similarity to D. melanogaster (68%), human (59%), plant (57%) and yeast (49%) counterparts. We also detected a number of cDNA fragments that exhibited significant sequence similarity to a mitochondrial cytochrome C oxidase subunit, human N33 protein (a potential human prostate tumor suppressor), 18S and 28S ribosomal RNAs, protein disulfide-isomerase, and guanine nucleotide-binding protein.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"54 1","pages":"197 - 202"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73526789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Genomic Organisation of the Mouse Ret Proto-Oncogene 小鼠Ret原癌基因的基因组组织
Pub Date : 2001-01-01 DOI: 10.3109/10425170109041333
D. Panetta, L. Yin, R. Barale, G. Romeo, R. Ravazzolo, Isabella Ceccherinp, A. Pulit
The RET proto-oncogene is involved in the development of both kidney and neural crests derived tissues. RET deleterious mutations cause hereditary neuroendocrine tumours and congenital intestinal agangliono-sis. Ongoing efforts aimed at elucidating the function of this gene include expression studies in different species and in transgenic mice. As first step in the study of Ret expression in mouse, we obtained the mouse Ret genomic structure. Intron-exon boundaries were determined and sequenced, all introns but the first one were amplified and cloned, and exons positioned in a restriction map. Mouse and human genes comparison indicates a highly conserved genomic organisation, except for exon 21 which is not conserved in mouse. A region extending 386 bp 5′ to the first exon was sequenced and compared with its human counterpart. Some features, reported for the human promoter, like the absence of TATA or CAAT boxes and a high GC content, are conserved.
RET原癌基因参与肾脏和神经嵴衍生组织的发育。RET有害突变可引起遗传性神经内分泌肿瘤和先天性肠神经节病。正在进行的旨在阐明该基因功能的工作包括在不同物种和转基因小鼠中的表达研究。作为小鼠Ret表达研究的第一步,我们获得了小鼠Ret基因组结构。确定内含子和外显子的边界并测序,扩增和克隆除第一个内含子外的所有内含子,并将外显子定位在限制子图中。小鼠与人类基因比较表明,除外显子21在小鼠中不保守外,其他基因组组织高度保守。测序了一个延伸至第一个外显子386 bp 5 '的区域,并与人类的对应区域进行了比较。据报道,人类启动子的一些特征,如没有TATA或CAAT盒和高GC含量,是保守的。
{"title":"Genomic Organisation of the Mouse Ret Proto-Oncogene","authors":"D. Panetta, L. Yin, R. Barale, G. Romeo, R. Ravazzolo, Isabella Ceccherinp, A. Pulit","doi":"10.3109/10425170109041333","DOIUrl":"https://doi.org/10.3109/10425170109041333","url":null,"abstract":"The RET proto-oncogene is involved in the development of both kidney and neural crests derived tissues. RET deleterious mutations cause hereditary neuroendocrine tumours and congenital intestinal agangliono-sis. Ongoing efforts aimed at elucidating the function of this gene include expression studies in different species and in transgenic mice. As first step in the study of Ret expression in mouse, we obtained the mouse Ret genomic structure. Intron-exon boundaries were determined and sequenced, all introns but the first one were amplified and cloned, and exons positioned in a restriction map. Mouse and human genes comparison indicates a highly conserved genomic organisation, except for exon 21 which is not conserved in mouse. A region extending 386 bp 5′ to the first exon was sequenced and compared with its human counterpart. Some features, reported for the human promoter, like the absence of TATA or CAAT boxes and a high GC content, are conserved.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"69 1","pages":"501 - 506"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89105071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
期刊
DNA Sequence
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1