Pub Date : 2001-01-01DOI: 10.3109/10425170109047568
V. H. Liao, J. Freedman
A homologue of pyruvate carboxylase was isolated as an expressed sequence tag during the identification of cadmium-responsive genes in Caenorhabditis elegans. The C. elegans protein, designated PYC-1, is predicted to contain 1,175 amino acids with a molecular mass of 129,284. Amino acid sequence analysis indicates that PYC-1 will be translocated into mitochondria. PYC-1 has high levels of amino acid sequence identity with other pyruvate carboxylases. The highest levels of identity are in the putative transcarboxylation, biotin carboxylation and biotin carboxyl carrier domains.
{"title":"Characterization of a Cadmium-Inducible Isoform of Pyruvate Carboxylase from Caenorhabditis elegans","authors":"V. H. Liao, J. Freedman","doi":"10.3109/10425170109047568","DOIUrl":"https://doi.org/10.3109/10425170109047568","url":null,"abstract":"A homologue of pyruvate carboxylase was isolated as an expressed sequence tag during the identification of cadmium-responsive genes in Caenorhabditis elegans. The C. elegans protein, designated PYC-1, is predicted to contain 1,175 amino acids with a molecular mass of 129,284. Amino acid sequence analysis indicates that PYC-1 will be translocated into mitochondria. PYC-1 has high levels of amino acid sequence identity with other pyruvate carboxylases. The highest levels of identity are in the putative transcarboxylation, biotin carboxylation and biotin carboxyl carrier domains.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"82 1","pages":"137 - 145"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72712961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109025001
S. Dühr, A. Torres-Montaner, A. Astola, F. García-Cózar, C. Pendón, J. Bolívar, M. Valdivia
Upstream binding factor, UBF, is a nucleolar autoantigen involved in the transcription of ribosomal DNA genes. Previously, human genomic clones served to demonstrate that an alternative pre-mRNA splicing of a single gene is used to form UBF1 and UBF2. Here, to complete characterizing the 5′end genomic organization of this nucleolar transcription factor, lambda clones containing the human UBF gene were isolated from a human placenta genomic library using a hamster UBF cDNA as a probe. An additional PCR product was isolated from HeLa genomic DNA to cover the first translated 60 nt of the gene containing the ATG initiation codon. We have also determined the transcription start site of the gene by primer extension analysis at nt 188 upstream from the start ATG codon. It served first, to identify an untranslated initial exon on the UBF gene covering the first 121 nt of human UBF cDNA, and also to establish the sequence of the proximal promoter. The human UBF promoter lacks a TATA and CAAT boxes but contains multiple binding sites for SP1, API, AP2, TFIID, NF-1 and a single site for NFAT-1. Consequently we have defined the first five exons of the human UBF gene covering 7.5 kb. The complete gene now consists of 20 exons with intervening sequences and spans approximately 15kb of DNA.
{"title":"Short Communication: Molecular Analysis of the 5′ Region of Human Ribosomal Transcription Factor UBF","authors":"S. Dühr, A. Torres-Montaner, A. Astola, F. García-Cózar, C. Pendón, J. Bolívar, M. Valdivia","doi":"10.3109/10425170109025001","DOIUrl":"https://doi.org/10.3109/10425170109025001","url":null,"abstract":"Upstream binding factor, UBF, is a nucleolar autoantigen involved in the transcription of ribosomal DNA genes. Previously, human genomic clones served to demonstrate that an alternative pre-mRNA splicing of a single gene is used to form UBF1 and UBF2. Here, to complete characterizing the 5′end genomic organization of this nucleolar transcription factor, lambda clones containing the human UBF gene were isolated from a human placenta genomic library using a hamster UBF cDNA as a probe. An additional PCR product was isolated from HeLa genomic DNA to cover the first translated 60 nt of the gene containing the ATG initiation codon. We have also determined the transcription start site of the gene by primer extension analysis at nt 188 upstream from the start ATG codon. It served first, to identify an untranslated initial exon on the UBF gene covering the first 121 nt of human UBF cDNA, and also to establish the sequence of the proximal promoter. The human UBF promoter lacks a TATA and CAAT boxes but contains multiple binding sites for SP1, API, AP2, TFIID, NF-1 and a single site for NFAT-1. Consequently we have defined the first five exons of the human UBF gene covering 7.5 kb. The complete gene now consists of 20 exons with intervening sequences and spans approximately 15kb of DNA.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"23 1","pages":"267 - 272"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81643622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109042053
Jae-Seong Lee, M. Gye
Intron 2 of the β-actin genes from several fish species was sequenced and aligned with a Clustal W program for maximum similarity. From the maximum similarity and phylip data, it was noted that the intron 2 sequences would be useful for establishing a molecular phylogenetic tree and in elucidating the gene flow through evolution, especially at the family level.
{"title":"Use of β-Actin Gene Intron 2 as a Phylogenetic Marker in Fish Taxonomy","authors":"Jae-Seong Lee, M. Gye","doi":"10.3109/10425170109042053","DOIUrl":"https://doi.org/10.3109/10425170109042053","url":null,"abstract":"Intron 2 of the β-actin genes from several fish species was sequenced and aligned with a Clustal W program for maximum similarity. From the maximum similarity and phylip data, it was noted that the intron 2 sequences would be useful for establishing a molecular phylogenetic tree and in elucidating the gene flow through evolution, especially at the family level.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"10 1","pages":"71 - 76"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81982362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041332
A. A. Pedersen, N. Videbaek, K. Skak, H. V. Petersen, B. Michelsen
GAD65 and GAD67 are two isoforms of the enzyme glutamic acid decarboxylase which catalyze the production of GABA from glutamate, primarily in the brain. However, GAD and GABA also prevail in the retina, testes and islets of Langerhans. The main function of GABA is in neurotransmission, and it is involved in paracrine signalling in islets, but has also been suggested to play a role as a trophic factor in syn-aptogenesis and to be an important metabolite feeding into the tricarboxylic acid cycle via the GABA-shunt. Both GAD isoforms are subject to regulation, e.g. by synaptic activity. GAD65 is regulated at the level of enzyme activity by association and dissociation from its cofactor, PLP, whereas GAD67 is controlled at the level of its mRNA. To study this process in further detail, we have isolated and characterized the 5′-flank-ing region of the rat GAD67 gene. We report the transcriptional initiation sites and promoter sequences important for expression in islet P-cells and C6 glioma cells, and demonstrate that the GAD67 promoter harbors elements that are responsive to glucose in primary islet cells.
{"title":"Characterization of the Rat Gad67 Gene Promoter Reveals Elements Important for Basal Transcription and Glucose Responsiveness","authors":"A. A. Pedersen, N. Videbaek, K. Skak, H. V. Petersen, B. Michelsen","doi":"10.3109/10425170109041332","DOIUrl":"https://doi.org/10.3109/10425170109041332","url":null,"abstract":"GAD65 and GAD67 are two isoforms of the enzyme glutamic acid decarboxylase which catalyze the production of GABA from glutamate, primarily in the brain. However, GAD and GABA also prevail in the retina, testes and islets of Langerhans. The main function of GABA is in neurotransmission, and it is involved in paracrine signalling in islets, but has also been suggested to play a role as a trophic factor in syn-aptogenesis and to be an important metabolite feeding into the tricarboxylic acid cycle via the GABA-shunt. Both GAD isoforms are subject to regulation, e.g. by synaptic activity. GAD65 is regulated at the level of enzyme activity by association and dissociation from its cofactor, PLP, whereas GAD67 is controlled at the level of its mRNA. To study this process in further detail, we have isolated and characterized the 5′-flank-ing region of the rat GAD67 gene. We report the transcriptional initiation sites and promoter sequences important for expression in islet P-cells and C6 glioma cells, and demonstrate that the GAD67 promoter harbors elements that are responsive to glucose in primary islet cells.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"76 1","pages":"485 - 499"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87131686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109080770
H. Mayer, Martina Pongratz, R. Prohaska
We identified and characterized the cDNA coding for human LANCL2, a new member of the eukaryotic LanC-like protein family which is related to the bacterial lanthionine synthetase components C (LanC). The composite nucleotide sequence revealed a coding region of 1353 bp, a 5′-UTR of 186 bp and a 3'-UTR of 2421 bp. The deduced sequence of 450 amino acids showed 57.9 % identity (74.7 % similarity) when compared with the human LANCL1 homologue. In contrast to LANCL1, a unique ATP/GTP-binding site motif A was found in LANCL2. Northern blot analysis revealed the presence of two major transcripts in the brain, 4.7 kb and 4.1 kb in size, and a major 1.8 kb transcript in testis. Accordingly, expression array analysis showed prominent signals in these tissues. Because of the structural similarity to LanC, we postulate that LANCL2 may play a role as a component of a pep-tide-modifying complex.
{"title":"Molecular Cloning, Characterization, and Tissue-Specific Expression of Human LANCL2, a Novel Member of the LanC-Like Protein Family","authors":"H. Mayer, Martina Pongratz, R. Prohaska","doi":"10.3109/10425170109080770","DOIUrl":"https://doi.org/10.3109/10425170109080770","url":null,"abstract":"We identified and characterized the cDNA coding for human LANCL2, a new member of the eukaryotic LanC-like protein family which is related to the bacterial lanthionine synthetase components C (LanC). The composite nucleotide sequence revealed a coding region of 1353 bp, a 5′-UTR of 186 bp and a 3'-UTR of 2421 bp. The deduced sequence of 450 amino acids showed 57.9 % identity (74.7 % similarity) when compared with the human LANCL1 homologue. In contrast to LANCL1, a unique ATP/GTP-binding site motif A was found in LANCL2. Northern blot analysis revealed the presence of two major transcripts in the brain, 4.7 kb and 4.1 kb in size, and a major 1.8 kb transcript in testis. Accordingly, expression array analysis showed prominent signals in these tissues. Because of the structural similarity to LanC, we postulate that LANCL2 may play a role as a component of a pep-tide-modifying complex.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"110 1","pages":"161 - 166"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87684571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084475
L. Stamm, H. Bergen
A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.
对齿状密螺旋体4.2 kb DNA区包含4个完整基因(orf1、fliQ、fliR和flhB)和一个截断基因(flhA1)进行测序和分析。flq、FliR、FlhB和FlhA'的氨基酸序列与鞭毛输出器相关的细菌蛋白具有显著的同源性，而Orfl的氨基酸序列与大肠杆菌乙醇脱氢酶具有显著的同源性。在fliQ上游发现了一个推测的α70样启动子。RT-PCR分析表明，fliQ、fliR、flhB和flhA1独立于orfl共转录，提示运动相关基因是一个操纵子的组成部分。denticola T. fliQ-flhA1基因与对应的T. pallidum和Borrelia burgdorferi基因的位置不同，后者分别存在于大鞭毛操纵子或flgB鞭毛操纵子中。
{"title":"Molecular Characterization of the Treponema denticola fliQ Region","authors":"L. Stamm, H. Bergen","doi":"10.3109/10425170109084475","DOIUrl":"https://doi.org/10.3109/10425170109084475","url":null,"abstract":"A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"2 1","pages":"463 - 467"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88972969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109047561
Laura Carim-Todd, L. Sumoy, N. Andreu, X. Estivill, M. Escarceller
We have identified C15orf4, a novel human gene showing homology to the yeast mitochondrial ribosomal protein YmL30. C15orf4 encodes a transcript of 1,006 nt with an ORF of 279 amino acids and a predicted protein size of 31.7 kDa. Expression of C15orf4 is enriched in testis. C15orf4 was positioned to chromosome 15q24 by radiation hybrid mapping. We have identified the C15orf4 mouse orthologue as well as homologues in other species.
{"title":"Cloning, Mapping and Expression Analysis of C15 or f4, a Novel Human Gene with Homology to the Yeast Mitochondrial Ribosomal Protein Yml30 Gene","authors":"Laura Carim-Todd, L. Sumoy, N. Andreu, X. Estivill, M. Escarceller","doi":"10.3109/10425170109047561","DOIUrl":"https://doi.org/10.3109/10425170109047561","url":null,"abstract":"We have identified C15orf4, a novel human gene showing homology to the yeast mitochondrial ribosomal protein YmL30. C15orf4 encodes a transcript of 1,006 nt with an ORF of 279 amino acids and a predicted protein size of 31.7 kDa. Expression of C15orf4 is enriched in testis. C15orf4 was positioned to chromosome 15q24 by radiation hybrid mapping. We have identified the C15orf4 mouse orthologue as well as homologues in other species.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"24 1","pages":"91 - 96"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78415378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109024997
Gabriela Silva, C. Rodrigues-Pousada
The nucleotide sequence of a 6940 bp DNA fragment from Desulfovibrio gigas, containing seven ORFs was determined. ORF-1 encodes a probable lipoprotein having high similarities with lytic transglycosylases. ORF-2 encodes a polypeptide that does not show homologies with proteins deposited in the database, but it contains the consensus pattern of class II aminoacyl-tRNA synthetases. The putative protein encoded by ORF-3 possesses high similarities with universal stress response proteins from the UspA family. Northern blot analysis of ORF-3 shows that it is constitutively and abundantly expressed. ORF-4 encodes a probable helix-turn-helix-containing DNA-binding protein, given the presence of the helix-turn-helix motif, characteristic of this class of proteins. Its N-terminal region has high identity with its counterparts from proteins belonging to the RRF2 family. Northern blot analysis shows that ORF-4 is transcribed as a single mRNA in contrast to its orthologue from Desulfovibrio vulgaris. ORF-5 encodes a putative fusion protein as its N- and C-termini show significant homologies with molybdenum formylmethanofuran dehydrogenase and molybdopterin biosynthesis proteins, respectively. ORF-7 encodes a prokaryotic lipoprotein having homologies with multidrug efflux and DNA damage-inducible proteins, and it is constitutively and abundantly expressed.
{"title":"A 6940 bp DNA Fragment from Desulfovibrio gigas Contains Genes Coding for Lipoproteins, Universal Stress Response and Transcriptional Regulator Protein Homologues","authors":"Gabriela Silva, C. Rodrigues-Pousada","doi":"10.3109/10425170109024997","DOIUrl":"https://doi.org/10.3109/10425170109024997","url":null,"abstract":"The nucleotide sequence of a 6940 bp DNA fragment from Desulfovibrio gigas, containing seven ORFs was determined. ORF-1 encodes a probable lipoprotein having high similarities with lytic transglycosylases. ORF-2 encodes a polypeptide that does not show homologies with proteins deposited in the database, but it contains the consensus pattern of class II aminoacyl-tRNA synthetases. The putative protein encoded by ORF-3 possesses high similarities with universal stress response proteins from the UspA family. Northern blot analysis of ORF-3 shows that it is constitutively and abundantly expressed. ORF-4 encodes a probable helix-turn-helix-containing DNA-binding protein, given the presence of the helix-turn-helix motif, characteristic of this class of proteins. Its N-terminal region has high identity with its counterparts from proteins belonging to the RRF2 family. Northern blot analysis shows that ORF-4 is transcribed as a single mRNA in contrast to its orthologue from Desulfovibrio vulgaris. ORF-5 encodes a putative fusion protein as its N- and C-termini show significant homologies with molybdenum formylmethanofuran dehydrogenase and molybdopterin biosynthesis proteins, respectively. ORF-7 encodes a prokaryotic lipoprotein having homologies with multidrug efflux and DNA damage-inducible proteins, and it is constitutively and abundantly expressed.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"18 1","pages":"229 - 238"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83824918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084455
Ezhou L. Long, A. Capuco, Xin Zhao
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.
{"title":"Cloning of Bovine Eukaryotic Translation Initiation Factor 4E (eIF-4E) and Its Expression in the Bovine Mammary Gland at Different Physiological Stages","authors":"Ezhou L. Long, A. Capuco, Xin Zhao","doi":"10.3109/10425170109084455","DOIUrl":"https://doi.org/10.3109/10425170109084455","url":null,"abstract":"Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"57 1","pages":"319 - 329"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85619664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041333
D. Panetta, L. Yin, R. Barale, G. Romeo, R. Ravazzolo, Isabella Ceccherinp, A. Pulit
The RET proto-oncogene is involved in the development of both kidney and neural crests derived tissues. RET deleterious mutations cause hereditary neuroendocrine tumours and congenital intestinal agangliono-sis. Ongoing efforts aimed at elucidating the function of this gene include expression studies in different species and in transgenic mice. As first step in the study of Ret expression in mouse, we obtained the mouse Ret genomic structure. Intron-exon boundaries were determined and sequenced, all introns but the first one were amplified and cloned, and exons positioned in a restriction map. Mouse and human genes comparison indicates a highly conserved genomic organisation, except for exon 21 which is not conserved in mouse. A region extending 386 bp 5′ to the first exon was sequenced and compared with its human counterpart. Some features, reported for the human promoter, like the absence of TATA or CAAT boxes and a high GC content, are conserved.
RET原癌基因参与肾脏和神经嵴衍生组织的发育。RET有害突变可引起遗传性神经内分泌肿瘤和先天性肠神经节病。正在进行的旨在阐明该基因功能的工作包括在不同物种和转基因小鼠中的表达研究。作为小鼠Ret表达研究的第一步，我们获得了小鼠Ret基因组结构。确定内含子和外显子的边界并测序，扩增和克隆除第一个内含子外的所有内含子，并将外显子定位在限制子图中。小鼠与人类基因比较表明，除外显子21在小鼠中不保守外，其他基因组组织高度保守。测序了一个延伸至第一个外显子386 bp 5 '的区域，并与人类的对应区域进行了比较。据报道，人类启动子的一些特征，如没有TATA或CAAT盒和高GC含量，是保守的。
{"title":"Genomic Organisation of the Mouse Ret Proto-Oncogene","authors":"D. Panetta, L. Yin, R. Barale, G. Romeo, R. Ravazzolo, Isabella Ceccherinp, A. Pulit","doi":"10.3109/10425170109041333","DOIUrl":"https://doi.org/10.3109/10425170109041333","url":null,"abstract":"The RET proto-oncogene is involved in the development of both kidney and neural crests derived tissues. RET deleterious mutations cause hereditary neuroendocrine tumours and congenital intestinal agangliono-sis. Ongoing efforts aimed at elucidating the function of this gene include expression studies in different species and in transgenic mice. As first step in the study of Ret expression in mouse, we obtained the mouse Ret genomic structure. Intron-exon boundaries were determined and sequenced, all introns but the first one were amplified and cloned, and exons positioned in a restriction map. Mouse and human genes comparison indicates a highly conserved genomic organisation, except for exon 21 which is not conserved in mouse. A region extending 386 bp 5′ to the first exon was sequenced and compared with its human counterpart. Some features, reported for the human promoter, like the absence of TATA or CAAT boxes and a high GC content, are conserved.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"69 1","pages":"501 - 506"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89105071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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