Pub Date : 2001-01-01DOI: 10.3109/10425170109025001
S. Dühr, A. Torres-Montaner, A. Astola, F. García-Cózar, C. Pendón, J. Bolívar, M. Valdivia
Upstream binding factor, UBF, is a nucleolar autoantigen involved in the transcription of ribosomal DNA genes. Previously, human genomic clones served to demonstrate that an alternative pre-mRNA splicing of a single gene is used to form UBF1 and UBF2. Here, to complete characterizing the 5′end genomic organization of this nucleolar transcription factor, lambda clones containing the human UBF gene were isolated from a human placenta genomic library using a hamster UBF cDNA as a probe. An additional PCR product was isolated from HeLa genomic DNA to cover the first translated 60 nt of the gene containing the ATG initiation codon. We have also determined the transcription start site of the gene by primer extension analysis at nt 188 upstream from the start ATG codon. It served first, to identify an untranslated initial exon on the UBF gene covering the first 121 nt of human UBF cDNA, and also to establish the sequence of the proximal promoter. The human UBF promoter lacks a TATA and CAAT boxes but contains multiple binding sites for SP1, API, AP2, TFIID, NF-1 and a single site for NFAT-1. Consequently we have defined the first five exons of the human UBF gene covering 7.5 kb. The complete gene now consists of 20 exons with intervening sequences and spans approximately 15kb of DNA.
{"title":"Short Communication: Molecular Analysis of the 5′ Region of Human Ribosomal Transcription Factor UBF","authors":"S. Dühr, A. Torres-Montaner, A. Astola, F. García-Cózar, C. Pendón, J. Bolívar, M. Valdivia","doi":"10.3109/10425170109025001","DOIUrl":"https://doi.org/10.3109/10425170109025001","url":null,"abstract":"Upstream binding factor, UBF, is a nucleolar autoantigen involved in the transcription of ribosomal DNA genes. Previously, human genomic clones served to demonstrate that an alternative pre-mRNA splicing of a single gene is used to form UBF1 and UBF2. Here, to complete characterizing the 5′end genomic organization of this nucleolar transcription factor, lambda clones containing the human UBF gene were isolated from a human placenta genomic library using a hamster UBF cDNA as a probe. An additional PCR product was isolated from HeLa genomic DNA to cover the first translated 60 nt of the gene containing the ATG initiation codon. We have also determined the transcription start site of the gene by primer extension analysis at nt 188 upstream from the start ATG codon. It served first, to identify an untranslated initial exon on the UBF gene covering the first 121 nt of human UBF cDNA, and also to establish the sequence of the proximal promoter. The human UBF promoter lacks a TATA and CAAT boxes but contains multiple binding sites for SP1, API, AP2, TFIID, NF-1 and a single site for NFAT-1. Consequently we have defined the first five exons of the human UBF gene covering 7.5 kb. The complete gene now consists of 20 exons with intervening sequences and spans approximately 15kb of DNA.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"23 1","pages":"267 - 272"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81643622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041332
A. A. Pedersen, N. Videbaek, K. Skak, H. V. Petersen, B. Michelsen
GAD65 and GAD67 are two isoforms of the enzyme glutamic acid decarboxylase which catalyze the production of GABA from glutamate, primarily in the brain. However, GAD and GABA also prevail in the retina, testes and islets of Langerhans. The main function of GABA is in neurotransmission, and it is involved in paracrine signalling in islets, but has also been suggested to play a role as a trophic factor in syn-aptogenesis and to be an important metabolite feeding into the tricarboxylic acid cycle via the GABA-shunt. Both GAD isoforms are subject to regulation, e.g. by synaptic activity. GAD65 is regulated at the level of enzyme activity by association and dissociation from its cofactor, PLP, whereas GAD67 is controlled at the level of its mRNA. To study this process in further detail, we have isolated and characterized the 5′-flank-ing region of the rat GAD67 gene. We report the transcriptional initiation sites and promoter sequences important for expression in islet P-cells and C6 glioma cells, and demonstrate that the GAD67 promoter harbors elements that are responsive to glucose in primary islet cells.
{"title":"Characterization of the Rat Gad67 Gene Promoter Reveals Elements Important for Basal Transcription and Glucose Responsiveness","authors":"A. A. Pedersen, N. Videbaek, K. Skak, H. V. Petersen, B. Michelsen","doi":"10.3109/10425170109041332","DOIUrl":"https://doi.org/10.3109/10425170109041332","url":null,"abstract":"GAD65 and GAD67 are two isoforms of the enzyme glutamic acid decarboxylase which catalyze the production of GABA from glutamate, primarily in the brain. However, GAD and GABA also prevail in the retina, testes and islets of Langerhans. The main function of GABA is in neurotransmission, and it is involved in paracrine signalling in islets, but has also been suggested to play a role as a trophic factor in syn-aptogenesis and to be an important metabolite feeding into the tricarboxylic acid cycle via the GABA-shunt. Both GAD isoforms are subject to regulation, e.g. by synaptic activity. GAD65 is regulated at the level of enzyme activity by association and dissociation from its cofactor, PLP, whereas GAD67 is controlled at the level of its mRNA. To study this process in further detail, we have isolated and characterized the 5′-flank-ing region of the rat GAD67 gene. We report the transcriptional initiation sites and promoter sequences important for expression in islet P-cells and C6 glioma cells, and demonstrate that the GAD67 promoter harbors elements that are responsive to glucose in primary islet cells.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"76 1","pages":"485 - 499"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87131686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084455
Ezhou L. Long, A. Capuco, Xin Zhao
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.
{"title":"Cloning of Bovine Eukaryotic Translation Initiation Factor 4E (eIF-4E) and Its Expression in the Bovine Mammary Gland at Different Physiological Stages","authors":"Ezhou L. Long, A. Capuco, Xin Zhao","doi":"10.3109/10425170109084455","DOIUrl":"https://doi.org/10.3109/10425170109084455","url":null,"abstract":"Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"57 1","pages":"319 - 329"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85619664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109080770
H. Mayer, Martina Pongratz, R. Prohaska
We identified and characterized the cDNA coding for human LANCL2, a new member of the eukaryotic LanC-like protein family which is related to the bacterial lanthionine synthetase components C (LanC). The composite nucleotide sequence revealed a coding region of 1353 bp, a 5′-UTR of 186 bp and a 3'-UTR of 2421 bp. The deduced sequence of 450 amino acids showed 57.9 % identity (74.7 % similarity) when compared with the human LANCL1 homologue. In contrast to LANCL1, a unique ATP/GTP-binding site motif A was found in LANCL2. Northern blot analysis revealed the presence of two major transcripts in the brain, 4.7 kb and 4.1 kb in size, and a major 1.8 kb transcript in testis. Accordingly, expression array analysis showed prominent signals in these tissues. Because of the structural similarity to LanC, we postulate that LANCL2 may play a role as a component of a pep-tide-modifying complex.
{"title":"Molecular Cloning, Characterization, and Tissue-Specific Expression of Human LANCL2, a Novel Member of the LanC-Like Protein Family","authors":"H. Mayer, Martina Pongratz, R. Prohaska","doi":"10.3109/10425170109080770","DOIUrl":"https://doi.org/10.3109/10425170109080770","url":null,"abstract":"We identified and characterized the cDNA coding for human LANCL2, a new member of the eukaryotic LanC-like protein family which is related to the bacterial lanthionine synthetase components C (LanC). The composite nucleotide sequence revealed a coding region of 1353 bp, a 5′-UTR of 186 bp and a 3'-UTR of 2421 bp. The deduced sequence of 450 amino acids showed 57.9 % identity (74.7 % similarity) when compared with the human LANCL1 homologue. In contrast to LANCL1, a unique ATP/GTP-binding site motif A was found in LANCL2. Northern blot analysis revealed the presence of two major transcripts in the brain, 4.7 kb and 4.1 kb in size, and a major 1.8 kb transcript in testis. Accordingly, expression array analysis showed prominent signals in these tissues. Because of the structural similarity to LanC, we postulate that LANCL2 may play a role as a component of a pep-tide-modifying complex.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"110 1","pages":"161 - 166"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87684571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109042053
Jae-Seong Lee, M. Gye
Intron 2 of the β-actin genes from several fish species was sequenced and aligned with a Clustal W program for maximum similarity. From the maximum similarity and phylip data, it was noted that the intron 2 sequences would be useful for establishing a molecular phylogenetic tree and in elucidating the gene flow through evolution, especially at the family level.
{"title":"Use of β-Actin Gene Intron 2 as a Phylogenetic Marker in Fish Taxonomy","authors":"Jae-Seong Lee, M. Gye","doi":"10.3109/10425170109042053","DOIUrl":"https://doi.org/10.3109/10425170109042053","url":null,"abstract":"Intron 2 of the β-actin genes from several fish species was sequenced and aligned with a Clustal W program for maximum similarity. From the maximum similarity and phylip data, it was noted that the intron 2 sequences would be useful for establishing a molecular phylogenetic tree and in elucidating the gene flow through evolution, especially at the family level.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"10 1","pages":"71 - 76"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81982362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109047561
Laura Carim-Todd, L. Sumoy, N. Andreu, X. Estivill, M. Escarceller
We have identified C15orf4, a novel human gene showing homology to the yeast mitochondrial ribosomal protein YmL30. C15orf4 encodes a transcript of 1,006 nt with an ORF of 279 amino acids and a predicted protein size of 31.7 kDa. Expression of C15orf4 is enriched in testis. C15orf4 was positioned to chromosome 15q24 by radiation hybrid mapping. We have identified the C15orf4 mouse orthologue as well as homologues in other species.
{"title":"Cloning, Mapping and Expression Analysis of C15 or f4, a Novel Human Gene with Homology to the Yeast Mitochondrial Ribosomal Protein Yml30 Gene","authors":"Laura Carim-Todd, L. Sumoy, N. Andreu, X. Estivill, M. Escarceller","doi":"10.3109/10425170109047561","DOIUrl":"https://doi.org/10.3109/10425170109047561","url":null,"abstract":"We have identified C15orf4, a novel human gene showing homology to the yeast mitochondrial ribosomal protein YmL30. C15orf4 encodes a transcript of 1,006 nt with an ORF of 279 amino acids and a predicted protein size of 31.7 kDa. Expression of C15orf4 is enriched in testis. C15orf4 was positioned to chromosome 15q24 by radiation hybrid mapping. We have identified the C15orf4 mouse orthologue as well as homologues in other species.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"24 1","pages":"91 - 96"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78415378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109047568
V. H. Liao, J. Freedman
A homologue of pyruvate carboxylase was isolated as an expressed sequence tag during the identification of cadmium-responsive genes in Caenorhabditis elegans. The C. elegans protein, designated PYC-1, is predicted to contain 1,175 amino acids with a molecular mass of 129,284. Amino acid sequence analysis indicates that PYC-1 will be translocated into mitochondria. PYC-1 has high levels of amino acid sequence identity with other pyruvate carboxylases. The highest levels of identity are in the putative transcarboxylation, biotin carboxylation and biotin carboxyl carrier domains.
{"title":"Characterization of a Cadmium-Inducible Isoform of Pyruvate Carboxylase from Caenorhabditis elegans","authors":"V. H. Liao, J. Freedman","doi":"10.3109/10425170109047568","DOIUrl":"https://doi.org/10.3109/10425170109047568","url":null,"abstract":"A homologue of pyruvate carboxylase was isolated as an expressed sequence tag during the identification of cadmium-responsive genes in Caenorhabditis elegans. The C. elegans protein, designated PYC-1, is predicted to contain 1,175 amino acids with a molecular mass of 129,284. Amino acid sequence analysis indicates that PYC-1 will be translocated into mitochondria. PYC-1 has high levels of amino acid sequence identity with other pyruvate carboxylases. The highest levels of identity are in the putative transcarboxylation, biotin carboxylation and biotin carboxyl carrier domains.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"82 1","pages":"137 - 145"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72712961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109025002
K. Adolph
Because of the close proximity of D. melanogaster gene CG11327 and the gene for a thrombospondin homologue (DTSP), the relationship between transcription of the two genes has been investigated. As part of this study, the cDNA sequence of gene CG11327 was determined, and structural features of the encoded protein were characterized. The CG11327 open reading frame specifies a polypeptide of 475 amino acid residues, molecular weight 53,666. The highly acidic protein has an aspartic acid+glutamic acid content of 23.2%. Weak similarities to other proteins suggest that it may function as a transcription factor or structural protein of the cell cycle. The existence of overlapping transcripts was found to be a significant feature of transcription of gene CG11327 and the DTSP gene. The 3′ end of the DTSP gene overlaps the 5′ end of gene CG11327 by at least 177 bp, and the region of overlap includes exon 13 and the 3′ UTR of the DTSP gene.
{"title":"Short Communication: Relationship of Transcription of Drosophila melanogaster Gene CG11327 and the Gene for a Thrombospondin Homologue (DTSP)","authors":"K. Adolph","doi":"10.3109/10425170109025002","DOIUrl":"https://doi.org/10.3109/10425170109025002","url":null,"abstract":"Because of the close proximity of D. melanogaster gene CG11327 and the gene for a thrombospondin homologue (DTSP), the relationship between transcription of the two genes has been investigated. As part of this study, the cDNA sequence of gene CG11327 was determined, and structural features of the encoded protein were characterized. The CG11327 open reading frame specifies a polypeptide of 475 amino acid residues, molecular weight 53,666. The highly acidic protein has an aspartic acid+glutamic acid content of 23.2%. Weak similarities to other proteins suggest that it may function as a transcription factor or structural protein of the cell cycle. The existence of overlapping transcripts was found to be a significant feature of transcription of gene CG11327 and the DTSP gene. The 3′ end of the DTSP gene overlaps the 5′ end of gene CG11327 by at least 177 bp, and the region of overlap includes exon 13 and the 3′ UTR of the DTSP gene.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"13 1","pages":"273 - 279"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74782920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041340
N. Makino, T. Yamato, H. Inoue, T. Furukawa, T. Abe, T. Yokoyama, T. Yatsuoka, S. Fukushige, Seiichi Orikasa, Tsuneo Takahashi, A. Horii
Deletions of the long arm of chromosome 6 (6q) are one of the most common chromosomal abnormalities in multiple human malignancies. Previously, we have identified three commonly deleted regions on 6q (6q21, 6q23-q24, and 6q26) in pancreatic cancer by loss of heterozygosity studies, suggesting the presence of one or more tumor suppressor genes on this chromosome arm. Using a combination of database search and cDNA library screening, we successfully isolated a transcript from 6q24. This mRNA encodes a protein consisting of 543 amino acids with homology to the Drosophila headcase (hdc) gene and, thus, is designated as hHDC. Northern analysis identified a ubiquitously expressed 5.6-kb transcript. Seventeen (81%) of 21 pancreatic cancer cell lines and four (80%) of five renal cell carcinoma cell lines showed low level expression, suggesting that the hHDC gene may play an important role in some human cancers.
{"title":"Isolation and Characterization of the Human Gene Homologous to the Drosophila Headcase (hdc) Gene in Chromosome Bands 6q23-q24, a Region of Common Deletion in Human Pancreatic Cancer","authors":"N. Makino, T. Yamato, H. Inoue, T. Furukawa, T. Abe, T. Yokoyama, T. Yatsuoka, S. Fukushige, Seiichi Orikasa, Tsuneo Takahashi, A. Horii","doi":"10.3109/10425170109041340","DOIUrl":"https://doi.org/10.3109/10425170109041340","url":null,"abstract":"Deletions of the long arm of chromosome 6 (6q) are one of the most common chromosomal abnormalities in multiple human malignancies. Previously, we have identified three commonly deleted regions on 6q (6q21, 6q23-q24, and 6q26) in pancreatic cancer by loss of heterozygosity studies, suggesting the presence of one or more tumor suppressor genes on this chromosome arm. Using a combination of database search and cDNA library screening, we successfully isolated a transcript from 6q24. This mRNA encodes a protein consisting of 543 amino acids with homology to the Drosophila headcase (hdc) gene and, thus, is designated as hHDC. Northern analysis identified a ubiquitously expressed 5.6-kb transcript. Seventeen (81%) of 21 pancreatic cancer cell lines and four (80%) of five renal cell carcinoma cell lines showed low level expression, suggesting that the hHDC gene may play an important role in some human cancers.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"5 1","pages":"547 - 553"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75486329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084475
L. Stamm, H. Bergen
A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.
对齿状密螺旋体4.2 kb DNA区包含4个完整基因(orf1、fliQ、fliR和flhB)和一个截断基因(flhA1)进行测序和分析。flq、FliR、FlhB和FlhA'的氨基酸序列与鞭毛输出器相关的细菌蛋白具有显著的同源性，而Orfl的氨基酸序列与大肠杆菌乙醇脱氢酶具有显著的同源性。在fliQ上游发现了一个推测的α70样启动子。RT-PCR分析表明，fliQ、fliR、flhB和flhA1独立于orfl共转录，提示运动相关基因是一个操纵子的组成部分。denticola T. fliQ-flhA1基因与对应的T. pallidum和Borrelia burgdorferi基因的位置不同，后者分别存在于大鞭毛操纵子或flgB鞭毛操纵子中。
{"title":"Molecular Characterization of the Treponema denticola fliQ Region","authors":"L. Stamm, H. Bergen","doi":"10.3109/10425170109084475","DOIUrl":"https://doi.org/10.3109/10425170109084475","url":null,"abstract":"A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"2 1","pages":"463 - 467"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88972969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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