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Cytopathological Impacts of Certain Plant Growth Regulators on The Circulating Hemocytes of Galleria mellonella L. (Lepidoptera: Pyralidae) 某些植物生长调节剂对黄颡鱼蛾循环血细胞的细胞病理学影响
Pub Date : 2022-09-20 DOI: 10.21608/eajbsa.2022.259860
Hamadah, Kh., G. K., El-Hela, A., A. A.
The greater wax moth Galleria mellonella is a worldwide insect pest damaging wax combs and feeding on bee‐hive products. The objective of the current study was to investigate the cytopathological impacts of four plant growth regulators (PGRs), viz ., indole-3-acetic acid, indole-3-butyric acid, 2,4-Dichlorophenoxy acetic acid and 6-benzyladenine, on the circulating hemocytes of last (7 th ) instar larvae of G. mellonella . For this purpose, the 3 rd instar larvae were force-fed on diet supplemented with LC 50 values of these PGRs (0.24, 0.022, 0.16 & 0.085 ppm, respectively). These larvae were continuously fed on the treated diet throughout the larval stage. The important results could be summarized as follows. Five main types of the freely circulating hemocytes in the haemolymph of larvae had been identified as Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). Different qualitative disorders of the profile of each circulating hemocyte type were demonstrated in thelast instar larvae, such as destruction of cell nuclei and/or membrane, some extruded cytoplasmic contents, and production of some vacuoles in the cytoplasm. However, some of the tested PGRs failed to exhibit cytopathological impacts on certain hemocytes, since IAA and 6-BA failed to affect PLs, IBA and 6-BA failed to affect GRs, IBA and 6-BA failed to affect SPs and IBA and 6-BA failed to affect OEs. Therefore, IAA and 2,4-D may be recommended to use in the IPM program against G. mellonella
大蜡蛾(Galleria mellonella)是一种世界范围内的害虫,它破坏蜂窝,以蜂巢产品为食。本研究旨在探讨吲哚-3-乙酸、吲哚-3-丁酸、2,4-二氯苯氧乙酸和6-苄基腺嘌呤四种植物生长调节剂(PGRs)对大蜡螟末龄(7龄)幼虫循环血细胞的细胞病理学影响。为此,将3龄幼虫强制饲喂添加这些pgr的lc50值(分别为0.24、0.022、0.16和0.085 ppm)的日粮。这些幼虫在整个幼虫期连续饲喂处理过的饲料。重要结果可总结如下:幼虫血淋巴中自由循环的血细胞主要有原血细胞(PRs)、浆细胞(PLs)、粒细胞(GRs)、球形细胞(SPs)和嗜酸细胞(OEs)五种类型。不同类型的循环血细胞在末龄幼虫中表现出不同的定性紊乱,如细胞核和/或细胞膜的破坏,细胞质内容物的挤压,细胞质中产生一些液泡。然而,一些测试的pgr对某些血细胞没有表现出细胞病理学影响,因为IAA和6-BA不能影响PLs, IBA和6-BA不能影响GRs, IBA和6-BA不能影响SPs, IBA和6-BA不能影响OEs。因此,IAA和2,4- d可能被推荐用于抗葡萄球菌的IPM计划
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引用次数: 0
Pauesia silanaTremblay, 1969 A Parasitoid of Cinara maghrebica-Aphid in Aleppo Pine Forests in Algeria Case Khenchela Province 阿尔及利亚凯肯切拉省阿勒颇松林马格里布蚜的寄生蜂
Pub Date : 2022-09-19 DOI: 10.21608/eajbsa.2022.259859
A. Agronomy, Laamari, Agronomy, Tahar-chaouche, Agronomy
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引用次数: 0
Application of Entomopathogenic Nematodes to Control Larvae of Temnorhynchus baal (Reiche and Saulcy) Under Laboratory Conditions and In Strawberry Fields 昆虫病原线虫在草莓田和实验室条件下防治巴力夜蛾幼虫的应用
Pub Date : 2022-08-24 DOI: 10.21608/eajbsa.2022.256736
D. Adly, G. Nouh, A. Eid
The white grub, Temnorhynchus baal, is a major pest of the strawberry crop in many Asian and African countries. The white grabs cause significant economic losses in the yield. The present study aims to evaluate the effectiveness of entomopathogenic nematodes (EPNs) on the third larval instar of T. baal under laboratory conditions. The study extended to compare the efficacy of a combination of EPNs ( Steinernema glaseri + Heterorhabditis bacteriophora ) against a natural infestation of T. baal with an organophosphorus insecticide (Diazinon) in an Egyptian strawberry field. In the laboratory, five larvae of T. baal were placed in soil and infected with EPNs. At the highest concentration of 4000 IJs/larva, the larvae were more susceptible to infection with S. glaseri than H. bacteriophora , with mortality percentages of 96 and 88%, respectively. The LC50 of S. glaseri and H. bacteriophora was 937.44 and 1026.58 IJs/larvae, respectively. The mortality percentage was 96% and 100% when treated for five larvae/cup and one larva/cup, respectively, after being infected with a combination of S. glaseri + H. bacteriophora . The mortality percentage was higher in the mixture than for each species studied individually. In the field, throughout the seasons, the percentage of wilted plants was 17.64, 66.09, and 83.95% in 2020 and 12.56, 67.87, and 75.62% in 2021, for the plots treated with a combination of EPNs, insecticides, and control, respectively. Present findings indicate that entomopathogenic nematodes are good alternatives to control the white grub, T. baal , in strawberry fields.
在许多亚洲和非洲国家,白蛴螬是草莓作物的主要害虫。白抢造成了重大的产量经济损失。本研究旨在评价昆虫病原线虫(EPNs)在实验室条件下对巴力小蠊第三龄幼虫的杀灭效果。该研究扩展到比较EPNs (glasernema + Heterorhabditis bacteriophora)组合在埃及草莓田对抗T. baal自然侵染与有机磷杀虫剂(Diazinon)的效果。在实验室中,将5只巴力绦虫幼虫置于土壤中感染EPNs。在最高浓度为4000 IJs/幼虫时,幼虫对glaseri s的易感程度高于嗜杆菌h,死亡率分别为96%和88%。glaseri和bacteriophora的LC50分别为937.44和1026.58 IJs/幼虫。glaseri s +嗜杆菌h联合感染后,5只/杯和1只/杯处理的死亡率分别为96%和100%。混合后的死亡率高于单独研究的每一种。在田间,各季节EPNs、杀虫剂和防治组合处理的枯萎率在2020年分别为17.64、66.09和83.95%,在2021年分别为12.56、67.87和75.62%。研究结果表明,昆虫病原线虫是防治草莓田白蛴螬的较好选择。
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引用次数: 0
Comparative Amino Acid Content of Pseudocanthotermes grandiceps, Macrotermis jeanelli and Allodontermes tenax (Isoptera: Termitidea) 大白蚁、大白蚁和小白蚁氨基酸含量比较(等翅目:白蚁总目)
Pub Date : 2022-08-21 DOI: 10.21608/eajbsa.2022.256374
Makila Jacob, Omukunda Elizabeth, Ndong’a Millicent
The main objective of this study was to compare the amino acid profiles of P.grandiceps, M. jeanelli and A. tenax. The study was carried out in Trans-Nzoia at an altitude of 1,900 meters, with a latitude of 1°1'8.72"N, and longitude of 35°0'8.3"E. Termite alates was collected when swarming and placed in 500grams collection jars and taken to the laboratory for amino acid analysis. Some of the alates and a number of small or large soldiers and workers were preserved in 80% ethanol for identification at the National Museums of Kenya. The collected termite alates were analyzed for composition of amino acids using standard methods detailed in AOAC (2000). From the results, there was no significant difference in amino acid content, amino acid classes, amino acid groups and total essential amino acids and total non-essential amino acids of the three termite species. Glutamic acid was the most abundant of the three species. It ranged between 8.43g/100g -15.5g/100g. The lowest concentrated amino acid was cysteine ranging between 0.60g/100g- 3.92g/100g. Leucine was found to be the most highly concentrated essential amino acid (eaa); 7.72g/100g crude protein in P. grandiceps , 7.48g/100g in M. jeanelli and 5.51g/100g in A. tenax . Methionine was the lowest eaa in P. grandiceps at 0.88g/100g crude protein while Histidine was the lowest eaa in both M. jeanelli and A. tenax at 2.15g/100g and 2.09g/100g respectively.
本研究的主要目的是比较大头菜、吉纳利菜和藤菜的氨基酸谱。这项研究是在海拔1 900米、北纬1°1′8.72”、东经35°0′8.3”的跨- nzoia进行的。蚁群时采集白蚁唾液,装入500克采集罐中,送往实验室进行氨基酸分析。一些尸体和一些大小不一的士兵和工人被保存在80%的乙醇中,以便在肯尼亚国家博物馆进行鉴定。采用AOAC(2000)的标准方法对采集的白蚁食材进行氨基酸组成分析。结果表明,三种白蚁的氨基酸含量、氨基酸类别、氨基酸基团以及总必需氨基酸和总非必需氨基酸均无显著差异。其中谷氨酸含量最高。它的范围在8.43g/100g - 15.g /100g之间。氨基酸浓度最低的是半胱氨酸,介于0.60g/100g ~ 3.92g/100g之间。发现亮氨酸是最高浓度的必需氨基酸(eaa);粗蛋白含量为7.72g/100g,粗蛋白含量为7.48g/100g,粗蛋白含量为5.51g/100g。粗蛋白质中蛋氨酸的eaa最低,为0.88g/100g;组氨酸的eaa最低,分别为2.15g/100g和2.09g/100g。
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引用次数: 0
Study of the Colonization Behaviour of Stored Grains Pest, Sitophilus oryzae L. (Coleoptera: Curculionidae) Through Determining the Colour Preference. 储粮害虫米象虫定殖行为的颜色偏好研究(鞘翅目:粟象科)。
Pub Date : 2022-08-21 DOI: 10.21608/eajbsa.2022.255521
A. M.
Article History is The current research aimed to study the colour preferences of S . oryzae through the free choice test method, the preference of adult insects to different colour cues was evaluated with a multi-choice test 24 hours after insect release. The research was conducted with three replicates using six colour cues; red, white, black, yellow, green, and blue under laboratory conditions. The observed parameter was the number of adults attracted to the different colour cues. The results showed that the highest preference of S. oryzae was recorded in the green and red colour cues, while the white colour was the least preferred colour. These results could be helpful in the integrated pest management programs to control the stored product insect pest S . oryzae .
本文的研究目的是研究S的颜色偏好。采用自由选择试验法,在释放后24 h采用多项选择试验评价成虫对不同颜色线索的偏好。研究进行了三次重复,使用六种颜色线索;红色,白色,黑色,黄色,绿色和蓝色在实验室条件下。观察到的参数是被不同颜色吸引的成虫的数量。结果表明,稻瘟病菌对绿色和红色线索的偏好最高,对白色线索的偏好最低。研究结果可为储粮害虫的综合防治提供依据。oryzae。
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引用次数: 0
Biochemical Composition, Toxicity and Bioactivities of the Essential Oil extracted from Coffea arabica L. husks against the Cotton Leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctudiae) 阿拉比卡咖啡豆皮精油对棉花叶虫的生化成分、毒性及生物活性研究
Pub Date : 2022-08-05 DOI: 10.21608/eajbsa.2022.254995
Eman E. Essa, Gawhara M. M. Abu El-Hassan, S. Farag
throughout Therefore, approaches for their a ) analyzed its were screened for insecticidal activity against the larval instars of the littoralis GC/MS analysis revealed that the arabic coffee included 13 compounds of which Oleic acid comprises the largest proportion 65 % which is known for its insecticidal efficacy. Other compounds that have insecticidal activities were also separated; Cyclononasiloxane, octadecamethyl-, n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z, Z)- and N-Isobutyl-11-(3,4-methylenedioxyphenyl)-2E,4E,10E-undecatrienoic amide. The oil extract was tested against the 4 th larval instars. The results indicated the efficacy of the extract as larvicide with the values of LC 50 and LC 90 equal to 1.8083 and 8.3227 respectively. The application of the oil extract against the larvae resulted in the prolongation of both larval and pupal duration and the decrease of the growth rate, pupation, adult longevity and fecundity. Also, the corrected weight of consumed leaf, consumption index (IC), relative growth rate (RGR), the efficacy of ingested and digested food conversion to body tissue (ECI) and (ECD), respectively and the approximate digestibility (AD) were decreased after the application of the oil extract on the larvae. As a result of the current study, the oil extract from the husk of Coffea arabica revealed its efficacy as both larvicide and deterrent against the 4 th instar larvae of cotton leafworm S. littoralis .
因此,对其a)分析方法进行了对海蛾幼虫的杀虫活性筛选,GC/MS分析显示,阿拉伯咖啡含有13种化合物,其中油酸所占比例最大,为65%,以其杀虫功效闻名。其他具有杀虫活性的化合物也被分离出来;环壬硅氧烷,十八甲基-,正十六烷酸,9,12-十八二烯酸(Z, Z)-和n-异丁基-11-(3,4-亚甲基二氧苯)- 2e,4E, 10e -十一烯酰胺。对油浸提液对4龄幼虫进行了抑虫试验。结果表明,黄芪提取物的lc50和lc90分别为1.8083和8.3227,具有较好的杀幼虫效果。油浸液对幼虫的处理使幼虫期和蛹期均延长,生长速度、化蛹率、成虫寿命和繁殖力均降低。此外,油浸提物还降低了采食叶的校正重、采食指数(IC)、相对生长率(RGR)、采食和消化食物转化为体组织的效率(ECI)和ECD以及近似消化率(AD)。本研究结果表明,阿拉比卡咖啡皮油提取物对棉花叶虫4龄幼虫具有杀幼虫和抑制作用。
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引用次数: 0
The Effect of Onion Powder (Allium Sp.) As An Antibiotic on The Larval Haemolymph of Silkworm and Its Effect on The Economic Parameters of Cocoons as A Food Supplementary 洋葱粉作为抗生素对家蚕幼虫血淋巴的影响及其对蚕茧辅食经济指标的影响
Pub Date : 2022-08-02 DOI: 10.21608/eajbsa.2022.251819
Shimaa, Shakl
The study aims to use natural antimicrobial such as onion powder ( Allium sp .) which has an effective role as a strong antibiotic to raise the immunity of sensitive silkworm larvae to infection with many diseases. Three doses of Onion powder were used 5,10 and 15gm, and the following was studied: The economic parameters of the cocoons and the weight of silkworm larvae of the fifth age and the effect of using the onion powder on these traits were studied. The results showed that the maximum weight of the larvae was in the dose of 15 gm while the dose of 10 gm was the highly increment in the weight of cocoon, cocoon shell weight and the shell ratio. It concluded that the use of 5 and 10gm from Onion Powder as supplementary feeding on the mulberry leaves enhanced the immunology of larvae without affecting the quality of the economic parameters of the cocoons.
本研究旨在利用洋葱粉(Allium sp .)等天然抗菌剂作为强抗生素,有效提高敏感家蚕幼虫对多种疾病感染的免疫力。采用5、10、15gm三种剂量的洋葱粉,研究了5龄蚕茧的经济参数和幼虫的体重,并研究了洋葱粉对这些性状的影响。结果表明,15 gm剂量时幼虫体重最大,10 gm剂量对茧重、茧壳重和壳比均有较大的增加。综上所述,在不影响蚕茧经济参数质量的前提下,用5和10gm洋葱粉对桑叶进行补饲可增强幼虫的免疫功能。
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引用次数: 0
Histological Changes in the Adult Seed Bug, Graptostethus servus (Hemiptera: Lygaeidae) Treated with the Entomopathogenic Fungus, Beauveria bassiana (Ascomycota: Hypocreales). 球孢白僵菌(子囊菌目:下毛囊目)对成虫(半翅目:夜蛾科)的组织学改变。
Pub Date : 2022-08-01 DOI: 10.21608/eajbsa.2022.251638
M. Nada, A. Gad, A. Soliman
---------------------Keywords is very vulnerable to B. bassiana. LC 50 value was 2.9x10 6 spores/ml and the LT 50 value was 4.16 days. The infection affected various body parts, especially the cuticle and the midgut. The application of B. bassiana on the adultʼs cuticle resulted in an apparent disturbance in the structure of the cuticle and degeneration of its different parts. These changes were observed 48 hours after treatment and increased in severity over time. The fungal infection was not limited to the cuticle, but also affected the epithelial cells of the crop and midgut, as well as the mesenteric caeca. These results highlight the insecticidal potential of the fungus B. bassiana against the pest G. servus .
--------------------- 关键词很容易单独使用。lc50值为2.9x10 6孢子/ml, l50值为4.16天。感染影响了身体的各个部位,尤其是角质层和中肠。球孢白僵菌施于成虫角质层后,对成虫角质层的结构造成明显的干扰,导致成虫角质层各部位的退化。这些变化在治疗后48小时观察到,并且随着时间的推移严重程度增加。真菌感染不仅限于角质层,而且还影响作物和中肠的上皮细胞以及肠系膜盲肠。这些结果突出了球孢白僵菌对害虫G. servus的杀虫潜力。
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引用次数: 0
New Record of The Billbug Genus Sphenophorus Schoenherr, 1838 (Coleoptera: Curculionidae: Dryophthorinae) From Egypt 埃及细耳虫属新记录,1838(鞘翅目:细耳虫科:细耳虫科)
Pub Date : 2022-07-19 DOI: 10.21608/eajbsa.2022.249408
Waleed El-Maghraby, Hala Kadada, Manal Elshaier
the specimen is billbug species of the genus Sphenophorus but they cannot confirm the species. During the present work, the specimens were described, photographed, and identified using the available taxonomic keys and previous descriptions. The taxonomic work confirmed that the specimens belong to Sphenophorus venatus Chittenden (hunting billbug) and were reported for the first time from Egypt. This finding with data about damage, distribution, and host plants contributes to enriching lists of Egypt’s insect fauna and conservation of biodiversity.
该标本为耳虫属的一种,但他们无法确定其种类。在目前的工作中,利用现有的分类键和先前的描述对标本进行了描述、拍照和鉴定。经分类鉴定,该标本属猎耳虫(Sphenophorus venatus Chittenden),为埃及首次报道。这一发现与有关损害、分布和寄主植物的数据有关,有助于丰富埃及昆虫动物群和保护生物多样性。
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引用次数: 0
First Records of Wahlgreniella nervata (Gillette, 1908) (Hemiptera : Aphididae) on Bell Pepper In The Region of Staoueli (Algeria) 阿尔及利亚Staoueli地区甜椒上第一次记录(吉列,1908)(半翅目:蚜虫科)
Pub Date : 2022-07-17 DOI: 10.21608/eajbsa.2022.249311
D. Lydia, H. Berrai, A. Abdul-Hussain, L. Saharaoui, M. Biche
{"title":"First Records of Wahlgreniella nervata (Gillette, 1908) (Hemiptera : Aphididae) on Bell Pepper In The Region of Staoueli (Algeria)","authors":"D. Lydia, H. Berrai, A. Abdul-Hussain, L. Saharaoui, M. Biche","doi":"10.21608/eajbsa.2022.249311","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.249311","url":null,"abstract":"","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"99 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72430088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Egyptian Academic Journal of Biological Sciences. A, Entomology
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