Using the example of a number of representatives of bacteria and archaea, the structure of their cell surface biopolymers is considered, taking into account post-translational modifications of proteins and contemporary views on the features of protein folding.
{"title":"Prokaryotic cell surface biopolymers: bioinformatic analysis","authors":"S. Shchyogolev, G. Burygin, M. Pyatibratov","doi":"10.28983/plamic2020.221","DOIUrl":"https://doi.org/10.28983/plamic2020.221","url":null,"abstract":"Using the example of a number of representatives of bacteria and archaea, the structure of their cell surface biopolymers is considered, taking into account post-translational modifications of proteins and contemporary views on the features of protein folding.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121117868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. D. Tobysheva, L. Khamidullina, I. S. Puzyrev, A. Pestov
The binding ability towards protein biomolecular targets have been analyzed for metal complexes based on polycarbonyl ligands using molecular docking.
利用分子对接的方法分析了聚羰基金属配合物对蛋白质生物分子靶标的结合能力。
{"title":"Biological activity of complexes based on polycarbonyl ligands: \u0000assessment of the mode of action using molecular docking","authors":"P. D. Tobysheva, L. Khamidullina, I. S. Puzyrev, A. Pestov","doi":"10.28983/plamic2020.249","DOIUrl":"https://doi.org/10.28983/plamic2020.249","url":null,"abstract":"The binding ability towards protein biomolecular targets have been analyzed for metal complexes based on polycarbonyl ligands using molecular docking.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128227876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Timergalin, A. Feoktistova, T. Rameev, S. P. Chetverikov, Z. R. Sultangazin
The effect of the identified auxin-producing strain of bacteria on wheat plants when treated with the herbicides Chistalan and Nanometh in the field was studied. The ability of bacterial treatment to increase wheat yield under herbicidal stress due to the positive effect of bacteria on plant growth and development at early stages of development is shown.
{"title":"Wheat yields of herbicide treatment along with auxin-producing bacteria Pseudomonas sp. DА1.2","authors":"M. Timergalin, A. Feoktistova, T. Rameev, S. P. Chetverikov, Z. R. Sultangazin","doi":"10.28983/plamic2020.246","DOIUrl":"https://doi.org/10.28983/plamic2020.246","url":null,"abstract":"The effect of the identified auxin-producing strain of bacteria on wheat plants when treated with the herbicides Chistalan and Nanometh in the field was studied. The ability of bacterial treatment to increase wheat yield under herbicidal stress due to the positive effect of bacteria on plant growth and development at early stages of development is shown.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127284189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.
{"title":"Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene","authors":"R. Taipova, B. R. Kuluev","doi":"10.28983/plamic2020.244","DOIUrl":"https://doi.org/10.28983/plamic2020.244","url":null,"abstract":"The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130547879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Syrova, V. Shakhnazarova, A. Shaposhnikov, A. Belimov, Y. Gogolev
The intensity of root colonization by phytopathogenic fungus and rhizobacterium differs depending on the tomato genotype. Inoculation of wild-type tomatoes Ailsa Craig, but not of its ABA deficient mutant flacca, with Novosphingobium sp. P6W inhibits root colonization by Fusarium oxysporum MF-G284.
{"title":"The intensity of root colonization by phytopathogenic fungus and rhizobacterium depends \u0000on the genotype of tomatoes and abscisic acid","authors":"D. Syrova, V. Shakhnazarova, A. Shaposhnikov, A. Belimov, Y. Gogolev","doi":"10.28983/plamic2020.242","DOIUrl":"https://doi.org/10.28983/plamic2020.242","url":null,"abstract":"The intensity of root colonization by phytopathogenic fungus and rhizobacterium differs depending on the tomato genotype. Inoculation of wild-type tomatoes Ailsa Craig, but not of its ABA deficient mutant flacca, with Novosphingobium sp. P6W inhibits root colonization by Fusarium oxysporum MF-G284.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134379975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The presence of non-pathogenic bacterial microflora in Arabidopsis thaliana suspension culture has been shown to coexist latently with plant cells for a long time. According to the results of 16S rRNA sequencing, it was shown that the detected bacteria belong to the species Rothia amarae. Using microbiological, microscopic and immunochemical methods, the correspondence of the obtained strain to the species Rothia amarae was also confirmed.
{"title":"Detection and primary characterization of Rothia amarae in a suspension culture of Arabidopsis thaliana (Heynh.) cells","authors":"A. Sokolov, L. Dykman, A. Galitskaya, O. Sokolov","doi":"10.28983/plamic2020.232","DOIUrl":"https://doi.org/10.28983/plamic2020.232","url":null,"abstract":"The presence of non-pathogenic bacterial microflora in Arabidopsis thaliana suspension culture has been shown to coexist latently with plant cells for a long time. According to the results of 16S rRNA sequencing, it was shown that the detected bacteria belong to the species Rothia amarae. Using microbiological, microscopic and immunochemical methods, the correspondence of the obtained strain to the species Rothia amarae was also confirmed.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117080553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. S. Yevstigneyeva, Y. P. Fedonenko, A. Shelud’ko
The biofilms of the bacteria A. brasilense and A. lipoferum are resistant to salt (over 400 mM NaCl in the medium) and temperature (up to 48° C) stresses. The nature of resistance to these stressors is determined by the type of biofilm.
细菌A. brasilense和A. lipoferum的生物膜耐盐(培养基中超过400 mM NaCl)和温度(高达48°C)胁迫。对这些压力源的抵抗性质是由生物膜的类型决定的。
{"title":"Biofilms of Azospirillum brasilense and Azospirillum lipoferum and their resistance to abiotic stresses","authors":"S. S. Yevstigneyeva, Y. P. Fedonenko, A. Shelud’ko","doi":"10.28983/plamic2020.282","DOIUrl":"https://doi.org/10.28983/plamic2020.282","url":null,"abstract":"The biofilms of the bacteria A. brasilense and A. lipoferum are resistant to salt (over 400 mM NaCl in the medium) and temperature (up to 48° C) stresses. The nature of resistance to these stressors is determined by the type of biofilm.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114462292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Sidarenka, H. A. Bareika, L. Valentovich, D. S. Paturemski, V. N. Kuptsou, M. Titok, E. Kalamiyets
Taxon-specific primers were developed and PCR conditions were optimized for diagnostics of bacterial and fungal plant pathogens. Methods for phytopathogens DNA isolation from plant material, soil and water were selected.
{"title":"Molecular diagnostics of bacterial and fungal plant diseases","authors":"A. V. Sidarenka, H. A. Bareika, L. Valentovich, D. S. Paturemski, V. N. Kuptsou, M. Titok, E. Kalamiyets","doi":"10.28983/plamic2020.229","DOIUrl":"https://doi.org/10.28983/plamic2020.229","url":null,"abstract":"Taxon-specific primers were developed and PCR conditions were optimized for diagnostics of bacterial and fungal plant pathogens. Methods for phytopathogens DNA isolation from plant material, soil and water were selected.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122244975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Valentovich, A. Muratova, Y. Shavela, M. Sikolenko, A. Akhremchuk
The main stages of work with perspective strains for protecting plants and animals from diseases are presented: genome sequencing and analysis, genetic construction of improved strains and checking their survival under model conditions.
{"title":"Sequencing and analysis of genomes of bacteria used to protect plants and animals from diseases","authors":"L. Valentovich, A. Muratova, Y. Shavela, M. Sikolenko, A. Akhremchuk","doi":"10.28983/plamic2020.264","DOIUrl":"https://doi.org/10.28983/plamic2020.264","url":null,"abstract":"The main stages of work with perspective strains for protecting plants and animals from diseases are presented: genome sequencing and analysis, genetic construction of improved strains and checking their survival under model conditions.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124171629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The results on the detection of changes in model microbial complexes formed in the rhizosphere of transgenic tobacco plants with enhanced antioxidant (FeSOD 1) and osmoprotective (codA) protection are presented.
{"title":"Variability of actinomycete complexes in the rhizosphere of transgenic and intact tobacco lines","authors":"I. Shirokikh, Y. Nazarova","doi":"10.28983/plamic2020.223","DOIUrl":"https://doi.org/10.28983/plamic2020.223","url":null,"abstract":"The results on the detection of changes in model microbial complexes formed in the rhizosphere of transgenic tobacco plants with enhanced antioxidant (FeSOD 1) and osmoprotective (codA) protection are presented.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122750586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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