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Culture Medium and Varietal Selection for Establishment of Callus Culture of Artemisia annua 黄花蒿愈伤组织培养的培养基及品种选择
Pub Date : 2018-12-12 DOI: 10.37134/EJSMT.VOL5.2.6.2018
C. Keong, Farah Alia Nordin, A. Bhatt, C. Keng
Plant tissue culture technology offers the potential of producing medicinally important secondary metabolites such as anti-malarial artemisinin from Artemisia annua. In this study, callus induction of three different varieties of A.annua, namely T1, T2 and Hi varieties was carried out using leaf explants on Murashige & Skoog (MS) and Litvay (LV) media with three different supplementations. MS medium added with 0.5 mg/L BA, 0.5 mg/L NAA and 0.5 g/L of casein hydrolysate (CH) induced the highest yield of callus biomass compared to the effect of picloram- or 2,4-enriched MS medium. T2 variety was found to be the highest yielding variety in this medium. Picloram-enriched MS medium induced better callus in term of callus biomass and friability than 2,4-enriched medium. Hi variety induced in MS medium added with 0.5 mg/L picloram produced highest callus biomass among the three varieties. Callus formed on 0.5 mg/L picloram was also much more easily dispersed than callus of all three varieties cultured in the other two MS medium. LV-based medium was generally shown to be poor in inducing callogenic response from leaf explants. Therefore Hi callus sourced from MS medium supplemented with 0.5 mg/L picloram was selected to initiate liquid cell culture of A. annua which can further be explored for production of artemisinin, an anti-malarial compound. Abbreviations: MS – Murashige and Skoog’s salts and vitamins [1]; LV – Litvay medium [2]; BA – 6- benzyladenine; NAA – alpha-napthaleneacetic acid; 2,4-D – 2,4-dichlorophenoxyacetic acid; picloram – 4-amino-3,5,6-trichloropicolinic acid, CH – casein hydrolysate
植物组织培养技术提供了从黄花蒿中生产抗疟疾青蒿素等具有重要医学意义的次生代谢物的潜力。本研究采用叶片外植体在Murashige & Skoog (MS)和Litvay (LV)培养基上添加3种不同添加物,对3个不同品种的黄花蒿(a.a annua) T1、T2和Hi进行愈伤组织诱导。添加0.5 mg/L BA、0.5 mg/L NAA和0.5 g/L酪蛋白水解物(CH)的MS培养基与添加picloram或2,4的MS培养基相比,愈伤组织生物量产量最高。T2品种是该培养基中产量最高的品种。在愈伤组织生物量和脆性方面,富集picloram的MS培养基优于富集2,4的培养基。在添加0.5 mg/L picloram的MS培养基中诱导的Hi品种愈伤组织生物量最高。在0.5 mg/L picloram培养基上形成的愈伤组织也比在其他两种MS培养基上培养的愈伤组织更容易分散。以lv为基础的培养基在诱导叶片外植体的胼胝质形成反应方面表现较差。因此,选择MS培养基中添加0.5 mg/L picloram的Hi愈伤组织,对黄花蒿进行液体细胞培养,进一步探索生产抗疟疾化合物青蒿素。MS - Murashige和Skoog 's盐和维生素[1];LV - Litvay介质[2];BA - 6-苄基腺嘌呤;NAA - -萘乙酸;2,4-d - 2,4-二氯苯氧乙酸;吡咯仑- 4-氨基-3,5,6-三氯吡啶酸CH -酪蛋白水解物
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引用次数: 1
Attitude Towards Science Among Lower Secondary Students in Johor Bahru 新山初中学生对科学的态度
Pub Date : 2018-12-05 DOI: 10.37134/EJSMT.VOL5.2.5.2018
L. S. Yee, Y. Manap
The purpose of the study was to investigate attitude towards science among lower secondary students in Johor Bahru. The attitude in this study included interest, perception towards science and motivation. The study was carried out in Johor Bahru, Johor. The study adopted quantitative descriptive research designs and questionnaires were used in the study. A total of 370 respondents constituted the sample of the study, which included Form 2 lower secondary students from Johor Bahru. Data was analysed to obtain frequency, mean and standard deviation. The results revealed that laboratory activity, curiosity to discovery science and science as a tool to understand the world is able to improve science learning. As a conclusion, interest especially created by laboratory activity, perception of student when they are curios to discovery science and motivation factor when students know that science as a tool to understand the world are able to improve students science learning in lower secondary schools in Johor Bahru. The implication was that school teachers could prepare more laboratory activities in science lesson. They also can stimulate students’ curiosity to discovery science and related science knowledge to the world so that students are interested and motivated to learn science. Future studies on this subject would be beneficial to understand more deeply the relationship between student personal factors with science achievement.
本研究的目的是调查新山初中学生对科学的态度。本研究的态度包括兴趣、对科学的认知和动机。这项研究是在柔佛州的新山进行的。本研究采用定量描述性研究设计,采用问卷调查法。共有370名受访者构成了研究样本,其中包括来自新山的中二低年级学生。对数据进行分析,得到频率、平均值和标准差。研究结果表明,实验室活动、对发现科学的好奇心以及将科学作为认识世界的工具能够促进科学学习。综上所述,实验室活动所产生的兴趣、学生对发现科学的好奇心以及当学生知道科学是了解世界的工具时的动机因素,都能够改善新山初中学生的科学学习。这意味着学校教师可以在科学课上准备更多的实验活动。还能激发学生发现科学和对世界相关科学知识的好奇心,使学生产生学习科学的兴趣和动力。今后对这一课题的研究将有助于更深入地了解学生个人因素与科学成就之间的关系。
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引用次数: 2
Preparation of Multiwall Carbon Nanotubes/Cellulose Nanocomposites Stabilized By 1-Butyl-3-Methyl-Imidazolium (BMIM) - Surfactants 1-丁基-3-甲基咪唑(BMIM)表面活性剂稳定多壁碳纳米管/纤维素纳米复合材料的制备
Pub Date : 2018-12-04 DOI: 10.37134/EJSMT.VOL5.2.3.2018
Rabiaa Abu Azoum Ali, A. Mohamed
Multiwall carbon nanotubes (MWCNTs) ability to improve electrical, optical and mechanical properties of nanocomposites, have attracted great amount of interest for their huge potential in applying them as filler in polymer matrix. However, this application was hindered because of their low dispersion in polymer matrix and tendency to self-associate into macro-scale aggregates.  Recently, diffusion of MWCNTs in cellulose polymer matrix was studied and prepared via latex technology approaches by the addition of 1-butyl-3-methyl-imidazolium (BMIM)-surfactant. The performance of BMIM-surfactants for dispersing MWCNTs in polymer was characterized using a range of techniques including field emission scanning electron microscopy (FESEM), and Thermogravimetric analysis (TGA). Meanwhile, the conductivities of the nanocomposites were also investigated using four-point probe measurements. In this study, MWCNTs were efficiently dispersed in cellulose utilizing 1-butyl-3-methyl imidazolium-dodecyl benzene sulfonate (BMIM-DBS). Interestingly, it was found that BMIM-DBS performs much better than that of the commercially available surfactant sodium dodecyl benzenesulfonate (SDBS), demonstrating the importance of the effect of surfactant counter-ion leading to improved dispersion of MWCNTs in cellulose. This finding will significantly contribute towards the improvement of properties of cellulose for nanocomposite industries.
多壁碳纳米管(MWCNTs)由于能够改善纳米复合材料的电学、光学和力学性能,在聚合物基体中作为填料具有巨大的应用潜力,引起了人们的广泛关注。然而,由于它们在聚合物基体中的分散性低,并且倾向于自缔合成宏观聚集体,因此阻碍了它们的应用。近年来,研究了MWCNTs在纤维素聚合物基体中的扩散,并采用乳液技术方法通过添加1-丁基-3-甲基咪唑(BMIM)表面活性剂制备了MWCNTs。采用场发射扫描电镜(FESEM)和热重分析(TGA)等一系列技术表征了bmi -表面活性剂在聚合物中分散MWCNTs的性能。同时,用四点探针测量了纳米复合材料的电导率。在本研究中,利用1-丁基-3-甲基咪唑-十二烷基苯磺酸盐(BMIM-DBS)将MWCNTs有效地分散在纤维素中。有趣的是,研究发现BMIM-DBS的性能远远优于市售表面活性剂十二烷基苯磺酸钠(SDBS),这表明表面活性剂反离子对改善MWCNTs在纤维素中的分散作用的重要性。这一发现将为纳米复合材料工业中纤维素性能的改善做出重要贡献。
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引用次数: 0
Oxygen Effect in Annealing Process of Aluminium Doped Zinc Oxide Films 掺铝氧化锌薄膜退火过程中的氧效应
Pub Date : 2018-12-04 DOI: 10.37134/EJSMT.VOL5.2.4.2018
B. Astuti, Sugianto, S. N. Mahmudah, P. Marwoto, Didik Ariyanto, E. Wibowo
Al doped ZnO (ZnO:Al) thin films was grown on corning glass substrate using dc magnetron sputtering. ZnO:Al thin film grown with plasma power 40 watt and 500 mTorr argon pressure  for 120 minutes. Film was grown annealed on oxygen atmosphere with different pressure for 20 minutes. The crystalline of ZnO:Al film deposited has an hexagonal structure. The crystallites preferred orientation shift gradually from (002) to (101) direction as the pressure of oxygen increases. Transmittance in the visible regions increases with increasing pressure of oxygen about 81,27%. The optical characterization indicated that the band gap shift toward lower energies with increasing pressure of oxygen.
采用直流磁控溅射技术在康宁玻璃衬底上生长了Al掺杂ZnO (ZnO:Al)薄膜。ZnO:Al薄膜在等离子体功率40瓦,氩气压力500 mTorr条件下生长120分钟。薄膜在不同压力的氧气气氛中生长退火20分钟。制备的ZnO:Al薄膜的结晶具有六方结构。随着氧压力的增加,晶体择优取向逐渐由(002)向(101)方向转变。可见光区的透光率随氧气压力的增加而增加约81,27%。光学表征表明,随着氧压力的增加,带隙向低能方向移动。
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引用次数: 0
Nurturing Habits of Mind (HOM) Through Thinking Based Learning (TBL) in Doing Science Technology, Engineering and Mathematics (STEM) Project 通过基于思维的学习(TBL)在科学、技术、工程和数学(STEM)项目中培养思维习惯(HOM)
Pub Date : 2018-12-03 DOI: 10.37134/EJSMT.VOL5.2.2.2018
H. Hashim, Mohd Norawi Ali, M. Samsudin
Habits of mind (HOM) is one of the kind response to those questions and problems whereby the answers to which are not immediately known which can attribute to effective problem solvers and critical thinking development. However, the emphasis on this aspect is still low especially among science educators as well as secondary science students which in the long run will retard the production of knowledge worker who are able to behave when confronted with life’s problems. The study addressed the integration of Thinking Based Learning (TBL) and the 6E Instructional Model as a teaching approach in nurturing habits of mind among secondary students while doing their Science, Technology, Engineering and Mathematics (STEM) project. The study involved 30 students from a Lower Secondary School in a sub urban school in Kota Bharu, Kelantan. Students were given a STEM Raft Module integrating TBL and 6E Instructional Model for five weeks to come out with a model of a raft based on science concepts using material available in their community. Data of the study were captured from students’ documentation analysis, classroom observation using camera and video cam recording, and interview. The findings showed that TBL and 6E Instructional Model can nurture habits of mind among secondary students while doing their STEM project. Seven characteristics of habits of mind have been identified; (1) questioning and problem solving, (2) listening with understanding and empathy, (3) thinking about your thinking, (4) thinking flexibility, (5) applying past knowledge to novel situations, (6) creating, imagining and innovating and (7) thinking independently.  Keywords: Habits of mind, Thinking Based Learning, the 6E Instructional Model, STEM project
思维习惯(HOM)是对那些不能立即知道答案的问题和问题的一种反应,它可以归因于有效的问题解决者和批判性思维的发展。然而,对这方面的重视程度仍然很低,特别是在科学教育者和中学理科学生中,从长远来看,这将阻碍知识工作者的产生,他们在面对生活问题时能够表现出来。该研究探讨了基于思维的学习(TBL)和6E教学模式的整合,作为一种在中学生进行科学、技术、工程和数学(STEM)项目时培养思维习惯的教学方法。这项研究涉及吉兰丹州哥打巴鲁一所郊区学校的一所初中的30名学生。学生们将在五周的时间里学习STEM木筏模块,该模块整合了TBL和6E教学模型,并利用社区提供的材料,根据科学概念制作木筏模型。本研究的数据采自学生的文献分析、使用相机和视频记录的课堂观察和访谈。研究结果表明,TBL和6E教学模式能够培养中学生在STEM项目中的思维习惯。人们已经确定了思维习惯的七个特征;(1)提问和解决问题,(2)用理解和同理心倾听,(3)思考自己的想法,(4)思维灵活性,(5)将过去的知识应用于新的情况,(6)创造、想象和创新,(7)独立思考。关键词:思维习惯,基于思维的学习,6E教学模式,STEM项目
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引用次数: 4
Medicine Card as Innovation Learning in Periodic Table of Elements for Pharmaceutical Vocational Students 药卡作为药学高职学生元素周期表的创新学习
Pub Date : 2018-12-03 DOI: 10.37134/EJSMT.VOL5.2.1.2018
Zulfah, Eka Statistika, Eti Sukriani
The innovation in this instruction was the use of medicine card as a learning media of periodic table of elements. This research aimed to know the process of learning conducted by using medicine card and to know the students' motivation and learning outcomes as the result of the implementation of medicine card in the classroom activity. This research used a quantitative descriptive method. The implementation of the media was conducted in four-fold meetings in Health Vocational School of Sadewa, Yogyakarta, Indonesia. A SWOT analysis was conducted at the beginning of the learning innovation and was followed by the observation and the preparation of material and media. The class activity began with explanations of medicine card games, group divisions, game execution, and confirmation. The final step was evaluated using a questionnaire of motivation and post-test problems. The result of the questionnaire indicated that the students’ motivation was in the good criterion and the learning outcomes of students showed that they had an enough criterion.
这一教学的创新之处在于将药卡作为元素周期表的学习媒介。本研究旨在了解使用药卡进行学习的过程,了解在课堂活动中实施药卡后学生的学习动机和学习成果。本研究采用定量描述方法。在印度尼西亚日惹Sadewa卫生职业学校举行的四次会议上实施了媒体。在学习创新之初进行SWOT分析,然后进行观察,准备材料和媒介。课堂活动从医学卡牌游戏讲解、小组划分、游戏执行、确认开始。最后一步是使用动机问卷和测试后问题进行评估。问卷调查结果显示,学生的学习动机处于良好的标准,学生的学习成果显示他们有一个足够的标准。
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引用次数: 0
Chromatographic Fingerprint analysis for Determining the Presence of Vitexin and Isovitexin on Seven Varieties of Ficus Deltoidea 7个三角榕品种中牡荆素和异牡荆素的色谱指纹图谱分析
Pub Date : 2018-06-11 DOI: 10.37134/ejsmt.vol5.1.6.2018
Z. Abdullah, N. A. Ali, M. Jamil, N. Mat, A. Ali, N. Awang, Wan Ainor Syahdah Wan Hassan, L. S. Kiong, Mohd Hafidz Abdullah, Norfaizura Azmi
Ficus deltoidea Jack or locally known as mas cotek from the family of Moraceae has been acknowledged for its occurrence of several varieties based on the variations of leaf morphology, namely F. deltoidea var. angustifolia (Miq.) Corner, F. deltoidea var. bilobata Corner, F. deltoidea var. kunstleri (King) Corner, F. deltoidea var. lutescens (Desf.) Corner, F. deltoidea var. trengganuensis Corner, F. deltoidea var. motleyana and F. deltoidea var. deltoidea Jack.  In this work, we report on the chemical profiles using chromatographic techniques on the seven varieties of F. deltoidea by analysing the presence of the bioactive compounds, vitexin and isovitexin and characterising the other major peaks in the chromatograms. Observation on the HPLC chromatogram showed the presence of both compounds in all samples except FDv. K (vitexin only) and FDv. I.  Other peaks showed a characteristic of flavonoids based on the UV spectra pattern with UV maxima at 270 and 338 nm except for peaks 6, 7 and 8 with UV maxima at 294, 319 and 210, respectively.  Both reference compounds were presence in varies concentration in all samples analysed.
来自桑科(Moraceae)的Ficus deltoidea Jack或在当地被称为mas cotek,因其基于叶片形态变化的几个品种而得到认可,即F. deltoidea var. angustifolia (Miq.)。角,F. deltoidea var. bilobata角,F. deltoidea var. kunstleri (King)角,F. deltoidea var. lutescens (Desf.)角、角、角、角、角、角、角、角、角、角、角本文利用色谱分析技术,分析了7个品种的牡荆素和异牡荆素的活性成分,并对其主要峰进行了表征。HPLC分析结果表明,除FDv外,其余样品均存在这两种化合物。K(仅限牡荆素)和FDv。1 .除峰6、峰7和峰8分别在294、319和210 nm处出现紫外峰值外,其余峰均在270和338 nm处表现出黄酮类化合物的特征。两种标准化合物在所有分析样品中均以不同浓度存在。
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引用次数: 2
Spatial Assessment of Water Quality Patterns using Environmetric Techniques: A Case Study in Muda River Basin (Malaysia) 基于环境技术的水质格局空间评价——以马来西亚慕达河流域为例
Pub Date : 2018-06-08 DOI: 10.37134/ejsmt.vol5.1.5.2018
S. Azhar
River pollution impact human health, environment and the sustainable development. This study was conducted to identify spatial patterns and the main parameters affecting the water pollution within nine monitoring stations in the Muda River basin (Malaysia) over a 16-year database (1998–2013). Environmetric techniques were applied to the dataset. These combined Cluster Analysis, Discriminant Analysis, and Multiple Linear Regression. The Cluster Analysis showed that the monitoring stations divided into two separate groups based on similarities features of water quality while Discriminant Analysis validated these groups. Furthermore, the Multiple Linear Regression analysis showed that the significant parameters contributing to variability the Water Quality Index was biological oxygen demand, chemical oxygen demand and ammonia nitrogen. This was due to the point-source pollution, particularly from rubber factory. Therefore, the results provided information to support future water pollution control strategies.
河流污染影响人类健康、环境和可持续发展。本研究对马来西亚木达河流域9个监测站的16年数据(1998-2013)进行了空间格局和主要污染参数的识别。环境测量技术应用于数据集。这些方法结合了聚类分析、判别分析和多元线性回归。聚类分析结果表明,监测站根据水质特征的相似性划分为两组,判别分析对两组进行了验证。多元线性回归分析表明,生物需氧量、化学需氧量和氨氮是影响水质指数变异的显著参数。这是由于点源污染,特别是来自橡胶厂。因此,研究结果为支持未来的水污染控制策略提供了信息。
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引用次数: 1
Multimedia of Chemistry Enrichment in Colloidal Systems for Vocational High School Students 高职学生胶体系统化学富集的多媒体教学
Pub Date : 2018-06-08 DOI: 10.37134/ejsmt.vol5.1.4.2018
M. K. Nais, E. D. Siswani
This research was conducted to develop the multimedia of colloidal systems as an enrichment media for vocational high school students in chemistry class and to know the quality of the multimedia. This was a research and development study. Developmental procedures used in this research were adopted from Borg and Gall development procedures consisting of six phases including: collecting information, planning, developing the preliminary form of the product, validating and revising the product, assessing the product, and revising final product. The products were validated by one content expert and one media expert and reviewed by three peer reviewers. The advises from the experts and the peer reviewers were used to improve the initial product. The assessment of the product was carried out by five reviewers based on six aspects of the criteria, included: content feasibility, language, visual-display of media, audio-display of media, media programming, and expediency of the media. The product assessment data were analyzed by categorizing the score into ideal rating category. In general, the multimedia assessment score was included in the category of very good category. Analysis on each aspect shows that content feasibility, media programming, and expediency of the media aspects was included in the very good category, while language, visual-display of media, audio-display of media aspects included in the good category. These results indicatated that the products were ideal as an enrichment media of colloidal systems for vocational high school students.
本研究旨在开发多媒体胶体系统作为中职学生化学课堂的富集介质,并了解多媒体的质量。这是一项研究和发展研究。本研究中使用的开发程序采用Borg和Gall开发程序,包括六个阶段:收集信息,计划,开发产品的初步形式,验证和修改产品,评估产品,修改最终产品。产品由一名内容专家和一名媒体专家进行验证,并由三名同行评审员进行评审。专家和同行审稿人的建议被用来改进最初的产品。五名评审员根据内容可行性、语言、媒体视觉展示、媒体听觉展示、媒体程序化、媒体便利性六个方面的标准对产品进行评估。对产品评价数据进行分析,将得分分为理想评定类。总的来说,多媒体评估得分被列入非常好的类别。对各方面的分析表明,媒体的内容可行性、媒体的程序化、媒体的权宜之计等方面属于很好的范畴,语言、媒体的视觉展示、媒体的听觉展示等方面属于很好的范畴。这些结果表明,该产物是理想的胶体体系富集介质,适用于中职高中生。
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引用次数: 0
Exploration of Procedural Knowledge of Bilingual Students in Chemical Equilibrium Problem Solving 双语学生化学平衡问题解决过程性知识的探索
Pub Date : 2018-06-07 DOI: 10.37134/ejsmt.vol5.1.3.2018
B. Y. Sawuwu, C. F. Partana, Hairida -
This research was conducted to explore how procedural knowledge of bilingual students in chemical equilibrium problem-solving skills. This research was a descriptive study of 31 bilingual private high school students. Chemical equilibrium problem solving skills were collected by three problems, while the procedural knowledge was collected by analysis of student answers, self-assessment metacognition questionnaire, and the interview with students and their chemistry teacher. Data collected was analyzed by a phenomenological reduction method. The results of students’ procedural knowledge were categorized into assumption determination (54,84%), strategy development (22,58%), chemical equation (41,94%), factors affecting the equation (61,29%), using the other knowledge (29,03%), quantitative analysis (38,71%), and alternative strategy (25,81%). According to the operator in each framework answer, the result of students’ answers of chemical equilibrium problem-solving skills was classified into phenomenological and formalism answers. In phenomenological answers, students began their framework from the chemical equation to factors affecting the equilibrium shift. In the formalism answer, the students began their framework from the information given in the problems and related it to the stoichiometry and alternative ways to find the final states (goals). In this study was also found some students’ errors in chemical language and chemical equilibrium conceptions.
本研究旨在探讨程序性知识对双语学生化学平衡问题解决能力的影响。本研究是对31名双语私立高中学生进行描述性研究。化学平衡问题解决技能通过三个问题收集,程序性知识通过分析学生答案、自我评估元认知问卷、对学生及其化学老师的访谈收集。收集的数据用现象学还原法进行分析。学生程序性知识的结果分为假设确定(54,84%)、策略制定(22,58%)、化学方程(41,94%)、影响方程的因素(61,29%)、使用其他知识(29,03%)、定量分析(38,71%)和备选策略(25,81%)。根据每个框架答案中的算子,将学生化学平衡问题解决技能的答案分为现象学和形式主义两类。在现象学的回答中,学生从化学方程式到影响平衡转移的因素开始他们的框架。在形式主义的答案中,学生们从问题中给出的信息开始他们的框架,并将其与化学计量和找到最终状态(目标)的替代方法联系起来。在本研究中还发现了一些学生在化学语言和化学平衡概念上的错误。
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引用次数: 1
期刊
EDUCATUM Journal of Science, Mathematics and Technology
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