Pub Date : 1996-01-01DOI: 10.3109/10623329609024684
F. Salazar
The importance of nitric oxide in the acute and long-term regulation of arterial pressure and renal function is supported by the results obtained in many studies. Reports described in this brief review provide evidences that nitric oxide plays an important role in regulating the urinary sodium and water excretion in basal conditions and during increments in arterial pressure and extracellular volume. Data showing an important interaction between nitric oxide and angiotensin II or prostaglandins in the regulation of the renal excretory function is also provided. These studies support the concept that the development of sodium sensitive hypertension could be secondary to a deficient nitric oxide production.
{"title":"Nitric Oxide and Renal Regulation of Sodium Excretion","authors":"F. Salazar","doi":"10.3109/10623329609024684","DOIUrl":"https://doi.org/10.3109/10623329609024684","url":null,"abstract":"The importance of nitric oxide in the acute and long-term regulation of arterial pressure and renal function is supported by the results obtained in many studies. Reports described in this brief review provide evidences that nitric oxide plays an important role in regulating the urinary sodium and water excretion in basal conditions and during increments in arterial pressure and extracellular volume. Data showing an important interaction between nitric oxide and angiotensin II or prostaglandins in the regulation of the renal excretory function is also provided. These studies support the concept that the development of sodium sensitive hypertension could be secondary to a deficient nitric oxide production.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"57 1","pages":"77-83"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74630401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024705
M. Nobles, N. Abbott
For most studies of cultured endothelium, cells are grown on tissue culture plastic. However, for microscopic studies involving the use of fluorescent dyes, the autofluorescence of the plastic can be a problem. Glass is a good alternative to plastic in this case, but standard methods for coating tissue culture plastic to provide an adhesive substrate do not work well on glass. We here report studies on the morphology and growth characteristics of rat brain microvascular endothelial cells growing on glass treated to improve adhesion by changing the collagen-to-glass binding properties and finally coated with collagen. Three methods were tested, using primary cultures of rat brain endothelial cells, and an immortalized rat brain endothelial cell line (RBE4). Cells grew poorly on an extracellular matrix laid down by prior culture of rat type I astrocytes. Cells grew equally well on glass coated with poly-L-lysine or its succinylated derivative. However, a further method gave an even more stable and confluent...
{"title":"Adhesion and Growth of Brain Microvascular Endothelial Cells on Treated Glass","authors":"M. Nobles, N. Abbott","doi":"10.3109/10623329609024705","DOIUrl":"https://doi.org/10.3109/10623329609024705","url":null,"abstract":"For most studies of cultured endothelium, cells are grown on tissue culture plastic. However, for microscopic studies involving the use of fluorescent dyes, the autofluorescence of the plastic can be a problem. Glass is a good alternative to plastic in this case, but standard methods for coating tissue culture plastic to provide an adhesive substrate do not work well on glass. We here report studies on the morphology and growth characteristics of rat brain microvascular endothelial cells growing on glass treated to improve adhesion by changing the collagen-to-glass binding properties and finally coated with collagen. Three methods were tested, using primary cultures of rat brain endothelial cells, and an immortalized rat brain endothelial cell line (RBE4). Cells grew poorly on an extracellular matrix laid down by prior culture of rat type I astrocytes. Cells grew equally well on glass coated with poly-L-lysine or its succinylated derivative. However, a further method gave an even more stable and confluent...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"28 1","pages":"297-307"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74804885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024698
Jane H.-C. Lin, Y. Kobari, Yi Zhu, M. Stemerman, K. Pritchard
Human umbilical vein endothelial cells (EC) metabolize arachidonic acid (AA) through three major pathways—cyclooxygenase, lipoxygenase and cytochrome P450 (P450) isozymes. Previously, we have shown that pathophysiological concentrations of native low density lipoprotein (n-LDL) increase EC P450-dependent epoxyeicosatrienoic acid (EET) production. The present study was designed to identify putative P450 isozymes involved in EC epoxidation of AA. Reverse transcription—polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) were employed to detect P450 2 family cDNA from EC mRNA. Degenerate primers complimenting 2 homologous regions from 6 different P450 2 families were designed to capture a 440 bp cDNA fragment corresponding to the heme binding region of P450 2 isozymes. RT-PCR of EC total RNA with these primers amplified a 440 bp fragment. After gel purification, the fragment was cloned, sequenced and found to share a high degree of identity with human liver P450 2C8 and 2C9. New pri...
{"title":"Human umbilical vein endothelial cells express P450 2C8 mRNA : cloning of endothelial P450 epoxygenase","authors":"Jane H.-C. Lin, Y. Kobari, Yi Zhu, M. Stemerman, K. Pritchard","doi":"10.3109/10623329609024698","DOIUrl":"https://doi.org/10.3109/10623329609024698","url":null,"abstract":"Human umbilical vein endothelial cells (EC) metabolize arachidonic acid (AA) through three major pathways—cyclooxygenase, lipoxygenase and cytochrome P450 (P450) isozymes. Previously, we have shown that pathophysiological concentrations of native low density lipoprotein (n-LDL) increase EC P450-dependent epoxyeicosatrienoic acid (EET) production. The present study was designed to identify putative P450 isozymes involved in EC epoxidation of AA. Reverse transcription—polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) were employed to detect P450 2 family cDNA from EC mRNA. Degenerate primers complimenting 2 homologous regions from 6 different P450 2 families were designed to capture a 440 bp cDNA fragment corresponding to the heme binding region of P450 2 isozymes. RT-PCR of EC total RNA with these primers amplified a 440 bp fragment. After gel purification, the fragment was cloned, sequenced and found to share a high degree of identity with human liver P450 2C8 and 2C9. New pri...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"29 1","pages":"219-229"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82158635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024688
G. Wiemer, B. Pierchala, Š. Mesároš, B. Scholkens, T. Malinski
Stimulation of endothelial nitric oxide production by bradykinin or the angiotensin converting enzyme inhibitor “ramiprilat” has been indirectly assessed in terms of intracellular cyclic GMP accumulation. However, direct measurement of endothelial nitric oxide synthesis and release has not been analyzed. Using a selective porphyrinic microsensor, nitric oxide release was detected from single primary cultured bovine aortic endothelial cells. Maximal nitric oxide release of 540 ± 38 or 122 ± 10 n mol/L was achieved by bradykinin at 5 × 10−8 mol/L or by ramiprilat at 10−7 mol/L respectively. The time course of nitric oxide release by bradykinin showed a rapid initial production rate (22.5 ± 1.6 nmol/L/sec, 4.2% increase per second) and was transient within 15 min (decay rate 0.60 ± 0.05 nmol/L/sec). In contrast, the initial production rate of nitric oxide release by ramiprilat was slow (0.34 ± 0.03 nmol/L/sec, 0.28% increase per second) and reached a plateau level after 6 min, which remained stable for at le...
{"title":"Direct Measurement of Nitric Oxide Release from Cultured Endothelial Cells Stimulated by Bradykinin or Ramiprilat","authors":"G. Wiemer, B. Pierchala, Š. Mesároš, B. Scholkens, T. Malinski","doi":"10.3109/10623329609024688","DOIUrl":"https://doi.org/10.3109/10623329609024688","url":null,"abstract":"Stimulation of endothelial nitric oxide production by bradykinin or the angiotensin converting enzyme inhibitor “ramiprilat” has been indirectly assessed in terms of intracellular cyclic GMP accumulation. However, direct measurement of endothelial nitric oxide synthesis and release has not been analyzed. Using a selective porphyrinic microsensor, nitric oxide release was detected from single primary cultured bovine aortic endothelial cells. Maximal nitric oxide release of 540 ± 38 or 122 ± 10 n mol/L was achieved by bradykinin at 5 × 10−8 mol/L or by ramiprilat at 10−7 mol/L respectively. The time course of nitric oxide release by bradykinin showed a rapid initial production rate (22.5 ± 1.6 nmol/L/sec, 4.2% increase per second) and was transient within 15 min (decay rate 0.60 ± 0.05 nmol/L/sec). In contrast, the initial production rate of nitric oxide release by ramiprilat was slow (0.34 ± 0.03 nmol/L/sec, 0.28% increase per second) and reached a plateau level after 6 min, which remained stable for at le...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"258 1","pages":"119-125"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77092213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024677
G. Donnarumma, F. Brancaccio, Gabriella Cipollaro Del'ero, A. Folgore, A. Marcatili, M. Galdiero
Gram-negative (Salmonella typhimurium porins and lipopolysaccharide-R), and Gram-positive bacterial components (lipoteichoic acid, muramic acid, muramyl-dipeptide, adjuvant peptide, protein A, toxic shock syndrome toxin-1, α-hemolysin) were tested for their ability to stimulate both the release of Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF), soluble Intercellular Adhesion Molecule-1 (ICAM-1) and soluble E-selectin from human endothelial cells, and the surface expression of the adhesion molecules. Salmonella typhimurium porins and lipopolysaccharide-R (LPS-R) were able to induce the release of GM-CSF, sE-selectin and sICAM-1 in a dose dependent fashion. The greatest release of these factors was obtained using pork at a concentration of 5μg/ml and LPS-R at a concentration of 1μg/ml. The kinetics of release showed that LPS-R was able to stimulate the production of these factors earlier than porins but at a lower rate. Porins and LPS-R was also able to up-regulate the surface expression of E-selec...
革兰氏阴性细菌成分(鼠伤寒沙门氏菌孔蛋白和脂多糖- r)和革兰氏阳性细菌成分(脂壁酸、乳杆菌酸、乳杆菌二肽、辅助肽、蛋白A、中毒性休克综合征毒素-1、α-溶血素)刺激人内皮细胞释放粒细胞-单核细胞集落刺激因子(GM-CSF)、可溶性细胞间粘附分子-1 (ICAM-1)和可溶性e-选择素的能力进行了测试。以及粘附分子的表面表达。鼠伤寒沙门菌孔蛋白和脂多糖- r (LPS-R)能够诱导GM-CSF、sE-selectin和sICAM-1的释放，并呈剂量依赖性。5μg/ml的猪肉和1μg/ml的LPS-R对这些因子的释放效果最好。释放动力学表明，LPS-R能够比孔蛋白更早地刺激这些因子的产生，但速率较低。孔蛋白和LPS-R也能上调E-selec的表面表达。
{"title":"Release of GM-CSF, sE-Selectin, and SICAM-1 by Human Vascular Endothelium Stimulated with Gram-Negative and Gram-Positive Bacterial Components","authors":"G. Donnarumma, F. Brancaccio, Gabriella Cipollaro Del'ero, A. Folgore, A. Marcatili, M. Galdiero","doi":"10.3109/10623329609024677","DOIUrl":"https://doi.org/10.3109/10623329609024677","url":null,"abstract":"Gram-negative (Salmonella typhimurium porins and lipopolysaccharide-R), and Gram-positive bacterial components (lipoteichoic acid, muramic acid, muramyl-dipeptide, adjuvant peptide, protein A, toxic shock syndrome toxin-1, α-hemolysin) were tested for their ability to stimulate both the release of Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF), soluble Intercellular Adhesion Molecule-1 (ICAM-1) and soluble E-selectin from human endothelial cells, and the surface expression of the adhesion molecules. Salmonella typhimurium porins and lipopolysaccharide-R (LPS-R) were able to induce the release of GM-CSF, sE-selectin and sICAM-1 in a dose dependent fashion. The greatest release of these factors was obtained using pork at a concentration of 5μg/ml and LPS-R at a concentration of 1μg/ml. The kinetics of release showed that LPS-R was able to stimulate the production of these factors earlier than porins but at a lower rate. Porins and LPS-R was also able to up-regulate the surface expression of E-selec...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"294 1","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90801098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024695
N. R. Sharma, M. Davis
Substance P (SP) activates an outward K+ current, membrane hyperpolarization and increases cytosolic Ca2+ ([Ca2+],) in porcine coronary artery endothelial cells (PCAECs). We tested the importance of Ca2+, CAMP, cGMP, arachidonic acid and G protein-linked pathways in modulating whole-cell K+ currents activated by SP. Current or membrane potential and [Ca2+], were measured simultaneously in single cells in response to SP (10 nM), using the perforated patch technique and fura-2 microfluorimetry. Partial block of Kcachannels by D-tubocurarine resulted in a significant reduction in SP-induced membrane hyperpolarization and the plateau phase of the cell [Ca2+]i response. In PCAECs preloaded with BAPTA, SP failed to elevate [Ca2+]i or activate outward current. In Ca2+-free bath solution, SP induced transient elevations in [Ca2+]i and outward current. With SP still present, addition of 1 mM Ca2+ to the bath after [Ca2+]i returned to resting levels elevated [Ca2+]i and elicited outward current. Acute application o...
P物质(SP)在猪冠状动脉内皮细胞(PCAECs)中激活向外K+电流，膜超极化并增加胞质Ca2+ ([Ca2+]，)。我们测试了Ca2+， CAMP, cGMP，花生四烯酸和G蛋白连接通路在SP激活的全细胞K+电流调节中的重要性。使用穿孔贴片技术和fura-2微荧光法，在响应SP (10 nM)的单细胞中同时测量电流或膜电位和[Ca2+]。d -管curarine部分阻断Kcachannels导致sp诱导的膜超极化和细胞[Ca2+]i反应的平台期显著减少。在预加载BAPTA的pcaec中，SP不能升高[Ca2+]i或激活外向电流。在无Ca2+溶液中，SP诱导[Ca2+]i和向外电流的瞬时升高。在SP仍然存在的情况下，在[Ca2+]i恢复到静息水平后，向浴液中添加1 mM Ca2+可提高[Ca2+]i并引发向外电流。急性应用…
{"title":"Modulation of Substance P-Induced K+ Current in Coronary Endothelium","authors":"N. R. Sharma, M. Davis","doi":"10.3109/10623329609024695","DOIUrl":"https://doi.org/10.3109/10623329609024695","url":null,"abstract":"Substance P (SP) activates an outward K+ current, membrane hyperpolarization and increases cytosolic Ca2+ ([Ca2+],) in porcine coronary artery endothelial cells (PCAECs). We tested the importance of Ca2+, CAMP, cGMP, arachidonic acid and G protein-linked pathways in modulating whole-cell K+ currents activated by SP. Current or membrane potential and [Ca2+], were measured simultaneously in single cells in response to SP (10 nM), using the perforated patch technique and fura-2 microfluorimetry. Partial block of Kcachannels by D-tubocurarine resulted in a significant reduction in SP-induced membrane hyperpolarization and the plateau phase of the cell [Ca2+]i response. In PCAECs preloaded with BAPTA, SP failed to elevate [Ca2+]i or activate outward current. In Ca2+-free bath solution, SP induced transient elevations in [Ca2+]i and outward current. With SP still present, addition of 1 mM Ca2+ to the bath after [Ca2+]i returned to resting levels elevated [Ca2+]i and elicited outward current. Acute application o...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"29 1","pages":"189-197"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86105042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024678
Chi-Ming Wei, V. Miller, H. Schaff, J. Burnett
We investigated the actions of the two structurally similar but genetically distinct endothelium-derived vasoactive natriuretic peptides, C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP), upon isolated human internal mammary arteries and saphenous veins. Based upon previous studies in canine arteries and veins, we hypothesized that CNP would selectively cause relaxation of human veins while ANP would be selective for human arteries. Rings with and without endothelium of human internal mammary arteries and saphenous veins from patients under going coronary artery bypass grafting were suspended for measurement of isometric force in an organ chamber. CNP caused significant concentration-dependent relaxations in saphenous veins with and without endothelium contracted with phenylephrine (10−6 M). In marked contrast, ANP caused no relaxation in saphenous veins either with or without endothelium. In rings of internal mammary arteries, ANP caused significant concentration-dependent relaxation...
{"title":"Specificity of C-type and atrial natriuretic peptides in human blood vessels","authors":"Chi-Ming Wei, V. Miller, H. Schaff, J. Burnett","doi":"10.3109/10623329609024678","DOIUrl":"https://doi.org/10.3109/10623329609024678","url":null,"abstract":"We investigated the actions of the two structurally similar but genetically distinct endothelium-derived vasoactive natriuretic peptides, C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP), upon isolated human internal mammary arteries and saphenous veins. Based upon previous studies in canine arteries and veins, we hypothesized that CNP would selectively cause relaxation of human veins while ANP would be selective for human arteries. Rings with and without endothelium of human internal mammary arteries and saphenous veins from patients under going coronary artery bypass grafting were suspended for measurement of isometric force in an organ chamber. CNP caused significant concentration-dependent relaxations in saphenous veins with and without endothelium contracted with phenylephrine (10−6 M). In marked contrast, ANP caused no relaxation in saphenous veins either with or without endothelium. In rings of internal mammary arteries, ANP caused significant concentration-dependent relaxation...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"25 1","pages":"23-28"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78577272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024691
Zheng Yuan, D. Atchison, M. Johnston
We studied the effects of endothelin family peptides on the pumping responses of lymphatic vessels, the receptors involved and whether vessel-derived endothelin played a role in the myogenic response to changes in transmural pressure. Postnodal bovine mesenteric lymphatics were suspended in an organ bath preparation with both inflow and outflow ends cannulated. Input to the ducts was provided from a reservoir filled with Krebs solution. With a 6 cm H2O fixed transmural pressure applied to the vessels to initiate spontaneous contractions, ET-1, ET-2 and ET-3 (tested between 10−10 and 10−8PM) depressed lymphatic pumping in a concentration-dependent fashion with the order of potency being ET-1 = ET-2 > ET-3. The concentrations of ET-1 and ET-2 that depressed pumping 50% were 0.96 × 10−9M and 0.95 × 10−9M respectively. Similarly, when transmural pressures were varied in 2 cm H2O increments between 0 and 14 cm H2O, ET-1 at 10−9 and 10−8M significantly depressed fluid pumping at the majority of distending press...
{"title":"Endothelin Modulation of Pumping Activity in Bovine Mesenteric Lymphatic Vessels","authors":"Zheng Yuan, D. Atchison, M. Johnston","doi":"10.3109/10623329609024691","DOIUrl":"https://doi.org/10.3109/10623329609024691","url":null,"abstract":"We studied the effects of endothelin family peptides on the pumping responses of lymphatic vessels, the receptors involved and whether vessel-derived endothelin played a role in the myogenic response to changes in transmural pressure. Postnodal bovine mesenteric lymphatics were suspended in an organ bath preparation with both inflow and outflow ends cannulated. Input to the ducts was provided from a reservoir filled with Krebs solution. With a 6 cm H2O fixed transmural pressure applied to the vessels to initiate spontaneous contractions, ET-1, ET-2 and ET-3 (tested between 10−10 and 10−8PM) depressed lymphatic pumping in a concentration-dependent fashion with the order of potency being ET-1 = ET-2 > ET-3. The concentrations of ET-1 and ET-2 that depressed pumping 50% were 0.96 × 10−9M and 0.95 × 10−9M respectively. Similarly, when transmural pressures were varied in 2 cm H2O increments between 0 and 14 cm H2O, ET-1 at 10−9 and 10−8M significantly depressed fluid pumping at the majority of distending press...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"28 1","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82872884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024693
Rosella Sbarbati-Delguerra, E. Fommei, F. Pecori, P. Gazzetti, M. G. Chicca
We studied the influence of hypertensive (HT) serum on human umbilical vein endothelial cell (HUVEC) and on human umbilical artery smooth muscle cell (HUASMC) proliferation. We also tested the possible influence of the HT serum on vasoconstrictor substances released by HUVECs.Pools of HT and normotensive (NT) sera were collected and used to supplement cell culture medium. Both HUVECs and HUASMCs were conditioned with culture media supplemented with either HT or NT sera. After conditioning, both types of cells were tested for proliferative capacity. Under the same experimental conditions the HUVEC conditioned medium was assayed for angiotensin II (AII) and endothelin 1 (ET,). All and ET, were also assayed after acute exposure to HT serum of HUVEC preconditioned with NT serum.Our results showed that HUVEC and HUASMC conditioned with HT serum substantially enhanced cell proliferation. Preconfluent HUVECs conditioned with HT serum showed higher levels of AZZ and lower levels of ET1 compared with the controls ...
{"title":"Hypertensive Serum Enhances Human Vascular Cell Growth and Modulates Endothelial Angiotensin II and Endothelin in Culture","authors":"Rosella Sbarbati-Delguerra, E. Fommei, F. Pecori, P. Gazzetti, M. G. Chicca","doi":"10.3109/10623329609024693","DOIUrl":"https://doi.org/10.3109/10623329609024693","url":null,"abstract":"We studied the influence of hypertensive (HT) serum on human umbilical vein endothelial cell (HUVEC) and on human umbilical artery smooth muscle cell (HUASMC) proliferation. We also tested the possible influence of the HT serum on vasoconstrictor substances released by HUVECs.Pools of HT and normotensive (NT) sera were collected and used to supplement cell culture medium. Both HUVECs and HUASMCs were conditioned with culture media supplemented with either HT or NT sera. After conditioning, both types of cells were tested for proliferative capacity. Under the same experimental conditions the HUVEC conditioned medium was assayed for angiotensin II (AII) and endothelin 1 (ET,). All and ET, were also assayed after acute exposure to HT serum of HUVEC preconditioned with NT serum.Our results showed that HUVEC and HUASMC conditioned with HT serum substantially enhanced cell proliferation. Preconfluent HUVECs conditioned with HT serum showed higher levels of AZZ and lower levels of ET1 compared with the controls ...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"47 1","pages":"171-176"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89178812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1996-01-01DOI: 10.3109/10623329609024683
P. Milner, A. Loesch, G. Burnstock
There is evidence that long-term changes in the innervation of vascular smooth muscle alters the flow-stimulated release of vasoactive substances from the endothelium. To investigate the endothelial content of these substances following chronic sensory denervation, the distribution of endotheli-1 (ET-l), substance P (SP) and arginine vasopressin (AVP) in mature rat pulmonary artery endothelial cells was examined after neonatal treatment with the selective sensory neurotoxin, capsaicin. After immunolabelling with antibodies to the vasoactive peptides, Hautchen preparations of sheets of endothelial cells were observed and the percentage of immunopositive cells recorded. Preparations from capsaicin-treated rats displayed significantly more cells immunopositive to SP than controls (36% ± 3, n = 4 versus 9% ± 4, n = 4, P < 0.01) and less cells immunopositive to ET-1 (36% ± 4, n = 4 versus 56% ± 3, n = 4, P < 0.01). The distribution of vasopressin-immunopositive cells was unchanged by capsaicin treatment (10% ±...
有证据表明，血管平滑肌神经支配的长期变化改变了血流刺激的血管活性物质从内皮细胞释放。为了研究慢性感觉神经支配后内皮细胞中这些物质的含量，我们用选择性感觉神经毒素辣椒素处理新生大鼠肺动脉内皮细胞后，观察了内皮素-1 (et -1)、P物质(SP)和精氨酸加压素(AVP)在新生大鼠肺动脉内皮细胞中的分布。用血管活性肽抗体进行免疫标记后，观察内皮细胞片的Hautchen制备，记录免疫阳性细胞的百分比。辣椒素处理大鼠制备物中对SP免疫阳性的细胞数量显著高于对照组(36%±3,n = 4比9%±4,n = 4, P < 0.01)，对ET-1免疫阳性的细胞数量显著低于对照组(36%±4,n = 4比56%±3,n = 4, P < 0.01)。辣椒素处理后抗利尿激素免疫阳性细胞的分布(10%±…
{"title":"Neonatal Sensory Denenration Affects the Expression of Endothelial Peptides in the Adult Rat Pulmonary Artery: More Cells Contain Substance P and Less Contain Endothelin","authors":"P. Milner, A. Loesch, G. Burnstock","doi":"10.3109/10623329609024683","DOIUrl":"https://doi.org/10.3109/10623329609024683","url":null,"abstract":"There is evidence that long-term changes in the innervation of vascular smooth muscle alters the flow-stimulated release of vasoactive substances from the endothelium. To investigate the endothelial content of these substances following chronic sensory denervation, the distribution of endotheli-1 (ET-l), substance P (SP) and arginine vasopressin (AVP) in mature rat pulmonary artery endothelial cells was examined after neonatal treatment with the selective sensory neurotoxin, capsaicin. After immunolabelling with antibodies to the vasoactive peptides, Hautchen preparations of sheets of endothelial cells were observed and the percentage of immunopositive cells recorded. Preparations from capsaicin-treated rats displayed significantly more cells immunopositive to SP than controls (36% ± 3, n = 4 versus 9% ± 4, n = 4, P < 0.01) and less cells immunopositive to ET-1 (36% ± 4, n = 4 versus 56% ± 3, n = 4, P < 0.01). The distribution of vasopressin-immunopositive cells was unchanged by capsaicin treatment (10% ±...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"63 1","pages":"71-76"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89124944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：