A. Osińska, P. Jachimowicz, Sebastian Niestępski, M. Harnisz, E. Korzeniewska
This study aimed to perform a qualitative and a quantitative assessment of the prevalence of genes encoding resistance to beta-lactam, tetracycline, and chloramphenicol antibiotics in samples of DNA isolated from air in a municipal wastewater treatment plant (WWTP) and a municipal waste management plant (WMP). Air samples were collected in the mechanical (MP) and biological (BP) processing units of WWTP and WMP in winter and spring. The samples of air were collected by impingement into PBS solution and subsequently, DNA was isolated. The prevalence of the 16S r RNA gene and ARGs was determined by PCR, and the most abundant ARGs were quantified by qPCR. The highest diversity of the analyzed ARGs was noted in air samples collected in the mechanical processing units of the WWTP (winter) and the WMP (spring). The copy of ARGs varied between treatment units and seasons. ARGs were most abundant in air samples collected in spring in the MP units of both the WWTP and the WMP. The study demonstrated that ARGs are ubiquitous in the air in both WWTPs and WMPs. The presence of ARGs in the air can exert a negative impact on the health of plant employees.
本研究旨在定性和定量评估从城市污水处理厂(WWTP)和城市废物处理厂(WMP)的空气中分离的DNA样本中编码β -内酰胺、四环素和氯霉素抗生素耐药基因的流行程度。冬季和春季分别在污水处理厂和污水处理厂的机械(MP)和生物(BP)处理单元采集空气样本。通过撞击PBS溶液收集空气样本，随后分离DNA。PCR检测16S r RNA基因和ARGs的流行率，qPCR检测最丰富的ARGs。在污水处理厂(冬季)和水处理厂(春季)的机械处理单元收集的空气样本中，所分析的ARGs的多样性最高。arg的副本在治疗单位和季节之间有所不同。春季收集的空气样本中ARGs含量最高的是污水处理厂和水处理厂的MP单元。该研究表明，在污水处理厂和水处理厂的空气中，arg无处不在。空气中ARGs的存在会对工厂员工的健康产生负面影响。
{"title":"The effects of season and processing technology on the abundance of antibiotic resistance genes in air samples from municipal wastewater treatment and waste management plants","authors":"A. Osińska, P. Jachimowicz, Sebastian Niestępski, M. Harnisz, E. Korzeniewska","doi":"10.37190/EPE210108","DOIUrl":"https://doi.org/10.37190/EPE210108","url":null,"abstract":"This study aimed to perform a qualitative and a quantitative assessment of the prevalence of genes encoding resistance to beta-lactam, tetracycline, and chloramphenicol antibiotics in samples of DNA isolated from air in a municipal wastewater treatment plant (WWTP) and a municipal waste management plant (WMP). Air samples were collected in the mechanical (MP) and biological (BP) processing units of WWTP and WMP in winter and spring. The samples of air were collected by impingement into PBS solution and subsequently, DNA was isolated. The prevalence of the 16S r RNA gene and ARGs was determined by PCR, and the most abundant ARGs were quantified by qPCR. The highest diversity of the analyzed ARGs was noted in air samples collected in the mechanical processing units of the WWTP (winter) and the WMP (spring). The copy of ARGs varied between treatment units and seasons. ARGs were most abundant in air samples collected in spring in the MP units of both the WWTP and the WMP. The study demonstrated that ARGs are ubiquitous in the air in both WWTPs and WMPs. The presence of ARGs in the air can exert a negative impact on the health of plant employees.","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of the undertaken investigations was to examine in laboratory conditions whether an introduction of an assisting opoka rock layer with the granulation of 1–6 mm into the ground improves the removal efficiency of nitrogen and phosphorus compounds from domestic sewage. The performed investigations concerned the layer supporting the removal efficiency of domestic sewage in a home sewage treatment plant under infiltration drainage. The model investigations of wastewater purification were carried out in a medium sand bed with an assisting, 0.10 and 0.20 m thick opoka rock layer. The effectiveness of wastewater purification related to basic qualitative indicators (total suspended solids – TSS, BOD 5 , COD, total nitrogen, total phosphorus) was in line with the Polish standards on sewage disposal into grounds and surface water. The medium sand soil bed with the 0.20 m thick assisting opoka rock layer showed higher effectiveness of wastewater purification than that 0.10 m thick. The application of the 0.20 m thick opoka rock layer increased the removal efficiency regarding TSS by 6.2%, total nitrogen by 20.4%, ammonium nitrogen by 8.3% and total phosphorus by 2.9%, and removal efficiency regarding BOD 5 by 1.2% and COD by 1.9% with relation to the 0.10 m thick assisting layer (all percentages − in average). The results confirm that the natural opoka rock with the granulation of 1–6 mm can be used to assist in the removal of nitrogen and phosphorus compounds from wastewater with the application of infiltration drainage.
{"title":"Investigations on the effectiveness of wastewater purification in medium sand with assisting opoka rock layer","authors":"Marek Kalenik, M. Chalecki","doi":"10.37190/EPE210105","DOIUrl":"https://doi.org/10.37190/EPE210105","url":null,"abstract":"The objective of the undertaken investigations was to examine in laboratory conditions whether an introduction of an assisting opoka rock layer with the granulation of 1–6 mm into the ground improves the removal efficiency of nitrogen and phosphorus compounds from domestic sewage. The performed investigations concerned the layer supporting the removal efficiency of domestic sewage in a home sewage treatment plant under infiltration drainage. The model investigations of wastewater purification were carried out in a medium sand bed with an assisting, 0.10 and 0.20 m thick opoka rock layer. The effectiveness of wastewater purification related to basic qualitative indicators (total suspended solids – TSS, BOD 5 , COD, total nitrogen, total phosphorus) was in line with the Polish standards on sewage disposal into grounds and surface water. The medium sand soil bed with the 0.20 m thick assisting opoka rock layer showed higher effectiveness of wastewater purification than that 0.10 m thick. The application of the 0.20 m thick opoka rock layer increased the removal efficiency regarding TSS by 6.2%, total nitrogen by 20.4%, ammonium nitrogen by 8.3% and total phosphorus by 2.9%, and removal efficiency regarding BOD 5 by 1.2% and COD by 1.9% with relation to the 0.10 m thick assisting layer (all percentages − in average). The results confirm that the natural opoka rock with the granulation of 1–6 mm can be used to assist in the removal of nitrogen and phosphorus compounds from wastewater with the application of infiltration drainage.","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70006199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to evaluate the vermicompost quality of sewage sludge (SS) mixed with sugar beet pulp (SBP) and cow dung (CD) wastes employing Eisenia fetida. The basic issue faced in the wastewater treatment plant is how to reduce the amount of sewage sludge? This paper discusses an insitu vermicomposting approach for the reuse of sewage sludge. A total of 11 reactors containing various waste combinations were organized and changes in physicochemical parameters were evaluated during 90 days of the experiment. The results indicated that SS could be converted into a good quality product by vermicomposting if suitable mixing quantities are provided (50% SS, 25% SBP, 25% CD). Vermicomposting caused significant reductions in pH, TOC, the content of heavy metals, and C/N ratio, while electric conductivity and NPK content increased as compared to initial mixtures. The pre-sent study implied that the application of sewage sludge in the agricultural fields after vermicomposting could be a suitable and usable approach for sewage disposal.
{"title":"Change of physichochemical properties and heavy metals content in sewage sludge during its vermicomposting with Eisenia fetida","authors":"Sukru Taner Azgin","doi":"10.37190/EPE210102","DOIUrl":"https://doi.org/10.37190/EPE210102","url":null,"abstract":"This study aimed to evaluate the vermicompost quality of sewage sludge (SS) mixed with sugar beet pulp (SBP) and cow dung (CD) wastes employing Eisenia fetida. The basic issue faced in the wastewater treatment plant is how to reduce the amount of sewage sludge? This paper discusses an insitu vermicomposting approach for the reuse of sewage sludge. A total of 11 reactors containing various waste combinations were organized and changes in physicochemical parameters were evaluated during 90 days of the experiment. The results indicated that SS could be converted into a good quality product by vermicomposting if suitable mixing quantities are provided (50% SS, 25% SBP, 25% CD). Vermicomposting caused significant reductions in pH, TOC, the content of heavy metals, and C/N ratio, while electric conductivity and NPK content increased as compared to initial mixtures. The pre-sent study implied that the application of sewage sludge in the agricultural fields after vermicomposting could be a suitable and usable approach for sewage disposal.","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70006153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikolinka Doneva, A. Zendelska, M. Hadži-Nikolova, D. Mirakovski, G. Dimov
Municipal solid waste management is still one of the major environmental challenges at a national level, and although with national and regional waste plans in place, the Republic of North Macedonia with its eight regions has only one standard landfill active, and at least 54 non-standard municipal landfills and hundreds of dump sites and old landfills. In the light of the soon expected regional establishment of an integrated and self-sustainable waste management system in the eastern and north-eastern regions, to increase awareness by the local population and hospitality enterprises for source separation schemes of organic waste, as largest producers of organic waste, Goce Delcev & Hellenic Mediterranean Universities together with Municipality of Katerini, Municipality of Yermasoyia and Municipality of Probistip launched joint project co-funded by EU, Utilizing pay-as-you-throw Systems and Autonomous Composting Units for Biowastes Management in Touristic Areas (BIOWASTE). This paper aims to present the environmental benefits of the project implementation and scaling up process that was developed, aiming to expand the BIOWASTE project processes in the whole Municipality of Probishtip.
{"title":"Implementation of innovative technologies pay-as-you-throw and autonomous composting units for biowaste management. scaling up the biowaste project in the municipality of probishtip, Republic of North Macedonia","authors":"Nikolinka Doneva, A. Zendelska, M. Hadži-Nikolova, D. Mirakovski, G. Dimov","doi":"10.37190/epe210305","DOIUrl":"https://doi.org/10.37190/epe210305","url":null,"abstract":"Municipal solid waste management is still one of the major environmental challenges at a national level, and although with national and regional waste plans in place, the Republic of North Macedonia with its eight regions has only one standard landfill active, and at least 54 non-standard municipal landfills and hundreds of dump sites and old landfills. In the light of the soon expected regional establishment of an integrated and self-sustainable waste management system in the eastern and north-eastern regions, to increase awareness by the local population and hospitality enterprises for source separation schemes of organic waste, as largest producers of organic waste, Goce Delcev & Hellenic Mediterranean Universities together with Municipality of Katerini, Municipality of Yermasoyia and Municipality of Probistip launched joint project co-funded by EU, Utilizing pay-as-you-throw Systems and Autonomous Composting Units for Biowastes Management in Touristic Areas (BIOWASTE). This paper aims to present the environmental benefits of the project implementation and scaling up process that was developed, aiming to expand the BIOWASTE project processes in the whole Municipality of Probishtip.","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Valorisation of sewage sludge humic compounds in the aspect of its application in natural environment","authors":"M. Jakubus, E. Bakinowska, E. Tobiášová","doi":"10.37190/EPE210106","DOIUrl":"https://doi.org/10.37190/EPE210106","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70006204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Rouhani, S. Azizi, M. Maaza, B. Mamba, T. Msagati
{"title":"Covalent immobilization of laccase on Fe3O4–graphene oxide nanocomposite for biodegradation of phenolic compounds","authors":"S. Rouhani, S. Azizi, M. Maaza, B. Mamba, T. Msagati","doi":"10.37190/EPE210107","DOIUrl":"https://doi.org/10.37190/EPE210107","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"47 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70006216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The benefits of partial substitution of fossil fuel with alternative fuel in cement plant. Case study","authors":"M. Żygadło, P. Purgał","doi":"10.37190/epe200101","DOIUrl":"https://doi.org/10.37190/epe200101","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"46 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Separation and concentration of cationic surfactant solutions with the use of ceramic modules","authors":"A. Klimonda, I. Kowalska","doi":"10.37190/epe200203","DOIUrl":"https://doi.org/10.37190/epe200203","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"36 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seasonal variabilitiy of antibiotic resistance and biodiversity of tap water bacteria in Wrocław, Poland","authors":"A. Siedlecka, M. Wolf-Baca, Katarzyna Pierkarska","doi":"10.37190/epe200207","DOIUrl":"https://doi.org/10.37190/epe200207","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"46 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Energy and environmental analysis of a CCHP system used in industrial facility","authors":"A. Bugaj","doi":"10.37190/epe200307","DOIUrl":"https://doi.org/10.37190/epe200307","url":null,"abstract":"","PeriodicalId":11709,"journal":{"name":"Environment Protection Engineering","volume":"59 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70005965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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