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Comparison of the Sanitary Conditions of Raw Milk Cheese and Pasteurized Milk Cheese Sold in the Market: A Preliminary Study 市场上销售的生奶奶酪与巴氏杀菌奶酪卫生状况的比较:初步研究
Pub Date : 2019-03-01 DOI: 10.22424/JMSB.2019.37.1.33
Hyun-Woo Lim, Se-Hyung Kim, Jung-Whan Chon, Dongryeoul Bae, K. Song, Dongkwan Jeong, K. Seo
The purpose of this study was to explore the microbiological safety of various cheeses made from raw milk currently imported and sold in Korea. A total of 12 cheeses were considered for the study 4 kinds of imported cheese made from raw milk, 4 kinds of imported cheese made from pasteurized milk, and 4 kinds of domestic cheese made from pasteurized milk, and aerobic count, presence of E. coli, Salmonella spp., and Listeria monocytogenes were investigated. Results from this study showed that in the 12 different kinds of cheese tested, E. coli, Salmonella spp., and Listeria monocytogenes were negative. Therefore, the sanitary conditions of imported cheese made from raw milk, imported cheese made from pasteurized milk, and domestic cheese made from pasteurized milk are considered safe. The mean aerobic count in the 12 types of tested cheese was below 4.0 log CFU/g. In conclusion, various cheeses produced from raw milk or pasteurized milk currently sold in Korea have been found to be hygienic and safe. In the future, raw milk cheeses produced and sold in Korea will require long-term research not only to ensure safety but also to produce new, high-quality products.
本研究的目的是探讨目前在韩国进口和销售的各种原料牛奶制成的奶酪的微生物安全性。选取4种进口生乳奶酪、4种进口巴氏奶奶酪和4种国产巴氏奶奶酪共12种奶酪,对好氧计数、大肠杆菌、沙门氏菌和单核增生李斯特菌的存在情况进行了调查。本研究结果表明,在12种不同的奶酪中,大肠杆菌、沙门氏菌和单核细胞增生李斯特菌均呈阴性。因此,进口生奶奶酪、进口巴氏奶奶酪和国产巴氏奶奶酪的卫生条件都是安全的。12种奶酪的平均有氧计数低于4.0 log CFU/g。由此可见,目前在国内销售的各种原料奶和巴氏杀菌奶制成的奶酪是卫生安全的。今后,在国内生产和销售的原料奶奶酪不仅要确保安全性,还要进行长期研究,以生产出新的高质量产品。
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引用次数: 3
Occurrence and Countermeasures for Aflatoxin M1 in Milk and Milk Products: A Review 牛奶及乳制品中黄曲霉毒素M1的发生及防治措施综述
Pub Date : 2019-03-01 DOI: 10.22424/JMSB.2019.37.1.1
Jung-Whan Chon, Dongryeoul Bae, K. Song, Dongkwan Jeong, K. Seo
In general, milk is a nutritious food and is very helpful in improving and maintaining a person's health. However, over the past 30 years, a number of studies have reported the presence of Aflatoxin M1 in milk and milk products worldwide. The contamination with Aflatoxin M1 in milk and dairy products has become an important issue all over the world. The presence of the mycotoxin in these products was a major concern particularly among children and infants, who are more sensitive than adults. This study reviewed recent data to summarize the current status of Aflatoxin M1 in milk and dairy products produced in various regions around the world, related regulations, reduction strategies, detection methods, and future research tasks. Strict regulation and superior milk-handling techniques are essential to minimize Aflatoxin M1 contamination in milk and dairy products using ever-evolving analytical techniques. Furthermore, education should be imparted to ensure that Aflatoxin M1 that may exist in milk and dairy products and its effects on human health are made aware of.
总的来说,牛奶是一种营养丰富的食物,对改善和维持一个人的健康非常有帮助。然而,在过去的30年里,许多研究报道了世界各地的牛奶和奶制品中存在黄曲霉毒素M1。牛奶和奶制品中的黄曲霉毒素M1污染已成为世界范围内的一个重要问题。这些产品中霉菌毒素的存在是一个主要问题,特别是儿童和婴儿,他们比成年人更敏感。本研究回顾了近年来的相关资料,总结了世界各地区牛奶及乳制品中黄曲霉毒素M1的现状、相关法规、减少策略、检测方法以及未来的研究任务。严格的法规和卓越的牛奶处理技术是必不可少的,以尽量减少黄曲霉毒素M1污染的牛奶和乳制品使用不断发展的分析技术。此外,应开展教育,确保人们了解牛奶和奶制品中可能存在的黄曲霉毒素M1及其对人类健康的影响。
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引用次数: 0
Serum Cholesterol-lowering Effect of Fermented Milk and Effect of Intestinal Microflora Composition on Function of Fermented Milk 发酵乳的血清降胆固醇作用及肠道菌群组成对发酵乳功能的影响
Pub Date : 2019-03-01 DOI: 10.22424/JMSB.2019.37.1.27
Yu-Jin Kim, Y. Yoon, Soomin Lee
Fermented milk has been developed with its functionalities, and its health-promoting ability has been spotlighted due to its relationship with diseases such as cancer, cardiovascular disease, and diabetes, and gut microbiota. As national burden of cardiovascular disease increases over time, there is a need to prevent hypercholesterolemia. To achieve that, gut microbiota, which is altered by host’s diet and environment, plays important roles in lowering cholesterol in the blood. Moreover, fermented milk may be effective as a cholesterol-lowering agent by altering gut microbiota composition. Gut microbiota may alter not only functions of the fermented milk but also bio-accessibility of functional materials. These results suggested that gut microbiota composition influences the impact of fermented milk. Thus, we should understand how functional materials are degraded by gut microbiota and absorbed into the gut.
发酵乳因其功能而得到发展,其促进健康的能力因其与癌症、心血管疾病、糖尿病和肠道微生物群等疾病的关系而受到关注。随着国家心血管疾病负担的增加,有必要预防高胆固醇血症。为了实现这一目标,肠道微生物群在降低血液中的胆固醇方面发挥着重要作用,而肠道微生物群会被宿主的饮食和环境所改变。此外,发酵乳可能通过改变肠道菌群组成而有效地作为降胆固醇剂。肠道菌群不仅可以改变发酵乳的功能,还可以改变功能材料的生物可及性。这些结果表明,肠道菌群组成影响发酵乳的效果。因此,我们应该了解功能材料是如何被肠道微生物群降解并吸收到肠道中的。
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引用次数: 1
The spy-gfp Operon Fusion in Salmonella Enteritidis and Salmonella Gallinarum Senses the Envelope Stress 肠炎沙门氏菌和鸡沙门氏菌的spy-gfp操纵子融合检测包膜应激
Pub Date : 2018-12-01 DOI: 10.22424/JMSB.2018.36.4.208
Bo Gyeong Kang, I. Bang
Emergence of drug resistant strains of Salmonella enterica threatens milk processing and related dairy industries, thereby increasing the need for development of new anti-bacterials. Developments of antibacterial drugs are largely aimed to target the bacterial envelope, but screening their efficacy on bacterial envelope is laborious. This study presents a potential biosensor for envelope-specific stress in which a gfp reporter gene fused to spy gene encoding a periplasmic chaperone protein Spy (spheroplast protein y) that can sense envelope stress signals transduced by two major two-component signal transduction systems BaeSR and CpxAR in Salmonella enterica serovars Enteritidis and S. Gallinarum. Using spy-gfp operon fusions in S. Enterititis and S. Gallinarum, we found that spy transcription in both serovars was greatly induced when Salmonella cells were forming the spheroplast and were treated with ethanol or a membrane-disrupting antibiotic polymyxin B. These envelope stress-specific inductions of spy transcription were abrogated in mutant Salmonella lacking either BaeR or CpxR. Results illustrate that induction of Spy expression can be efficiently triggered by two-component signal transduction systems sensing envelope stress conditions, and thereby suggest that monitoring the spy transcription by spy-gfp operon fusions would be helpful to determine if developing antimicrobials can damage envelopes of S. Enteritidis and S. Gallinarum.
肠道沙门氏菌耐药菌株的出现威胁着牛奶加工和相关乳制品行业,从而增加了开发新型抗菌药物的需求。抗菌药物的发展在很大程度上是针对细菌包膜的,但筛选它们对细菌包膜的疗效是非常困难的。本研究提出了一种潜在的包膜特异性应激生物传感器,其中gfp报告基因融合到编码外质伴侣蛋白spy(球体蛋白y)的基因中,该基因可以感知肠道沙门氏菌血清型肠炎沙门氏菌和鸡沙门氏菌中两个主要的双组分信号转导系统BaeSR和CpxAR转导的包膜应激信号。在肠炎沙门氏菌和鸡链球菌中使用spy-gfp操纵子融合,我们发现,当沙门氏菌细胞形成球质体并被乙醇或破坏膜的抗生素多粘菌素b处理时,这两种血清型的间谍转录都被极大地诱导,这些包膜胁迫特异性的间谍转录诱导在缺乏BaeR或CpxR的突变沙门氏菌中被取消。结果表明,通过双组分信号转导系统感应包膜胁迫条件可以有效地诱导Spy表达,从而表明通过Spy -gfp操纵子融合监测Spy转录将有助于确定开发的抗菌剂是否会破坏肠炎沙门氏菌和鸡链球菌的包膜。
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引用次数: 0
Rheological Properties and Fatty Acid Profile of Farm Butter Made from Cows’ Milk Grazing on Mountain Pasture 山地牧场放牧牛乳制黄油流变学特性及脂肪酸谱
Pub Date : 2018-12-01 DOI: 10.22424/JMSB.2018.36.4.196
S. Park, Bae-Hun Lee, Hyo-Jin Gang, Gur-Yoo Kim
This study was carried out to investigate the rheological properties and composition of unsaturated fatty acid of farm butter made from the milk of cows grazing at high mountain pasture in Pyronogchang SKY ranch (above sea level, 935 m). From two groups containing 7 cows each, a group was fed in-door with TMR (total mixed ration) feed and whereas the other group was grazed pastures for 12 h. The daily intake of feed on basis of dry matter (DMI), milk yields, concentration of milk constituents, and fatty acid profile of pasture milk were compared with control TMR milk. In addition, the physiochemical properties and composition of unsaturated fatty acids of the butter were also compared with those of the butter made from control TMR milk. Upon comparison, the health-promoting index (HPI) of fatty acids; the ratio of omega-6 fatty acids to omega-3 fatty acids (n-6 to n-3 UFA), the atherogenicity index (AI), and the ratio of linoleic acid to α-linolenic acid (LA to ALA) was apparently improved in farm butter than those of control butter. Thus, it could make the dairy farm visitors to consume the farm butter containing the health-promoting fatty acids from the milk of cows grazing on mountain pasture.
本研究进行了探讨流变特性和不饱和脂肪酸的组成的农场黄油的牛奶在天空Pyronogchang高山牧场的奶牛放牧的牧场(海拔935米)。从包含7牛两组,一组是美联储室内咯(总混合配给)提要,而另一组是放牧牧场12 h。饲料干物质的基础上的日常摄入量(DMI),牛奶产量,牛奶成分的浓度,与对照TMR奶进行脂肪酸谱比较。此外,还与对照TMR牛奶制作的黄油进行了理化性质和不饱和脂肪酸组成的比较。脂肪酸的健康促进指数(HPI);农场黄油的ω -6脂肪酸与ω -3脂肪酸之比(n-6与n-3 UFA)、动脉粥样硬化指数(AI)和亚油酸与α-亚麻酸之比(LA与ALA)均明显高于对照黄油。因此,它可以使奶牛场的游客消费含有从山上牧场放牧的奶牛的牛奶中促进健康的脂肪酸的农场黄油。
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引用次数: 2
Shelf-Life Extension and Increase in Survivability of Probiotics Powder by Ultrasonic Treatment 超声波处理延长益生菌粉的保质期和提高其存活能力
Pub Date : 2018-12-01 DOI: 10.22424/jmsb.2018.36.4.220
D. Hong, Seong-Eun Jung, M. Lee, Ho-Jin Lee, Jae-Ho Lee, Guknam Na, Il-dong Choi, Jung-Lyoul Lee, J. Sim
The purpose of this study was to investigate the effect of ultrasonic treatment during probiotics production process on the shelf life and the survival rate. Once Lactobacillus plantarum and Bifidobacterium longum were cultured in edible culture medium, ultrasonic treatment was performed at 100 Hz for 1, 2, 5, 7, and 10 minutes. Ultrasonic-treated L. plantarum HY7715 and B. longum HY8001 were centrifuged, mixed with a cryoprotectant, and lyophilized. The prepared lactic acid bacteria powder was individually packaged. After 6 months, viable cell counts were measured separately under cold storage and room temperature. In vitro digestion experiments were performed to determine the survival rate at digestive tract. As a result, it was observed that shelf life and survival rate were increased compared to untreated control group.
本研究旨在探讨益生菌生产过程中超声波处理对其保质期和成活率的影响。一旦植物乳杆菌和长双歧杆菌在可食用培养基中培养,在100 Hz下进行超声波处理,分别为1、2、5、7和10分钟。超声处理的植物乳杆菌HY7715和长芽孢杆菌HY8001离心,混合冷冻保护剂,冻干。将制备的乳酸菌粉进行单独包装。6个月后,分别在冷藏和室温条件下测定活细胞计数。体外消化实验测定其在消化道的存活率。结果,观察到与未经处理的对照组相比,货架期和存活率有所增加。
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引用次数: 1
Development of Food-Grade Nano-Delivery Systems and Their Application to Dairy Foods: A Review 食品级纳米输送系统的发展及其在乳制品中的应用综述
Pub Date : 2018-12-01 DOI: 10.22424/jmsb.2018.36.4.187
Ho-Kyung Ha, Won-Jae Lee
Nano-delivery systems, such as nanoparticles, nanoemulsions, and nanoliposomes, are carriers that have been used to enhance the chemical as well as physical stability and bioavailability of bioactive compound. Food-grade nano-delivery system can be produced with edible biopolymers including proteins and carbohydrates. In addition to the lowtoxicity, biocompatibility, and biodegradability of these biopolymers, their functional characteristics, such as their ability to bind hydrophobic bioactive compounds and form a gel, make them potential and ideal candidates for the fortification of bioactive compounds in functional dairy foods. This review focuses on different types of nano-delivery systems and edible biopolymers as delivery materials. In addition, the applications of food-grade nano-delivery systems to dairy foods are also described.
纳米递送系统,如纳米颗粒、纳米乳剂和纳米脂质体,是用于提高生物活性化合物的化学和物理稳定性和生物利用度的载体。食品级纳米输送系统可以用可食用的生物聚合物生产,包括蛋白质和碳水化合物。除了这些生物聚合物的低毒性、生物相容性和生物可降解性外,它们的功能特性,例如它们结合疏水生物活性化合物并形成凝胶的能力,使它们成为功能性乳制品中强化生物活性化合物的潜在和理想候选者。本文综述了不同类型的纳米递送系统和作为递送材料的可食用生物聚合物。此外,还介绍了食品级纳米输送系统在乳制品中的应用。
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引用次数: 1
Breastfeeding and Melatonin 母乳喂养和褪黑素
Pub Date : 2018-09-01 DOI: 10.22424/jmsb.2018.36.3.133
Minyu Song, Wonseo Park, Jayeon Yoo, J. Ham
Breastfeeding is highly recommended due to its benefits for both the infant and mother; however, most mothers predominantly use formula feed. Breastfeeding affords protection against a wide variety of medical conditions that may emerge at different time points over the lifespan, including hospital admissions for respiratory infections and neonatal fever, offspring childhood obesity, and cancer as well as cardiovascular disease, hyperlipidemia, hypertension, and diabetes. Moreover, breastfeeding is expected to decrease the risk of adolescent depression and other psychopathologies. It is also important for the development of the gut, gut-brain axis, and immune system, and night-time breast milk is likely to have higher antioxidant, anti-inflammatory, and immune regulatory effects due to the impact of breast milk melatonin on the infant's developing microbiome and gut permeability. Melatonin can be added to a night-time-specific formula feed; however, it is not included in the Korean Food Additive Codex.
强烈建议母乳喂养,因为它对婴儿和母亲都有好处;然而,大多数母亲主要使用配方饲料。母乳喂养可以预防生命中不同时间点可能出现的各种疾病,包括因呼吸道感染和新生儿发烧、后代儿童肥胖、癌症以及心血管疾病、高脂血症、高血压和糖尿病而入院。此外,母乳喂养有望降低青少年抑郁症和其他精神疾病的风险。它对肠道、肠脑轴和免疫系统的发育也很重要,由于母乳褪黑素对婴儿微生物群发育和肠道通透性的影响,夜间母乳可能具有更高的抗氧化、抗炎和免疫调节作用。褪黑素可以添加到夜间专用配方饲料中;但在韩国食品添加剂标准中却没有。
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引用次数: 0
Erratum to: Objective Measurements of Textural and Rheological Properties of Cheese 奶酪结构和流变特性客观测量的勘误
Pub Date : 2018-09-01 DOI: 10.22424/jmsb.2018.36.3.186
Mee-Ryung Lee
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引用次数: 1
Antibacterial Activity of Crude Aronia melanocarpa (Black Chokeberry) Extracts against Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis in Various Dairy Foods: Preliminary Study 黑樱桃粗提取物对各种乳制品中蜡样芽孢杆菌、金黄色葡萄球菌、阪崎克罗诺杆菌和肠炎沙门氏菌的抑菌活性:初步研究
Pub Date : 2018-09-01 DOI: 10.22424/JMSB.2018.36.3.155
Dong‐Hyeon Kim, Hyun-Woo Lim, Se-Hyung Kim, Jun-Man Lee, Jung-Whan Chon, K. Song, Dongryeoul Bae, Jinhyun Kim, Hyunsook Kim, K. Seo
In this study, the antibacterial activity of Aronia melanocarpa (black chokeberry) ethanol extract against Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis was investigated using the spot-on-lawn assay. The results showed that this extract exhibited antibacterial activities against Bacillus cereus (complete inhibition) and Staphylococcus aureus (partial inhibition), but did not inhibit the growth of Cronobacter sakazakii and Salmonella Enteritidis. This study shows that the Aronia melanocarpa (black chokeberry) ethanol extract was more effective against Gram-positive bacteria than Gramnegative bacteria. Hence, it is suggested that Aronia melanocarpa could be a useful food supplement, and could be utilized as a naturally derived additive for maintaining the safety of various dairy products. Furthermore, future research should be conducted to examine the possibility of using such products as functional ingredients for improving the antioxidative and anti-inflammatory activities of food products.
本研究采用现场草坪法研究了黑莓乙醇提取物对蜡样芽孢杆菌、金黄色葡萄球菌、阪崎克罗诺杆菌和肠炎沙门氏菌的抑菌活性。结果表明,该提取物对蜡样芽孢杆菌(完全抑制)和金黄色葡萄球菌(部分抑制)具有抑菌活性,但对阪崎克罗诺杆菌和肠炎沙门氏菌的生长没有抑制作用。本研究表明,黑莓乙醇提取物对革兰氏阳性菌的抑菌效果优于革兰氏阴性菌。综上所述,黑油桃可能是一种有益的食品补充剂,可以作为一种天然衍生添加剂来维持各种乳制品的安全。此外,未来的研究应探讨使用这些产品作为功能性成分的可能性,以提高食品的抗氧化和抗炎活性。
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引用次数: 7
期刊
Journal of Milk Science and Biotechnology
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