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Formation and Characterization of Casein Phosphopeptide/Chitosan Oligosaccharide NanoComplex 酪蛋白磷酸肽/壳聚糖纳米复合物的形成与表征
Pub Date : 2018-09-01 DOI: 10.22424/JMSB.2018.36.3.164
Yunjung Baek, Ho-Kyung Ha, Ji-Hong Lee, Mee-Ryung Lee, Won-Jae Lee
The objectives of this study were to manufacture casein phosphopeptide (CPP)/chitosan oligosaccharide (CSO) nanocomplexes and to investigate the impacts of manufacturing variables, such as CPP concentration and pH, on their morphological and physicochemical characteristics. Transmission electron microscopy (TEM) and particle size analysis were used to assess the morphological and physicochemical properties of the CPP/CSO nanocomplexes, respectively. Based on the images obtained by TEM, the spherical shapes of the CPP/CSO nanocomplexes ranged from 50 to 150 nm. As the concentration of CPP was increased and the pH was decreased, the average particle size of the nanocomplexes significantly (p<0.05) increased. The CPP/CSO nanocomplexes had a highly uniform distribution with a polydispersity index value of less than 0.3. In addition, they had a negative surface charge with a zeta-potential value between —17 and —26 mV. The CPP/CSO nanocomplexes showed good stability during the freeze-drying process. In conclusion, CPP/CSO nanocomplexes were successfully manufactured, and the CPP concentration and pH were found to be key factors that affected their morphological and physicochemical properties.
本研究的目的是制备酪蛋白磷酸肽(CPP)/壳寡糖(CSO)纳米复合物,并研究CPP浓度和pH等制备变量对其形态和物理化学特性的影响。利用透射电子显微镜(TEM)和粒径分析分别对CPP/CSO纳米配合物的形态和理化性质进行了表征。根据TEM图像,CPP/CSO纳米配合物的球形范围在50 ~ 150 nm之间。随着CPP浓度的增加和pH的降低,纳米复合物的平均粒径显著增加(p<0.05)。CPP/CSO纳米配合物分布高度均匀,多分散性指数小于0.3。此外,它们具有负表面电荷,ζ电位值在-17和-26 mV之间。CPP/CSO纳米配合物在冷冻干燥过程中表现出良好的稳定性。综上所述,CPP/CSO纳米配合物制备成功,CPP浓度和pH是影响其形态和理化性质的关键因素。
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引用次数: 1
Development of a Functional Mixed-Starter Culture for Kefir Fermentation 开菲尔发酵功能混合发酵剂的研制
Pub Date : 2018-09-01 DOI: 10.22424/JMSB.2018.36.3.178
Bomee Lee, H. Yi, Yong-II Moon, Sejong Oh
Kefir, which originates in the Caucasian mountains, is a cultured milk beverage produced by a combination of acidic and alcoholic fermentation. Kefir products are commonly used as food vehicles to deliver health-promoting materials including kefran and lactic acid bacteria to consumers. The aim of this study was to develop a freeze-dried starter culture without yeast and assess the suitability of kefir-like dairy products for the growth of lactic acid bacteria and the acidification of milk. Pasteurized whole milk (SNF 8.5%) stored at 25°C was aseptically inoculated with starter cultures (0.002% w/v); it was kept at 25°C until the pH attained a value of 4.6. Ten grams of the kefir-like product sample was diluted with 90 mL of 0.15% peptone water diluent in a milk dilution bottle, followed by uniform mixing for 1 min. Viable cells of Lactobacillus species were enumerated on modified-MRS agar (pH 5.2), with incubation at 37°C for 48 h. Viable cells of Lactococcus species were enumerated on M17-lactose agar, with incubation at 32°C for 48 h. The pH attained a value of 4.6 after fermentation for 9 h 30 min (Starter 1), 9 h 45 min (Starter 2), and 12 h (Starter 3). The viable cell count of Lactobacillus sp. and Lactococcus sp. was initially 10∼10 CFU/g; it increased significantly to 10 CFU/g after 12 h of incubation. During the storage of the kefir-like products at 4°C for 1 4 days, the total viable cell numbers were unchanged, but the pH decreased slightly. The consistency of the kefir products increased gradually during the storage. The organoleptic properties of the kefir products fermented using the new starter culture are more desirable than those of commercial kefir. These results suggest that the newly developed starter culture without yeast could be suitable for kefir fermentation.
开菲尔起源于高加索山脉,是一种由酸性和酒精发酵结合而成的人工培养乳饮料。开菲尔产品通常被用作食品载体,向消费者提供包括克弗兰和乳酸菌在内的促进健康的物质。本研究的目的是开发一种不含酵母的冻干发酵剂,并评估类似开菲尔的乳制品对乳酸菌生长和牛奶酸化的适宜性。25℃下保存的巴氏全脂牛奶(SNF 8.5%)用发酵剂(0.002% w/v)无菌接种;保持在25℃,直到pH值达到4.6。十克kefir-like产品样本和90毫升的0.15%蛋白胨水稀释剂稀释牛奶稀释瓶,其次是均匀混合1分钟。可行的乳酸菌的细胞被枚举modified-MRS琼脂(pH值5.2),与孵化37°C 48 h。可行的细胞Lactococcus物种枚举M17-lactose琼脂,潜伏在32°C 48 h。pH值达到一个值的4.6发酵后9 h 30分钟(起动器1),9小时45分钟(起动器2),和12 h (Starter 3)。乳杆菌和乳球菌的活细胞计数最初为10 ~ 10 CFU/g;孵育12 h后显著增加至10 CFU/g。在4℃条件下保存14天,活细胞总数没有变化,但pH值略有下降。在贮存过程中,克非尔产品的稠度逐渐增加。使用新发酵剂发酵的克非尔产品的感官特性比商品克非尔更理想。上述结果表明,新开发的无酵母发酵剂适合开菲尔发酵。
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引用次数: 4
Antimicrobial Effect of Mentha piperita (Peppermint) Oil against Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis in Various Dairy Foods: Preliminary Study 薄荷油对各种乳制品中蜡样芽孢杆菌、金黄色葡萄球菌、阪崎克罗诺杆菌和肠炎沙门氏菌的抑菌作用:初步研究
Pub Date : 2018-09-01 DOI: 10.22424/JMSB.2018.36.3.146
Hyun-Woo Lim, Dong‐Hyeon Kim, Se-Hyung Kim, Jun-Man Lee, Jung-Whan Chon, K. Song, Dongryeoul Bae, Jinhyun Kim, Hyunsook Kim, K. Seo
There are more than 25 species of Mentha plants, which are aromatic perennial herbs. Currently, these species are being widely used with great interest because of various clinical findings regarding their health benefits. This is due to the abundance of volatile compounds that could expedite environmental interactions such as protection against herbivores, parasites, pathogens, and so on. Therefore, in this study, the antimicrobial effect of Mentha piperita (peppermint) oil on Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis were investigated using the spot-on-lawn method. The results show that Mentha piperita (peppermint) oil exhibited antimicrobial activities against Bacillus cereus, Staphylococcus aureus, and Cronobacter sakazakii; however, it did not inhibit the growth of Salmonella Enteritidis. This shows that the antimicrobial effect of Mentha piperita (peppermint) oil is effective against both Gram-positive and Gramnegative bacteria. Hence, in the present study, Mentha piperita (peppermint) oil was shown to have strong antimicrobial activities; it could be used as a potential food additive for improving the quality of various milk-based products due to its various bioactive properties. Future studies should be conducted for manufacturing functional dairy products with the addition of peppermint oil to prevent and/or alleviate specific diseases.
薄荷属植物有25种以上,是芳香的多年生草本植物。目前,由于各种关于其健康益处的临床发现,这些物种正被广泛使用。这是由于丰富的挥发性化合物可以加速环境的相互作用,如对食草动物、寄生虫、病原体等的保护。因此,本研究采用草坪现场法,考察薄荷油对蜡样芽孢杆菌、金黄色葡萄球菌、阪崎克罗诺杆菌和肠炎沙门氏菌的抑菌效果。结果表明,薄荷精油对蜡样芽孢杆菌、金黄色葡萄球菌和阪崎克罗诺杆菌具有一定的抑菌活性;然而,它并没有抑制肠炎沙门氏菌的生长。这表明薄荷油对革兰氏阳性菌和革兰氏阴性菌均有效。因此,在本研究中,薄荷油被证明具有很强的抗菌活性;它具有多种生物活性,可作为一种潜在的食品添加剂,用于改善各种乳基产品的质量。未来的研究应该在功能性乳制品中添加薄荷油,以预防和/或减轻特定疾病。
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引用次数: 7
Enhancement of Viability of Weissella cibaria CMU by Low-Temperature Encapsulation 低温包封法提高魏氏菌CMU活力的研究
Pub Date : 2018-09-01 DOI: 10.22424/JMSB.2018.36.3.171
Da-Eon Lee, Hyo-Jeong Hwang, Y. Jo, Tae-Hwan Jung, Kyoung-Sik Han
In this study, we aimed to investigate the effects of low-temperature encapsulation on the viability of Weissella cibaria CMU under harsh conditions and in freeze-dried foods during storage. The capsules were prepared by gelation of sodium caseinate at different concentrations (5%, 6%, 7%, and 8%) with a combination of 0.5% gellan gum and 2% glucono-δ -lactone. The size distribution of the capsules was determined using a Mastersizer 3000 laser diffraction particle size analyzer. Scanning and transmission electron microscopy revealed that the capsule with 6% sodium caseinate had a smooth and rounded external surface, with reproducibility. The acid, bile, and heat tolerances of the encapsulated cells were significantly higher than those of the control under prolonged acid (5 h), bile (12 and 24 h), and heat (2 h) exposure, respectively. During storage for up to 6 months at 4°C or 25°C, the viability of encapsulated Weissella cibaria CMU in beef and vegetable rice porridge was effectively improved.
在本研究中,我们旨在研究低温包封对魏氏菌(Weissella cibaria) CMU在恶劣条件下和在冻干食品中储存期间的活力的影响。将不同浓度(5%、6%、7%和8%)的酪蛋白酸钠与0.5%结冷胶和2%葡萄糖-δ -内酯混合制成胶囊。采用Mastersizer 3000激光衍射粒度分析仪测定胶囊的粒度分布。扫描电镜和透射电镜显示,添加6%酪蛋白酸钠的胶囊外表面光滑圆润,重现性好。长时间酸暴露(5 h)、胆汁暴露(12 h和24 h)和热暴露(2 h)下,包被细胞的耐酸性、胆汁耐受性和耐热性均显著高于对照组。在4°C或25°C的条件下,在长达6个月的时间里,包封的西巴尔魏氏菌(Weissella cibaria CMU)在牛肉和蔬菜米粥中的活力得到了有效提高。
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引用次数: 0
Sensory Evaluation of Various Gouda Cheeses Produced from Raw Milk 各种原料奶制成的豪达奶酪的感官评价
Pub Date : 2018-06-01 DOI: 10.22424/jmsb.2018.36.2.95
Cheol Choi, Hyun-Woo Lim, Jung-Whan Chon, Dong‐Hyeon Kim, K. Song, Se-Hyung Kim, Hyunsook Kim, K. Seo
Since 2018, the Korean government has permitted the production and sale of any cheese made from raw milk and aged for at least 60 days. The present study aimed to investigate the sensory characteristics of various types of Gouda cheese produced from raw milk during 60-day aging. The average pH of the raw milk used in this experiment was 6.7±0.4, similar to that of the sterilized milk. The pH of raw-milk Gouda cheese was 5.2±0.5 a day after the 60-day aging period, wherein the pH was 5.5±0.3. In total, 5 samples were used for sensory evaluation in this experiment: Cheeses 1∼5. With respect to flavor, Cheeses 1 and 5 received the highest and lowest scores, respectively. Raw-milk Gouda cheese produced using Salmonella spp.-contaminated raw milk was not greatly influenced by flavor; however, it had a negative effect on its appearance. In the future, it is imperative to carry out sensory evaluation of cheese produced with raw milk contaminated with various food-contaminating bacteria.
从2018年开始,韩国政府允许生产和销售任何由生牛奶制成的、陈年至少60天的奶酪。本研究旨在研究各种原料奶制成的豪达奶酪在60天陈化过程中的感官特性。本实验所用原料奶的平均pH值为6.7±0.4,与灭菌乳的pH值相近。经过60天的陈化期,生乳豪达奶酪的pH值为5.2±0.5 / d,其中pH值为5.5±0.3。在本实验中,总共有5个样品用于感官评价:奶酪1 ~ 5。在风味方面,奶酪1和5分别获得了最高和最低的分数。用沙门氏菌污染的原料奶生产的豪达奶酪风味受影响不大;然而,它对其外观产生了负面影响。今后,对受各种食品污染细菌污染的原料奶生产的奶酪进行感官评价势在必行。
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引用次数: 3
Entrapment of Ellagic Acid in Dairy Protein-Based Nanoparticles 鞣花酸在乳蛋白纳米颗粒中的包封
Pub Date : 2018-06-01 DOI: 10.22424/JMSB.2018.36.2.121
Mee-Ryung Lee
Ellagic acid (EA) is a naturally occurring polyphenolic compound in vegetables, nuts, and fruits such as berries. EA has antioxidant, anticancer, anti-allergy, and anti-inflammatory activities. The objectives of this research were to investigate the physicochemical properties of nanoparticles before and after nano-encapsulation of EA in dairy protein and to develop a functional (anti-inflammatory) dairy protein-based beverage containing EA. A particle size analyzer was used to determine the physicochemical and morphological properties. High performance liquid chromatography was used to evaluate the entrapment efficiency of EA. The nanoparticles containing EA were 100 to 200 nm in diameter. The determined poly dispersity index value of 0.3 to 0.4 indicated that the nanoparticles were uniformly distributed with similar size. Zeta-potential values were also similar between the control groups. The entrapment efficiency of EA was nearly 90%. The results indicate the potential for development of nanoparticles containing EA beverage products with antiinflammatory activity.
鞣花酸(EA)是一种天然存在的多酚化合物,存在于蔬菜、坚果和浆果等水果中。EA具有抗氧化、抗癌、抗过敏和抗炎活性。本研究的目的是研究乳蛋白中EA纳米包封前后纳米颗粒的物理化学性质,并开发一种含有EA的功能性(抗炎)乳蛋白饮料。采用粒径分析仪测定了EA的物理化学和形态性质。采用高效液相色谱法对EA的包封效率进行了评价。含EA的纳米颗粒直径为100 ~ 200 nm。测定的多分散性指数值为0.3 ~ 0.4,表明纳米颗粒分布均匀,粒径相近。泽塔电位值在对照组之间也相似。EA的捕集效率接近90%。研究结果表明,具有抗炎活性的含EA纳米颗粒饮料产品具有开发潜力。
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引用次数: 1
Chemical Properties of Lignans, Their Effects on Human Health, and the Enhancement of Milk Function of Lignans 木脂素的化学性质、对人体健康的影响及对乳功能的增强
Pub Date : 2018-06-01 DOI: 10.22424/JMSB.2018.36.2.81
Hyo-Jeong Hwang, Jin A Yoon, K. Shin
This review discusses the characteristics of major lignans and related studies and provides a basis for future studies. Lignans are present in various food products consumed daily, such as flaxseed and other seeds, vegetables, fruits, and beverages including coffee, tea, and wine. Lignans are natural phytoestrogens with a structure similar to that of secoisolariciresinol (Seco), mataireinol (Mat), pinoresinol (Pin), medioresinol (Med), lariciresinol (Lari), and syringaresinol, which is then converted to enterodiol (END) and enterolactone (ENL), which are mammalian lignans and the primary biologically active enterolignans, by the intestinal microflora. The associations between lignans and a decreased risk of cardiovascular disease are promising; however, they are not yet well-established, probably owing to low lignan intake in habitual Western diets. Nonetheless, these associations were more prominent at the higher doses in interventional studies. Many studies on humans and animals have reported the benefits of lignan consumption in protecting against CVD and metabolic syndrome by reducing lipid and glucose concentrations. END and ENL reportedly exert protective effects including phytoestrogenic, antioxidant, anti-inflammatory, and anticancer effects through various mechanisms. Moreover, lignans reportedly exert beneficial effects in breast, colon, and prostate cancer and osteoporosis have reported that. However, future studies are required to confirm the association between lignan and disease.
本文综述了主要木脂素的特点及相关研究,为今后的研究提供基础。木脂素存在于日常食用的各种食品中,如亚麻籽和其他种子、蔬菜、水果和饮料,包括咖啡、茶和葡萄酒。木脂素是一种天然植物雌激素,其结构类似于二异松脂醇(Seco)、松脂醇(Mat)、松脂醇(Pin)、中松脂醇(Med)、松脂醇(Lari)和丁香松脂醇,然后被肠道菌群转化为肠二醇(END)和肠内酯(ENL),它们是哺乳动物的木脂素和主要的生物活性肠脂素。木脂素与降低心血管疾病风险之间的联系是有希望的;然而,它们还没有建立起来,可能是由于习惯西方饮食中木脂素摄入量低。尽管如此,在介入研究中,这些关联在高剂量时更为突出。许多对人类和动物的研究已经报道了木脂素的摄入通过降低脂质和葡萄糖浓度来预防心血管疾病和代谢综合征的益处。据报道,END和ENL通过多种机制发挥保护作用,包括植物雌激素、抗氧化、抗炎和抗癌作用。此外,据报道木脂素对乳腺癌、结肠癌、前列腺癌和骨质疏松症都有有益的作用。然而,需要进一步的研究来证实木脂素与疾病之间的联系。
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引用次数: 2
Objective Measurements of Textural and Rheological Properties of Cheese 奶酪结构和流变特性的客观测量
Pub Date : 2018-06-01 DOI: 10.22424/JMSB.2018.36.2.73
Mee-Ryung Lee
The textural and rheological properties of cheese are major attributes for the characterization of cheese types, ripening, and consumer preferences. The use of small amplitude oscillatory rheological testing has made it possible for cheese researchers to assess the major properties of cheese, such as melting behavior and storage modulus, without irreversible deformation. In addition, large deformation testing such as textural profile analysis can assess properties such as hardness of cheese. While the sensory properties of cheese are valued by consumers, objective and reliable measurements are paramount for researchers. Ongoing development and refinement of scientific measurement methods of cheese are vital.
奶酪的质地和流变特性是决定奶酪种类、成熟度和消费者偏好的主要属性。使用小振幅振荡流变测试使奶酪研究人员能够评估奶酪的主要特性,如熔化行为和储存模量,而不会产生不可逆的变形。此外,大变形测试,如纹理剖面分析,可以评估奶酪的硬度等特性。虽然消费者重视奶酪的感官特性,但对研究人员来说,客观可靠的测量是至关重要的。不断发展和完善科学的奶酪测量方法是至关重要的。
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引用次数: 3
Microbiological Safety of Various Gouda Cheeses Produced from Raw Milk 各种原料奶制成的豪达奶酪的微生物安全性
Pub Date : 2018-06-01 DOI: 10.22424/jmsb.2018.36.2.106
Cheol Choi, Dong‐Hyeon Kim, Hyun-Woo Lim, Jung-Whan Chon, K. Song, Se-Hyung Kim, Hyunsook Kim, K. Seo
Since 2018, the production and sales of ram-milk cheese ripened for over 60 days has been permitted in South Korea. Hence, this study aimed to examine the microbiological changes in 7 different types of Gouda cheese. During the aging period, traditional raw-milk Gouda Cheeses 1 and 2 did not contain Salmonella spp. during the 60-day storage period and no E. coli after 20-day storage. Coliform bacteria were not detected in Cheese 1 after 40 days; however, they were detected in Cheese 2 up to 60 days. Salmonella spp. were inhibited during the 60-day storage period in Cheese 3 (Salmonella spp.-contaminated raw-milk Gouda cheese), Cheese 4 (Cheese 3 contaminated with lactic acid bacteria DH 5 isolated from Kefir) and Cheese 5 (Cheese 3 contaminated with lactic acid bacteria DN1 isolated from Kefir). In particular, inhibition of Salmonella spp. was more prominent in Cheese 4 and Cheese 5 than in Cheese 3. During 60-day storage, Cheese 6 had a significantly reduced lactic acid bacteria. Furthermore, in Cheese 7, E. coli, E. Salmonella ssp. were rarely detected, and lactic acid bacteria were slightly greater in Cheese 7 than in other cheeses during the 60-day period. Moreover, all samples from Cheese 1 to Cheese 7 were not contaminated with Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, and E. coli O157:H7.
从2018年开始,韩国允许生产和销售成熟60天以上的公羊奶奶酪。因此,本研究旨在研究7种不同类型的豪达奶酪的微生物变化。在陈化过程中,传统生乳豪达奶酪1号和2号在60天的贮存期内未发现沙门氏菌,在20天的贮存期内未发现大肠杆菌。40 d后,奶酪1中未检出大肠菌群;然而,在奶酪2中检测到它们长达60天。在60 d的贮藏期内,奶酪3(沙门氏菌污染的生乳Gouda奶酪)、奶酪4(奶酪3污染的乳酸菌DH - 5)和奶酪5(奶酪3污染的乳酸菌DN1)对沙门氏菌均有抑制作用。其中,奶酪4和奶酪5对沙门氏菌的抑制作用明显强于奶酪3。在贮藏60 d期间,6号奶酪的乳酸菌数量显著减少。此外,在奶酪7中,大肠杆菌,沙门氏菌。在60天的时间里,奶酪7中的乳酸菌略高于其他奶酪。奶酪1 ~奶酪7样品均未检出单核增生李斯特菌、金黄色葡萄球菌、产气荚膜梭菌和大肠杆菌O157:H7。
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引用次数: 1
Determination of Microbial Diversity in Gouda Cheese via Pyrosequencing Analysis 高达干酪微生物多样性的焦磷酸测序分析
Pub Date : 2018-06-01 DOI: 10.22424/JMSB.2018.36.2.125
Sangnam Oh, Younghoon Kim
The present study aimed to investigate the microbial diversity in Gouda cheese within the four months of ripening, via next-generation sequencing (NGS). Lactococcus (96.03%), and Leuconostoc (3.83%), used as starter cultures, constituted the majority of bacteria upon 454 pyrosequencing based on 16S rDNA sequences. However, no drastic differences were observed among other populations between the center and the surface portions of Gouda cheese during ripening. Although the proportion of subdominant species was <1%, slight differences in bacterial populations were observed in both the center and the surface portions. Taken together, our results suggest that environmental and processing variables of cheese manufacturing including pasteurization, starter, ripening conditions are important factors influencing the bacterial diversity in cheese and they can be used to alter nutrient profiles and metabolism and the flavor during ripening.
本研究旨在通过下一代测序(NGS)研究豪达奶酪成熟4个月内的微生物多样性。基于16S rDNA序列的454磷酸测序结果显示,乳球菌(96.03%)和Leuconostoc(3.83%)作为发酵剂,占细菌总数的大多数。然而,在其他种群中,在成熟过程中,豪达奶酪的中心部分和表面部分没有明显差异。虽然亚优势种的比例小于1%,但在中心和表面部分都观察到细菌种群的微小差异。综上所述,我们的研究结果表明,奶酪生产过程中的环境和加工变量(包括巴氏灭菌、发酵剂、成熟条件)是影响奶酪细菌多样性的重要因素,它们可以用来改变奶酪成熟过程中的营养成分、代谢和风味。
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引用次数: 1
期刊
Journal of Milk Science and Biotechnology
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