Entomological Research最新文献

Recent reports have disclosed the occurrence of Wolbachia in Aedes aegypti. Our study detected Wolbachia infection in Ae. aegypti by screening wild adult mosquitoes using two Wolbachia-specific molecular markers. Overall, 444 adult Ae. aegypti mosquitoes were collected from April 2022 to October 2022 in Jeddah, Saudi Arabia. Each individual sample was processed and screened for the presence of Wolbachia using selected markers, the Wolbachia-specific 16S rDNA and the Wolbachia surface protein gene (wsp), under optimized polymerase chain reaction (PCR) conditions, and sequenced. In total, 39 (8.78%) and 48 (10.81%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. By utilizing two Wolbachia-specific molecular markers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low rate of Wolbachia infection and inferred that the detected strain belongs to supergroup B.

Alam A, Abbas S, Ali J et al. (2024) Diet suitability through biological parameters in Ostrinia furnacalis (Lepidoptera: Crambidae) clades. Entomological Research 54(6), e12751.

In the published article, the ‘Author Contribution’ section was incorrect. This should have read:

Aleena Alam: Conceptualization, Methodology, Formal analysis, Writing— original draft preparation, and Writing— review & editing. Sohail Abbas, Wang Liangzhu, Feng Xiao, QinWeibo, Wu Haichao, Liu Jiali, Arzlan Abbas: Data curation and Writing— review & editing. Jamin Ali, Muhammad Shakeel, Farman Ullah, Khalid Ali Khan, Hamed A. Ghramh: Writing— review & editing. Xie Zhiming: Funding acquisition. Chen Ri Zhao: Conceptualization, Supervision, Validation, Visualization, Resources, Project administration, Methodology, Writing-review & editing and Funding acquisition. All authors have read and agreed to the published version of the manuscript.

We apologize for this error.

Ensemble species distribution modeling offers a robust approach to reduce the inherent uncertainties associated with single models, and ultimately providing more accurate predictions of regions with a heightened probability of occurrence. As Corythucha ciliata (Say) damages deciduous trees in diverse environments, including urban, suburban and forested regions, the objective of this study was to predict the potential distribution of C. ciliata by developing an ensemble model that comprehensively considered the biological and habitat traits of the pest using the CLIMEX and MaxEnt models. Although the ensemble model did not have significantly improved performance, compared with the single MaxEnt model, it was robust compared with distribution data. Our predictions suggest that C. ciliata will gradually expand its range from its current distribution in response to climate change, necessitating focused monitoring and pest-control efforts in the predicted regions. This study not only evaluates pest distribution but also provides crucial insights into effective control strategies, which are adaptable to other pest management scenarios.

To improve the prediction accuracy of Dendrolimus punctatus larvae of overwintering, first and second generation and to provide a scientific basis for effective prevention and control of D. punctatus, six mathematical models of fuzzy comprehensive evaluation method were used to predict accumulative amount with the data of 1989–2014. The cumulative amount for the overwintering and the first generation was predicted in 2015, 2016, 2017, and 2018. The forecasted results of six models were all level 1, which was consistent with the actual results. Five forecasted results of six models and the actual results were level 1. The actual accumulative amount of D. punctatus for the second generation was level 1 in 2015, 2016, and 2017, which were consistent with the six models' forecasted results.

The Asian corn borer (ACB), Ostrinia furnacalis, (Lepidoptera: Crambidae), a significant threat to corn crops, necessitates comprehensive research on its populations, across multiple clades. This study addresses the efficacy of various diets, such as an antibiotic mixed diet of amoxicillin mixed with artificial diet, wheat germ flour artificial diet and fresh corn leaves, for the successful mass rearing of three clades of O. furnacalis, and investigates variations among the insects reared under laboratory conditions. We validate these findings for O. furnacalis clades using biological attributes. Notably, clade III demonstrated enhanced biological characteristics with an antibiotic mixed diet, in contrast to clades I and II, with higher growth indexes observed during mass rearing. Through revealing a suitable diet for the clade, this research advances our understanding of O. furnacalis dynamics and offers valuable insights for practical applications in an integrated pest management program.

Recent studies have suggested the use of insects to enhance various bioactivities in the treatment of many diseases or as alternative sources of nutrients. White-spotted flower chafer (Protaetia brevitarsis seulensis; PBS) extract, which is used for medicinal purposes, appears to be effective in improving blood circulation. However, few studies have investigated the efficacy of PBS extract in improving blood circulation. Here, we investigated the effects of PBS extract on serum cholesterol metabolites (total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol) and anticoagulant activities (prothrombin time [PT], activated partial thromboplastin time [aPTT], and fibrinogen [Fib]) in very-high-fat diet (VHFD)-induced hyperlipidemic rats. The experimental groups comprised a control group that was not treated with PBS and five PBS treatment groups (50, 100, 150, 200, and 250 mg/kg) in a randomized complete block design. In the efficacy evaluation for health functionality to reduce cholesterol levels, no beneficial effects on serum cholesterol metabolites were observed in the control and PBS treatment groups. In addition, the cholesterol data indicated that the effect of the PBS extract was not related to antioxidant activity. Moreover, the PT, aPPT, and Fib values were prolonged by increasing the PBS extract concentration, and PBS extract concentrations of 150–250 mg/kg were recommended for the VHFD model based on the anticoagulant activity. In conclusion, the PBS extract can be used in a potential therapeutic strategy for improving anticoagulant activity rather than improving the serum cholesterol profile.

Pardosa pseudoannulata is key predator in rice ecosystems; it plays a crucial role in rice pest biological control, effectively managing major rice pests such as rice planthopper. At present, there are few related studies on spiders' microRNA (miRNA). Consequently, we initiate a study on miR-6826-3p in P. pseudoannulata in response to high temperatures. First, we identified a total of 686 target genes of miR-6826-3p, 439 target genes had annotated results in the database, primarily involved in transcription and metabolism functions. Subsequently, quantitative real-time PCR was employed to assess the relative expression levels of miR-6826-3p in P. pseudoannulata under different temperatures (31, 34, 37, 40°C) and various durations (1, 2, 4, 8, 12 h). The results revealed that compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased under the same temperature and different duration stresses. The expression level of miR-6826-3p in male spiders showed an initial increase followed by a decrease trend, with relatively lower expression levels after 1 and 12 h at the same temperature conditions. Furthermore, under the same duration of stress, compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased at different temperatures. These results demonstrate that P. pseudoannulata miR-6826-3p plays a significant role in response to high-temperature stress. The research findings can provide a basis for future in-depth studies on the miRNA functional of P. pseudoannulata, aiding in the elucidation of the regulatory mechanisms of P. pseudoannulata miRNAs in response to high-temperature environments.

To identify viruses and compare their abundance levels in the venom glands of hymenopteran species, we conducted venom gland-specific transcriptome assemblies and analyses of 22 aculeate bees and wasps and identified the RNA genomes of picornaviruses. Additionally, we investigated the expression patterns of viruses in the venom glands over time following capture. Honeybee-infecting viruses, including the black queen cell virus (BQCV), the deformed wing virus (DWV) and the Israeli acute paralysis virus (IAPV), were highly expressed in the venom glands of Apis mellifera and social wasps. This finding suggests that the venom of bees and wasps is likely to contain these viruses, which can be transmitted horizontally between species through stinger use. Apis mellifera exhibited an increasing pattern of abundance levels for BQCV, DWV, IAPV and Triatovirus, whereas the social wasp Vespa crabro showed increasing abundance levels of IAPV and Triatovirus over different capture periods. This suggests that the venom glands of honeybees and wasps may provide suitable conditions for active viral replication and may be an organ for virus accumulation and transmission. Some viral sequences clearly reflected the phylogeny of aculeate species, implying host-specific virus evolution. On the other hand, other viruses exhibited unique evolutionary patterns of phylogeny, possibly caused by specific ecological interactions. Our study provides insights into the composition and evolutionary properties of viral genes in the venom glands of certain aculeate bees and wasps, as well as the potential horizontal transmission of these viruses among bee and wasp species.

Edible insects such as the mealworm (Tenebrio molitor) are emerging as an alternative animal protein resource to meet the needs of a growing global population. However, despite the successful mass production of edible insects, research into practical compound feed for edible insect is still insufficient. In this study, mealworms were fed wheat bran, layer chicken feed mash, chicken pellets and cattle pellets for 1 to 36 days, after which their body length was measured. In the groups fed with chicken feed mash, chicken pellets or cattle pellets, the mean body lengths were 2.88, 2.92 and 2.95 cm, respectively, similar to the 2.95 cm of the control T. molitor fed with wheat bran. Individual weight measurements yielded similar results: control, 7.87 g; chicken feed mash, 7.43 g; chicken pellets, 7.60 g; and cattle pellets, 7.63 g. Analysis of livestock feed intake showed that the intake was 290.4 g in control insects, 137.7 g in insects fed chicken feed mash, 220.2 g in insects fed chicken pellets and 230.3 g in insects fed cattle pellets. In the excretion analysis, the excretion of the control insects (161.60 g) was larger than that of the insects fed with chicken feed mash (93.37 g), chicken pellets (93.40 g) or cattle pellets (133.87 g). An analysis of the nutritional content of T. molitor based on nine major nutrients showed no significant differences between the control and the test groups. These results can be used to improve the price competitiveness and quality of T. molitor for commercial development.

Bactrocera dorsalis (Hendel), a very destructive insect pest of many fruits and vegetables, is widespread in the tropical and subtropical zones of Asia. However, the northward extension of B. dorsalis has been reported in China recently. It is crucial to determine the origin of new populations and to investigate the invasive potential of the species. Here, we investigated B. dorsalis in Nanyang, Henan Province and Heze, Shandong Province using a combined methodological approach. We first detected the hydrogen stable isotope ratios (δ²H) of B. dorsalis samples. The results showed that the δ²H values for B. dorsalis from Nanyang were consistent with those of the local rainwater. In contrast, the samples from Heze were inconsistent with those of the rainwater in Heze. We then sequenced the complete mitochondrial cytochrome oxidase I (mtDNA COI) from the same fruit fly samples for haplotype diversity and phylogenetic analyses. We found a close genetic relationship between the Heze population and Nanyang, Hefei, Sanya, Fuyang and Wuhan populations. Besides, we surveyed the fruit's origin in the wholesale market, and concluded that B. dorsalis trapped in Heze might have come from Hainan and Zhejiang in China. Finally, overwintering potential experiments showed that a small number of pupae could survive during the test periods in Nanyang, whereas no pupae could survive in Heze. Overall, this study reveals that the Nanyang population of B. dorsalis were resident, whereas the Heze population did not colonize and were repeated entries from other areas every spring.