Entomological Research最新文献

Honey, which is medicinally very important, is the major product of honeybees. The role of the honeybee queen is crucial in maintaining the health and stability of the colony as it is responsible for reproducing and maintaining the population of the hive. Productive and healthy honeybee queens ensure the success of the colony. Various factors, such as parasites, diseases, lack of food and habitat, climate change, genetic defects, and exposure to pesticides, can lead to the failure of the queen bee. Therefore, applications of artificial queen-rearing techniques in beekeeping positively affect the growth of honeybee queens with desirable characteristics. This current research was conducted at the KUST Model Bee Form of Kohat University of Science and Technology, on Apis mellifera honeybee colonies during spring 2021. In this study, the Doolittle grafting method was used for the rearing of the A. mellifera queen bee. A total of 230 larvae of different ages (12–24, 24–48, and 48–72 h old larvae were used for grafting. Of these 230 grafted larvae, the number of queen cup cells accepted was 139 and the number of emerged queen cup cells was 82. The queen cell acceptance rate was significant (P = 0.000; P < 0.05). The effect of larval age, and the addition of royal jelly in queen cup cells prior to grafting, on the acceptance rate of queen cup cells was also studied. Young larvae, that is, from 12–24 up to 48 h of age, showed high acceptance rates. The acceptance rates of 12–24, 24–48 and 48–72 h old larvae were 76.7%, 55.7% and 20.0%, respectively. This shows that a queen produced from a 1 day old larva has the highest acceptance rate. The addition of royal jelly into queen cup cells before grafting showed the highest larval acceptance rate in March and April. In this study the acceptance rate for queen cup cells with wet and dry grafting were 89.5% and 46.1%, respectively. However further studies are recommended to determine the effect of all factors on the morphology of the queen.

The Korea Combat Training Center (KCTC), located in Gangwon Province, is a restricted military training facility where research on the environmental conditions and health risks to military personnel has been limited. In this study, using iSeq 100, we investigated the bacterial abundance and microbiome of Haemaphysalis longicornis specimens collected at the KCTC from June to August 2022, to assess current and potential public health risks to military personnel. Our results show that adult ticks had significantly greater species richness compared with larvae and nymphs, with no notable differences in diversity across developmental stages. Principal coordinate analysis of the microbial communities did not show differences attributable to any single factor, such as collection location or date. Coxiella-like endosymbionts (AB001519) were identified in all 13 samples, and Jatrophihabitans, Sphingomonas, and Spirosoma were consistently found across all samples. In addition, iSeq 100 also identified Rickettsia rickettsii and Borrelia spp., which were not detected with conventional polymerase chain reaction (PCR).

This research summarizes the data on benthic macroinvertebrates collected in the 15 streams during spring (June) and fall (September–October) of 2022. The objectives of this study were (i) to evaluate the quality of surface waters, sediments, and benthic macroinvertebrates; and (ii) to compare the relationship with the environmental variables in water, sediments and benthic macroinvertebrates that inhabit 15 streams. A total of 114 taxa were collected. The assemblages from the 15 sites were dominated by Chironomidae and Tubificidae, especially Chironomidae constituted 45.0% density occurrence. The low grades of Benthic Macroinvertebrate Index (BMI) were calculated in 10 sites with disturbed (“poor” or “very poor”). Low BMI sites mostly showed Tubificidae or Chironomidae dominant characteristics. The biological indices, BMI, % Tubificidae, and % Chironomidae, were significantly correlated with DO and conductivity. DO was also significantly correlated with all the benthic macroinvertebrate factors and diversity except percent Tubificidae. The heavy metal parameters were correlated mostly with species number, BMI, and diversity index. The objectives of this study examine the diversity of benthic macroinvertebrate in the 15 streams and investigate the relative importance of environmental quality for benthic macroinvertebrate distribution.

Malaria, a globally significant mosquito-borne infectious disease, re-emerged in the Republic of Korea, and manifested annually in regions close to the demilitarized zone. Notably, Incheon Metropolitan City has witnessed an alarming upswing in malaria infections in recent years, drawing attention to this public health issue. This research was conducted to catch spatio-temporal and ecological landscape encompassing malaria patients and mosquito vectors in Incheon over the past decade. The top two incidences of malaria cases were found in Ganghwa-gun and Seo-gu, an occurrence potentially attributed to their geographic proximity to North Korea. Furthermore, the incidence of malaria infections displayed a seasonal pattern commencing in March, reaching its peak between June and August, and decreasing to a minimum in November. A correlation was noted between prevalence of malaria cases and number of mosquito breeding sites, such as ponds and rice fields within the region. Collectively, these research outcomes underlined the importance of systematically and holistically advocating mosquito elimination measures to enhance the efficacy of malaria eradication policies. These measures encompass the establishment of a robust mosquito outbreak surveillance system, targeted control of vector mosquitoes, residual pesticide spray, management of mosquito breeding sites, and adoption of repellents during outdoor activities.

In recent years, the study of insect flight behavior has gained prominence, offering insights into their dispersal strategies, habitat preferences, and potential migration patterns. Recognizing the need for a precise tool to capture insect flight, we developed an Arduino-integrated flight mill that offers researchers affordability, accuracy, and adaptability. Our flight mill was rigorously tested on two insect species, Protaetia brevitarsis seulensis and Polygonia c-aureum, and proved effective in recording their unique flight patterns. Key to the precision of our flight mill is the integration of advanced materials, such as polytetrafluoroethylene, which reduces friction, and the incorporation of magnetic sensing for meticulous data capture. These design choices ensure that the natural flight speed of the insect is replicated with minimal disturbance. Additionally, the implementation of a dedicated R script for data analysis provides researchers with a comprehensive platform, allowing them to delve deeply into various metrics, visualize patterns, and make informed conclusions. However, our flight mill is not without challenges. Mastery of the Arduino platform and R programming environment is essential for optimal use, which could pose barriers for those unfamiliar with these platforms. Moreover, although carefully designed, the mounting mechanism may still influence the natural behavior of the insect. Despite these challenges, the potential applications of our flight mill are myriad. From basic entomological research to practical applications in pest management and conservation, its versatility is evident. The adaptability of the tool also makes it an excellent resource in educational settings, offering students hands-on experience in studying insect behavior.

This study was conducted to demonstrate the effect of the mass trapping of mosquitoes as an eco-friendly physical control method. It was conducted at the Ansan Hwarang Auto Camping Site in Gyeonggi Province, Republic of Korea. The study period was approximately 1 month (from July 2 to July 30, 2018). Inner and outer traps were installed to control mosquitoes inside and outside the study area. The inner trap consisted of 5 digital mosquito monitoring system units placed inside the study area, whereas the outer trap included 26 MOSHOLE-PRO units installed at 30 m intervals outside the study area. Both the inner and outer traps used carbon dioxide as an attractant. Additionally, the outer traps were grouped close to each inner trap using distance analysis and designated as Sn (n, inner trap number). According to the study results, analysis of the daily mean temperature and daily mean precipitation from 2017 to 2019 showed that the meteorological data were statistically insignificant. During the mass trapping period, the outer traps had the highest mean number of mosquitoes in the S1 group, whereas the other groups exhibited statistically similar results. Analysis of the mean number of mosquitoes in the inner trap confirmed a mean reduction rate of 59.39% through mass trapping, and the results were statistically significant (P < 0.001). This study confirms the effectiveness of mass trapping in reducing mosquito communities and suggests the possibility of creating mosquito-free zones by targeting mosquitoes both inside and outside the study area.

As Aedes mosquitoes are the predominant carriers of arboviruses responsible for global dengue fever and chikungunya outbreaks, understanding their genetic diversity and population structure can enhance dengue prevention and vector control efforts. Although sympatric cryptic species have been acknowledged in Aedes species subgroups in Southeast Asia, little information is available regarding the occurrence and dispersal of cryptic Aedes species in Saudi Arabia. In the present investigation, we intend to analyze genetic variations and perform a phylogenetic study of sympatric Aedes samples collected from various locations in Taif Province, Saudi Arabia. The locus of mitochondrial DNA cytochrome c oxidase subunit 1 (COX1, analyzed with DNA barcoding) was utilized to determine genetic variance and to build phylogenetic trees. For species identification, the COX1 sequences of Aedes samples from Taif Province were compared with those of Aedes samples from GenBank (National Center for Biotechnology Information, NCBI). To identify phylogenetic relationships and genetic variability, phylogenetic trees were created using Taif Province samples, GenBank A. albopictus samples, and GenBank A. aegypti samples. The phylogenetic analysis revealed that some Taif samples (A3, A7, F2, F4, F1, A4, A1, F9 and F6) were closer to A. aegypti and A. albopictus samples from Africa and East Asia, especially Kenya and Malaysia. Whereas other samples (F9, F6, A4, F1 and A1) were closer to the GenBank A. aegypti samples KP843383 (Thailand), HM399357(Australia), MK533632 (Kenya), KX420460 (Kenya), KU495081 (Australia) and MF179160 (China) than the remaining Taif samples. Overall, our findings demonstrate that examining the genetic diversity and phylogenetic linkages of Aedes samples can aid in understanding the evolution of Aedes cryptic species in the western regions of Saudi Arabia.

Butterflies are expedient biodiversity indicators of a healthy environment. As virtuous pollinators, they provide environmental and economic benefits to the agricultural and natural ecosystem. The global loss of the butterfly community, especially in tropical regions, is causing irreparable damage to the ecological landscape. This decline in population is due to habitat degradation, forest succession, loss of symbiotic relations, economic loss due to flooding, forest development plans, low grassland area, global warming, and reforestation. Moreover, the limited information on the molecular resources of butterflies impedes informed conservation planning. The Threatened Species Initiative (TSI) of Korea has started to utilize the benefits of transcriptome-guided discovery of molecular resources of a few butterfly species, and a few others are in the pipeline. The cataloging of new genes and genetic markers provides the possibility to promote selective utilization of fitness traits for adaptation of the species to the wild and a newer habitat. In this review, we assessed the versatility of whole-transcriptome information of the Korean threatened butterfly species, such as the lycaenid Protantigius superans and Spindasis takanosis, the Nymphalid butterfly, Fabriciana nerippe, and the Papilionid species Parnassius bremeri. The schema employed under TSI, including the sequencing and downstream bioinformatics pipeline, could act as a reference for research outside of Korean butterflies. While the molecular resources are available to be exploited, their utilization for conservation is still in its infancy. It is important to synthesize these studies together to catalyze further conservation work applying genomics approaches.

The poultry industry needs alternative feeds with no adverse effects on animal performance and egg quality. Insects represent effective animal feeds. However, Ptecticus tenebrifer (PT) has rarely been studied as an effective feed in terms of fatty acid contents. Therefore, we investigated the effect of dietary PT powder on the fatty acid profiles of egg yolks in laying hens. A total of 180 Hy-Line brown hens were divided equally into three groups (n = 60), with three replicates: the control group and two groups fed 2% and 4% PT powder, respectively. After 2 weeks, minor effects (P < 0.05) on oleic acid, dihomo-gamma-linolenic acid, and total monounsaturated fatty acids were observed among the groups. After 4 weeks, docosahexaenoic acid, docosapentaenoic acid, myristic acid, oleic acid, and total polyunsaturated fatty acids were slightly altered in the treatment groups (P < 0.05). For other fatty acids, the 2% and 4% PT groups had fatty acid concentrations similar to those of the controls. No differences in total n-6 and total n-3 were observed among the groups. In conclusion, using 2% and 4% PT powders did not notably change the fatty acid profiles of egg yolks and could replace some layer-hen feed without negative effects.

This study examined the effects of various insect species, such as those of Bombyx mori, Hermetia illucens and Tenebrio monitor, that have long been used in combination with medicinal plants and are an important alternative to modern medicine in many parts of the world. The current use of traditional pesticides from around the year 2010 and the characteristics of isolated biologically active molecules are also presented. Natural insect products were examined or isolated from ants, bees, beetles, cockroaches, termites, bees, etc. The biological activities of these natural products derived from insects include antimicrobial, antifungal, anticancer, antiviral, antioxidant and anti-inflammatory effects. Insect additives to animal feed rarely distort the relevant physicochemical variables or sensory profile of the meat, whereas the fatty acid (FA) profile is discernibly changed. In fact, insect derivatives have great potential for future drug discovery in natural products, as well as for the production of nutritious foods for humans and for animals. It is concluded that the inoculation of insect powder in animal feeds results in the prevention and treatment of disease, enhances the growth of animals and promotes the bioactive compounds in animal-based products. Owing to the sustainability of their breeding, large-scale cultures of these insects seem economically viable. Insect oil and protein as well as chitin ingredients are widely used in cosmetics, and these substances are considered high-value co-products. The major challenges associated with their use will be linked to the development of methods for separating industrial-derived materials.