首页 > 最新文献

Equine veterinary journal. Supplement最新文献

英文 中文
Adipose tissue-derived adiponectin expression is significantly associated with increased post operative mortality in horses undergoing emergency abdominal surgery. 在接受紧急腹部手术的马中,脂肪组织来源的脂联素表达与术后死亡率增加显著相关。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00404.x
M J Packer, A J German, L Hunter, P Trayhurn, C J Proudman

Reasons for performing study: Adipose tissue is an important source of inflammatory cytokines (adipokines) and adiposity has been identified as having a significant effect on human morbidity and mortality. Obesity is also an emerging welfare problem in the UK horse population, but the role that it plays in secondary diseases is unclear.

Objectives: To examine the expression of inflammation-related adipokine genes in retroperitoneal adipose tissue of horses undergoing emergency abdominal surgery and to explore associations with adiposity and post operative survival.

Methods: Retroperitoneal adipose tissue samples were obtained from 76 horses undergoing emergency abdominal surgery. Real-time PCR was used to measure gene expression for leptin, adiponectin, tumour necrosis factor-alpha, macrophage chemoattractant protein-1, macrophage inhibitory factor, serum amyloid A, haptoglobin and interleukin-1. Multivariate patterns of adipokine expression were explored with principal component analysis (PCA), whilst univariable associations with post operative survival were tested in a Cox proportional hazards model.

Results: Leptin gene expression was higher in overweight and obese horses than in lean animals. Expression of mRNA encoding adiponectin mRNA in visceral adipose tissue was positively associated with increased post operative mortality (hazard ratio 1.31, 95% CI 1.05-1.65). However, PCA did not demonstrate multivariable patterns of adipokine gene expression from visceral adipose tissue associated with body mass index or with survival.

Conclusions: In horses presented with acute intestinal disease, increased adiponectin gene expression from retroperitoneal adipose tissue is associated with an increased risk of mortality. Obesity assessed by BMI had no association with increased post operative mortality in horses with primary gastrointestinal disease.

Potential relevance: Further study is warranted on the expression and effects of adipokines, particularly adiponectin, and correlation with postoperative outcome.

研究原因:脂肪组织是炎症细胞因子(脂肪因子)的重要来源,肥胖已被确定对人类发病率和死亡率有重要影响。肥胖也是英国马的福利问题,但它在继发性疾病中所起的作用尚不清楚。目的:检测急诊腹部手术马腹膜后脂肪组织中炎症相关脂肪因子基因的表达,并探讨其与肥胖和术后生存的关系。方法:选取76匹急诊腹部手术马腹膜后脂肪组织标本。采用Real-time PCR检测瘦素、脂联素、肿瘤坏死因子- α、巨噬细胞趋化蛋白-1、巨噬细胞抑制因子、血清淀粉样蛋白A、触珠蛋白和白细胞介素-1的基因表达。用主成分分析(PCA)探讨脂肪因子表达的多变量模式,同时用Cox比例风险模型检验与术后生存的单变量关联。结果:瘦素基因在超重和肥胖马中的表达高于瘦马。内脏脂肪组织中编码脂联素mRNA的mRNA表达与术后死亡率增加呈正相关(风险比1.31,95% CI 1.05-1.65)。然而,PCA并没有证明内脏脂肪组织中脂肪因子基因表达与体重指数或生存相关的多变量模式。结论:在患有急性肠道疾病的马中,来自腹膜后脂肪组织的脂联素基因表达增加与死亡风险增加有关。BMI评估的肥胖与原发性胃肠道疾病马术后死亡率增加无关。潜在相关性:需要进一步研究脂肪因子的表达和作用,特别是脂联素,以及与术后预后的关系。
{"title":"Adipose tissue-derived adiponectin expression is significantly associated with increased post operative mortality in horses undergoing emergency abdominal surgery.","authors":"M J Packer,&nbsp;A J German,&nbsp;L Hunter,&nbsp;P Trayhurn,&nbsp;C J Proudman","doi":"10.1111/j.2042-3306.2011.00404.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00404.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Adipose tissue is an important source of inflammatory cytokines (adipokines) and adiposity has been identified as having a significant effect on human morbidity and mortality. Obesity is also an emerging welfare problem in the UK horse population, but the role that it plays in secondary diseases is unclear.</p><p><strong>Objectives: </strong>To examine the expression of inflammation-related adipokine genes in retroperitoneal adipose tissue of horses undergoing emergency abdominal surgery and to explore associations with adiposity and post operative survival.</p><p><strong>Methods: </strong>Retroperitoneal adipose tissue samples were obtained from 76 horses undergoing emergency abdominal surgery. Real-time PCR was used to measure gene expression for leptin, adiponectin, tumour necrosis factor-alpha, macrophage chemoattractant protein-1, macrophage inhibitory factor, serum amyloid A, haptoglobin and interleukin-1. Multivariate patterns of adipokine expression were explored with principal component analysis (PCA), whilst univariable associations with post operative survival were tested in a Cox proportional hazards model.</p><p><strong>Results: </strong>Leptin gene expression was higher in overweight and obese horses than in lean animals. Expression of mRNA encoding adiponectin mRNA in visceral adipose tissue was positively associated with increased post operative mortality (hazard ratio 1.31, 95% CI 1.05-1.65). However, PCA did not demonstrate multivariable patterns of adipokine gene expression from visceral adipose tissue associated with body mass index or with survival.</p><p><strong>Conclusions: </strong>In horses presented with acute intestinal disease, increased adiponectin gene expression from retroperitoneal adipose tissue is associated with an increased risk of mortality. Obesity assessed by BMI had no association with increased post operative mortality in horses with primary gastrointestinal disease.</p><p><strong>Potential relevance: </strong>Further study is warranted on the expression and effects of adipokines, particularly adiponectin, and correlation with postoperative outcome.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00404.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Implications of urine F2-isoprostane metabolite concentration in horses with colic and its potential use as a predictor for surgical intervention. 尿f2 -异前列腺素代谢物浓度对马绞痛的影响及其作为手术干预的潜在预测指标。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00384.x
E Noschka, S R Werre, M V Crisman, C D Thatcher, G L Milne, L A Dahlgren

Reasons for performing study: F2-isoprostanes have been used extensively to quantify lipid peroxidation in association with risk factors in various diseases. Horses with colic may have intestinal ischaemia and/or inflammation characterised by oxidative stress and increased production of isoprostanes.

Objectives: To gather preliminary data regarding the feasibility of using urine F2-isoprostanes and isoprostane metabolites as early screening tools for the presence of gastrointestinal disease requiring surgical intervention in horses and ultimately develop a stall-side test capable of identifying these horses as early as possible for timely referral.

Methods: Concentrations of urine isoprostane and isoprostane metabolite were determined by mass spectroscopy and normalised to urine creatinine (Cr) concentrations in urine samples from 42 healthy control horses and 43 horses with gastrointestinal pain or colic.

Results: Horses with colic were treated medically (n = 21) or surgically (n = 22). Mean ± s.d. concentrations of urine isoprostane and isoprostane metabolite were significantly higher in horses with colic (2.94 ± 1.69 and 0.31 ± 0.22 ng/mg Cr, respectively), compared to control horses (1.89 ± 1.39 and 0.22 ± 0.08 ng/mg Cr, respectively). Mean urine isoprostane metabolite concentrations were significantly higher in horses undergoing surgery (0.38 ± 0.28 ng/mg Cr) compared to controls and medical colics (0.26 ± 0.11 ng/mg Cr). Nonsurvivors had significantly higher mean urine isoprostane metabolite concentrations (0.47 ± 0.39 ng/mg Cr) than control or surviving colic horses (0.29 ± 0.24 ng/mg Cr).

Conclusions: Measurement of urine isoprostane metabolite concentration may be a useful prognostic indicator in equine colic.

Potential relevance: Urine isoprostane metabolites may aid in early recognition of surgical colic. Isoprostanes are a potential therapeutic target to prevent further systemic and gastrointestinal tissue injury in horses with colic.

研究原因:f2 -异前列腺素已被广泛用于定量脂质过氧化与各种疾病危险因素的关系。肠绞痛的马可能有肠道缺血和/或炎症,其特征是氧化应激和异前列腺素的产生增加。目的:收集关于使用尿液f2 -异前列腺素和异前列腺素代谢物作为需要手术干预的马胃肠道疾病早期筛查工具的可行性的初步数据,并最终开发一种能够尽早识别这些马并及时转诊的马厩侧测试。方法:采用质谱法测定42匹健康对照马和43匹胃肠道疼痛或绞痛马尿液中异前列腺素和异前列腺素代谢物的浓度,并将其与尿肌酐(Cr)浓度归一化。结果:经药物治疗(21例)或手术治疗(22例)。与对照组(1.89±1.39和0.22±0.08 ng/mg Cr)相比,绞痛马尿中异前列腺素和异前列腺素代谢物的平均±sd浓度(分别为2.94±1.69和0.31±0.22 ng/mg Cr)显著高于对照组(分别为2.94±1.69和0.31±0.22 ng/mg Cr)。手术马的尿异前列腺素代谢物平均浓度(0.38±0.28 ng/mg Cr)显著高于对照组和内科绞痛马(0.26±0.11 ng/mg Cr)。非幸存者的平均尿异前列腺素代谢物浓度(0.47±0.39 ng/mg Cr)显著高于对照组或幸存的绞痛马(0.29±0.24 ng/mg Cr)。结论:尿异前列腺素代谢物浓度的测定可能是马绞痛的一个有用的预后指标。潜在相关性:尿异前列腺素代谢物可能有助于外科绞痛的早期识别。异prostanes是一个潜在的治疗靶点,以防止进一步的系统和胃肠道组织损伤马绞痛。
{"title":"Implications of urine F2-isoprostane metabolite concentration in horses with colic and its potential use as a predictor for surgical intervention.","authors":"E Noschka,&nbsp;S R Werre,&nbsp;M V Crisman,&nbsp;C D Thatcher,&nbsp;G L Milne,&nbsp;L A Dahlgren","doi":"10.1111/j.2042-3306.2011.00384.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00384.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>F2-isoprostanes have been used extensively to quantify lipid peroxidation in association with risk factors in various diseases. Horses with colic may have intestinal ischaemia and/or inflammation characterised by oxidative stress and increased production of isoprostanes.</p><p><strong>Objectives: </strong>To gather preliminary data regarding the feasibility of using urine F2-isoprostanes and isoprostane metabolites as early screening tools for the presence of gastrointestinal disease requiring surgical intervention in horses and ultimately develop a stall-side test capable of identifying these horses as early as possible for timely referral.</p><p><strong>Methods: </strong>Concentrations of urine isoprostane and isoprostane metabolite were determined by mass spectroscopy and normalised to urine creatinine (Cr) concentrations in urine samples from 42 healthy control horses and 43 horses with gastrointestinal pain or colic.</p><p><strong>Results: </strong>Horses with colic were treated medically (n = 21) or surgically (n = 22). Mean ± s.d. concentrations of urine isoprostane and isoprostane metabolite were significantly higher in horses with colic (2.94 ± 1.69 and 0.31 ± 0.22 ng/mg Cr, respectively), compared to control horses (1.89 ± 1.39 and 0.22 ± 0.08 ng/mg Cr, respectively). Mean urine isoprostane metabolite concentrations were significantly higher in horses undergoing surgery (0.38 ± 0.28 ng/mg Cr) compared to controls and medical colics (0.26 ± 0.11 ng/mg Cr). Nonsurvivors had significantly higher mean urine isoprostane metabolite concentrations (0.47 ± 0.39 ng/mg Cr) than control or surviving colic horses (0.29 ± 0.24 ng/mg Cr).</p><p><strong>Conclusions: </strong>Measurement of urine isoprostane metabolite concentration may be a useful prognostic indicator in equine colic.</p><p><strong>Potential relevance: </strong>Urine isoprostane metabolites may aid in early recognition of surgical colic. Isoprostanes are a potential therapeutic target to prevent further systemic and gastrointestinal tissue injury in horses with colic.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00384.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The effect of nonsteroidal anti-inflammatory drugs on the equine intestine. 非甾体类抗炎药对马肠道的影响。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00398.x
J F Marshall, A T Blikslager

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in the management of pain and endotoxaemia associated with colic in the horse. While NSAIDs effectively treat the symptoms of colic, there is evidence to suggest that their administration is associated with adverse gastrointestinal effects including right dorsal colitis and inhibition of mucosal barrier healing. Several studies have examined the pathophysiology of NSAID associated effects on the large and small intestine in an effort to avoid these complications and identify effective alternative medications. Differences in the response of the large and small intestines to injury and NSAID treatment have been identified. Flunixin meglumine has been shown in the small intestine to inhibit barrier function recovery and increase permeability to lipopolysaccharide (LPS). A range of NSAIDs has been examined in the small intestine and experimental evidence suggests that those NSAIDs with cyclooxygenase independent anti-inflammatory effects or a COX-2 selective mode of action may offer significant advantages over traditional NSAIDs.

非甾体抗炎药(NSAIDs)通常用于治疗马绞痛引起的疼痛和内毒素血症。虽然非甾体抗炎药能有效治疗绞痛症状,但有证据表明,它们的使用与胃肠道不良反应有关,包括右背结肠炎和抑制粘膜屏障愈合。一些研究已经检查了非甾体抗炎药对大肠和小肠的病理生理学影响,以努力避免这些并发症并确定有效的替代药物。大肠和小肠对损伤和非甾体抗炎药治疗反应的差异已被确定。氟尼辛大聚胺在小肠中显示抑制屏障功能恢复和增加脂多糖(LPS)的通透性。一系列非甾体抗炎药已经在小肠中进行了研究,实验证据表明,那些具有环加氧酶独立抗炎作用或COX-2选择性作用模式的非甾体抗炎药可能比传统的非甾体抗炎药具有显著优势。
{"title":"The effect of nonsteroidal anti-inflammatory drugs on the equine intestine.","authors":"J F Marshall,&nbsp;A T Blikslager","doi":"10.1111/j.2042-3306.2011.00398.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00398.x","url":null,"abstract":"<p><p>Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in the management of pain and endotoxaemia associated with colic in the horse. While NSAIDs effectively treat the symptoms of colic, there is evidence to suggest that their administration is associated with adverse gastrointestinal effects including right dorsal colitis and inhibition of mucosal barrier healing. Several studies have examined the pathophysiology of NSAID associated effects on the large and small intestine in an effort to avoid these complications and identify effective alternative medications. Differences in the response of the large and small intestines to injury and NSAID treatment have been identified. Flunixin meglumine has been shown in the small intestine to inhibit barrier function recovery and increase permeability to lipopolysaccharide (LPS). A range of NSAIDs has been examined in the small intestine and experimental evidence suggests that those NSAIDs with cyclooxygenase independent anti-inflammatory effects or a COX-2 selective mode of action may offer significant advantages over traditional NSAIDs.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00398.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29890778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 66
Factors associated with survival of epiploic foramen entrapment colic: a multicentre, international study. 与大网膜孔夹持性绞痛存活相关的因素:一项多中心的国际研究。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00409.x
D C Archer, G L Pinchbeck, C J Proudman

Reasons for performing study: Epiploic foramen entrapment (EFE) has been associated with reduced post operative survival compared to other types of colic but specific factors associated with reduced long-term survival of these cases have not been evaluated in a large number of horses using survival analysis.

Objective: To describe post operative survival of EFE cases and to identify factors associated with long-term survival.

Methods: A prospective, multicentre, international study was conducted using clinical data and long-term follow-up information for 126 horses diagnosed with EFE during exploratory laparotomy at 15 clinics in the UK, Ireland and USA. Descriptive data were generated and survival analysis performed to identify factors associated with reduced post operative survival.

Results: For the EFE cohort that recovered following anaesthesia, survival to hospital discharge was 78.5%. Survival to 1 and 2 years post operatively was 50.6 and 34.3%, respectively. The median survival time of EFE cases undergoing surgery was 397 days. Increased packed cell volume (PCV) and increased length of small intestine (SI) resected were significantly associated with increased likelihood of mortality when multivariable analysis of pre- and intraoperative variables were analysed. When all pre-, intra- and post operative variables were analysed separately, only horses that developed post operative ileus (POI) were shown to be at increased likelihood of mortality.

Conclusions: Increased PCV, increased length of SI resected and POI are all associated with increased likelihood of mortality of EFE cases. This emphasises the importance of early diagnosis and treatment and the need for improved strategies in the management of POI in order to reduce post operative mortality in these cases.

Potential relevance: The present study provides evidence-based information to clinicians and owners of horses undergoing surgery for EFE about long-term survival. These results are applicable to university and large private clinics over a wide geographical area.

进行研究的原因:与其他类型的绞痛相比,epploic孔卡压(EFE)与术后生存率降低有关,但与这些病例的长期生存率降低相关的具体因素尚未在大量马中使用生存分析进行评估。目的:了解EFE患者的术后生存情况,并探讨影响其长期生存的因素。方法:在英国、爱尔兰和美国的15家诊所进行了一项前瞻性、多中心、国际研究,使用临床数据和长期随访信息对126匹在剖腹探查术中诊断为EFE的马进行了研究。生成描述性数据并进行生存分析,以确定与术后生存率降低相关的因素。结果:EFE组麻醉后恢复的患者,到出院的生存率为78.5%。术后1年和2年生存率分别为50.6%和34.3%。EFE患者接受手术的中位生存时间为397天。当对术前和术中变量进行多变量分析时,被切除的堆积细胞体积(PCV)增加和小肠长度(SI)增加与死亡率增加的可能性显著相关。当对所有术前、术中和术后变量进行单独分析时,只有发生术后肠梗阻(POI)的马显示出死亡率增加的可能性。结论:PCV增加、SI切除长度增加和POI都与EFE病例死亡率增加的可能性相关。这强调了早期诊断和治疗的重要性,以及改善POI管理策略的必要性,以降低这些病例的术后死亡率。潜在相关性:本研究为临床医生和接受EFE手术的马的主人提供了关于长期生存的循证信息。这些结果适用于广泛地理区域内的大学和大型私人诊所。
{"title":"Factors associated with survival of epiploic foramen entrapment colic: a multicentre, international study.","authors":"D C Archer,&nbsp;G L Pinchbeck,&nbsp;C J Proudman","doi":"10.1111/j.2042-3306.2011.00409.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00409.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Epiploic foramen entrapment (EFE) has been associated with reduced post operative survival compared to other types of colic but specific factors associated with reduced long-term survival of these cases have not been evaluated in a large number of horses using survival analysis.</p><p><strong>Objective: </strong>To describe post operative survival of EFE cases and to identify factors associated with long-term survival.</p><p><strong>Methods: </strong>A prospective, multicentre, international study was conducted using clinical data and long-term follow-up information for 126 horses diagnosed with EFE during exploratory laparotomy at 15 clinics in the UK, Ireland and USA. Descriptive data were generated and survival analysis performed to identify factors associated with reduced post operative survival.</p><p><strong>Results: </strong>For the EFE cohort that recovered following anaesthesia, survival to hospital discharge was 78.5%. Survival to 1 and 2 years post operatively was 50.6 and 34.3%, respectively. The median survival time of EFE cases undergoing surgery was 397 days. Increased packed cell volume (PCV) and increased length of small intestine (SI) resected were significantly associated with increased likelihood of mortality when multivariable analysis of pre- and intraoperative variables were analysed. When all pre-, intra- and post operative variables were analysed separately, only horses that developed post operative ileus (POI) were shown to be at increased likelihood of mortality.</p><p><strong>Conclusions: </strong>Increased PCV, increased length of SI resected and POI are all associated with increased likelihood of mortality of EFE cases. This emphasises the importance of early diagnosis and treatment and the need for improved strategies in the management of POI in order to reduce post operative mortality in these cases.</p><p><strong>Potential relevance: </strong>The present study provides evidence-based information to clinicians and owners of horses undergoing surgery for EFE about long-term survival. These results are applicable to university and large private clinics over a wide geographical area.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00409.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Ex vivo comparison of three hand sewn end-to-end anastomoses in normal equine jejunum. 正常马空肠三种手缝端对端吻合的离体比较。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00423.x
C Sherlock, W Lee, P O E Mueller, R Eggleston, K Epstein

Reasons for performing study: Although single layer techniques are preferred in man and small animals for small intestinal end-to-end anastomoses, double layer techniques are more popular in equine surgery. This study was undertaken to evaluate the ex vivo characteristics of 2 single layer anastomoses in comparison to the traditionally performed double layer anastomosis in equine jejunum.

Objectives: To compare ex vivo: 1) construction time; 2) bursting pressure; and 3) lumen size reduction of 3 suture patterns (double layer simple continuous/Cushing pattern [2C]; single layer Lembert pattern [1L]; and single layer Cushing pattern [1C]) for end-to-end equine jejunojejunostomies.

Methods: End-to-end jejunojejunostomies were constructed using 2C (n = 7), 1L (n = 7) and 1C (n = 7) in harvested equine jejunum and construction times were recorded. Anastomosed and control segments were distended with gas until failure. Intraluminal pressure at failure and mode of failure were recorded. Lumen size reduction was calculated as a percentage decrease from control jejunum. Results were compared using an ANOVA and P<0.05 was considered significant.

Results: The 1C anastomoses were faster to construct than the 1L anastomoses, which were faster to construct than the 2C anastomoses. There were no differences in bursting pressures between the different anastomoses and control jejunum. All anastomoses decreased lumen size from control values but there were no differences in lumen reduction between types of anastomoses.

Conclusions: Single layer anastomoses are faster to construct than double layer anastomoses, with the 1C being fastest. Single layer anastomoses are as strong and result in comparable lumen size reduction as traditional 2C anastomoses.

Potential relevance: As the 1C anastomosis results in less exposed potentially adhesiogenic suture material than the 1L while providing adequate strength and similar luminal size reduction, the 1C may be better for equine small intestine anastomosis and further in vivo studies are warranted.

开展研究的原因:虽然单层技术在人和小动物小肠端到端吻合术中更受青睐,但双层技术在马手术中更受欢迎。本研究旨在评价两层单层吻合与传统双层吻合在马空肠中的离体特性。目的:比较离体:1)构建时间;2)破裂压力;3) 3种缝合模式(双层简单连续/库欣模式)的管腔缩小[2C];单层Lembert模式[1L];和单层Cushing模式[1C])用于端到端马空肠吻合术。方法:采集马空肠,分别采用2C (n = 7)、1L (n = 7)和1C (n = 7)构建端到端空肠吻合术,记录构建时间。吻合段和对照段用气体膨胀直至失效。记录失效时的腔内压力和失效模式。肠腔大小减少以对照空肠减少的百分比计算。结果:1C吻合器的构建速度快于1L吻合器,1L吻合器的构建速度快于2C吻合器。不同吻合口与对照空肠的破裂压力无差异。所有的吻合口都比对照组缩小了管腔大小,但不同类型的吻合口在管腔缩小方面没有差异。结论:单层吻合器比双层吻合器构建速度快,且1C最快。单层吻合器与传统的2C吻合器一样坚固,并且可以减少相当的管腔大小。潜在相关性:1C吻合术比1L吻合术暴露的潜在粘连缝合材料更少,同时提供足够的强度和相似的管腔缩小,1C可能更适合马小肠吻合术,进一步的体内研究是有必要的。
{"title":"Ex vivo comparison of three hand sewn end-to-end anastomoses in normal equine jejunum.","authors":"C Sherlock,&nbsp;W Lee,&nbsp;P O E Mueller,&nbsp;R Eggleston,&nbsp;K Epstein","doi":"10.1111/j.2042-3306.2011.00423.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00423.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Although single layer techniques are preferred in man and small animals for small intestinal end-to-end anastomoses, double layer techniques are more popular in equine surgery. This study was undertaken to evaluate the ex vivo characteristics of 2 single layer anastomoses in comparison to the traditionally performed double layer anastomosis in equine jejunum.</p><p><strong>Objectives: </strong>To compare ex vivo: 1) construction time; 2) bursting pressure; and 3) lumen size reduction of 3 suture patterns (double layer simple continuous/Cushing pattern [2C]; single layer Lembert pattern [1L]; and single layer Cushing pattern [1C]) for end-to-end equine jejunojejunostomies.</p><p><strong>Methods: </strong>End-to-end jejunojejunostomies were constructed using 2C (n = 7), 1L (n = 7) and 1C (n = 7) in harvested equine jejunum and construction times were recorded. Anastomosed and control segments were distended with gas until failure. Intraluminal pressure at failure and mode of failure were recorded. Lumen size reduction was calculated as a percentage decrease from control jejunum. Results were compared using an ANOVA and P<0.05 was considered significant.</p><p><strong>Results: </strong>The 1C anastomoses were faster to construct than the 1L anastomoses, which were faster to construct than the 2C anastomoses. There were no differences in bursting pressures between the different anastomoses and control jejunum. All anastomoses decreased lumen size from control values but there were no differences in lumen reduction between types of anastomoses.</p><p><strong>Conclusions: </strong>Single layer anastomoses are faster to construct than double layer anastomoses, with the 1C being fastest. Single layer anastomoses are as strong and result in comparable lumen size reduction as traditional 2C anastomoses.</p><p><strong>Potential relevance: </strong>As the 1C anastomosis results in less exposed potentially adhesiogenic suture material than the 1L while providing adequate strength and similar luminal size reduction, the 1C may be better for equine small intestine anastomosis and further in vivo studies are warranted.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00423.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Investigation of the effect of pasture and stable management on large intestinal motility in the horse, measured using transcutaneous ultrasonography. 利用经皮超声检查研究牧场和马厩管理对马大肠运动的影响。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00399.x
S Williams, C A Tucker, M J Green, S L Freeman

Reason for performing study: Management regimes have been identified as risk factors for equine intestinal motility disorders. However, it is not known how management factors affect gastrointestinal motility.

Hypothesis: Large intestinal motility was similar in horses on a stabled and a pastured management regime.

Objective: To investigate the effect of 2 different management regimes on large intestinal motility assessed with ultrasonography.

Methods: A within-subjects crossover design was used to compare large intestinal motility between a stabled and a pastured regime in 16 working horses. Group A was managed under a standardised stabled regime throughout the study. Group B was maintained at pasture for the first monitoring phase and then transferred to the stabled regime for the second monitoring phase. Motility of the caecum, sternal flexure and aboral left ventral colon (contractions/min) was measured twice daily for 2 consecutive days using transcutaneous ultrasonography. Mean values for each management regime were pooled for analysis using multilevel statistical modelling.

Results: Significant variables identified by the model included: time of day, region of intestine, management regime, and combination of region of intestine and management regime. Motility assessed by ultrasound was significantly lower in stabled horses compared to pasture-kept horses. Intestinal motility for caecum was 1.7 ± 0.3 contractions/min (pastured = 2.0, stabled = 1.4), sternal flexure was 1.6 ± 0.2 contractions/min (mean (pastured = 1.7, stabled = 1.5), and left ventral colon was 0.8 ± 0.3 contractions/min (pastured = 1.0, stabled = 0.7).

Conclusions: The null hypothesis was disproven. Large intestinal motility assessed by ultrasound was significantly reduced in stabled horses compared to pastured horses. This effect was most marked in the aboral left ventral colon.

Potential relevance: This study has demonstrated a possible mechanism for the increased risk of large intestinal impactions in stabled horses.

开展研究的原因:管理制度已被确定为马肠道运动障碍的危险因素。然而,目前尚不清楚管理因素如何影响胃肠运动。假设:在马厩和牧场管理制度下,马的大肠运动是相似的。目的:探讨两种不同治疗方案对超声检查大肠运动的影响。方法:采用受试者内交叉设计比较16匹工作马在马厩和放牧状态下的大肠蠕动。在整个研究过程中,A组在标准化的稳定制度下进行管理。B组在第一监测阶段保持放牧,然后转入稳定状态进行第二监测阶段。采用经皮超声连续2天,每日2次测量盲肠运动、胸骨屈曲和离体左腹结肠(收缩/分钟)。每个管理制度的平均值使用多层统计模型进行汇总分析。结果:模型识别的显著变量包括:一天中的时间、肠道区域、管理制度以及肠道区域和管理制度的组合。通过超声波评估,马厩马的运动能力明显低于牧场马。盲肠肠蠕动1.7±0.3次收缩/min(放牧= 2.0,稳定= 1.4),胸骨屈曲1.6±0.2次收缩/min(平均(放牧= 1.7,稳定= 1.5),左腹结肠0.8±0.3次收缩/min(放牧= 1.0,稳定= 0.7)。结论:零假设被推翻。与放牧的马相比,通过超声波评估的马厩马的大肠运动明显减少。这种效果在离体左腹结肠中最为明显。潜在相关性:这项研究已经证明了马厩马大肠嵌塞风险增加的可能机制。
{"title":"Investigation of the effect of pasture and stable management on large intestinal motility in the horse, measured using transcutaneous ultrasonography.","authors":"S Williams,&nbsp;C A Tucker,&nbsp;M J Green,&nbsp;S L Freeman","doi":"10.1111/j.2042-3306.2011.00399.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00399.x","url":null,"abstract":"<p><strong>Reason for performing study: </strong>Management regimes have been identified as risk factors for equine intestinal motility disorders. However, it is not known how management factors affect gastrointestinal motility.</p><p><strong>Hypothesis: </strong>Large intestinal motility was similar in horses on a stabled and a pastured management regime.</p><p><strong>Objective: </strong>To investigate the effect of 2 different management regimes on large intestinal motility assessed with ultrasonography.</p><p><strong>Methods: </strong>A within-subjects crossover design was used to compare large intestinal motility between a stabled and a pastured regime in 16 working horses. Group A was managed under a standardised stabled regime throughout the study. Group B was maintained at pasture for the first monitoring phase and then transferred to the stabled regime for the second monitoring phase. Motility of the caecum, sternal flexure and aboral left ventral colon (contractions/min) was measured twice daily for 2 consecutive days using transcutaneous ultrasonography. Mean values for each management regime were pooled for analysis using multilevel statistical modelling.</p><p><strong>Results: </strong>Significant variables identified by the model included: time of day, region of intestine, management regime, and combination of region of intestine and management regime. Motility assessed by ultrasound was significantly lower in stabled horses compared to pasture-kept horses. Intestinal motility for caecum was 1.7 ± 0.3 contractions/min (pastured = 2.0, stabled = 1.4), sternal flexure was 1.6 ± 0.2 contractions/min (mean (pastured = 1.7, stabled = 1.5), and left ventral colon was 0.8 ± 0.3 contractions/min (pastured = 1.0, stabled = 0.7).</p><p><strong>Conclusions: </strong>The null hypothesis was disproven. Large intestinal motility assessed by ultrasound was significantly reduced in stabled horses compared to pastured horses. This effect was most marked in the aboral left ventral colon.</p><p><strong>Potential relevance: </strong>This study has demonstrated a possible mechanism for the increased risk of large intestinal impactions in stabled horses.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00399.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Special issue dedicated to Professor G. Berrie Edwards. 献给g·贝里·爱德华兹教授的特刊。
{"title":"Special issue dedicated to Professor G. Berrie Edwards.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30491442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An update on cyathostomins: anthelmintic resistance and diagnostic tools. cyathostomins的最新进展:驱虫虫耐药性和诊断工具。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00397.x
C H Stratford, B C McGorum, K J Pickles, J B Matthews

Cyathostomins represent a potential cause of equine morbidity and have become the main focus of endoparasite control in managed horses. All grazing horses are at risk of infection with cyathostomins; therefore, the application of appropriate management measures is essential. Anthelmintics currently comprise the main method of control for equine nematodes and the ready availability of these products in some countries has resulted in their use becoming dissociated from veterinary involvement. This is concerning given the levels of anthelmintic resistance that have been recorded in cyathostomin populations. It is important that veterinarians re-establish control over the implementation of parasite control programmes, a major objective of which should be the preservation of anthelmintic efficacy. This article details the principles of cyathostomin control in horses with particular reference to anthelmintic resistance, and the use and interpretation of diagnostic tests for detecting cyathostomins and identifying anthelmintic resistance.

Cyathostomins是马发病的潜在原因,已成为管理马体内寄生虫控制的主要焦点。所有吃草的马都有感染胞口毒素的危险;因此,采取适当的管理措施至关重要。驱虫药目前是控制马线虫的主要方法,在一些国家,这些产品的现成可用性使其使用与兽医参与分离开来。考虑到在胞口蛋白种群中记录的抗虫水平,这是令人担忧的。重要的是兽医对寄生虫控制规划的实施重新建立控制,其主要目标应是保持驱虫药的功效。本文详细介绍了在马体内控制cyathostomin的原则,特别是关于驱虫虫耐药性,以及用于检测cyathostomin和识别驱虫虫耐药性的诊断试验的使用和解释。
{"title":"An update on cyathostomins: anthelmintic resistance and diagnostic tools.","authors":"C H Stratford,&nbsp;B C McGorum,&nbsp;K J Pickles,&nbsp;J B Matthews","doi":"10.1111/j.2042-3306.2011.00397.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00397.x","url":null,"abstract":"<p><p>Cyathostomins represent a potential cause of equine morbidity and have become the main focus of endoparasite control in managed horses. All grazing horses are at risk of infection with cyathostomins; therefore, the application of appropriate management measures is essential. Anthelmintics currently comprise the main method of control for equine nematodes and the ready availability of these products in some countries has resulted in their use becoming dissociated from veterinary involvement. This is concerning given the levels of anthelmintic resistance that have been recorded in cyathostomin populations. It is important that veterinarians re-establish control over the implementation of parasite control programmes, a major objective of which should be the preservation of anthelmintic efficacy. This article details the principles of cyathostomin control in horses with particular reference to anthelmintic resistance, and the use and interpretation of diagnostic tests for detecting cyathostomins and identifying anthelmintic resistance.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00397.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29890777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 54
Multiple small intestinal pseudodiverticula associated with lymphoma in three horses. 三匹马多发性小肠假性憩室伴淋巴瘤。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00380.x
T S Mair, G R Pearson, T J Scase

Three mature horses presented with progressive weight loss, inappetence, ventral abdominal oedema and lethargy. Two of the animals had intermittent signs of low grade abdominal pain. At presentation, all 3 had hypoalbuminaemia; 2 had hyperfibrinogenaemia and the other had neutrophilia. An oral glucose tolerance test was performed in 2 cases, both of which demonstrated impaired glucose absorption. One pony treated with corticosteroids failed to improve and developed peritonitis and was subjected to euthanasia after 2 weeks. One pony had small intestinal biopsies obtained via a standing flank laparotomy, which revealed a mainly mononuclear cell infiltrate of the mucosa. It failed to respond to treatment with antibiotics and corticosteroids and, after 2 months, developed sternal oedema in addition to the ventral abdominal oedema and peritonitis and was subjected to euthanasia. The remaining pony deteriorated despite symptomatic therapy and was subjected to euthanasia after one week. At post mortem examination, all 3 animals had multifocal lesions of small intestinal wall thickening, mucosal ulceration, pseudodiverticula and enlarged mesenteric lymph nodes. One pony also had a multinodular mass at the root of the mesentery, a mediastinal mass and a lung mass. Histological examination confirmed the presence of lymphoma of the intestinal wall at post mortem examination in each case and immunohistochemistry (including retrospective evaluation of the intestinal biopsies obtained from the pony that underwent a flank laparotomy) indicated that the lymphomas were of T cell origin.

3匹成熟马表现出进行性体重减轻、食欲不振、腹侧水肿和嗜睡。其中两只动物有间歇性低度腹痛症状。3例患者均为低白蛋白血症;2例有高纤维蛋白原血症,1例有嗜中性粒细胞增多。2例患者均行口服葡萄糖耐量试验,均表现为葡萄糖吸收受损。一匹小马接受皮质类固醇治疗后,病情没有好转,出现腹膜炎,两周后被安乐死。一匹小马的小肠活检是通过站立剖腹手术获得的,结果显示粘膜主要是单核细胞浸润。抗生素和皮质类固醇治疗无效,2个月后,除腹侧水肿和腹膜炎外,还出现胸骨水肿,并被安乐死。尽管对症治疗,剩下的小马病情恶化,一周后被安乐死。尸检时,3只动物均出现小肠壁增厚、黏膜溃疡、假性憩室及肠系膜淋巴结肿大等多灶性病变。一匹小马在肠系膜根部也有一个多结节性肿块,一个纵隔肿块和一个肺肿块。组织学检查证实每例死后肠壁存在淋巴瘤,免疫组织化学(包括对接受腹部剖腹手术的小马肠道活检的回顾性评估)表明淋巴瘤是T细胞起源。
{"title":"Multiple small intestinal pseudodiverticula associated with lymphoma in three horses.","authors":"T S Mair,&nbsp;G R Pearson,&nbsp;T J Scase","doi":"10.1111/j.2042-3306.2011.00380.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00380.x","url":null,"abstract":"<p><p>Three mature horses presented with progressive weight loss, inappetence, ventral abdominal oedema and lethargy. Two of the animals had intermittent signs of low grade abdominal pain. At presentation, all 3 had hypoalbuminaemia; 2 had hyperfibrinogenaemia and the other had neutrophilia. An oral glucose tolerance test was performed in 2 cases, both of which demonstrated impaired glucose absorption. One pony treated with corticosteroids failed to improve and developed peritonitis and was subjected to euthanasia after 2 weeks. One pony had small intestinal biopsies obtained via a standing flank laparotomy, which revealed a mainly mononuclear cell infiltrate of the mucosa. It failed to respond to treatment with antibiotics and corticosteroids and, after 2 months, developed sternal oedema in addition to the ventral abdominal oedema and peritonitis and was subjected to euthanasia. The remaining pony deteriorated despite symptomatic therapy and was subjected to euthanasia after one week. At post mortem examination, all 3 animals had multifocal lesions of small intestinal wall thickening, mucosal ulceration, pseudodiverticula and enlarged mesenteric lymph nodes. One pony also had a multinodular mass at the root of the mesentery, a mediastinal mass and a lung mass. Histological examination confirmed the presence of lymphoma of the intestinal wall at post mortem examination in each case and immunohistochemistry (including retrospective evaluation of the intestinal biopsies obtained from the pony that underwent a flank laparotomy) indicated that the lymphomas were of T cell origin.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00380.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29890776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Effects of flunixin meglumine on the recovery of ischaemic equine colonic mucosa in vitro. 氟尼新大聚胺对马结肠缺血粘膜体外恢复的影响。
Pub Date : 2011-08-01 DOI: 10.1111/j.2042-3306.2011.00382.x
A J Morton, A Grosche, S A Matyjaszek, M M R Polyak, D E Freeman

Reasons for performing study: The effects of prostaglandins and nonsteroidal anti-inflammatory drugs (NSAIDs) on repair of equine intestinal mucosa are important since most horses with gastrointestinal diseases are routinely treated with NSAIDs, such as flunixin meglumine (FM), and these drugs can be toxic to equine gastrointestinal mucosa.

Hypothesis: Flunixin meglumine would not affect recovery of equine colonic mucosa in vitro, 18 h after a reversible ischaemic injury.

Methods: In 14 anaesthetised horses, a segment of pelvic flexure was subjected to 2 h of ischaemia and the horses were allowed to recover for 18 h. Seven horses received normal saline and 7 received FM, 1.1 mg/kg bwt i.v., at the end of ischaemia and 12 h later. Colonic mucosa was harvested during a second anaesthesia, 18 h after recovery from ischaemia and then horses were subjected to euthanasia. Transepithelial electrical resistance (TER) and transepithelial flux of tritiated mannitol were used to measure mucosal permeability during 4 h of incubation in Ussing chambers, with the following in vitro treatments: 1) no addition, 2) FM 14 µmol/l as powder, 3) FM 14 µmol/l in injectable form and 4) diluent for injectable FM. Histomorphological changes were assessed by light microscopy.

Results: There were no significant differences in any of the measurements between saline and FM treated horses. The mucosal height of the ischaemic FM tissues incubated in diluent was significantly decreased compared to the nonischaemic tissues.

Conclusions: Flunixin meglumine did not adversely affect barrier integrity in ischaemic equine colonic mucosa.

研究原因:前列腺素和非甾体抗炎药(NSAIDs)对马肠粘膜修复的作用是重要的,因为大多数患有胃肠道疾病的马常规使用非甾体抗炎药,如氟尼辛大明(FM),这些药物可能对马胃肠道粘膜有毒性。假设:氟尼新甲氨明不影响可逆缺血损伤后18小时体外马结肠黏膜的恢复。方法:14匹麻醉马,局部局部缺血2 h后恢复18 h,缺血结束后和12 h分别给予生理盐水7匹和FM 1.1 mg/kg bwt静脉注射。在缺血恢复后18小时的第二次麻醉中收集结肠黏膜,然后对马进行安乐死。用氚化甘露醇的经皮电阻(TER)和经皮通量测定在Ussing室培养4 h时的粘膜通透性,体外处理为:1)不添加,2)fm14µmol/l粉末,3)fm14µmol/l注射,4)fm14µmol/l注射稀释剂。光镜下观察组织形态学变化。结果:在生理盐水和FM治疗的马之间,任何测量都没有显着差异。与未缺血组织相比,用稀释液孵育的FM缺血组织的粘膜高度明显降低。结论:氟尼欣对缺血马结肠粘膜屏障完整性无不良影响。
{"title":"Effects of flunixin meglumine on the recovery of ischaemic equine colonic mucosa in vitro.","authors":"A J Morton,&nbsp;A Grosche,&nbsp;S A Matyjaszek,&nbsp;M M R Polyak,&nbsp;D E Freeman","doi":"10.1111/j.2042-3306.2011.00382.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00382.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>The effects of prostaglandins and nonsteroidal anti-inflammatory drugs (NSAIDs) on repair of equine intestinal mucosa are important since most horses with gastrointestinal diseases are routinely treated with NSAIDs, such as flunixin meglumine (FM), and these drugs can be toxic to equine gastrointestinal mucosa.</p><p><strong>Hypothesis: </strong>Flunixin meglumine would not affect recovery of equine colonic mucosa in vitro, 18 h after a reversible ischaemic injury.</p><p><strong>Methods: </strong>In 14 anaesthetised horses, a segment of pelvic flexure was subjected to 2 h of ischaemia and the horses were allowed to recover for 18 h. Seven horses received normal saline and 7 received FM, 1.1 mg/kg bwt i.v., at the end of ischaemia and 12 h later. Colonic mucosa was harvested during a second anaesthesia, 18 h after recovery from ischaemia and then horses were subjected to euthanasia. Transepithelial electrical resistance (TER) and transepithelial flux of tritiated mannitol were used to measure mucosal permeability during 4 h of incubation in Ussing chambers, with the following in vitro treatments: 1) no addition, 2) FM 14 µmol/l as powder, 3) FM 14 µmol/l in injectable form and 4) diluent for injectable FM. Histomorphological changes were assessed by light microscopy.</p><p><strong>Results: </strong>There were no significant differences in any of the measurements between saline and FM treated horses. The mucosal height of the ischaemic FM tissues incubated in diluent was significantly decreased compared to the nonischaemic tissues.</p><p><strong>Conclusions: </strong>Flunixin meglumine did not adversely affect barrier integrity in ischaemic equine colonic mucosa.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00382.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29890773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
期刊
Equine veterinary journal. Supplement
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1